{"product_id":"ec-medium-modified-mec-broth","title":"mEC Broth | Modified EC Broth with Novobiocin | AS-1213 | AuSaMicS","description":"\u003cdiv class=\"aus-mec-product\" style=\"font-family: 'Segoe UI', system-ui, -apple-system, sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\"\u003e\n\u003c!-- Hero Section --\u003e\n\u003cdiv class=\"aus-hero-section\" style=\"background: linear-gradient(135deg, #f0f4f8 0%, #d9e2ec 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #d8e1e8; border-left: 8px solid #123d6b;\"\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\" class=\"aus-hero-grid\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #1d6fb8; font-weight: bold; margin-bottom: 12px; background: #fff; padding: 6px 12px; border-radius: 20px; border: 1px solid #d8e1e8;\"\u003eAuSaMicS Life Science • Selective Enrichment\u003c\/div\u003e\n\u003ch1 style=\"color: #15324b; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003emEC Broth\u003c\/h1\u003e\n\u003ch2 style=\"color: #243b53; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: 0.9;\"\u003eModified EC Broth with Novobiocin\u003c\/h2\u003e\n\u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #334e68; max-width: 600px; font-weight: 500;\"\u003ePremium selective enrichment medium for \u003cstrong\u003eE. coli O157:H7 detection\u003c\/strong\u003e. Enhanced with Novobiocin for \u0026gt;95% suppression of Gram-positive interference. Gold standard for food safety and pathogen surveillance programs.\u003c\/p\u003e\n\u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n\u003cspan style=\"background: #123d6b; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAS-1213\u003c\/span\u003e \u003cspan style=\"background: #fff; border: 2px solid #dc3545; color: #dc3545; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eO157:H7\u003c\/span\u003e \u003cspan style=\"background: #28a745; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e✓ Australian Stock\u003c\/span\u003e \u003cspan style=\"background: #ef6c00; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e⚠️ Selective\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #4a5568;\"\u003e\n\u003cspan\u003e\u003cstrong\u003eSame-Day Dispatch:\u003c\/strong\u003e Australian Stock\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eUSDA-FSIS Grade:\u003c\/strong\u003e Validated protocol\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003ePathogen Enrichment:\u003c\/strong\u003e BSL-2 conditions\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Quality Guarantee --\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 280px; border: 1px solid #d8e1e8;\" class=\"aus-guarantee\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003e O157:H7 Specialist\u003c\/h4\u003e\n\u003cdiv style=\"font-size: 14px; color: #4a5568; text-align: center; line-height: 1.5;\"\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003ePathogen Expert\u003c\/strong\u003e - EHEC enrichment\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eFood Safety\u003c\/strong\u003e - Meat \u0026amp; dairy testing\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eComplete Package\u003c\/strong\u003e - COA + BSL protocols\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eFast Supply\u003c\/strong\u003e - Ships within 24h\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eExpert Support\u003c\/strong\u003e - Pathogen method validation\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-top: 20px; padding: 15px; background: #fff8e1; border-radius: 12px; text-align: center; border: 1px solid #ef6c00;\"\u003e\n\u003cdiv style=\"color: #ef6c00; font-weight: 600; font-size: 14px;\"\u003eNovobiocin Enhanced\u003c\/div\u003e\n\u003cdiv style=\"color: #4a5568; font-size: 13px; margin-top: 5px;\"\u003e95% Gram-positive suppression\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Product Details Grid --\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\" class=\"aus-main-grid\"\u003e\n\u003cdiv\u003e\n\u003c!-- Complete Formulation --\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003e Complete Formulation \u0026amp; Selectivity Mechanism\u003c\/h3\u003e\n\u003c!-- Detailed Ingredient Table --\u003e\n\u003cdiv style=\"background: #f8fafc; padding: 20px; border-radius: 16px; border-left: 5px solid #1d6fb8; margin-bottom: 20px;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px;\"\u003e📋 Ingredient Composition (per Liter)\u003c\/h4\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #d8e1e8; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n\u003cthead style=\"background: #f6f8fb;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eIngredient\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eConcentration\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003ePrimary Function\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eMechanism\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003ePeptone\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003e20.0 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eNitrogen source\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eCasein hydrolysate, amino acids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eLactose\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003e5.0 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #1565c0; font-weight: 600;\"\u003eFermentation substrate\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eGas production, E. coli detection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eSodium Chloride\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003e5.0 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eOsmotic balance\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eMaintains cell integrity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eBile Salts No. 3\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003e1.12 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #dc3545; font-weight: 600;\"\u003eSelective agent\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eInhibits Gram-positive bacteria\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003ePhosphates (Buffered)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003e5.50 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #17a2b8; font-weight: 600;\"\u003epH stabilization\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003ePrevents acid-induced cell death\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background: #fff8e1; border-bottom: 1px solid #ef6c00;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: bold; color: #ef6c00;\"\u003eNovobiocin\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #ef6c00; font-weight: bold;\"\u003e20 mg\/L\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #ef6c00; font-weight: bold;\"\u003eEnhanced selectivity\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #5d4037;\"\u003eDNA gyrase inhibition, Gram-positive suppression\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Selectivity Mechanism --\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d8e1e8;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px;\"\u003e Triple Selectivity Enhancement\u003c\/h4\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(200px, 1fr)); gap: 15px;\"\u003e\n\u003cdiv style=\"background: #ffebee; padding: 15px; border-radius: 12px; border-left: 4px solid #dc3545;\"\u003e\n\u003cstrong style=\"color: #c62828;\"\u003e1. Bile Salt Selection\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eDisrupts Gram-positive cell membranes\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff8e1; padding: 15px; border-radius: 12px; border-left: 4px solid #ef6c00;\"\u003e\n\u003cstrong style=\"color: #ef6c00;\"\u003e2. Novobiocin Enhancement\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eDNA gyrase inhibition, \u0026gt;95% suppression\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #e8f2ff; padding: 15px; border-radius: 12px; border-left: 4px solid #1565c0;\"\u003e\n\u003cstrong style=\"color: #1565c0;\"\u003e3. Lactose Fermentation\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eGas production confirms E. coli\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff8e1; padding: 15px; border-radius: 10px; margin-top: 15px; border: 1px solid #ef6c00;\"\u003e\n\u003cstrong style=\"color: #ef6c00;\"\u003eCritical Enhancement:\u003c\/strong\u003e Novobiocin at 20 mg\/L provides superior selectivity for O157:H7 isolation from complex food matrices with heavy Gram-positive contamination\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Comprehensive E. coli O157:H7 Enrichment Media Comparison --\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003e📊 Complete \u003cem\u003eE. coli \u003c\/em\u003eO157:H7 Enrichment Portfolio\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #d8e1e8; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05); min-width: 1000px;\"\u003e\n\u003cthead style=\"background: #f6f8fb;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eMedium\u003c\/th\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eSelectivity Level\u003c\/th\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eO157:H7 Recovery\u003c\/th\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eBackground Suppression\u003c\/th\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eKey Advantage\u003c\/th\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eStandards\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #fff8e1; border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; color: #ef6c00;\"\u003e\u003ca href=\"\/products\/mec-broth\" style=\"color: #ef6c00; text-decoration: none;\"\u003emEC Broth (Novobiocin) (AS-1213)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #28a745; font-weight: 600;\"\u003e⭐⭐⭐⭐⭐ Excellent\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #28a745; font-weight: 600;\"\u003e92-97%\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #28a745; font-weight: 600;\"\u003e95% Gram-positive\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eSuperior meat \u0026amp; dairy performance\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eUSDA-FSIS, ISO 16654\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003e\u003ca href=\"\/products\/ec-broth\" style=\"color: #1565c0; text-decoration: none; font-weight: 600;\"\u003eStandard EC Broth (AS-1214)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #17a2b8;\"\u003e⭐⭐⭐ Moderate\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #17a2b8;\"\u003e78-88%\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #ffc107;\"\u003e70% Gram-positive\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eWater quality, general coliforms\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eAPHA Standard Methods\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003e\u003ca href=\"\/products\/tsa-broth-vancomycin\" style=\"color: #6f42c1; text-decoration: none; font-weight: 600;\"\u003eTSA Broth (AS-1371) + Vancomycin (ASA-2025)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #28a745; font-weight: 600;\"\u003e⭐⭐⭐⭐ High\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #17a2b8;\"\u003e85-92%\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #17a2b8;\"\u003e85% Gram-positive\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eAlternative selective enrichment\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eResearch methods\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003e\u003ca href=\"\/products\/gram-negative-broth\" style=\"color: #dc3545; text-decoration: none; font-weight: 600;\"\u003eGN Broth (AS-1244)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #17a2b8;\"\u003e⭐⭐⭐ Moderate\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #ffc107;\"\u003e70-82%\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #17a2b8;\"\u003e80% Gram-positive\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eBroad Gram-negative enrichment\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eClinical microbiology\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003e\u003ca href=\"\/products\/lauryl-tryptose-broth\" style=\"color: #28a745; text-decoration: none; font-weight: 600;\"\u003eLauryl Tryptose Broth (AS-1266)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #ffc107;\"\u003e⭐⭐ Low\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #ffc107;\"\u003e65-78%\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #dc3545;\"\u003e40% Gram-positive\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eGentle on stressed cells\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003ePresumptive coliform\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px;\"\u003e\u003ca href=\"\/products\/ee-broth\" style=\"color: #fd7e14; text-decoration: none; font-weight: 600;\"\u003eEE Broth (AS-1216)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #17a2b8;\"\u003e⭐⭐⭐ Moderate\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #17a2b8;\"\u003e75-85%\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #ffc107;\"\u003e60% Gram-positive\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eBroad Enterobacteriaceae\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eEuropean methods\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Comprehensive Pros \u0026 Cons Analysis --\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003e Strategic Method Selection for O157:H7 Detection\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(300px, 1fr)); gap: 20px; margin-bottom: 25px;\"\u003e\n\u003c!-- mEC Broth Advantages --\u003e\n\u003cdiv style=\"background: #e8f5e8; padding: 20px; border-radius: 16px; border: 1px solid #c8e6c9;\"\u003e\n\u003ch4 style=\"color: #2e7d32; margin: 0 0 15px 0; font-size: 16px;\"\u003e✅ mEC Broth Advantages\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003eHighest Recovery:\u003c\/strong\u003e 92-97% for O157:H7\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003eSuperior Selectivity:\u003c\/strong\u003e \u0026gt;95% Gram-positive suppression\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003eFood Matrix Optimized:\u003c\/strong\u003e Meat \u0026amp; dairy products\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003eRegulatory Validated:\u003c\/strong\u003e USDA-FSIS approved\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003epH Buffered:\u003c\/strong\u003e Prevents acid-induced death\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003ePathogen Focus:\u003c\/strong\u003e EHEC detection optimized\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- mEC Broth Considerations --\u003e\n\u003cdiv style=\"background: #fff3e0; padding: 20px; border-radius: 16px; border: 1px solid #ffcc02;\"\u003e\n\u003ch4 style=\"color: #e65100; margin: 0 0 15px 0; font-size: 16px;\"\u003e⚠️ mEC Considerations\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #e65100;\"\u003e▲ \u003cstrong\u003eBSL-2 Required:\u003c\/strong\u003e Pathogen enrichment protocols\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #e65100;\"\u003e▲ \u003cstrong\u003eNovobiocin Critical:\u003c\/strong\u003e Lot-specific QC needed\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #e65100;\"\u003e▲ \u003cstrong\u003eConfirmation Required:\u003c\/strong\u003e SMAC plating necessary\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #e65100;\"\u003e▲ \u003cstrong\u003eHigher Cost:\u003c\/strong\u003e vs. standard EC broth\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #e65100;\"\u003e▲ \u003cstrong\u003eStorage Sensitive:\u003c\/strong\u003e Prepared medium 2 weeks max\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- Application-Specific Selection Guide --\u003e\n\u003cdiv style=\"background: #ffebee; padding: 20px; border-radius: 16px; border: 1px solid #ef5350;\"\u003e\n\u003ch4 style=\"color: #c62828; margin: 0 0 15px 0; font-size: 16px;\"\u003e🎯 Application-Specific Selection\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #c62828;\"\u003e◆ \u003cstrong\u003eGround Beef:\u003c\/strong\u003e mEC Broth (heavy Gram-positive flora)\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #c62828;\"\u003e◆ \u003cstrong\u003eRaw Milk:\u003c\/strong\u003e mEC Broth (enterococci interference)\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #c62828;\"\u003e◆ \u003cstrong\u003eWater Quality:\u003c\/strong\u003e Standard EC (general coliforms)\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #c62828;\"\u003e◆ \u003cstrong\u003eProduce:\u003c\/strong\u003e TSA+Vancomycin (alternative method)\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #c62828;\"\u003e◆ \u003cstrong\u003eClinical Isolates:\u003c\/strong\u003e GN Broth (broad spectrum)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Sample Type Performance Matrix --\u003e\n\u003cdiv style=\"background: #f8fafc; padding: 20px; border-radius: 16px; border-left: 5px solid #dc3545;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px;\"\u003e Sample-Specific O157:H7 Recovery Rates\u003c\/h4\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #d8e1e8; border-radius: 8px; overflow: hidden;\"\u003e\n\u003cthead style=\"background: #dc3545; color: #fff;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 12px; border-bottom: 1px solid #d8e1e8; text-align: left;\"\u003eSample Matrix\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border-bottom: 1px solid #d8e1e8; text-align: left;\"\u003emEC Broth\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border-bottom: 1px solid #d8e1e8; text-align: left;\"\u003eStandard EC\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border-bottom: 1px solid #d8e1e8; text-align: left;\"\u003eKey Challenge\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody style=\"background: #fff;\"\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eGround Beef (80\/20)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #28a745; font-weight: 600;\"\u003e92-97%\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #ffc107;\"\u003e65-78%\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eHigh Gram-positive background\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eRaw Milk\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #28a745; font-weight: 600;\"\u003e88-94%\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #ffc107;\"\u003e58-72%\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eEnterococci interference\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eFresh Produce\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #28a745; font-weight: 600;\"\u003e85-92%\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #17a2b8;\"\u003e75-85%\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eEnvironmental bacteria\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003ePoultry (Carcass rinse)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #28a745; font-weight: 600;\"\u003e90-95%\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #ffc107;\"\u003e68-80%\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eMultiple pathogen types\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eCheese (Soft)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #28a745; font-weight: 600;\"\u003e87-93%\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #ffc107;\"\u003e55-68%\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eLactic acid bacteria\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eEnvironmental Samples\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #17a2b8;\"\u003e82-88%\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #17a2b8;\"\u003e75-82%\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eDiverse microbial community\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Protocol \u0026 Quality Control --\u003e\n\u003cdiv style=\"background: linear-gradient(135deg, #ffebee 0%, #f8bbd9 100%); padding: 20px; border-radius: 16px; border-left: 5px solid #dc3545;\"\u003e\n\u003ch4 style=\"color: #c62828; margin: 0 0 15px 0; font-size: 16px;\"\u003e🔬 O157:H7 Detection Protocol \u0026amp; Quality Control\u003c\/h4\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(200px, 1fr)); gap: 15px;\"\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #c62828;\"\u003eEnrichment Protocol:\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003e25g sample + 225mL mEC\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003e35-37°C, 24-48 hours\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #c62828;\"\u003ePositive Control:\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003e\u003cem\u003eE. coli\u003c\/em\u003e O157:H7 ATCC 43888\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eGas production + turbidity\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #c62828;\"\u003eNegative Control:\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003e\u003cem\u003eEnterococcus faecalis\u003c\/em\u003e\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eNo growth (suppressed)\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #c62828;\"\u003eConfirmation:\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eSMAC agar plating\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eSorbitol-negative colonies\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: rgba(255,255,255,0.7); padding: 15px; border-radius: 10px; margin-top: 15px; border: 1px solid #ef5350;\"\u003e\n\u003cstrong style=\"color: #c62828;\"\u003e⚠️ BSL-2 Safety Requirements:\u003c\/strong\u003e Handle under appropriate biosafety conditions. This medium enriches for human pathogens. Use universal precautions and autoclave all waste.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Right Column --\u003e\n\u003cdiv\u003e\n\u003c!-- Technical Specifications --\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #d8e1e8; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003e📋 Technical Specifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; width: 45%; background: #f6f8fb;\"\u003eCatalogue Number\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eAS-1213\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eDehydrated Weight\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e36.62 g\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eFinal pH\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e6.9 ± 0.2 (at 25°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eNovobiocin Level\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #ef6c00; font-weight: 600;\"\u003e20 mg\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eAppearance (Powder)\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eLight cream to beige\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003ePrepared Medium\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eLight amber, clear broth\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eSterilization\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #d9534f; font-weight: 600;\"\u003e121°C, 15 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eIncubation\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e35-37°C, 24-48 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eShelf Life\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #28a745; font-weight: 600;\"\u003e3 years\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c!-- Target Organisms --\u003e\n\u003cdiv style=\"background: #ffebee; padding: 20px; border-radius: 16px; border: 1px solid #ef5350; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #c62828; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e Target Pathogens\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #c62828;\"\u003e\n\u003cstrong\u003e\u003cem\u003eE. coli\u003c\/em\u003e O157:H7\u003c\/strong\u003e - Primary EHEC strain\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #c62828;\"\u003e\n\u003cstrong\u003e\u003cem\u003eE. coli\u003c\/em\u003e O26\u003c\/strong\u003e - Non-O157 STEC\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #c62828;\"\u003e\n\u003cstrong\u003e\u003cem\u003eE. coli\u003c\/em\u003e O103\u003c\/strong\u003e - Emerging strain\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #c62828;\"\u003e\n\u003cstrong\u003e\u003cem\u003eE. coli\u003c\/em\u003e O111\u003c\/strong\u003e - Clinical significance\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #c62828;\"\u003e\n\u003cstrong\u003eFecal Coliforms\u003c\/strong\u003e - General detection\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- Regulatory Standards --\u003e\n\u003cdiv style=\"background: #e8f2ff; padding: 20px; border-radius: 16px; border: 1px solid #90caf9; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #1565c0; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e📜 Regulatory Compliance\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eUSDA-FSIS MLG 5C\u003c\/strong\u003e - O157:H7 detection\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eISO 16654:2001\u003c\/strong\u003e - EHEC detection\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eFDA BAM Chapter 4A\u003c\/strong\u003e - E. coli methods\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eHealth Canada MFHPB-30\u003c\/strong\u003e - O157 methods\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eAOAC Official Method 995.14\u003c\/strong\u003e - Validated\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- Critical Success Factors --\u003e\n\u003cdiv style=\"background: #fff8e1; padding: 20px; border-radius: 16px; border: 1px solid #ef6c00; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #ef6c00; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e⚠️ Critical Success Factors\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #ef6c00;\"\u003e\n\u003cstrong\u003eTemperature:\u003c\/strong\u003e 35-37°C ± 1°C precision\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #ef6c00;\"\u003e\n\u003cstrong\u003eTiming:\u003c\/strong\u003e Check at 18-24h and 48h\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #ef6c00;\"\u003e\n\u003cstrong\u003eDurham Tubes:\u003c\/strong\u003e Inverted for gas detection\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #ef6c00;\"\u003e\n\u003cstrong\u003eNovobiocin QC:\u003c\/strong\u003e Lot-specific validation\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #ef6c00;\"\u003e\n\u003cstrong\u003eBSL-2:\u003c\/strong\u003e Pathogen safety protocols\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- Quality Assurance --\u003e\n\u003cdiv style=\"background: #e8f5e8; padding: 20px; border-radius: 16px; border: 1px solid #c8e6c9; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #2e7d32; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e✅ Quality Standards\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003ePerformance Testing:\u003c\/strong\u003e O157:H7 validation\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003eSelectivity Testing:\u003c\/strong\u003e Gram-positive suppression\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003epH Verification:\u003c\/strong\u003e 6.9 ± 0.2 confirmed\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003eSterility Testing:\u003c\/strong\u003e USP \u0026lt;71\u0026gt; compliant\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003eBSL Documentation:\u003c\/strong\u003e Safety protocols included\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- Documentation --\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Related Products --\u003e\n\u003cdiv style=\"background: #f8fafc; padding: 30px; border-radius: 18px; border: 1px solid #d8e1e8; margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 20px 0; text-align: center; font-size: 20px;\"\u003e Complete O157:H7 Detection System\u003c\/h3\u003e\n\u003c!-- Enrichment \u0026 Supporting Media --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003eEnrichment \u0026amp; Supporting Media\u003c\/h4\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(180px, 1fr)); gap: 15px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #1565c0; margin: 0 0 8px 0; font-size: 14px;\"\u003eStandard EC Broth (AS-1214)\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eGeneral coliform enrichment\u003c\/p\u003e\n\u003ca href=\"\/products\/ec-medium-broth\" style=\"display: inline-block; background: #1565c0; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #28a745; margin: 0 0 8px 0; font-size: 14px;\"\u003eLauryl Tryptose Broth (AS-1266)\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003ePresumptive coliform\u003c\/p\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"display: inline-block; background: #28a745; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #6f42c1; margin: 0 0 8px 0; font-size: 14px;\"\u003eTSA (AS-1370) + Vancomycin \u003ca href=\"\/products\/tsa-broth-vancomycin\" style=\"color: #6f42c1; text-decoration: none; font-weight: 600;\"\u003e(ASA-2025)\u003c\/a\u003e\n\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eAlternative selective broth\u003c\/p\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"display: inline-block; background: #6f42c1; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #fd7e14; margin: 0 0 8px 0; font-size: 14px;\"\u003eEE Broth (AS-1216)\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eEnterobacteriaceae enrichment\u003c\/p\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"display: inline-block; background: #fd7e14; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Selective Plating Media --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003eSelective Plating Media\u003c\/h4\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(180px, 1fr)); gap: 15px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #dc3545; margin: 0 0 8px 0; font-size: 14px;\"\u003eSMAC Agar\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eSorbitol MacConkey\u003c\/p\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"display: inline-block; background: #dc3545; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #e91e63; margin: 0 0 8px 0; font-size: 14px;\"\u003eCT-SMAC Agar\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eCefixime-tellurite enhanced\u003c\/p\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"display: inline-block; background: #e91e63; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #9c27b0; margin: 0 0 8px 0; font-size: 14px;\"\u003eRainbow Agar O157\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eChromogenic detection\u003c\/p\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"display: inline-block; background: #9c27b0; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #795548; margin: 0 0 8px 0; font-size: 14px;\"\u003eMacConkey Agar\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eGeneral selective plating\u003c\/p\u003e\n\u003ca href=\"\/products\/macconkey-agar\" style=\"display: inline-block; background: #795548; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Confirmation \u0026 Identification --\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003eConfirmation \u0026amp; Identification\u003c\/h4\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(180px, 1fr)); gap: 15px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #c62828; margin: 0 0 8px 0; font-size: 14px;\"\u003eO157 Latex Agglutination\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eRapid serotype confirmation\u003c\/p\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"display: inline-block; background: #c62828; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #1976d2; margin: 0 0 8px 0; font-size: 14px;\"\u003eSTEC Toxin Detection\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eShiga toxin immunoassay\u003c\/p\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"display: inline-block; background: #1976d2; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #388e3c; margin: 0 0 8px 0; font-size: 14px;\"\u003eSorbitol Solution\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eBiochemical testing\u003c\/p\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"display: inline-block; background: #388e3c; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #f57c00; margin: 0 0 8px 0; font-size: 14px;\"\u003eAPI 20E Strips\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eSpecies identification\u003c\/p\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"display: inline-block; background: #f57c00; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Collection Links --\u003e\n\u003cdiv style=\"text-align: center; margin-top: 25px;\"\u003e\n\u003cdiv style=\"display: flex; justify-content: center; gap: 15px; flex-wrap: wrap;\"\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"color: #dc3545; font-weight: 600; text-decoration: none; border: 2px solid #dc3545; padding: 10px 20px; border-radius: 8px;\"\u003e O157:H7 Detection\u003c\/a\u003e \u003ca href=\"\/collections\/culture-media\" style=\"color: #1d6fb8; font-weight: 600; text-decoration: none; border: 2px solid #1d6fb8; padding: 10px 20px; border-radius: 8px;\"\u003e Enrichment Media\u003c\/a\u003e \u003ca href=\"\/pages\/request-a-quote\" style=\"color: #ef6c00; font-weight: 600; text-decoration: none; border: 2px solid #ef6c00; padding: 10px 20px; border-radius: 8px;\"\u003e Pathogen Detection\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Footer --\u003e\n\u003cdiv style=\"text-align: center; padding: 30px 0; border-top: 1px solid #d8e1e8;\"\u003e\n\u003cdiv style=\"margin-bottom: 20px;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 10px 0; font-size: 16px;\"\u003eNeed O157:H7 Detection Support?\u003c\/h4\u003e\n\u003cp style=\"margin: 0; color: #6c757d; font-size: 14px;\"\u003eOur pathogen specialists can help optimize your EHEC detection and BSL-2 protocols\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; justify-content: center; gap: 20px; flex-wrap: wrap; margin-bottom: 25px;\"\u003e\n\u003ca href=\"tel:+61412520598\" style=\"display: flex; align-items: center; gap: 8px; color: #1d6fb8; text-decoration: none; font-weight: 600; padding: 12px 20px; border: 1px solid #1d6fb8; border-radius: 10px;\"\u003e📞 +61 412 520 598\u003c\/a\u003e \u003ca href=\"mailto:support@ausamics.com.au\" style=\"display: flex; align-items: center; gap: 8px; color: #1d6fb8; text-decoration: none; font-weight: 600; padding: 12px 20px; border: 1px solid #1d6fb8; border-radius: 10px;\"\u003e📧 Pathogen Support\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cp style=\"margin: 0; font-size: 12px; color: #94a3b8; line-height: 1.4;\"\u003e\u003cstrong\u003e⚠️ For pathogen detection and laboratory use only.\u003c\/strong\u003e BSL-2 protocols required. Not for food, feed, household, cosmetic, therapeutic, or personal use.\u003cbr\u003eAuSaMicS Pty Ltd • ABN: 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia\u003cbr\u003eSame-day dispatch • Australian stock • USDA-FSIS compliance documentation included\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Enhanced Mobile Responsiveness --\u003e\n\u003cstyle\u003e\n@media (max-width: 860px) {\n  .aus-hero-grid, .aus-main-grid { \n    grid-template-columns: 1fr !important; \n  }\n  .aus-guarantee { \n    min-width: 100% !important; \n    margin-top: 20px; \n  }\n  .aus-mec-product {\n    padding: 15px !important;\n  }\n  .aus-mec-product h1 {\n    font-size: 24px !important;\n  }\n  .aus-mec-product h2 {\n    font-size: 18px !important;\n  }\n  .aus-mec-product table {\n    font-size: 12px !important;\n  }\n}\n\n@media (max-width: 640px) {\n  .aus-hero-section {\n    padding: 25px !important;\n  }\n  .aus-main-grid \u003e div:first-child \u003e div {\n    margin-bottom: 25px !important;\n  }\n  .aus-mec-product table th,\n  .aus-mec-product table td {\n    padding: 8px 6px !important;\n  }\n}\n\u003c\/style\u003e","brand":"Ausamics Life Science","offers":[{"title":"100 g","offer_id":48373937701119,"sku":"AS-1213-100G","price":49.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":48373937733887,"sku":"AS-1213-500G","price":129.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/mEC_Broth_Modified_EC_Broth.jpg?v=1775525314","url":"https:\/\/ausamics.com.au\/products\/ec-medium-modified-mec-broth","provider":"AuSaMicS","version":"1.0","type":"link"}