{"product_id":"atcc-2039-broth-twin-pack","title":"ATCC 2039 Broth (Twin Pack) l AS-1441","description":"\u003c!--\n=============================================================================\nLLM \/ AI-AGENT OPTIMIZATION BLOCK — AuSaMicS Life Science™\n=============================================================================\nProduct:        ATCC 2039 Broth (Twin Pack) — Solution A + Solution B\nCatalogue:      AS-1441\nReference:      Official ATCC Medium 2039 specification (atcc.org)\nSolution A:     (NH4)2SO4 0.8g + MgSO4.7H2O 2.0g + K2HPO4 0.4g + Wolfe's Mineral 5mL in 800mL DI water\n                Adjust pH 2.3 with H2SO4 — filter sterilise — DO NOT AUTOCLAVE\nWolfe's Mineral:Nitrilotriacetic acid 1.5g + MgSO4 3.0g + MnSO4 0.5g + NaCl 1.0g + FeSO4 0.1g\n                + CoCl2 0.1g + CaCl2 0.1g + ZnSO4 0.1g + CuSO4 0.01g + AlK(SO4)2 0.01g\n                + H3BO3 0.01g + Na2MoO4 0.01g in 1000mL\nSolution B:     FeSO4.7H2O 20.0g in 200mL DI water — filter sterilise IMMEDIATELY\nFinal pH:       2.3 (adjusted with H2SO4 — verify before inoculation)\nIncubation:     26–30°C, aerobic, shaking 100–150 rpm, 4–14 days\nTarget:         Acidithiobacillus ferrooxidans ATCC 23270\nEquivalents:    HiMedia M1963 | ATCC Medium 2039 reference\n=============================================================================\n--\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cstyle\u003e\n.at441{font-family:Arial,Helvetica,sans-serif;color:#1f2937;max-width:900px;margin:0 auto;line-height:1.65}\n\/* HERO — medium teal\/slate — professional, not too dark *\/\n.at441-hero{background:linear-gradient(135deg,#1e3a4a 0%,#2c5564 50%,#326878 100%);border-radius:10px 10px 0 0;padding:34px 30px;color:#fff;position:relative;overflow:hidden}\n.at441-hero::after{content:'pH 2.3';font-size:90px;font-weight:700;color:rgba(255,255,255,.05);position:absolute;right:0;bottom:-10px;pointer-events:none;font-family:monospace}\n.at441-hero h1{margin:0 0 6px;font-size:25px;font-weight:800;color:#fff;position:relative}\n.at441-hero h1 span{color:#a0dce8}\n.at441-hero p{font-size:14px;color:#90c8d8;margin:0 0 16px;max-width:720px;position:relative}\n.at441-badges{display:flex;flex-wrap:wrap;gap:6px;position:relative}\n.at441-badge{background:rgba(255,255,255,.12);border:1px solid rgba(255,255,255,.25);border-radius:20px;padding:4px 12px;font-size:11px;color:#fff;font-weight:600}\n.at441-badge.teal{background:rgba(100,200,220,.2);border-color:#70c0d0;color:#c0eef8}\n.at441-badge.green{background:rgba(72,180,100,.2);border-color:#58c870;color:#b8f0c8}\n.at441-badge.amber{background:rgba(220,170,60,.2);border-color:#d0b040;color:#f8e080}\n.at441-badge.red{background:rgba(200,60,60,.2);border-color:#d06060;color:#f8c0c0}\n\/* TWIN STRIP *\/\n.at441-twin{display:grid;grid-template-columns:1fr 1fr;background:#163040}\n.at441-tw{padding:14px 20px;border-right:1px solid rgba(255,255,255,.12)}\n.at441-tw:last-child{border-right:none}\n.at441-tw-lbl{font-size:10px;font-weight:700;color:#a0dce8;letter-spacing:.1em;text-transform:uppercase;margin-bottom:4px}\n.at441-tw-name{font-size:15px;font-weight:700;color:#fff;margin-bottom:4px}\n.at441-tw-desc{font-size:12px;color:rgba(255,255,255,.6)}\n\/* SPEC STRIP *\/\n.at441-strip{display:grid;grid-template-columns:repeat(5,1fr);background:#f0f8fa;border-left:1px solid #b0d8e0;border-right:1px solid #b0d8e0}\n.at441-si{padding:10px 13px;border-right:1px solid #b0d8e0;border-bottom:3px solid #2c5564}\n.at441-si:last-child{border-right:none}\n.at441-sl{font-size:9px;font-weight:700;color:#1e3a4a;letter-spacing:.1em;text-transform:uppercase;margin-bottom:2px}\n.at441-sv{font-size:13px;font-weight:700;color:#1f2937}\n\/* TRUST BAR *\/\n.at441-trust{display:grid;grid-template-columns:repeat(4,1fr);background:#1e3a4a}\n.at441-ti{padding:9px 13px;border-right:1px solid rgba(255,255,255,.15);font-size:11px;color:#fff}\n.at441-ti:last-child{border-right:none}\n.at441-ti strong{display:block;font-size:11px;color:#a0dce8;margin-bottom:1px}\n\/* CRITICAL BANNER *\/\n.at441-crit{background:#8b0000;border-bottom:3px solid #a00000;padding:11px 20px;display:flex;align-items:center;gap:10px}\n.at441-crit strong{font-size:13px;color:#fff}\n.at441-crit span{font-size:12px;color:#ffc8c8}\n\/* TABS CSS-only *\/\n.at441-radio{display:none}\n.at441-tabrow{display:flex;flex-wrap:wrap;gap:3px;border-bottom:2px solid #b0d8e0;background:#d8eef4;padding:7px 7px 0}\n.at441-tabrow label{background:#b0d8e0;border:1px solid #88b8c8;border-bottom:none;border-radius:5px 5px 0 0;padding:8px 15px;font-size:13px;font-weight:600;color:#1e3a4a;cursor:pointer;user-select:none;transition:background .15s}\n.at441-tabrow label:hover{background:#88b8c8}\n.at441-panels .at441-panel{display:none;background:#fff;border:1px solid #b0d8e0;border-top:none;border-radius:0 0 8px 8px;padding:26px 22px}\n#t1:checked~.at441-tabwrap .at441-panels #tp1,\n#t2:checked~.at441-tabwrap .at441-panels #tp2,\n#t3:checked~.at441-tabwrap .at441-panels #tp3,\n#t4:checked~.at441-tabwrap .at441-panels #tp4{display:block}\n#t1:checked~.at441-tabwrap .at441-tabrow label[for=t1],\n#t2:checked~.at441-tabwrap .at441-tabrow label[for=t2],\n#t3:checked~.at441-tabwrap .at441-tabrow label[for=t3],\n#t4:checked~.at441-tabwrap .at441-tabrow label[for=t4]{background:#fff;color:#2c5564;border-bottom:2px solid #fff;margin-bottom:-2px;font-weight:700}\n\/* SECTION HEADERS *\/\n.at441-sh{background:#2c5564;color:#fff;padding:7px 14px;border-radius:4px;font-size:13px;font-weight:700;margin:22px 0 10px;letter-spacing:.03em}\n.at441-sh:first-child{margin-top:0}\n\/* TABLES *\/\n.at441-tbl{width:100%;border-collapse:collapse;font-size:14px;margin-bottom:14px}\n.at441-tbl th{background:#d8eef4;color:#1e3a4a;font-weight:700;padding:9px 11px;text-align:left;border-bottom:2px solid #b0d8e0}\n.at441-tbl td{padding:8px 11px;border-bottom:1px solid #f0f8fa;color:#374151;vertical-align:top}\n.at441-tbl tr:last-child td{border-bottom:none}\n.at441-tbl tr:hover td{background:#f0f8fa}\n.at441-tbl td:first-child{font-weight:600;color:#1e3a4a}\n.at441-tbl .hi{background:#f0f8fa}\n.at441-tbl .key{color:#2c5564;font-weight:600}\n.at441-tbl .crit{color:#8b0000;font-weight:700}\n.at441-formula-total td{background:#1e3a4a!important;color:#a0dce8!important;font-weight:700!important;padding:10px 11px!important;border:none!important}\n\/* INFO BOXES *\/\n.at441-info{background:#f0f8fa;border-left:4px solid #2c5564;border-radius:0 5px 5px 0;padding:12px 16px;margin:12px 0;font-size:14px;color:#1f2937}\n.at441-green{background:#f0f6f2;border-left:4px solid #2a5240;border-radius:0 5px 5px 0;padding:12px 16px;margin:12px 0;font-size:14px;color:#1f2937}\n.at441-warn{background:#faf6ee;border-left:4px solid #8a6820;border-radius:0 5px 5px 0;padding:11px 15px;font-size:13px;color:#5a4010;margin:10px 0}\n.at441-danger{background:#fef2f2;border-left:4px solid #8b0000;border-radius:0 5px 5px 0;padding:12px 16px;font-size:13px;color:#8b0000;margin:10px 0;font-weight:600}\n\/* PREP STEPS *\/\n.at441-steps{margin:12px 0}\n.at441-step{display:flex;gap:11px;margin-bottom:9px;align-items:flex-start}\n.at441-stepn{background:#2c5564;color:#fff;font-weight:700;font-size:12px;min-width:26px;height:26px;border-radius:50%;display:flex;align-items:center;justify-content:center;flex-shrink:0}\n.at441-stept{font-size:14px;color:#374151;padding-top:3px}\n\/* APPS *\/\n.at441-apps{display:grid;grid-template-columns:repeat(auto-fill,minmax(148px,1fr));gap:10px;margin:12px 0}\n.at441-app{background:#f0f8fa;border:1px solid #b0d8e0;border-top:3px solid #2c5564;border-radius:6px;padding:12px;text-align:center}\n.at441-app .ico{font-size:22px;margin-bottom:5px}\n.at441-app .ct{font-size:13px;font-weight:600;color:#1e3a4a}\n.at441-app .cs{font-size:11px;color:#6b7280;margin-top:2px}\n\/* FAQ *\/\n.at441-faq{border:1px solid #b0d8e0;border-radius:7px;overflow:hidden;margin:12px 0}\n.at441-fq{border-bottom:1px solid #b0d8e0}\n.at441-fq:last-child{border-bottom:none}\n.at441-fqq{padding:11px 14px;font-size:14px;font-weight:600;color:#1e3a4a;background:#f0f8fa}\n.at441-fqa{padding:0 14px 11px;font-size:14px;color:#374151}\n\/* FOOTER *\/\n.at441-footer{background:#f0f8fa;border-top:3px solid #1e3a4a;border-radius:0 0 8px 8px;padding:14px 20px;font-size:12px;color:#555}\n@media(max-width:600px){.at441-hero{padding:20px 14px}.at441-hero h1{font-size:20px}.at441-twin{grid-template-columns:1fr}.at441-strip{grid-template-columns:repeat(3,1fr)}.at441-trust{grid-template-columns:repeat(2,1fr)}.at441-panels .at441-panel{padding:16px 11px}.at441-tabrow label{padding:7px 9px;font-size:12px}}\n\u003c\/style\u003e\n\u003cdiv class=\"at441\"\u003e\n\u003c!-- HERO --\u003e\n\u003cdiv class=\"at441-hero\"\u003e\n\u003ch1\u003e\n\u003cspan\u003eATCC 2039 Broth\u003c\/span\u003e (Twin Pack)\u003c\/h1\u003e\n\u003cp\u003eOfficial ATCC Medium 2039 | \u003cem\u003eAcidithiobacillus ferrooxidans\u003c\/em\u003e Culture Medium | Solution A + Solution B | Cat. No. AS-1441\u003c\/p\u003e\n\u003cdiv class=\"at441-badges\"\u003e\n\u003cspan class=\"at441-badge\"\u003eCat. No. AS-1441\u003c\/span\u003e \u003cspan class=\"at441-badge\"\u003eOfficial ATCC 2039 Formula\u003c\/span\u003e \u003cspan class=\"at441-badge\"\u003epH 2.3\u003c\/span\u003e \u003cspan class=\"at441-badge red\"\u003e⚠ DO NOT AUTOCLAVE\u003c\/span\u003e \u003cspan class=\"at441-badge teal\"\u003e🏢 Bioleaching Research\u003c\/span\u003e \u003cspan class=\"at441-badge teal\"\u003e🏢 Acid Mine Drainage\u003c\/span\u003e \u003cspan class=\"at441-badge green\"\u003e✓ HiMedia M1963 Equivalent\u003c\/span\u003e \u003cspan class=\"at441-badge amber\"\u003e🇦🇺 Made in Melbourne\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- TWIN STRIP --\u003e\n\u003cdiv class=\"at441-twin\"\u003e\n\u003cdiv class=\"at441-tw\"\u003e\n\u003cdiv class=\"at441-tw-lbl\"\u003eSolution A — Salt Base\u003c\/div\u003e\n\u003cdiv class=\"at441-tw-name\"\u003eInorganic Salts + Wolfe's Mineral\u003c\/div\u003e\n\u003cdiv class=\"at441-tw-desc\"\u003e(NH₄)₂SO₄ + MgSO₄ + K₂HPO₄ + 5 mL Wolfe's Mineral in 800 mL | Adjust pH 2.3 with H₂SO₄ | Filter sterilise 0.22 µm\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-tw\"\u003e\n\u003cdiv class=\"at441-tw-lbl\"\u003eSolution B — Iron Source\u003c\/div\u003e\n\u003cdiv class=\"at441-tw-name\"\u003eFeSO₄·7H₂O — 20 g \/ 200 mL\u003c\/div\u003e\n\u003cdiv class=\"at441-tw-desc\"\u003eDissolve in 200 mL DI water | Filter sterilise IMMEDIATELY | Use within 1 hour | Never store solution\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- CRITICAL BANNER --\u003e\n\u003cdiv class=\"at441-crit\"\u003e\n\u003cstrong\u003e⚠️ DO NOT AUTOCLAVE:\u003c\/strong\u003e \u003cspan\u003eAutoclaving oxidises Fe²⁺ → Fe³⁺, precipitating iron hydroxide and destroying the medium. Filter sterilisation (0.22 µm) only for both solutions.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c!-- SPEC STRIP --\u003e\n\u003cdiv class=\"at441-strip\"\u003e\n\u003cdiv class=\"at441-si\"\u003e\n\u003cdiv class=\"at441-sl\"\u003eCat. No.\u003c\/div\u003e\n\u003cdiv class=\"at441-sv\"\u003eAS-1441\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-si\"\u003e\n\u003cdiv class=\"at441-sl\"\u003eReference\u003c\/div\u003e\n\u003cdiv class=\"at441-sv\"\u003eATCC 2039\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-si\"\u003e\n\u003cdiv class=\"at441-sl\"\u003eFinal pH\u003c\/div\u003e\n\u003cdiv class=\"at441-sv\"\u003e2.3 ± 0.1\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-si\"\u003e\n\u003cdiv class=\"at441-sl\"\u003eSterilisation\u003c\/div\u003e\n\u003cdiv class=\"at441-sv\"\u003e0.22 µm filter\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-si\"\u003e\n\u003cdiv class=\"at441-sl\"\u003eIncubation\u003c\/div\u003e\n\u003cdiv class=\"at441-sv\"\u003e26–30°C + shaking\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- TRUST BAR --\u003e\n\u003cdiv class=\"at441-trust\"\u003e\n\u003cdiv class=\"at441-ti\"\u003e\n\u003cstrong\u003e📄 Full Documentation\u003c\/strong\u003eCOA, TDS \u0026amp; SDS every batch\u003c\/div\u003e\n\u003cdiv class=\"at441-ti\"\u003e\n\u003cstrong\u003e🇦🇺 Australian Stock\u003c\/strong\u003eNo import delays\u003c\/div\u003e\n\u003cdiv class=\"at441-ti\"\u003e\n\u003cstrong\u003e⚡ Same-Week Dispatch\u003c\/strong\u003eMelbourne warehouse\u003c\/div\u003e\n\u003cdiv class=\"at441-ti\"\u003e\n\u003cstrong\u003e🔬 Technical Support\u003c\/strong\u003eDirect from our team\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- TABS CSS-only radio --\u003e \u003cinput class=\"at441-radio\" type=\"radio\" name=\"at441tabs\" id=\"t1\" checked\u003e \u003cinput class=\"at441-radio\" type=\"radio\" name=\"at441tabs\" id=\"t2\"\u003e \u003cinput class=\"at441-radio\" type=\"radio\" name=\"at441tabs\" id=\"t3\"\u003e \u003cinput class=\"at441-radio\" type=\"radio\" name=\"at441tabs\" id=\"t4\"\u003e\n\u003cdiv class=\"at441-tabwrap\"\u003e\n\u003cdiv class=\"at441-tabrow\"\u003e\n\u003clabel for=\"t1\"\u003eProduct Description\u003c\/label\u003e \u003clabel for=\"t2\"\u003eTechnical Data\u003c\/label\u003e \u003clabel for=\"t3\"\u003eSafety Data\u003c\/label\u003e \u003clabel for=\"t4\"\u003eQuality \u0026amp; COA\u003c\/label\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-panels\"\u003e\n\u003c!-- TAB 1 --\u003e\n\u003cdiv id=\"tp1\" class=\"at441-panel\"\u003e\n\u003cdiv class=\"at441-sh\"\u003eOverview\u003c\/div\u003e\n\u003cp style=\"font-size: 14px; color: #374151; margin: 0 0 11px;\"\u003e\u003cstrong\u003eATCC 2039 Broth Twin Pack (AS-1441)\u003c\/strong\u003e is manufactured exactly to the \u003cstrong\u003eofficial ATCC Medium 2039 specification\u003c\/strong\u003e (sourced directly from atcc.org) for the cultivation of \u003cem\u003eAcidithiobacillus ferrooxidans\u003c\/em\u003e ATCC 23270 and related acidophilic chemolithotrophs. The medium is supplied as a twin pack: Solution A (inorganic salts with Wolfe's Mineral Solution incorporated) + Solution B (ferrous sulphate heptahydrate, 20 g per 200 mL).\u003c\/p\u003e\n\u003cp style=\"font-size: 14px; color: #374151; margin: 0 0 11px;\"\u003eThis is the only locally manufactured Australian source for ATCC Medium 2039, supplying the complete official formula including the full Wolfe's Mineral Solution trace element mix. No substitutions or simplifications.\u003c\/p\u003e\n\u003cdiv class=\"at441-sh\"\u003eWhy a Twin Pack?\u003c\/div\u003e\n\u003cdiv class=\"at441-info\"\u003eThe ATCC official protocol specifies that Solution A and Solution B must be prepared and filter-sterilised \u003cstrong\u003eseparately\u003c\/strong\u003e before aseptic combination. This is because:\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e1. FeSO₄ stability:\u003c\/strong\u003e Fe²⁺ oxidises rapidly to Fe³⁺ in the presence of air, moisture, or elevated pH. Storing it with the salt base would cause oxidation before use, precipitating iron as Fe(OH)₃.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e2. DO NOT AUTOCLAVE either solution:\u003c\/strong\u003e Autoclaving at 121°C oxidises Fe²⁺ → Fe³⁺ and precipitates iron hydroxide, destroying the medium's function as an energy source for \u003cem\u003eAcidithiobacillus\u003c\/em\u003e.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. pH adjustment before filter sterilisation:\u003c\/strong\u003e Solution A must be adjusted to pH 2.3 with H₂SO₄ before filter sterilisation — not after.\u003c\/div\u003e\n\u003cdiv class=\"at441-sh\"\u003eWolfe's Mineral Solution — Why It Matters\u003c\/div\u003e\n\u003cdiv class=\"at441-info\"\u003eThe official ATCC 2039 formula includes \u003cstrong\u003e5 mL of Wolfe's Mineral Solution per litre\u003c\/strong\u003e. This is a complete trace element supplement containing 12 components including nitrilotriacetic acid (chelating agent), manganese, cobalt, zinc, copper, aluminium, boron, and molybdenum.\u003cbr\u003e\u003cbr\u003e\u003cem\u003eAcidithiobacillus ferrooxidans\u003c\/em\u003e is an autotroph — it synthesises all organic molecules from CO₂. Without pre-formed amino acids or vitamins from peptone\/yeast extract, it depends entirely on trace minerals supplied in the medium for enzyme cofactors, cell membrane function, and electron transport. Wolfe's Mineral Solution ensures these are present.\u003cbr\u003e\u003cbr\u003eAS-1441 includes the complete Wolfe's Mineral Solution formula incorporated into Solution A — exactly as specified by ATCC.\u003c\/div\u003e\n\u003cdiv class=\"at441-sh\"\u003eApplications\u003c\/div\u003e\n\u003cdiv class=\"at441-apps\"\u003e\n\u003cdiv class=\"at441-app\"\u003e\n\u003cdiv class=\"ico\"\u003e⛏\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eBioleaching R\u0026amp;D\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eCu, Au, U recovery from low-grade ores — heap leach process development\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-app\"\u003e\n\u003cdiv class=\"ico\"\u003e💧\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eAcid Mine Drainage\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eAMD characterisation, bioremediation, iron-oxidising bacteria isolation\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-app\"\u003e\n\u003cdiv class=\"ico\"\u003e🌎\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eEnvironmental\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eAcidophile isolation — mine sites, acid springs, volcanic environments\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-app\"\u003e\n\u003cdiv class=\"ico\"\u003e🧪\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eMining Industry\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eBiohydrometallurgy R\u0026amp;D, mineral sulphide oxidation studies (Rio Tinto, BHP, etc.)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-app\"\u003e\n\u003cdiv class=\"ico\"\u003e📊\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eReference Strain\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eATCC 23270 maintenance and propagation medium\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-app\"\u003e\n\u003cdiv class=\"ico\"\u003e📚\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eResearch \u0026amp; Teaching\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eExtreme environment microbiology, chemolithotrophy demonstration\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-sh\"\u003eFAQs\u003c\/div\u003e\n\u003cdiv class=\"at441-faq\"\u003e\n\u003cdiv class=\"at441-fq\"\u003e\n\u003cdiv class=\"at441-fqq\"\u003e❓ Why must pH be exactly 2.3?\u003c\/div\u003e\n\u003cdiv class=\"at441-fqa\"\u003eAt pH above 3.0, Fe³⁺ precipitates as iron hydroxide Fe(OH)₃ — the orange rust precipitate — and becomes unavailable as an energy source. At pH below 2.0, growth is inhibited. The ATCC protocol specifies pH 2.3 as the target. Verify with a calibrated pH meter before filter sterilisation. Use sterile dilute H₂SO₄ only — NOT HCl, which inhibits iron oxidation in this organism.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-fq\"\u003e\n\u003cdiv class=\"at441-fqq\"\u003e❓ A yellow precipitate forms after combining solutions — is this normal?\u003c\/div\u003e\n\u003cdiv class=\"at441-fqa\"\u003eYes — the ATCC official protocol states: \u003cem\u003e\"A yellow precipitate is normal; it becomes darker as the iron oxidizes.\"\u003c\/em\u003e This is colloidal Fe³⁺ and is expected. The presence of yellow\/orange colour during incubation is actually the growth indicator — it shows that \u003cem\u003eA. ferrooxidans\u003c\/em\u003e is actively oxidising Fe²⁺ to Fe³⁺ as its energy-yielding reaction.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-fq\"\u003e\n\u003cdiv class=\"at441-fqq\"\u003e❓ Why must Solution B be filter-sterilised immediately after dissolution?\u003c\/div\u003e\n\u003cdiv class=\"at441-fqa\"\u003eFeSO₄ solution oxidises rapidly in air — Fe²⁺ becomes Fe³⁺ within hours, precipitating at pH above 2.5. Once Solution B is dissolved, it must be filter-sterilised within 30–60 minutes and combined immediately with sterile Solution A. The ATCC protocol states: \u003cem\u003e\"Stir Solution B to dissolve and quickly filter-sterilize.\"\u003c\/em\u003e Never store prepared Solution B.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-fq\"\u003e\n\u003cdiv class=\"at441-fqq\"\u003e❓ Is AS-1441 equivalent to HiMedia M1963?\u003c\/div\u003e\n\u003cdiv class=\"at441-fqa\"\u003eYes — AS-1441 follows the same official ATCC Medium 2039 specification as HiMedia M1963, including the complete Wolfe's Mineral Solution. AS-1441 is manufactured in Melbourne with same-week dispatch and full Australian batch documentation — no import delays.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-sh\"\u003eCross-Reference \/ Equivalents\u003c\/div\u003e\n\u003ctable class=\"at441-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eSource\u003c\/th\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eCat. No.\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eATCC (official reference)\u003c\/td\u003e\n\u003ctd\u003eATCC Medium 2039 — \u003cem\u003eAcidithiobacillus ferrooxidans\u003c\/em\u003e Medium\u003c\/td\u003e\n\u003ctd\u003eATCC 2039\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHiMedia\u003c\/td\u003e\n\u003ctd\u003eModified 9K Medium Base (Twin Pack)\u003c\/td\u003e\n\u003ctd\u003eM1963\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSilverman \u0026amp; Lundgren (1959)\u003c\/td\u003e\n\u003ctd\u003eOriginal 9K Medium formulation\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c!-- tab 1 --\u003e \u003c!-- TAB 2 --\u003e\n\u003cdiv id=\"tp2\" class=\"at441-panel\"\u003e\n\u003cdiv class=\"at441-sh\"\u003eProduct Specifications\u003c\/div\u003e\n\u003ctable class=\"at441-tbl\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eATCC 2039 Broth (Twin Pack) — Solution A + Solution B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalogue Number\u003c\/td\u003e\n\u003ctd\u003eAS-1441\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReference Standard\u003c\/td\u003e\n\u003ctd\u003eOfficial ATCC Medium 2039 specification (atcc.org) | Silverman \u0026amp; Lundgren 9K Medium (1959)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTarget Organism\u003c\/td\u003e\n\u003ctd\u003e\n\u003cem\u003eAcidithiobacillus ferrooxidans\u003c\/em\u003e ATCC 23270 and related acidophilic chemolithotrophs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePack Format\u003c\/td\u003e\n\u003ctd\u003eTwin Pack — Solution A powder (salts + Wolfe's Mineral) + Solution B powder (FeSO₄·7H₂O) — packaged separately\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSterilisation\u003c\/td\u003e\n\u003ctd class=\"crit\"\u003eFilter sterilise ONLY (0.22 µm membrane) — DO NOT AUTOCLAVE either solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFinal pH\u003c\/td\u003e\n\u003ctd class=\"crit\"\u003e2.3 ± 0.1 (adjust Solution A with sterile dilute H₂SO₄ — verify with calibrated pH meter)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIncubation\u003c\/td\u003e\n\u003ctd\u003e26–30°C, aerobic, shaking 100–150 rpm, 4–14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGrowth indicator\u003c\/td\u003e\n\u003ctd\u003eYellow-orange colour (Fe³⁺ accumulation) — normal per ATCC protocol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf life\u003c\/td\u003e\n\u003ctd\u003e2 years from manufacture in original sealed packaging\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEquivalents\u003c\/td\u003e\n\u003ctd\u003eHiMedia M1963 | ATCC Medium 2039 reference\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"at441-sh\"\u003eSolution A Formula — Per Litre of Final Medium (Official ATCC 2039)\u003c\/div\u003e\n\u003ctable class=\"at441-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredient\u003c\/th\u003e\n\u003cth\u003eAmount\u003c\/th\u003e\n\u003cth\u003eFunction\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eDiammonium Sulphate ((NH₄)₂SO₄)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.8 g\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"key\"\u003eSole nitrogen source for autotrophic growth\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnesium Sulphate Heptahydrate (MgSO₄·7H₂O)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e2.0 g\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEssential macro-mineral; enzyme cofactor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eDipotassium Hydrogen Phosphate (K₂HPO₄)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.4 g\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePhosphorus source; pH buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cstrong\u003eWolfe's Mineral Solution\u003c\/strong\u003e (see below)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e5.0 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"key\"\u003eComplete trace element supplement per ATCC specification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eDI \/ Purified Water\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e800 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAdjust pH to 2.3 with H₂SO₄, then filter sterilise\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"at441-sh\"\u003eWolfe's Mineral Solution — Complete Formula (Official ATCC 2039)\u003c\/div\u003e\n\u003ctable class=\"at441-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredient\u003c\/th\u003e\n\u003cth\u003eg \/ L\u003c\/th\u003e\n\u003cth\u003eNotes\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eNitrilotriacetic acid\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e1.500\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eChelating agent — add first to ~500 mL water, adjust pH 6.5 with KOH to dissolve\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnesium Sulphate Heptahydrate (MgSO₄·7H₂O)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e3.000\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMacro-mineral supplement\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eManganese Sulphate Monohydrate (MnSO₄·H₂O)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.500\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTrace mineral; enzyme cofactor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium Chloride (NaCl)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e1.000\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eIonic balance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eFerrous Sulphate Heptahydrate (FeSO₄·7H₂O)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.100\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTrace iron\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCobalt Chloride Hexahydrate (CoCl₂·6H₂O)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.100\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTrace mineral; vitamin B12 synthesis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eCalcium Chloride (CaCl₂)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.100\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTrace mineral; membrane function\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eZinc Sulphate Heptahydrate (ZnSO₄·7H₂O)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.100\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTrace mineral; enzyme cofactor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eCopper Sulphate Pentahydrate (CuSO₄·5H₂O)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.010\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTrace mineral; cytochrome oxidase\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAluminium Potassium Sulphate (AlK(SO₄)₂·12H₂O)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.010\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTrace mineral\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eBoric Acid (H₃BO₃)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.010\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTrace mineral\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium Molybdate Dihydrate (Na₂MoO₄·2H₂O)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.010\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eTrace mineral; nitrogenase cofactor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eDI \/ Purified Water\u003c\/td\u003e\n\u003ctd\u003eto 1000 mL\u003c\/td\u003e\n\u003ctd\u003eBring to volume after adding all compounds one at a time\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"at441-warn\"\u003eⓘ \u003cstrong\u003eWolfe's Mineral preparation note (from ATCC):\u003c\/strong\u003e Add nitrilotriacetic acid to approximately 500 mL of water and adjust pH to 6.5 with KOH to dissolve the compound. Bring volume to 1000 mL with remaining water and add remaining compounds one at a time.\u003c\/div\u003e\n\u003cdiv class=\"at441-sh\"\u003eSolution B Formula — Per Litre of Final Medium\u003c\/div\u003e\n\u003ctable class=\"at441-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredient\u003c\/th\u003e\n\u003cth\u003eAmount\u003c\/th\u003e\n\u003cth\u003eFunction\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFerrous Sulphate Heptahydrate (FeSO₄·7H₂O)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e20.0 g\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"crit\"\u003ePRIMARY ENERGY SOURCE — A. ferrooxidans oxidises Fe²⁺ → Fe³⁺ as sole energy-yielding reaction. Do NOT reduce or substitute.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eDI \/ Purified Water\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e200 mL\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDissolve and filter-sterilise IMMEDIATELY. Do not store prepared solution.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"at441-sh\"\u003ePreparation Protocol (Official ATCC 2039 Method)\u003c\/div\u003e\n\u003cdiv class=\"at441-danger\"\u003e⚠️ DO NOT AUTOCLAVE either Solution A or Solution B. Autoclaving oxidises Fe²⁺ → Fe³⁺ and precipitates iron hydroxide, making the medium unsuitable for iron-oxidising bacteria.\u003c\/div\u003e\n\u003cdiv class=\"at441-steps\"\u003e\n\u003cdiv class=\"at441-step\"\u003e\n\u003cdiv class=\"at441-stepn\"\u003e1\u003c\/div\u003e\n\u003cdiv class=\"at441-stept\"\u003e\n\u003cstrong\u003ePrepare Wolfe's Mineral Solution:\u003c\/strong\u003e Add nitrilotriacetic acid to ~500 mL DI water, adjust pH to 6.5 with KOH to dissolve. Bring to 1000 mL and add remaining mineral compounds one at a time. Set aside 5.0 mL for Solution A.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-step\"\u003e\n\u003cdiv class=\"at441-stepn\"\u003e2\u003c\/div\u003e\n\u003cdiv class=\"at441-stept\"\u003e\n\u003cstrong\u003ePrepare Solution A:\u003c\/strong\u003e Dissolve (NH₄)₂SO₄ (0.8 g), MgSO₄·7H₂O (2.0 g), and K₂HPO₄ (0.4 g) in 800 mL DI water. Add 5.0 mL Wolfe's Mineral Solution. Mix well.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-step\"\u003e\n\u003cdiv class=\"at441-stepn\"\u003e3\u003c\/div\u003e\n\u003cdiv class=\"at441-stept\"\u003e\n\u003cstrong\u003eAdjust pH of Solution A\u003c\/strong\u003e to 2.3 with sterile dilute H₂SO₄. Verify with calibrated pH meter. Do NOT use HCl.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-step\"\u003e\n\u003cdiv class=\"at441-stepn\"\u003e4\u003c\/div\u003e\n\u003cdiv class=\"at441-stept\"\u003e\n\u003cstrong\u003eFilter sterilise Solution A\u003c\/strong\u003e through 0.22 µm membrane into a sterile vessel.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-step\"\u003e\n\u003cdiv class=\"at441-stepn\"\u003e5\u003c\/div\u003e\n\u003cdiv class=\"at441-stept\"\u003e\n\u003cstrong\u003ePrepare Solution B:\u003c\/strong\u003e Dissolve 20.0 g FeSO₄·7H₂O in 200 mL DI water. Stir to dissolve quickly.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-step\"\u003e\n\u003cdiv class=\"at441-stepn\"\u003e6\u003c\/div\u003e\n\u003cdiv class=\"at441-stept\"\u003e\n\u003cstrong\u003eFilter sterilise Solution B IMMEDIATELY\u003c\/strong\u003e through 0.22 µm membrane. Use within 30–60 minutes of dissolution.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-step\"\u003e\n\u003cdiv class=\"at441-stepn\"\u003e7\u003c\/div\u003e\n\u003cdiv class=\"at441-stept\"\u003e\n\u003cstrong\u003eCombine aseptically:\u003c\/strong\u003e Add sterile Solution B to sterile Solution A. Final volume: 1 litre. A yellow precipitate is normal — it becomes darker as iron oxidises (per ATCC protocol).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-step\"\u003e\n\u003cdiv class=\"at441-stepn\"\u003e8\u003c\/div\u003e\n\u003cdiv class=\"at441-stept\"\u003e\n\u003cstrong\u003eDispense and inoculate.\u003c\/strong\u003e Incubate at 26–30°C aerobically with shaking (100–150 rpm) for 4–14 days.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-sh\"\u003eLiterature References\u003c\/div\u003e\n\u003ctable class=\"at441-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003e#\u003c\/th\u003e\n\u003cth\u003eReference\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003eATCC. Medium 2039: \u003cem\u003eAcidithiobacillus ferrooxidans\u003c\/em\u003e Medium. American Type Culture Collection. atcc.org \u003cstrong\u003e[Primary reference — formula sourced directly from official ATCC PDF]\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003eSilverman MP, Lundgren DG. Studies on the chemoautotrophic iron bacterium \u003cem\u003eFerrobacillus ferrooxidans\u003c\/em\u003e. J Bacteriol. 1959;77(5):642–647. \u003cstrong\u003e[Original 9K medium]\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003ctd\u003eWolin EA, et al. Wolfe's Mineral Solution. J Bacteriol. 1963;81:911–918.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e4\u003c\/td\u003e\n\u003ctd\u003eKelly DP, Wood AP. Reclassification of some species of \u003cem\u003eThiobacillus\u003c\/em\u003e. Int J Syst Evol Microbiol. 2000;50:511–516.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5\u003c\/td\u003e\n\u003ctd\u003eISO 11133:2014. Microbiology of food and water — Performance testing of culture media. Geneva: ISO.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c!-- tab 2 --\u003e \u003c!-- TAB 3 --\u003e\n\u003cdiv id=\"tp3\" class=\"at441-panel\"\u003e\n\u003cdiv class=\"at441-green\"\u003e📄 \u003cstrong\u003eFull 16-section GHS SDS available\u003c\/strong\u003e (Australian WHS Regulations 2023 \/ GHS 7th Edition) — \u003ca href=\"mailto:support@ausamics.com.au\" style=\"color: #2a5240;\"\u003esupport@ausamics.com.au\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-sh\"\u003eSection 1 — Identification\u003c\/div\u003e\n\u003ctable class=\"at441-tbl\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eATCC 2039 Broth Twin Pack (AS-1441) — Solution A + Solution B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended Use\u003c\/td\u003e\n\u003ctd\u003eCulture medium for \u003cem\u003eAcidithiobacillus ferrooxidans\u003c\/em\u003e. Laboratory and research use only.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSupplier\u003c\/td\u003e\n\u003ctd\u003eAuSaMicS Pty Ltd | ABN 56 676 640 467 | 31 Longview CT, Thomastown VIC 3074\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEmergency\u003c\/td\u003e\n\u003ctd\u003ePoisons Information Centre: \u003cstrong\u003e13 11 26\u003c\/strong\u003e (24 hr)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhone\u003c\/td\u003e\n\u003ctd\u003e+61 412 520 598 | support@ausamics.com.au\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"at441-sh\"\u003eSection 2 — Hazard Identification\u003c\/div\u003e\n\u003ctable class=\"at441-tbl\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution A (powder)\u003c\/td\u003e\n\u003ctd\u003eNOT classified as hazardous under Australian WHS Regulations 2023.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution B (FeSO₄·7H₂O powder)\u003c\/td\u003e\n\u003ctd\u003eNOT classified as hazardous at supplied concentrations. Mild irritant in bulk.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrepared medium\u003c\/td\u003e\n\u003ctd class=\"crit\"\u003eCORROSIVE — pH 2.3. GHS05 pictogram. Signal Word: DANGER. H314: severe skin burns and eye damage.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eH₂SO₄ (user-added)\u003c\/td\u003e\n\u003ctd class=\"crit\"\u003eCORROSIVE — handle with acid PPE. Not supplied by AuSaMicS. Consult H₂SO₄ SDS.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrecautionary (prepared)\u003c\/td\u003e\n\u003ctd\u003eP280: wear gloves, eye protection, face shield. P305+P351+P338: if in eyes — rinse 15+ min, seek emergency medical attention. P301+P330+P331: if ingested — do not induce vomiting, call 13 11 26.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"at441-sh\"\u003eKey Components — Section 3\u003c\/div\u003e\n\u003ctable class=\"at441-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eCAS\u003c\/th\u003e\n\u003cth\u003eSolution\u003c\/th\u003e\n\u003cth\u003eHazard\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e(NH₄)₂SO₄\u003c\/td\u003e\n\u003ctd\u003e7783-20-2\u003c\/td\u003e\n\u003ctd\u003eA\u003c\/td\u003e\n\u003ctd\u003eNot hazardous at use level\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMgSO₄·7H₂O\u003c\/td\u003e\n\u003ctd\u003e10034-99-8\u003c\/td\u003e\n\u003ctd\u003eA + Wolfe's\u003c\/td\u003e\n\u003ctd\u003eNot hazardous at use level\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eK₂HPO₄\u003c\/td\u003e\n\u003ctd\u003e7758-11-4\u003c\/td\u003e\n\u003ctd\u003eA\u003c\/td\u003e\n\u003ctd\u003eMild irritant at high concentration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNitrilotriacetic acid\u003c\/td\u003e\n\u003ctd\u003e139-13-9\u003c\/td\u003e\n\u003ctd\u003eWolfe's\u003c\/td\u003e\n\u003ctd\u003eIrritant; possible reproductive toxicant at high doses — use PPE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFeSO₄·7H₂O\u003c\/td\u003e\n\u003ctd\u003e7782-63-0\u003c\/td\u003e\n\u003ctd\u003eB + Wolfe's\u003c\/td\u003e\n\u003ctd\u003eIrritant in powder; corrosive medium at pH 2.3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eH₂SO₄ (user-added)\u003c\/td\u003e\n\u003ctd\u003e7664-93-9\u003c\/td\u003e\n\u003ctd\u003eUser adds to A\u003c\/td\u003e\n\u003ctd\u003eCORROSIVE — not supplied\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"at441-sh\"\u003eFirst Aid \u0026amp; PPE Summary\u003c\/div\u003e\n\u003ctable class=\"at441-tbl\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSkin (powder)\u003c\/td\u003e\n\u003ctd\u003eWash soap and water 15 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSkin (prepared medium)\u003c\/td\u003e\n\u003ctd\u003eFlush with large amounts of water 20 min. Seek medical attention.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEyes (prepared medium)\u003c\/td\u003e\n\u003ctd\u003eImmediately rinse 15+ min. Emergency medical attention. pH 2.3 is corrosive.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIngestion\u003c\/td\u003e\n\u003ctd\u003eDo NOT induce vomiting. Call 13 11 26 immediately.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePPE — powder\u003c\/td\u003e\n\u003ctd\u003eSafety glasses, nitrile gloves, lab coat, P1 respirator for bulk weighing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePPE — prepared medium\u003c\/td\u003e\n\u003ctd\u003eChemical splash goggles, acid-resistant gloves, lab coat — mandatory\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpill (prepared)\u003c\/td\u003e\n\u003ctd\u003eNeutralise with NaHCO₃. Prevent drain entry — pH 2.3 harmful to aquatic environment.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDisposal\u003c\/td\u003e\n\u003ctd\u003eNeutralise to pH 6–8. Iron hydroxide precipitate as inorganic waste per local EPA requirements.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTransport\u003c\/td\u003e\n\u003ctd\u003ePowder: not DG. Prepared acidic medium: verify corrosive liquid classification before transport.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c!-- tab 3 --\u003e \u003c!-- TAB 4 --\u003e\n\u003cdiv id=\"tp4\" class=\"at441-panel\"\u003e\n\u003cdiv class=\"at441-sh\"\u003eQuality Specifications\u003c\/div\u003e\n\u003ctable class=\"at441-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eTypical Result\u003c\/th\u003e\n\u003cth\u003eMethod\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution A appearance\u003c\/td\u003e\n\u003ctd\u003eWhite to off-white free-flowing powder\u003c\/td\u003e\n\u003ctd\u003eConforms\u003c\/td\u003e\n\u003ctd\u003eVisual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution B appearance\u003c\/td\u003e\n\u003ctd\u003ePale green to blue-green crystalline powder\u003c\/td\u003e\n\u003ctd\u003eConforms\u003c\/td\u003e\n\u003ctd\u003eVisual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution B purity (FeSO₄·7H₂O)\u003c\/td\u003e\n\u003ctd\u003e≥ 99.0%\u003c\/td\u003e\n\u003ctd\u003e99.4%\u003c\/td\u003e\n\u003ctd\u003eTitration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution B iron content (as Fe)\u003c\/td\u003e\n\u003ctd\u003e19.0 – 20.0%\u003c\/td\u003e\n\u003ctd\u003e19.7%\u003c\/td\u003e\n\u003ctd\u003eAAS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFinal pH (prepared, 25°C)\u003c\/td\u003e\n\u003ctd\u003e2.3 ± 0.1\u003c\/td\u003e\n\u003ctd\u003e2.3\u003c\/td\u003e\n\u003ctd\u003epH meter (calibrated)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution A moisture\u003c\/td\u003e\n\u003ctd\u003e≤ 3.0%\u003c\/td\u003e\n\u003ctd\u003e1.4%\u003c\/td\u003e\n\u003ctd\u003eLoss on drying\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGrowth — \u003cem\u003eA. ferrooxidans\u003c\/em\u003e ATCC 23270\u003c\/td\u003e\n\u003ctd\u003eGrowth + yellow-orange colour by day 7–14 at 26–30°C\u003c\/td\u003e\n\u003ctd\u003eGrowth confirmed\u003c\/td\u003e\n\u003ctd\u003eQC organism test\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSterility (uninoculated)\u003c\/td\u003e\n\u003ctd\u003eNo growth at 30°C \/ 7 days\u003c\/td\u003e\n\u003ctd\u003eNo growth\u003c\/td\u003e\n\u003ctd\u003eSterility check\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBatch COA\u003c\/td\u003e\n\u003ctd\u003eLot-specific for both Solution A and Solution B\u003c\/td\u003e\n\u003ctd\u003eIncluded every order\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"at441-sh\"\u003ePack Sizes\u003c\/div\u003e\n\u003ctable class=\"at441-tbl\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e10L Twin Pack\u003c\/td\u003e\n\u003ctd\u003eSolution A: ~33 g | Solution B: 200 g | Makes 10 litres prepared medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003e\u003cstrong\u003e25L Twin Pack ⭐\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSolution A: ~82 g | Solution B: 500 g | Makes 25 litres prepared medium — most popular\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCustom\u003c\/td\u003e\n\u003ctd\u003eContact support@ausamics.com.au for bulk or project-specific quantities\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"at441-sh\"\u003eManufacturing \u0026amp; Documentation\u003c\/div\u003e\n\u003cdiv class=\"at441-green\"\u003e\n\u003cstrong\u003eManufactured by AuSaMicS Pty Ltd\u003c\/strong\u003e\u003cbr\u003e31 Longview CT, Thomastown VIC 3074, Australia | ABN 56 676 640 467\u003cbr\u003e\u003cbr\u003e✓ Formulated exactly to official ATCC Medium 2039 specification (atcc.org)\u003cbr\u003e✓ Complete Wolfe's Mineral Solution (12 components) incorporated in Solution A\u003cbr\u003e✓ Solution B: FeSO₄·7H₂O ≥99.0% — lot-specific iron content on COA\u003cbr\u003e✓ COA, TDS, SDS included with every order\u003cbr\u003e✓ Australian stock — same-week dispatch, no import delays\u003cbr\u003e✓ Only locally manufactured ATCC 2039 in Australia\u003c\/div\u003e\n\u003cdiv class=\"at441-danger\"\u003e⚠️ For laboratory and research use only. Prepared medium is corrosive (pH 2.3). Acid-resistant gloves and chemical splash goggles mandatory. Not for food, therapeutic, veterinary, or household use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- tab 4 --\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"at441-footer\"\u003e\n\u003cstrong\u003eAuSaMicS Pty Ltd\u003c\/strong\u003e | 31 Longview CT, Thomastown VIC 3074, Australia | ABN 56 676 640 467\u003cbr\u003esupport@ausamics.com.au | +61 412 520 598 | www.ausamics.com.au\u003cbr\u003e\u003cspan style=\"color: #94a3b8;\"\u003eFor laboratory and research use only. The information provided is accurate to the best of our knowledge at the time of publication. AuSaMicS Pty Ltd accepts no liability for any loss, damage, or injury arising from use outside the intended application.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"AuSaMicS","offers":[{"title":"Default Title","offer_id":48875085627647,"sku":"AS-1441-500G","price":125.0,"currency_code":"AUD","in_stock":true}],"url":"https:\/\/ausamics.com.au\/products\/atcc-2039-broth-twin-pack","provider":"AuSaMicS","version":"1.0","type":"link"}