{"product_id":"2x-yt-agar","title":"2× YT Agar","description":"\u003c!--\nAusamics Product Page Standard v1.0 (Oct 2026)\nProduct: 2× YT Agar\nCatalogue No.: AS-1395\nManufacturer: Ausamics Pty Ltd, Melbourne, Australia\nCategory: Dehydrated Culture Medium\nPrimary use: Cultivation of recombinant Escherichia coli and propagation of M13 bacteriophage\n--\u003e\n\u003cdiv class=\"aus-pp\"\u003e\n\u003cstyle\u003e\n.aus-pp{container-type:inline-size;font-family:\"Segoe UI\",system-ui,-apple-system,Roboto,Arial,sans-serif;color:#1d2935;max-width:1200px;margin:0 auto;line-height:1.6;font-size:15px}\n.aus-pp *{box-sizing:border-box}\n.aus-pp h2,.aus-pp h3,.aus-pp h4,.aus-pp p{margin-top:0}\n.aus-pp em{font-style:italic}\n.aus-pp a{color:#1d6fb8}\n.aus-pp .faq{border-top:1px solid #e2e8f0;padding:12px 0}\n.aus-pp .faq:first-of-type{border-top:0;padding-top:0}\n.aus-pp .faq h4{font-size:15px;margin:0 0 4px}\n.aus-pp .faq p{margin:0}\n.aus-pp ul.links{margin:0;padding-left:18px}\n.aus-pp 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ul.list{margin:0;padding-left:20px}\n.aus-pp ul.list li,.aus-pp ol.refs li{margin-bottom:5px}\n.aus-pp ol.refs{padding-left:20px;font-size:14px;margin:0}\n.aus-pp .qc{background:linear-gradient(135deg,#f0f4f1,#c8d8cc);border-radius:14px;padding:18px 20px}\n.aus-pp .qc table{background:#fff}\n.aus-pp .box{border-radius:14px;padding:18px 20px;margin-bottom:18px;font-size:14px}\n.aus-pp .box p{margin:0}\n.aus-pp .s-white{background:#fff;border:1px solid #d8e1e8}\n.aus-pp .s-green{background:#f0f4f1;border:1px solid #d6e2d9}\n.aus-pp .s-gold{background:#fff9e6;border:2px solid #c9a84c}\n.aus-pp .specs td:first-child{background:#f6f8fb;font-weight:600;color:#15324b;width:46%}\n.aus-pp ol.steps{list-style:none;padding:0;margin:0;counter-reset:s}\n.aus-pp ol.steps li{counter-increment:s;position:relative;padding:0 0 12px 40px;font-size:14px}\n.aus-pp ol.steps li::before{content:counter(s);position:absolute;left:0;top:0;width:28px;height:28px;border-radius:50%;background:#2d5a3d;color:#fff;font-weight:700;font-size:13px;display:flex;align-items:center;justify-content:center}\n.aus-pp .info{background:#f0f4f1;border-left:4px solid #2d5a3d;border-radius:10px;padding:12px 16px;font-size:14px;margin:10px 0}\n.aus-pp .warn{background:#fff3e0;border-left:4px solid #e65100;border-radius:10px;padding:12px 16px;font-size:14px;margin:10px 0}\n.aus-pp .foot{background:#f8fafc;border:1px solid #e2e8f0;border-radius:12px;padding:14px 18px;font-size:13px;color:#52606d;margin-top:10px}\n@container (max-width:860px){.aus-pp .hero,.aus-pp .grid{grid-template-columns:1fr}.aus-pp .glance{min-width:0}.aus-pp .hero h2{font-size:24px}.aus-pp .tab-labels label{flex:1 1 45%;text-align:center;padding:10px 8px;font-size:13px}}\n.aus-pp #aas1395-t1:checked~.tab-labels label[for=aas1395-t1]{background:#fff;color:#2d5a3d;border-bottom:2px solid #fff;box-shadow:inset 0 3px 0 #2d5a3d}\n.aus-pp #aas1395-t2:checked~.tab-labels label[for=aas1395-t2]{background:#fff;color:#2d5a3d;border-bottom:2px solid #fff;box-shadow:inset 0 3px 0 #2d5a3d}\n.aus-pp #aas1395-t3:checked~.tab-labels label[for=aas1395-t3]{background:#fff;color:#2d5a3d;border-bottom:2px solid #fff;box-shadow:inset 0 3px 0 #2d5a3d}\n.aus-pp #aas1395-t4:checked~.tab-labels label[for=aas1395-t4]{background:#fff;color:#2d5a3d;border-bottom:2px solid #fff;box-shadow:inset 0 3px 0 #2d5a3d}\n.aus-pp #aas1395-t1:checked~.panels .p1{display:block}\n.aus-pp #aas1395-t2:checked~.panels .p2{display:block}\n.aus-pp #aas1395-t3:checked~.panels .p3{display:block}\n.aus-pp #aas1395-t4:checked~.panels .p4{display:block}\u003c\/style\u003e\n\u003cdiv class=\"hero\"\u003e\n\u003cdiv\u003e\n\u003cspan class=\"eyebrow\"\u003eDehydrated Culture Medium\u003c\/span\u003e\n\u003cp class=\"subtitle\"\u003eEnriched yeast extract–tryptone agar for high-density growth of recombinant \u003cem\u003eEscherichia coli\u003c\/em\u003e and M13 phage work\u003c\/p\u003e\n\u003cdiv class=\"badges\"\u003e\n\u003cspan class=\"badge b-solid\"\u003eCat. No. AS-1395\u003c\/span\u003e\u003cspan class=\"badge b-outline\"\u003e46 g\/L\u003c\/span\u003e\u003cspan class=\"badge b-outline\"\u003epH 7.0 ± 0.2\u003c\/span\u003e\u003cspan class=\"badge b-green\"\u003eNon-selective agar\u003c\/span\u003e\u003cspan class=\"badge b-gold\"\u003eMolecular biology\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"glance\"\u003e\n\u003ch4\u003eAt a glance\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003eTarget organisms\u003c\/span\u003e\u003cspan\u003e\u003cem\u003eE. coli\u003c\/em\u003e host strains\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eIncubation\u003c\/span\u003e\u003cspan\u003e35–37 °C, aerobic\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eTime\u003c\/span\u003e\u003cspan\u003e16–24 h\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eSterilisation\u003c\/span\u003e\u003cspan\u003e121 °C, 15 min\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv class=\"note\"\u003eManufactured in Melbourne. TDS and SDS available. Batch COA on request.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"tabs\"\u003e\n\u003cinput type=\"radio\" name=\"aas1395-tabs\" id=\"aas1395-t1\" checked\u003e\u003cinput type=\"radio\" name=\"aas1395-tabs\" id=\"aas1395-t2\"\u003e\u003cinput type=\"radio\" name=\"aas1395-tabs\" id=\"aas1395-t3\"\u003e\u003cinput type=\"radio\" name=\"aas1395-tabs\" id=\"aas1395-t4\"\u003e\u003cdiv class=\"tab-labels\"\u003e\n\u003clabel for=\"aas1395-t1\"\u003eProduct Description\u003c\/label\u003e\u003clabel for=\"aas1395-t2\"\u003eTechnical Data\u003c\/label\u003e\u003clabel for=\"aas1395-t3\"\u003eSafety Data\u003c\/label\u003e\u003clabel for=\"aas1395-t4\"\u003eQuality \u0026amp; COA\u003c\/label\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panels\"\u003e\n\u003cdiv class=\"panel p1\"\u003e\n\u003cdiv class=\"grid\"\u003e\n\u003cdiv\u003e\n\u003csection\u003e\u003ch3\u003eOverview\u003c\/h3\u003e\n\u003cp\u003e2× YT Agar (2× Yeast Extract Tryptone Agar) is an enriched, non-selective medium for growing recombinant \u003cem\u003eEscherichia coli\u003c\/em\u003e and for M13 bacteriophage work. The name comes from the original YT medium: 2× YT contains twice its tryptone and yeast extract (YT: 8 g\/L tryptone, 5 g\/L yeast extract).\u003c\/p\u003e\n\u003cp\u003eCompared with LB Agar (Miller), 2× YT Agar has more tryptone (16 vs 10 g\/L), twice the yeast extract (10 vs 5 g\/L) and half the sodium chloride (5 vs 10 g\/L). The richer nutrient base supports denser growth of \u003cem\u003eE. coli\u003c\/em\u003e host strains.\u003c\/p\u003e\n\u003ch4\u003eKey features\u003c\/h4\u003e\n\u003cul class=\"list\"\u003e\n\u003cli\u003eRicher than LB: 16 g\/L tryptone and 10 g\/L yeast extract\u003c\/li\u003e\n\u003cli\u003eA standard medium for M13 phage work and phage display hosts such as XL1-Blue and TG1\u003c\/li\u003e\n\u003cli\u003eAccepts antibiotics and other heat-labile supplements added after autoclaving\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003ePrinciple of the Medium\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eFunction\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTryptone\u003c\/td\u003e\n\u003ctd\u003ePeptides, amino acids and nitrogen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eYeast extract\u003c\/td\u003e\n\u003ctd\u003eB vitamins, nucleotides and growth factors\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium chloride\u003c\/td\u003e\n\u003ctd\u003eMaintains osmotic balance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003eSolidifying agent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cdiv class=\"info\"\u003eThe medium contains no selective agents. To select plasmid-bearing cells, add antibiotics such as ampicillin or kanamycin after autoclaving, once the medium has cooled to 45–50 °C.\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eTypical Composition\u003c\/h3\u003e\n\u003cdiv class=\"formula\"\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredient\u003c\/th\u003e\n\u003cth\u003eg\/L\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTryptone\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e16.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eYeast extract\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e10.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium chloride\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e5.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e15.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTotal\u003c\/th\u003e\n\u003cth\u003e46.00\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp class=\"small\" style=\"margin:8px 0 0\"\u003eFinal pH 7.0 ± 0.2 at 25 °C. Formula may be adjusted and\/or supplemented to meet performance specifications.\u003c\/p\u003e\n\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eField\u003c\/th\u003e\n\u003cth\u003eTypical use\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMolecular cloning\u003c\/td\u003e\n\u003ctd\u003eGrowth and selection of transformed \u003cem\u003eE. coli\u003c\/em\u003e on antibiotic plates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhage work\u003c\/td\u003e\n\u003ctd\u003ePropagation and titration of M13 bacteriophage; phage display with F′ host strains\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProtein expression\u003c\/td\u003e\n\u003ctd\u003eStreaking and maintaining expression strains such as BL21(DE3)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTeaching laboratories\u003c\/td\u003e\n\u003ctd\u003eTransformation and plasmid selection practicals\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eComparable Products\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eManufacturer\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eRelationship\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHiMedia Laboratories\u003c\/td\u003e\n\u003ctd\u003eG035 2XYT Growth Agar\u003c\/td\u003e\n\u003ctd\u003eSame published formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eFrequently Asked Questions\u003c\/h3\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eWhat is the difference between 2× YT Agar and LB Agar?\u003c\/h4\u003e\n\u003cp\u003e2× YT contains 16 g\/L tryptone and 10 g\/L yeast extract, compared with 10 g\/L and 5 g\/L in LB (Miller). It also has half the salt (5 vs 10 g\/L). The richer formula supports denser growth of \u003cem\u003eE. coli\u003c\/em\u003e.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eWhy is it called 2× YT?\u003c\/h4\u003e\n\u003cp\u003eIt contains twice the tryptone and yeast extract of the original YT medium (8 g\/L tryptone, 5 g\/L yeast extract).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eHow much powder do I need?\u003c\/h4\u003e\n\u003cp\u003e46 g per litre of purified water. A 500 g pack makes about 10.8 litres, or roughly 430–540 plates of 20–25 mL.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eWhen should I add antibiotics?\u003c\/h4\u003e\n\u003cp\u003eAfter autoclaving, once the agar has cooled to 45–50 °C. Adding them to hotter agar reduces their activity.\u003c\/p\u003e\n\u003c\/div\u003e\u003c\/section\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eTechnical Specifications\u003c\/h4\u003e\n\u003ctable class=\"specs\"\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalogue No.\u003c\/td\u003e\n\u003ctd\u003eAS-1395\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAlso known as\u003c\/td\u003e\n\u003ctd\u003e2xYT Agar; 2YT Agar; 2xTY Agar; 2× Yeast Extract Tryptone Agar\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMedium type\u003c\/td\u003e\n\u003ctd\u003eNon-selective agar\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUsage rate\u003c\/td\u003e\n\u003ctd\u003e46 g\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFinal pH (25 °C)\u003c\/td\u003e\n\u003ctd\u003e7.0 ± 0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePowder\u003c\/td\u003e\n\u003ctd\u003eCream to light yellow, free-flowing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrepared medium\u003c\/td\u003e\n\u003ctd\u003eLight yellow, clear to slightly opalescent gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-gold\"\u003e\n\u003ch4\u003ePreparation\u003c\/h4\u003e\n\u003col class=\"steps\"\u003e\n\u003cli\u003eSuspend 46 g in 1 L purified water.\u003c\/li\u003e\n\u003cli\u003eHeat to boiling with mixing until fully dissolved.\u003c\/li\u003e\n\u003cli\u003eAutoclave at 121 °C for 15 minutes.\u003c\/li\u003e\n\u003cli\u003eCool to 45–50 °C and add antibiotics if required.\u003c\/li\u003e\n\u003cli\u003eMix gently and pour 20–25 mL per 90 mm plate.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eStorage\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003ePowder:\u003c\/strong\u003e 10–30 °C, tightly closed, dry. Hygroscopic: reseal after use.\u003cbr\u003e\u003cstrong\u003ePrepared:\u003c\/strong\u003e 2–8 °C, protected from light.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-green\"\u003e\n\u003ch4\u003eRelated Products\u003c\/h4\u003e\n\u003cul class=\"links\"\u003e\n\u003cli\u003e\u003ca href=\"\/search?q=AS-1398\u0026amp;type=product\"\u003e2× YT Broth (AS-1398)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/search?q=AS-1267\u0026amp;type=product\"\u003eLB Agar, Miller (AS-1267)\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"\/search?q=AS-1268\u0026amp;type=product\"\u003eLB Agar, Lennox (AS-1268)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-green\"\u003e\n\u003ch4\u003eDocumentation\u003c\/h4\u003e\n\u003cul class=\"list\"\u003e\n\u003cli\u003eTechnical Data Sheet (TDS)\u003c\/li\u003e\n\u003cli\u003eSafety Data Sheet (SDS)\u003c\/li\u003e\n\u003cli\u003eBatch Certificate of Analysis (COA)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"foot\"\u003e\n\u003cstrong\u003eIntended use:\u003c\/strong\u003e For laboratory microbiological testing, quality control and research use only. Not intended for direct use in food, human or veterinary therapeutic applications, diagnosis, or consumption.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel p2\"\u003e\u003cdiv class=\"grid\"\u003e\n\u003cdiv\u003e\n\u003csection\u003e\u003ch3\u003eMode of Action\u003c\/h3\u003e\n\u003cp\u003eTryptone supplies peptides and amino acids, and yeast extract supplies B vitamins, nucleotides and other growth factors. The higher nutrient content than LB supports denser growth of \u003cem\u003eE. coli\u003c\/em\u003e. Sodium chloride maintains osmotic balance and agar solidifies the medium. The medium is non-selective; selection comes from antibiotics added after autoclaving.\u003c\/p\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003ePreparation\u003c\/h3\u003e\n\u003col class=\"steps\"\u003e\n\u003cli\u003eSuspend 46 g of powder in 1 litre of purified or distilled water.\u003c\/li\u003e\n\u003cli\u003eHeat to boiling with frequent mixing until completely dissolved.\u003c\/li\u003e\n\u003cli\u003eSterilise by autoclaving at 121 °C for 15 minutes.\u003c\/li\u003e\n\u003cli\u003eCool to 45–50 °C in a water bath.\u003c\/li\u003e\n\u003cli\u003eAdd filter-sterilised antibiotics or other supplements aseptically, if required, and mix gently.\u003c\/li\u003e\n\u003cli\u003ePour 20–25 mL into each 90 mm Petri dish and allow to set on a level surface.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv class=\"info\"\u003e\n\u003cstrong\u003eLiquid medium:\u003c\/strong\u003e 2× YT Broth uses the same formula without agar (31 g\/L).\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eIncubation and Interpretation\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eCondition\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAtmosphere\u003c\/td\u003e\n\u003ctd\u003eAerobic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTemperature\u003c\/td\u003e\n\u003ctd\u003e35–37 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTime\u003c\/td\u003e\n\u003ctd\u003e16–24 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePositive result\u003c\/td\u003e\n\u003ctd\u003eColonies on the agar surface\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp class=\"small\"\u003eGrowth does not identify an organism or confirm a clone. Confirm transformants by colony PCR, restriction digestion or sequencing as appropriate.\u003c\/p\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eLimitations\u003c\/h3\u003e\n\u003cul class=\"list\"\u003e\n\u003cli\u003eNon-selective: contaminating organisms will also grow unless antibiotics are added.\u003c\/li\u003e\n\u003cli\u003eAntibiotics are heat-labile; adding them above 50 °C reduces their activity.\u003c\/li\u003e\n\u003cli\u003eAmpicillin plates lose activity during storage, and long incubation (over 16–20 hours) can produce satellite colonies.\u003c\/li\u003e\n\u003cli\u003eGrowth rate varies between host strains.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eLiterature and References\u003c\/h3\u003e\n\u003col class=\"refs\"\u003e\n\u003cli\u003eSambrook J, Russell DW. \u003cem\u003eMolecular Cloning: A Laboratory Manual\u003c\/em\u003e, 3rd ed. Cold Spring Harbor Laboratory Press; 2001.\u003c\/li\u003e\n\u003cli\u003eGreen MR, Sambrook J. \u003cem\u003eMolecular Cloning: A Laboratory Manual\u003c\/em\u003e, 4th ed. Cold Spring Harbor Laboratory Press; 2012.\u003c\/li\u003e\n\u003cli\u003eMessing J. New M13 vectors for cloning. \u003cem\u003eMethods Enzymol\u003c\/em\u003e. 1983;101:20–78.\u003c\/li\u003e\n\u003cli\u003eBarbas CF III, Burton DR, Scott JK, Silverman GJ. \u003cem\u003ePhage Display: A Laboratory Manual\u003c\/em\u003e. Cold Spring Harbor Laboratory Press; 2001.\u003c\/li\u003e\n\u003cli\u003eAtlas RM. \u003cem\u003eHandbook of Microbiological Media\u003c\/em\u003e, 4th ed. CRC Press; 2010.\u003c\/li\u003e\n\u003c\/ol\u003e\u003c\/section\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003ePhysical Specifications\u003c\/h4\u003e\n\u003ctable class=\"specs\"\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance, powder\u003c\/td\u003e\n\u003ctd\u003eCream to light yellow, homogeneous, free-flowing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance, prepared\u003c\/td\u003e\n\u003ctd\u003eLight yellow, clear to slightly opalescent firm gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH (46 g\/L, 25 °C)\u003c\/td\u003e\n\u003ctd\u003e6.80–7.20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGel strength\u003c\/td\u003e\n\u003ctd\u003eFirm, comparable with a 1.5% agar gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-green\"\u003e\n\u003ch4\u003eRelated Products\u003c\/h4\u003e\n\u003cp\u003eLB Agar (Miller) (AS-1267) and LB Agar (Lennox) (AS-1268) are standard alternatives for routine cloning.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eStorage and Stability\u003c\/h4\u003e\n\u003cp\u003eStore powder at 10–30 °C, tightly closed and dry. Do not use if caked or discoloured. Prepared plates: 2–8 °C; shelf life to be established by the user.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003cdiv class=\"panel p3\"\u003e\u003cdiv class=\"grid\"\u003e\n\u003cdiv\u003e\n\u003csection\u003e\u003ch3\u003eHazard Identification\u003c\/h3\u003e\n\u003cdiv class=\"info\"\u003e\u003cstrong\u003eNot classified as hazardous according to the GHS (Rev. 7) as adopted in Australia under the model WHS Regulations. Not Dangerous Goods under the ADG Code.\u003c\/strong\u003e\u003c\/div\u003e\n\u003cp\u003eFine powder can form combustible dust concentrations in air. Dust may cause mild mechanical irritation of the eyes and respiratory tract. Contains casein-derived material: people with milk protein allergy should avoid exposure.\u003c\/p\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eComposition\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredient\u003c\/th\u003e\n\u003cth\u003eCAS No.\u003c\/th\u003e\n\u003cth\u003e% w\/w\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTryptone (enzymatic digest of casein)\u003c\/td\u003e\n\u003ctd\u003e91079-40-2\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e34.8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eYeast extract\u003c\/td\u003e\n\u003ctd\u003e8013-01-2\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e21.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium chloride\u003c\/td\u003e\n\u003ctd\u003e7647-14-5\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e10.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e9002-18-0\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e32.6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eFirst Aid\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eRoute\u003c\/th\u003e\n\u003cth\u003eAction\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInhalation\u003c\/td\u003e\n\u003ctd\u003eMove to fresh air. Seek medical advice if symptoms persist.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSkin\u003c\/td\u003e\n\u003ctd\u003eWash with soap and water.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEyes\u003c\/td\u003e\n\u003ctd\u003eRinse with water for several minutes. Seek medical advice if irritation persists.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIngestion\u003c\/td\u003e\n\u003ctd\u003eRinse mouth. Do not induce vomiting. Seek medical advice if unwell.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cdiv class=\"warn\"\u003e\u003cstrong\u003ePoisons Information Centre (Australia, 24 h): 13 11 26\u003c\/strong\u003e\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eHandling, Spills and Disposal\u003c\/h3\u003e\n\u003cul class=\"list\"\u003e\n\u003cli\u003eAvoid generating and breathing dust. Weigh in a ventilated area.\u003c\/li\u003e\n\u003cli\u003eSpills: sweep up or HEPA-vacuum without raising dust; wash the area with water.\u003c\/li\u003e\n\u003cli\u003eUnused product: dispose of as non-hazardous waste under local regulations.\u003c\/li\u003e\n\u003cli\u003eInoculated medium: treat as potentially biohazardous and decontaminate (for example by autoclaving) before disposal.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/section\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003ePersonal Protection\u003c\/h4\u003e\n\u003ctable class=\"specs\"\u003e\n\u003ctr\u003e\n\u003ctd\u003eEyes\u003c\/td\u003e\n\u003ctd\u003eSafety glasses with side shields\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHands\u003c\/td\u003e\n\u003ctd\u003eNitrile gloves\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBody\u003c\/td\u003e\n\u003ctd\u003eLaboratory coat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRespiratory\u003c\/td\u003e\n\u003ctd\u003eP2 respirator if dust is generated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-green\"\u003e\n\u003ch4\u003eFire and Stability\u003c\/h4\u003e\n\u003cp\u003eCombustible solid. Extinguish with water spray, foam, dry powder or CO\u003csub\u003e2\u003c\/sub\u003e. Stable under recommended storage. Avoid moisture, heat and strong oxidising agents.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-gold\"\u003e\n\u003ch4\u003eTransport\u003c\/h4\u003e\n\u003cp\u003eNot regulated for road, rail, sea or air transport. No UN number or Hazchem code.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eFull SDS\u003c\/h4\u003e\n\u003cp\u003eThe complete 16-section SDS is available on request from \u003ca href=\"mailto:support@ausamics.com?subject=SDS%20request%20AS-1395\"\u003esupport@ausamics.com\u003c\/a\u003e.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003cdiv class=\"panel p4\"\u003e\u003cdiv class=\"grid\"\u003e\n\u003cdiv\u003e\n\u003csection\u003e\u003ch3\u003eQuality Control Specification\u003c\/h3\u003e\n\u003cdiv class=\"qc\"\u003e\n\u003cp style=\"font-size:14px\"\u003eEach batch is tested for growth performance. Aerobic incubation at 35–37 °C for 18–24 hours.\u003c\/p\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable class=\"dark\"\u003e\n\u003ctr\u003e\n\u003cth\u003eQC organism\u003c\/th\u003e\n\u003cth\u003eStrain\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eExpected result\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cem\u003eEscherichia coli\u003c\/em\u003e\u003c\/td\u003e\n\u003ctd\u003eATCC 25922\u003c\/td\u003e\n\u003ctd\u003e50–100 (≤ 100)\u003c\/td\u003e\n\u003ctd\u003eGood to luxuriant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cem\u003eEscherichia coli\u003c\/em\u003e\u003c\/td\u003e\n\u003ctd\u003eATCC 23724\u003c\/td\u003e\n\u003ctd\u003e50–100 (≤ 100)\u003c\/td\u003e\n\u003ctd\u003eGood to luxuriant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eEscherichia coli\u003c\/em\u003e K-12\u003c\/td\u003e\n\u003ctd\u003eATCC 23716\u003c\/td\u003e\n\u003ctd\u003e50–100 (≤ 100)\u003c\/td\u003e\n\u003ctd\u003eGood to luxuriant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003ePhysical and Chemical Tests\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eTest\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance, powder\u003c\/td\u003e\n\u003ctd\u003eCream to light yellow, homogeneous, free-flowing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance, prepared medium\u003c\/td\u003e\n\u003ctd\u003eLight yellow, clear to slightly opalescent firm gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGel strength\u003c\/td\u003e\n\u003ctd\u003eFirm, comparable with a 1.5% agar gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH, prepared medium (25 °C)\u003c\/td\u003e\n\u003ctd\u003e6.80–7.20\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\u003c\/section\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv class=\"box s-gold\"\u003e\n\u003ch4\u003eCertificate of Analysis\u003c\/h4\u003e\n\u003cp style=\"margin-bottom:10px\"\u003eA batch-specific COA is available on request for every lot. It reports the lot number, manufacturing and expiry dates, and the results of the tests listed on this page.\u003c\/p\u003e\n\u003cp\u003eTo request a COA for a specific lot, email \u003ca href=\"mailto:support@ausamics.com?subject=COA%20request%20AS-1395\"\u003esupport@ausamics.com\u003c\/a\u003e with the lot number from your label.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eManufacturing\u003c\/h4\u003e\n\u003cp\u003eManufactured by Ausamics Pty Ltd in Melbourne, Australia.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"foot\"\u003eThe information on this page is provided in good faith as a guide to the properties and use of this product and does not constitute a warranty of fitness for a particular purpose. Users are responsible for confirming suitability for their application. Ausamics Pty Ltd · ABN 56 676 640 467 · 36\/25 Trafalgar Road, Epping VIC 3076, Australia · support@ausamics.com\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Ausamics Life Science","offers":[{"title":"100 g","offer_id":48044108546303,"sku":"AS-1395-100G","price":35.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":48044108611839,"sku":"AS-1395-500G","price":95.0,"currency_code":"AUD","in_stock":true},{"title":"2.5 kg","offer_id":48044108644607,"sku":"AS-1395-2.5KG","price":425.0,"currency_code":"AUD","in_stock":true},{"title":"10 kg","offer_id":48342189965567,"sku":"AS-1395-10KG","price":1450.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/2xYTAgar_AS-1395_Double-StrengthMediumforHigh-DensityBacterialGrowth.jpg?v=1774670091","url":"https:\/\/ausamics.com.au\/products\/2x-yt-agar","provider":"AuSaMicS","version":"1.0","type":"link"}