{"product_id":"bushnell-haas-broth","title":"Bushnell-Haas Broth | Hydrocarbon Degrading Bacteria Medium | Ausamics","description":"\u003ch2 data-end=\"324\" data-start=\"301\"\u003eBushnell-Haas Broth\u003c\/h2\u003e\n\u003cp data-end=\"346\" data-start=\"325\"\u003e\u003cstrong data-end=\"346\" data-start=\"325\"\u003eCat. No.: AS-1162\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch3 data-end=\"359\" data-start=\"348\"\u003eOverview\u003c\/h3\u003e\n\u003cp data-end=\"530\" data-start=\"360\"\u003e\u003cstrong data-end=\"406\" data-start=\"360\"\u003eBushnell-Haas Broth (Bushnell-Haas Medium)\u003c\/strong\u003e is a \u003cstrong data-end=\"436\" data-start=\"412\"\u003emineral salts medium\u003c\/strong\u003e used for the \u003cstrong data-end=\"527\" data-start=\"450\"\u003eisolation, enumeration, and study of hydrocarbon-degrading microorganisms\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp data-end=\"813\" data-start=\"532\"\u003eOriginally developed by \u003cstrong data-end=\"584\" data-start=\"556\"\u003eBushnell and Haas (1941)\u003c\/strong\u003e, this medium provides essential inorganic nutrients while \u003cstrong data-end=\"670\" data-start=\"643\"\u003elacking a carbon source\u003c\/strong\u003e, requiring supplementation with hydrocarbons such as \u003cstrong data-end=\"784\" data-start=\"724\"\u003emineral oil, kerosene, paraffin wax, diesel, or gasoline\u003c\/strong\u003e to support microbial growth.\u003c\/p\u003e\n\u003cp data-end=\"1069\" data-start=\"815\"\u003eThis selective formulation is widely used in \u003cstrong data-end=\"917\" data-start=\"860\"\u003eenvironmental microbiology and petroleum microbiology\u003c\/strong\u003e to evaluate microbial degradation of hydrocarbons. Growth of hydrocarbon-utilizing organisms is typically observed as \u003cstrong data-end=\"1068\" data-start=\"1036\"\u003eturbidity in liquid cultures\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp data-end=\"1249\" data-start=\"1071\"\u003eBushnell-Haas Broth supports the growth of microorganisms capable of using hydrocarbons as their \u003cstrong data-end=\"1201\" data-start=\"1168\"\u003esole carbon and energy source\u003c\/strong\u003e, including species such as \u003cstrong data-end=\"1249\" data-start=\"1229\"\u003ePseudomonas spp.\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-end=\"1254\" data-start=\"1251\"\u003e\n\u003ch3 data-end=\"1270\" data-start=\"1256\"\u003eApplications\u003c\/h3\u003e\n\u003cp data-end=\"1310\" data-start=\"1271\"\u003eBushnell-Haas Broth is widely used for:\u003c\/p\u003e\n\u003cp data-end=\"1776\" data-start=\"1312\"\u003e• Detection and enumeration of \u003cstrong data-end=\"1383\" data-start=\"1343\"\u003ehydrocarbon-utilizing microorganisms\u003c\/strong\u003e in soil, water, fuel, and oil-spill samples\u003cbr data-end=\"1430\" data-start=\"1427\"\u003e• \u003cstrong data-end=\"1459\" data-start=\"1432\"\u003eBioremediation research\u003c\/strong\u003e, including degradation studies of petroleum hydrocarbons (TPH) and PAHs\u003cbr data-end=\"1534\" data-start=\"1531\"\u003e• \u003cstrong data-end=\"1571\" data-start=\"1536\"\u003eMicrobiological quality control\u003c\/strong\u003e of aviation fuels, lubricants, and petroleum-based products\u003cbr data-end=\"1634\" data-start=\"1631\"\u003e• Environmental monitoring of \u003cstrong data-end=\"1686\" data-start=\"1664\"\u003efuel contamination\u003c\/strong\u003e and microbial degradation\u003cbr data-end=\"1715\" data-start=\"1712\"\u003e• Studies on \u003cstrong data-end=\"1776\" data-start=\"1728\"\u003emicrobial ecology and hydrocarbon metabolism\u003c\/strong\u003e\u003c\/p\u003e\n\u003chr data-end=\"1781\" data-start=\"1778\"\u003e\n\u003ch3 data-end=\"1797\" data-start=\"1783\"\u003eKey Features\u003c\/h3\u003e\n\u003cp data-end=\"2343\" data-start=\"1798\"\u003e• \u003cstrong data-end=\"1841\" data-start=\"1800\"\u003eCarbon-free mineral salts formulation\u003c\/strong\u003e ensuring selective growth only in the presence of hydrocarbons\u003cbr data-end=\"1907\" data-start=\"1904\"\u003e• Provides essential nutrients including \u003cstrong data-end=\"2002\" data-start=\"1948\"\u003enitrogen, phosphorus, magnesium, calcium, and iron\u003c\/strong\u003e\u003cbr data-end=\"2005\" data-start=\"2002\"\u003e• Supports growth of \u003cstrong data-end=\"2060\" data-start=\"2026\"\u003ehydrocarbon-degrading bacteria\u003c\/strong\u003e such as \u003cem data-end=\"2093\" data-start=\"2069\"\u003ePseudomonas aeruginosa\u003c\/em\u003e\u003cbr data-end=\"2096\" data-start=\"2093\"\u003e• Suitable for \u003cstrong data-end=\"2158\" data-start=\"2111\"\u003eenrichment culture and turbidimetric assays\u003c\/strong\u003e\u003cbr data-end=\"2161\" data-start=\"2158\"\u003e• Widely used in \u003cstrong data-end=\"2224\" data-start=\"2178\"\u003eASTM, EPA, and fuel microbiology protocols\u003c\/strong\u003e\u003cbr data-end=\"2227\" data-start=\"2224\"\u003e• \u003cstrong data-end=\"2283\" data-start=\"2229\"\u003eNormal yellow-orange precipitate after autoclaving\u003c\/strong\u003e confirms proper preparation and does not affect performance\u003c\/p\u003e\n\u003chr data-end=\"2348\" data-start=\"2345\"\u003e\n\u003ch3 data-end=\"2375\" data-start=\"2350\"\u003eComposition (per liter)\u003c\/h3\u003e\n\u003cdiv class=\"TyagGW_tableContainer\"\u003e\n\u003cdiv class=\"group TyagGW_tableWrapper flex flex-col-reverse w-fit\" tabindex=\"-1\"\u003e\n\u003ctable class=\"w-fit min-w-(--thread-content-width)\" data-end=\"2674\" data-start=\"2377\"\u003e\n\u003cthead data-end=\"2400\" data-start=\"2377\"\u003e\n\u003ctr data-end=\"2400\" data-start=\"2377\"\u003e\n\u003cth class=\"\" data-col-size=\"sm\" data-end=\"2390\" data-start=\"2377\"\u003eIngredient\u003c\/th\u003e\n\u003cth class=\"\" data-col-size=\"sm\" data-end=\"2400\" data-start=\"2390\"\u003eAmount\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody data-end=\"2674\" data-start=\"2411\"\u003e\n\u003ctr data-end=\"2454\" data-start=\"2411\"\u003e\n\u003ctd data-col-size=\"sm\" data-end=\"2444\" data-start=\"2411\"\u003eMagnesium sulfate (MgSO₄·7H₂O)\u003c\/td\u003e\n\u003ctd data-end=\"2454\" data-start=\"2444\" data-col-size=\"sm\"\u003e0.20 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-end=\"2503\" data-start=\"2455\"\u003e\n\u003ctd data-col-size=\"sm\" data-end=\"2493\" data-start=\"2455\"\u003eCalcium chloride, anhydrous (CaCl₂)\u003c\/td\u003e\n\u003ctd data-end=\"2503\" data-start=\"2493\" data-col-size=\"sm\"\u003e0.02 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-end=\"2549\" data-start=\"2504\"\u003e\n\u003ctd data-col-size=\"sm\" data-end=\"2539\" data-start=\"2504\"\u003eMonopotassium phosphate (KH₂PO₄)\u003c\/td\u003e\n\u003ctd data-end=\"2549\" data-start=\"2539\" data-col-size=\"sm\"\u003e1.00 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-end=\"2593\" data-start=\"2550\"\u003e\n\u003ctd data-col-size=\"sm\" data-end=\"2583\" data-start=\"2550\"\u003eDipotassium phosphate (K₂HPO₄)\u003c\/td\u003e\n\u003ctd data-end=\"2593\" data-start=\"2583\" data-col-size=\"sm\"\u003e1.00 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-end=\"2632\" data-start=\"2594\"\u003e\n\u003ctd data-col-size=\"sm\" data-end=\"2622\" data-start=\"2594\"\u003eAmmonium nitrate (NH₄NO₃)\u003c\/td\u003e\n\u003ctd data-end=\"2632\" data-start=\"2622\" data-col-size=\"sm\"\u003e1.00 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-end=\"2674\" data-start=\"2633\"\u003e\n\u003ctd data-col-size=\"sm\" data-end=\"2664\" data-start=\"2633\"\u003eFerric chloride (FeCl₃·6H₂O)\u003c\/td\u003e\n\u003ctd data-end=\"2674\" data-start=\"2664\" data-col-size=\"sm\"\u003e0.05 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp data-end=\"2708\" data-start=\"2676\"\u003e\u003cstrong data-end=\"2689\" data-start=\"2676\"\u003eFinal pH:\u003c\/strong\u003e 7.0 ± 0.2 at 25 °C\u003c\/p\u003e\n\u003chr data-end=\"2713\" data-start=\"2710\"\u003e\n\u003ch3 data-end=\"2728\" data-start=\"2715\"\u003ePreparation\u003c\/h3\u003e\n\u003col data-end=\"2994\" data-start=\"2729\"\u003e\n\u003cli data-end=\"2820\" data-start=\"2729\"\u003e\n\u003cp data-end=\"2820\" data-start=\"2732\"\u003eSuspend \u003cstrong data-end=\"2750\" data-start=\"2740\"\u003e3.27 g\u003c\/strong\u003e of dehydrated medium in \u003cstrong data-end=\"2786\" data-start=\"2775\"\u003e1 liter\u003c\/strong\u003e of distilled or purified water.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"2889\" data-start=\"2821\"\u003e\n\u003cp data-end=\"2889\" data-start=\"2824\"\u003eHeat gently with frequent agitation until completely dissolved.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"2934\" data-start=\"2890\"\u003e\n\u003cp data-end=\"2934\" data-start=\"2893\"\u003eDispense into suitable tubes or flasks.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"2994\" data-start=\"2935\"\u003e\n\u003cp data-end=\"2994\" data-start=\"2938\"\u003eSterilize by \u003cstrong data-end=\"2991\" data-start=\"2951\"\u003eautoclaving at 121 °C for 15 minutes\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp data-end=\"3131\" data-start=\"2996\"\u003eAfter sterilization and cooling, a \u003cstrong data-end=\"3074\" data-start=\"3031\"\u003eyellow-to-orange precipitate may appear\u003c\/strong\u003e, which is normal and does not affect medium performance.\u003c\/p\u003e\n\u003cp data-end=\"3267\" data-start=\"3133\"\u003eFor use, \u003cstrong data-end=\"3190\" data-start=\"3142\"\u003easeptically add a sterile hydrocarbon source\u003c\/strong\u003e (e.g., mineral oil, kerosene, diesel, or paraffin) and inoculate the sample.\u003c\/p\u003e\n\u003cp data-end=\"3351\" data-start=\"3269\"\u003ePrepared medium appears \u003cstrong data-end=\"3350\" data-start=\"3293\"\u003ecolorless to slightly opalescent with slight sediment\u003c\/strong\u003e.\u003c\/p\u003e\n\u003chr data-end=\"3356\" data-start=\"3353\"\u003e\n\u003ch3 data-end=\"3367\" data-start=\"3358\"\u003eStorage\u003c\/h3\u003e\n\u003ch3 data-end=\"3390\" data-start=\"3369\"\u003eDehydrated Medium\u003c\/h3\u003e\n\u003cp data-end=\"3485\" data-start=\"3391\"\u003eStore in a \u003cstrong data-end=\"3442\" data-start=\"3402\"\u003etightly closed container at 10–30 °C\u003c\/strong\u003e, protected from moisture and direct light.\u003c\/p\u003e\n\u003ch3 data-end=\"3506\" data-start=\"3487\"\u003ePrepared Medium\u003c\/h3\u003e\n\u003cp data-end=\"3554\" data-start=\"3507\"\u003eStore at \u003cstrong data-end=\"3526\" data-start=\"3516\"\u003e2–8 °C\u003c\/strong\u003e and use within \u003cstrong data-end=\"3553\" data-start=\"3542\"\u003e2 weeks\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp data-end=\"3662\" data-start=\"3556\"\u003eDiscard if:\u003cbr\u003e• Uninoculated broth becomes turbid\u003cbr data-end=\"3606\" data-start=\"3603\"\u003e• Contamination is observed\u003cbr data-end=\"3636\" data-start=\"3633\"\u003e• pH changes significantly\u003c\/p\u003e\n\u003chr data-end=\"3667\" data-start=\"3664\"\u003e\n\u003ch3 data-end=\"3687\" data-start=\"3669\"\u003eQuality \u0026amp; Safety\u003c\/h3\u003e\n\u003cp data-end=\"3848\" data-start=\"3688\"\u003e• Manufactured under controlled conditions for \u003cstrong data-end=\"3777\" data-start=\"3735\"\u003econsistent microbiological performance\u003c\/strong\u003e\u003cbr data-end=\"3780\" data-start=\"3777\"\u003e• Each batch tested according to \u003cstrong data-end=\"3848\" data-start=\"3813\"\u003einternal quality specifications\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-end=\"4025\" data-start=\"3850\"\u003e⚠ \u003cstrong data-end=\"3904\" data-start=\"3852\"\u003eFor laboratory research and industrial use only.\u003c\/strong\u003e\u003cbr data-end=\"3907\" data-start=\"3904\"\u003eNot intended for food, pharmaceutical, or veterinary applications.\u003cbr data-end=\"3976\" data-start=\"3973\"\u003eNot for diagnostic use without proper validation.\u003c\/p\u003e\n\u003cp data-end=\"4194\" data-start=\"4027\"\u003eWhen handling hydrocarbon supplements, work in a \u003cstrong data-end=\"4113\" data-start=\"4076\"\u003ewell-ventilated area or fume hood\u003c\/strong\u003e. Dispose of contaminated materials according to laboratory biosafety procedures.\u003c\/p\u003e\n\u003chr data-end=\"4199\" data-start=\"4196\"\u003e\n\u003ch3 data-start=\"129\" data-end=\"153\"\u003e\u003cstrong data-start=\"133\" data-end=\"153\"\u003eHS Code: 3821.00\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp data-start=\"154\" data-end=\"263\"\u003ePrepared culture media for development or maintenance of microorganisms.\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"Default Title","offer_id":48030968512767,"sku":"AS-1162-500G","price":115.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/Bushnell-Haas_Agar.jpg?v=1772603887","url":"https:\/\/ausamics.com.au\/products\/bushnell-haas-broth","provider":"AuSaMicS","version":"1.0","type":"link"}