{"product_id":"antibiotic-medium-11-neomycin-assay-agar","title":"Antibiotic Medium No. 11","description":"\u003ch3 data-start=\"265\" data-end=\"295\"\u003eAntibiotic Medium No. 11\u003c\/h3\u003e\n\u003cp data-start=\"296\" data-end=\"362\"\u003eOfficial Seed Agar for Neomycin \u0026amp; Framycetin Cylinder-Plate Assays\u003c\/p\u003e\n\u003cp data-start=\"364\" data-end=\"393\"\u003e\u003cstrong data-start=\"364\" data-end=\"383\"\u003eCatalog Number:\u003c\/strong\u003e AS-1109\u003c\/p\u003e\n\u003ch4 data-start=\"395\" data-end=\"410\"\u003eOverview\u003c\/h4\u003e\n\u003cp data-start=\"411\" data-end=\"773\"\u003eAntibiotic Medium No. 11 is the official standardized \u003cstrong data-start=\"465\" data-end=\"478\"\u003eseed agar\u003c\/strong\u003e developed specifically for the microbiological \u003cstrong data-start=\"526\" data-end=\"562\"\u003ecylinder-plate (cup-plate) assay\u003c\/strong\u003e of neomycin, framycetin, and related aminoglycoside antibiotics. It is designated as \u003cstrong data-start=\"648\" data-end=\"662\"\u003e“Medium E”\u003c\/strong\u003e in \u003cstrong data-start=\"666\" data-end=\"678\"\u003eUSP \u0026lt;81\u0026gt;\u003c\/strong\u003e, \u003cstrong data-start=\"680\" data-end=\"712\"\u003eEuropean Pharmacopoeia 2.7.2\u003c\/strong\u003e, \u003cstrong data-start=\"714\" data-end=\"748\"\u003eJapanese Pharmacopoeia (JP 17)\u003c\/strong\u003e, and \u003cstrong data-start=\"754\" data-end=\"761\"\u003eWHO\u003c\/strong\u003e guidelines.\u003c\/p\u003e\n\u003cp data-start=\"775\" data-end=\"1131\"\u003eThe formulation is carefully adjusted to a \u003cstrong data-start=\"818\" data-end=\"852\"\u003eslightly alkaline pH (7.9–8.1)\u003c\/strong\u003e, which is essential for optimal aminoglycoside diffusion. When seeded with the appropriate test organism, this medium produces \u003cstrong data-start=\"980\" data-end=\"1052\"\u003eexceptionally sharp, large, and highly reproducible inhibition zones\u003c\/strong\u003e, making it the global reference medium for regulatory neomycin potency assays.\u003c\/p\u003e\n\u003ch4 data-start=\"1133\" data-end=\"1163\"\u003eKey Features \u0026amp; Benefits\u003c\/h4\u003e\n\u003cp data-start=\"1164\" data-end=\"1227\"\u003eSlightly alkaline pH (≈ 8.0) optimised for neomycin diffusion\u003c\/p\u003e\n\u003cp data-start=\"1229\" data-end=\"1291\"\u003eInternationally harmonised formulation (USP \/ EP \/ JP \/ WHO)\u003c\/p\u003e\n\u003cp data-start=\"1293\" data-end=\"1372\"\u003eProduces the clearest and largest inhibition zones among neomycin assay media\u003c\/p\u003e\n\u003cp data-start=\"1374\" data-end=\"1418\"\u003eHigh assay sensitivity and reproducibility\u003c\/p\u003e\n\u003cp data-start=\"1420\" data-end=\"1473\"\u003eOfficial reference seed agar for regulatory testing\u003c\/p\u003e\n\u003ch4 data-start=\"1475\" data-end=\"1502\"\u003eTypical Applications\u003c\/h4\u003e\n\u003cp data-start=\"1504\" data-end=\"1587\"\u003e\u003cstrong data-start=\"1504\" data-end=\"1538\"\u003ePharmaceutical Quality Control\u003c\/strong\u003e\u003cbr data-start=\"1538\" data-end=\"1541\"\u003eCompendial potency assay of neomycin sulfate\u003c\/p\u003e\n\u003cp data-start=\"1589\" data-end=\"1637\"\u003eCompendial potency assay of framycetin sulfate\u003c\/p\u003e\n\u003cp data-start=\"1639\" data-end=\"1689\"\u003eRoutine QC testing of aminoglycoside antibiotics\u003c\/p\u003e\n\u003cp data-start=\"1691\" data-end=\"1779\"\u003e\u003cstrong data-start=\"1691\" data-end=\"1726\"\u003eCylinder-Plate Diffusion Assays\u003c\/strong\u003e\u003cbr data-start=\"1726\" data-end=\"1729\"\u003eAssay of neomycin-containing ophthalmic products\u003c\/p\u003e\n\u003cp data-start=\"1781\" data-end=\"1836\"\u003eAssay of topical and oral pharmaceutical formulations\u003c\/p\u003e\n\u003cp data-start=\"1838\" data-end=\"1880\"\u003eBioequivalence and batch-release testing\u003c\/p\u003e\n\u003ch4 data-start=\"1882\" data-end=\"1912\"\u003ePrinciple of the Medium\u003c\/h4\u003e\n\u003cp data-start=\"1913\" data-end=\"2255\"\u003eAntibiotic Medium No. 11 provides an optimised nutrient base and alkaline environment that enhances aminoglycoside diffusion through agar. When inoculated with a sensitive test organism, neomycin and framycetin diffuse uniformly from cylinders or cups, producing inhibition zones whose diameter is directly proportional to antibiotic potency.\u003c\/p\u003e\n\u003ch4 data-start=\"2257\" data-end=\"2295\"\u003eTypical Composition (per litre)\u003c\/h4\u003e\n\u003cp data-start=\"2296\" data-end=\"2330\"\u003ePeptone (meat or casein) – 6.0 g\u003c\/p\u003e\n\u003cp data-start=\"2332\" data-end=\"2354\"\u003eBeef Extract – 1.5 g\u003c\/p\u003e\n\u003cp data-start=\"2356\" data-end=\"2379\"\u003eYeast Extract – 3.0 g\u003c\/p\u003e\n\u003cp data-start=\"2381\" data-end=\"2400\"\u003eD-Glucose – 1.0 g\u003c\/p\u003e\n\u003cp data-start=\"2402\" data-end=\"2417\"\u003eAgar – 15.0 g\u003c\/p\u003e\n\u003cp data-start=\"2419\" data-end=\"2475\"\u003eFinal pH: \u003cstrong data-start=\"2429\" data-end=\"2473\"\u003e7.9 – 8.1 at 25 °C (after sterilisation)\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch4 data-start=\"2477\" data-end=\"2495\"\u003ePreparation\u003c\/h4\u003e\n\u003cp data-start=\"2496\" data-end=\"2555\"\u003eSuspend 26.5 g of dehydrated medium in 1 L purified water\u003c\/p\u003e\n\u003cp data-start=\"2557\" data-end=\"2639\"\u003eHeat with frequent agitation and boil for 1–2 minutes until completely dissolved\u003c\/p\u003e\n\u003cp data-start=\"2641\" data-end=\"2692\"\u003eSterilise by autoclaving at 121 °C for 15 minutes\u003c\/p\u003e\n\u003cp data-start=\"2694\" data-end=\"2768\"\u003eAfter sterilisation, verify pH is \u003cstrong data-start=\"2728\" data-end=\"2741\"\u003e8.0 ± 0.1\u003c\/strong\u003e (critical specification)\u003c\/p\u003e\n\u003cp data-start=\"2770\" data-end=\"2788\"\u003eCool to 48–50 °C\u003c\/p\u003e\n\u003cp data-start=\"2790\" data-end=\"2836\"\u003eInoculate with a standardised suspension of:\u003c\/p\u003e\n\u003cul data-start=\"2837\" data-end=\"2935\"\u003e\n\u003cli data-start=\"2837\" data-end=\"2896\"\u003e\n\u003cp data-start=\"2839\" data-end=\"2896\"\u003e\u003cem data-start=\"2839\" data-end=\"2867\"\u003eStaphylococcus epidermidis\u003c\/em\u003e ATCC 12228 (preferred), or\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2897\" data-end=\"2935\"\u003e\n\u003cp data-start=\"2899\" data-end=\"2935\"\u003e\u003cem data-start=\"2899\" data-end=\"2922\"\u003eKlebsiella pneumoniae\u003c\/em\u003e ATCC 10031\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2937\" data-end=\"3047\"\u003ePour 4–6 mL of seeded medium over a previously prepared base layer\u003cbr data-start=\"3003\" data-end=\"3006\"\u003e(typically \u003cstrong data-start=\"3017\" data-end=\"3044\"\u003eAntibiotic Medium No. 2\u003c\/strong\u003e)\u003c\/p\u003e\n\u003ch4 data-start=\"3049\" data-end=\"3075\"\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cp data-start=\"3076\" data-end=\"3135\"\u003eDehydrated medium: Store below 30 °C in a cool, dry place\u003c\/p\u003e\n\u003cp data-start=\"3137\" data-end=\"3182\"\u003ePrepared seeded plates: Use within 24 hours\u003c\/p\u003e\n\u003cp data-start=\"3184\" data-end=\"3237\"\u003eIf required, store at 2–8 °C for short periods only\u003c\/p\u003e\n\u003ch4 data-start=\"3239\" data-end=\"3258\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp data-start=\"3259\" data-end=\"3397\"\u003eFor pharmaceutical quality-control and official compendial antibiotic assays only.\u003cbr data-start=\"3341\" data-end=\"3344\"\u003eNot for clinical diagnostic, food, or veterinary use.\u003c\/p\u003e\n\u003ch4 data-start=\"3399\" data-end=\"3426\"\u003eQuality \u0026amp; Compliance\u003c\/h4\u003e\n\u003cp data-start=\"3427\" data-end=\"3490\"\u003eComplies with USP \u0026lt;81\u0026gt;, EP 2.7.2, JP 17, and WHO requirements\u003c\/p\u003e\n\u003cp data-start=\"3492\" data-end=\"3565\"\u003eManufactured under controlled conditions for batch-to-batch consistency\u003c\/p\u003e\n\u003cp data-start=\"3567\" data-end=\"3619\"\u003eSuitable for regulated pharmaceutical laboratories\u003c\/p\u003e\n\u003ch4 data-start=\"3621\" data-end=\"3655\"\u003eCustoms \u0026amp; Trade Information\u003c\/h4\u003e\n\u003cp data-start=\"3656\" data-end=\"3763\"\u003eHS \/ AHECC Code: \u003cstrong data-start=\"3673\" data-end=\"3687\"\u003e3821.00.00\u003c\/strong\u003e\u003cbr data-start=\"3687\" data-end=\"3690\"\u003ePrepared culture media for development or maintenance of microorganisms\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"100G","offer_id":48051914178815,"sku":"AS-1112-DH-LS-100G","price":55.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_5793101b-1d71-40c0-b95f-b078e2b5c850.jpg?v=1771063034","url":"https:\/\/ausamics.com.au\/products\/antibiotic-medium-11-neomycin-assay-agar","provider":"AuSaMicS","version":"1.0","type":"link"}