{"product_id":"antibiotic-medium-no-10-seed-agar-for-polymyxin-assays","title":"Antibiotic Medium No. 10","description":"\u003ch3 data-start=\"211\" data-end=\"241\"\u003eAntibiotic Medium No. 10\u003c\/h3\u003e\n\u003cp data-start=\"242\" data-end=\"309\"\u003eOfficial Seed Agar for Polymyxin B \u0026amp; Colistin Cylinder-Plate Assays\u003c\/p\u003e\n\u003cp data-start=\"311\" data-end=\"340\"\u003e\u003cstrong data-start=\"311\" data-end=\"330\"\u003eCatalog Number:\u003c\/strong\u003e AS-1111\u003c\/p\u003e\n\u003ch4 data-start=\"342\" data-end=\"357\"\u003eOverview\u003c\/h4\u003e\n\u003cp data-start=\"358\" data-end=\"682\"\u003eAntibiotic Medium No. 10 is a specialised \u003cstrong data-start=\"400\" data-end=\"413\"\u003eseed agar\u003c\/strong\u003e formulated for the microbiological \u003cstrong data-start=\"449\" data-end=\"485\"\u003ecylinder-plate (cup-plate) assay\u003c\/strong\u003e of \u003cstrong data-start=\"489\" data-end=\"504\"\u003epolymyxin B\u003c\/strong\u003e and \u003cstrong data-start=\"509\" data-end=\"535\"\u003ecolistin (polymyxin E)\u003c\/strong\u003e. It is designated as \u003cstrong data-start=\"557\" data-end=\"571\"\u003e“Medium K”\u003c\/strong\u003e in \u003cstrong data-start=\"575\" data-end=\"587\"\u003eUSP \u0026lt;81\u0026gt;\u003c\/strong\u003e, \u003cstrong data-start=\"589\" data-end=\"621\"\u003eEuropean Pharmacopoeia 2.7.2\u003c\/strong\u003e, \u003cstrong data-start=\"623\" data-end=\"657\"\u003eJapanese Pharmacopoeia (JP 17)\u003c\/strong\u003e, and \u003cstrong data-start=\"663\" data-end=\"670\"\u003eWHO\u003c\/strong\u003e guidelines.\u003c\/p\u003e\n\u003cp data-start=\"684\" data-end=\"1059\"\u003eThe formulation uniquely contains \u003cstrong data-start=\"718\" data-end=\"747\"\u003emanganese chloride (Mn²⁺)\u003c\/strong\u003e, an essential component for the optimal growth, metabolic activity, and antibiotic sensitivity of the required test organism, \u003cem data-start=\"874\" data-end=\"901\"\u003eBordetella bronchiseptica\u003c\/em\u003e. The presence of Mn²⁺ enables the formation of \u003cstrong data-start=\"949\" data-end=\"1007\"\u003elarge, sharp, and highly reproducible inhibition zones\u003c\/strong\u003e, which are characteristic of polymyxin antibiotics.\u003c\/p\u003e\n\u003cp data-start=\"1061\" data-end=\"1280\"\u003eAntibiotic Medium No. 10 is manufactured to deliver \u003cstrong data-start=\"1113\" data-end=\"1139\"\u003ecompendial performance\u003c\/strong\u003e, consistent organism response, and high assay reproducibility, making it suitable for regulated pharmaceutical quality-control laboratories.\u003c\/p\u003e\n\u003ch4 data-start=\"1282\" data-end=\"1312\"\u003eKey Features \u0026amp; Benefits\u003c\/h4\u003e\n\u003cp data-start=\"1313\" data-end=\"1396\"\u003eContains Mn²⁺ essential for \u003cem data-start=\"1341\" data-end=\"1368\"\u003eBordetella bronchiseptica\u003c\/em\u003e sensitivity to polymyxins\u003c\/p\u003e\n\u003cp data-start=\"1398\" data-end=\"1462\"\u003eProduces clear, wide, and highly reproducible inhibition zones\u003c\/p\u003e\n\u003cp data-start=\"1464\" data-end=\"1519\"\u003eGlobally harmonised formulation (USP \/ EP \/ JP \/ WHO)\u003c\/p\u003e\n\u003cp data-start=\"1521\" data-end=\"1574\"\u003eOfficial reference seed medium for polymyxin assays\u003c\/p\u003e\n\u003cp data-start=\"1576\" data-end=\"1643\"\u003eOptimised for consistent organism growth and antibiotic diffusion\u003c\/p\u003e\n\u003ch4 data-start=\"1645\" data-end=\"1672\"\u003eTypical Applications\u003c\/h4\u003e\n\u003cp data-start=\"1674\" data-end=\"1745\"\u003e\u003cstrong data-start=\"1674\" data-end=\"1704\"\u003eOfficial Compendial Assays\u003c\/strong\u003e\u003cbr data-start=\"1704\" data-end=\"1707\"\u003ePotency assay of polymyxin B sulfate\u003c\/p\u003e\n\u003cp data-start=\"1747\" data-end=\"1796\"\u003ePotency assay of colistin sulfate (polymyxin E)\u003c\/p\u003e\n\u003cp data-start=\"1798\" data-end=\"1891\"\u003e\u003cstrong data-start=\"1798\" data-end=\"1833\"\u003eCylinder-Plate Diffusion Assays\u003c\/strong\u003e\u003cbr data-start=\"1833\" data-end=\"1836\"\u003eAssay of polymyxin-containing pharmaceutical products\u003c\/p\u003e\n\u003cp data-start=\"1893\" data-end=\"1973\"\u003e\u003cstrong data-start=\"1893\" data-end=\"1927\"\u003ePharmaceutical Quality Control\u003c\/strong\u003e\u003cbr data-start=\"1927\" data-end=\"1930\"\u003eTesting of topical polymyxin formulations\u003c\/p\u003e\n\u003cp data-start=\"1975\" data-end=\"2021\"\u003eTesting of ophthalmic polymyxin preparations\u003c\/p\u003e\n\u003cp data-start=\"2023\" data-end=\"2065\"\u003eTesting of parenteral polymyxin products\u003c\/p\u003e\n\u003ch4 data-start=\"2067\" data-end=\"2097\"\u003ePrinciple of the Medium\u003c\/h4\u003e\n\u003cp data-start=\"2098\" data-end=\"2458\"\u003eAntibiotic Medium No. 10 provides a nutrient-balanced agar matrix supplemented with manganese ions, which are required for optimal metabolic activity and antibiotic response of \u003cem data-start=\"2275\" data-end=\"2302\"\u003eBordetella bronchiseptica\u003c\/em\u003e. This formulation ensures stable organism growth, controlled diffusion of polymyxins, and accurate, reproducible zone measurement in cylinder-plate assays.\u003c\/p\u003e\n\u003ch4 data-start=\"2460\" data-end=\"2498\"\u003eTypical Composition (per litre)\u003c\/h4\u003e\n\u003cp data-start=\"2499\" data-end=\"2533\"\u003ePeptone (meat or casein) – 6.0 g\u003c\/p\u003e\n\u003cp data-start=\"2535\" data-end=\"2557\"\u003eBeef Extract – 1.5 g\u003c\/p\u003e\n\u003cp data-start=\"2559\" data-end=\"2582\"\u003eYeast Extract – 3.0 g\u003c\/p\u003e\n\u003cp data-start=\"2584\" data-end=\"2603\"\u003eD-Glucose – 1.0 g\u003c\/p\u003e\n\u003cp data-start=\"2605\" data-end=\"2665\"\u003eManganese Chloride (MnCl₂·4H₂O) – 0.097 g\u003cbr data-start=\"2646\" data-end=\"2649\"\u003e(≈ 27 mg Mn²⁺)\u003c\/p\u003e\n\u003cp data-start=\"2667\" data-end=\"2682\"\u003eAgar – 15.0 g\u003c\/p\u003e\n\u003cp data-start=\"2684\" data-end=\"2740\"\u003eFinal pH: \u003cstrong data-start=\"2694\" data-end=\"2738\"\u003e7.2 ± 0.1 at 25 °C (after sterilisation)\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch4 data-start=\"2742\" data-end=\"2760\"\u003ePreparation\u003c\/h4\u003e\n\u003cp data-start=\"2761\" data-end=\"2822\"\u003eSuspend 26.597 g of dehydrated medium in 1 L purified water\u003c\/p\u003e\n\u003cp data-start=\"2824\" data-end=\"2901\"\u003eHeat with frequent agitation and boil for 1–2 minutes until fully dissolved\u003c\/p\u003e\n\u003cp data-start=\"2903\" data-end=\"2954\"\u003eSterilise by autoclaving at 121 °C for 15 minutes\u003c\/p\u003e\n\u003cp data-start=\"2956\" data-end=\"2974\"\u003eCool to 48–50 °C\u003c\/p\u003e\n\u003cp data-start=\"2976\" data-end=\"3059\"\u003eInoculate with a standardised suspension of \u003cem data-start=\"3020\" data-end=\"3047\"\u003eBordetella bronchiseptica\u003c\/em\u003e ATCC 4617\u003c\/p\u003e\n\u003cp data-start=\"3061\" data-end=\"3126\"\u003ePour immediately as the \u003cstrong data-start=\"3085\" data-end=\"3099\"\u003eseed layer\u003c\/strong\u003e in cylinder-plate assays\u003c\/p\u003e\n\u003ch4 data-start=\"3128\" data-end=\"3157\"\u003eRequired Test Organism\u003c\/h4\u003e\n\u003cp data-start=\"3158\" data-end=\"3197\"\u003e\u003cem data-start=\"3158\" data-end=\"3185\"\u003eBordetella bronchiseptica\u003c\/em\u003e ATCC 4617\u003c\/p\u003e\n\u003cp data-start=\"3199\" data-end=\"3242\"\u003eEquivalent strains: NCIMB 8344, NCTC 8344\u003c\/p\u003e\n\u003cp data-start=\"3244\" data-end=\"3303\"\u003eMandatory test organism for all official polymyxin assays\u003c\/p\u003e\n\u003ch4 data-start=\"3305\" data-end=\"3331\"\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cp data-start=\"3332\" data-end=\"3391\"\u003eDehydrated medium: Store below 30 °C in a cool, dry place\u003c\/p\u003e\n\u003cp data-start=\"3393\" data-end=\"3482\"\u003ePrepared seeded plates:\u003cbr data-start=\"3416\" data-end=\"3419\"\u003eUse immediately, or\u003cbr data-start=\"3438\" data-end=\"3441\"\u003eStore at 2–8 °C and use within 24 hours\u003c\/p\u003e\n\u003ch4 data-start=\"3484\" data-end=\"3503\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp data-start=\"3504\" data-end=\"3654\"\u003eFor pharmaceutical quality-control and official compendial antibiotic assay applications only.\u003cbr data-start=\"3598\" data-end=\"3601\"\u003eNot for clinical diagnostic, food, or veterinary use.\u003c\/p\u003e\n\u003ch4 data-start=\"3656\" data-end=\"3683\"\u003eQuality \u0026amp; Compliance\u003c\/h4\u003e\n\u003cp data-start=\"3684\" data-end=\"3738\"\u003eFormulated to meet USP, EP, JP, and WHO requirements\u003c\/p\u003e\n\u003cp data-start=\"3740\" data-end=\"3805\"\u003eSuitable for regulated pharmaceutical microbiology laboratories\u003c\/p\u003e\n\u003cp data-start=\"3807\" data-end=\"3872\"\u003ePerformance comparable to leading international reference media\u003c\/p\u003e\n\u003ch4 data-start=\"3874\" data-end=\"3908\"\u003eCustoms \u0026amp; Trade Information\u003c\/h4\u003e\n\u003cp data-start=\"3909\" data-end=\"4016\"\u003eHS \/ AHECC Code: \u003cstrong data-start=\"3926\" data-end=\"3940\"\u003e3821.00.00\u003c\/strong\u003e\u003cbr data-start=\"3940\" data-end=\"3943\"\u003ePrepared culture media for development or maintenance of microorganisms\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"100G","offer_id":48051960021247,"sku":"AS-1111-DH-LS-100G","price":45.0,"currency_code":"AUD","in_stock":true},{"title":"500G","offer_id":48051960054015,"sku":"AS-1111-DH-LS-500G","price":125.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_c7a34fba-b124-4e94-806d-de12fc30592f.jpg?v=1771063034","url":"https:\/\/ausamics.com.au\/products\/antibiotic-medium-no-10-seed-agar-for-polymyxin-assays","provider":"AuSaMicS","version":"1.0","type":"link"}