{"product_id":"columbia-blood-agar-base","title":"Columbia Blood Agar Base","description":"\u003cp data-start=\"199\" data-end=\"299\"\u003e\u003cstrong data-start=\"199\" data-end=\"227\"\u003eColumbia Blood Agar Base\u003c\/strong\u003e\u003cbr data-start=\"227\" data-end=\"230\"\u003eEnriched Blood Agar Base for Fastidious and Non-Fastidious Bacteria\u003c\/p\u003e\n\u003cp data-start=\"301\" data-end=\"330\"\u003e\u003cstrong data-start=\"301\" data-end=\"320\"\u003eCatalog Number:\u003c\/strong\u003e AS-1180\u003c\/p\u003e\n\u003chr data-start=\"332\" data-end=\"335\"\u003e\n\u003cp data-start=\"337\" data-end=\"698\"\u003e\u003cstrong data-start=\"337\" data-end=\"349\"\u003eOverview\u003c\/strong\u003e\u003cbr data-start=\"349\" data-end=\"352\"\u003eColumbia Blood Agar Base, when supplemented with 5–10% defibrinated sheep blood, produces Columbia Blood Agar—a highly enriched, non-selective solid medium for the primary isolation, cultivation, and characterisation of fastidious and non-fastidious microorganisms from clinical specimens such as blood, throat swabs, wounds, and genital samples.\u003c\/p\u003e\n\u003cp data-start=\"700\" data-end=\"1178\"\u003eFormulated with specialised Columbia peptone (a refined meat digest) and enriched with yeast and beef extracts, the medium supports superior growth and colony development. Blood supplementation enables clear visualisation of haemolytic reactions (alpha, beta, gamma), critical for presumptive identification of streptococci, staphylococci, and other clinically relevant organisms. Corn starch neutralises toxic metabolites, improving recovery of stressed or low-viability cells.\u003c\/p\u003e\n\u003chr data-start=\"1180\" data-end=\"1183\"\u003e\n\u003cp data-start=\"1185\" data-end=\"1689\"\u003e\u003cstrong data-start=\"1185\" data-end=\"1201\"\u003eApplications\u003c\/strong\u003e\u003cbr data-start=\"1201\" data-end=\"1204\"\u003e• Isolation of beta-haemolytic streptococci such as \u003cem data-start=\"1256\" data-end=\"1280\"\u003eStreptococcus pyogenes\u003c\/em\u003e and \u003cem data-start=\"1285\" data-end=\"1300\"\u003eS. agalactiae\u003c\/em\u003e from throat and respiratory specimens\u003cbr data-start=\"1338\" data-end=\"1341\"\u003e• Cultivation of \u003cem data-start=\"1358\" data-end=\"1382\"\u003eHaemophilus influenzae\u003c\/em\u003e, \u003cem data-start=\"1384\" data-end=\"1408\"\u003eNeisseria meningitidis\u003c\/em\u003e, and viridans streptococci from blood, CSF, and respiratory samples\u003cbr data-start=\"1476\" data-end=\"1479\"\u003e• Observation of haemolysis patterns for presumptive identification in antimicrobial susceptibility workflows\u003cbr data-start=\"1588\" data-end=\"1591\"\u003e• General-purpose enriched medium for mixed flora in wound, urine, and food microbiology testing\u003c\/p\u003e\n\u003chr data-start=\"1691\" data-end=\"1694\"\u003e\n\u003cp data-start=\"1696\" data-end=\"2103\"\u003e\u003cstrong data-start=\"1696\" data-end=\"1723\"\u003eKey Features \u0026amp; Benefits\u003c\/strong\u003e\u003cbr data-start=\"1723\" data-end=\"1726\"\u003e• Columbia peptone enhances colony size, morphology, and pigment production\u003cbr data-start=\"1801\" data-end=\"1804\"\u003e• Blood supplementation provides X-factor (hemin) and enables clear haemolysis\u003cbr data-start=\"1882\" data-end=\"1885\"\u003e• Corn starch buffers toxic by-products and stabilises growth conditions\u003cbr data-start=\"1957\" data-end=\"1960\"\u003e• Produces well-defined haemolytic zones without distortion from overgrowth\u003cbr data-start=\"2035\" data-end=\"2038\"\u003e• Suitable for aerobic and CO₂-enriched incubation environments\u003c\/p\u003e\n\u003chr data-start=\"2105\" data-end=\"2108\"\u003e\n\u003cp data-start=\"2110\" data-end=\"2541\"\u003e\u003cstrong data-start=\"2110\" data-end=\"2137\"\u003ePrinciple of the Medium\u003c\/strong\u003e\u003cbr data-start=\"2137\" data-end=\"2140\"\u003eColumbia Blood Agar Base supplies a rich nutrient environment through refined meat digests and yeast extract, promoting robust bacterial growth. Addition of defibrinated sheep blood provides essential growth factors and allows differentiation of organisms based on haemolytic activity. Corn starch absorbs inhibitory substances, enhancing recovery of fastidious organisms and improving colony clarity.\u003c\/p\u003e\n\u003chr data-start=\"2543\" data-end=\"2546\"\u003e\n\u003cp data-start=\"2548\" data-end=\"2776\"\u003e\u003cstrong data-start=\"2548\" data-end=\"2583\"\u003eTypical Composition (per litre)\u003c\/strong\u003e\u003cbr data-start=\"2583\" data-end=\"2586\"\u003ePancreatic Digest of Casein – 12.0 g\u003cbr data-start=\"2622\" data-end=\"2625\"\u003ePeptic Digest of Animal Tissue – 5.0 g\u003cbr data-start=\"2663\" data-end=\"2666\"\u003eYeast Extract – 3.0 g\u003cbr data-start=\"2687\" data-end=\"2690\"\u003eBeef Extract – 3.0 g\u003cbr data-start=\"2710\" data-end=\"2713\"\u003eCorn Starch – 1.0 g\u003cbr data-start=\"2732\" data-end=\"2735\"\u003eSodium Chloride – 5.0 g\u003cbr data-start=\"2758\" data-end=\"2761\"\u003eAgar – 13.5 g\u003c\/p\u003e\n\u003cp data-start=\"2778\" data-end=\"2866\"\u003eSupplement (added after sterilisation):\u003cbr data-start=\"2817\" data-end=\"2820\"\u003eDefibrinated Sheep Blood – 50–100 mL (5–10%)\u003c\/p\u003e\n\u003cp data-start=\"2868\" data-end=\"2898\"\u003eFinal pH: 7.3 ± 0.2 at 25 °C\u003c\/p\u003e\n\u003chr data-start=\"2900\" data-end=\"2903\"\u003e\n\u003cp data-start=\"2905\" data-end=\"3416\"\u003e\u003cstrong data-start=\"2905\" data-end=\"2920\"\u003ePreparation\u003c\/strong\u003e\u003cbr data-start=\"2920\" data-end=\"2923\"\u003eSuspend 39.5 g of dehydrated base in 950 mL purified or distilled water.\u003cbr data-start=\"2995\" data-end=\"2998\"\u003eHeat with gentle agitation to boiling until completely dissolved; do not overheat.\u003cbr data-start=\"3080\" data-end=\"3083\"\u003eSterilise by autoclaving at 121 °C (15 psi) for 15 minutes.\u003cbr data-start=\"3142\" data-end=\"3145\"\u003eCool to 45–50 °C in a water bath.\u003cbr data-start=\"3178\" data-end=\"3181\"\u003eAseptically add 50–100 mL sterile defibrinated sheep blood and mix gently to avoid haemolysis.\u003cbr data-start=\"3275\" data-end=\"3278\"\u003ePour into sterile Petri dishes (20–25 mL per plate) or dispense as required.\u003cbr data-start=\"3354\" data-end=\"3357\"\u003eAllow to solidify and dry briefly under aseptic conditions.\u003c\/p\u003e\n\u003cp data-start=\"3418\" data-end=\"3521\"\u003eThe prepared medium should appear opaque, reddish-brown, and homogeneous without bubbles or separation.\u003c\/p\u003e\n\u003chr data-start=\"3523\" data-end=\"3526\"\u003e\n\u003cp data-start=\"3528\" data-end=\"3684\"\u003e\u003cstrong data-start=\"3528\" data-end=\"3542\"\u003eIncubation\u003c\/strong\u003e\u003cbr data-start=\"3542\" data-end=\"3545\"\u003eInoculate using a calibrated loop or sterile swab.\u003cbr data-start=\"3595\" data-end=\"3598\"\u003eIncubate at 35–37 °C aerobically or in 5% CO₂.\u003cbr data-start=\"3644\" data-end=\"3647\"\u003eTypical incubation time: 18–48 hours.\u003c\/p\u003e\n\u003chr data-start=\"3686\" data-end=\"3689\"\u003e\n\u003cp data-start=\"3691\" data-end=\"3993\"\u003e\u003cstrong data-start=\"3691\" data-end=\"3714\"\u003eStorage \u0026amp; Stability\u003c\/strong\u003e\u003cbr data-start=\"3714\" data-end=\"3717\"\u003eDehydrated medium: Store at 10–30 °C in a tightly closed container, protected from moisture and light\u003cbr data-start=\"3818\" data-end=\"3821\"\u003ePrepared plates: Store at 2–8 °C in sealed plastic bags and use within 4–8 weeks\u003cbr data-start=\"3901\" data-end=\"3904\"\u003eDiscard plates that are dehydrated, haemolysed, discoloured, contaminated, or overgrown\u003c\/p\u003e\n\u003chr data-start=\"3995\" data-end=\"3998\"\u003e\n\u003cp data-start=\"4000\" data-end=\"4188\"\u003e\u003cstrong data-start=\"4000\" data-end=\"4016\"\u003eIntended Use\u003c\/strong\u003e\u003cbr data-start=\"4016\" data-end=\"4019\"\u003eFor laboratory research and microbiological quality control use only.\u003cbr data-start=\"4088\" data-end=\"4091\"\u003eNot for human or veterinary consumption.\u003cbr data-start=\"4131\" data-end=\"4134\"\u003eNot for diagnostic use without appropriate validation.\u003c\/p\u003e\n\u003chr data-start=\"4190\" data-end=\"4193\"\u003e\n\u003cp data-start=\"4195\" data-end=\"4451\"\u003e\u003cstrong data-start=\"4195\" data-end=\"4219\"\u003eQuality \u0026amp; Compliance\u003c\/strong\u003e\u003cbr data-start=\"4219\" data-end=\"4222\"\u003eManufactured under controlled conditions to ensure batch-to-batch consistency.\u003cbr data-start=\"4300\" data-end=\"4303\"\u003eSuitable for clinical, food, and environmental microbiology laboratories.\u003cbr data-start=\"4376\" data-end=\"4379\"\u003ePerformance comparable to leading international blood agar formulations.\u003c\/p\u003e\n\u003chr data-start=\"4453\" data-end=\"4456\"\u003e\n\u003cp data-start=\"4458\" data-end=\"4599\"\u003e\u003cstrong data-start=\"4458\" data-end=\"4489\"\u003eCustoms \u0026amp; Trade Information\u003c\/strong\u003e\u003cbr data-start=\"4489\" data-end=\"4492\"\u003eHS \/ AHECC Code: 3821.00.00\u003cbr data-start=\"4519\" data-end=\"4522\"\u003ePrepared culture media for the development or maintenance of microorganisms\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"100 g","offer_id":48147265290495,"sku":"AS-1180-100G","price":35.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/culturemedia_image_196e49b8-e43e-4dd5-a890-73204d0f4ce1.jpg?v=1771063012","url":"https:\/\/ausamics.com.au\/products\/columbia-blood-agar-base","provider":"AuSaMicS","version":"1.0","type":"link"}