{"product_id":"asparagine-broth","title":"Asparagine Broth","description":"\u003ch3 data-start=\"500\" data-end=\"520\"\u003eAsparagine Broth\u003c\/h3\u003e\n\u003cp data-start=\"522\" data-end=\"621\"\u003eChemically Defined Medium for Mycobacterial Nitrate Reduction Testing\u003cbr data-start=\"591\" data-end=\"594\"\u003eCatalog Number: \u003cstrong data-start=\"610\" data-end=\"621\"\u003eAS-1125\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"623\" data-end=\"626\"\u003e\n\u003ch4 data-start=\"628\" data-end=\"641\"\u003eOverview\u003c\/h4\u003e\n\u003cp data-start=\"643\" data-end=\"917\"\u003eAsparagine Broth is a \u003cstrong data-start=\"665\" data-end=\"710\"\u003eminimal, chemically defined liquid medium\u003c\/strong\u003e used for the detection of \u003cstrong data-start=\"737\" data-end=\"814\"\u003enitrate reduction and denitrification activity in \u003cem data-start=\"789\" data-end=\"804\"\u003eMycobacterium\u003c\/em\u003e species\u003c\/strong\u003e, including members of the \u003cem data-start=\"841\" data-end=\"869\"\u003eMycobacterium tuberculosis\u003c\/em\u003e complex and non-tuberculous mycobacteria (NTM).\u003c\/p\u003e\n\u003cp data-start=\"919\" data-end=\"1284\"\u003eThe medium is a key component of the \u003cstrong data-start=\"956\" data-end=\"982\"\u003eclassical Wayne method\u003c\/strong\u003e and remains in use in reference and research laboratories for phenotypic differentiation of \u003cem data-start=\"1075\" data-end=\"1092\"\u003eM. tuberculosis\u003c\/em\u003e complex (typically nitrate-negative) from most NTM (typically nitrate-positive). \u003cstrong data-start=\"1174\" data-end=\"1236\"\u003eL-asparagine serves as the sole carbon and nitrogen source\u003c\/strong\u003e, ensuring high specificity and reproducibility.\u003c\/p\u003e\n\u003cp data-start=\"1286\" data-end=\"1469\"\u003eNitrate reduction results in the formation of nitrite, which is detected by the development of a \u003cstrong data-start=\"1383\" data-end=\"1397\"\u003ered colour\u003c\/strong\u003e following the addition of sulfanilic acid and α-naphthylamine reagents.\u003c\/p\u003e\n\u003chr data-start=\"1471\" data-end=\"1474\"\u003e\n\u003ch4 data-start=\"1476\" data-end=\"1493\"\u003eApplications\u003c\/h4\u003e\n\u003cul data-start=\"1495\" data-end=\"1867\"\u003e\n\u003cli data-start=\"1495\" data-end=\"1618\"\u003e\n\u003cp data-start=\"1497\" data-end=\"1618\"\u003eDifferentiation of \u003cem data-start=\"1516\" data-end=\"1544\"\u003eMycobacterium tuberculosis\u003c\/em\u003e complex (negative) from non-tuberculous mycobacteria (usually positive)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1619\" data-end=\"1678\"\u003e\n\u003cp data-start=\"1621\" data-end=\"1678\"\u003eDetection of nitrate reductase activity in mycobacteria\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1679\" data-end=\"1767\"\u003e\n\u003cp data-start=\"1681\" data-end=\"1767\"\u003eComponent of standard phenotypic identification panels (with niacin, catalase, etc.)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1768\" data-end=\"1867\"\u003e\n\u003cp data-start=\"1770\" data-end=\"1867\"\u003eReference method described in WHO, CLSI M24-A2, and national tuberculosis laboratory guidelines\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"1869\" data-end=\"1872\"\u003e\n\u003ch4 data-start=\"1874\" data-end=\"1902\"\u003eKey Features \u0026amp; Benefits\u003c\/h4\u003e\n\u003cul data-start=\"1904\" data-end=\"2167\"\u003e\n\u003cli data-start=\"1904\" data-end=\"1964\"\u003e\n\u003cp data-start=\"1906\" data-end=\"1964\"\u003eChemically defined formulation with no complex nutrients\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1965\" data-end=\"2044\"\u003e\n\u003cp data-start=\"1967\" data-end=\"2044\"\u003eClear, colourless medium allows easy visual detection of positive reactions\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2045\" data-end=\"2110\"\u003e\n\u003cp data-start=\"2047\" data-end=\"2110\"\u003eHigh specificity for mycobacterial nitrate reductase activity\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2111\" data-end=\"2167\"\u003e\n\u003cp data-start=\"2113\" data-end=\"2167\"\u003eLong-established and well-validated reference method\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"2169\" data-end=\"2172\"\u003e\n\u003ch4 data-start=\"2174\" data-end=\"2202\"\u003ePrinciple of the Medium\u003c\/h4\u003e\n\u003cp data-start=\"2204\" data-end=\"2551\"\u003eMycobacteria capable of nitrate reduction convert nitrate to nitrite during incubation. Following incubation, nitrite is detected by the addition of sulfanilic acid and α-naphthylamine, producing a \u003cstrong data-start=\"2402\" data-end=\"2416\"\u003ered colour\u003c\/strong\u003e. Organisms lacking nitrate reductase activity do not produce nitrite, and no colour develops unless nitrate is reduced beyond nitrite.\u003c\/p\u003e\n\u003chr data-start=\"2553\" data-end=\"2556\"\u003e\n\u003ch4 data-start=\"2558\" data-end=\"2586\"\u003eComposition (per litre)\u003c\/h4\u003e\n\u003cul data-start=\"2588\" data-end=\"2869\"\u003e\n\u003cli data-start=\"2588\" data-end=\"2624\"\u003e\n\u003cp data-start=\"2590\" data-end=\"2624\"\u003eL-Asparagine (anhydrous) – 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2625\" data-end=\"2676\"\u003e\n\u003cp data-start=\"2627\" data-end=\"2676\"\u003ePotassium dihydrogen phosphate (KH₂PO₄) – 1.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2677\" data-end=\"2726\"\u003e\n\u003cp data-start=\"2679\" data-end=\"2726\"\u003eDisodium hydrogen phosphate (Na₂HPO₄) – 2.5 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2727\" data-end=\"2783\"\u003e\n\u003cp data-start=\"2729\" data-end=\"2783\"\u003eMagnesium sulfate heptahydrate (MgSO₄·7H₂O) – 0.01 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2784\" data-end=\"2810\"\u003e\n\u003cp data-start=\"2786\" data-end=\"2810\"\u003eSodium citrate – 0.1 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2811\" data-end=\"2832\"\u003e\n\u003cp data-start=\"2813\" data-end=\"2832\"\u003eGlycerol – 5.0 mL\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2833\" data-end=\"2869\"\u003e\n\u003cp data-start=\"2835\" data-end=\"2869\"\u003ePotassium nitrate (KNO₃) – 1.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2871\" data-end=\"2903\"\u003eFinal pH: \u003cstrong data-start=\"2881\" data-end=\"2903\"\u003e6.6 ± 0.2 at 25 °C\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"2905\" data-end=\"2908\"\u003e\n\u003ch4 data-start=\"2910\" data-end=\"2926\"\u003ePreparation\u003c\/h4\u003e\n\u003cul data-start=\"2928\" data-end=\"3342\"\u003e\n\u003cli data-start=\"2928\" data-end=\"2985\"\u003e\n\u003cp data-start=\"2930\" data-end=\"2985\"\u003eDissolve all components in \u003cstrong data-start=\"2957\" data-end=\"2967\"\u003e995 mL\u003c\/strong\u003e distilled water\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2986\" data-end=\"3047\"\u003e\n\u003cp data-start=\"2988\" data-end=\"3047\"\u003eAdjust pH to \u003cstrong data-start=\"3001\" data-end=\"3008\"\u003e6.6\u003c\/strong\u003e with 1 N NaOH or 1 N HCl if required\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3048\" data-end=\"3091\"\u003e\n\u003cp data-start=\"3050\" data-end=\"3091\"\u003eMake up to \u003cstrong data-start=\"3061\" data-end=\"3068\"\u003e1 L\u003c\/strong\u003e with distilled water\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3092\" data-end=\"3161\"\u003e\n\u003cp data-start=\"3094\" data-end=\"3161\"\u003eDispense \u003cstrong data-start=\"3103\" data-end=\"3113\"\u003e3–4 mL\u003c\/strong\u003e into small screw-cap tubes (e.g. 13 × 100 mm)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3162\" data-end=\"3254\"\u003e\n\u003cp data-start=\"3164\" data-end=\"3254\"\u003eSterilise by autoclaving at \u003cstrong data-start=\"3192\" data-end=\"3222\"\u003e121 °C for 10 minutes only\u003c\/strong\u003e\u003cbr data-start=\"3222\" data-end=\"3225\"\u003e\u003cem data-start=\"3227\" data-end=\"3252\"\u003e(do not over-sterilise)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3255\" data-end=\"3283\"\u003e\n\u003cp data-start=\"3257\" data-end=\"3283\"\u003eCool to room temperature\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3284\" data-end=\"3342\"\u003e\n\u003cp data-start=\"3286\" data-end=\"3342\"\u003ePrepared medium should remain \u003cstrong data-start=\"3316\" data-end=\"3340\"\u003eclear and colourless\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3344\" data-end=\"3347\"\u003e\n\u003ch4 data-start=\"3349\" data-end=\"3378\"\u003eInoculation \u0026amp; Incubation\u003c\/h4\u003e\n\u003cul data-start=\"3380\" data-end=\"3590\"\u003e\n\u003cli data-start=\"3380\" data-end=\"3482\"\u003e\n\u003cp data-start=\"3382\" data-end=\"3482\"\u003eInoculate heavily with fresh growth from Löwenstein–Jensen or Middlebrook 7H11 agar (1–2 loopfuls)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3483\" data-end=\"3512\"\u003e\n\u003cp data-start=\"3485\" data-end=\"3512\"\u003eIncubate at \u003cstrong data-start=\"3497\" data-end=\"3510\"\u003e36 ± 1 °C\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3513\" data-end=\"3549\"\u003e\n\u003cp data-start=\"3515\" data-end=\"3549\"\u003eExamine at \u003cstrong data-start=\"3526\" data-end=\"3547\"\u003e3, 7, and 14 days\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3550\" data-end=\"3590\"\u003e\n\u003cp data-start=\"3552\" data-end=\"3590\"\u003eInclude an uninoculated control tube\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3592\" data-end=\"3595\"\u003e\n\u003ch4 data-start=\"3597\" data-end=\"3626\"\u003eReading \u0026amp; Interpretation\u003c\/h4\u003e\n\u003cp data-start=\"3628\" data-end=\"3645\"\u003eAfter incubation:\u003c\/p\u003e\n\u003col data-start=\"3647\" data-end=\"3806\"\u003e\n\u003cli data-start=\"3647\" data-end=\"3726\"\u003e\n\u003cp data-start=\"3650\" data-end=\"3726\"\u003eAdd \u003cstrong data-start=\"3654\" data-end=\"3674\"\u003e0.5 mL Reagent A\u003c\/strong\u003e\u003cbr data-start=\"3674\" data-end=\"3677\"\u003e\u003cem data-start=\"3680\" data-end=\"3724\"\u003e(0.2 % sulfanilic acid in 5 N acetic acid)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3727\" data-end=\"3806\"\u003e\n\u003cp data-start=\"3730\" data-end=\"3806\"\u003eAdd \u003cstrong data-start=\"3734\" data-end=\"3754\"\u003e0.5 mL Reagent B\u003c\/strong\u003e\u003cbr data-start=\"3754\" data-end=\"3757\"\u003e\u003cem data-start=\"3760\" data-end=\"3804\"\u003e(0.1 % α-naphthylamine in 5 N acetic acid)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-start=\"3808\" data-end=\"3820\"\u003e\u003cstrong data-start=\"3808\" data-end=\"3820\"\u003eResults:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-start=\"3821\" data-end=\"3915\"\u003e\n\u003cli data-start=\"3821\" data-end=\"3887\"\u003e\n\u003cp data-start=\"3823\" data-end=\"3887\"\u003e\u003cstrong data-start=\"3823\" data-end=\"3858\"\u003eRed colour within 5–10 minutes:\u003c\/strong\u003e Positive (nitrite present)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3888\" data-end=\"3915\"\u003e\n\u003cp data-start=\"3890\" data-end=\"3915\"\u003e\u003cstrong data-start=\"3890\" data-end=\"3904\"\u003eNo colour:\u003c\/strong\u003e Negative\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"3917\" data-end=\"3976\"\u003eIf no colour develops, add a small amount of \u003cstrong data-start=\"3962\" data-end=\"3975\"\u003ezinc dust\u003c\/strong\u003e:\u003c\/p\u003e\n\u003cul data-start=\"3977\" data-end=\"4099\"\u003e\n\u003cli data-start=\"3977\" data-end=\"4021\"\u003e\n\u003cp data-start=\"3979\" data-end=\"4021\"\u003eRed colour after zinc: \u003cstrong data-start=\"4002\" data-end=\"4019\"\u003etrue negative\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4022\" data-end=\"4099\"\u003e\n\u003cp data-start=\"4024\" data-end=\"4099\"\u003eNo colour after zinc: \u003cstrong data-start=\"4046\" data-end=\"4064\"\u003efalse negative\u003c\/strong\u003e (nitrate reduced beyond nitrite)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"4101\" data-end=\"4104\"\u003e\n\u003ch4 data-start=\"4106\" data-end=\"4130\"\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cul data-start=\"4132\" data-end=\"4241\"\u003e\n\u003cli data-start=\"4132\" data-end=\"4197\"\u003e\n\u003cp data-start=\"4134\" data-end=\"4197\"\u003e\u003cstrong data-start=\"4134\" data-end=\"4153\"\u003ePrepared tubes:\u003c\/strong\u003e Store at \u003cstrong data-start=\"4163\" data-end=\"4173\"\u003e2–8 °C\u003c\/strong\u003e, protected from light\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4198\" data-end=\"4241\"\u003e\n\u003cp data-start=\"4200\" data-end=\"4241\"\u003e\u003cstrong data-start=\"4200\" data-end=\"4215\"\u003eShelf life:\u003c\/strong\u003e Use within \u003cstrong data-start=\"4227\" data-end=\"4239\"\u003e6 months\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"4243\" data-end=\"4246\"\u003e\n\u003ch4 data-start=\"4248\" data-end=\"4265\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp data-start=\"4267\" data-end=\"4361\"\u003eFor laboratory identification and research use only.\u003cbr data-start=\"4319\" data-end=\"4322\"\u003eNot for direct clinical diagnostic use.\u003c\/p\u003e\n\u003chr data-start=\"4363\" data-end=\"4366\"\u003e\n\u003ch4 data-start=\"4368\" data-end=\"4393\"\u003eQuality \u0026amp; Compliance\u003c\/h4\u003e\n\u003cul data-start=\"4395\" data-end=\"4610\"\u003e\n\u003cli data-start=\"4395\" data-end=\"4469\"\u003e\n\u003cp data-start=\"4397\" data-end=\"4469\"\u003eClassical reference medium for mycobacterial nitrate reduction testing\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4470\" data-end=\"4536\"\u003e\n\u003cp data-start=\"4472\" data-end=\"4536\"\u003eManufactured under controlled conditions for batch consistency\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4537\" data-end=\"4610\"\u003e\n\u003cp data-start=\"4539\" data-end=\"4610\"\u003eSuitable for research, reference, and method-development laboratories\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"4612\" data-end=\"4615\"\u003e\n\u003ch4 data-start=\"4617\" data-end=\"4649\"\u003eCustoms \u0026amp; Trade Information\u003c\/h4\u003e\n\u003cp data-start=\"4651\" data-end=\"4756\"\u003eHS \/ AHECC Code: \u003cstrong data-start=\"4668\" data-end=\"4682\"\u003e3821.00.00\u003c\/strong\u003e\u003cbr data-start=\"4682\" data-end=\"4685\"\u003ePrepared culture media for development or maintenance of microorganisms\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"100G","offer_id":48052792623359,"sku":"AS-1125-DH-LS-100G","price":55.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_81f0e6a1-a9f5-40f1-a2d9-a670757a9080.jpg?v=1771063027","url":"https:\/\/ausamics.com.au\/products\/asparagine-broth","provider":"AuSaMicS","version":"1.0","type":"link"}