{"product_id":"bile-esculin-azide-agar","title":"Bile Esculin Azide Agar","description":"\u003ch3 data-start=\"506\" data-end=\"533\"\u003eBile Esculin Azide Agar\u003c\/h3\u003e\n\u003cp data-start=\"535\" data-end=\"602\"\u003eHighly Selective \u0026amp; Differential Medium for Isolation of Enterococci\u003c\/p\u003e\n\u003cp data-start=\"604\" data-end=\"631\"\u003eCatalog Number: \u003cstrong data-start=\"620\" data-end=\"631\"\u003eAS-1141\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"633\" data-end=\"636\"\u003e\n\u003ch4 data-start=\"638\" data-end=\"651\"\u003eOverview\u003c\/h4\u003e\n\u003cp data-start=\"653\" data-end=\"960\"\u003eBile Esculin Azide Agar is a \u003cstrong data-start=\"682\" data-end=\"740\"\u003ehighly selective and differential solid culture medium\u003c\/strong\u003e specifically designed for the \u003cstrong data-start=\"771\" data-end=\"830\"\u003eisolation and presumptive identification of enterococci\u003c\/strong\u003e, including \u003cem data-start=\"842\" data-end=\"889\"\u003eEnterococcus faecalis, E. faecium, E. durans,\u003c\/em\u003e and \u003cem data-start=\"894\" data-end=\"904\"\u003eE. avium\u003c\/em\u003e, from clinical, food, water, and environmental samples.\u003c\/p\u003e\n\u003cp data-start=\"962\" data-end=\"1291\"\u003eThe medium combines \u003cstrong data-start=\"982\" data-end=\"1008\"\u003ethree selective agents\u003c\/strong\u003e—bile (oxgall), \u003cstrong data-start=\"1024\" data-end=\"1040\"\u003esodium azide\u003c\/strong\u003e, and salt—with the \u003cstrong data-start=\"1060\" data-end=\"1102\"\u003eesculin–ferric citrate reaction system\u003c\/strong\u003e. This formulation provides \u003cstrong data-start=\"1130\" data-end=\"1153\"\u003emaximum selectivity\u003c\/strong\u003e, allowing enterococci to grow while effectively inhibiting streptococci, staphylococci, Gram-negative bacteria, and most competing flora.\u003c\/p\u003e\n\u003cp data-start=\"1293\" data-end=\"1506\"\u003eBile Esculin Azide Agar is the \u003cstrong data-start=\"1324\" data-end=\"1367\"\u003eofficial reference medium in ISO 7899-2\u003c\/strong\u003e for the detection and enumeration of intestinal enterococci in water and is widely cited in food microbiology and public-health standards.\u003c\/p\u003e\n\u003chr data-start=\"1508\" data-end=\"1511\"\u003e\n\u003ch4 data-start=\"1513\" data-end=\"1541\"\u003eKey Features \u0026amp; Benefits\u003c\/h4\u003e\n\u003cul data-start=\"1543\" data-end=\"1902\"\u003e\n\u003cli data-start=\"1543\" data-end=\"1614\"\u003e\n\u003cp data-start=\"1545\" data-end=\"1614\"\u003eTriple selective system for maximum suppression of background flora\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1615\" data-end=\"1698\"\u003e\n\u003cp data-start=\"1617\" data-end=\"1698\"\u003eStrong inhibition of Gram-negative bacteria and non-enterococcal Gram-positives\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1699\" data-end=\"1775\"\u003e\n\u003cp data-start=\"1701\" data-end=\"1775\"\u003eEsculin hydrolysis produces intense black halos around positive colonies\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1776\" data-end=\"1835\"\u003e\n\u003cp data-start=\"1778\" data-end=\"1835\"\u003eFaster and cleaner results than Bile Esculin Agar alone\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1836\" data-end=\"1902\"\u003e\n\u003cp data-start=\"1838\" data-end=\"1902\"\u003eClear presumptive results typically visible within 18–24 hours\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"1904\" data-end=\"1907\"\u003e\n\u003ch4 data-start=\"1909\" data-end=\"1934\"\u003eTypical Applications\u003c\/h4\u003e\n\u003cp data-start=\"1936\" data-end=\"1972\"\u003eWater \u0026amp; Environmental Microbiology\u003c\/p\u003e\n\u003cul data-start=\"1973\" data-end=\"2091\"\u003e\n\u003cli data-start=\"1973\" data-end=\"2040\"\u003e\n\u003cp data-start=\"1975\" data-end=\"2040\"\u003eEnumeration of enterococci in drinking, bathing, and wastewater\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2041\" data-end=\"2091\"\u003e\n\u003cp data-start=\"2043\" data-end=\"2091\"\u003eReference method for \u003cstrong data-start=\"2064\" data-end=\"2078\"\u003eISO 7899-2\u003c\/strong\u003e compliance\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2093\" data-end=\"2112\"\u003eFood Microbiology\u003c\/p\u003e\n\u003cul data-start=\"2113\" data-end=\"2235\"\u003e\n\u003cli data-start=\"2113\" data-end=\"2184\"\u003e\n\u003cp data-start=\"2115\" data-end=\"2184\"\u003eDetection of faecal enterococci in dairy, meat, and processed foods\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2185\" data-end=\"2235\"\u003e\n\u003cp data-start=\"2187\" data-end=\"2235\"\u003eHygiene monitoring and quality-control testing\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2237\" data-end=\"2271\"\u003eClinical \u0026amp; Research Laboratories\u003c\/p\u003e\n\u003cul data-start=\"2272\" data-end=\"2390\"\u003e\n\u003cli data-start=\"2272\" data-end=\"2327\"\u003e\n\u003cp data-start=\"2274\" data-end=\"2327\"\u003eConfirmation of enterococci from clinical specimens\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2328\" data-end=\"2390\"\u003e\n\u003cp data-start=\"2330\" data-end=\"2390\"\u003eSupportive screening in antimicrobial-resistance workflows\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"2392\" data-end=\"2395\"\u003e\n\u003ch4 data-start=\"2397\" data-end=\"2425\"\u003ePrinciple of the Medium\u003c\/h4\u003e\n\u003cul data-start=\"2427\" data-end=\"2725\"\u003e\n\u003cli data-start=\"2427\" data-end=\"2485\"\u003e\n\u003cp data-start=\"2429\" data-end=\"2485\"\u003e\u003cstrong data-start=\"2429\" data-end=\"2446\"\u003eBile (oxgall)\u003c\/strong\u003e inhibits many Gram-positive bacteria\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2486\" data-end=\"2584\"\u003e\n\u003cp data-start=\"2488\" data-end=\"2584\"\u003e\u003cstrong data-start=\"2488\" data-end=\"2504\"\u003eSodium azide\u003c\/strong\u003e suppresses cytochrome-containing organisms, especially Gram-negative bacteria\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2585\" data-end=\"2642\"\u003e\n\u003cp data-start=\"2587\" data-end=\"2642\"\u003e\u003cstrong data-start=\"2587\" data-end=\"2598\"\u003eEsculin\u003c\/strong\u003e is hydrolysed by enterococci to esculetin\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2643\" data-end=\"2725\"\u003e\n\u003cp data-start=\"2645\" data-end=\"2725\"\u003e\u003cstrong data-start=\"2645\" data-end=\"2672\"\u003eFerric ammonium citrate\u003c\/strong\u003e reacts with esculetin to form a black iron complex\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2727\" data-end=\"2835\"\u003eA \u003cstrong data-start=\"2729\" data-end=\"2750\"\u003epositive reaction\u003c\/strong\u003e is indicated by \u003cstrong data-start=\"2767\" data-end=\"2834\"\u003eblack or dark-brown colonies surrounded by a black halo (≥5 mm)\u003c\/strong\u003e.\u003c\/p\u003e\n\u003chr data-start=\"2837\" data-end=\"2840\"\u003e\n\u003ch4 data-start=\"2842\" data-end=\"2880\"\u003eComposition (Typical – per litre)\u003c\/h4\u003e\n\u003cul data-start=\"2882\" data-end=\"3099\"\u003e\n\u003cli data-start=\"2882\" data-end=\"2901\"\u003e\n\u003cp data-start=\"2884\" data-end=\"2901\"\u003ePeptone: 17.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2902\" data-end=\"2925\"\u003e\n\u003cp data-start=\"2904\" data-end=\"2925\"\u003eBeef extract: 3.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2926\" data-end=\"2950\"\u003e\n\u003cp data-start=\"2928\" data-end=\"2950\"\u003eYeast extract: 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2951\" data-end=\"2976\"\u003e\n\u003cp data-start=\"2953\" data-end=\"2976\"\u003eOxgall (bile): 10.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2977\" data-end=\"2995\"\u003e\n\u003cp data-start=\"2979\" data-end=\"2995\"\u003eEsculin: 1.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2996\" data-end=\"3030\"\u003e\n\u003cp data-start=\"2998\" data-end=\"3030\"\u003eFerric ammonium citrate: 0.5 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3031\" data-end=\"3057\"\u003e\n\u003cp data-start=\"3033\" data-end=\"3057\"\u003eSodium chloride: 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3058\" data-end=\"3082\"\u003e\n\u003cp data-start=\"3060\" data-end=\"3082\"\u003eSodium azide: 0.15 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3083\" data-end=\"3099\"\u003e\n\u003cp data-start=\"3085\" data-end=\"3099\"\u003eAgar: 15.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"3101\" data-end=\"3133\"\u003eFinal pH at 25 °C: \u003cstrong data-start=\"3120\" data-end=\"3133\"\u003e7.1 ± 0.2\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"3135\" data-end=\"3138\"\u003e\n\u003ch4 data-start=\"3140\" data-end=\"3166\"\u003ePreparation (Typical)\u003c\/h4\u003e\n\u003cul data-start=\"3168\" data-end=\"3458\"\u003e\n\u003cli data-start=\"3168\" data-end=\"3238\"\u003e\n\u003cp data-start=\"3170\" data-end=\"3238\"\u003eSuspend \u003cstrong data-start=\"3178\" data-end=\"3189\"\u003e56.65 g\u003c\/strong\u003e of dehydrated medium in \u003cstrong data-start=\"3214\" data-end=\"3221\"\u003e1 L\u003c\/strong\u003e purified water\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3239\" data-end=\"3319\"\u003e\n\u003cp data-start=\"3241\" data-end=\"3319\"\u003eHeat with frequent agitation and \u003cstrong data-start=\"3274\" data-end=\"3295\"\u003eboil for 1 minute\u003c\/strong\u003e until fully dissolved\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3320\" data-end=\"3383\"\u003e\n\u003cp data-start=\"3322\" data-end=\"3383\"\u003e\u003cstrong data-start=\"3322\" data-end=\"3342\"\u003eDo not autoclave\u003c\/strong\u003e (esculin and azide are heat-sensitive)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3384\" data-end=\"3458\"\u003e\n\u003cp data-start=\"3386\" data-end=\"3458\"\u003eCool to \u003cstrong data-start=\"3394\" data-end=\"3406\"\u003e45–50 °C\u003c\/strong\u003e, swirl gently, and pour into sterile Petri dishes\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3460\" data-end=\"3463\"\u003e\n\u003ch4 data-start=\"3465\" data-end=\"3480\"\u003eIncubation\u003c\/h4\u003e\n\u003cul data-start=\"3482\" data-end=\"3577\"\u003e\n\u003cli data-start=\"3482\" data-end=\"3514\"\u003e\n\u003cp data-start=\"3484\" data-end=\"3514\"\u003e\u003cstrong data-start=\"3484\" data-end=\"3512\"\u003e35–37 °C for 24–48 hours\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3515\" data-end=\"3577\"\u003e\n\u003cp data-start=\"3517\" data-end=\"3577\"\u003eMost enterococci show positive reactions within \u003cstrong data-start=\"3565\" data-end=\"3577\"\u003e24 hours\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3579\" data-end=\"3582\"\u003e\n\u003ch4 data-start=\"3584\" data-end=\"3603\"\u003eInterpretation\u003c\/h4\u003e\n\u003cul data-start=\"3605\" data-end=\"3756\"\u003e\n\u003cli data-start=\"3605\" data-end=\"3688\"\u003e\n\u003cp data-start=\"3607\" data-end=\"3688\"\u003e\u003cstrong data-start=\"3607\" data-end=\"3623\"\u003eEnterococci:\u003c\/strong\u003e\u003cbr data-start=\"3623\" data-end=\"3626\"\u003eBlack or dark-brown colonies with a surrounding black halo\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3689\" data-end=\"3756\"\u003e\n\u003cp data-start=\"3691\" data-end=\"3756\"\u003e\u003cstrong data-start=\"3691\" data-end=\"3711\"\u003eOther organisms:\u003c\/strong\u003e\u003cbr data-start=\"3711\" data-end=\"3714\"\u003eNo growth or growth without blackening\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3758\" data-end=\"3761\"\u003e\n\u003ch4 data-start=\"3763\" data-end=\"3787\"\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cp data-start=\"3789\" data-end=\"3808\"\u003eDehydrated medium\u003c\/p\u003e\n\u003cul data-start=\"3809\" data-end=\"3855\"\u003e\n\u003cli data-start=\"3809\" data-end=\"3855\"\u003e\n\u003cp data-start=\"3811\" data-end=\"3855\"\u003eStore below \u003cstrong data-start=\"3823\" data-end=\"3832\"\u003e30 °C\u003c\/strong\u003e in a cool, dry place\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"3857\" data-end=\"3874\"\u003ePrepared plates\u003c\/p\u003e\n\u003cul data-start=\"3875\" data-end=\"3945\"\u003e\n\u003cli data-start=\"3875\" data-end=\"3920\"\u003e\n\u003cp data-start=\"3877\" data-end=\"3920\"\u003eStore at \u003cstrong data-start=\"3886\" data-end=\"3896\"\u003e2–8 °C\u003c\/strong\u003e, protected from light\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3921\" data-end=\"3945\"\u003e\n\u003cp data-start=\"3923\" data-end=\"3945\"\u003eUse within \u003cstrong data-start=\"3934\" data-end=\"3945\"\u003e3 weeks\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3947\" data-end=\"3950\"\u003e\n\u003ch4 data-start=\"3952\" data-end=\"3970\"\u003eSafety Notice\u003c\/h4\u003e\n\u003cp data-start=\"3972\" data-end=\"4183\"\u003eThis medium contains \u003cstrong data-start=\"3993\" data-end=\"4009\"\u003esodium azide\u003c\/strong\u003e, which is \u003cstrong data-start=\"4020\" data-end=\"4029\"\u003etoxic\u003c\/strong\u003e and may form explosive compounds with heavy metals.\u003cbr data-start=\"4081\" data-end=\"4084\"\u003eHandle with appropriate laboratory precautions and dispose of waste according to local regulations.\u003c\/p\u003e\n\u003chr data-start=\"4185\" data-end=\"4188\"\u003e\n\u003ch4 data-start=\"4190\" data-end=\"4207\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp data-start=\"4209\" data-end=\"4301\"\u003eFor \u003cstrong data-start=\"4213\" data-end=\"4300\"\u003elaboratory clinical, food, water, environmental, and research microbiology use only\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp data-start=\"4303\" data-end=\"4327\"\u003eNot for therapeutic use.\u003c\/p\u003e\n\u003chr data-start=\"4329\" data-end=\"4332\"\u003e\n\u003ch4 data-start=\"4334\" data-end=\"4359\"\u003eQuality \u0026amp; Compliance\u003c\/h4\u003e\n\u003cul data-start=\"4361\" data-end=\"4616\"\u003e\n\u003cli data-start=\"4361\" data-end=\"4408\"\u003e\n\u003cp data-start=\"4363\" data-end=\"4408\"\u003eOfficial reference medium in \u003cstrong data-start=\"4392\" data-end=\"4406\"\u003eISO 7899-2\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4409\" data-end=\"4454\"\u003e\n\u003cp data-start=\"4411\" data-end=\"4454\"\u003eCited in \u003cstrong data-start=\"4420\" data-end=\"4428\"\u003eAPHA\u003c\/strong\u003e and \u003cstrong data-start=\"4433\" data-end=\"4444\"\u003eFDA-BAM\u003c\/strong\u003e methods\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4455\" data-end=\"4521\"\u003e\n\u003cp data-start=\"4457\" data-end=\"4521\"\u003eManufactured under controlled conditions for batch consistency\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4522\" data-end=\"4565\"\u003e\n\u003cp data-start=\"4524\" data-end=\"4565\"\u003eCertificate of Analysis (COA) available\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4566\" data-end=\"4616\"\u003e\n\u003cp data-start=\"4568\" data-end=\"4616\"\u003eSafety Data Sheet (SDS) available upon request\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ausamics Life Science","offers":[{"title":"100G","offer_id":48104120582399,"sku":"AS-1141-100G","price":65.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_dc7d3f74-4b4c-4063-ba80-f17e3392407b.jpg?v=1771063018","url":"https:\/\/ausamics.com.au\/products\/bile-esculin-azide-agar","provider":"AuSaMicS","version":"1.0","type":"link"}