{"product_id":"e-coli-cloning-blue-white-screening-media-set","title":"E. coli Cloning \u0026 Blue-White Screening Media Set","description":"\u003c!-- ============================================================\n     AuSaMicS — E. coli Cloning \u0026 Blue-White Screening Media Set\n     Shopify Product Page HTML Embed\n     Version: 2.1 DRAFT | ausamics.com.au\n     Workflow-driven, configurable set (not a tiered bundle)\n     ============================================================\n     RECOMMENDED SHOPIFY ADMIN SETTINGS FOR THIS PRODUCT:\n     Product page title: E. coli Cloning \u0026 Blue-White Screening Media Set\n     Meta title: E. coli Cloning \u0026 Blue-White Screening Media Set | AuSaMicS\n     Meta description: Australian-made E. coli cloning media set with LB\n       Agar, LB Broth, IPTG and X-Gal. Configure antibiotic selection and\n       optional SOC recovery medium.\n     URL handle: e-coli-cloning-blue-white-screening-media-set\n     (i.e. ausamics.com.au\/products\/e-coli-cloning-blue-white-screening-media-set)\n     ============================================================ --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- ============================================================ STYLES --\u003e\n\u003cstyle\u003e\n  .sal-wrap *{box-sizing:border-box;margin:0;padding:0;}\n  .sal-wrap{font-family:'Segoe UI',system-ui,-apple-system,sans-serif;color:#1a1a1a;line-height:1.65;max-width:960px;}\n\n  .sal-hero{background:linear-gradient(135deg,#f0f7f4 0%,#e8f4ef 100%);border:1px solid #c8e6d4;border-radius:14px;padding:28px 30px;margin-bottom:28px;}\n  .sal-hero-badge{display:inline-block;background:#1A4731;color:#fff;font-size:11px;font-weight:700;letter-spacing:.08em;text-transform:uppercase;padding:5px 13px;border-radius:20px;margin-bottom:14px;}\n  .sal-hero 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.sal-hero h1{font-size:20px;}\n    .sal-price{font-size:26px;}\n    .why-grid2{grid-template-columns:1fr 1fr;}\n    .upsell-grid2{grid-template-columns:1fr 1fr;}\n    .sal-workflow{flex-direction:column;align-items:stretch;}\n    .sal-wf-arrow{transform:rotate(90deg);align-self:center;}\n  }\n  @media(max-width:480px){\n    .why-grid2{grid-template-columns:1fr;}\n    .upsell-grid2{grid-template-columns:1fr;}\n  }\n\u003c\/style\u003e\n\u003c!-- ============================================================ HTML --\u003e\n\u003cdiv class=\"sal-wrap\"\u003e\n\u003c!-- HERO --\u003e\n\u003cdiv class=\"sal-hero\"\u003e\n\u003cdiv class=\"sal-hero-badge\"\u003eE. coli Cloning Media — Australian Made\u003c\/div\u003e\n\u003ch1\u003eE. coli Cloning \u0026amp; Blue-White Screening Media Set\u003c\/h1\u003e\n\u003cp\u003eA coordinated set of culture media and screening reagents for commonly used \u003cem\u003eE. coli\u003c\/em\u003e cloning, transformation and blue-white screening workflows. Antibiotic selection is configurable to your vector's resistance marker. Full documentation on every item.\u003c\/p\u003e\n\u003cdiv class=\"sal-trust\"\u003e\n\u003cspan class=\"sal-trust-pill\"\u003e\u003cspan class=\"sal-trust-dot\"\u003e\u003c\/span\u003eManufactured in Melbourne\u003c\/span\u003e \u003cspan class=\"sal-trust-pill\"\u003e\u003cspan class=\"sal-trust-dot\"\u003e\u003c\/span\u003eFast Australian dispatch\u003c\/span\u003e \u003cspan class=\"sal-trust-pill\"\u003e\u003cspan class=\"sal-trust-dot\"\u003e\u003c\/span\u003eSDS · TDS · COA per product\u003c\/span\u003e \u003cspan class=\"sal-trust-pill\"\u003e\u003cspan class=\"sal-trust-dot\"\u003e\u003c\/span\u003eLOT \u0026amp; EXP traceability\u003c\/span\u003e \u003cspan class=\"sal-trust-pill\"\u003e\u003cspan class=\"sal-trust-dot\"\u003e\u003c\/span\u003eDirect formulator support\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- WORKFLOW STRIP --\u003e\n\u003cp class=\"sec-title2\"\u003eWhere each product fits in the workflow\u003c\/p\u003e\n\u003cdiv class=\"sal-workflow\"\u003e\n\u003cdiv class=\"sal-wf-step\"\u003e\n\u003cstrong\u003e1. Transformation\u003c\/strong\u003e\u003cspan\u003eCompetent cells + plasmid\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cspan class=\"sal-wf-arrow\"\u003e→\u003c\/span\u003e\n\u003cdiv class=\"sal-wf-step\"\u003e\n\u003cstrong\u003e2. Recovery\u003c\/strong\u003e\u003cspan\u003eSOC Medium (optional)\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cspan class=\"sal-wf-arrow\"\u003e→\u003c\/span\u003e\n\u003cdiv class=\"sal-wf-step\"\u003e\n\u003cstrong\u003e3. Selection\/Plating\u003c\/strong\u003e\u003cspan\u003eLB Agar + chosen antibiotic\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cspan class=\"sal-wf-arrow\"\u003e→\u003c\/span\u003e\n\u003cdiv class=\"sal-wf-step\"\u003e\n\u003cstrong\u003e4. Blue-white screening\u003c\/strong\u003e\u003cspan\u003eIPTG + X-Gal\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cspan class=\"sal-wf-arrow\"\u003e→\u003c\/span\u003e\n\u003cdiv class=\"sal-wf-step\"\u003e\n\u003cstrong\u003e5. Culture\u003c\/strong\u003e\u003cspan\u003eLB Broth\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- SINGLE PRODUCT --\u003e\n\u003cdiv class=\"sal-product\"\u003e\n\u003cdiv class=\"sal-product-name\"\u003eE. coli Cloning \u0026amp; Blue-White Screening Media Set\u003c\/div\u003e\n\u003cdiv class=\"sal-product-sub\"\u003eCulture media and screening reagents for routine E. coli cloning, transformation and blue-white screening. Choose the selection antibiotic that matches your vector's resistance marker, and add SOC Medium if your protocol requires a recovery step.\u003c\/div\u003e\n\n\u003cdiv class=\"sal-price-block\"\u003e\n\u003cspan class=\"sal-price\"\u003e$260\u003c\/span\u003e \u003cspan class=\"sal-was\"\u003eCore set · Save $14 vs $274 individually\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"sal-configured-total\"\u003eConfigured total: \u003cspan id=\"ecl-total-price\"\u003e$287\u003c\/span\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"sal-items-title\"\u003eCore set — included\u003c\/div\u003e\n\u003cul class=\"sal-items\"\u003e\n\u003cli class=\"media\"\u003eLB Agar (Lennox) 500 g — AS-1268\u003c\/li\u003e\n\u003cli class=\"media\"\u003eLB Broth (Lennox) 500 g — AS-1269\u003c\/li\u003e\n\u003cli class=\"screen\"\u003eIPTG 1 g — ASE-901-1\u003c\/li\u003e\n\u003cli class=\"screen\"\u003eX-Gal 1 g — ASE-906-1G\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cdiv class=\"sal-additive-callout\"\u003e\n\u003cspan class=\"sal-additive-icon\"\u003e⚠\u003c\/span\u003e\n\u003cspan class=\"sal-additive-text\"\u003e\u003cstrong\u003eBlue-white screening requires a compatible lacZ-based host\/vector system.\u003c\/strong\u003e IPTG and X-Gal alone do not produce blue-white screening in an incompatible system. Confirm that your host strain and cloning vector support α-complementation before use.\u003c\/span\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"sal-items-title\"\u003eChoose your selection antibiotic\u003c\/div\u003e\n\u003cdiv class=\"sal-configure-link\" style=\"margin:0 0 8px;\"\u003eSelect the antibiotic that matches your vector's resistance marker.\u003c\/div\u003e\n\u003clabel class=\"sal-addon-row\"\u003e\n\u003cinput type=\"radio\" name=\"ecl-antibiotic\" value=\"27\" id=\"ecl-ab-amp\" checked\u003e\n\u003cspan\u003e\u003cstrong\u003eAmpicillin 1 g\u003c\/strong\u003e (+$27) — ASA-2044-1, for vectors carrying an ampicillin-resistance marker.\u003c\/span\u003e\n\u003c\/label\u003e\n\u003clabel class=\"sal-addon-row\"\u003e\n\u003cinput type=\"radio\" name=\"ecl-antibiotic\" value=\"25\" id=\"ecl-ab-kan\"\u003e\n\u003cspan\u003e\u003cstrong\u003eKanamycin 1 g\u003c\/strong\u003e (+$25) — ASA-2014-1, for vectors carrying a kanamycin-resistance marker.\u003c\/span\u003e\n\u003c\/label\u003e\n\u003clabel class=\"sal-addon-row\"\u003e\n\u003cinput type=\"radio\" name=\"ecl-antibiotic\" value=\"39\" id=\"ecl-ab-cam\"\u003e\n\u003cspan\u003e\u003cstrong\u003eChloramphenicol 5 g\u003c\/strong\u003e (+$39) — ASA-2042-5, for vectors or systems requiring chloramphenicol selection.\u003c\/span\u003e\n\u003c\/label\u003e\n\u003clabel class=\"sal-addon-row\"\u003e\n\u003cinput type=\"radio\" name=\"ecl-antibiotic\" value=\"0\" id=\"ecl-ab-none\"\u003e\n\u003cspan\u003e\u003cstrong\u003eNo antibiotic required\u003c\/strong\u003e — I already have my selection reagent.\u003c\/span\u003e\n\u003c\/label\u003e\n\u003cdiv class=\"sal-configure-link\" style=\"margin-top:0;\"\u003eSelection depends on the resistance marker carried by your vector\/plasmid — check your vector map before ordering.\u003c\/div\u003e\n\n\u003cdiv class=\"sal-items-title\" style=\"margin-top:14px;\"\u003eOptional transformation recovery\u003c\/div\u003e\n\u003clabel class=\"sal-addon-row\"\u003e\n\u003cinput type=\"checkbox\" id=\"ecl-soc-checkbox\"\u003e\n\u003cspan\u003e\u003cstrong\u003eAdd SOC Medium 500 g\u003c\/strong\u003e (+$85) — AS-1428-500. Rich recovery medium for the post-transformation recovery step following heat shock or electroporation.\u003c\/span\u003e\n\u003c\/label\u003e\n\n\u003ca href=\"#\" id=\"ecl-cta-top\" class=\"sal-btn-cta\" style=\"margin-top:14px;\"\u003eAdd Cloning Media Set — $287\u003c\/a\u003e\n\n\u003cdiv class=\"sal-configure-link\"\u003eNeed different pack sizes or quantities? \u003ca href=\"https:\/\/ausamics.com.au\/pages\/request-a-quote\"\u003eConfigure this set for your laboratory →\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- LEGEND --\u003e\n\u003cdiv class=\"sal-legend\"\u003e\n\u003cspan style=\"color: #6b7280; font-weight: 600; font-size: 12px;\"\u003eColour key:\u003c\/span\u003e \u003cspan style=\"background: #f0f7f4; color: #1a4731; border: 1px solid #c8e6d4; border-radius: 20px; padding: 3px 10px; font-size: 11px;\"\u003eCulture media\u003c\/span\u003e \u003cspan style=\"background: #eff6ff; color: #1e40af; border: 1px solid #bfdbfe; border-radius: 20px; padding: 3px 10px; font-size: 11px;\"\u003eScreening substrates\u003c\/span\u003e\n\u003c\/div\u003e\n\n\u003c!-- PRODUCT MATRIX --\u003e\n\u003cp class=\"sec-title2\"\u003eExact products \u0026amp; quantities — core set\u003c\/p\u003e\n\u003cdiv class=\"matrix-wrap2\"\u003e\n\u003ctable class=\"mat-table2\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct\u003c\/th\u003e\n\u003cth\u003eCode\u003c\/th\u003e\n\u003cth\u003ePack size\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eLB Agar (Lennox)\u003c\/td\u003e\n\u003ctd\u003eAS-1268\u003c\/td\u003e\n\u003ctd\u003e500 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLB Broth (Lennox)\u003c\/td\u003e\n\u003ctd\u003eAS-1269\u003c\/td\u003e\n\u003ctd\u003e500 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIPTG\u003c\/td\u003e\n\u003ctd\u003eASE-901-1\u003c\/td\u003e\n\u003ctd\u003e1 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eX-Gal\u003c\/td\u003e\n\u003ctd\u003eASE-906-1G\u003c\/td\u003e\n\u003ctd\u003e1 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp class=\"mat-footnote\"\u003eSelection antibiotic and SOC Medium are configured above and added as separate line items. LB Miller formulation (AS-1267, AS-1270) is available individually if preferred over Lennox — contact support@ausamics.com. Bulk pack sizes and custom quantities available on request.\u003c\/p\u003e\n\n\u003c!-- COMPLEMENTARY: HIGH-DENSITY CULTURE --\u003e\n\u003cp class=\"sec-title2\"\u003eHigh-density culture \u0026amp; expression\u003c\/p\u003e\n\u003cdiv class=\"upsell-grid2\"\u003e\n\u003cdiv class=\"upsell-card2\"\u003e\n\u003cdiv class=\"upsell-cat2\"\u003eHigh-Yield Media\u003c\/div\u003e\n\u003cdiv class=\"upsell-name2\"\u003eTerrific Broth (TB)\u003c\/div\u003e\n\u003cdiv class=\"upsell-code2\"\u003eAS-1429\u003c\/div\u003e\n\u003cdiv class=\"upsell-desc2\"\u003ePhosphate-buffered medium supporting high-density E. coli culture, plasmid propagation, and recombinant protein expression workflows.\u003c\/div\u003e\n\u003cdiv class=\"upsell-price-row2\"\u003e\n\u003cspan class=\"upsell-price2\"\u003e$89\u003c\/span\u003e \u003cspan class=\"upsell-size2\"\u003e500 g\u003c\/span\u003e\n\u003c\/div\u003e\n\u003ca href=\"https:\/\/ausamics.com.au\/products\/terrific-broth-tb-high-yield-bacterial-media-ausamics\" class=\"upsell-btn2\"\u003eAdd Terrific Broth →\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- COMPLEMENTARY: PHAGE \/ HIGH-DENSITY --\u003e\n\u003cp class=\"sec-title2\"\u003ePhage \u0026amp; high-density culture\u003c\/p\u003e\n\u003cdiv class=\"upsell-grid2\"\u003e\n\u003cdiv class=\"upsell-card2\"\u003e\n\u003cdiv class=\"upsell-cat2\"\u003eHigh-Density Media\u003c\/div\u003e\n\u003cdiv class=\"upsell-name2\"\u003e2×YT Broth\u003c\/div\u003e\n\u003cdiv class=\"upsell-code2\"\u003eAS-1398\u003c\/div\u003e\n\u003cdiv class=\"upsell-desc2\"\u003eDouble-strength yeast-tryptone broth for phage display, antibody library construction, and high-density pre-culture.\u003c\/div\u003e\n\u003cdiv class=\"upsell-price-row2\"\u003e\n\u003cspan class=\"upsell-price2\"\u003e$95\u003c\/span\u003e \u003cspan class=\"upsell-size2\"\u003e500 g\u003c\/span\u003e\n\u003c\/div\u003e\n\u003ca href=\"https:\/\/ausamics.com.au\/products\/2-x-yt-broth\" class=\"upsell-btn2\"\u003eAdd 2×YT Broth →\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"upsell-card2\"\u003e\n\u003cdiv class=\"upsell-cat2\"\u003eSolid Media\u003c\/div\u003e\n\u003cdiv class=\"upsell-name2\"\u003e2×YT Agar\u003c\/div\u003e\n\u003cdiv class=\"upsell-code2\"\u003eAS-1395\u003c\/div\u003e\n\u003cdiv class=\"upsell-desc2\"\u003eDouble-strength yeast-tryptone agar for high-efficiency plating of phage display libraries and high-density E. coli colonies.\u003c\/div\u003e\n\u003cdiv class=\"upsell-price-row2\"\u003e\n\u003cspan class=\"upsell-price2\"\u003e$95\u003c\/span\u003e \u003cspan class=\"upsell-size2\"\u003e500 g\u003c\/span\u003e\n\u003c\/div\u003e\n\u003ca href=\"https:\/\/ausamics.com.au\/products\/2-x-yt-agar\" class=\"upsell-btn2\"\u003eAdd 2×YT Agar →\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- COMPLEMENTARY: DOWNSTREAM DNA ANALYSIS --\u003e\n\u003cp class=\"sec-title2\"\u003eDownstream DNA analysis\u003c\/p\u003e\n\u003cdiv class=\"upsell-grid2\"\u003e\n\u003cdiv class=\"upsell-card2\"\u003e\n\u003cdiv class=\"upsell-cat2\"\u003eGel Electrophoresis\u003c\/div\u003e\n\u003cdiv class=\"upsell-name2\"\u003eAgarose — Mol. Bio. Grade\u003c\/div\u003e\n\u003cdiv class=\"upsell-code2\"\u003eAS-1015\u003c\/div\u003e\n\u003cdiv class=\"upsell-desc2\"\u003eFor agarose gel electrophoresis following colony PCR, restriction digest, or plasmid size analysis. Low EEO for sharp band resolution.\u003c\/div\u003e\n\u003cdiv class=\"upsell-price-row2\"\u003e\n\u003cspan class=\"upsell-price2\"\u003e$79\u003c\/span\u003e \u003cspan class=\"upsell-size2\"\u003e50 g\u003c\/span\u003e\n\u003c\/div\u003e\n\u003ca href=\"https:\/\/ausamics.com.au\/products\/agarose-powder-low-eeo-agarose-molecular-biology-grade\" class=\"upsell-btn2\"\u003eAdd Agarose →\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- COMPLEMENTARY: BETA-GAL ASSAYS --\u003e\n\u003cp class=\"sec-title2\"\u003eβ-galactosidase assays\u003c\/p\u003e\n\u003cdiv class=\"upsell-grid2\"\u003e\n\u003cdiv class=\"upsell-card2\"\u003e\n\u003cdiv class=\"upsell-cat2\"\u003eScreening Substrate\u003c\/div\u003e\n\u003cdiv class=\"upsell-name2\"\u003eONPG\u003c\/div\u003e\n\u003cdiv class=\"upsell-code2\"\u003eASE-904\u003c\/div\u003e\n\u003cdiv class=\"upsell-desc2\"\u003eo-Nitrophenyl-β-D-galactopyranoside — colorimetric substrate for quantitative β-galactosidase activity assays, used in lacZ reporter studies and Miller unit calculations.\u003c\/div\u003e\n\u003cdiv class=\"upsell-price-row2\"\u003e\n\u003cspan class=\"upsell-price2\"\u003e$29\u003c\/span\u003e \u003cspan class=\"upsell-size2\"\u003e1 g\u003c\/span\u003e\n\u003c\/div\u003e\n\u003ca href=\"https:\/\/ausamics.com.au\/products\/2-nitrophenyl-beta-d-galactopyranoside-onpg\" class=\"upsell-btn2\"\u003eAdd ONPG →\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- COMPLEMENTARY: CUSTOM MEDIA FORMULATION --\u003e\n\u003cp class=\"sec-title2\"\u003eCustom media formulation\u003c\/p\u003e\n\u003cdiv class=\"upsell-grid2\"\u003e\n\u003cdiv class=\"upsell-card2\"\u003e\n\u003cdiv class=\"upsell-cat2\"\u003eBase Component\u003c\/div\u003e\n\u003cdiv class=\"upsell-name2\"\u003eBacteriological Peptone\u003c\/div\u003e\n\u003cdiv class=\"upsell-code2\"\u003eAS-1011\u003c\/div\u003e\n\u003cdiv class=\"upsell-desc2\"\u003eFor laboratories preparing their own LB or other bacterial media from base components rather than pre-formulated dehydrated media.\u003c\/div\u003e\n\u003cdiv class=\"upsell-price-row2\"\u003e\n\u003cspan class=\"upsell-price2\"\u003e$29\u003c\/span\u003e \u003cspan class=\"upsell-size2\"\u003e100 g (500 g \/ 1 kg \/ 5 kg also available)\u003c\/span\u003e\n\u003c\/div\u003e\n\u003ca href=\"https:\/\/ausamics.com.au\/products\/bacteriological-peptone-powder\" class=\"upsell-btn2\"\u003eAdd Peptone →\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"upsell-card2\"\u003e\n\u003cdiv class=\"upsell-cat2\"\u003eBase Component\u003c\/div\u003e\n\u003cdiv class=\"upsell-name2\"\u003eYeast Extract\u003c\/div\u003e\n\u003cdiv class=\"upsell-code2\"\u003eAS-1010\u003c\/div\u003e\n\u003cdiv class=\"upsell-desc2\"\u003eB-vitamins and accessory nutrients for custom media preparation alongside peptone and agar.\u003c\/div\u003e\n\u003cdiv class=\"upsell-price-row2\"\u003e\n\u003cspan class=\"upsell-price2\"\u003e$25\u003c\/span\u003e \u003cspan class=\"upsell-size2\"\u003e100 g (500 g \/ 1 kg also available)\u003c\/span\u003e\n\u003c\/div\u003e\n\u003ca href=\"https:\/\/ausamics.com.au\/products\/yeast-extract-powder\" class=\"upsell-btn2\"\u003eAdd Yeast Extract →\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"upsell-card2\"\u003e\n\u003cdiv class=\"upsell-cat2\"\u003eBase Component\u003c\/div\u003e\n\u003cdiv class=\"upsell-name2\"\u003eBacteriological Agar (Agar-Agar)\u003c\/div\u003e\n\u003cdiv class=\"upsell-code2\"\u003eAS-1001\u003c\/div\u003e\n\u003cdiv class=\"upsell-desc2\"\u003eSolidifying agent for custom-formulated solid media, for laboratories that prepare plates from individual components.\u003c\/div\u003e\n\u003cdiv class=\"upsell-price-row2\"\u003e\n\u003cspan class=\"upsell-price2\"\u003e$29\u003c\/span\u003e \u003cspan class=\"upsell-size2\"\u003e100 g (500 g \/ 1 kg also available)\u003c\/span\u003e\n\u003c\/div\u003e\n\u003ca href=\"https:\/\/ausamics.com.au\/products\/bacteriological-agar\" class=\"upsell-btn2\"\u003eAdd Agar →\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- WHY AUSAMICS --\u003e\n\u003cp class=\"sec-title2\"\u003eWhy AuSaMicS E. coli cloning media?\u003c\/p\u003e\n\u003cdiv class=\"why-grid2\"\u003e\n\u003cdiv class=\"why-item2\"\u003e\n\u003cdiv class=\"why-icon2\"\u003eAU\u003c\/div\u003e\n\u003cdiv class=\"why-text2\"\u003e\n\u003cstrong\u003eAustralian-manufactured\u003c\/strong\u003e\u003cspan\u003eCulture media manufactured locally in Melbourne for faster supply and reduced reliance on imported finished media.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"why-item2\"\u003e\n\u003cdiv class=\"why-icon2\"\u003e▶\u003c\/div\u003e\n\u003cdiv class=\"why-text2\"\u003e\n\u003cstrong\u003eFast Australian dispatch\u003c\/strong\u003e\u003cspan\u003eManufactured in Melbourne and dispatched promptly following production and batch release.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"why-item2\"\u003e\n\u003cdiv class=\"why-icon2\"\u003e■\u003c\/div\u003e\n\u003cdiv class=\"why-text2\"\u003e\n\u003cstrong\u003eFull documentation\u003c\/strong\u003e\u003cspan\u003eSDS, TDS and batch-specific COA provided for each product in the set.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"why-item2\"\u003e\n\u003cdiv class=\"why-icon2\"\u003e●\u003c\/div\u003e\n\u003cdiv class=\"why-text2\"\u003e\n\u003cstrong\u003eLOT \u0026amp; EXP traceability\u003c\/strong\u003e\u003cspan\u003eBatch-coded to support laboratory traceability, documentation and audit requirements.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"why-item2\"\u003e\n\u003cdiv class=\"why-icon2\"\u003e4→1\u003c\/div\u003e\n\u003cdiv class=\"why-text2\"\u003e\n\u003cstrong\u003eOne order, coordinated set\u003c\/strong\u003e\u003cspan\u003eCore cloning media sourced together, with antibiotic and recovery medium configured to your protocol.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"why-item2\"\u003e\n\u003cdiv class=\"why-icon2\"\u003e◆\u003c\/div\u003e\n\u003cdiv class=\"why-text2\"\u003e\n\u003cstrong\u003eDirect formulator support\u003c\/strong\u003e\u003cspan\u003eSpeak directly with the chemist. Protocol guidance and custom quantities available on request.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- FAQ --\u003e\n\u003cp class=\"sec-title2\"\u003eFrequently asked questions\u003c\/p\u003e\n\u003cdiv style=\"margin-bottom: 28px;\"\u003e\n\u003cdiv class=\"faq-item2\"\u003e\n\u003cdiv class=\"faq-q2\"\u003eQ1: What is included in the E. coli Cloning \u0026amp; Blue-White Screening Media Set?\u003c\/div\u003e\n\u003cdiv class=\"faq-a2\"\u003eThe core set is LB Agar (Lennox) 500 g, LB Broth (Lennox) 500 g, IPTG 1 g, and X-Gal 1 g — the media and screening substrates common to most standard cloning and blue-white screening workflows. Your selection antibiotic and, if needed, SOC Medium are configured separately above and added as their own line items.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq-item2\"\u003e\n\u003cdiv class=\"faq-q2\"\u003eQ2: Which antibiotic should I choose?\u003c\/div\u003e\n\u003cdiv class=\"faq-a2\"\u003eSelection depends on the resistance marker carried by your vector or plasmid — not on the cloning workflow itself. Check your vector map or plasmid documentation and use the selection conditions specified by the applicable protocol.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq-item2\"\u003e\n\u003cdiv class=\"faq-q2\"\u003eQ3: Do I need SOC Medium?\u003c\/div\u003e\n\u003cdiv class=\"faq-a2\"\u003eSOC Medium is used for the post-transformation recovery step — the incubation period immediately after heat shock or electroporation, before plating. It's not required for every protocol; some labs use LB for recovery instead. Add it if your transformation protocol calls for a dedicated rich recovery medium.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq-item2\"\u003e\n\u003cdiv class=\"faq-q2\"\u003eQ4: What are IPTG and X-Gal used for?\u003c\/div\u003e\n\u003cdiv class=\"faq-a2\"\u003eIPTG is a non-metabolisable inducer of lac-controlled expression, while X-Gal is a chromogenic β-galactosidase substrate. Together they enable blue-white colony screening in a compatible host\/vector system with an appropriate lacZ α-complementation system. Confirm that your strain and vector support this before relying on colony colour for screening.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq-item2\"\u003e\n\u003cdiv class=\"faq-q2\"\u003eQ5: What's the difference between LB Lennox and LB Miller formulations?\u003c\/div\u003e\n\u003cdiv class=\"faq-a2\"\u003eLB Lennox contains 5 g\/L NaCl, while LB Miller contains 10 g\/L NaCl. Both support E. coli growth; Lennox is the formulation used in this set by default. Miller versions (AS-1267, AS-1270) are available individually if your protocol specifies it.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq-item2\"\u003e\n\u003cdiv class=\"faq-q2\"\u003eQ6: Can I order different pack sizes or quantities?\u003c\/div\u003e\n\u003cdiv class=\"faq-a2\"\u003eYes — all products in this set are available individually and in bulk pack sizes. For custom configurations or larger quantities, use the \"Configure this set for your laboratory\" link above or contact support@ausamics.com.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- FINAL CTA --\u003e\n\u003cdiv class=\"sal-product\" style=\"text-align:center;\"\u003e\n\u003cdiv class=\"sal-product-name\" style=\"margin-bottom:4px;\"\u003eE. coli Cloning \u0026amp; Blue-White Screening Media Set\u003c\/div\u003e\n\u003cdiv class=\"sal-product-sub\" style=\"margin:0 auto 18px;\"\u003eLB Agar · LB Broth · IPTG · X-Gal — plus your chosen antibiotic and optional SOC Medium\u003c\/div\u003e\n\u003ca href=\"#\" id=\"ecl-cta-bottom\" class=\"sal-btn-cta\"\u003eAdd Cloning Media Set — $287\u003c\/a\u003e\n\u003c\/div\u003e\n\n\u003c!-- DISCLAIMER --\u003e\n\u003cdiv class=\"disclaimer2\"\u003e\n\u003cstrong\u003eResearch Use Only.\u003c\/strong\u003e Products in this set are supplied for in vitro laboratory research by appropriately trained personnel. Selection antibiotics and screening reagents should be used according to the applicable vector, host-strain and institutional protocols — consult individual SDS and product documentation before use. Full terms and liability information: \u003ca href=\"https:\/\/ausamics.com.au\/policies\/terms-of-service\" style=\"color:#92400e;font-weight:700;\"\u003eausamics.com.au\/policies\/terms-of-service\u003c\/a\u003e.\u003c\/div\u003e\n\n\u003c!-- FOOTER --\u003e\n\u003cdiv class=\"sal-footer\"\u003e\n\u003cstrong\u003eAuSaMicS Pty Ltd\u003c\/strong\u003e | ABN 56 676 640 467\u003cbr\u003e36\/25 Trafalgar Road, Epping, VIC 3076, Australia\u003cbr\u003e\u003ca href=\"https:\/\/www.ausamics.com.au\"\u003ewww.ausamics.com.au\u003c\/a\u003e | \u003ca href=\"mailto:support@ausamics.com\"\u003esupport@ausamics.com\u003c\/a\u003e | +61 412 520 598\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- \/sal-wrap --\u003e\n\n\u003c!-- ============================================================\n     CART INTEGRATION (Shopify AJAX Cart API)\n     ============================================================\n     REQUIRED BEFORE THIS GOES LIVE — all IDs below are placeholders:\n     1. Create \"E. coli Cloning \u0026 Blue-White Screening Media Set\"\n        (the 4-item core set) as an actual $260 product in Shopify\n        Admin, then replace CORE_VARIANT_ID with its real variant ID.\n     2. Ampicillin, Kanamycin and Chloramphenicol need standalone\n        product pages\/variant IDs if they don't already have one —\n        replace AMPICILLIN_VARIANT_ID \/ KANAMYCIN_VARIANT_ID \/\n        CHLORAMPHENICOL_VARIANT_ID accordingly.\n     3. SOC_VARIANT_ID below IS real, pulled from the live SOC\n        Medium product page.\n     Send me the live product\/variant URLs once created (same as we\n     did for the Salmonella set) and I'll fill these in for you.\n     ============================================================ --\u003e\n\u003cscript\u003e\n(function () {\n  var CORE_VARIANT_ID = 48411624702207; \/\/ E. coli Cloning \u0026 Blue-White Screening Media Set (real)\n  var AMPICILLIN_VARIANT_ID = 48051910050047; \/\/ Ampicillin Sodium Salt 1 g (real)\n  var KANAMYCIN_VARIANT_ID = 48126406885631; \/\/ Kanamycin Sulfate 1 g (real)\n  var CHLORAMPHENICOL_VARIANT_ID = 48100274176255; \/\/ Chloramphenicol 5 g (real)\n  var SOC_VARIANT_ID = 48411276411135; \/\/ SOC Medium 500 g, $85 (real, confirmed)\n\n  var CORE_PRICE = 260;\n\n  function selectedAntibiotic() {\n    var radios = document.getElementsByName('ecl-antibiotic');\n    for (var i = 0; i \u003c radios.length; i++) {\n      if (radios[i].checked) return radios[i];\n    }\n    return null;\n  }\n\n  function antibioticVariantId(radio) {\n    if (radio.id === 'ecl-ab-amp') return AMPICILLIN_VARIANT_ID;\n    if (radio.id === 'ecl-ab-kan') return KANAMYCIN_VARIANT_ID;\n    if (radio.id === 'ecl-ab-cam') return CHLORAMPHENICOL_VARIANT_ID;\n    return null; \/\/ 'no antibiotic required' — nothing to add\n  }\n\n  function updateTotal() {\n    var radio = selectedAntibiotic();\n    var abPrice = radio ? parseInt(radio.value, 10) : 0;\n    var socChecked = document.getElementById('ecl-soc-checkbox').checked;\n    var total = CORE_PRICE + abPrice + (socChecked ? 85 : 0);\n\n    var priceEl = document.getElementById('ecl-total-price');\n    if (priceEl) priceEl.textContent = '$' + total;\n\n    var label = 'Add Cloning Media Set — $' + total;\n    var top = document.getElementById('ecl-cta-top');\n    var bottom = document.getElementById('ecl-cta-bottom');\n    if (top) top.textContent = label;\n    if (bottom) bottom.textContent = label;\n  }\n\n  function buildItems() {\n    var items = [{ id: CORE_VARIANT_ID, quantity: 1 }];\n    var radio = selectedAntibiotic();\n    if (radio) {\n      var abId = antibioticVariantId(radio);\n      if (abId) items.push({ id: abId, quantity: 1 });\n    }\n    if (document.getElementById('ecl-soc-checkbox').checked) {\n      items.push({ id: SOC_VARIANT_ID, quantity: 1 });\n    }\n    return items;\n  }\n\n  function hasPlaceholder(items) {\n    return items.some(function (item) {\n      return typeof item.id === 'string' \u0026\u0026 item.id.indexOf('REPLACE_WITH') === 0;\n    });\n  }\n\n  function addToCart(button) {\n    var items = buildItems();\n    if (hasPlaceholder(items)) {\n      alert('Set up required: one or more products in this configuration still need a real variant ID before this button will work.');\n      return;\n    }\n    var originalText = button.textContent;\n    button.textContent = 'Adding…';\n    button.style.pointerEvents = 'none';\n\n    fetch('\/cart\/add.js', {\n      method: 'POST',\n      headers: { 'Content-Type': 'application\/json', 'Accept': 'application\/json' },\n      body: JSON.stringify({ items: items })\n    })\n      .then(function (res) {\n        if (!res.ok) return res.json().then(function (err) { throw err; });\n        return res.json();\n      })\n      .then(function () {\n        window.location.href = '\/cart';\n      })\n      .catch(function (err) {\n        console.error('Add to cart failed:', err);\n        alert('Sorry — could not add to cart. Please try again or contact support@ausamics.com.');\n        button.textContent = originalText;\n        button.style.pointerEvents = 'auto';\n      });\n  }\n\n  document.addEventListener('DOMContentLoaded', function () {\n    var top = document.getElementById('ecl-cta-top');\n    var bottom = document.getElementById('ecl-cta-bottom');\n    var soc = document.getElementById('ecl-soc-checkbox');\n    var radios = document.getElementsByName('ecl-antibiotic');\n\n    if (top) top.addEventListener('click', function (e) { e.preventDefault(); addToCart(top); });\n    if (bottom) bottom.addEventListener('click', function (e) { e.preventDefault(); addToCart(bottom); });\n    if (soc) soc.addEventListener('change', updateTotal);\n    for (var i = 0; i \u003c radios.length; i++) radios[i].addEventListener('change', updateTotal);\n\n    updateTotal();\n  });\n})();\n\u003c\/script\u003e","brand":"Ausamics Life Science","offers":[{"title":"Default Title","offer_id":48411624702207,"sku":null,"price":260.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/E.coliMolecularBiologyBundles_AustralianMade_b7089b22-338c-4822-9bce-9363a10c0e87.jpg?v=1776679838","url":"https:\/\/ausamics.com.au\/products\/e-coli-cloning-blue-white-screening-media-set","provider":"AuSaMicS","version":"1.0","type":"link"}