{"product_id":"azide-dextrose-broth-rothe-adb","title":"Azide Dextrose Broth (ROTHE)","description":"\u003ch3 data-start=\"532\" data-end=\"570\"\u003eAzide Dextrose Broth (ROTHE) – ADB\u003c\/h3\u003e\n\u003cp data-start=\"572\" data-end=\"681\"\u003eSelective Enrichment Medium for Enterococci Detection in Water, Food, and Dairy\u003cbr data-start=\"651\" data-end=\"654\"\u003eCatalog Number: \u003cstrong data-start=\"670\" data-end=\"681\"\u003eAS-1128\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"683\" data-end=\"686\"\u003e\n\u003ch4 data-start=\"688\" data-end=\"701\"\u003eOverview\u003c\/h4\u003e\n\u003cp data-start=\"703\" data-end=\"1033\"\u003eAzide Dextrose Broth (ROTHE) is a \u003cstrong data-start=\"737\" data-end=\"782\"\u003ehighly selective liquid enrichment medium\u003c\/strong\u003e originally developed by \u003cstrong data-start=\"807\" data-end=\"830\"\u003eRothe et al. (1954)\u003c\/strong\u003e for the isolation and presumptive detection of \u003cstrong data-start=\"878\" data-end=\"915\"\u003efaecal streptococci \/ enterococci\u003c\/strong\u003e, including \u003cem data-start=\"927\" data-end=\"950\"\u003eEnterococcus faecalis\u003c\/em\u003e, \u003cem data-start=\"952\" data-end=\"964\"\u003eE. faecium\u003c\/em\u003e, and \u003cem data-start=\"970\" data-end=\"981\"\u003eE. durans\u003c\/em\u003e, from water, milk, food, and environmental samples.\u003c\/p\u003e\n\u003cp data-start=\"1035\" data-end=\"1437\"\u003eThe presence of \u003cstrong data-start=\"1051\" data-end=\"1081\"\u003esodium azide (0.02–0.04 %)\u003c\/strong\u003e inhibits Gram-negative bacteria and most competing Gram-positive flora, while enterococci grow rapidly, producing \u003cstrong data-start=\"1196\" data-end=\"1243\"\u003edense turbidity within 18–24 hours at 37 °C\u003c\/strong\u003e.\u003cbr data-start=\"1244\" data-end=\"1247\"\u003eAzide Dextrose Broth is specified as the \u003cstrong data-start=\"1288\" data-end=\"1316\"\u003eofficial enrichment step\u003c\/strong\u003e in \u003cstrong data-start=\"1320\" data-end=\"1334\"\u003eISO 7899-1\u003c\/strong\u003e and \u003cstrong data-start=\"1339\" data-end=\"1352\"\u003eDIN 38411\u003c\/strong\u003e prior to confirmation on bile-aesculin-azide agar or by membrane filtration methods.\u003c\/p\u003e\n\u003chr data-start=\"1439\" data-end=\"1442\"\u003e\n\u003ch4 data-start=\"1444\" data-end=\"1461\"\u003eApplications\u003c\/h4\u003e\n\u003cul data-start=\"1463\" data-end=\"1858\"\u003e\n\u003cli data-start=\"1463\" data-end=\"1579\"\u003e\n\u003cp data-start=\"1465\" data-end=\"1579\"\u003eSelective enrichment of enterococci from heavily contaminated samples\u003cbr data-start=\"1534\" data-end=\"1537\"\u003e\u003cem data-start=\"1539\" data-end=\"1577\"\u003e(water, sewage, milk, meat products)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1580\" data-end=\"1664\"\u003e\n\u003cp data-start=\"1582\" data-end=\"1664\"\u003eMost Probable Number (MPN) determination of faecal streptococci in water testing\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1665\" data-end=\"1758\"\u003e\n\u003cp data-start=\"1667\" data-end=\"1758\"\u003eFirst enrichment step in ISO 7899-1 and national standards for bathing and drinking water\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1759\" data-end=\"1858\"\u003e\n\u003cp data-start=\"1761\" data-end=\"1858\"\u003ePre-enrichment prior to confirmation on\u003cbr data-start=\"1800\" data-end=\"1803\"\u003eKanamycin-Aesculin-Azide Agar or Bile Aesculin Agar\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"1860\" data-end=\"1863\"\u003e\n\u003ch4 data-start=\"1865\" data-end=\"1893\"\u003eKey Features \u0026amp; Benefits\u003c\/h4\u003e\n\u003cul data-start=\"1895\" data-end=\"2217\"\u003e\n\u003cli data-start=\"1895\" data-end=\"1976\"\u003e\n\u003cp data-start=\"1897\" data-end=\"1976\"\u003eHigh selectivity due to sodium azide inhibition\u003cbr data-start=\"1944\" data-end=\"1947\"\u003e\u003cem data-start=\"1949\" data-end=\"1974\"\u003e(typically 0.2–0.3 g\/L)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1977\" data-end=\"2060\"\u003e\n\u003cp data-start=\"1979\" data-end=\"2060\"\u003eRapid and heavy growth of enterococci with visible turbidity within 18–24 hours\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2061\" data-end=\"2128\"\u003e\n\u003cp data-start=\"2063\" data-end=\"2128\"\u003eAvailable in single- and double-strength formats for MPN series\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2129\" data-end=\"2217\"\u003e\n\u003cp data-start=\"2131\" data-end=\"2217\"\u003ePositive tubes show dense turbidity with sediment, requiring confirmatory subculture\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"2219\" data-end=\"2222\"\u003e\n\u003ch4 data-start=\"2224\" data-end=\"2279\"\u003eStandard Composition (per litre – single strength)\u003c\/h4\u003e\n\u003cul data-start=\"2281\" data-end=\"2502\"\u003e\n\u003cli data-start=\"2281\" data-end=\"2316\"\u003e\n\u003cp data-start=\"2283\" data-end=\"2316\"\u003ePeptone \/ meat extract – 20.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2317\" data-end=\"2347\"\u003e\n\u003cp data-start=\"2319\" data-end=\"2347\"\u003eGlucose (dextrose) – 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2348\" data-end=\"2382\"\u003e\n\u003cp data-start=\"2350\" data-end=\"2382\"\u003eSodium chloride (NaCl) – 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2383\" data-end=\"2425\"\u003e\n\u003cp data-start=\"2385\" data-end=\"2425\"\u003eDipotassium phosphate (K₂HPO₄) – 2.7 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2426\" data-end=\"2470\"\u003e\n\u003cp data-start=\"2428\" data-end=\"2470\"\u003eMonopotassium phosphate (KH₂PO₄) – 2.7 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2471\" data-end=\"2502\"\u003e\n\u003cp data-start=\"2473\" data-end=\"2502\"\u003eSodium azide (NaN₃) – 0.2 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2504\" data-end=\"2536\"\u003eFinal pH: \u003cstrong data-start=\"2514\" data-end=\"2536\"\u003e6.8 ± 0.2 at 25 °C\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"2538\" data-end=\"2541\"\u003e\n\u003ch4 data-start=\"2543\" data-end=\"2559\"\u003ePreparation\u003c\/h4\u003e\n\u003cul data-start=\"2561\" data-end=\"3018\"\u003e\n\u003cli data-start=\"2561\" data-end=\"2632\"\u003e\n\u003cp data-start=\"2563\" data-end=\"2632\"\u003eDissolve \u003cstrong data-start=\"2572\" data-end=\"2583\"\u003e32–35 g\u003c\/strong\u003e of dehydrated medium in \u003cstrong data-start=\"2608\" data-end=\"2615\"\u003e1 L\u003c\/strong\u003e purified water\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2633\" data-end=\"2692\"\u003e\n\u003cp data-start=\"2635\" data-end=\"2692\"\u003eHeat with frequent agitation until completely dissolved\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2693\" data-end=\"2757\"\u003e\n\u003cp data-start=\"2695\" data-end=\"2757\"\u003e\u003cstrong data-start=\"2695\" data-end=\"2715\"\u003eDo not autoclave\u003c\/strong\u003e (sodium azide is heat-labile and toxic)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2758\" data-end=\"2882\"\u003e\n\u003cp data-start=\"2760\" data-end=\"2782\"\u003eDispense into tubes:\u003c\/p\u003e\n\u003cul data-start=\"2785\" data-end=\"2882\"\u003e\n\u003cli data-start=\"2785\" data-end=\"2824\"\u003e\n\u003cp data-start=\"2787\" data-end=\"2824\"\u003e\u003cstrong data-start=\"2787\" data-end=\"2807\"\u003eSingle strength:\u003c\/strong\u003e 10 mL per tube\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2827\" data-end=\"2882\"\u003e\n\u003cp data-start=\"2829\" data-end=\"2882\"\u003e\u003cstrong data-start=\"2829\" data-end=\"2849\"\u003eDouble strength:\u003c\/strong\u003e 5 mL per tube (for MPN series)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2883\" data-end=\"3018\"\u003e\n\u003cp data-start=\"2885\" data-end=\"3018\"\u003eSterilise by \u003cstrong data-start=\"2898\" data-end=\"2916\"\u003etyndallisation\u003c\/strong\u003e\u003cbr data-start=\"2916\" data-end=\"2919\"\u003e\u003cem data-start=\"2921\" data-end=\"2971\"\u003e(100 °C for 30 minutes on three successive days)\u003c\/em\u003e\u003cbr data-start=\"2971\" data-end=\"2974\"\u003eor by membrane filtration where required\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3020\" data-end=\"3023\"\u003e\n\u003ch4 data-start=\"3025\" data-end=\"3051\"\u003eIncubation Conditions\u003c\/h4\u003e\n\u003cul data-start=\"3053\" data-end=\"3235\"\u003e\n\u003cli data-start=\"3053\" data-end=\"3145\"\u003e\n\u003cp data-start=\"3055\" data-end=\"3145\"\u003e\u003cstrong data-start=\"3055\" data-end=\"3071\"\u003eTemperature:\u003c\/strong\u003e 35–37 °C\u003cbr data-start=\"3080\" data-end=\"3083\"\u003e\u003cem data-start=\"3085\" data-end=\"3143\"\u003e(44 °C may be used for faecal streptococci confirmation)\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3146\" data-end=\"3171\"\u003e\n\u003cp data-start=\"3148\" data-end=\"3171\"\u003e\u003cstrong data-start=\"3148\" data-end=\"3157\"\u003eTime:\u003c\/strong\u003e 18–48 hours\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3172\" data-end=\"3235\"\u003e\n\u003cp data-start=\"3174\" data-end=\"3235\"\u003e\u003cstrong data-start=\"3174\" data-end=\"3196\"\u003ePositive reaction:\u003c\/strong\u003e Dense turbidity, often with sediment\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3237\" data-end=\"3240\"\u003e\n\u003ch4 data-start=\"3242\" data-end=\"3266\"\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cul data-start=\"3268\" data-end=\"3429\"\u003e\n\u003cli data-start=\"3268\" data-end=\"3346\"\u003e\n\u003cp data-start=\"3270\" data-end=\"3346\"\u003e\u003cstrong data-start=\"3270\" data-end=\"3292\"\u003eDehydrated medium:\u003c\/strong\u003e Store at \u003cstrong data-start=\"3302\" data-end=\"3314\"\u003e10–30 °C\u003c\/strong\u003e, dry and protected from light\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3347\" data-end=\"3390\"\u003e\n\u003cp data-start=\"3349\" data-end=\"3390\"\u003e\u003cstrong data-start=\"3349\" data-end=\"3368\"\u003ePrepared broth:\u003c\/strong\u003e Store at \u003cstrong data-start=\"3378\" data-end=\"3388\"\u003e2–8 °C\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3391\" data-end=\"3429\"\u003e\n\u003cp data-start=\"3393\" data-end=\"3429\"\u003eRecommended use within \u003cstrong data-start=\"3416\" data-end=\"3427\"\u003e4 weeks\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3431\" data-end=\"3434\"\u003e\n\u003ch4 data-start=\"3436\" data-end=\"3452\"\u003eSafety Note\u003c\/h4\u003e\n\u003cp data-start=\"3454\" data-end=\"3661\"\u003eSodium azide is \u003cstrong data-start=\"3470\" data-end=\"3486\"\u003ehighly toxic\u003c\/strong\u003e and may form \u003cstrong data-start=\"3500\" data-end=\"3523\"\u003eexplosive compounds\u003c\/strong\u003e in contact with copper or lead plumbing.\u003cbr data-start=\"3564\" data-end=\"3567\"\u003eDispose of broth and contaminated materials according to laboratory chemical-safety protocols.\u003c\/p\u003e\n\u003chr data-start=\"3663\" data-end=\"3666\"\u003e\n\u003ch4 data-start=\"3668\" data-end=\"3685\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp data-start=\"3687\" data-end=\"3837\"\u003eFor laboratory, water, food, and environmental microbiology use only.\u003cbr data-start=\"3756\" data-end=\"3759\"\u003eNot for diagnostic or therapeutic applications without appropriate validation.\u003c\/p\u003e\n\u003chr data-start=\"3839\" data-end=\"3842\"\u003e\n\u003ch4 data-start=\"3844\" data-end=\"3869\"\u003eQuality \u0026amp; Compliance\u003c\/h4\u003e\n\u003cul data-start=\"3871\" data-end=\"4039\"\u003e\n\u003cli data-start=\"3871\" data-end=\"3918\"\u003e\n\u003cp data-start=\"3873\" data-end=\"3918\"\u003eSelective enrichment medium for enterococci\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3919\" data-end=\"3972\"\u003e\n\u003cp data-start=\"3921\" data-end=\"3972\"\u003eSuitable for ISO 7899-1 and DIN-aligned workflows\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3973\" data-end=\"4039\"\u003e\n\u003cp data-start=\"3975\" data-end=\"4039\"\u003eManufactured under controlled conditions for batch consistency\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"4041\" data-end=\"4044\"\u003e\n\u003ch4 data-start=\"4046\" data-end=\"4078\"\u003eCustoms \u0026amp; Trade Information\u003c\/h4\u003e\n\u003cp data-start=\"4080\" data-end=\"4185\"\u003eHS \/ AHECC Code: \u003cstrong data-start=\"4097\" data-end=\"4111\"\u003e3821.00.00\u003c\/strong\u003e\u003cbr data-start=\"4111\" data-end=\"4114\"\u003ePrepared culture media for development or maintenance of microorganisms\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"100G","offer_id":48052839252223,"sku":"AS-1128-DH-LS-100G","price":45.0,"currency_code":"AUD","in_stock":true},{"title":"500G","offer_id":48052839284991,"sku":"AS-1128-DH-LS-500G","price":115.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_f61d8bcc-cf05-4cfc-9e31-a645089afa1c.jpg?v=1771063025","url":"https:\/\/ausamics.com.au\/products\/azide-dextrose-broth-rothe-adb","provider":"AuSaMicS","version":"1.0","type":"link"}