{"product_id":"antibiotic-medium-no-13-sabouraud-2-dextrose-broth","title":"Antibiotic Medium No. 13","description":"\u003ch3 data-start=\"168\" data-end=\"198\"\u003eAntibiotic Medium No. 13\u003c\/h3\u003e\n\u003cp data-start=\"199\" data-end=\"281\"\u003eOfficial Liquid Medium for Yeast Inoculum Preparation in Polyene Antifungal Assays\u003c\/p\u003e\n\u003cp data-start=\"283\" data-end=\"312\"\u003e\u003cstrong data-start=\"283\" data-end=\"302\"\u003eCatalog Number:\u003c\/strong\u003e AS-1113\u003c\/p\u003e\n\u003ch4 data-start=\"314\" data-end=\"329\"\u003eOverview\u003c\/h4\u003e\n\u003cp data-start=\"330\" data-end=\"695\"\u003eAntibiotic Medium No. 13 is the official \u003cstrong data-start=\"371\" data-end=\"388\"\u003eliquid medium\u003c\/strong\u003e used for the preparation of standardized yeast inoculum and spore suspensions in the \u003cstrong data-start=\"474\" data-end=\"509\"\u003ecompendial cylinder-plate assay\u003c\/strong\u003e of \u003cstrong data-start=\"513\" data-end=\"525\"\u003enystatin\u003c\/strong\u003e and \u003cstrong data-start=\"530\" data-end=\"548\"\u003eamphotericin B\u003c\/strong\u003e. It is designated as \u003cstrong data-start=\"570\" data-end=\"584\"\u003e“Medium N”\u003c\/strong\u003e in \u003cstrong data-start=\"588\" data-end=\"600\"\u003eUSP \u0026lt;81\u0026gt;\u003c\/strong\u003e, \u003cstrong data-start=\"602\" data-end=\"634\"\u003eEuropean Pharmacopoeia 2.7.2\u003c\/strong\u003e, \u003cstrong data-start=\"636\" data-end=\"670\"\u003eJapanese Pharmacopoeia (JP 17)\u003c\/strong\u003e, and \u003cstrong data-start=\"676\" data-end=\"683\"\u003eWHO\u003c\/strong\u003e guidelines.\u003c\/p\u003e\n\u003cp data-start=\"697\" data-end=\"1008\"\u003eThe medium is based on the classic \u003cstrong data-start=\"732\" data-end=\"763\"\u003eSabouraud broth formulation\u003c\/strong\u003e, containing \u003cstrong data-start=\"776\" data-end=\"790\"\u003e2% glucose\u003c\/strong\u003e and a \u003cstrong data-start=\"797\" data-end=\"829\"\u003eslightly acidic pH (5.6–5.8)\u003c\/strong\u003e. This composition promotes rapid, heavy growth and excellent viability of yeast cells, ensuring consistent and reproducible preparation of inoculum for antifungal potency assays.\u003c\/p\u003e\n\u003ch4 data-start=\"1010\" data-end=\"1040\"\u003eKey Features \u0026amp; Benefits\u003c\/h4\u003e\n\u003cp data-start=\"1041\" data-end=\"1088\"\u003eClassic Sabouraud formulation with 2% glucose\u003c\/p\u003e\n\u003cp data-start=\"1090\" data-end=\"1164\"\u003eSlightly acidic pH (≈ 5.7) optimal for yeast growth and spore production\u003c\/p\u003e\n\u003cp data-start=\"1166\" data-end=\"1223\"\u003eInternationally harmonised medium (USP \/ EP \/ JP \/ WHO)\u003c\/p\u003e\n\u003cp data-start=\"1225\" data-end=\"1281\"\u003eOfficial reference broth for polyene antifungal assays\u003c\/p\u003e\n\u003cp data-start=\"1283\" data-end=\"1349\"\u003eEnsures reproducible inoculum preparation for seeded assay media\u003c\/p\u003e\n\u003ch4 data-start=\"1351\" data-end=\"1378\"\u003eTypical Applications\u003c\/h4\u003e\n\u003cp data-start=\"1380\" data-end=\"1488\"\u003e\u003cstrong data-start=\"1380\" data-end=\"1412\"\u003eCompendial Antifungal Assays\u003c\/strong\u003e\u003cbr data-start=\"1412\" data-end=\"1415\"\u003ePreparation of standardised yeast inoculum for nystatin potency testing\u003c\/p\u003e\n\u003cp data-start=\"1490\" data-end=\"1556\"\u003ePreparation of inoculum for amphotericin B cylinder-plate assays\u003c\/p\u003e\n\u003cp data-start=\"1558\" data-end=\"1638\"\u003e\u003cstrong data-start=\"1558\" data-end=\"1592\"\u003ePharmaceutical Quality Control\u003c\/strong\u003e\u003cbr data-start=\"1592\" data-end=\"1595\"\u003eGrowth and maintenance of assay organisms\u003c\/p\u003e\n\u003cp data-start=\"1640\" data-end=\"1691\"\u003eRoutine QC testing of polyene antifungal products\u003c\/p\u003e\n\u003cp data-start=\"1693\" data-end=\"1782\"\u003e\u003cstrong data-start=\"1693\" data-end=\"1733\"\u003eMicrobiological Inoculum Preparation\u003c\/strong\u003e\u003cbr data-start=\"1733\" data-end=\"1736\"\u003eCultivation of \u003cem data-start=\"1751\" data-end=\"1769\"\u003eCandida albicans\u003c\/em\u003e ATCC 10231\u003c\/p\u003e\n\u003cp data-start=\"1784\" data-end=\"1837\"\u003eCultivation of \u003cem data-start=\"1799\" data-end=\"1825\"\u003eSaccharomyces cerevisiae\u003c\/em\u003e ATCC 9763\u003c\/p\u003e\n\u003ch4 data-start=\"1839\" data-end=\"1869\"\u003ePrinciple of the Medium\u003c\/h4\u003e\n\u003cp data-start=\"1870\" data-end=\"2218\"\u003eAntibiotic Medium No. 13 provides a nutrient-rich, mildly acidic environment that supports rapid yeast proliferation and high spore viability. The resulting growth can be washed and diluted to obtain a standardised inoculum, which is then used to seed \u003cstrong data-start=\"2122\" data-end=\"2150\"\u003eAntibiotic Medium No. 12\u003c\/strong\u003e or other assay plates for polyene antifungal potency determination.\u003c\/p\u003e\n\u003ch4 data-start=\"2220\" data-end=\"2258\"\u003eTypical Composition (per litre)\u003c\/h4\u003e\n\u003cp data-start=\"2259\" data-end=\"2294\"\u003ePeptone (meat or casein) – 10.0 g\u003c\/p\u003e\n\u003cp data-start=\"2296\" data-end=\"2327\"\u003eD-Glucose (Dextrose) – 20.0 g\u003c\/p\u003e\n\u003cp data-start=\"2329\" data-end=\"2385\"\u003eFinal pH: \u003cstrong data-start=\"2339\" data-end=\"2383\"\u003e5.7 ± 0.1 at 25 °C (after sterilisation)\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch4 data-start=\"2387\" data-end=\"2405\"\u003ePreparation\u003c\/h4\u003e\n\u003cp data-start=\"2406\" data-end=\"2472\"\u003eDissolve 10.0 g peptone and 20.0 g glucose in 1 L purified water\u003c\/p\u003e\n\u003cp data-start=\"2474\" data-end=\"2517\"\u003eMix thoroughly until completely dissolved\u003c\/p\u003e\n\u003cp data-start=\"2519\" data-end=\"2562\"\u003eDispense into tubes or flasks as required\u003c\/p\u003e\n\u003cp data-start=\"2564\" data-end=\"2615\"\u003eSterilise by autoclaving at 121 °C for 15 minutes\u003c\/p\u003e\n\u003cp data-start=\"2617\" data-end=\"2654\"\u003eCool to room temperature before use\u003c\/p\u003e\n\u003ch4 data-start=\"2656\" data-end=\"2677\"\u003eUsage in Assay\u003c\/h4\u003e\n\u003cp data-start=\"2678\" data-end=\"2714\"\u003eInoculate with a fresh culture of:\u003c\/p\u003e\n\u003cul data-start=\"2715\" data-end=\"2793\"\u003e\n\u003cli data-start=\"2715\" data-end=\"2752\"\u003e\n\u003cp data-start=\"2717\" data-end=\"2752\"\u003e\u003cem data-start=\"2717\" data-end=\"2735\"\u003eCandida albicans\u003c\/em\u003e ATCC 10231, or\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2753\" data-end=\"2793\"\u003e\n\u003cp data-start=\"2755\" data-end=\"2793\"\u003e\u003cem data-start=\"2755\" data-end=\"2781\"\u003eSaccharomyces cerevisiae\u003c\/em\u003e ATCC 9763\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2795\" data-end=\"2833\"\u003eIncubate at 30–35 °C for 24–48 hours\u003c\/p\u003e\n\u003cp data-start=\"2835\" data-end=\"2924\"\u003eHarvest, wash, and dilute the growth to prepare a standardised spore or cell suspension\u003c\/p\u003e\n\u003cp data-start=\"2926\" data-end=\"3014\"\u003eUse the suspension to seed \u003cstrong data-start=\"2953\" data-end=\"2981\"\u003eAntibiotic Medium No. 12\u003c\/strong\u003e or other validated assay media\u003c\/p\u003e\n\u003ch4 data-start=\"3016\" data-end=\"3042\"\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cp data-start=\"3043\" data-end=\"3133\"\u003eDehydrated components or prepared broth: Store at room temperature, protected from light\u003c\/p\u003e\n\u003cp data-start=\"3135\" data-end=\"3189\"\u003ePrepared medium: Stable for up to 3 months at 2–8 °C\u003c\/p\u003e\n\u003ch4 data-start=\"3191\" data-end=\"3210\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp data-start=\"3211\" data-end=\"3349\"\u003eFor pharmaceutical quality-control and official compendial antifungal assays only.\u003cbr data-start=\"3293\" data-end=\"3296\"\u003eNot for clinical diagnostic, food, or veterinary use.\u003c\/p\u003e\n\u003ch4 data-start=\"3351\" data-end=\"3378\"\u003eQuality \u0026amp; Compliance\u003c\/h4\u003e\n\u003cp data-start=\"3379\" data-end=\"3447\"\u003eFormulated to meet USP \u0026lt;81\u0026gt;, EP 2.7.2, JP 17, and WHO requirements\u003c\/p\u003e\n\u003cp data-start=\"3449\" data-end=\"3522\"\u003eManufactured under controlled conditions for batch-to-batch consistency\u003c\/p\u003e\n\u003cp data-start=\"3524\" data-end=\"3589\"\u003eSuitable for regulated pharmaceutical microbiology laboratories\u003c\/p\u003e\n\u003ch4 data-start=\"3591\" data-end=\"3625\"\u003eCustoms \u0026amp; Trade Information\u003c\/h4\u003e\n\u003cp data-start=\"3626\" data-end=\"3733\"\u003eHS \/ AHECC Code: \u003cstrong data-start=\"3643\" data-end=\"3657\"\u003e3821.00.00\u003c\/strong\u003e\u003cbr data-start=\"3657\" data-end=\"3660\"\u003ePrepared culture media for development or maintenance of microorganisms\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"100G","offer_id":48051983646975,"sku":"AS-1115-DH-LS-100G","price":55.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_10851f69-0700-49c1-9c8a-d1dfe8e1eaed.jpg?v=1771063032","url":"https:\/\/ausamics.com.au\/products\/antibiotic-medium-no-13-sabouraud-2-dextrose-broth","provider":"AuSaMicS","version":"1.0","type":"link"}