{"product_id":"bushnell-haas-agar","title":"Bushnell-Haas Agar | Hydrocarbon Degrading Bacteria Medium | Ausamics","description":"\u003ch2 data-start=\"358\" data-end=\"380\"\u003eBushnell-Haas Agar\u003c\/h2\u003e\n\u003cp data-start=\"381\" data-end=\"537\"\u003e\u003cstrong data-start=\"381\" data-end=\"446\"\u003eMineral Salts Medium for Hydrocarbon-Degrading Microorganisms\u003c\/strong\u003e\u003cbr data-start=\"446\" data-end=\"449\"\u003e\u003cstrong data-start=\"449\" data-end=\"470\"\u003eCat. No.: AS-1161\u003c\/strong\u003e\u003cbr data-start=\"470\" data-end=\"473\"\u003e\u003cstrong data-start=\"473\" data-end=\"537\"\u003eHS Code: 3821.00 – Prepared Culture Media for Microorganisms\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"539\" data-end=\"542\"\u003e\n\u003ch3 data-start=\"544\" data-end=\"554\"\u003eOverview\u003c\/h3\u003e\n\u003cp data-start=\"555\" data-end=\"891\"\u003e\u003cstrong data-start=\"555\" data-end=\"577\"\u003eBushnell-Haas Agar\u003c\/strong\u003e is a \u003cstrong data-start=\"583\" data-end=\"615\"\u003emineral salts culture medium\u003c\/strong\u003e designed for the \u003cstrong data-start=\"633\" data-end=\"710\"\u003eisolation, enumeration, and study of hydrocarbon-degrading microorganisms\u003c\/strong\u003e. The formulation was originally developed by \u003cstrong data-start=\"756\" data-end=\"784\"\u003eBushnell and Haas (1941)\u003c\/strong\u003e and is widely used in \u003cstrong data-start=\"807\" data-end=\"890\"\u003eenvironmental microbiology, petroleum microbiology, and bioremediation research\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp data-start=\"893\" data-end=\"1267\"\u003eThe medium provides essential inorganic nutrients including \u003cstrong data-start=\"953\" data-end=\"1015\"\u003enitrogen, phosphorus, sulfur, magnesium, calcium, and iron\u003c\/strong\u003e, but \u003cstrong data-start=\"1021\" data-end=\"1057\"\u003edoes not contain a carbon source\u003c\/strong\u003e. This design ensures that microbial growth occurs only when \u003cstrong data-start=\"1118\" data-end=\"1143\"\u003ehydrocarbon compounds\u003c\/strong\u003e such as \u003cstrong data-start=\"1152\" data-end=\"1212\"\u003ekerosene, diesel, gasoline, paraffin wax, or mineral oil\u003c\/strong\u003e are supplied as the \u003cstrong data-start=\"1233\" data-end=\"1266\"\u003esole carbon and energy source\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp data-start=\"1269\" data-end=\"1608\"\u003eBushnell-Haas Agar is particularly valuable for studying the \u003cstrong data-start=\"1330\" data-end=\"1374\"\u003ebiodegradation of petroleum hydrocarbons\u003c\/strong\u003e in environmental samples such as \u003cstrong data-start=\"1408\" data-end=\"1461\"\u003esoil, water, oil spill residues, and fuel systems\u003c\/strong\u003e. The medium supports growth of hydrocarbon-utilizing bacteria including \u003cstrong data-start=\"1534\" data-end=\"1607\"\u003ePseudomonas spp., Alcanivorax spp., and other environmental degraders\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp data-start=\"1610\" data-end=\"1776\"\u003eThe neutral \u003cstrong data-start=\"1622\" data-end=\"1644\"\u003epH 7.0 environment\u003c\/strong\u003e closely mimics natural ecological conditions and enables reliable detection of microorganisms capable of metabolizing hydrocarbons.\u003c\/p\u003e\n\u003chr data-start=\"1778\" data-end=\"1781\"\u003e\n\u003ch2 data-start=\"1783\" data-end=\"1797\"\u003eApplications\u003c\/h2\u003e\n\u003cp data-start=\"1798\" data-end=\"1836\"\u003eBushnell-Haas Agar is widely used for:\u003c\/p\u003e\n\u003cp data-start=\"1838\" data-end=\"2446\"\u003e• Isolation and enumeration of \u003cstrong data-start=\"1869\" data-end=\"1903\"\u003ehydrocarbon-degrading bacteria\u003c\/strong\u003e from soil, water, fuel, and environmental samples\u003cbr data-start=\"1953\" data-end=\"1956\"\u003e• \u003cstrong data-start=\"1958\" data-end=\"1984\"\u003eBioremediation studies\u003c\/strong\u003e involving degradation of petroleum contaminants including \u003cstrong data-start=\"2043\" data-end=\"2081\"\u003eTotal Petroleum Hydrocarbons (TPH)\u003c\/strong\u003e and \u003cstrong data-start=\"2086\" data-end=\"2128\"\u003ePolycyclic Aromatic Hydrocarbons (PAH)\u003c\/strong\u003e\u003cbr data-start=\"2128\" data-end=\"2131\"\u003e• Monitoring \u003cstrong data-start=\"2144\" data-end=\"2233\"\u003emicrobial contamination in aviation fuels, diesel, lubricants, and petroleum products\u003c\/strong\u003e\u003cbr data-start=\"2233\" data-end=\"2236\"\u003e• Environmental microbiology research related to \u003cstrong data-start=\"2285\" data-end=\"2339\"\u003eoil spills and hydrocarbon biodegradation kinetics\u003c\/strong\u003e\u003cbr data-start=\"2339\" data-end=\"2342\"\u003e• Enrichment and screening of microorganisms capable of using \u003cstrong data-start=\"2404\" data-end=\"2446\"\u003ehydrocarbons as the sole carbon source\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-start=\"2448\" data-end=\"2451\"\u003e\n\u003ch2 data-start=\"2453\" data-end=\"2467\"\u003eKey Features\u003c\/h2\u003e\n\u003cp data-start=\"2468\" data-end=\"3149\"\u003e• \u003cstrong data-start=\"2470\" data-end=\"2511\"\u003eCarbon-free mineral salts formulation\u003c\/strong\u003e ensuring selective growth only when hydrocarbons are present\u003cbr data-start=\"2572\" data-end=\"2575\"\u003e• Supports growth of \u003cstrong data-start=\"2596\" data-end=\"2636\"\u003ehydrocarbon-degrading microorganisms\u003c\/strong\u003e, including \u003cem data-start=\"2648\" data-end=\"2672\"\u003ePseudomonas aeruginosa\u003c\/em\u003e and related species\u003cbr data-start=\"2692\" data-end=\"2695\"\u003e• Suitable for \u003cstrong data-start=\"2710\" data-end=\"2757\"\u003eoverlay techniques with liquid hydrocarbons\u003c\/strong\u003e or \u003cstrong data-start=\"2761\" data-end=\"2813\"\u003einverted plate methods for volatile hydrocarbons\u003c\/strong\u003e\u003cbr data-start=\"2813\" data-end=\"2816\"\u003e• Widely used in \u003cstrong data-start=\"2833\" data-end=\"2886\"\u003eASTM, EPA, and environmental monitoring protocols\u003c\/strong\u003e for petroleum microbiology\u003cbr data-start=\"2913\" data-end=\"2916\"\u003e• \u003cstrong data-start=\"2918\" data-end=\"2948\"\u003eStable pH buffering system\u003c\/strong\u003e that maintains optimal microbial growth conditions\u003cbr data-start=\"2999\" data-end=\"3002\"\u003e• \u003cstrong data-start=\"3004\" data-end=\"3064\"\u003eCharacteristic precipitate formation after sterilization\u003c\/strong\u003e (white to yellow-orange) confirms proper preparation and does not affect performance\u003c\/p\u003e\n\u003chr data-start=\"3151\" data-end=\"3154\"\u003e\n\u003ch2 data-start=\"3156\" data-end=\"3181\"\u003eComposition (per Liter)\u003c\/h2\u003e\n\u003cdiv class=\"TyagGW_tableContainer\"\u003e\n\u003cdiv class=\"group TyagGW_tableWrapper flex flex-col-reverse w-fit\" tabindex=\"-1\"\u003e\n\u003ctable data-start=\"3183\" data-end=\"3492\" class=\"w-fit min-w-(--thread-content-width)\"\u003e\n\u003cthead data-start=\"3183\" data-end=\"3205\"\u003e\n\u003ctr data-start=\"3183\" data-end=\"3205\"\u003e\n\u003cth data-start=\"3183\" data-end=\"3195\" data-col-size=\"sm\" class=\"\"\u003eComponent\u003c\/th\u003e\n\u003cth data-start=\"3195\" data-end=\"3205\" data-col-size=\"sm\" class=\"\"\u003eAmount\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody data-start=\"3216\" data-end=\"3492\"\u003e\n\u003ctr data-start=\"3216\" data-end=\"3259\"\u003e\n\u003ctd data-start=\"3216\" data-end=\"3249\" data-col-size=\"sm\"\u003eMagnesium Sulfate (MgSO₄·7H₂O)\u003c\/td\u003e\n\u003ctd data-col-size=\"sm\" data-start=\"3249\" data-end=\"3259\"\u003e0.20 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-start=\"3260\" data-end=\"3302\"\u003e\n\u003ctd data-start=\"3260\" data-end=\"3292\" data-col-size=\"sm\"\u003eCalcium Chloride (CaCl₂·2H₂O)\u003c\/td\u003e\n\u003ctd data-col-size=\"sm\" data-start=\"3292\" data-end=\"3302\"\u003e0.02 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-start=\"3303\" data-end=\"3348\"\u003e\n\u003ctd data-start=\"3303\" data-end=\"3338\" data-col-size=\"sm\"\u003eMonopotassium Phosphate (KH₂PO₄)\u003c\/td\u003e\n\u003ctd data-col-size=\"sm\" data-start=\"3338\" data-end=\"3348\"\u003e1.00 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-start=\"3349\" data-end=\"3392\"\u003e\n\u003ctd data-start=\"3349\" data-end=\"3382\" data-col-size=\"sm\"\u003eDipotassium Phosphate (K₂HPO₄)\u003c\/td\u003e\n\u003ctd data-col-size=\"sm\" data-start=\"3382\" data-end=\"3392\"\u003e1.00 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-start=\"3393\" data-end=\"3431\"\u003e\n\u003ctd data-start=\"3393\" data-end=\"3421\" data-col-size=\"sm\"\u003eAmmonium Nitrate (NH₄NO₃)\u003c\/td\u003e\n\u003ctd data-col-size=\"sm\" data-start=\"3421\" data-end=\"3431\"\u003e1.00 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-start=\"3432\" data-end=\"3473\"\u003e\n\u003ctd data-start=\"3432\" data-end=\"3463\" data-col-size=\"sm\"\u003eFerric Chloride (FeCl₃·6H₂O)\u003c\/td\u003e\n\u003ctd data-col-size=\"sm\" data-start=\"3463\" data-end=\"3473\"\u003e0.05 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-start=\"3474\" data-end=\"3492\"\u003e\n\u003ctd data-start=\"3474\" data-end=\"3481\" data-col-size=\"sm\"\u003eAgar\u003c\/td\u003e\n\u003ctd data-start=\"3481\" data-end=\"3492\" data-col-size=\"sm\"\u003e20.00 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp data-start=\"3494\" data-end=\"3526\"\u003e\u003cstrong data-start=\"3494\" data-end=\"3507\"\u003eFinal pH:\u003c\/strong\u003e 7.0 ± 0.2 at 25 °C\u003c\/p\u003e\n\u003chr data-start=\"3528\" data-end=\"3531\"\u003e\n\u003ch2 data-start=\"3533\" data-end=\"3546\"\u003ePreparation\u003c\/h2\u003e\n\u003col data-start=\"3547\" data-end=\"3832\"\u003e\n\u003cli data-start=\"3547\" data-end=\"3639\"\u003e\n\u003cp data-start=\"3550\" data-end=\"3639\"\u003eSuspend \u003cstrong data-start=\"3558\" data-end=\"3569\"\u003e23.27 g\u003c\/strong\u003e of dehydrated medium in \u003cstrong data-start=\"3594\" data-end=\"3636\"\u003e1 liter of distilled or purified water\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3640\" data-end=\"3724\"\u003e\n\u003cp data-start=\"3643\" data-end=\"3724\"\u003eHeat with gentle agitation and \u003cstrong data-start=\"3674\" data-end=\"3721\"\u003ebring to boiling until completely dissolved\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3725\" data-end=\"3774\"\u003e\n\u003cp data-start=\"3728\" data-end=\"3774\"\u003eDispense into suitable containers or flasks.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-start=\"3775\" data-end=\"3832\"\u003e\n\u003cp data-start=\"3778\" data-end=\"3832\"\u003eSterilize by \u003cstrong data-start=\"3791\" data-end=\"3831\"\u003eautoclaving at 121 °C for 15 minutes\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-start=\"3834\" data-end=\"3995\"\u003eAfter sterilization, a \u003cstrong data-start=\"3857\" data-end=\"3935\"\u003ewhite precipitate may form and may change to yellow or orange upon cooling\u003c\/strong\u003e. This is \u003cstrong data-start=\"3945\" data-end=\"3994\"\u003enormal and does not affect medium performance\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp data-start=\"3997\" data-end=\"4033\"\u003eFor hydrocarbon utilization studies:\u003c\/p\u003e\n\u003cp data-start=\"4035\" data-end=\"4258\"\u003e• Add \u003cstrong data-start=\"4041\" data-end=\"4073\"\u003e0.5–1 mL sterile hydrocarbon\u003c\/strong\u003e (e.g., mineral oil, diesel, kerosene) to the agar surface after solidification\u003cbr data-start=\"4152\" data-end=\"4155\"\u003e• Alternatively, apply \u003cstrong data-start=\"4178\" data-end=\"4233\"\u003evolatile hydrocarbons in the lid of inverted plates\u003c\/strong\u003e for vapor-phase exposure\u003c\/p\u003e\n\u003chr data-start=\"4260\" data-end=\"4263\"\u003e\n\u003ch2 data-start=\"4265\" data-end=\"4274\"\u003eStorage\u003c\/h2\u003e\n\u003ch3 data-start=\"4276\" data-end=\"4297\"\u003eDehydrated Medium\u003c\/h3\u003e\n\u003cp data-start=\"4298\" data-end=\"4392\"\u003eStore in a \u003cstrong data-start=\"4309\" data-end=\"4349\"\u003etightly closed container at 10–30 °C\u003c\/strong\u003e, protected from moisture and direct light.\u003c\/p\u003e\n\u003ch3 data-start=\"4394\" data-end=\"4413\"\u003ePrepared Plates\u003c\/h3\u003e\n\u003cp data-start=\"4414\" data-end=\"4514\"\u003eStore at \u003cstrong data-start=\"4423\" data-end=\"4433\"\u003e2–8 °C\u003c\/strong\u003e and use within \u003cstrong data-start=\"4449\" data-end=\"4460\"\u003e2 weeks\u003c\/strong\u003e.\u003cbr data-start=\"4461\" data-end=\"4464\"\u003eStore plates \u003cstrong data-start=\"4477\" data-end=\"4489\"\u003einverted\u003c\/strong\u003e to minimize dehydration.\u003c\/p\u003e\n\u003cp data-start=\"4516\" data-end=\"4631\"\u003eDiscard prepared media if:\u003cbr\u003e• Contamination is observed\u003cbr data-start=\"4570\" data-end=\"4573\"\u003e• Agar becomes dehydrated\u003cbr data-start=\"4598\" data-end=\"4601\"\u003e• Significant pH change occurs\u003c\/p\u003e\n\u003chr data-start=\"4633\" data-end=\"4636\"\u003e\n\u003ch2 data-start=\"4638\" data-end=\"4672\"\u003eQuality \u0026amp; Regulatory Information\u003c\/h2\u003e\n\u003cp data-start=\"4673\" data-end=\"4808\"\u003eBushnell-Haas Agar is manufactured under controlled conditions for \u003cstrong data-start=\"4740\" data-end=\"4782\"\u003econsistent microbiological performance\u003c\/strong\u003e and quality verification.\u003c\/p\u003e\n\u003cp data-start=\"4810\" data-end=\"4909\"\u003e\u003cstrong data-start=\"4810\" data-end=\"4822\"\u003eHS Code:\u003c\/strong\u003e 3821.00 – Prepared culture media for the development or maintenance of microorganisms.\u003c\/p\u003e\n\u003chr data-start=\"4911\" data-end=\"4914\"\u003e\n\u003ch2 data-start=\"4916\" data-end=\"4936\"\u003eSafety Information\u003c\/h2\u003e\n\u003cp data-start=\"4937\" data-end=\"5071\"\u003e⚠ \u003cstrong data-start=\"4939\" data-end=\"4991\"\u003eFor laboratory research and industrial use only.\u003c\/strong\u003e\u003cbr data-start=\"4991\" data-end=\"4994\"\u003eNot intended for human, veterinary, or pharmaceutical use without validation.\u003c\/p\u003e\n\u003cp data-start=\"5073\" data-end=\"5327\"\u003e• Handle laboratory chemicals using appropriate \u003cstrong data-start=\"5121\" data-end=\"5160\"\u003epersonal protective equipment (PPE)\u003c\/strong\u003e\u003cbr data-start=\"5160\" data-end=\"5163\"\u003e• \u003cstrong data-start=\"5165\" data-end=\"5209\"\u003eHydrocarbon supplements may be hazardous\u003c\/strong\u003e and should be handled in a \u003cstrong data-start=\"5237\" data-end=\"5274\"\u003ewell-ventilated area or fume hood\u003c\/strong\u003e\u003cbr data-start=\"5274\" data-end=\"5277\"\u003e• Autoclave contaminated materials before disposal\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"Default Title","offer_id":48030968479999,"sku":"AS-1161-500G","price":115.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/Bushnell-Haas_Agar.jpg?v=1772603887","url":"https:\/\/ausamics.com.au\/products\/bushnell-haas-agar","provider":"AuSaMicS","version":"1.0","type":"link"}