{"product_id":"bile-esculin-agar","title":"Bile Esculin Agar","description":"\u003ch3 data-start=\"488\" data-end=\"509\"\u003eBile Esculin Agar\u003c\/h3\u003e\n\u003cp data-start=\"511\" data-end=\"588\"\u003eSelective \u0026amp; Differential Medium for Presumptive Identification of Enterococci\u003c\/p\u003e\n\u003cp data-start=\"590\" data-end=\"617\"\u003eCatalog Number: \u003cstrong data-start=\"606\" data-end=\"617\"\u003eAS-1140\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"619\" data-end=\"622\"\u003e\n\u003ch4 data-start=\"624\" data-end=\"637\"\u003eOverview\u003c\/h4\u003e\n\u003cp data-start=\"639\" data-end=\"947\"\u003eBile Esculin Agar is a \u003cstrong data-start=\"662\" data-end=\"713\"\u003eselective and differential solid culture medium\u003c\/strong\u003e widely used for the \u003cstrong data-start=\"734\" data-end=\"793\"\u003eisolation and presumptive identification of enterococci\u003c\/strong\u003e (\u003cem data-start=\"795\" data-end=\"856\"\u003eEnterococcus faecalis, E. faecium, E. durans, E. gallinarum\u003c\/em\u003e, etc.) and \u003cstrong data-start=\"868\" data-end=\"892\"\u003eGroup D streptococci\u003c\/strong\u003e from clinical, food, water, and environmental samples.\u003c\/p\u003e\n\u003cp data-start=\"949\" data-end=\"1296\"\u003eSelectivity is achieved through \u003cstrong data-start=\"981\" data-end=\"998\"\u003ebile (oxgall)\u003c\/strong\u003e, which inhibits most Gram-positive bacteria other than enterococci. Differential identification is based on \u003cstrong data-start=\"1107\" data-end=\"1129\"\u003eesculin hydrolysis\u003c\/strong\u003e; in the presence of \u003cstrong data-start=\"1150\" data-end=\"1168\"\u003eferric citrate\u003c\/strong\u003e, hydrolysed esculin forms a \u003cstrong data-start=\"1197\" data-end=\"1219\"\u003eblack iron complex\u003c\/strong\u003e, producing characteristic blackening of the medium around positive colonies.\u003c\/p\u003e\n\u003cp data-start=\"1298\" data-end=\"1460\"\u003eBile Esculin Agar is referenced in \u003cstrong data-start=\"1333\" data-end=\"1346\"\u003eCLSI M100\u003c\/strong\u003e, \u003cstrong data-start=\"1348\" data-end=\"1362\"\u003eISO 7899-2\u003c\/strong\u003e, and \u003cstrong data-start=\"1368\" data-end=\"1379\"\u003eFDA-BAM\u003c\/strong\u003e and remains one of the most reliable media for enterococcal screening worldwide.\u003c\/p\u003e\n\u003chr data-start=\"1462\" data-end=\"1465\"\u003e\n\u003ch4 data-start=\"1467\" data-end=\"1495\"\u003eKey Features \u0026amp; Benefits\u003c\/h4\u003e\n\u003cul data-start=\"1497\" data-end=\"1820\"\u003e\n\u003cli data-start=\"1497\" data-end=\"1551\"\u003e\n\u003cp data-start=\"1499\" data-end=\"1551\"\u003eSelective for enterococci and Group D streptococci\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1552\" data-end=\"1610\"\u003e\n\u003cp data-start=\"1554\" data-end=\"1610\"\u003eEsculin hydrolysis produces clear black halo reactions\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1611\" data-end=\"1666\"\u003e\n\u003cp data-start=\"1613\" data-end=\"1666\"\u003eRapid presumptive identification within 24–48 hours\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1667\" data-end=\"1746\"\u003e\n\u003cp data-start=\"1669\" data-end=\"1746\"\u003eDoes \u003cstrong data-start=\"1674\" data-end=\"1681\"\u003enot\u003c\/strong\u003e contain sodium azide, allowing improved growth of some strains\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1747\" data-end=\"1820\"\u003e\n\u003cp data-start=\"1749\" data-end=\"1820\"\u003eWidely referenced in clinical, food, and water microbiology standards\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"1822\" data-end=\"1825\"\u003e\n\u003ch4 data-start=\"1827\" data-end=\"1852\"\u003eTypical Applications\u003c\/h4\u003e\n\u003cp data-start=\"1854\" data-end=\"1877\"\u003eClinical Microbiology\u003c\/p\u003e\n\u003cul data-start=\"1878\" data-end=\"2032\"\u003e\n\u003cli data-start=\"1878\" data-end=\"1969\"\u003e\n\u003cp data-start=\"1880\" data-end=\"1969\"\u003ePresumptive identification of enterococci from urine, blood, wound, and other specimens\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1970\" data-end=\"2032\"\u003e\n\u003cp data-start=\"1972\" data-end=\"2032\"\u003eSupportive screening in antimicrobial resistance workflows\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2034\" data-end=\"2061\"\u003eFood \u0026amp; Water Microbiology\u003c\/p\u003e\n\u003cul data-start=\"2062\" data-end=\"2215\"\u003e\n\u003cli data-start=\"2062\" data-end=\"2144\"\u003e\n\u003cp data-start=\"2064\" data-end=\"2144\"\u003eIsolation of enterococci from foods, drinking water, and environmental samples\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2145\" data-end=\"2215\"\u003e\n\u003cp data-start=\"2147\" data-end=\"2215\"\u003eIndicator organism testing in hygiene and quality-control programs\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2217\" data-end=\"2252\"\u003eResearch \u0026amp; Reference Laboratories\u003c\/p\u003e\n\u003cul data-start=\"2253\" data-end=\"2357\"\u003e\n\u003cli data-start=\"2253\" data-end=\"2299\"\u003e\n\u003cp data-start=\"2255\" data-end=\"2299\"\u003eConfirmation testing and method validation\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2300\" data-end=\"2357\"\u003e\n\u003cp data-start=\"2302\" data-end=\"2357\"\u003eTeaching and training in selective\/differential media\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"2359\" data-end=\"2362\"\u003e\n\u003ch4 data-start=\"2364\" data-end=\"2392\"\u003ePrinciple of the Medium\u003c\/h4\u003e\n\u003cul data-start=\"2394\" data-end=\"2603\"\u003e\n\u003cli data-start=\"2394\" data-end=\"2471\"\u003e\n\u003cp data-start=\"2396\" data-end=\"2471\"\u003e\u003cstrong data-start=\"2396\" data-end=\"2413\"\u003eBile (oxgall)\u003c\/strong\u003e inhibits most Gram-positive bacteria except enterococci\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2472\" data-end=\"2529\"\u003e\n\u003cp data-start=\"2474\" data-end=\"2529\"\u003e\u003cstrong data-start=\"2474\" data-end=\"2485\"\u003eEsculin\u003c\/strong\u003e is hydrolysed by enterococci to esculetin\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2530\" data-end=\"2603\"\u003e\n\u003cp data-start=\"2532\" data-end=\"2603\"\u003e\u003cstrong data-start=\"2532\" data-end=\"2550\"\u003eFerric citrate\u003c\/strong\u003e reacts with esculetin to form a black iron complex\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2605\" data-end=\"2714\"\u003eA \u003cstrong data-start=\"2607\" data-end=\"2628\"\u003epositive reaction\u003c\/strong\u003e is indicated by \u003cstrong data-start=\"2645\" data-end=\"2697\"\u003eblackening of the medium or a black halo (≥5 mm)\u003c\/strong\u003e around colonies.\u003c\/p\u003e\n\u003chr data-start=\"2716\" data-end=\"2719\"\u003e\n\u003ch4 data-start=\"2721\" data-end=\"2759\"\u003eComposition (Typical – per litre)\u003c\/h4\u003e\n\u003cul data-start=\"2761\" data-end=\"2967\"\u003e\n\u003cli data-start=\"2761\" data-end=\"2791\"\u003e\n\u003cp data-start=\"2763\" data-end=\"2791\"\u003eTryptone \/ peptone: 17.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2792\" data-end=\"2822\"\u003e\n\u003cp data-start=\"2794\" data-end=\"2822\"\u003eMeat (beef) extract: 3.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2823\" data-end=\"2847\"\u003e\n\u003cp data-start=\"2825\" data-end=\"2847\"\u003eYeast extract: 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2848\" data-end=\"2878\"\u003e\n\u003cp data-start=\"2850\" data-end=\"2878\"\u003eOxgall (bile): 10.0–15.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2879\" data-end=\"2897\"\u003e\n\u003cp data-start=\"2881\" data-end=\"2897\"\u003eEsculin: 1.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2898\" data-end=\"2923\"\u003e\n\u003cp data-start=\"2900\" data-end=\"2923\"\u003eFerric citrate: 0.5 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2924\" data-end=\"2950\"\u003e\n\u003cp data-start=\"2926\" data-end=\"2950\"\u003eSodium chloride: 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2951\" data-end=\"2967\"\u003e\n\u003cp data-start=\"2953\" data-end=\"2967\"\u003eAgar: 15.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2969\" data-end=\"3001\"\u003eFinal pH at 25 °C: \u003cstrong data-start=\"2988\" data-end=\"3001\"\u003e7.1 ± 0.2\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"3003\" data-end=\"3006\"\u003e\n\u003ch4 data-start=\"3008\" data-end=\"3034\"\u003ePreparation (Typical)\u003c\/h4\u003e\n\u003cul data-start=\"3036\" data-end=\"3315\"\u003e\n\u003cli data-start=\"3036\" data-end=\"3110\"\u003e\n\u003cp data-start=\"3038\" data-end=\"3110\"\u003eSuspend \u003cstrong data-start=\"3046\" data-end=\"3061\"\u003e51.5–56.5 g\u003c\/strong\u003e of dehydrated medium in \u003cstrong data-start=\"3086\" data-end=\"3093\"\u003e1 L\u003c\/strong\u003e purified water\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3111\" data-end=\"3191\"\u003e\n\u003cp data-start=\"3113\" data-end=\"3191\"\u003eHeat with frequent agitation and \u003cstrong data-start=\"3146\" data-end=\"3167\"\u003eboil for 1 minute\u003c\/strong\u003e until fully dissolved\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3192\" data-end=\"3244\"\u003e\n\u003cp data-start=\"3194\" data-end=\"3244\"\u003e\u003cstrong data-start=\"3194\" data-end=\"3214\"\u003eDo not autoclave\u003c\/strong\u003e (esculin is heat-sensitive)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3245\" data-end=\"3315\"\u003e\n\u003cp data-start=\"3247\" data-end=\"3315\"\u003eCool to \u003cstrong data-start=\"3255\" data-end=\"3267\"\u003e45–50 °C\u003c\/strong\u003e, mix well, and pour into sterile Petri dishes\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3317\" data-end=\"3320\"\u003e\n\u003ch4 data-start=\"3322\" data-end=\"3337\"\u003eIncubation\u003c\/h4\u003e\n\u003cul data-start=\"3339\" data-end=\"3407\"\u003e\n\u003cli data-start=\"3339\" data-end=\"3371\"\u003e\n\u003cp data-start=\"3341\" data-end=\"3371\"\u003e\u003cstrong data-start=\"3341\" data-end=\"3369\"\u003e35–37 °C for 24–48 hours\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3372\" data-end=\"3407\"\u003e\n\u003cp data-start=\"3374\" data-end=\"3407\"\u003eExamine at both \u003cstrong data-start=\"3390\" data-end=\"3407\"\u003e24 h and 48 h\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3409\" data-end=\"3412\"\u003e\n\u003ch4 data-start=\"3414\" data-end=\"3433\"\u003eInterpretation\u003c\/h4\u003e\n\u003cul data-start=\"3435\" data-end=\"3618\"\u003e\n\u003cli data-start=\"3435\" data-end=\"3550\"\u003e\n\u003cp data-start=\"3437\" data-end=\"3550\"\u003e\u003cstrong data-start=\"3437\" data-end=\"3476\"\u003eEnterococci \/ Group D streptococci:\u003c\/strong\u003e\u003cbr data-start=\"3476\" data-end=\"3479\"\u003eGrowth with black or dark-brown colonies and surrounding black halo\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3551\" data-end=\"3618\"\u003e\n\u003cp data-start=\"3553\" data-end=\"3618\"\u003e\u003cstrong data-start=\"3553\" data-end=\"3573\"\u003eNon-enterococci:\u003c\/strong\u003e\u003cbr data-start=\"3573\" data-end=\"3576\"\u003eNo growth or growth without blackening\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3620\" data-end=\"3623\"\u003e\n\u003ch4 data-start=\"3625\" data-end=\"3649\"\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cp data-start=\"3651\" data-end=\"3670\"\u003eDehydrated medium\u003c\/p\u003e\n\u003cul data-start=\"3671\" data-end=\"3717\"\u003e\n\u003cli data-start=\"3671\" data-end=\"3717\"\u003e\n\u003cp data-start=\"3673\" data-end=\"3717\"\u003eStore below \u003cstrong data-start=\"3685\" data-end=\"3694\"\u003e30 °C\u003c\/strong\u003e in a cool, dry place\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"3719\" data-end=\"3736\"\u003ePrepared plates\u003c\/p\u003e\n\u003cul data-start=\"3737\" data-end=\"3807\"\u003e\n\u003cli data-start=\"3737\" data-end=\"3782\"\u003e\n\u003cp data-start=\"3739\" data-end=\"3782\"\u003eStore at \u003cstrong data-start=\"3748\" data-end=\"3758\"\u003e2–8 °C\u003c\/strong\u003e, protected from light\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3783\" data-end=\"3807\"\u003e\n\u003cp data-start=\"3785\" data-end=\"3807\"\u003eUse within \u003cstrong data-start=\"3796\" data-end=\"3807\"\u003e4 weeks\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3809\" data-end=\"3812\"\u003e\n\u003ch4 data-start=\"3814\" data-end=\"3831\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp data-start=\"3833\" data-end=\"3914\"\u003eFor \u003cstrong data-start=\"3837\" data-end=\"3913\"\u003eclinical, food, water, environmental, and research microbiology use only\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp data-start=\"3916\" data-end=\"3940\"\u003eNot for therapeutic use.\u003c\/p\u003e\n\u003chr data-start=\"3942\" data-end=\"3945\"\u003e\n\u003ch4 data-start=\"3947\" data-end=\"3972\"\u003eQuality \u0026amp; References\u003c\/h4\u003e\n\u003cul data-start=\"3974\" data-end=\"4254\"\u003e\n\u003cli data-start=\"3974\" data-end=\"4038\"\u003e\n\u003cp data-start=\"3976\" data-end=\"4038\"\u003eReferenced in \u003cstrong data-start=\"3990\" data-end=\"4003\"\u003eCLSI M100\u003c\/strong\u003e, \u003cstrong data-start=\"4005\" data-end=\"4019\"\u003eISO 7899-2\u003c\/strong\u003e, and \u003cstrong data-start=\"4025\" data-end=\"4036\"\u003eFDA-BAM\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4039\" data-end=\"4092\"\u003e\n\u003cp data-start=\"4041\" data-end=\"4092\"\u003eBased on methods described by \u003cstrong data-start=\"4071\" data-end=\"4090\"\u003eFacklam \u0026amp; Moody\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4093\" data-end=\"4159\"\u003e\n\u003cp data-start=\"4095\" data-end=\"4159\"\u003eManufactured under controlled conditions for batch consistency\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4160\" data-end=\"4203\"\u003e\n\u003cp data-start=\"4162\" data-end=\"4203\"\u003eCertificate of Analysis (COA) available\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4204\" data-end=\"4254\"\u003e\n\u003cp data-start=\"4206\" data-end=\"4254\"\u003eSafety Data Sheet (SDS) available upon request\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ausamics Life Science","offers":[{"title":"100G","offer_id":48104119697663,"sku":"AS-1140-100G","price":55.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_70702848-37d4-4ef0-8948-1a3e41ca2964.jpg?v=1771063018","url":"https:\/\/ausamics.com.au\/products\/bile-esculin-agar","provider":"AuSaMicS","version":"1.0","type":"link"}