{"product_id":"arginine-dihydrolase-broth","title":"Arginine Dihydrolase Broth","description":"\u003ch3 data-start=\"534\" data-end=\"564\"\u003eArginine Dihydrolase Broth\u003c\/h3\u003e\n\u003cp data-start=\"566\" data-end=\"654\"\u003eDifferential Medium for Arginine Dihydrolase (ADH) Testing\u003cbr data-start=\"624\" data-end=\"627\"\u003eCatalog Number: \u003cstrong data-start=\"643\" data-end=\"654\"\u003eAS-1124\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"656\" data-end=\"659\"\u003e\n\u003ch4 data-start=\"661\" data-end=\"674\"\u003eOverview\u003c\/h4\u003e\n\u003cp data-start=\"676\" data-end=\"1114\"\u003eArginine Dihydrolase Broth is a \u003cstrong data-start=\"708\" data-end=\"738\"\u003edifferential liquid medium\u003c\/strong\u003e used to detect the ability of \u003cstrong data-start=\"769\" data-end=\"808\"\u003enon-fermentative Gram-negative rods\u003c\/strong\u003e to produce ammonia from \u003cstrong data-start=\"833\" data-end=\"890\"\u003eL-arginine via the arginine dihydrolase (ADH) pathway\u003c\/strong\u003e under anaerobic conditions. Typical target organisms include \u003cem data-start=\"952\" data-end=\"976\"\u003ePseudomonas aeruginosa\u003c\/em\u003e, \u003cem data-start=\"978\" data-end=\"998\"\u003ePseudomonas putida\u003c\/em\u003e, \u003cem data-start=\"1000\" data-end=\"1025\"\u003ePseudomonas fluorescens\u003c\/em\u003e, \u003cem data-start=\"1027\" data-end=\"1049\"\u003eBurkholderia cepacia\u003c\/em\u003e, \u003cem data-start=\"1051\" data-end=\"1066\"\u003eAchromobacter\u003c\/em\u003e spp., \u003cem data-start=\"1073\" data-end=\"1086\"\u003eAlcaligenes\u003c\/em\u003e spp., and \u003cem data-start=\"1097\" data-end=\"1109\"\u003eBordetella\u003c\/em\u003e spp.\u003c\/p\u003e\n\u003cp data-start=\"1116\" data-end=\"1391\"\u003eA positive reaction results in a strong \u003cstrong data-start=\"1156\" data-end=\"1174\"\u003ealkaline shift\u003c\/strong\u003e, producing a colour change from \u003cstrong data-start=\"1207\" data-end=\"1245\"\u003ecolourless to bright purple-violet\u003c\/strong\u003e due to ammonia production. The test is performed under anaerobic conditions created by overlaying the inoculated medium with sterile mineral oil.\u003c\/p\u003e\n\u003cp data-start=\"1393\" data-end=\"1561\"\u003eArginine Dihydrolase Broth is a core component of classical identification schemes for \u003cstrong data-start=\"1480\" data-end=\"1536\"\u003enon-enteric, oxidase-positive Gram-negative bacteria\u003c\/strong\u003e in laboratory workflows.\u003c\/p\u003e\n\u003chr data-start=\"1563\" data-end=\"1566\"\u003e\n\u003ch4 data-start=\"1568\" data-end=\"1585\"\u003eApplications\u003c\/h4\u003e\n\u003cul data-start=\"1587\" data-end=\"1933\"\u003e\n\u003cli data-start=\"1587\" data-end=\"1658\"\u003e\n\u003cp data-start=\"1589\" data-end=\"1658\"\u003eIdentification of \u003cem data-start=\"1607\" data-end=\"1631\"\u003ePseudomonas aeruginosa\u003c\/em\u003e and related pseudomonads\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1659\" data-end=\"1752\"\u003e\n\u003cp data-start=\"1661\" data-end=\"1752\"\u003eDifferentiation of \u003cem data-start=\"1680\" data-end=\"1694\"\u003eBurkholderia\u003c\/em\u003e, \u003cem data-start=\"1696\" data-end=\"1714\"\u003eStenotrophomonas\u003c\/em\u003e, \u003cem data-start=\"1716\" data-end=\"1731\"\u003eAchromobacter\u003c\/em\u003e, and \u003cem data-start=\"1737\" data-end=\"1750\"\u003eAlcaligenes\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1753\" data-end=\"1855\"\u003e\n\u003cp data-start=\"1755\" data-end=\"1855\"\u003ePart of classical identification schemes (King’s scheme, API 20NE, VITEK GNI, and related systems)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1856\" data-end=\"1933\"\u003e\n\u003cp data-start=\"1858\" data-end=\"1933\"\u003eConfirmation of oxidase-positive, non-fermentative Gram-negative isolates\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"1935\" data-end=\"1938\"\u003e\n\u003ch4 data-start=\"1940\" data-end=\"1968\"\u003eKey Features \u0026amp; Benefits\u003c\/h4\u003e\n\u003cul data-start=\"1970\" data-end=\"2254\"\u003e\n\u003cli data-start=\"1970\" data-end=\"2040\"\u003e\n\u003cp data-start=\"1972\" data-end=\"2040\"\u003eDetects arginine dihydrolase (ADH) activity via ammonia production\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2041\" data-end=\"2122\"\u003e\n\u003cp data-start=\"2043\" data-end=\"2122\"\u003eRapid positive reactions in \u003cem data-start=\"2071\" data-end=\"2095\"\u003ePseudomonas aeruginosa\u003c\/em\u003e (often within 4–8 hours)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2123\" data-end=\"2181\"\u003e\n\u003cp data-start=\"2125\" data-end=\"2181\"\u003eClear colour endpoint using bromothymol blue indicator\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2182\" data-end=\"2254\"\u003e\n\u003cp data-start=\"2184\" data-end=\"2254\"\u003eRequires anaerobic conditions for correct expression of the reaction\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"2256\" data-end=\"2259\"\u003e\n\u003ch4 data-start=\"2261\" data-end=\"2289\"\u003ePrinciple of the Medium\u003c\/h4\u003e\n\u003cp data-start=\"2291\" data-end=\"2655\"\u003eUnder anaerobic conditions, organisms possessing the arginine dihydrolase pathway convert arginine to ammonia, resulting in alkalinisation of the medium. The pH indicator \u003cstrong data-start=\"2462\" data-end=\"2482\"\u003ebromothymol blue\u003c\/strong\u003e responds to this increase in pH, producing a \u003cstrong data-start=\"2528\" data-end=\"2559\"\u003ebright purple-violet colour\u003c\/strong\u003e. Organisms lacking ADH activity do not alkalinise the medium, which remains colourless or pale.\u003c\/p\u003e\n\u003chr data-start=\"2657\" data-end=\"2660\"\u003e\n\u003ch4 data-start=\"2662\" data-end=\"2690\"\u003eComposition (per litre)\u003c\/h4\u003e\n\u003cul data-start=\"2692\" data-end=\"2856\"\u003e\n\u003cli data-start=\"2692\" data-end=\"2728\"\u003e\n\u003cp data-start=\"2694\" data-end=\"2728\"\u003eL-Arginine hydrochloride – 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2729\" data-end=\"2748\"\u003e\n\u003cp data-start=\"2731\" data-end=\"2748\"\u003ePeptone – 1.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2749\" data-end=\"2783\"\u003e\n\u003cp data-start=\"2751\" data-end=\"2783\"\u003eSodium chloride (NaCl) – 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2784\" data-end=\"2826\"\u003e\n\u003cp data-start=\"2786\" data-end=\"2826\"\u003eDipotassium phosphate (K₂HPO₄) – 0.3 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2827\" data-end=\"2856\"\u003e\n\u003cp data-start=\"2829\" data-end=\"2856\"\u003eBromothymol Blue – 0.03 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2858\" data-end=\"2890\"\u003eFinal pH: \u003cstrong data-start=\"2868\" data-end=\"2890\"\u003e7.0 ± 0.2 at 25 °C\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"2892\" data-end=\"2895\"\u003e\n\u003ch4 data-start=\"2897\" data-end=\"2913\"\u003ePreparation\u003c\/h4\u003e\n\u003cul data-start=\"2915\" data-end=\"3286\"\u003e\n\u003cli data-start=\"2915\" data-end=\"3034\"\u003e\n\u003cp data-start=\"2917\" data-end=\"3034\"\u003eDissolve peptone, sodium chloride, dipotassium phosphate, and L-arginine hydrochloride in \u003cstrong data-start=\"3007\" data-end=\"3017\"\u003e970 mL\u003c\/strong\u003e purified water\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3035\" data-end=\"3059\"\u003e\n\u003cp data-start=\"3037\" data-end=\"3059\"\u003eAdjust pH to \u003cstrong data-start=\"3050\" data-end=\"3057\"\u003e7.0\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3060\" data-end=\"3116\"\u003e\n\u003cp data-start=\"3062\" data-end=\"3116\"\u003eAdd \u003cstrong data-start=\"3066\" data-end=\"3075\"\u003e30 mL\u003c\/strong\u003e of \u003cstrong data-start=\"3079\" data-end=\"3114\"\u003e0.1 % bromothymol blue solution\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3117\" data-end=\"3159\"\u003e\n\u003cp data-start=\"3119\" data-end=\"3159\"\u003eMake up to \u003cstrong data-start=\"3130\" data-end=\"3137\"\u003e1 L\u003c\/strong\u003e with purified water\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3160\" data-end=\"3228\"\u003e\n\u003cp data-start=\"3162\" data-end=\"3228\"\u003eDispense \u003cstrong data-start=\"3171\" data-end=\"3181\"\u003e3–4 mL\u003c\/strong\u003e into small tubes (approximately 10 × 100 mm)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3229\" data-end=\"3286\"\u003e\n\u003cp data-start=\"3231\" data-end=\"3286\"\u003eSterilise by autoclaving at \u003cstrong data-start=\"3259\" data-end=\"3284\"\u003e121 °C for 15 minutes\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3288\" data-end=\"3291\"\u003e\n\u003ch4 data-start=\"3293\" data-end=\"3326\"\u003eInoculation \u0026amp; Interpretation\u003c\/h4\u003e\n\u003cul data-start=\"3328\" data-end=\"3637\"\u003e\n\u003cli data-start=\"3328\" data-end=\"3380\"\u003e\n\u003cp data-start=\"3330\" data-end=\"3380\"\u003eInoculate heavily with a fresh bacterial culture\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3381\" data-end=\"3475\"\u003e\n\u003cp data-start=\"3383\" data-end=\"3475\"\u003eOverlay immediately with \u003cstrong data-start=\"3408\" data-end=\"3418\"\u003e5–8 mm\u003c\/strong\u003e sterile mineral oil or paraffin to ensure anaerobiosis\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3476\" data-end=\"3504\"\u003e\n\u003cp data-start=\"3478\" data-end=\"3504\"\u003eIncubate at \u003cstrong data-start=\"3490\" data-end=\"3502\"\u003e35–37 °C\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3505\" data-end=\"3637\"\u003e\n\u003cp data-start=\"3507\" data-end=\"3637\"\u003eExamine after \u003cstrong data-start=\"3521\" data-end=\"3532\"\u003e4 hours\u003c\/strong\u003e, \u003cstrong data-start=\"3534\" data-end=\"3545\"\u003e8 hours\u003c\/strong\u003e, and \u003cstrong data-start=\"3551\" data-end=\"3563\"\u003e24 hours\u003c\/strong\u003e\u003cbr data-start=\"3563\" data-end=\"3566\"\u003e\u003cem data-start=\"3568\" data-end=\"3637\"\u003e(incubation may be extended up to 7 days for slow-reacting strains)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"3639\" data-end=\"3658\"\u003e\u003cstrong data-start=\"3639\" data-end=\"3658\"\u003eInterpretation:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-start=\"3659\" data-end=\"3786\"\u003e\n\u003cli data-start=\"3659\" data-end=\"3739\"\u003e\n\u003cp data-start=\"3661\" data-end=\"3739\"\u003e\u003cstrong data-start=\"3661\" data-end=\"3697\"\u003eBright purple-violet throughout:\u003c\/strong\u003e Positive (arginine dihydrolase present)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3740\" data-end=\"3786\"\u003e\n\u003cp data-start=\"3742\" data-end=\"3786\"\u003e\u003cstrong data-start=\"3742\" data-end=\"3775\"\u003eGreenish or no colour change:\u003c\/strong\u003e Negative\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3788\" data-end=\"3791\"\u003e\n\u003ch4 data-start=\"3793\" data-end=\"3817\"\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cul data-start=\"3819\" data-end=\"3928\"\u003e\n\u003cli data-start=\"3819\" data-end=\"3884\"\u003e\n\u003cp data-start=\"3821\" data-end=\"3884\"\u003e\u003cstrong data-start=\"3821\" data-end=\"3840\"\u003ePrepared tubes:\u003c\/strong\u003e Store at \u003cstrong data-start=\"3850\" data-end=\"3860\"\u003e2–8 °C\u003c\/strong\u003e, protected from light\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3885\" data-end=\"3928\"\u003e\n\u003cp data-start=\"3887\" data-end=\"3928\"\u003e\u003cstrong data-start=\"3887\" data-end=\"3902\"\u003eShelf life:\u003c\/strong\u003e Use within \u003cstrong data-start=\"3914\" data-end=\"3926\"\u003e3 months\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3930\" data-end=\"3933\"\u003e\n\u003ch4 data-start=\"3935\" data-end=\"3952\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp data-start=\"3954\" data-end=\"4068\"\u003eFor laboratory identification and biochemical characterisation use only.\u003cbr data-start=\"4026\" data-end=\"4029\"\u003eNot for direct clinical diagnostic use.\u003c\/p\u003e\n\u003chr data-start=\"4070\" data-end=\"4073\"\u003e\n\u003ch4 data-start=\"4075\" data-end=\"4100\"\u003eQuality \u0026amp; Compliance\u003c\/h4\u003e\n\u003cul data-start=\"4102\" data-end=\"4317\"\u003e\n\u003cli data-start=\"4102\" data-end=\"4183\"\u003e\n\u003cp data-start=\"4104\" data-end=\"4183\"\u003eClassical biochemical test medium for non-fermentative Gram-negative bacteria\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4184\" data-end=\"4244\"\u003e\n\u003cp data-start=\"4186\" data-end=\"4244\"\u003eManufactured under controlled conditions for consistency\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4245\" data-end=\"4317\"\u003e\n\u003cp data-start=\"4247\" data-end=\"4317\"\u003eSuitable for research, teaching, and method-development laboratories\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"4319\" data-end=\"4322\"\u003e\n\u003ch4 data-start=\"4324\" data-end=\"4356\"\u003eCustoms \u0026amp; Trade Information\u003c\/h4\u003e\n\u003cp data-start=\"4358\" data-end=\"4463\"\u003eHS \/ AHECC Code: \u003cstrong data-start=\"4375\" data-end=\"4389\"\u003e3821.00.00\u003c\/strong\u003e\u003cbr data-start=\"4389\" data-end=\"4392\"\u003ePrepared culture media for development or maintenance of microorganisms\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"100G","offer_id":48052790657279,"sku":"AS-1124-DH-LS-100G","price":45.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_afc5e0df-6200-4ac1-acdf-638ddb03ce07.jpg?v=1771063028","url":"https:\/\/ausamics.com.au\/products\/arginine-dihydrolase-broth","provider":"AuSaMicS","version":"1.0","type":"link"}