{"product_id":"arginine-decarboxylase-broth","title":"Arginine Decarboxylase Broth","description":"\u003ch3 data-start=\"595\" data-end=\"627\"\u003eArginine Decarboxylase Broth\u003c\/h3\u003e\n\u003cp data-start=\"629\" data-end=\"715\"\u003eDifferential Medium for Arginine Decarboxylation Testing\u003cbr data-start=\"685\" data-end=\"688\"\u003eCatalog Number: \u003cstrong data-start=\"704\" data-end=\"715\"\u003eAS-1123\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"717\" data-end=\"720\"\u003e\n\u003ch4 data-start=\"722\" data-end=\"735\"\u003eOverview\u003c\/h4\u003e\n\u003cp data-start=\"737\" data-end=\"1131\"\u003eArginine Decarboxylase Broth is a \u003cstrong data-start=\"771\" data-end=\"801\"\u003edifferential liquid medium\u003c\/strong\u003e used to detect the ability of Enterobacteriaceae and other bacteria to \u003cstrong data-start=\"873\" data-end=\"943\"\u003edecarboxylate the amino acid L-arginine under anaerobic conditions\u003c\/strong\u003e. Decarboxylation of arginine produces alkaline amines (agmatine and putrescine), resulting in a characteristic \u003cstrong data-start=\"1055\" data-end=\"1091\"\u003epH shift from acidic to alkaline\u003c\/strong\u003e following initial glucose fermentation.\u003c\/p\u003e\n\u003cp data-start=\"1133\" data-end=\"1473\"\u003eThe medium contains \u003cstrong data-start=\"1153\" data-end=\"1174\"\u003ebromcresol purple\u003c\/strong\u003e as a pH indicator. After an initial acid phase (yellow), organisms possessing arginine decarboxylase cause re-alkalinisation of the medium, producing a \u003cstrong data-start=\"1327\" data-end=\"1354\"\u003eviolet or purple colour\u003c\/strong\u003e. The test is performed under anaerobic conditions created by overlaying the inoculated broth with sterile mineral oil.\u003c\/p\u003e\n\u003cp data-start=\"1475\" data-end=\"1702\"\u003eArginine Decarboxylase Broth is part of classical biochemical identification schemes related to the IMViC system and is widely used in laboratory workflows for the differentiation and characterisation of Gram-negative bacteria.\u003c\/p\u003e\n\u003chr data-start=\"1704\" data-end=\"1707\"\u003e\n\u003ch4 data-start=\"1709\" data-end=\"1726\"\u003eApplications\u003c\/h4\u003e\n\u003cul data-start=\"1728\" data-end=\"2059\"\u003e\n\u003cli data-start=\"1728\" data-end=\"1782\"\u003e\n\u003cp data-start=\"1730\" data-end=\"1782\"\u003eBiochemical characterisation of Enterobacteriaceae\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1783\" data-end=\"1883\"\u003e\n\u003cp data-start=\"1785\" data-end=\"1883\"\u003eDifferentiation of \u003cem data-start=\"1804\" data-end=\"1816\"\u003eKlebsiella\u003c\/em\u003e and \u003cem data-start=\"1821\" data-end=\"1835\"\u003eEnterobacter\u003c\/em\u003e (positive) from \u003cem data-start=\"1852\" data-end=\"1870\"\u003eEscherichia coli\u003c\/em\u003e (negative)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1884\" data-end=\"1983\"\u003e\n\u003cp data-start=\"1886\" data-end=\"1983\"\u003eConfirmation of reactions in \u003cem data-start=\"1915\" data-end=\"1927\"\u003eSalmonella\u003c\/em\u003e, \u003cem data-start=\"1929\" data-end=\"1942\"\u003eCitrobacter\u003c\/em\u003e, \u003cem data-start=\"1944\" data-end=\"1953\"\u003eProteus\u003c\/em\u003e, \u003cem data-start=\"1955\" data-end=\"1965\"\u003eSerratia\u003c\/em\u003e, and \u003cem data-start=\"1971\" data-end=\"1981\"\u003eYersinia\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1984\" data-end=\"2059\"\u003e\n\u003cp data-start=\"1986\" data-end=\"2059\"\u003eUse in standard biochemical test batteries and identification workflows\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"2061\" data-end=\"2064\"\u003e\n\u003ch4 data-start=\"2066\" data-end=\"2094\"\u003eKey Features \u0026amp; Benefits\u003c\/h4\u003e\n\u003cul data-start=\"2096\" data-end=\"2344\"\u003e\n\u003cli data-start=\"2096\" data-end=\"2153\"\u003e\n\u003cp data-start=\"2098\" data-end=\"2153\"\u003eDetects arginine decarboxylase activity via pH change\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2154\" data-end=\"2222\"\u003e\n\u003cp data-start=\"2156\" data-end=\"2222\"\u003eBromcresol purple indicator provides clear visual interpretation\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2223\" data-end=\"2288\"\u003e\n\u003cp data-start=\"2225\" data-end=\"2288\"\u003eRequires anaerobic conditions for correct reaction expression\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2289\" data-end=\"2344\"\u003e\n\u003cp data-start=\"2291\" data-end=\"2344\"\u003eClassical, well-established biochemical test medium\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"2346\" data-end=\"2349\"\u003e\n\u003ch4 data-start=\"2351\" data-end=\"2379\"\u003ePrinciple of the Medium\u003c\/h4\u003e\n\u003cp data-start=\"2381\" data-end=\"2734\"\u003eGlucose fermentation initially lowers the pH of the medium, turning the indicator yellow. Under anaerobic conditions, bacteria capable of arginine decarboxylation convert arginine into alkaline amines, causing the pH to rise and the indicator to return to a \u003cstrong data-start=\"2639\" data-end=\"2666\"\u003eviolet or purple colour\u003c\/strong\u003e. Organisms lacking arginine decarboxylase remain acidic and yellow.\u003c\/p\u003e\n\u003chr data-start=\"2736\" data-end=\"2739\"\u003e\n\u003ch4 data-start=\"2741\" data-end=\"2769\"\u003eComposition (per litre)\u003c\/h4\u003e\n\u003cul data-start=\"2771\" data-end=\"2962\"\u003e\n\u003cli data-start=\"2771\" data-end=\"2807\"\u003e\n\u003cp data-start=\"2773\" data-end=\"2807\"\u003eL-Arginine hydrochloride – 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2808\" data-end=\"2845\"\u003e\n\u003cp data-start=\"2810\" data-end=\"2845\"\u003ePeptone (or meat extract) – 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2846\" data-end=\"2865\"\u003e\n\u003cp data-start=\"2848\" data-end=\"2865\"\u003eGlucose – 0.5 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2866\" data-end=\"2896\"\u003e\n\u003cp data-start=\"2868\" data-end=\"2896\"\u003eBromcresol Purple – 0.02 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2897\" data-end=\"2962\"\u003e\n\u003cp data-start=\"2899\" data-end=\"2962\"\u003ePyridoxal phosphate – 0.005 g \u003cem data-start=\"2929\" data-end=\"2960\"\u003e(optional; enhances reaction)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2964\" data-end=\"2996\"\u003eFinal pH: \u003cstrong data-start=\"2974\" data-end=\"2996\"\u003e6.0 ± 0.2 at 25 °C\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"2998\" data-end=\"3001\"\u003e\n\u003ch4 data-start=\"3003\" data-end=\"3019\"\u003ePreparation\u003c\/h4\u003e\n\u003cul data-start=\"3021\" data-end=\"3364\"\u003e\n\u003cli data-start=\"3021\" data-end=\"3110\"\u003e\n\u003cp data-start=\"3023\" data-end=\"3110\"\u003eDissolve peptone, L-arginine hydrochloride, and glucose in \u003cstrong data-start=\"3082\" data-end=\"3092\"\u003e990 mL\u003c\/strong\u003e distilled water\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3111\" data-end=\"3135\"\u003e\n\u003cp data-start=\"3113\" data-end=\"3135\"\u003eAdjust pH to \u003cstrong data-start=\"3126\" data-end=\"3133\"\u003e6.0\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3136\" data-end=\"3193\"\u003e\n\u003cp data-start=\"3138\" data-end=\"3193\"\u003eAdd \u003cstrong data-start=\"3142\" data-end=\"3151\"\u003e10 mL\u003c\/strong\u003e of \u003cstrong data-start=\"3155\" data-end=\"3191\"\u003e0.2 % bromcresol purple solution\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3194\" data-end=\"3237\"\u003e\n\u003cp data-start=\"3196\" data-end=\"3237\"\u003eMake up to \u003cstrong data-start=\"3207\" data-end=\"3214\"\u003e1 L\u003c\/strong\u003e with distilled water\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3238\" data-end=\"3306\"\u003e\n\u003cp data-start=\"3240\" data-end=\"3306\"\u003eDispense \u003cstrong data-start=\"3249\" data-end=\"3259\"\u003e3–5 mL\u003c\/strong\u003e into small tubes (approximately 10 × 100 mm)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3307\" data-end=\"3364\"\u003e\n\u003cp data-start=\"3309\" data-end=\"3364\"\u003eSterilise by autoclaving at \u003cstrong data-start=\"3337\" data-end=\"3362\"\u003e121 °C for 15 minutes\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3366\" data-end=\"3369\"\u003e\n\u003ch4 data-start=\"3371\" data-end=\"3404\"\u003eInoculation \u0026amp; Interpretation\u003c\/h4\u003e\n\u003cul data-start=\"3406\" data-end=\"3598\"\u003e\n\u003cli data-start=\"3406\" data-end=\"3458\"\u003e\n\u003cp data-start=\"3408\" data-end=\"3458\"\u003eInoculate heavily with a fresh bacterial culture\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3459\" data-end=\"3550\"\u003e\n\u003cp data-start=\"3461\" data-end=\"3550\"\u003eOverlay with \u003cstrong data-start=\"3474\" data-end=\"3485\"\u003e5–10 mm\u003c\/strong\u003e sterile mineral oil or paraffin to ensure anaerobic conditions\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3551\" data-end=\"3598\"\u003e\n\u003cp data-start=\"3553\" data-end=\"3598\"\u003eIncubate at \u003cstrong data-start=\"3565\" data-end=\"3577\"\u003e35–37 °C\u003c\/strong\u003e for up to \u003cstrong data-start=\"3588\" data-end=\"3598\"\u003e4 days\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"3600\" data-end=\"3619\"\u003e\u003cstrong data-start=\"3600\" data-end=\"3619\"\u003eInterpretation:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-start=\"3620\" data-end=\"3844\"\u003e\n\u003cli data-start=\"3620\" data-end=\"3685\"\u003e\n\u003cp data-start=\"3622\" data-end=\"3685\"\u003e\u003cstrong data-start=\"3622\" data-end=\"3644\"\u003eYellow throughout:\u003c\/strong\u003e Negative (no arginine decarboxylation)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3686\" data-end=\"3762\"\u003e\n\u003cp data-start=\"3688\" data-end=\"3762\"\u003e\u003cstrong data-start=\"3688\" data-end=\"3718\"\u003eViolet\/alkaline under oil:\u003c\/strong\u003e Positive (arginine decarboxylase present)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3763\" data-end=\"3844\"\u003e\n\u003cp data-start=\"3765\" data-end=\"3844\"\u003e\u003cstrong data-start=\"3765\" data-end=\"3816\"\u003eYellow initially, turning purple after 24–48 h:\u003c\/strong\u003e Typical positive reaction\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3846\" data-end=\"3849\"\u003e\n\u003ch4 data-start=\"3851\" data-end=\"3875\"\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cul data-start=\"3877\" data-end=\"3986\"\u003e\n\u003cli data-start=\"3877\" data-end=\"3942\"\u003e\n\u003cp data-start=\"3879\" data-end=\"3942\"\u003e\u003cstrong data-start=\"3879\" data-end=\"3898\"\u003ePrepared tubes:\u003c\/strong\u003e Store at \u003cstrong data-start=\"3908\" data-end=\"3918\"\u003e2–8 °C\u003c\/strong\u003e, protected from light\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3943\" data-end=\"3986\"\u003e\n\u003cp data-start=\"3945\" data-end=\"3986\"\u003e\u003cstrong data-start=\"3945\" data-end=\"3960\"\u003eShelf life:\u003c\/strong\u003e Use within \u003cstrong data-start=\"3972\" data-end=\"3984\"\u003e3 months\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3988\" data-end=\"3991\"\u003e\n\u003ch4 data-start=\"3993\" data-end=\"4010\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp data-start=\"4012\" data-end=\"4126\"\u003eFor laboratory identification and biochemical characterisation use only.\u003cbr data-start=\"4084\" data-end=\"4087\"\u003eNot for direct clinical diagnostic use.\u003c\/p\u003e\n\u003chr data-start=\"4128\" data-end=\"4131\"\u003e\n\u003ch4 data-start=\"4133\" data-end=\"4158\"\u003eQuality \u0026amp; Compliance\u003c\/h4\u003e\n\u003cul data-start=\"4160\" data-end=\"4362\"\u003e\n\u003cli data-start=\"4160\" data-end=\"4228\"\u003e\n\u003cp data-start=\"4162\" data-end=\"4228\"\u003eClassical biochemical test medium for amino acid decarboxylation\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4229\" data-end=\"4289\"\u003e\n\u003cp data-start=\"4231\" data-end=\"4289\"\u003eManufactured under controlled conditions for consistency\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4290\" data-end=\"4362\"\u003e\n\u003cp data-start=\"4292\" data-end=\"4362\"\u003eSuitable for research, teaching, and method-development laboratories\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"4364\" data-end=\"4367\"\u003e\n\u003ch4 data-start=\"4369\" data-end=\"4401\"\u003eCustoms \u0026amp; Trade Information\u003c\/h4\u003e\n\u003cp data-start=\"4403\" data-end=\"4508\"\u003eHS \/ AHECC Code: \u003cstrong data-start=\"4420\" data-end=\"4434\"\u003e3821.00.00\u003c\/strong\u003e\u003cbr data-start=\"4434\" data-end=\"4437\"\u003ePrepared culture media for development or maintenance of microorganisms\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"100G","offer_id":48052788494591,"sku":"AS-1123-DH-LS-100G","price":45.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_2712312b-40ac-464c-a792-e3a521c97022.jpg?v=1771063028","url":"https:\/\/ausamics.com.au\/products\/arginine-decarboxylase-broth","provider":"AuSaMicS","version":"1.0","type":"link"}