{"product_id":"asparagine-proline-broth","title":"Asparagine Proline Broth","description":"\u003ch3 data-start=\"499\" data-end=\"527\"\u003eAsparagine Proline Broth\u003c\/h3\u003e\n\u003cp data-start=\"529\" data-end=\"615\"\u003eChemically Defined Medium for Rapid-Growing Mycobacteria\u003cbr data-start=\"585\" data-end=\"588\"\u003eCatalog Number: \u003cstrong data-start=\"604\" data-end=\"615\"\u003eAS-1126\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"617\" data-end=\"620\"\u003e\n\u003ch4 data-start=\"622\" data-end=\"635\"\u003eOverview\u003c\/h4\u003e\n\u003cp data-start=\"637\" data-end=\"925\"\u003eAsparagine Proline Broth is a \u003cstrong data-start=\"667\" data-end=\"712\"\u003echemically defined, minimal liquid medium\u003c\/strong\u003e used as a key phenotypic test for the identification of the \u003cstrong data-start=\"773\" data-end=\"817\"\u003eMycobacterium fortuitum–chelonae complex\u003c\/strong\u003e, including \u003cem data-start=\"829\" data-end=\"854\"\u003eMycobacterium fortuitum\u003c\/em\u003e, \u003cem data-start=\"856\" data-end=\"869\"\u003eM. chelonae\u003c\/em\u003e, \u003cem data-start=\"871\" data-end=\"885\"\u003eM. abscessus\u003c\/em\u003e, \u003cem data-start=\"887\" data-end=\"902\"\u003eM. peregrinum\u003c\/em\u003e, and \u003cem data-start=\"908\" data-end=\"924\"\u003eM. mucogenicum\u003c\/em\u003e.\u003c\/p\u003e\n\u003cp data-start=\"927\" data-end=\"1281\"\u003eOnly members of this rapid-growing mycobacterial group are able to utilize \u003cstrong data-start=\"1002\" data-end=\"1046\"\u003eL-asparagine as the sole nitrogen source\u003c\/strong\u003e and \u003cstrong data-start=\"1051\" data-end=\"1090\"\u003eL-proline as the sole carbon source\u003c\/strong\u003e, resulting in visible \u003cstrong data-start=\"1113\" data-end=\"1148\"\u003eturbidity or sediment formation\u003c\/strong\u003e within \u003cstrong data-start=\"1156\" data-end=\"1168\"\u003e3–7 days\u003c\/strong\u003e of incubation. Slow-growing mycobacteria and most other non-tuberculous mycobacteria do not grow in this medium.\u003c\/p\u003e\n\u003cp data-start=\"1283\" data-end=\"1455\"\u003eThis test remains recommended in reference laboratory workflows and by international guidelines when molecular methods are unavailable or as a phenotypic confirmation step.\u003c\/p\u003e\n\u003chr data-start=\"1457\" data-end=\"1460\"\u003e\n\u003ch4 data-start=\"1462\" data-end=\"1479\"\u003eApplications\u003c\/h4\u003e\n\u003cul data-start=\"1481\" data-end=\"1804\"\u003e\n\u003cli data-start=\"1481\" data-end=\"1567\"\u003e\n\u003cp data-start=\"1483\" data-end=\"1567\"\u003eRapid presumptive identification of the \u003cem data-start=\"1523\" data-end=\"1557\"\u003eMycobacterium fortuitum–chelonae\u003c\/em\u003e complex\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1568\" data-end=\"1673\"\u003e\n\u003cp data-start=\"1570\" data-end=\"1673\"\u003eDifferentiation of rapid-growing mycobacteria from \u003cem data-start=\"1621\" data-end=\"1649\"\u003eMycobacterium tuberculosis\u003c\/em\u003e complex and other NTM\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1674\" data-end=\"1804\"\u003e\n\u003cp data-start=\"1676\" data-end=\"1804\"\u003eComponent of classical phenotypic test batteries for rapid growers\u003cbr data-start=\"1742\" data-end=\"1745\"\u003e\u003cem data-start=\"1747\" data-end=\"1802\"\u003e(e.g. 5 % NaCl tolerance, arylsulfatase, iron uptake)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"1806\" data-end=\"1809\"\u003e\n\u003ch4 data-start=\"1811\" data-end=\"1839\"\u003eKey Features \u0026amp; Benefits\u003c\/h4\u003e\n\u003cul data-start=\"1841\" data-end=\"2132\"\u003e\n\u003cli data-start=\"1841\" data-end=\"1921\"\u003e\n\u003cp data-start=\"1843\" data-end=\"1921\"\u003eHighly specific growth response limited to the \u003cem data-start=\"1890\" data-end=\"1913\"\u003eM. fortuitum–chelonae\u003c\/em\u003e group\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1922\" data-end=\"1993\"\u003e\n\u003cp data-start=\"1924\" data-end=\"1993\"\u003eChemically defined formulation with minimal background interference\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1994\" data-end=\"2061\"\u003e\n\u003cp data-start=\"1996\" data-end=\"2061\"\u003eClear baseline medium allows easy visual detection of turbidity\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2062\" data-end=\"2132\"\u003e\n\u003cp data-start=\"2064\" data-end=\"2132\"\u003ePositive reactions typically observed within \u003cstrong data-start=\"2109\" data-end=\"2121\"\u003e3–5 days\u003c\/strong\u003e at 30 °C\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"2134\" data-end=\"2137\"\u003e\n\u003ch4 data-start=\"2139\" data-end=\"2167\"\u003ePrinciple of the Medium\u003c\/h4\u003e\n\u003cp data-start=\"2169\" data-end=\"2522\"\u003eAsparagine Proline Broth contains \u003cstrong data-start=\"2203\" data-end=\"2247\"\u003eL-asparagine as the sole nitrogen source\u003c\/strong\u003e and \u003cstrong data-start=\"2252\" data-end=\"2291\"\u003eL-proline as the sole carbon source\u003c\/strong\u003e. Rapid-growing mycobacteria of the \u003cem data-start=\"2327\" data-end=\"2350\"\u003eM. fortuitum–chelonae\u003c\/em\u003e complex are able to metabolize both substrates efficiently, resulting in visible growth. Organisms lacking this metabolic capability remain in a clear, growth-free medium.\u003c\/p\u003e\n\u003chr data-start=\"2524\" data-end=\"2527\"\u003e\n\u003ch4 data-start=\"2529\" data-end=\"2557\"\u003eComposition (per litre)\u003c\/h4\u003e\n\u003cul data-start=\"2559\" data-end=\"2981\"\u003e\n\u003cli data-start=\"2559\" data-end=\"2583\"\u003e\n\u003cp data-start=\"2561\" data-end=\"2583\"\u003eL-Asparagine – 3.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2584\" data-end=\"2605\"\u003e\n\u003cp data-start=\"2586\" data-end=\"2605\"\u003eL-Proline – 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2606\" data-end=\"2657\"\u003e\n\u003cp data-start=\"2608\" data-end=\"2657\"\u003ePotassium dihydrogen phosphate (KH₂PO₄) – 1.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2658\" data-end=\"2707\"\u003e\n\u003cp data-start=\"2660\" data-end=\"2707\"\u003eDisodium hydrogen phosphate (Na₂HPO₄) – 1.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2708\" data-end=\"2763\"\u003e\n\u003cp data-start=\"2710\" data-end=\"2763\"\u003eMagnesium sulfate heptahydrate (MgSO₄·7H₂O) – 0.5 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2764\" data-end=\"2817\"\u003e\n\u003cp data-start=\"2766\" data-end=\"2817\"\u003eCalcium chloride dihydrate (CaCl₂·2H₂O) – 0.001 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2818\" data-end=\"2870\"\u003e\n\u003cp data-start=\"2820\" data-end=\"2870\"\u003eZinc sulfate heptahydrate (ZnSO₄·7H₂O) – 0.001 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2871\" data-end=\"2925\"\u003e\n\u003cp data-start=\"2873\" data-end=\"2925\"\u003eCopper sulfate pentahydrate (CuSO₄·5H₂O) – 0.001 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2926\" data-end=\"2981\"\u003e\n\u003cp data-start=\"2928\" data-end=\"2981\"\u003eFerrous sulfate heptahydrate (FeSO₄·7H₂O) – 0.001 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2983\" data-end=\"3015\"\u003eFinal pH: \u003cstrong data-start=\"2993\" data-end=\"3015\"\u003e6.8 ± 0.2 at 25 °C\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"3017\" data-end=\"3020\"\u003e\n\u003ch4 data-start=\"3022\" data-end=\"3038\"\u003ePreparation\u003c\/h4\u003e\n\u003cul data-start=\"3040\" data-end=\"3405\"\u003e\n\u003cli data-start=\"3040\" data-end=\"3102\"\u003e\n\u003cp data-start=\"3042\" data-end=\"3102\"\u003eDissolve all inorganic salts in \u003cstrong data-start=\"3074\" data-end=\"3084\"\u003e900 mL\u003c\/strong\u003e distilled water\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3103\" data-end=\"3162\"\u003e\n\u003cp data-start=\"3105\" data-end=\"3162\"\u003eAdd L-asparagine and L-proline; warm gently if required\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3163\" data-end=\"3187\"\u003e\n\u003cp data-start=\"3165\" data-end=\"3187\"\u003eAdjust pH to \u003cstrong data-start=\"3178\" data-end=\"3185\"\u003e6.8\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3188\" data-end=\"3231\"\u003e\n\u003cp data-start=\"3190\" data-end=\"3231\"\u003eMake up to \u003cstrong data-start=\"3201\" data-end=\"3208\"\u003e1 L\u003c\/strong\u003e with distilled water\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3232\" data-end=\"3340\"\u003e\n\u003cp data-start=\"3234\" data-end=\"3340\"\u003e\u003cstrong data-start=\"3234\" data-end=\"3281\"\u003eFilter-sterilise through a 0.22 µm membrane\u003c\/strong\u003e\u003cbr data-start=\"3281\" data-end=\"3284\"\u003e\u003cem data-start=\"3286\" data-end=\"3338\"\u003e(do not autoclave; amino acids are heat-sensitive)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3341\" data-end=\"3405\"\u003e\n\u003cp data-start=\"3343\" data-end=\"3405\"\u003eDispense \u003cstrong data-start=\"3352\" data-end=\"3362\"\u003e3–4 mL\u003c\/strong\u003e aseptically into sterile screw-cap tubes\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3407\" data-end=\"3410\"\u003e\n\u003ch4 data-start=\"3412\" data-end=\"3441\"\u003eInoculation \u0026amp; Incubation\u003c\/h4\u003e\n\u003cul data-start=\"3443\" data-end=\"3720\"\u003e\n\u003cli data-start=\"3443\" data-end=\"3542\"\u003e\n\u003cp data-start=\"3445\" data-end=\"3542\"\u003eInoculate with \u003cstrong data-start=\"3460\" data-end=\"3476\"\u003e1–2 loopfuls\u003c\/strong\u003e of fresh growth from Löwenstein–Jensen or Middlebrook 7H11 agar\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3543\" data-end=\"3583\"\u003e\n\u003cp data-start=\"3545\" data-end=\"3583\"\u003eInclude an uninoculated control tube\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3584\" data-end=\"3632\"\u003e\n\u003cp data-start=\"3586\" data-end=\"3632\"\u003eIncubate at \u003cstrong data-start=\"3598\" data-end=\"3611\"\u003e30 ± 2 °C\u003c\/strong\u003e with caps loosened\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3633\" data-end=\"3720\"\u003e\n\u003cp data-start=\"3635\" data-end=\"3720\"\u003eExamine daily for up to \u003cstrong data-start=\"3659\" data-end=\"3669\"\u003e7 days\u003c\/strong\u003e\u003cbr data-start=\"3669\" data-end=\"3672\"\u003e\u003cem data-start=\"3674\" data-end=\"3718\"\u003e(growth may be visible as early as 3 days)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3722\" data-end=\"3725\"\u003e\n\u003ch4 data-start=\"3727\" data-end=\"3746\"\u003eInterpretation\u003c\/h4\u003e\n\u003cul data-start=\"3748\" data-end=\"3913\"\u003e\n\u003cli data-start=\"3748\" data-end=\"3866\"\u003e\n\u003cp data-start=\"3750\" data-end=\"3866\"\u003e\u003cstrong data-start=\"3750\" data-end=\"3763\"\u003ePositive:\u003c\/strong\u003e Distinct turbidity or heavy sediment formation\u003cbr data-start=\"3810\" data-end=\"3813\"\u003e\u003cem data-start=\"3815\" data-end=\"3864\"\u003e(indicative of \u003cem data-start=\"3831\" data-end=\"3854\"\u003eM. fortuitum–chelonae\u003c\/em\u003e complex)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3867\" data-end=\"3913\"\u003e\n\u003cp data-start=\"3869\" data-end=\"3913\"\u003e\u003cstrong data-start=\"3869\" data-end=\"3882\"\u003eNegative:\u003c\/strong\u003e Medium remains crystal clear\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3915\" data-end=\"3918\"\u003e\n\u003ch4 data-start=\"3920\" data-end=\"3944\"\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cul data-start=\"3946\" data-end=\"4064\"\u003e\n\u003cli data-start=\"3946\" data-end=\"4020\"\u003e\n\u003cp data-start=\"3948\" data-end=\"4020\"\u003e\u003cstrong data-start=\"3948\" data-end=\"3976\"\u003eFilter-sterilised broth:\u003c\/strong\u003e Store at \u003cstrong data-start=\"3986\" data-end=\"3996\"\u003e2–8 °C\u003c\/strong\u003e, protected from light\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4021\" data-end=\"4064\"\u003e\n\u003cp data-start=\"4023\" data-end=\"4064\"\u003e\u003cstrong data-start=\"4023\" data-end=\"4038\"\u003eShelf life:\u003c\/strong\u003e Use within \u003cstrong data-start=\"4050\" data-end=\"4062\"\u003e3 months\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"4066\" data-end=\"4069\"\u003e\n\u003ch4 data-start=\"4071\" data-end=\"4088\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp data-start=\"4090\" data-end=\"4184\"\u003eFor laboratory identification and research use only.\u003cbr data-start=\"4142\" data-end=\"4145\"\u003eNot for direct clinical diagnostic use.\u003c\/p\u003e\n\u003chr data-start=\"4186\" data-end=\"4189\"\u003e\n\u003ch4 data-start=\"4191\" data-end=\"4216\"\u003eQuality \u0026amp; Compliance\u003c\/h4\u003e\n\u003cul data-start=\"4218\" data-end=\"4411\"\u003e\n\u003cli data-start=\"4218\" data-end=\"4280\"\u003e\n\u003cp data-start=\"4220\" data-end=\"4280\"\u003eReference phenotypic medium for rapid-growing mycobacteria\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4281\" data-end=\"4347\"\u003e\n\u003cp data-start=\"4283\" data-end=\"4347\"\u003eManufactured under controlled conditions for batch consistency\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4348\" data-end=\"4411\"\u003e\n\u003cp data-start=\"4350\" data-end=\"4411\"\u003eSuitable for research, teaching, and reference laboratories\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"4413\" data-end=\"4416\"\u003e\n\u003ch4 data-start=\"4418\" data-end=\"4450\"\u003eCustoms \u0026amp; Trade Information\u003c\/h4\u003e\n\u003cp data-start=\"4452\" data-end=\"4557\"\u003eHS \/ AHECC Code: \u003cstrong data-start=\"4469\" data-end=\"4483\"\u003e3821.00.00\u003c\/strong\u003e\u003cbr data-start=\"4483\" data-end=\"4486\"\u003ePrepared culture media for development or maintenance of microorganisms\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"100G","offer_id":48052799176959,"sku":"AS-1126-DH-LS-100G","price":55.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_5fda7eb6-0557-43a5-b451-ead141712387.jpg?v=1771063026","url":"https:\/\/ausamics.com.au\/products\/asparagine-proline-broth","provider":"AuSaMicS","version":"1.0","type":"link"}