{"product_id":"antibiotic-medium-no-2-penassay-base-agar","title":"Antibiotic Medium No. 2","description":"\u003ch3 data-start=\"356\" data-end=\"406\"\u003eAntibiotic Medium No. 2 (Penassay Base Agar)\u003c\/h3\u003e\n\u003cp data-start=\"407\" data-end=\"473\"\u003eBase-Layer Agar for Cylinder-Plate (Cup-Plate) Antibiotic Assays\u003c\/p\u003e\n\u003cp data-start=\"475\" data-end=\"504\"\u003e\u003cstrong data-start=\"475\" data-end=\"494\"\u003eCatalog Number:\u003c\/strong\u003e AS-1109\u003c\/p\u003e\n\u003ch4 data-start=\"506\" data-end=\"521\"\u003eOverview\u003c\/h4\u003e\n\u003cp data-start=\"522\" data-end=\"964\"\u003eAntibiotic Medium No. 2 is the official standardized \u003cstrong data-start=\"575\" data-end=\"594\"\u003ebase-layer agar\u003c\/strong\u003e used in the cylinder-plate (cup-plate) microbiological assay of antibiotics. Internationally designated as \u003cstrong data-start=\"702\" data-end=\"716\"\u003e“Medium B”\u003c\/strong\u003e, it is specified in all major pharmacopoeial and regulatory standards, including \u003cstrong data-start=\"798\" data-end=\"810\"\u003eUSP \u0026lt;81\u0026gt;\u003c\/strong\u003e, \u003cstrong data-start=\"812\" data-end=\"844\"\u003eEuropean Pharmacopoeia 2.7.2\u003c\/strong\u003e, \u003cstrong data-start=\"846\" data-end=\"871\"\u003eBritish Pharmacopoeia\u003c\/strong\u003e, \u003cstrong data-start=\"873\" data-end=\"907\"\u003eJapanese Pharmacopoeia (JP 17)\u003c\/strong\u003e, \u003cstrong data-start=\"909\" data-end=\"933\"\u003eFDA (21 CFR 436.102)\u003c\/strong\u003e, \u003cstrong data-start=\"935\" data-end=\"942\"\u003eWHO\u003c\/strong\u003e, and \u003cstrong data-start=\"948\" data-end=\"963\"\u003eISO 20776-2\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp data-start=\"966\" data-end=\"1257\"\u003eWhen used as a base layer and overlaid with a seeded assay layer (typically Antibiotic Medium No. 1 or No. 11), this medium produces \u003cstrong data-start=\"1099\" data-end=\"1157\"\u003elarge, sharp, and highly reproducible inhibition zones\u003c\/strong\u003e, particularly for \u003cstrong data-start=\"1176\" data-end=\"1206\"\u003eslow-diffusing antibiotics\u003c\/strong\u003e or assays requiring a \u003cstrong data-start=\"1229\" data-end=\"1256\"\u003ethicker agar foundation\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp data-start=\"1259\" data-end=\"1458\"\u003eAusamics Antibiotic Medium No. 2 is manufactured to deliver consistent diffusion performance, reliable zone definition, and full compendial compliance for pharmaceutical quality-control laboratories.\u003c\/p\u003e\n\u003ch4 data-start=\"1460\" data-end=\"1490\"\u003eKey Features \u0026amp; Benefits\u003c\/h4\u003e\n\u003cp data-start=\"1491\" data-end=\"1570\"\u003eInternationally harmonised formulation (USP \/ EP \/ BP \/ JP \/ WHO \/ FDA \/ ISO)\u003c\/p\u003e\n\u003cp data-start=\"1572\" data-end=\"1638\"\u003eDesigned specifically as the base layer in cylinder-plate assays\u003c\/p\u003e\n\u003cp data-start=\"1640\" data-end=\"1715\"\u003eHigher peptone and beef extract content for improved antibiotic diffusion\u003c\/p\u003e\n\u003cp data-start=\"1717\" data-end=\"1791\"\u003eSlightly higher pH (6.9–7.0) than Medium No. 1 for enhanced zone clarity\u003c\/p\u003e\n\u003cp data-start=\"1793\" data-end=\"1859\"\u003eProduces larger, clearer, and more reproducible inhibition zones\u003c\/p\u003e\n\u003cp data-start=\"1861\" data-end=\"1919\"\u003eGold-standard base medium for slow-diffusing antibiotics\u003c\/p\u003e\n\u003ch4 data-start=\"1921\" data-end=\"1948\"\u003eTypical Applications\u003c\/h4\u003e\n\u003cp data-start=\"1949\" data-end=\"2017\"\u003eBase layer in cylinder-plate (cup-plate) antibiotic potency assays\u003c\/p\u003e\n\u003cp data-start=\"2019\" data-end=\"2083\"\u003eAssay of aminoglycosides (gentamicin, streptomycin, kanamycin)\u003c\/p\u003e\n\u003cp data-start=\"2085\" data-end=\"2156\"\u003eAssay of macrolides, lincosamides, tetracyclines, and chloramphenicol\u003c\/p\u003e\n\u003cp data-start=\"2158\" data-end=\"2198\"\u003ePharmaceutical quality-control testing\u003c\/p\u003e\n\u003cp data-start=\"2200\" data-end=\"2278\"\u003eReference medium for antibiotic content, potency, and bioequivalence studies\u003c\/p\u003e\n\u003ch4 data-start=\"2280\" data-end=\"2310\"\u003ePrinciple of the Medium\u003c\/h4\u003e\n\u003cp data-start=\"2311\" data-end=\"2655\"\u003eAntibiotic Medium No. 2 provides a \u003cstrong data-start=\"2346\" data-end=\"2379\"\u003enutrient-rich, firm agar base\u003c\/strong\u003e that supports uniform diffusion of antibiotics from cylinders or cups into the agar matrix. Its optimized nutrient balance and agar concentration ensure stable growth conditions in the overlaid seeded medium, enabling accurate zone measurement and high assay reproducibility.\u003c\/p\u003e\n\u003ch4 data-start=\"2657\" data-end=\"2695\"\u003eTypical Composition (per litre)\u003c\/h4\u003e\n\u003cp data-start=\"2696\" data-end=\"2718\"\u003eBeef Extract – 1.5 g\u003c\/p\u003e\n\u003cp data-start=\"2720\" data-end=\"2743\"\u003eYeast Extract – 3.0 g\u003c\/p\u003e\n\u003cp data-start=\"2745\" data-end=\"2779\"\u003ePeptone (meat or casein) – 6.0 g\u003c\/p\u003e\n\u003cp data-start=\"2781\" data-end=\"2800\"\u003eD-Glucose – 1.0 g\u003c\/p\u003e\n\u003cp data-start=\"2802\" data-end=\"2817\"\u003eAgar – 17.0 g\u003c\/p\u003e\n\u003cp data-start=\"2819\" data-end=\"2875\"\u003eFinal pH: \u003cstrong data-start=\"2829\" data-end=\"2873\"\u003e6.9 ± 0.1 at 25 °C (after sterilization)\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch4 data-start=\"2877\" data-end=\"2895\"\u003ePreparation\u003c\/h4\u003e\n\u003cp data-start=\"2896\" data-end=\"2955\"\u003eSuspend 28.5 g of dehydrated medium in 1 L purified water\u003c\/p\u003e\n\u003cp data-start=\"2957\" data-end=\"3034\"\u003eHeat with frequent agitation and boil for 1–2 minutes until fully dissolved\u003c\/p\u003e\n\u003cp data-start=\"3036\" data-end=\"3087\"\u003eSterilize by autoclaving at 121 °C for 15 minutes\u003c\/p\u003e\n\u003cp data-start=\"3089\" data-end=\"3107\"\u003eCool to 60–65 °C\u003c\/p\u003e\n\u003cp data-start=\"3109\" data-end=\"3200\"\u003ePour 20–25 mL into sterile 90–100 mm Petri dishes to form a uniform base layer (≈ 5–6 mm)\u003c\/p\u003e\n\u003cp data-start=\"3202\" data-end=\"3240\"\u003eAllow to solidify on a level surface\u003c\/p\u003e\n\u003ch4 data-start=\"3242\" data-end=\"3263\"\u003eStandard Usage\u003c\/h4\u003e\n\u003cp data-start=\"3264\" data-end=\"3455\"\u003eAfter solidification of the base layer, overlay with \u003cstrong data-start=\"3317\" data-end=\"3371\"\u003e4–6 mL of seeded Antibiotic Medium No. 1 or No. 11\u003c\/strong\u003e containing the appropriate test organism, following the relevant compendial method.\u003c\/p\u003e\n\u003ch4 data-start=\"3457\" data-end=\"3483\"\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cp data-start=\"3484\" data-end=\"3543\"\u003eDehydrated medium: Store below 30 °C in a cool, dry place\u003c\/p\u003e\n\u003cp data-start=\"3545\" data-end=\"3628\"\u003ePrepared base-layer plates: Store at 2–8 °C, protected from light and desiccation\u003c\/p\u003e\n\u003cp data-start=\"3630\" data-end=\"3679\"\u003eShelf life (prepared plates): Use within 7 days\u003c\/p\u003e\n\u003ch4 data-start=\"3681\" data-end=\"3700\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp data-start=\"3701\" data-end=\"3842\"\u003eFor pharmaceutical quality-control and compendial antibiotic assay applications only.\u003cbr data-start=\"3786\" data-end=\"3789\"\u003eNot for clinical diagnostic, food, or veterinary use.\u003c\/p\u003e\n\u003ch4 data-start=\"3844\" data-end=\"3871\"\u003eQuality \u0026amp; Compliance\u003c\/h4\u003e\n\u003cp data-start=\"3872\" data-end=\"3927\"\u003eMeets USP, EP, BP, JP, FDA, WHO, and ISO requirements\u003c\/p\u003e\n\u003cp data-start=\"3929\" data-end=\"3994\"\u003eSuitable for regulated pharmaceutical microbiology laboratories\u003c\/p\u003e\n\u003cp data-start=\"3996\" data-end=\"4061\"\u003ePerformance comparable to leading international reference media\u003c\/p\u003e\n\u003ch4 data-start=\"4063\" data-end=\"4097\"\u003eCustoms \u0026amp; Trade Information\u003c\/h4\u003e\n\u003cp data-start=\"4098\" data-end=\"4205\"\u003eHS \/ AHECC Code: \u003cstrong data-start=\"4115\" data-end=\"4129\"\u003e3821.00.00\u003c\/strong\u003e\u003cbr data-start=\"4129\" data-end=\"4132\"\u003ePrepared culture media for development or maintenance of microorganisms\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"100G","offer_id":48052564066559,"sku":"AS-1109-DH-LS-100G","price":45.0,"currency_code":"AUD","in_stock":true},{"title":"500G","offer_id":48052564099327,"sku":"AS-1109-DH-LS-500G","price":125.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_6335d2f3-dddc-410c-8f7d-952b5fb23526.jpg?v=1771063036","url":"https:\/\/ausamics.com.au\/products\/antibiotic-medium-no-2-penassay-base-agar","provider":"AuSaMicS","version":"1.0","type":"link"}