{"product_id":"actinomycete-isolation-agar","title":"Actinomycete Isolation Agar (AC Agar)","description":"\u003c!--\nAusamics Product Page Standard v1.0 (Oct 2026)\nProduct: Actinomycete Isolation Agar (AC Agar)\nCatalogue No.: AS-1102\nManufacturer: Ausamics Pty Ltd, Melbourne, Australia\nCategory: Dehydrated Culture Medium\nPrimary use: Selective isolation of actinomycetes from soil and water\n--\u003e\n\u003cdiv class=\"aus-pp\"\u003e\n\u003cstyle\u003e\n.aus-pp{container-type:inline-size;font-family:\"Segoe UI\",system-ui,-apple-system,Roboto,Arial,sans-serif;color:#1d2935;max-width:1200px;margin:0 auto;line-height:1.6;font-size:15px}\n.aus-pp *{box-sizing:border-box}\n.aus-pp h2,.aus-pp h3,.aus-pp h4,.aus-pp p{margin-top:0}\n.aus-pp em{font-style:italic}\n.aus-pp a{color:#1d6fb8}\n.aus-pp .faq{border-top:1px solid #e2e8f0;padding:12px 0}\n.aus-pp .faq:first-of-type{border-top:0;padding-top:0}\n.aus-pp .faq h4{font-size:15px;margin:0 0 4px}\n.aus-pp .faq p{margin:0}\n.aus-pp 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table{margin:0}\n.aus-pp .small{font-size:13px;color:#52606d}\n.aus-pp ul.list{margin:0;padding-left:20px}\n.aus-pp ul.list li,.aus-pp ol.refs li{margin-bottom:5px}\n.aus-pp ol.refs{padding-left:20px;font-size:14px;margin:0}\n.aus-pp .qc{background:linear-gradient(135deg,#f0f4f1,#c8d8cc);border-radius:14px;padding:18px 20px}\n.aus-pp .qc table{background:#fff}\n.aus-pp .box{border-radius:14px;padding:18px 20px;margin-bottom:18px;font-size:14px}\n.aus-pp .box p{margin:0}\n.aus-pp .s-white{background:#fff;border:1px solid #d8e1e8}\n.aus-pp .s-green{background:#f0f4f1;border:1px solid #d6e2d9}\n.aus-pp .s-gold{background:#fff9e6;border:2px solid #c9a84c}\n.aus-pp .specs td:first-child{background:#f6f8fb;font-weight:600;color:#15324b;width:46%}\n.aus-pp ol.steps{list-style:none;padding:0;margin:0;counter-reset:s}\n.aus-pp ol.steps li{counter-increment:s;position:relative;padding:0 0 12px 40px;font-size:14px}\n.aus-pp ol.steps li::before{content:counter(s);position:absolute;left:0;top:0;width:28px;height:28px;border-radius:50%;background:#2d5a3d;color:#fff;font-weight:700;font-size:13px;display:flex;align-items:center;justify-content:center}\n.aus-pp .info{background:#f0f4f1;border-left:4px solid #2d5a3d;border-radius:10px;padding:12px 16px;font-size:14px;margin:10px 0}\n.aus-pp .warn{background:#fff3e0;border-left:4px solid #e65100;border-radius:10px;padding:12px 16px;font-size:14px;margin:10px 0}\n.aus-pp .foot{background:#f8fafc;border:1px solid #e2e8f0;border-radius:12px;padding:14px 18px;font-size:13px;color:#52606d;margin-top:10px}\n@container (max-width:860px){.aus-pp .hero,.aus-pp .grid{grid-template-columns:1fr}.aus-pp .glance{min-width:0}.aus-pp .hero h2{font-size:24px}.aus-pp .tab-labels label{flex:1 1 45%;text-align:center;padding:10px 8px;font-size:13px}}\n.aus-pp #aas1102-t1:checked~.tab-labels label[for=aas1102-t1]{background:#fff;color:#2d5a3d;border-bottom:2px solid #fff;box-shadow:inset 0 3px 0 #2d5a3d}\n.aus-pp #aas1102-t2:checked~.tab-labels label[for=aas1102-t2]{background:#fff;color:#2d5a3d;border-bottom:2px solid #fff;box-shadow:inset 0 3px 0 #2d5a3d}\n.aus-pp #aas1102-t3:checked~.tab-labels label[for=aas1102-t3]{background:#fff;color:#2d5a3d;border-bottom:2px solid #fff;box-shadow:inset 0 3px 0 #2d5a3d}\n.aus-pp #aas1102-t4:checked~.tab-labels label[for=aas1102-t4]{background:#fff;color:#2d5a3d;border-bottom:2px solid #fff;box-shadow:inset 0 3px 0 #2d5a3d}\n.aus-pp #aas1102-t1:checked~.panels .p1{display:block}\n.aus-pp #aas1102-t2:checked~.panels .p2{display:block}\n.aus-pp #aas1102-t3:checked~.panels .p3{display:block}\n.aus-pp #aas1102-t4:checked~.panels .p4{display:block}\u003c\/style\u003e\n\u003cdiv class=\"hero\"\u003e\n\u003cdiv\u003e\n\u003cspan class=\"eyebrow\"\u003eDehydrated Culture Medium\u003c\/span\u003e\n\u003cp class=\"subtitle\"\u003eSodium propionate selective agar for isolating \u003cem\u003eStreptomyces\u003c\/em\u003e and other actinomycetes from soil, water and sediment\u003c\/p\u003e\n\u003cdiv class=\"badges\"\u003e\n\u003cspan class=\"badge b-solid\"\u003eCat. No. AS-1102\u003c\/span\u003e\u003cspan class=\"badge b-outline\"\u003e21.7 g\/L\u003c\/span\u003e\u003cspan class=\"badge b-outline\"\u003epH 8.1 ± 0.2\u003c\/span\u003e\u003cspan class=\"badge b-green\"\u003eSelective agar\u003c\/span\u003e\u003cspan class=\"badge b-gold\"\u003eSoil \u0026amp; water microbiology\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"glance\"\u003e\n\u003ch4\u003eAt a glance\u003c\/h4\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cspan\u003eTarget organisms\u003c\/span\u003e\u003cspan\u003eActinomycetes\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eIncubation\u003c\/span\u003e\u003cspan\u003e25–30 °C, aerobic\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eTime\u003c\/span\u003e\u003cspan\u003e7–21 days\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eSterilisation\u003c\/span\u003e\u003cspan\u003e121 °C, 15 min\u003c\/span\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv class=\"note\"\u003eManufactured in Melbourne. TDS and SDS available. Batch COA on request.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"tabs\"\u003e\n\u003cinput type=\"radio\" name=\"aas1102-tabs\" id=\"aas1102-t1\" checked\u003e\u003cinput type=\"radio\" name=\"aas1102-tabs\" id=\"aas1102-t2\"\u003e\u003cinput type=\"radio\" name=\"aas1102-tabs\" id=\"aas1102-t3\"\u003e\u003cinput type=\"radio\" name=\"aas1102-tabs\" id=\"aas1102-t4\"\u003e\u003cdiv class=\"tab-labels\"\u003e\n\u003clabel for=\"aas1102-t1\"\u003eProduct Description\u003c\/label\u003e\u003clabel for=\"aas1102-t2\"\u003eTechnical Data\u003c\/label\u003e\u003clabel for=\"aas1102-t3\"\u003eSafety Data\u003c\/label\u003e\u003clabel for=\"aas1102-t4\"\u003eQuality \u0026amp; COA\u003c\/label\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panels\"\u003e\n\u003cdiv class=\"panel p1\"\u003e\n\u003cdiv class=\"grid\"\u003e\n\u003cdiv\u003e\n\u003csection\u003e\u003ch3\u003eOverview\u003c\/h3\u003e\n\u003cp\u003eActinomycete Isolation Agar (AC Agar) is a selective solid medium for isolating and cultivating actinomycetes, including \u003cem\u003eStreptomyces\u003c\/em\u003e and related genera, from soil, water, sediment and other mixed environmental samples.\u003c\/p\u003e\n\u003cp\u003eIsolating these organisms by direct plating is difficult because they grow slowly. On a rich non-selective agar, a soil sample's fast-growing bacteria and fungi cover the plate within a day or two, long before an actinomycete has formed a visible colony. This medium solves that by holding the competitors back. Sodium propionate inhibits many Gram-negative bacteria and fast-growing fungi, the nitrogen supply is deliberately restricted to sources actinomycetes use well, and the pH is set at 8.1, above the comfortable range of many soil organisms.\u003c\/p\u003e\n\u003cp\u003eGiven a long enough incubation, actinomycetes then develop their characteristic small, dry, powdery colonies with aerial mycelium, and the plate stays readable.\u003c\/p\u003e\n\u003ch4\u003eKey features\u003c\/h4\u003e\n\u003cul class=\"list\"\u003e\n\u003cli\u003eSodium propionate as the selective agent, with no antibiotic required\u003c\/li\u003e\n\u003cli\u003eAlkaline pH 8.1 adds a second layer of selectivity\u003c\/li\u003e\n\u003cli\u003eRestricted nitrogen base favours actinomycetes over competing soil organisms\u003c\/li\u003e\n\u003cli\u003eSupports aerial mycelium and sporulation, so colony morphology is diagnostic\u003c\/li\u003e\n\u003cli\u003eGlycerol, added separately, is a preferred carbon source for \u003cem\u003eStreptomyces\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli\u003eSuits slants for stock maintenance as well as plates for isolation\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003ePrinciple of the Medium\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eFunction\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium caseinate\u003c\/td\u003e\n\u003ctd\u003ePrimary nitrogen source; poorly used by many competing organisms\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Asparagine\u003c\/td\u003e\n\u003ctd\u003eSupplementary nitrogen and amino acids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium propionate\u003c\/td\u003e\n\u003ctd\u003eSelective agent; inhibits many Gram-negative bacteria and fungi\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDipotassium phosphate\u003c\/td\u003e\n\u003ctd\u003eBuffers the medium at alkaline pH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnesium sulfate\u003c\/td\u003e\n\u003ctd\u003eEnzyme cofactor source\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFerrous sulfate\u003c\/td\u003e\n\u003ctd\u003eTrace iron for respiratory and biosynthetic enzymes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003eSolidifying agent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGlycerol (added separately)\u003c\/td\u003e\n\u003ctd\u003eCarbon and energy source; supports aerial mycelium formation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cdiv class=\"info\"\u003eSelectivity comes from the formulation itself, not from an antibiotic. For samples with heavy mould loads, add filter-sterilised cycloheximide or nystatin after autoclaving, once the agar has cooled to 45–50 °C.\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eTypical Composition\u003c\/h3\u003e\n\u003cdiv class=\"formula\"\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredient\u003c\/th\u003e\n\u003cth\u003eg\/L\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e15.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium propionate\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e4.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium caseinate\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e2.000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDipotassium phosphate\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e0.500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Asparagine\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e0.100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnesium sulfate heptahydrate\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e0.100\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFerrous sulfate heptahydrate\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e0.001\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTotal\u003c\/th\u003e\n\u003cth\u003e21.701\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp class=\"small\" style=\"margin:8px 0 0\"\u003eFinal pH 8.1 ± 0.2 at 25 °C. Glycerol (5 mL\/L) is not included in the dehydrated powder and is added by the user. Formula may be adjusted and\/or supplemented to meet performance specifications.\u003c\/p\u003e\n\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eApplications\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eField\u003c\/th\u003e\n\u003cth\u003eTypical use\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSoil microbiology\u003c\/td\u003e\n\u003ctd\u003eIsolation and enumeration of actinomycetes from soil and compost\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater and sediment testing\u003c\/td\u003e\n\u003ctd\u003eRecovery of actinomycetes from water and sediment samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNatural-product screening\u003c\/td\u003e\n\u003ctd\u003eScreening soil isolates for strains producing bioactive compounds\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCulture maintenance\u003c\/td\u003e\n\u003ctd\u003eSubculturing and stock maintenance of actinomycete isolates on slants\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTeaching laboratories\u003c\/td\u003e\n\u003ctd\u003eSoil and environmental microbiology practicals\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eComparable Products\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eManufacturer\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eRelationship\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBD Difco\u003c\/td\u003e\n\u003ctd\u003e212168 Actinomycete Isolation Agar\u003c\/td\u003e\n\u003ctd\u003eSame published formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHiMedia Laboratories\u003c\/td\u003e\n\u003ctd\u003eM490 Actinomycete Isolation Agar\u003c\/td\u003e\n\u003ctd\u003eSame published formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp class=\"small\"\u003eComparison is based on published formulation and intended use. Users should confirm performance under their own conditions.\u003c\/p\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eFrequently Asked Questions\u003c\/h3\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eHow much powder do I need, and where does the glycerol go?\u003c\/h4\u003e\n\u003cp\u003e21.7 g of powder per litre of purified water, plus 5 mL of glycerol added before autoclaving. The glycerol is not part of the dehydrated blend. Prepared without it, the medium has no usable carbon source and growth will be poor.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eHow long do the plates need to incubate?\u003c\/h4\u003e\n\u003cp\u003e7 to 21 days at 25–30 °C. Check periodically rather than reading once at the end, because colony morphology changes as aerial mycelium develops and because a plate left too long may be overgrown by a faster isolate.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eWhat do actinomycete colonies look like on this medium?\u003c\/h4\u003e\n\u003cp\u003eSmall, dry and powdery, often tough and firmly attached to the agar rather than easily picked off. Many develop a white to grey aerial mycelium as they sporulate, and some produce diffusible pigment that colours the surrounding agar.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eWill the plates dry out over three weeks?\u003c\/h4\u003e\n\u003cp\u003eThey can. Pour a full 20–25 mL per 90 mm plate, store the plates in sealed bags or a humidified container, and incubate them inverted. Plates poured thin will crack or shrink before the incubation is finished.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eShould I use the agar or the broth?\u003c\/h4\u003e\n\u003cp\u003eUse the agar when you want single colonies and can plate the sample directly. Use AC Broth (AS-1103) first when the target organisms are too scarce to recover by direct plating, then plate the enriched culture onto this agar.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq\"\u003e\n\u003ch4\u003eDo I need to add an antifungal?\u003c\/h4\u003e\n\u003cp\u003eNot for every sample. The base formulation suppresses many fungi. For soil or compost with a heavy mould load, add filter-sterilised cycloheximide at 50 mg\/L after autoclaving, and nystatin at 50 mg\/L as well if moulds still break through.\u003c\/p\u003e\n\u003c\/div\u003e\u003c\/section\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eTechnical Specifications\u003c\/h4\u003e\n\u003ctable class=\"specs\"\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalogue No.\u003c\/td\u003e\n\u003ctd\u003eAS-1102\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAlso known as\u003c\/td\u003e\n\u003ctd\u003eAC Agar; Actinomycete Isolation Agar; \u003cem\u003eStreptomyces\u003c\/em\u003e isolation medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMedium type\u003c\/td\u003e\n\u003ctd\u003eSelective agar\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUsage rate\u003c\/td\u003e\n\u003ctd\u003e21.7 g\/L plus 5 mL glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFinal pH (25 °C)\u003c\/td\u003e\n\u003ctd\u003e8.1 ± 0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLoss on drying\u003c\/td\u003e\n\u003ctd\u003e≤ 6.0% w\/w\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePowder\u003c\/td\u003e\n\u003ctd\u003eCream to light beige, free-flowing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrepared medium\u003c\/td\u003e\n\u003ctd\u003ePale straw, clear to slightly opalescent firm gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHS \/ AHECC code\u003c\/td\u003e\n\u003ctd\u003e3821.00.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-gold\"\u003e\n\u003ch4\u003ePreparation\u003c\/h4\u003e\n\u003col class=\"steps\"\u003e\n\u003cli\u003eSuspend 21.7 g in 1 L purified water.\u003c\/li\u003e\n\u003cli\u003eAdd 5 mL glycerol.\u003c\/li\u003e\n\u003cli\u003eHeat to boiling with mixing until fully dissolved.\u003c\/li\u003e\n\u003cli\u003eAutoclave at 121 °C for 15 minutes.\u003c\/li\u003e\n\u003cli\u003eCool to 45–50 °C and add antifungal agents if required.\u003c\/li\u003e\n\u003cli\u003eMix gently and pour 20–25 mL per 90 mm plate.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eStorage\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003ePowder:\u003c\/strong\u003e 10–30 °C, tightly closed, dry and protected from light. Hygroscopic: reseal after use.\u003cbr\u003e\u003cstrong\u003ePrepared:\u003c\/strong\u003e 2–8 °C, protected from light and from drying out.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-green\"\u003e\n\u003ch4\u003eRelated Products\u003c\/h4\u003e\n\u003cul class=\"links\"\u003e\n\u003cli\u003e\u003ca href=\"\/products\/ac-broth-actinomycete-isolation-broth\"\u003eAC Broth, Actinomycete Isolation Broth (AS-1103)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-green\"\u003e\n\u003ch4\u003eDocumentation\u003c\/h4\u003e\n\u003cul class=\"list\"\u003e\n\u003cli\u003eTechnical Data Sheet (TDS)\u003c\/li\u003e\n\u003cli\u003eSafety Data Sheet (SDS)\u003c\/li\u003e\n\u003cli\u003eBatch Certificate of Analysis (COA)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"foot\"\u003e\n\u003cstrong\u003eIntended use:\u003c\/strong\u003e For laboratory microbiological testing, quality control and research use only. Not intended for direct use in food, human or veterinary therapeutic applications, diagnosis, or consumption.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"panel p2\"\u003e\u003cdiv class=\"grid\"\u003e\n\u003cdiv\u003e\n\u003csection\u003e\u003ch3\u003eMode of Action\u003c\/h3\u003e\n\u003cp\u003eSodium caseinate and L-asparagine supply nitrogen in forms that actinomycetes use readily and many competing organisms use poorly. Sodium propionate is the selective agent, inhibiting a broad range of Gram-negative bacteria and fast-growing fungi. Dipotassium phosphate buffers the medium at pH 8.1, which further restricts competitors, and magnesium and ferrous sulfate supply essential cations. Glycerol, added by the user, is the carbon and energy source that drives aerial mycelium formation and sporulation at the agar surface.\u003c\/p\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003ePreparation\u003c\/h3\u003e\n\u003col class=\"steps\"\u003e\n\u003cli\u003eSuspend 21.7 g of powder in 1 litre of purified or distilled water.\u003c\/li\u003e\n\u003cli\u003eAdd 5 mL of glycerol.\u003c\/li\u003e\n\u003cli\u003eHeat to boiling with frequent mixing until the agar is completely dissolved.\u003c\/li\u003e\n\u003cli\u003eSterilise by autoclaving at 121 °C for 15 minutes.\u003c\/li\u003e\n\u003cli\u003eCool to 45–50 °C in a water bath.\u003c\/li\u003e\n\u003cli\u003eAdd filter-sterilised cycloheximide (50 mg\/L) and, if required, nystatin (50 mg\/L), and mix gently.\u003c\/li\u003e\n\u003cli\u003ePour 20–25 mL into each 90 mm Petri dish and allow to set on a level surface.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv class=\"info\"\u003e\n\u003cstrong\u003eLiquid medium:\u003c\/strong\u003e AC Broth (AS-1103) uses the same base without agar, at 6.7 g\/L plus glycerol.\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eDirections for Use\u003c\/h3\u003e\n\u003cp\u003eInoculate the agar surface with 0.1 mL of a diluted soil or water suspension and spread with a sterile spreader. Allow the surface to dry before inverting. Incubate aerobically and examine periodically for small, dry, powdery colonies, which typically become visible after 5 to 7 days and continue to develop aerial mycelium over the following two weeks.\u003c\/p\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eCondition\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAtmosphere\u003c\/td\u003e\n\u003ctd\u003eAerobic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTemperature\u003c\/td\u003e\n\u003ctd\u003e25–30 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTime\u003c\/td\u003e\n\u003ctd\u003e7–21 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePositive result\u003c\/td\u003e\n\u003ctd\u003eSmall, dry, powdery colonies, often with aerial mycelium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp class=\"small\"\u003eColony morphology on this medium is suggestive but not confirmatory. Identify isolates by microscopy, biochemical characterisation and 16S rRNA gene sequencing as appropriate.\u003c\/p\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eLimitations\u003c\/h3\u003e\n\u003cul class=\"list\"\u003e\n\u003cli\u003eSodium propionate does not inhibit all competitors, and propionate-tolerant bacteria and fungi will grow.\u003c\/li\u003e\n\u003cli\u003eThe long incubation leaves plates vulnerable to drying and to overgrowth by a single fast isolate; pour full plates and read them more than once.\u003c\/li\u003e\n\u003cli\u003eCycloheximide and nystatin are heat-labile; adding them above 50 °C reduces their activity.\u003c\/li\u003e\n\u003cli\u003eCounts underestimate the actinomycete population in a sample, because the selective agents also restrict some target organisms.\u003c\/li\u003e\n\u003cli\u003eGlycerol must be added by the user. Preparing the powder alone gives a medium with no usable carbon source.\u003c\/li\u003e\n\u003cli\u003eGrowth rate and morphology vary widely between genera, so a single reading time will not suit every isolate.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eLiterature and References\u003c\/h3\u003e\n\u003col class=\"refs\"\u003e\n\u003cli\u003eAtlas RM. \u003cem\u003eHandbook of Microbiological Media\u003c\/em\u003e, 4th ed. CRC Press; 2010.\u003c\/li\u003e\n\u003cli\u003e\n\u003cem\u003eDifco \u0026amp; BBL Manual: Manual of Microbiological Culture Media\u003c\/em\u003e, 2nd ed. BD Diagnostics; 2009.\u003c\/li\u003e\n\u003cli\u003eWilliams ST, Davies FL. Use of antibiotics for selective isolation and enumeration of actinomycetes in soil. \u003cem\u003eJ Gen Microbiol\u003c\/em\u003e. 1965;38(2):251–261.\u003c\/li\u003e\n\u003cli\u003eGoodfellow M, Williams ST. Ecology of actinomycetes. \u003cem\u003eAnnu Rev Microbiol\u003c\/em\u003e. 1983;37:189–216.\u003c\/li\u003e\n\u003cli\u003eHayakawa M, Nonomura H. Humic acid-vitamin agar, a new medium for the selective isolation of soil actinomycetes. \u003cem\u003eJ Ferment Technol\u003c\/em\u003e. 1987;65(5):501–509.\u003c\/li\u003e\n\u003cli\u003eShirling EB, Gottlieb D. Methods for characterization of \u003cem\u003eStreptomyces\u003c\/em\u003e species. \u003cem\u003eInt J Syst Bacteriol\u003c\/em\u003e. 1966;16(3):313–340.\u003c\/li\u003e\n\u003c\/ol\u003e\u003c\/section\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003ePhysical Specifications\u003c\/h4\u003e\n\u003ctable class=\"specs\"\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance, powder\u003c\/td\u003e\n\u003ctd\u003eCream to light beige, homogeneous, free-flowing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance, prepared\u003c\/td\u003e\n\u003ctd\u003ePale straw, clear to slightly opalescent firm gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH (21.7 g\/L plus glycerol, 25 °C)\u003c\/td\u003e\n\u003ctd\u003e7.90–8.30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLoss on drying\u003c\/td\u003e\n\u003ctd\u003e≤ 6.0% w\/w\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGel strength\u003c\/td\u003e\n\u003ctd\u003eFirm, comparable with a 1.5% agar gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-green\"\u003e\n\u003ch4\u003eSupplements (not supplied)\u003c\/h4\u003e\n\u003cp\u003eGlycerol 5 mL\/L, added before autoclaving.\u003cbr\u003eCycloheximide 50 mg\/L, filter-sterilised, added after autoclaving.\u003cbr\u003eNystatin 50 mg\/L, filter-sterilised, optional for heavy fungal contamination.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eStorage and Stability\u003c\/h4\u003e\n\u003cp\u003eStore powder at 10–30 °C, tightly closed, dry and protected from light. Do not use if caked or discoloured. Prepared plates: 2–8 °C in sealed bags to limit moisture loss; shelf life to be established by the user.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003cdiv class=\"panel p3\"\u003e\u003cdiv class=\"grid\"\u003e\n\u003cdiv\u003e\n\u003csection\u003e\u003ch3\u003eHazard Identification\u003c\/h3\u003e\n\u003cdiv class=\"info\"\u003e\u003cstrong\u003eNot classified as hazardous according to the GHS (Rev. 7) as adopted in Australia under the model WHS Regulations. Not Dangerous Goods under the ADG Code.\u003c\/strong\u003e\u003c\/div\u003e\n\u003cp\u003eFine powder can form combustible dust concentrations in air. Dust may cause mild mechanical irritation of the eyes and respiratory tract. Contains casein-derived material: people with milk protein allergy should avoid exposure.\u003c\/p\u003e\n\u003cdiv class=\"warn\"\u003e\n\u003cstrong\u003eSupplements added by the user are hazardous and are not covered by this section.\u003c\/strong\u003e Cycloheximide is acutely toxic and a reproductive toxicant. Nystatin is a sensitiser. Consult the supplier's SDS for each supplement and handle it according to that document before adding it to this medium.\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eComposition\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredient\u003c\/th\u003e\n\u003cth\u003eCAS No.\u003c\/th\u003e\n\u003cth\u003e% w\/w\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e9002-18-0\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e69.12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium propionate\u003c\/td\u003e\n\u003ctd\u003e137-40-6\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e18.43\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium caseinate\u003c\/td\u003e\n\u003ctd\u003e9005-46-3\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e9.22\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDipotassium hydrogen phosphate\u003c\/td\u003e\n\u003ctd\u003e7758-11-4\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e2.30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Asparagine\u003c\/td\u003e\n\u003ctd\u003e70-47-3\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e0.46\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnesium sulfate heptahydrate\u003c\/td\u003e\n\u003ctd\u003e10034-99-8\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e0.46\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFerrous sulfate heptahydrate\u003c\/td\u003e\n\u003ctd\u003e7782-63-0\u003c\/td\u003e\n\u003ctd class=\"val\"\u003e0.01\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp class=\"small\"\u003ePercentages are nominal and calculated from the formulation.\u003c\/p\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eFirst Aid\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eRoute\u003c\/th\u003e\n\u003cth\u003eAction\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInhalation\u003c\/td\u003e\n\u003ctd\u003eMove to fresh air. Seek medical advice if symptoms persist.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSkin\u003c\/td\u003e\n\u003ctd\u003eWash with soap and water.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEyes\u003c\/td\u003e\n\u003ctd\u003eRinse with water for several minutes. Seek medical advice if irritation persists.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIngestion\u003c\/td\u003e\n\u003ctd\u003eRinse mouth. Do not induce vomiting. Seek medical advice if unwell.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cdiv class=\"warn\"\u003e\u003cstrong\u003ePoisons Information Centre (Australia, 24 h): 13 11 26\u003c\/strong\u003e\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003eHandling, Spills and Disposal\u003c\/h3\u003e\n\u003cul class=\"list\"\u003e\n\u003cli\u003eAvoid generating and breathing dust. Weigh in a ventilated area.\u003c\/li\u003e\n\u003cli\u003eSpills: sweep up or HEPA-vacuum without raising dust; wash the area with water.\u003c\/li\u003e\n\u003cli\u003eUnused product: dispose of as non-hazardous waste under local regulations.\u003c\/li\u003e\n\u003cli\u003eInoculated medium: treat as potentially biohazardous and decontaminate (for example by autoclaving) before disposal.\u003c\/li\u003e\n\u003cli\u003eMedium containing cycloheximide or nystatin: dispose of as chemical waste under local regulations, not as general waste.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c\/section\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003ePersonal Protection\u003c\/h4\u003e\n\u003ctable class=\"specs\"\u003e\n\u003ctr\u003e\n\u003ctd\u003eEyes\u003c\/td\u003e\n\u003ctd\u003eSafety glasses with side shields\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHands\u003c\/td\u003e\n\u003ctd\u003eNitrile gloves\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBody\u003c\/td\u003e\n\u003ctd\u003eLaboratory coat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRespiratory\u003c\/td\u003e\n\u003ctd\u003eP2 respirator if dust is generated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-green\"\u003e\n\u003ch4\u003eFire and Stability\u003c\/h4\u003e\n\u003cp\u003eCombustible solid. Extinguish with water spray, foam, dry powder or CO\u003csub\u003e2\u003c\/sub\u003e. Stable under recommended storage. Avoid moisture, heat and strong oxidising agents.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-gold\"\u003e\n\u003ch4\u003eTransport\u003c\/h4\u003e\n\u003cp\u003eNot regulated for road, rail, sea or air transport. No UN number or Hazchem code.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eFull SDS\u003c\/h4\u003e\n\u003cp\u003eThe complete 16-section SDS is available on request from \u003ca href=\"mailto:support@ausamics.com?subject=SDS%20request%20AS-1102\"\u003esupport@ausamics.com\u003c\/a\u003e.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003cdiv class=\"panel p4\"\u003e\u003cdiv class=\"grid\"\u003e\n\u003cdiv\u003e\n\u003csection\u003e\u003ch3\u003eQuality Control Specification\u003c\/h3\u003e\n\u003cdiv class=\"qc\"\u003e\n\u003cp style=\"font-size:14px\"\u003eEach batch is tested for growth performance. Prepared with glycerol, incubated aerobically at 28 ± 2 °C and read at 7 days.\u003c\/p\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable class=\"dark\"\u003e\n\u003ctr\u003e\n\u003cth\u003eQC organism\u003c\/th\u003e\n\u003cth\u003eStrain\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eExpected result\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cem\u003eStreptomyces griseus\u003c\/em\u003e\u003c\/td\u003e\n\u003ctd\u003eATCC 10137\u003c\/td\u003e\n\u003ctd\u003e≤ 100\u003c\/td\u003e\n\u003ctd\u003eGood growth; typical colonies with aerial mycelium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cem\u003eEscherichia coli\u003c\/em\u003e\u003c\/td\u003e\n\u003ctd\u003eATCC 25922\u003c\/td\u003e\n\u003ctd\u003e≤ 100\u003c\/td\u003e\n\u003ctd\u003ePartial to complete inhibition\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\n\u003cp class=\"small\" style=\"margin:10px 0 0;color:#2d5a3d\"\u003eSterility of the prepared medium: no growth after 14 days at 30–35 °C.\u003c\/p\u003e\n\u003c\/div\u003e\u003c\/section\u003e\n\u003csection\u003e\u003ch3\u003ePhysical and Chemical Tests\u003c\/h3\u003e\n\u003cdiv class=\"tw\"\u003e\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eTest\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance, powder\u003c\/td\u003e\n\u003ctd\u003eCream to light beige, homogeneous, free-flowing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance, prepared medium\u003c\/td\u003e\n\u003ctd\u003ePale straw, clear to slightly opalescent firm gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGel strength\u003c\/td\u003e\n\u003ctd\u003eFirm, comparable with a 1.5% agar gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH, prepared medium (25 °C)\u003c\/td\u003e\n\u003ctd\u003e7.90–8.30\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLoss on drying\u003c\/td\u003e\n\u003ctd\u003e≤ 6.0% w\/w\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSterility, prepared medium\u003c\/td\u003e\n\u003ctd\u003eNo growth at 14 days, 30–35 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003c\/div\u003e\u003c\/section\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv class=\"box s-gold\"\u003e\n\u003ch4\u003eCertificate of Analysis\u003c\/h4\u003e\n\u003cp style=\"margin-bottom:10px\"\u003eA batch-specific COA is available on request for every lot. It reports the lot number, manufacturing and expiry dates, and the results of the tests listed on this page.\u003c\/p\u003e\n\u003cp\u003eTo request a COA for a specific lot, email \u003ca href=\"mailto:support@ausamics.com?subject=COA%20request%20AS-1102\"\u003esupport@ausamics.com\u003c\/a\u003e with the lot number from your label.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"box s-white\"\u003e\n\u003ch4\u003eManufacturing\u003c\/h4\u003e\n\u003cp\u003eManufactured by Ausamics Pty Ltd in Melbourne, Australia.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"foot\"\u003eThe information on this page is provided in good faith as a guide to the properties and use of this product and does not constitute a warranty of fitness for a particular purpose. Users are responsible for confirming suitability for their application. Ausamics Pty Ltd · ABN 56 676 640 467 · 36\/25 Trafalgar Road, Epping VIC 3076, Australia · support@ausamics.com\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Ausamics Life Science","offers":[{"title":"100 g","offer_id":48044513788159,"sku":"AS-1102-100G","price":45.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":48044513820927,"sku":"AS-1102-500G","price":97.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/Actinomycet_Agar_AC_Agar.jpg?v=1784950850","url":"https:\/\/ausamics.com.au\/products\/actinomycete-isolation-agar","provider":"AuSaMicS","version":"1.0","type":"link"}