{"product_id":"bolton-selective-enrichment-broth-base","title":"Bolton Selective Enrichment Broth","description":"\u003ch3 data-start=\"42\" data-end=\"79\"\u003eBolton Selective Enrichment Broth\u003c\/h3\u003e\n\u003cp data-start=\"81\" data-end=\"178\"\u003eSelective Enrichment Medium for Thermotolerant \u003cem data-start=\"128\" data-end=\"143\"\u003eCampylobacter\u003c\/em\u003e spp.\u003cbr data-start=\"148\" data-end=\"151\"\u003eCatalog Number: \u003cstrong data-start=\"167\" data-end=\"178\"\u003eAS-1146\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"180\" data-end=\"183\"\u003e\n\u003ch4 data-start=\"185\" data-end=\"198\"\u003eOverview\u003c\/h4\u003e\n\u003cp data-start=\"200\" data-end=\"496\"\u003eBolton Selective Enrichment Broth (CAT: AS-1146) is the \u003cstrong data-start=\"256\" data-end=\"303\"\u003einternationally recognised reference medium\u003c\/strong\u003e for the selective enrichment of thermotolerant \u003cem data-start=\"351\" data-end=\"366\"\u003eCampylobacter\u003c\/em\u003e species, including \u003cem data-start=\"386\" data-end=\"397\"\u003eC. jejuni\u003c\/em\u003e, \u003cem data-start=\"399\" data-end=\"408\"\u003eC. coli\u003c\/em\u003e, \u003cem data-start=\"410\" data-end=\"419\"\u003eC. lari\u003c\/em\u003e, and \u003cem data-start=\"425\" data-end=\"441\"\u003eC. upsaliensis\u003c\/em\u003e, from foods, animal faeces, and environmental samples.\u003c\/p\u003e\n\u003cp data-start=\"498\" data-end=\"875\"\u003eThis medium is the \u003cstrong data-start=\"517\" data-end=\"547\"\u003emandatory enrichment broth\u003c\/strong\u003e specified in \u003cstrong data-start=\"561\" data-end=\"581\"\u003eISO 10272-1:2017\u003c\/strong\u003e, \u003cstrong data-start=\"583\" data-end=\"604\"\u003eFDA-BAM Chapter 7\u003c\/strong\u003e, and \u003cstrong data-start=\"610\" data-end=\"623\"\u003eUSDA-FSIS\u003c\/strong\u003e methods. Its formulation combines a rich nutrient base, haematinised (lysed) blood, and a four-antibiotic selective supplement to ensure excellent recovery of stressed or low-level \u003cem data-start=\"805\" data-end=\"820\"\u003eCampylobacter\u003c\/em\u003e while strongly suppressing competing background flora.\u003c\/p\u003e\n\u003chr data-start=\"877\" data-end=\"880\"\u003e\n\u003ch4 data-start=\"882\" data-end=\"910\"\u003eKey Features \u0026amp; Benefits\u003c\/h4\u003e\n\u003cul data-start=\"912\" data-end=\"1282\"\u003e\n\u003cli data-start=\"912\" data-end=\"975\"\u003e\n\u003cp data-start=\"914\" data-end=\"975\"\u003eOfficial reference enrichment broth in \u003cstrong data-start=\"953\" data-end=\"973\"\u003eISO 10272-1:2017\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"976\" data-end=\"1031\"\u003e\n\u003cp data-start=\"978\" data-end=\"1031\"\u003eOptimised for \u003cstrong data-start=\"992\" data-end=\"1024\"\u003ethermotolerant Campylobacter\u003c\/strong\u003e spp.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1032\" data-end=\"1104\"\u003e\n\u003cp data-start=\"1034\" data-end=\"1104\"\u003eHaematinised blood + pyruvate support resuscitation of injured cells\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1105\" data-end=\"1160\"\u003e\n\u003cp data-start=\"1107\" data-end=\"1160\"\u003eFour-antibiotic supplement ensures high selectivity\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1161\" data-end=\"1216\"\u003e\n\u003cp data-start=\"1163\" data-end=\"1216\"\u003eDesigned for \u003cstrong data-start=\"1176\" data-end=\"1214\"\u003emicroaerobic incubation at 41.5 °C\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1217\" data-end=\"1282\"\u003e\n\u003cp data-start=\"1219\" data-end=\"1282\"\u003eExcellent performance with heavily contaminated food matrices\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"1284\" data-end=\"1287\"\u003e\n\u003ch4 data-start=\"1289\" data-end=\"1314\"\u003eTypical Applications\u003c\/h4\u003e\n\u003cp data-start=\"1316\" data-end=\"1335\"\u003eFood Microbiology\u003c\/p\u003e\n\u003cul data-start=\"1336\" data-end=\"1471\"\u003e\n\u003cli data-start=\"1336\" data-end=\"1415\"\u003e\n\u003cp data-start=\"1338\" data-end=\"1415\"\u003eDetection of \u003cem data-start=\"1351\" data-end=\"1366\"\u003eCampylobacter\u003c\/em\u003e in poultry, meat, milk, and ready-to-eat foods\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1416\" data-end=\"1471\"\u003e\n\u003cp data-start=\"1418\" data-end=\"1471\"\u003eRegulatory and quality-control testing laboratories\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"1473\" data-end=\"1509\"\u003eClinical \u0026amp; Veterinary Microbiology\u003c\/p\u003e\n\u003cul data-start=\"1510\" data-end=\"1586\"\u003e\n\u003cli data-start=\"1510\" data-end=\"1586\"\u003e\n\u003cp data-start=\"1512\" data-end=\"1586\"\u003eEnrichment from faeces and clinical specimens with high background flora\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"1588\" data-end=\"1611\"\u003eEnvironmental Testing\u003c\/p\u003e\n\u003cul data-start=\"1612\" data-end=\"1680\"\u003e\n\u003cli data-start=\"1612\" data-end=\"1680\"\u003e\n\u003cp data-start=\"1614\" data-end=\"1680\"\u003eRecovery of \u003cem data-start=\"1626\" data-end=\"1641\"\u003eCampylobacter\u003c\/em\u003e from water and environmental samples\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"1682\" data-end=\"1701\"\u003eReference Methods\u003c\/p\u003e\n\u003cul data-start=\"1702\" data-end=\"1789\"\u003e\n\u003cli data-start=\"1702\" data-end=\"1729\"\u003e\n\u003cp data-start=\"1704\" data-end=\"1729\"\u003eISO 10272-1 (all parts)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1730\" data-end=\"1751\"\u003e\n\u003cp data-start=\"1732\" data-end=\"1751\"\u003eFDA-BAM Chapter 7\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"1752\" data-end=\"1789\"\u003e\n\u003cp data-start=\"1754\" data-end=\"1789\"\u003eUSDA-FSIS Campylobacter protocols\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"1791\" data-end=\"1794\"\u003e\n\u003ch4 data-start=\"1796\" data-end=\"1824\"\u003ePrinciple of the Medium\u003c\/h4\u003e\n\u003cp data-start=\"1826\" data-end=\"2293\"\u003eBolton Broth provides a \u003cstrong data-start=\"1850\" data-end=\"1897\"\u003ehighly nutritive and protective environment\u003c\/strong\u003e that supports the recovery of stressed \u003cem data-start=\"1937\" data-end=\"1952\"\u003eCampylobacter\u003c\/em\u003e cells. Sodium pyruvate, α-ketoglutarate, and haematinised blood neutralise toxic oxygen radicals, while the selective antibiotic combination suppresses Gram-positive bacteria, Enterobacteriaceae, yeasts, and moulds. Incubation under microaerobic conditions at elevated temperature selectively favours thermotolerant \u003cem data-start=\"2269\" data-end=\"2284\"\u003eCampylobacter\u003c\/em\u003e species.\u003c\/p\u003e\n\u003chr data-start=\"2295\" data-end=\"2298\"\u003e\n\u003ch4 data-start=\"2300\" data-end=\"2340\"\u003eComposition (per litre – base only)\u003c\/h4\u003e\n\u003cul data-start=\"2342\" data-end=\"2587\"\u003e\n\u003cli data-start=\"2342\" data-end=\"2366\"\u003e\n\u003cp data-start=\"2344\" data-end=\"2366\"\u003eMeat Peptone: 10.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2367\" data-end=\"2401\"\u003e\n\u003cp data-start=\"2369\" data-end=\"2401\"\u003eLactalbumin Hydrolysate: 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2402\" data-end=\"2426\"\u003e\n\u003cp data-start=\"2404\" data-end=\"2426\"\u003eYeast Extract: 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2427\" data-end=\"2453\"\u003e\n\u003cp data-start=\"2429\" data-end=\"2453\"\u003eSodium Chloride: 5.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2454\" data-end=\"2480\"\u003e\n\u003cp data-start=\"2456\" data-end=\"2480\"\u003eSodium Pyruvate: 0.5 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2481\" data-end=\"2512\"\u003e\n\u003cp data-start=\"2483\" data-end=\"2512\"\u003eSodium Metabisulfite: 0.5 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2513\" data-end=\"2543\"\u003e\n\u003cp data-start=\"2515\" data-end=\"2543\"\u003eα-Ketoglutaric Acid: 1.0 g\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2544\" data-end=\"2587\"\u003e\n\u003cp data-start=\"2546\" data-end=\"2587\"\u003eHaematinised (lysed) horse blood: 50 mL\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2589\" data-end=\"2621\"\u003eFinal pH: \u003cstrong data-start=\"2599\" data-end=\"2621\"\u003e7.4 ± 0.2 at 25 °C\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"2623\" data-end=\"2626\"\u003e\n\u003ch4 data-start=\"2628\" data-end=\"2684\"\u003eAntibiotic Supplement (Bolton Selective Supplement)\u003c\/h4\u003e\n\u003cp data-start=\"2686\" data-end=\"2724\"\u003eAdded after sterilisation and cooling:\u003c\/p\u003e\n\u003cul data-start=\"2726\" data-end=\"2840\"\u003e\n\u003cli data-start=\"2726\" data-end=\"2747\"\u003e\n\u003cp data-start=\"2728\" data-end=\"2747\"\u003eVancomycin: 20 mg\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2748\" data-end=\"2771\"\u003e\n\u003cp data-start=\"2750\" data-end=\"2771\"\u003eTrimethoprim: 20 mg\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2772\" data-end=\"2795\"\u003e\n\u003cp data-start=\"2774\" data-end=\"2795\"\u003eCefoperazone: 20 mg\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"2796\" data-end=\"2840\"\u003e\n\u003cp data-start=\"2798\" data-end=\"2840\"\u003eAmphotericin B (or Cycloheximide): 10 mg\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2842\" data-end=\"2908\"\u003e(One vial per 500 mL or 1 L, depending on supplier specification.)\u003c\/p\u003e\n\u003chr data-start=\"2910\" data-end=\"2913\"\u003e\n\u003ch4 data-start=\"2915\" data-end=\"2931\"\u003ePreparation\u003c\/h4\u003e\n\u003col data-start=\"2933\" data-end=\"3243\"\u003e\n\u003cli data-start=\"2933\" data-end=\"2999\"\u003e\n\u003cp data-start=\"2936\" data-end=\"2999\"\u003eSuspend \u003cstrong data-start=\"2944\" data-end=\"2954\"\u003e27.0 g\u003c\/strong\u003e Bolton Broth Base in 950 mL purified water\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3000\" data-end=\"3043\"\u003e\n\u003cp data-start=\"3003\" data-end=\"3043\"\u003eAutoclave at \u003cstrong data-start=\"3016\" data-end=\"3041\"\u003e121 °C for 15 minutes\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3044\" data-end=\"3069\"\u003e\n\u003cp data-start=\"3047\" data-end=\"3069\"\u003eCool to \u003cstrong data-start=\"3055\" data-end=\"3067\"\u003e45–50 °C\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3070\" data-end=\"3114\"\u003e\n\u003cp data-start=\"3073\" data-end=\"3114\"\u003eAdd \u003cstrong data-start=\"3077\" data-end=\"3112\"\u003e50 mL sterile lysed horse blood\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3115\" data-end=\"3177\"\u003e\n\u003cp data-start=\"3118\" data-end=\"3177\"\u003eAseptically add reconstituted Bolton Selective Supplement\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3178\" data-end=\"3243\"\u003e\n\u003cp data-start=\"3181\" data-end=\"3243\"\u003eMix gently and dispense \u003cstrong data-start=\"3205\" data-end=\"3217\"\u003e10–25 mL\u003c\/strong\u003e into sterile containers\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003chr data-start=\"3245\" data-end=\"3248\"\u003e\n\u003ch4 data-start=\"3250\" data-end=\"3265\"\u003eIncubation\u003c\/h4\u003e\n\u003cul data-start=\"3267\" data-end=\"3421\"\u003e\n\u003cli data-start=\"3267\" data-end=\"3311\"\u003e\n\u003cp data-start=\"3269\" data-end=\"3311\"\u003e\u003cstrong data-start=\"3269\" data-end=\"3292\"\u003e37 °C for 4–6 hours\u003c\/strong\u003e (pre-enrichment)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3312\" data-end=\"3362\"\u003e\n\u003cp data-start=\"3314\" data-end=\"3362\"\u003eThen \u003cstrong data-start=\"3319\" data-end=\"3330\"\u003e41.5 °C\u003c\/strong\u003e to a total of \u003cstrong data-start=\"3345\" data-end=\"3360\"\u003e40–44 hours\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3363\" data-end=\"3421\"\u003e\n\u003cp data-start=\"3365\" data-end=\"3421\"\u003eAtmosphere: \u003cstrong data-start=\"3377\" data-end=\"3393\"\u003eMicroaerobic\u003c\/strong\u003e (≈5% O₂, 10% CO₂, 85% N₂)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3423\" data-end=\"3426\"\u003e\n\u003ch4 data-start=\"3428\" data-end=\"3443\"\u003eSubculture\u003c\/h4\u003e\n\u003cp data-start=\"3445\" data-end=\"3508\"\u003eAfter \u003cstrong data-start=\"3451\" data-end=\"3467\"\u003e44 ± 4 hours\u003c\/strong\u003e, subculture onto selective agar such as:\u003c\/p\u003e\n\u003cul data-start=\"3510\" data-end=\"3536\"\u003e\n\u003cli data-start=\"3510\" data-end=\"3519\"\u003e\n\u003cp data-start=\"3512\" data-end=\"3519\"\u003emCCDA\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3520\" data-end=\"3536\"\u003e\n\u003cp data-start=\"3522\" data-end=\"3536\"\u003eKarmali Agar\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3538\" data-end=\"3541\"\u003e\n\u003ch4 data-start=\"3543\" data-end=\"3567\"\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cul data-start=\"3569\" data-end=\"3719\"\u003e\n\u003cli data-start=\"3569\" data-end=\"3641\"\u003e\n\u003cp data-start=\"3571\" data-end=\"3641\"\u003eDehydrated base: Store below \u003cstrong data-start=\"3600\" data-end=\"3609\"\u003e30 °C\u003c\/strong\u003e, dry and protected from light\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3642\" data-end=\"3719\"\u003e\n\u003cp data-start=\"3644\" data-end=\"3719\"\u003ePrepared broth (with blood): Store at \u003cstrong data-start=\"3682\" data-end=\"3692\"\u003e2–8 °C\u003c\/strong\u003e, use within \u003cstrong data-start=\"3705\" data-end=\"3717\"\u003e24 hours\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr data-start=\"3721\" data-end=\"3724\"\u003e\n\u003ch4 data-start=\"3726\" data-end=\"3743\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp data-start=\"3745\" data-end=\"3884\"\u003eFor \u003cstrong data-start=\"3749\" data-end=\"3831\"\u003elaboratory food, clinical, veterinary, and environmental microbiology use only\u003c\/strong\u003e.\u003cbr data-start=\"3832\" data-end=\"3835\"\u003eNot intended for human or animal therapeutic use.\u003c\/p\u003e\n\u003chr data-start=\"3886\" data-end=\"3889\"\u003e\n\u003ch4 data-start=\"3891\" data-end=\"3916\"\u003eQuality \u0026amp; Compliance\u003c\/h4\u003e\n\u003cul data-start=\"3918\" data-end=\"4067\"\u003e\n\u003cli data-start=\"3918\" data-end=\"3967\"\u003e\n\u003cp data-start=\"3920\" data-end=\"3967\"\u003eConforms to \u003cstrong data-start=\"3932\" data-end=\"3952\"\u003eISO 10272-1:2017\u003c\/strong\u003e requirements\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3968\" data-end=\"4022\"\u003e\n\u003cp data-start=\"3970\" data-end=\"4022\"\u003eSuitable for regulated and accredited laboratories\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"4023\" data-end=\"4067\"\u003e\n\u003cp data-start=\"4025\" data-end=\"4067\"\u003eCOA, SDS, and TDS available upon request\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Ausamics Life Science","offers":[{"title":"100G","offer_id":48104311718143,"sku":"AS-1146-100G","price":57.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_82b95f0b-7b74-41f9-92f8-fbc93afe8bc9.jpg?v=1771063015","url":"https:\/\/ausamics.com.au\/products\/bolton-selective-enrichment-broth-base","provider":"AuSaMicS","version":"1.0","type":"link"}