{"product_id":"azide-dextrose-agar-rothe-ada","title":"Azide Dextrose Agar (ROTHE)","description":"\u003ch3 data-start=\"554\" data-end=\"591\"\u003eAzide Dextrose Agar (ROTHE) – ADA\u003c\/h3\u003e\n\u003cp data-start=\"593\" data-end=\"691\"\u003eSelective Medium for Enterococci Detection in Water, Food, and Dairy\u003cbr data-start=\"661\" data-end=\"664\"\u003eCatalog Number: \u003cstrong data-start=\"680\" data-end=\"691\"\u003eAS-1127\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"693\" data-end=\"696\"\u003e\n\u003ch4 data-start=\"698\" data-end=\"711\"\u003eOverview\u003c\/h4\u003e\n\u003cp data-start=\"713\" data-end=\"1062\"\u003eAzide Dextrose Agar (ROTHE), also known as \u003cstrong data-start=\"756\" data-end=\"771\"\u003eRoth’s Agar\u003c\/strong\u003e or \u003cstrong data-start=\"775\" data-end=\"816\"\u003eAzide Blood Agar Base (without blood)\u003c\/strong\u003e, is a highly selective solid culture medium originally developed by \u003cstrong data-start=\"885\" data-end=\"908\"\u003eRothe et al. (1954)\u003c\/strong\u003e for the isolation and enumeration of \u003cstrong data-start=\"946\" data-end=\"983\"\u003efaecal streptococci \/ enterococci\u003c\/strong\u003e, including \u003cem data-start=\"995\" data-end=\"1019\"\u003eStreptococcus faecalis\u003c\/em\u003e, \u003cem data-start=\"1021\" data-end=\"1033\"\u003eS. faecium\u003c\/em\u003e, and \u003cem data-start=\"1039\" data-end=\"1053\"\u003eEnterococcus\u003c\/em\u003e species.\u003c\/p\u003e\n\u003cp data-start=\"1064\" data-end=\"1335\"\u003eThe inclusion of \u003cstrong data-start=\"1081\" data-end=\"1111\"\u003esodium azide (0.02–0.04 %)\u003c\/strong\u003e strongly inhibits Gram-negative bacteria and most competing Gram-positive flora, while enterococci grow readily. Typical colonies appear \u003cstrong data-start=\"1249\" data-end=\"1283\"\u003esmall, translucent to pink-red\u003c\/strong\u003e, allowing reliable differentiation and enumeration.\u003c\/p\u003e\n\u003cp data-start=\"1337\" data-end=\"1506\"\u003eAzide Dextrose Agar is widely used in \u003cstrong data-start=\"1375\" data-end=\"1414\"\u003ewater, food, and dairy microbiology\u003c\/strong\u003e, including methods aligned with \u003cstrong data-start=\"1447\" data-end=\"1461\"\u003eISO 7899-2\u003c\/strong\u003e for the detection of intestinal enterococci.\u003c\/p\u003e\n\u003chr data-start=\"1508\" data-end=\"1511\"\u003e\n\u003ch4 data-start=\"1513\" data-end=\"1530\"\u003eApplications\u003c\/h4\u003e\n\u003cul data-start=\"1532\" data-end=\"1903\"\u003e\n\u003cli data-start=\"1532\" data-end=\"1650\"\u003e\n\u003cp data-start=\"1534\" data-end=\"1650\"\u003eDetection and enumeration of enterococci in drinking, bathing, and waste water\u003cbr data-start=\"1612\" data-end=\"1615\"\u003e\u003cem data-start=\"1617\" data-end=\"1648\"\u003e(ISO 7899-2 reference method)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1651\" data-end=\"1705\"\u003e\n\u003cp data-start=\"1653\" data-end=\"1705\"\u003eQuality-control testing of milk and dairy products\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1706\" data-end=\"1782\"\u003e\n\u003cp data-start=\"1708\" data-end=\"1782\"\u003eIsolation of enterococci from foods such as meat, cheese, and vegetables\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1783\" data-end=\"1903\"\u003e\n\u003cp data-start=\"1785\" data-end=\"1903\"\u003eConfirmation of enterococci following presumptive growth on\u003cbr data-start=\"1844\" data-end=\"1847\"\u003em-Enterococcus Agar or Kanamycin-Aesculin-Azide Agar\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"1905\" data-end=\"1908\"\u003e\n\u003ch4 data-start=\"1910\" data-end=\"1938\"\u003eKey Features \u0026amp; Benefits\u003c\/h4\u003e\n\u003cul data-start=\"1940\" data-end=\"2262\"\u003e\n\u003cli data-start=\"1940\" data-end=\"1994\"\u003e\n\u003cp data-start=\"1942\" data-end=\"1994\"\u003eHigh selectivity due to sodium azide (0.02–0.04 %)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1995\" data-end=\"2077\"\u003e\n\u003cp data-start=\"1997\" data-end=\"2077\"\u003eGlucose content (0.5–1 %) enhances characteristic pink-red colony pigmentation\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2078\" data-end=\"2189\"\u003e\n\u003cp data-start=\"2080\" data-end=\"2189\"\u003eCan be used with or without \u003cstrong data-start=\"2108\" data-end=\"2129\"\u003e5 % sterile blood\u003c\/strong\u003e\u003cbr data-start=\"2129\" data-end=\"2132\"\u003e\u003cem data-start=\"2134\" data-end=\"2187\"\u003e(blood supplementation improves colony distinction)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2190\" data-end=\"2262\"\u003e\n\u003cp data-start=\"2192\" data-end=\"2262\"\u003eEstablished medium in European water and food microbiology protocols\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"2264\" data-end=\"2267\"\u003e\n\u003ch4 data-start=\"2269\" data-end=\"2305\"\u003eTypical Composition (per litre)\u003c\/h4\u003e\n\u003cul data-start=\"2307\" data-end=\"2450\"\u003e\n\u003cli data-start=\"2307\" data-end=\"2345\"\u003e\n\u003cp data-start=\"2309\" data-end=\"2345\"\u003ePeptone (or meat extract) – 19.5 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2346\" data-end=\"2365\"\u003e\n\u003cp data-start=\"2348\" data-end=\"2365\"\u003eGlucose – 7.5 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2366\" data-end=\"2400\"\u003e\n\u003cp data-start=\"2368\" data-end=\"2400\"\u003eSodium chloride (NaCl) – 7.5 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2401\" data-end=\"2432\"\u003e\n\u003cp data-start=\"2403\" data-end=\"2432\"\u003eSodium azide (NaN₃) – 0.2 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2433\" data-end=\"2450\"\u003e\n\u003cp data-start=\"2435\" data-end=\"2450\"\u003eAgar – 15.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2452\" data-end=\"2484\"\u003eFinal pH: \u003cstrong data-start=\"2462\" data-end=\"2484\"\u003e7.2 ± 0.2 at 25 °C\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"2486\" data-end=\"2489\"\u003e\n\u003ch4 data-start=\"2491\" data-end=\"2507\"\u003ePreparation\u003c\/h4\u003e\n\u003cul data-start=\"2509\" data-end=\"2909\"\u003e\n\u003cli data-start=\"2509\" data-end=\"2608\"\u003e\n\u003cp data-start=\"2511\" data-end=\"2608\"\u003eSuspend \u003cstrong data-start=\"2519\" data-end=\"2529\"\u003e47.9 g\u003c\/strong\u003e of dehydrated medium in \u003cstrong data-start=\"2554\" data-end=\"2561\"\u003e1 L\u003c\/strong\u003e purified water\u003cbr data-start=\"2576\" data-end=\"2579\"\u003e\u003cem data-start=\"2581\" data-end=\"2606\"\u003e(manufacturer-specific)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2609\" data-end=\"2689\"\u003e\n\u003cp data-start=\"2611\" data-end=\"2689\"\u003eHeat with frequent agitation and boil for \u003cstrong data-start=\"2653\" data-end=\"2665\"\u003e1 minute\u003c\/strong\u003e until fully dissolved\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2690\" data-end=\"2754\"\u003e\n\u003cp data-start=\"2692\" data-end=\"2754\"\u003e\u003cstrong data-start=\"2692\" data-end=\"2712\"\u003eDo not autoclave\u003c\/strong\u003e (sodium azide is heat-labile and toxic)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2755\" data-end=\"2779\"\u003e\n\u003cp data-start=\"2757\" data-end=\"2779\"\u003eCool to \u003cstrong data-start=\"2765\" data-end=\"2777\"\u003e45–50 °C\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2780\" data-end=\"2874\"\u003e\n\u003cp data-start=\"2782\" data-end=\"2874\"\u003eOptional: add \u003cstrong data-start=\"2796\" data-end=\"2832\"\u003e5–7 % sterile defibrinated blood\u003c\/strong\u003e to prepare\u003cbr data-start=\"2843\" data-end=\"2846\"\u003eAzide Blood Agar (ROTHE)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2875\" data-end=\"2909\"\u003e\n\u003cp data-start=\"2877\" data-end=\"2909\"\u003ePour into sterile Petri dishes\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"2911\" data-end=\"2914\"\u003e\n\u003ch4 data-start=\"2916\" data-end=\"2942\"\u003eIncubation Conditions\u003c\/h4\u003e\n\u003cul data-start=\"2944\" data-end=\"3069\"\u003e\n\u003cli data-start=\"2944\" data-end=\"2973\"\u003e\n\u003cp data-start=\"2946\" data-end=\"2973\"\u003e\u003cstrong data-start=\"2946\" data-end=\"2962\"\u003eTemperature:\u003c\/strong\u003e 35–37 °C\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2974\" data-end=\"2999\"\u003e\n\u003cp data-start=\"2976\" data-end=\"2999\"\u003e\u003cstrong data-start=\"2976\" data-end=\"2985\"\u003eTime:\u003c\/strong\u003e 24–48 hours\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3000\" data-end=\"3069\"\u003e\n\u003cp data-start=\"3002\" data-end=\"3069\"\u003e\u003cstrong data-start=\"3002\" data-end=\"3024\"\u003eColony morphology:\u003c\/strong\u003e 0.5–2 mm, translucent to pink-red colonies\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3071\" data-end=\"3074\"\u003e\n\u003ch4 data-start=\"3076\" data-end=\"3100\"\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cul data-start=\"3102\" data-end=\"3288\"\u003e\n\u003cli data-start=\"3102\" data-end=\"3180\"\u003e\n\u003cp data-start=\"3104\" data-end=\"3180\"\u003e\u003cstrong data-start=\"3104\" data-end=\"3126\"\u003eDehydrated medium:\u003c\/strong\u003e Store below \u003cstrong data-start=\"3139\" data-end=\"3148\"\u003e25 °C\u003c\/strong\u003e, dry and protected from light\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3181\" data-end=\"3247\"\u003e\n\u003cp data-start=\"3183\" data-end=\"3247\"\u003e\u003cstrong data-start=\"3183\" data-end=\"3203\"\u003ePrepared plates:\u003c\/strong\u003e Store at \u003cstrong data-start=\"3213\" data-end=\"3223\"\u003e2–8 °C\u003c\/strong\u003e, protected from light\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3248\" data-end=\"3288\"\u003e\n\u003cp data-start=\"3250\" data-end=\"3288\"\u003eRecommended use within \u003cstrong data-start=\"3273\" data-end=\"3286\"\u003e2–3 weeks\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3290\" data-end=\"3293\"\u003e\n\u003ch4 data-start=\"3295\" data-end=\"3311\"\u003eSafety Note\u003c\/h4\u003e\n\u003cp data-start=\"3313\" data-end=\"3517\"\u003eSodium azide is \u003cstrong data-start=\"3329\" data-end=\"3345\"\u003ehighly toxic\u003c\/strong\u003e and may form \u003cstrong data-start=\"3359\" data-end=\"3382\"\u003eexplosive compounds\u003c\/strong\u003e in contact with metals.\u003cbr data-start=\"3406\" data-end=\"3409\"\u003eDispose of prepared plates and waste in accordance with laboratory safety and chemical disposal regulations.\u003c\/p\u003e\n\u003chr data-start=\"3519\" data-end=\"3522\"\u003e\n\u003ch4 data-start=\"3524\" data-end=\"3541\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp data-start=\"3543\" data-end=\"3676\"\u003eFor laboratory and water\/food microbiology use only.\u003cbr data-start=\"3595\" data-end=\"3598\"\u003eNot for diagnostic or therapeutic applications without appropriate validation.\u003c\/p\u003e\n\u003chr data-start=\"3678\" data-end=\"3681\"\u003e\n\u003ch4 data-start=\"3683\" data-end=\"3708\"\u003eQuality \u0026amp; Compliance\u003c\/h4\u003e\n\u003cul data-start=\"3710\" data-end=\"3906\"\u003e\n\u003cli data-start=\"3710\" data-end=\"3772\"\u003e\n\u003cp data-start=\"3712\" data-end=\"3772\"\u003eSelective medium for enterococci detection and enumeration\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3773\" data-end=\"3839\"\u003e\n\u003cp data-start=\"3775\" data-end=\"3839\"\u003eSuitable for ISO-aligned water and food microbiology workflows\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3840\" data-end=\"3906\"\u003e\n\u003cp data-start=\"3842\" data-end=\"3906\"\u003eManufactured under controlled conditions for batch consistency\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3908\" data-end=\"3911\"\u003e\n\u003ch4 data-start=\"3913\" data-end=\"3945\"\u003eCustoms \u0026amp; Trade Information\u003c\/h4\u003e\n\u003cp data-start=\"3947\" data-end=\"4052\"\u003eHS \/ AHECC Code: \u003cstrong data-start=\"3964\" data-end=\"3978\"\u003e3821.00.00\u003c\/strong\u003e\u003cbr data-start=\"3978\" data-end=\"3981\"\u003ePrepared culture media for development or maintenance of microorganisms\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"100G","offer_id":48052835942655,"sku":"AS-1127-DH-LS-100G","price":45.0,"currency_code":"AUD","in_stock":true},{"title":"500G","offer_id":48052835975423,"sku":"AS-1127-DH-LS-500G","price":115.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_174fae9f-31ab-44ac-9728-fa1b4149b3f3.jpg?v=1771063026","url":"https:\/\/ausamics.com.au\/products\/azide-dextrose-agar-rothe-ada","provider":"AuSaMicS","version":"1.0","type":"link"}