2,3,5-Triphenyltetrazolium chloride, TTC
Catalog Number: ASC-1021
CAS Number: 298-96-4
MW: 334.80 g/mol
MF: C19H15ClN4
The dye 2,3,5-triphenyltetrazolium chloride (TTC) is primarily utilized for counting microbial colonies in solid culture media. When oxidized, this dye is colorless; when reduced by microbes by dehydrogenase enzyme, formazan is formed, which turns the dye red. Via enzymatic action, live microorganisms break down TTC, generating red formazan that is stored in cell granules. Dead cells stay colorless, whereas living cells turn red.
For Laboratory Use Only, Not For Human or Animal Usage.
2,3,5-Triphenyltetrazolium chloride (TTC; Tetrazolium chloride)
Cat. Number ASC-1021 CAS Number 298-96-4 MDL Number MFCD00011963 PubChem 310281422 Molecular Weight 334.80 g/mol Molecular Formula C19H15ClN4 Storage Temperature +4°C Form and Color White to faint yellow, Powder Solubility (5% in ethanol) Clear, Faint yellow to yellow solution Melting Point 238 – 243 °C Loss on Drying ≤ 3.0% (120°C, 3h) Assay ≥ 99.0% (dried basis) Synonym TT, TPTZ, TTZ, Tetrazolium Red