{"product_id":"antibiotic-medium-no-5-streptomycin-assay","title":"Antibiotic Medium No. 5","description":"\u003ch3 data-start=\"559\" data-end=\"617\"\u003eAntibiotic Medium No. 5 (Streptomycin Base-Layer Agar)\u003c\/h3\u003e\n\u003cp data-start=\"619\" data-end=\"704\"\u003eOfficial Base Agar for Cylinder-Plate Antibiotic Assays\u003cbr data-start=\"674\" data-end=\"677\"\u003eCatalog Number: \u003cstrong data-start=\"693\" data-end=\"704\"\u003eAS-1118\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"706\" data-end=\"709\"\u003e\n\u003ch4 data-start=\"711\" data-end=\"724\"\u003eOverview\u003c\/h4\u003e\n\u003cp data-start=\"726\" data-end=\"1032\"\u003eAntibiotic Medium No. 5 is the official standardized \u003cstrong data-start=\"779\" data-end=\"798\"\u003ebase-layer agar\u003c\/strong\u003e used for the microbiological cylinder-plate assay of \u003cstrong data-start=\"852\" data-end=\"892\"\u003estreptomycin and dihydrostreptomycin\u003c\/strong\u003e. It is designated as \u003cstrong data-start=\"914\" data-end=\"935\"\u003e“Medium D (base)”\u003c\/strong\u003e in USP \u0026lt;81\u0026gt;, European Pharmacopoeia 2.7.2, Japanese Pharmacopoeia (JP 17), and WHO guidelines.\u003c\/p\u003e\n\u003cp data-start=\"1034\" data-end=\"1313\"\u003eThe slightly alkaline pH (\u003cstrong data-start=\"1060\" data-end=\"1071\"\u003e7.8–8.0\u003c\/strong\u003e) and enriched yeast–beef composition are specifically optimized to maximize streptomycin diffusion and to produce exceptionally large, clear, and reproducible inhibition zones when overlaid with seeded \u003cstrong data-start=\"1274\" data-end=\"1301\"\u003eAntibiotic Medium No. 4\u003c\/strong\u003e (or No. 8).\u003c\/p\u003e\n\u003cp data-start=\"1315\" data-end=\"1416\"\u003eThis medium is used exclusively as the base layer in official compendial streptomycin potency assays.\u003c\/p\u003e\n\u003chr data-start=\"1418\" data-end=\"1421\"\u003e\n\u003ch4 data-start=\"1423\" data-end=\"1440\"\u003eApplications\u003c\/h4\u003e\n\u003cul data-start=\"1442\" data-end=\"1664\"\u003e\n\u003cli data-start=\"1442\" data-end=\"1523\"\u003e\n\u003cp data-start=\"1444\" data-end=\"1523\"\u003eCompendial base layer for streptomycin and dihydrostreptomycin potency assays\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1524\" data-end=\"1610\"\u003e\n\u003cp data-start=\"1526\" data-end=\"1610\"\u003eCylinder-plate diffusion assays of streptomycin-containing pharmaceutical products\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1611\" data-end=\"1664\"\u003e\n\u003cp data-start=\"1613\" data-end=\"1664\"\u003eRegulatory pharmaceutical quality-control testing\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"1666\" data-end=\"1669\"\u003e\n\u003ch4 data-start=\"1671\" data-end=\"1699\"\u003eKey Features \u0026amp; Benefits\u003c\/h4\u003e\n\u003cul data-start=\"1701\" data-end=\"2000\"\u003e\n\u003cli data-start=\"1701\" data-end=\"1779\"\u003e\n\u003cp data-start=\"1703\" data-end=\"1779\"\u003eSlightly alkaline pH (\u003cstrong data-start=\"1725\" data-end=\"1732\"\u003e7.9\u003c\/strong\u003e) critical for maximum streptomycin diffusion\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1780\" data-end=\"1863\"\u003e\n\u003cp data-start=\"1782\" data-end=\"1863\"\u003eHigher yeast extract level than standard base media for larger inhibition zones\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1864\" data-end=\"1928\"\u003e\n\u003cp data-start=\"1866\" data-end=\"1928\"\u003eInternationally harmonized formulation (USP \/ EP \/ JP \/ WHO)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1929\" data-end=\"2000\"\u003e\n\u003cp data-start=\"1931\" data-end=\"2000\"\u003eOfficial reference base agar for all compendial streptomycin assays\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"2002\" data-end=\"2005\"\u003e\n\u003ch4 data-start=\"2007\" data-end=\"2035\"\u003eComposition (per litre)\u003c\/h4\u003e\n\u003cul data-start=\"2037\" data-end=\"2164\"\u003e\n\u003cli data-start=\"2037\" data-end=\"2061\"\u003e\n\u003cp data-start=\"2039\" data-end=\"2061\"\u003eBeef Extract – 1.5 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2062\" data-end=\"2087\"\u003e\n\u003cp data-start=\"2064\" data-end=\"2087\"\u003eYeast Extract – 6.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2088\" data-end=\"2124\"\u003e\n\u003cp data-start=\"2090\" data-end=\"2124\"\u003ePeptone (meat or casein) – 6.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2125\" data-end=\"2146\"\u003e\n\u003cp data-start=\"2127\" data-end=\"2146\"\u003eD-Glucose – 1.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2147\" data-end=\"2164\"\u003e\n\u003cp data-start=\"2149\" data-end=\"2164\"\u003eAgar – 17.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2166\" data-end=\"2241\"\u003eFinal pH: \u003cstrong data-start=\"2176\" data-end=\"2198\"\u003e7.9 ± 0.1 at 25 °C\u003c\/strong\u003e (after sterilisation; strictly controlled)\u003c\/p\u003e\n\u003chr data-start=\"2243\" data-end=\"2246\"\u003e\n\u003ch4 data-start=\"2248\" data-end=\"2264\"\u003ePreparation\u003c\/h4\u003e\n\u003cul data-start=\"2266\" data-end=\"2834\"\u003e\n\u003cli data-start=\"2266\" data-end=\"2335\"\u003e\n\u003cp data-start=\"2268\" data-end=\"2335\"\u003eSuspend \u003cstrong data-start=\"2276\" data-end=\"2286\"\u003e31.5 g\u003c\/strong\u003e of dehydrated medium in \u003cstrong data-start=\"2311\" data-end=\"2318\"\u003e1 L\u003c\/strong\u003e purified water\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2336\" data-end=\"2424\"\u003e\n\u003cp data-start=\"2338\" data-end=\"2424\"\u003eHeat with frequent agitation and boil for \u003cstrong data-start=\"2380\" data-end=\"2395\"\u003e1–2 minutes\u003c\/strong\u003e until completely dissolved\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2425\" data-end=\"2482\"\u003e\n\u003cp data-start=\"2427\" data-end=\"2482\"\u003eSterilise by autoclaving at \u003cstrong data-start=\"2455\" data-end=\"2480\"\u003e121 °C for 15 minutes\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2483\" data-end=\"2507\"\u003e\n\u003cp data-start=\"2485\" data-end=\"2507\"\u003eCool to \u003cstrong data-start=\"2493\" data-end=\"2505\"\u003e60–65 °C\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2508\" data-end=\"2633\"\u003e\n\u003cp data-start=\"2510\" data-end=\"2633\"\u003ePour \u003cstrong data-start=\"2515\" data-end=\"2527\"\u003e20–25 mL\u003c\/strong\u003e into sterile \u003cstrong data-start=\"2541\" data-end=\"2554\"\u003e90–100 mm\u003c\/strong\u003e Petri dishes to form a uniform base layer\u003cbr data-start=\"2596\" data-end=\"2599\"\u003e\u003cem data-start=\"2601\" data-end=\"2631\"\u003e(approximately 5–6 mm thick)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2634\" data-end=\"2674\"\u003e\n\u003cp data-start=\"2636\" data-end=\"2674\"\u003eAllow to solidify on a level surface\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2675\" data-end=\"2834\"\u003e\n\u003cp data-start=\"2677\" data-end=\"2834\"\u003eOverlay with \u003cstrong data-start=\"2690\" data-end=\"2700\"\u003e4–6 mL\u003c\/strong\u003e of seeded \u003cstrong data-start=\"2711\" data-end=\"2749\"\u003eAntibiotic Medium No. 4 (or No. 8)\u003c\/strong\u003e containing\u003cbr data-start=\"2760\" data-end=\"2763\"\u003e\u003cem data-start=\"2765\" data-end=\"2788\"\u003eKlebsiella pneumoniae\u003c\/em\u003e ATCC 10031 or \u003cem data-start=\"2803\" data-end=\"2822\"\u003eBacillus subtilis\u003c\/em\u003e ATCC 6633\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"2836\" data-end=\"2839\"\u003e\n\u003ch4 data-start=\"2841\" data-end=\"2865\"\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cul data-start=\"2867\" data-end=\"3006\"\u003e\n\u003cli data-start=\"2867\" data-end=\"2932\"\u003e\n\u003cp data-start=\"2869\" data-end=\"2932\"\u003e\u003cstrong data-start=\"2869\" data-end=\"2891\"\u003eDehydrated medium:\u003c\/strong\u003e Store below 30 °C in a cool, dry place\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2933\" data-end=\"3006\"\u003e\n\u003cp data-start=\"2935\" data-end=\"3006\"\u003e\u003cstrong data-start=\"2935\" data-end=\"2966\"\u003ePrepared base-layer plates:\u003c\/strong\u003e Store at 2–8 °C and use within 7 days\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3008\" data-end=\"3011\"\u003e\n\u003ch4 data-start=\"3013\" data-end=\"3062\"\u003eMandatory Combination for Streptomycin Assay\u003c\/h4\u003e\n\u003cul data-start=\"3064\" data-end=\"3178\"\u003e\n\u003cli data-start=\"3064\" data-end=\"3107\"\u003e\n\u003cp data-start=\"3066\" data-end=\"3107\"\u003e\u003cstrong data-start=\"3066\" data-end=\"3081\"\u003eBase layer:\u003c\/strong\u003e Antibiotic Medium No. 5\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3108\" data-end=\"3178\"\u003e\n\u003cp data-start=\"3110\" data-end=\"3178\"\u003e\u003cstrong data-start=\"3110\" data-end=\"3125\"\u003eSeed layer:\u003c\/strong\u003e Antibiotic Medium No. 4 (or No. 8) + test organism\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3180\" data-end=\"3183\"\u003e\n\u003ch4 data-start=\"3185\" data-end=\"3202\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp data-start=\"3204\" data-end=\"3356\"\u003eFor pharmaceutical quality-control and official compendial streptomycin assay applications only.\u003cbr data-start=\"3300\" data-end=\"3303\"\u003eNot for clinical diagnostic, food, or veterinary use.\u003c\/p\u003e\n\u003chr data-start=\"3358\" data-end=\"3361\"\u003e\n\u003ch4 data-start=\"3363\" data-end=\"3388\"\u003eQuality \u0026amp; Compliance\u003c\/h4\u003e\n\u003cul data-start=\"3390\" data-end=\"3648\"\u003e\n\u003cli data-start=\"3390\" data-end=\"3459\"\u003e\n\u003cp data-start=\"3392\" data-end=\"3459\"\u003eOfficial base-layer medium for streptomycin cylinder-plate assays\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3460\" data-end=\"3527\"\u003e\n\u003cp data-start=\"3462\" data-end=\"3527\"\u003eComplies with USP \u0026lt;81\u0026gt;, EP 2.7.2, JP 17, and WHO requirements\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3528\" data-end=\"3603\"\u003e\n\u003cp data-start=\"3530\" data-end=\"3603\"\u003eManufactured under controlled conditions for batch-to-batch consistency\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3604\" data-end=\"3648\"\u003e\n\u003cp data-start=\"3606\" data-end=\"3648\"\u003eCOA, SDS, and TDS available upon request\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3650\" data-end=\"3653\"\u003e\n\u003ch4 data-start=\"3655\" data-end=\"3687\"\u003eCustoms \u0026amp; Trade Information\u003c\/h4\u003e\n\u003cp data-start=\"3689\" data-end=\"3794\"\u003eHS \/ AHECC Code: \u003cstrong data-start=\"3706\" data-end=\"3720\"\u003e3821.00.00\u003c\/strong\u003e\u003cbr data-start=\"3720\" data-end=\"3723\"\u003ePrepared culture media for development or maintenance of microorganisms\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"100G","offer_id":48052757004543,"sku":"AS-1118-DH-LS-100G","price":55.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_e1b94a2e-9a95-45b9-ba0b-ba6679b9f170.jpg?v=1771063030","url":"https:\/\/ausamics.com.au\/products\/antibiotic-medium-no-5-streptomycin-assay","provider":"AuSaMicS","version":"1.0","type":"link"}