{"product_id":"acriflavine-hydrochloride","title":"Acriflavine Hydrochloride","description":"\u003c!--\nAusamics Product Page Standard v1.0 (Oct 2026)\nProduct: Acriflavine Hydrochloride\nCatalogue No.: ASA-2037\nSupplier: Ausamics Pty Ltd, Melbourne, Australia\nCategory: Acridine Dye \/ Research Biochemical\nPrimary use: Fluorescent nucleic acid stain and intercalating dye for laboratory research\n--\u003e\n\u003cdiv class=\"aus-pp\"\u003e\n\u003cstyle\u003e\n.aus-pp{container-type:inline-size;font-family:\"Segoe UI\",system-ui,-apple-system,Roboto,Arial,sans-serif;color:#1d2935;max-width:1200px;margin:0 auto;line-height:1.6;font-size:15px}\n.aus-pp *{box-sizing:border-box}\n.aus-pp h2,.aus-pp h3,.aus-pp h4,.aus-pp p{margin-top:0}\n.aus-pp em{font-style:italic}\n.aus-pp a{color:#1d6fb8}\n.aus-pp .faq{border-top:1px solid #e2e8f0;padding:12px 0}\n.aus-pp .faq:first-of-type{border-top:0;padding-top:0}\n.aus-pp .faq h4{font-size:15px;margin:0 0 4px}\n.aus-pp .faq p{margin:0}\n.aus-pp 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45%;text-align:center;padding:10px 8px;font-size:13px}}\n.aus-pp #asa2037-t1:checked~.tab-labels label[for=asa2037-t1]{background:#fff;color:#2d5a3d;border-bottom:2px solid #fff;box-shadow:inset 0 3px 0 #2d5a3d}\n.aus-pp #asa2037-t2:checked~.tab-labels label[for=asa2037-t2]{background:#fff;color:#2d5a3d;border-bottom:2px solid #fff;box-shadow:inset 0 3px 0 #2d5a3d}\n.aus-pp #asa2037-t3:checked~.tab-labels label[for=asa2037-t3]{background:#fff;color:#2d5a3d;border-bottom:2px solid #fff;box-shadow:inset 0 3px 0 #2d5a3d}\n.aus-pp #asa2037-t4:checked~.tab-labels label[for=asa2037-t4]{background:#fff;color:#2d5a3d;border-bottom:2px solid #fff;box-shadow:inset 0 3px 0 #2d5a3d}\n.aus-pp #asa2037-t1:checked~.panels .p1{display:block}\n.aus-pp #asa2037-t2:checked~.panels .p2{display:block}\n.aus-pp #asa2037-t3:checked~.panels .p3{display:block}\n.aus-pp #asa2037-t4:checked~.panels .p4{display:block}\u003c\/style\u003e\n\u003cdiv class=\"hero\"\u003e\n\u003cdiv\u003e\n\u003cspan class=\"eyebrow\"\u003eResearch Biochemical\u003c\/span\u003e\n\u003cp class=\"subtitle\"\u003eOrange acridine dye with strong nucleic-acid affinity, used as a fluorescent stain and intercalating probe in laboratory research\u003c\/p\u003e\n\u003cdiv class=\"badges\"\u003e\n\u003cspan class=\"badge b-solid\"\u003eCat. No. ASA-2037\u003c\/span\u003e\u003cspan class=\"badge b-outline\"\u003eCAS 8063-24-9\u003c\/span\u003e\u003cspan class=\"badge b-outline\"\u003e≥ 90% (HPLC)\u003c\/span\u003e\u003cspan class=\"badge b-outline\"\u003eAcridine dye\u003c\/span\u003e\u003cspan class=\"badge b-amber\"\u003eGHS: Danger\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"glance\"\u003e\n\u003ch4\u003eAt a glance\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003eAppearance\u003c\/span\u003e\u003cspan\u003eOrange to dark red powder\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eAbsorption max.\u003c\/span\u003e\u003cspan\u003e~452 nm\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eEmission\u003c\/span\u003e\u003cspan\u003e~505 nm\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eSolubility\u003c\/span\u003e\u003cspan\u003eWater and ethanol\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv class=\"note\"\u003eSupplied from Melbourne. TDS and SDS available. Batch COA on request.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"tabs\"\u003e\n\u003cinput type=\"radio\" name=\"asa2037-tabs\" id=\"asa2037-t1\" checked\u003e\u003cinput type=\"radio\" name=\"asa2037-tabs\" id=\"asa2037-t2\"\u003e\u003cinput type=\"radio\" name=\"asa2037-tabs\" id=\"asa2037-t3\"\u003e\u003cinput type=\"radio\" name=\"asa2037-tabs\" id=\"asa2037-t4\"\u003e\u003cdiv class=\"tab-labels\"\u003e\n\u003clabel for=\"asa2037-t1\"\u003eProduct Description\u003c\/label\u003e\u003clabel for=\"asa2037-t2\"\u003eTechnical Data\u003c\/label\u003e\u003clabel for=\"asa2037-t3\"\u003eSafety Data\u003c\/label\u003e\u003clabel for=\"asa2037-t4\"\u003eQuality \u0026amp; COA\u003c\/label\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panels\"\u003e\n\u003cdiv class=\"panel p1\"\u003e\n\u003cdiv class=\"grid\"\u003e\n\u003cdiv\u003e\n\u003csection\u003e\u003ch3\u003eOverview\u003c\/h3\u003e\n\u003cp\u003eAcriflavine hydrochloride is an acridine dye with a long history in microbiology and molecular biology. It is an intensely orange powder, soluble in water and ethanol, and it binds strongly to DNA and RNA.\u003c\/p\u003e\n\u003cp\u003eThe binding is what makes it useful. The acridine ring system is flat, and it slides between the stacked base pairs of a nucleic acid duplex, a mode of binding called intercalation. Once bound, the dye's fluorescence changes, so it reports on the nucleic acid it has attached to. That single property underlies its three main laboratory roles: as a fluorescent stain for visualising nucleic acids, as a probe in binding and intercalation studies, and as a fluorometric substrate in bacterial efflux-pump assays.\u003c\/p\u003e\n\u003cp\u003eThe acridines also occupy a particular place in the history of molecular genetics, having been the compounds used to establish that frameshift mutations arise from insertion and deletion of single bases. Acriflavine remains a reference intercalator in that work.\u003c\/p\u003e\n\u003cdiv class=\"warn\"\u003e\n\u003cstrong\u003eHazard note:\u003c\/strong\u003e this product carries the GHS signal word \u003cstrong\u003eDanger\u003c\/strong\u003e. It causes serious eye damage, causes skin irritation, is harmful if swallowed, and is toxic to aquatic life with long-lasting effects. As an intercalating dye it should also be handled and disposed of as potentially mutagenic. Read the Safety Data tab and the full SDS before ordering.\u003c\/div\u003e\n\u003ch4\u003eKey features\u003c\/h4\u003e\n\u003cul class=\"list\"\u003e\n\u003cli\u003eAssay ≥ 90% by HPLC\u003c\/li\u003e\n\u003cli\u003eStrong, well-characterised intercalative binding to double-stranded DNA\u003c\/li\u003e\n\u003cli\u003eSoluble in both water and ethanol, so it suits aqueous and part-organic systems\u003c\/li\u003e\n\u003cli\u003eFluorescence excitation near 452 nm fits standard blue-light and FITC-range optics\u003c\/li\u003e\n\u003cli\u003eEstablished substrate for multidrug efflux-pump activity measurements\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eIdentification\u003c\/h3\u003e\n\u003cdiv class=\"formula\"\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eProperty\u003c\/th\u003e\n\u003cth\u003eValue\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct name\u003c\/td\u003e\n\u003ctd\u003eAcriflavine hydrochloride\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCAS number\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e8063-24-9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eChemical class\u003c\/td\u003e\n\u003ctd\u003eAcridine dye\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eComposition\u003c\/td\u003e\n\u003ctd\u003eCommercial acriflavine is a mixture of acriflavinium chloride (3,6-diamino-10-methylacridinium chloride) and proflavine hydrochloride (3,6-diaminoacridine hydrochloride)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMolecular weight\u003c\/td\u003e\n\u003ctd\u003eNot applicable; the product is a mixture rather than a single compound\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAlso known as\u003c\/td\u003e\n\u003ctd\u003eAcriflavine HCl; acid acriflavine; trypaflavine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHS \/ AHECC code\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e2933.99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp class=\"small\" style=\"margin:8px 0 0\"\u003eBecause the product is a mixture, the ratio of the two components can differ between suppliers and affects both the spectra and the behaviour in quantitative assays. Where the ratio matters for your work, confirm it against the batch documentation. Note also that the name \"euflavine\" is generally applied to the pure acriflavinium salt and is not an exact synonym for this material.\u003c\/p\u003e\n\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eField\u003c\/th\u003e\n\u003cth\u003eTypical use\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFluorescence microscopy\u003c\/td\u003e\n\u003ctd\u003eStaining of nucleic acids in fixed cells and tissue sections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNucleic-acid biophysics\u003c\/td\u003e\n\u003ctd\u003eBinding, intercalation and helix-unwinding studies; reference intercalator in competition assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBacterial physiology\u003c\/td\u003e\n\u003ctd\u003eFluorometric substrate for measuring multidrug efflux-pump activity and accumulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMicrobial growth studies\u003c\/td\u003e\n\u003ctd\u003eGrowth-inhibition and resistance-mechanism work in culture systems\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMolecular genetics\u003c\/td\u003e\n\u003ctd\u003eReference frameshift mutagen in mutagenesis and DNA-repair research\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCell biology\u003c\/td\u003e\n\u003ctd\u003eCited in the research literature as an inhibitor of HIF-1 dimerisation in hypoxia-pathway studies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cdiv class=\"info\"\u003eWorking concentrations are strongly dependent on the assay, the sample type and the instrument, and published values span several orders of magnitude between applications. Titrate against your own system rather than adopting a figure from the literature unchanged.\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eFrequently Asked Questions\u003c\/h3\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eHow does it compare with ethidium bromide or acridine orange?\u003c\/h4\u003e\n\u003cp\u003eAll three are intercalating fluorescent dyes, and all three are handled as potentially mutagenic. Acriflavine absorbs in the blue, around 452 nm, and emits near 505 nm, so its optics sit in the FITC range rather than ethidium bromide's longer-wavelength window. Which one suits depends on your filter set and on what else you are staining.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eHow do I make up a stock solution?\u003c\/h4\u003e\n\u003cp\u003eDissolve in distilled or deionised water for aqueous work, or in ethanol for less polar systems. Keep stocks in amber or foil-wrapped tubes, since acridines photobleach, and store them at 4 °C. Make them up in small volumes so they are used while fresh.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eWhy is my fluorescence signal drifting during the measurement?\u003c\/h4\u003e\n\u003cp\u003eTwo effects usually account for it. The dye photobleaches under continued illumination, so minimise exposure and keep the illumination intensity consistent between samples. Its fluorescence is also quenched on binding, so a signal change may be the binding you are trying to measure rather than an artefact. Run an unbound-dye control to separate the two.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eHow should I dispose of spent solutions?\u003c\/h4\u003e\n\u003cp\u003eAs potentially mutagenic chemical waste, through your institution's hazardous waste route, the same way you would treat ethidium bromide. It is also classified as toxic to aquatic life with long-lasting effects, so it must not go down the drain.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eWhat PPE does it need?\u003c\/h4\u003e\n\u003cp\u003eChemical safety goggles rather than safety glasses, because the product causes serious eye damage, plus nitrile gloves and a fully fastened laboratory coat. Weigh the powder in a fume hood or ventilated enclosure. The dye also stains skin and fabric strongly on contact.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eIs it supplied for any therapeutic or veterinary purpose?\u003c\/h4\u003e\n\u003cp\u003eNo. This product is supplied for laboratory research use only. It is not supplied, labelled or specified for human, clinical, veterinary or aquaculture treatment use, and no such application is recommended.\u003c\/p\u003e\n\u003c\/div\u003e\u003c\/section\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv class=\"box s-amber\"\u003e\n\u003ch4\u003eHazard Summary\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003eSignal word: Danger\u003c\/strong\u003e\u003cbr\u003ePictograms: GHS05, GHS07, GHS09\u003cbr\u003eH302 — Harmful if swallowed.\u003cbr\u003eH315 — Causes skin irritation.\u003cbr\u003eH318 — Causes serious eye damage.\u003cbr\u003eH411 — Toxic to aquatic life with long-lasting effects.\u003cbr\u003e\u003cbr\u003eHandle as a potentially mutagenic intercalating dye. Goggles and gloves mandatory. Not to be released to drains.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eTechnical Specifications\u003c\/h4\u003e\n\u003ctable class=\"specs\"\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalogue No.\u003c\/td\u003e\n\u003ctd\u003eASA-2037\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGrade\u003c\/td\u003e\n\u003ctd\u003eResearch grade\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay\u003c\/td\u003e\n\u003ctd\u003e≥ 90.0% (HPLC)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance\u003c\/td\u003e\n\u003ctd\u003eOrange to dark red or brown crystalline powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLoss on drying\u003c\/td\u003e\n\u003ctd\u003e≤ 6.5%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSulfated ash\u003c\/td\u003e\n\u003ctd\u003e≤ 1.0%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolubility\u003c\/td\u003e\n\u003ctd\u003eSoluble in water and ethanol; insoluble in chloroform and ether\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHS \/ AHECC code\u003c\/td\u003e\n\u003ctd\u003e2933.99\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eStorage\u003c\/h4\u003e\n\u003cp\u003eStore at 2–8 °C, tightly closed, dry and protected from light. Keep locked up or in a restricted chemical store. Protect stock solutions from light and hold them at 4 °C.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-green\"\u003e\n\u003ch4\u003eDocumentation\u003c\/h4\u003e\n\u003cul class=\"list\"\u003e\n\u003cli\u003eTechnical Data Sheet (TDS)\u003c\/li\u003e\n\u003cli\u003eSafety Data Sheet (SDS)\u003c\/li\u003e\n\u003cli\u003eBatch Certificate of Analysis (COA)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"foot\"\u003e\n\u003cstrong\u003eIntended use:\u003c\/strong\u003e For laboratory research use only. Not for human or animal consumption. Not intended for therapeutic, clinical, diagnostic, veterinary, aquaculture or food applications. No therapeutic or medicinal properties are claimed for this product.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel p2\"\u003e\u003cdiv class=\"grid\"\u003e\n\u003cdiv\u003e\n\u003csection\u003e\u003ch3\u003eMode of Action\u003c\/h3\u003e\n\u003cp\u003eAcriflavine is a planar tricyclic acridine cation. It binds double-stranded DNA by intercalation, inserting between adjacent stacked base pairs roughly perpendicular to the helical axis, which locally lengthens and unwinds the duplex. Binding is driven by stacking interactions with the bases and by the electrostatic attraction between the cationic dye and the polyanionic backbone, so it is sensitive to ionic strength.\u003c\/p\u003e\n\u003cp\u003eIntercalation accounts for both of the dye's uses. The change in the fluorophore's environment on binding alters its fluorescence, which is the basis of its use as a stain and as a binding probe. Distorting the helix also interferes with replication and transcription, which is the basis of its growth-inhibitory effect on microorganisms and of its classical activity as a frameshift mutagen.\u003c\/p\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003ePhysical and Spectral Properties\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eProperty\u003c\/th\u003e\n\u003cth\u003eValue\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance\u003c\/td\u003e\n\u003ctd\u003eOrange to dark red or brown crystalline powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAbsorption maximum\u003c\/td\u003e\n\u003ctd\u003eapproximately 452 nm in aqueous solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEmission maximum\u003c\/td\u003e\n\u003ctd\u003eapproximately 505 nm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolubility, water\u003c\/td\u003e\n\u003ctd\u003eSoluble\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolubility, ethanol\u003c\/td\u003e\n\u003ctd\u003eSoluble\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolubility, chloroform and ether\u003c\/td\u003e\n\u003ctd\u003eInsoluble\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMelting behaviour\u003c\/td\u003e\n\u003ctd\u003eDecomposes on heating rather than melting cleanly\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhotostability\u003c\/td\u003e\n\u003ctd\u003ePhotobleaches under sustained illumination\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHygroscopicity\u003c\/td\u003e\n\u003ctd\u003eHygroscopic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp class=\"small\"\u003eSpectral maxima shift with solvent, pH, ionic strength and on binding to nucleic acid. The figures above are approximate values for the free dye in water and should be confirmed on your own instrument for quantitative work.\u003c\/p\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eHandling Guidance\u003c\/h3\u003e\n\u003col class=\"steps\"\u003e\n\u003cli\u003eWeigh the powder in a fume hood or ventilated weighing enclosure, wearing chemical safety goggles, nitrile gloves and a fastened laboratory coat.\u003c\/li\u003e\n\u003cli\u003ePrepare stock solutions in distilled or deionised water, or in ethanol for less polar systems. Protect them from light from the moment they are made.\u003c\/li\u003e\n\u003cli\u003eStore stock and working solutions at 4 °C in amber or foil-wrapped containers. Prepare small volumes so they are used while fresh.\u003c\/li\u003e\n\u003cli\u003eTitrate the working concentration empirically for your staining or assay system. Optimal loading varies with sample type, detection method and instrument sensitivity.\u003c\/li\u003e\n\u003cli\u003eMinimise illumination time during measurement, and keep exposure consistent across samples, to limit photobleaching.\u003c\/li\u003e\n\u003cli\u003eCollect all dye-containing waste, including stained material and rinse water, for disposal as potentially mutagenic hazardous waste. Nothing goes to drain.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv class=\"info\"\u003e\n\u003cstrong\u003eOn quantitative work:\u003c\/strong\u003e because this product is a mixture of two acridines rather than a single compound, molar calculations based on a single molecular weight will be inexact. For work that depends on accurate concentration, determine it spectrophotometrically against a standard curve prepared from the same batch.\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eLimitations\u003c\/h3\u003e\n\u003cul class=\"list\"\u003e\n\u003cli\u003eThe product is a mixture, so the component ratio can vary between batches and suppliers and may affect quantitative results.\u003c\/li\u003e\n\u003cli\u003eThe dye photobleaches, which limits long time-course fluorescence measurements and repeated imaging of the same field.\u003c\/li\u003e\n\u003cli\u003eFluorescence is sensitive to pH, ionic strength and binding state, so controls must be run under the sample conditions rather than in buffer alone.\u003c\/li\u003e\n\u003cli\u003eIntercalating dyes bind nucleic acid non-specifically and do not distinguish sequence or, on their own, DNA from RNA.\u003c\/li\u003e\n\u003cli\u003eAll dye-containing waste requires a hazardous waste route, which adds cost and handling compared with non-intercalating alternatives.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eLiterature and References\u003c\/h3\u003e\n\u003col class=\"refs\"\u003e\n\u003cli\u003ePeacocke AR, Skerrett JNH. The interaction of aminoacridines with nucleic acids. \u003cem\u003eTrans Faraday Soc\u003c\/em\u003e. 1956;52:261–279.\u003c\/li\u003e\n\u003cli\u003eLerman LS. Structural considerations in the interaction of DNA and acridines. \u003cem\u003eJ Mol Biol\u003c\/em\u003e. 1961;3(1):18–30.\u003c\/li\u003e\n\u003cli\u003eCrick FHC, Barnett L, Brenner S, Watts-Tobin RJ. General nature of the genetic code for proteins. \u003cem\u003eNature\u003c\/em\u003e. 1961;192:1227–1232.\u003c\/li\u003e\n\u003cli\u003eWainwright M. Acridine — a neglected antibacterial chromophore. \u003cem\u003eJ Antimicrob Chemother\u003c\/em\u003e. 2001;47(1):1–13.\u003c\/li\u003e\n\u003cli\u003eLee K, Zhang H, Qian DZ, Rey S, Liu JO, Semenza GL. Acriflavine inhibits HIF-1 dimerization, tumor growth, and vascularization. \u003cem\u003eProc Natl Acad Sci USA\u003c\/em\u003e. 2009;106(42):17910–17915.\u003c\/li\u003e\n\u003cli\u003eBlair JMA, Piddock LJV. How to measure export via bacterial multidrug resistance efflux pumps. \u003cem\u003emBio\u003c\/em\u003e. 2016;7(4):e00840-16.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp class=\"small\"\u003eReferences are provided for technical context. Citation of a published study is not a claim about this product's performance or suitability for that application.\u003c\/p\u003e\u003c\/section\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv class=\"box s-amber\"\u003e\n\u003ch4\u003eBefore You Order\u003c\/h4\u003e\n\u003cp\u003eConfirm that your laboratory has a fume hood or ventilated enclosure for weighing, chemical safety goggles available to users, a hazardous waste route for mutagenic dye waste including rinse water, and a risk assessment covering intercalating dyes. If any of these is missing, resolve it before the product arrives rather than after.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eTypical Specification\u003c\/h4\u003e\n\u003ctable class=\"specs\"\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay (HPLC)\u003c\/td\u003e\n\u003ctd\u003e≥ 90.0%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance\u003c\/td\u003e\n\u003ctd\u003eOrange to dark red or brown crystalline powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLoss on drying (105 °C)\u003c\/td\u003e\n\u003ctd\u003e≤ 6.5%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSulfated ash\u003c\/td\u003e\n\u003ctd\u003e≤ 1.0%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolubility, water and ethanol\u003c\/td\u003e\n\u003ctd\u003eSoluble\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIdentification\u003c\/td\u003e\n\u003ctd\u003eConforms to reference spectrum\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eStorage and Stability\u003c\/h4\u003e\n\u003cp\u003eStable as a dry powder under the recommended conditions. Store at 2–8 °C, tightly closed, dry and protected from light. Avoid strong oxidising agents. Aqueous and ethanolic stock solutions should be protected from light, held at 4 °C and prepared in small volumes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003cdiv class=\"panel p3\"\u003e\u003cdiv class=\"grid\"\u003e\n\u003cdiv\u003e\n\u003csection\u003e\u003ch3\u003eHazard Identification\u003c\/h3\u003e\n\u003cdiv class=\"warn\"\u003e\n\u003cstrong\u003eClassified as hazardous according to the GHS (Rev. 7) as adopted in Australia under the model WHS Regulations.\u003c\/strong\u003e\u003cbr\u003eAcute Toxicity (Oral), Category 4. Skin Irritation, Category 2. Serious Eye Damage, Category 1. Hazardous to the Aquatic Environment (Chronic), Category 2.\u003cbr\u003eSignal word: \u003cstrong\u003eDanger\u003c\/strong\u003e. Pictograms: GHS05 (corrosion), GHS07 (exclamation mark), GHS09 (environment).\u003c\/div\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eCode\u003c\/th\u003e\n\u003cth\u003eHazard statement\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eH302\u003c\/td\u003e\n\u003ctd\u003eHarmful if swallowed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eH315\u003c\/td\u003e\n\u003ctd\u003eCauses skin irritation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eH318\u003c\/td\u003e\n\u003ctd\u003eCauses serious eye damage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eH411\u003c\/td\u003e\n\u003ctd\u003eToxic to aquatic life with long-lasting effects\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePrecautionary statements:\u003c\/strong\u003e P264 Wash thoroughly after handling. P270 Do not eat, drink or smoke when using this product. P273 Avoid release to the environment. P280 Wear protective gloves, protective clothing and eye protection. P301+P312+P330 IF SWALLOWED: call a poison centre or doctor if you feel unwell; rinse mouth. P305+P351+P338+P310 IF IN EYES: rinse cautiously with water for several minutes, remove contact lenses if present and easy to do, continue rinsing, and immediately call a poison centre or doctor. P332+P313 If skin irritation occurs, get medical advice. P391 Collect spillage. P501 Dispose of contents and container in accordance with local regulations.\u003c\/p\u003e\n\u003cdiv class=\"warn\"\u003e\n\u003cstrong\u003eMutagenicity.\u003c\/strong\u003e Acriflavine is a well-characterised DNA-intercalating agent and frameshift mutagen in experimental systems. This property is not reflected in the formal GHS classification above. Handle the product, its solutions, stained material and rinse water as potentially mutagenic waste, consistent with institutional practice for intercalating dyes such as ethidium bromide.\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eComposition\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCAS No.\u003c\/th\u003e\n\u003cth\u003eContent\u003c\/th\u003e\n\u003cth\u003eClassification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcriflavine hydrochloride\u003c\/td\u003e\n\u003ctd\u003e8063-24-9\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e≥ 90%\u003c\/td\u003e\n\u003ctd\u003eAcute Tox. 4 (H302); Skin Irrit. 2 (H315); Eye Dam. 1 (H318); Aquatic Chronic 2 (H411)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eFirst Aid\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eRoute\u003c\/th\u003e\n\u003cth\u003eAction\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInhalation\u003c\/td\u003e\n\u003ctd\u003eMove to fresh air and keep at rest. If breathing is difficult, seek medical attention.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSkin\u003c\/td\u003e\n\u003ctd\u003eRemove contaminated clothing. Wash the skin thoroughly with plenty of water. Seek medical advice if irritation persists. Residual staining may remain after the dye has been washed off and is not in itself a sign of injury.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEyes\u003c\/td\u003e\n\u003ctd\u003eRinse cautiously with water for several minutes. Remove contact lenses if present and easy to do, then continue rinsing. Immediately call a Poisons Information Centre or a doctor: this product causes serious eye damage.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIngestion\u003c\/td\u003e\n\u003ctd\u003eRinse mouth. Do not induce vomiting unless directed by medical personnel. Call a Poisons Information Centre or doctor and show the label or SDS.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cdiv class=\"warn\"\u003e\u003cstrong\u003ePoisons Information Centre (Australia, 24 h): 13 11 26\u003c\/strong\u003e\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eHandling, Spills and Disposal\u003c\/h3\u003e\n\u003cul class=\"list\"\u003e\n\u003cli\u003eHandle in a fume hood or ventilated weighing enclosure. Avoid generating dust.\u003c\/li\u003e\n\u003cli\u003eDo not eat, drink or smoke in the handling area. Wash hands thoroughly after use.\u003c\/li\u003e\n\u003cli\u003eStore locked up or in a restricted chemical store, segregated from oxidising agents.\u003c\/li\u003e\n\u003cli\u003eProtect surfaces before opening the container; the dye stains strongly and spreads easily.\u003c\/li\u003e\n\u003cli\u003eSpills: avoid dust. Collect dry material mechanically or by HEPA vacuum into a labelled closed container, then wash the area and collect the washings as chemical waste. Do not wash a dry spill first, as this spreads it.\u003c\/li\u003e\n\u003cli\u003eEnvironmental precautions: prevent entry to drains, waterways and sewers. The product is toxic to aquatic life with long-lasting effects.\u003c\/li\u003e\n\u003cli\u003eDisposal: dispose of the product, its solutions, contaminated packaging, stained material and rinse water as hazardous chemical waste through a licensed contractor, in accordance with local regulations.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/section\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv class=\"box s-amber\"\u003e\n\u003ch4\u003ePersonal Protection\u003c\/h4\u003e\n\u003ctable class=\"specs\"\u003e\n\u003ctr\u003e\n\u003ctd\u003eEngineering control\u003c\/td\u003e\n\u003ctd\u003eFume hood or local exhaust ventilation for powder handling\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEyes\u003c\/td\u003e\n\u003ctd\u003eChemical safety goggles, not spectacles — required for the serious eye damage hazard\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHands\u003c\/td\u003e\n\u003ctd\u003eNitrile gloves; replace promptly if contaminated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBody\u003c\/td\u003e\n\u003ctd\u003eLaboratory coat, fully fastened\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRespiratory\u003c\/td\u003e\n\u003ctd\u003eP2 respirator where dust cannot be controlled by ventilation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-green\"\u003e\n\u003ch4\u003eFire and Stability\u003c\/h4\u003e\n\u003cp\u003eCombustible solid. Extinguish with water spray, foam, dry powder or CO\u003csub\u003e2\u003c\/sub\u003e. Combustion produces carbon monoxide, carbon dioxide and nitrogen oxides; wear self-contained breathing apparatus when fighting a fire involving this product. Contain fire water, which will be contaminated and must not reach drains or waterways. Stable under recommended storage. Avoid light, moisture and strong oxidising agents.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-gold\"\u003e\n\u003ch4\u003eTransport\u003c\/h4\u003e\n\u003cp\u003eNot classified as Dangerous Goods under the ADG Code, IATA or IMDG at typical laboratory pack sizes. Confirm classification for bulk shipments, as the aquatic hazard can bring larger quantities into scope as an environmentally hazardous substance.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eFull SDS\u003c\/h4\u003e\n\u003cp\u003eThe complete 16-section SDS is available on request from \u003ca href=\"mailto:support@ausamics.com?subject=SDS%20request%20ASA-2037\"\u003esupport@ausamics.com\u003c\/a\u003e. Obtain and read it before the product is ordered.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003cdiv class=\"panel p4\"\u003e\u003cdiv class=\"grid\"\u003e\n\u003cdiv\u003e\n\u003csection\u003e\u003ch3\u003eQuality Control Specification\u003c\/h3\u003e\n\u003cdiv class=\"qc\"\u003e\n\u003cp style=\"font-size:14px\"\u003eEach batch is supplied against the following specification. Results for the specific lot are reported on the batch Certificate of Analysis.\u003c\/p\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable class=\"dark\"\u003e\n\u003ctr\u003e\n\u003cth\u003eTest\u003c\/th\u003e\n\u003cth\u003eMethod\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAssay\u003c\/td\u003e\n\u003ctd\u003eHPLC\u003c\/td\u003e\n\u003ctd\u003e≥ 90.0%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance\u003c\/td\u003e\n\u003ctd\u003eVisual\u003c\/td\u003e\n\u003ctd\u003eOrange to dark red or brown crystalline powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLoss on drying\u003c\/td\u003e\n\u003ctd\u003eGravimetric, 105 °C\u003c\/td\u003e\n\u003ctd\u003e≤ 6.5%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSulfated ash\u003c\/td\u003e\n\u003ctd\u003eGravimetric\u003c\/td\u003e\n\u003ctd\u003e≤ 1.0%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolubility, water\u003c\/td\u003e\n\u003ctd\u003eVisual clarity\u003c\/td\u003e\n\u003ctd\u003eSoluble\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolubility, ethanol\u003c\/td\u003e\n\u003ctd\u003eVisual clarity\u003c\/td\u003e\n\u003ctd\u003eSoluble\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIdentification\u003c\/td\u003e\n\u003ctd\u003eUV-visible spectrum\u003c\/td\u003e\n\u003ctd\u003eConforms to reference\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eIdentity Confirmation\u003c\/h3\u003e\n\u003cp\u003eIdentity is confirmed by the UV-visible absorption spectrum of an aqueous solution against a reference, with the principal absorption maximum at approximately 452 nm.\u003c\/p\u003e\n\u003cdiv class=\"info\"\u003eBecause the product is a mixture of two acridines, the assay figure is a measure of total acridine content rather than of a single chemical entity. Where the ratio of the two components affects your work, request the batch documentation before ordering.\u003c\/div\u003e\u003c\/section\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv class=\"box s-gold\"\u003e\n\u003ch4\u003eCertificate of Analysis\u003c\/h4\u003e\n\u003cp style=\"margin-bottom:10px\"\u003eA batch-specific COA is available on request for every lot. It reports the lot number, manufacturing and expiry dates, and the results of the tests listed on this page.\u003c\/p\u003e\n\u003cp\u003eTo request a COA for a specific lot, email \u003ca href=\"mailto:support@ausamics.com?subject=COA%20request%20ASA-2037\"\u003esupport@ausamics.com\u003c\/a\u003e with the lot number from your label.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eSupply\u003c\/h4\u003e\n\u003cp\u003eSupplied by Ausamics Pty Ltd, Melbourne, Australia.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"foot\"\u003eThe information on this page is provided in good faith as a guide to the properties and use of this product and does not constitute a warranty of fitness for a particular purpose. Users are responsible for confirming suitability for their application and for complying with all applicable workplace health and safety requirements. Ausamics Pty Ltd · ABN 56 676 640 467 · 36\/25 Trafalgar Road, Epping VIC 3076, Australia · support@ausamics.com\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Ausamics Life Science","offers":[{"title":"2.5 g","offer_id":48046074298623,"sku":"ASA-2037-2.5G","price":27.0,"currency_code":"AUD","in_stock":true},{"title":"5 g","offer_id":48046074331391,"sku":"ASA-2037-5G","price":49.0,"currency_code":"AUD","in_stock":true},{"title":"10 g","offer_id":48046074364159,"sku":"ASA-2037-10G","price":67.0,"currency_code":"AUD","in_stock":true},{"title":"25 g","offer_id":48046074396927,"sku":"ASA-2037-25G","price":99.0,"currency_code":"AUD","in_stock":true},{"title":"50 g","offer_id":48046074429695,"sku":"ASA-2037-50G","price":145.0,"currency_code":"AUD","in_stock":true},{"title":"100 g","offer_id":48046074462463,"sku":"ASA-2037-100G","price":195.0,"currency_code":"AUD","in_stock":true},{"title":"250 g","offer_id":48046074495231,"sku":"ASA-2037-250G","price":375.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/AcriflavineHCL.jpg?v=1771892918","url":"https:\/\/ausamics.com.au\/products\/acriflavine-hydrochloride","provider":"AuSaMicS","version":"1.0","type":"link"}