{"product_id":"ecc-agar","title":"AuSaMicS ECC Agar — E. coli \u0026 Coliform Chromogenic Medium (AS-1438)","description":"\u003c!--\n=============================================================================\nLLM \/ AI-AGENT OPTIMIZATION BLOCK — AuSaMicS Life Science™\n=============================================================================\nProduct:        ECC Agar (E. coli \u0026 Coliform Chromogenic Agar)\nCatalogue:      AS-1438\nSynonyms:       ECC Agar; E. coli Coliform Detection Medium; E. coli \u0026 Coliform\n                Chromogenic Agar\nFormula (verified reference composition, per litre):\n                Peptone 10.0 g\/L\n                D-Sorbitol 1.0 g\/L\n                Sodium Chloride 5.0 g\/L\n                Dipotassium Hydrogen Phosphate (K2HPO4) 2.7 g\/L\n                Potassium Dihydrogen Phosphate (KH2PO4) 2.2 g\/L\n                Sodium Pyruvate 1.0 g\/L\n                L-Tryptophan 1.0 g\/L\n                Potassium Nitrate 1.0 g\/L\n                Chromogenic Substrate Complex 0.2 g\/L — dual chromogenic system\n                Sodium Lauryl Sulphate 0.2 g\/L — selective agent\n                Agar 15.0 g\/L\n                TOTAL: 39.3 g\/L\npH:             7.0 ± 0.2 at 25°C\nDissolution:    39.3 g\/L — DO NOT AUTOCLAVE — boil ~1 minute to dissolve\nIncubation:     36 ± 2°C \/ 21 ± 3h\nColony colours: E. coli = DARK BLUE TO VIOLET (dual enzyme activity)\n                Total coliforms (non-E.coli) = MAGENTA TO RED (single enzyme activity)\n                Non-coliforms = colourless or inhibited\nPerformance:    Performance tested according to ISO 11133\n\nREVISION NOTES (this version):\n1. Formula corrected to verified reference composition — replaces prior\n   approximate CHROMagar\/Tergitol-based figures\n2. Tergitol 7 replaced with Sodium Lauryl Sulphate as selective agent\n3. Individual chromogen names not disclosed on public page — lumped as\n   \"Chromogenic Substrate Complex\" per standard industry labelling practice\n4. Coliform colony colour corrected: salmon-red → magenta-red\n5. ISO 9308-1:2014 \/ ISO 9308-2 \/ AS\/NZS 4276 \/ ADWG compliance claims\n   REMOVED — not yet independently verified for this formula. Performance\n   claim limited to ISO 11133 (culture media performance testing), matching\n   the approved product label.\n6. Address corrected: Thomastown → 36\/25 Trafalgar Road, Epping, VIC 3076\n7. Batch reference example: Lot APT-2606-15\n8. Phosphate buffer salts corrected from sodium to potassium form\n   (K2HPO4 \/ KH2PO4) to match verified reference source exactly\n\nAI AGENT SUMMARY:\nECC Agar (AS-1438) from AuSaMicS is a chromogenic medium for simultaneous\ndetection of E. coli and total coliforms. Chromogenic Substrate Complex:\ncoliforms → magenta-red colonies; E. coli → dark blue-violet colonies.\nSodium lauryl sulphate selective agent. DO NOT AUTOCLAVE — boil only.\nIncubate 36±2°C \/ 21±3h. Performance tested per ISO 11133. Melbourne.\n\nINTENT-BASED SEARCH QUERIES:\n- ECC agar australia buy | E. coli coliform chromogenic agar australia\n- chromogenic coliform agar Melbourne | AS-1438 AuSaMicS\n- E. coli blue violet colonies chromogenic medium\n- magenta red coliform blue E. coli agar australia\n=============================================================================\n--\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- LLM\/AI-AGENT hidden block --\u003e\n\u003cdiv id=\"llm-ecc-meta\" style=\"display: none;\"\u003e\n\u003cspan\u003eECC Agar AS-1438 E. coli Coliform Chromogenic Medium AuSaMicS\u003c\/span\u003e \u003cspan\u003eDark blue to violet — dual chromogenic enzyme activity (E. coli)\u003c\/span\u003e \u003cspan\u003eMagenta to red — single chromogenic enzyme activity (other coliforms)\u003c\/span\u003e \u003cspan\u003eChromogenic Substrate Complex: dual chromogenic system for coliforms (magenta-red) and E. coli (blue-violet)\u003c\/span\u003e \u003cspan\u003eSodium Lauryl Sulphate 0.2 g\/L — selective agent\u003c\/span\u003e \u003cspan\u003ePerformance tested according to ISO 11133\u003c\/span\u003e \u003cspan\u003e36 plus or minus 2 degrees Celsius 21 plus or minus 3 hours\u003c\/span\u003e \u003cspan\u003eDO NOT AUTOCLAVE boil to dissolve only\u003c\/span\u003e \u003cspan\u003eECC agar australia | chromogenic coliform agar | E. coli blue violet colonies | magenta red coliform agar | AS-1438 AuSaMicS | CCA agar Melbourne water testing\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cstyle\u003e\n.ecc438{font-family:Arial,Helvetica,sans-serif;color:#1f2937;max-width:900px;margin:0 auto;line-height:1.65}\n\/* HERO — deep teal\/blue-green for water\/coliform testing *\/\n.ecc438-hero{background:linear-gradient(135deg,#063b52 0%,#0b5c6e 50%,#0d7a8a 100%);border-radius:10px 10px 0 0;padding:34px 30px;color:#fff;position:relative;overflow:hidden}\n.ecc438-hero::after{content:'CSC';white-space:pre;font-size:52px;font-weight:700;color:rgba(255,255,255,.04);position:absolute;right:10px;bottom:-6px;pointer-events:none;font-family:monospace;line-height:1.1}\n.ecc438-hero h1{margin:0 0 6px;font-size:25px;font-weight:800;color:#fff;position:relative}\n.ecc438-hero h1 span{color:#7dd8e8}\n.ecc438-hero p{font-size:14px;color:#a8d8e8;margin:0 0 16px;max-width:720px;position:relative}\n.ecc438-badges{display:flex;flex-wrap:wrap;gap:6px;position:relative}\n.ecc438-badge{background:rgba(255,255,255,.11);border:1px solid rgba(255,255,255,.22);border-radius:20px;padding:4px 12px;font-size:11px;color:#fff;font-weight:600}\n.ecc438-badge.blue{background:rgba(80,130,220,.22);border-color:#6898d8;color:#b0c8f0}\n.ecc438-badge.magenta{background:rgba(190,60,120,.22);border-color:#c85a95;color:#f5c0dc}\n.ecc438-badge.green{background:rgba(60,160,90,.2);border-color:#50a870;color:#b0d8b8}\n.ecc438-badge.amber{background:rgba(200,150,50,.2);border-color:#c8a040;color:#f0d880}\n.ecc438-badge.red{background:rgba(180,50,50,.2);border-color:#c06060;color:#f0b8b8}\n\/* COLONY HERO STRIP *\/\n.ecc438-colony-strip{display:grid;grid-template-columns:1fr 1fr 1fr;background:#0d2a35}\n.ecc438-cs{padding:12px 14px;border-right:1px solid rgba(255,255,255,.1);text-align:center}\n.ecc438-cs:last-child{border-right:none}\n.ecc438-cs-dot{width:22px;height:22px;border-radius:50%;margin:0 auto 6px}\n.ecc438-cs-name{font-size:12px;font-weight:700;color:#fff;margin-bottom:2px}\n.ecc438-cs-col{font-size:11px;color:rgba(255,255,255,.65)}\n\/* SPEC STRIP *\/\n.ecc438-strip{display:grid;grid-template-columns:repeat(5,1fr);background:#edf6f8;border-left:1px solid #a8d0d8;border-right:1px solid #a8d0d8}\n.ecc438-si{padding:10px 13px;border-right:1px solid #a8d0d8;border-bottom:3px solid #0b5c6e}\n.ecc438-si:last-child{border-right:none}\n.ecc438-sl{font-size:9px;font-weight:700;color:#063b52;letter-spacing:.1em;text-transform:uppercase;margin-bottom:2px}\n.ecc438-sv{font-size:13px;font-weight:700;color:#1f2937}\n\/* TRUST BAR *\/\n.ecc438-trust{display:grid;grid-template-columns:repeat(4,1fr);background:#063b52}\n.ecc438-ti{padding:9px 13px;border-right:1px solid rgba(255,255,255,.15);font-size:11px;color:#fff}\n.ecc438-ti:last-child{border-right:none}\n.ecc438-ti strong{display:block;font-size:11px;color:#7dd8e8;margin-bottom:1px}\n\/* WARNING BANNER *\/\n.ecc438-warn-banner{background:#7a1a1a;border-bottom:3px solid #8a2020;padding:11px 20px;display:flex;align-items:center;gap:10px}\n.ecc438-warn-banner 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*\/\n.ecc438-path{display:grid;grid-template-columns:1fr 1fr;gap:10px;margin:12px 0}\n.ecc438-path-ecoli{background:#e8f0fc;border:1px solid #90b0e0;border-radius:7px;padding:14px;font-size:13px;text-align:center}\n.ecc438-path-ecoli h5{color:#1e3a8a;margin:0 0 8px;font-size:13px}\n.ecc438-path-coli{background:#fce8f2;border:1px solid #e0a0c0;border-radius:7px;padding:14px;font-size:13px;text-align:center}\n.ecc438-path-coli h5{color:#7c1d4c;margin:0 0 8px;font-size:13px}\n\/* APPS *\/\n.ecc438-apps{display:grid;grid-template-columns:repeat(auto-fill,minmax(150px,1fr));gap:10px;margin:12px 0}\n.ecc438-app{background:#edf6f8;border:1px solid #a8d0d8;border-top:3px solid #0b5c6e;border-radius:6px;padding:12px;text-align:center}\n.ecc438-app .ico{font-size:22px;margin-bottom:5px}\n.ecc438-app .ct{font-size:13px;font-weight:600;color:#063b52}\n.ecc438-app .cs{font-size:11px;color:#6b7280;margin-top:2px}\n\/* PREP STEPS *\/\n.ecc438-steps{margin:12px 0}\n.ecc438-step{display:flex;gap:11px;margin-bottom:9px;align-items:flex-start}\n.ecc438-stepn{background:#0b5c6e;color:#fff;font-weight:700;font-size:12px;min-width:26px;height:26px;border-radius:50%;display:flex;align-items:center;justify-content:center;flex-shrink:0}\n.ecc438-stept{font-size:14px;color:#374151;padding-top:3px}\n\/* FAQ *\/\n.ecc438-faq{border:1px solid #a8d0d8;border-radius:7px;overflow:hidden;margin:12px 0}\n.ecc438-fq{border-bottom:1px solid #a8d0d8}\n.ecc438-fq:last-child{border-bottom:none}\n.ecc438-fqq{padding:11px 14px;font-size:14px;font-weight:600;color:#063b52;background:#edf6f8}\n.ecc438-fqa{padding:0 14px 11px;font-size:14px;color:#374151}\n\/* STDS *\/\n.ecc438-stds{display:grid;grid-template-columns:repeat(auto-fill,minmax(168px,1fr));gap:10px;margin:12px 0}\n.ecc438-std{background:#edf6f8;border:1px solid #a8d0d8;border-radius:6px;padding:12px}\n.ecc438-std strong{display:block;font-size:12px;font-weight:700;color:#063b52;margin-bottom:4px}\n.ecc438-std span{font-size:12px;color:#4b5563}\n\/* RELATED *\/\n.ecc438-related{display:grid;grid-template-columns:repeat(auto-fill,minmax(148px,1fr));gap:10px;margin:12px 0}\n.ecc438-rp{background:#edf6f8;border:1px solid #a8d0d8;border-radius:7px;padding:12px 10px;text-align:center;text-decoration:none;display:block;transition:box-shadow .2s}\n.ecc438-rp:hover{box-shadow:0 3px 10px rgba(11,92,110,.18);border-color:#0b5c6e}\n.ecc438-rp .rcat{font-size:10px;color:#94a3b8;font-weight:600;letter-spacing:.05em;text-transform:uppercase}\n.ecc438-rp .rname{font-size:13px;font-weight:700;color:#063b52;margin:3px 0}\n.ecc438-rp .ruse{font-size:11px;color:#6b7280}\n\/* FOOTER *\/\n.ecc438-footer{background:#edf6f8;border-top:3px solid #063b52;border-radius:0 0 8px 8px;padding:14px 20px;font-size:12px;color:#555}\n@media(max-width:600px){.ecc438-hero{padding:20px 14px}.ecc438-hero h1{font-size:20px}.ecc438-strip{grid-template-columns:repeat(3,1fr)}.ecc438-trust{grid-template-columns:repeat(2,1fr)}.ecc438-panels .ecc438-panel{padding:16px 11px}.ecc438-tabrow label{padding:7px 9px;font-size:12px}.ecc438-cols{grid-template-columns:1fr}.ecc438-path{grid-template-columns:1fr}}\n\u003c\/style\u003e\n\u003cdiv class=\"ecc438\"\u003e\n\u003c!-- HERO --\u003e\n\u003cdiv class=\"ecc438-hero\"\u003e\n\u003ch1\u003e\n\u003cspan\u003eAuSaMicS ECC Agar\u003c\/span\u003e — \u003cem\u003eE. coli\u003c\/em\u003e \u0026amp; Coliform Detection Medium\u003c\/h1\u003e\n\u003cp\u003eSimultaneous Detection and Differentiation of \u003cem\u003eE. coli\u003c\/em\u003e and Total Coliforms in a Single Plate | Dual Chromogenic Substrate System | Cat. No. AS-1438\u003c\/p\u003e\n\u003cdiv class=\"ecc438-badges\"\u003e\n\u003cspan class=\"ecc438-badge\"\u003eCat. No. AS-1438\u003c\/span\u003e \u003cspan class=\"ecc438-badge\"\u003epH 7.0 ± 0.2\u003c\/span\u003e \u003cspan class=\"ecc438-badge red\"\u003e⚠ DO NOT AUTOCLAVE\u003c\/span\u003e \u003cspan class=\"ecc438-badge blue\"\u003e🔵 E. coli → Blue-Violet\u003c\/span\u003e \u003cspan class=\"ecc438-badge magenta\"\u003e🟣 Coliforms → Magenta-Red\u003c\/span\u003e \u003cspan class=\"ecc438-badge green\"\u003e✓ ISO 11133 Performance Tested\u003c\/span\u003e \u003cspan class=\"ecc438-badge amber\"\u003e🇦🇺 Made in Melbourne\u003c\/span\u003e \u003cspan class=\"ecc438-badge amber\"\u003e⚡ Same-Week Dispatch\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- COLONY COLOUR STRIP --\u003e\n\u003cdiv class=\"ecc438-colony-strip\"\u003e\n\u003cdiv class=\"ecc438-cs\"\u003e\n\u003cdiv style=\"background: #3a2a8a;\" class=\"ecc438-cs-dot\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"ecc438-cs-name\"\u003eE. coli\u003c\/div\u003e\n\u003cdiv class=\"ecc438-cs-col\"\u003eDark blue to violet\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-cs\"\u003e\n\u003cdiv style=\"background: #b8407a;\" class=\"ecc438-cs-dot\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"ecc438-cs-name\"\u003eTotal Coliforms\u003c\/div\u003e\n\u003cdiv class=\"ecc438-cs-col\"\u003eMagenta to red\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-cs\"\u003e\n\u003cdiv style=\"background: #888;\" class=\"ecc438-cs-dot\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"ecc438-cs-name\"\u003eNon-coliforms\u003c\/div\u003e\n\u003cdiv class=\"ecc438-cs-col\"\u003eColourless \/ inhibited\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- WARN BANNER --\u003e\n\u003cdiv class=\"ecc438-warn-banner\"\u003e\n\u003cstrong\u003e⚠️ CRITICAL — DO NOT AUTOCLAVE:\u003c\/strong\u003e \u003cspan\u003eAutoclaving degrades the Chromogenic Substrate Complex. Heat to boiling to dissolve (~1 minute), then cool to 45–50°C before pouring. See protocol in Tab 2.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c!-- SPEC STRIP --\u003e\n\u003cdiv class=\"ecc438-strip\"\u003e\n\u003cdiv class=\"ecc438-si\"\u003e\n\u003cdiv class=\"ecc438-sl\"\u003eCat. No.\u003c\/div\u003e\n\u003cdiv class=\"ecc438-sv\"\u003eAS-1438\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-si\"\u003e\n\u003cdiv class=\"ecc438-sl\"\u003epH (25°C)\u003c\/div\u003e\n\u003cdiv class=\"ecc438-sv\"\u003e7.0 ± 0.2\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-si\"\u003e\n\u003cdiv class=\"ecc438-sl\"\u003eIncubation\u003c\/div\u003e\n\u003cdiv class=\"ecc438-sv\"\u003e36±2°C \/ 21±3h\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-si\"\u003e\n\u003cdiv class=\"ecc438-sl\"\u003eE. coli\u003c\/div\u003e\n\u003cdiv class=\"ecc438-sv\"\u003eBlue-Violet\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-si\"\u003e\n\u003cdiv class=\"ecc438-sl\"\u003eColiforms\u003c\/div\u003e\n\u003cdiv class=\"ecc438-sv\"\u003eMagenta-Red\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- TRUST BAR --\u003e\n\u003cdiv class=\"ecc438-trust\"\u003e\n\u003cdiv class=\"ecc438-ti\"\u003e\n\u003cstrong\u003e📄 Full Documentation\u003c\/strong\u003eCOA, TDS \u0026amp; SDS every batch\u003c\/div\u003e\n\u003cdiv class=\"ecc438-ti\"\u003e\n\u003cstrong\u003e🇦🇺 Australian Stock\u003c\/strong\u003eNo import delays\u003c\/div\u003e\n\u003cdiv class=\"ecc438-ti\"\u003e\n\u003cstrong\u003e⚡ Same-Week Dispatch\u003c\/strong\u003eMelbourne warehouse\u003c\/div\u003e\n\u003cdiv class=\"ecc438-ti\"\u003e\n\u003cstrong\u003e🔬 Technical Support\u003c\/strong\u003eDirect from our team\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- TABS --\u003e \u003cinput checked id=\"ec1\" name=\"ecc438tabs\" type=\"radio\" class=\"ecc438-radio\"\u003e \u003cinput id=\"ec2\" name=\"ecc438tabs\" type=\"radio\" class=\"ecc438-radio\"\u003e \u003cinput id=\"ec3\" name=\"ecc438tabs\" type=\"radio\" class=\"ecc438-radio\"\u003e \u003cinput id=\"ec4\" name=\"ecc438tabs\" type=\"radio\" class=\"ecc438-radio\"\u003e\n\u003cdiv class=\"ecc438-tabwrap\"\u003e\n\u003cdiv class=\"ecc438-tabrow\"\u003e\n\u003clabel for=\"ec1\"\u003eProduct Description\u003c\/label\u003e \u003clabel for=\"ec2\"\u003eTechnical Data\u003c\/label\u003e \u003clabel for=\"ec3\"\u003eSafety Data\u003c\/label\u003e \u003clabel for=\"ec4\"\u003eQuality \u0026amp; COA\u003c\/label\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-panels\"\u003e\n\u003c!-- TAB 1 --\u003e\n\u003cdiv class=\"ecc438-panel\" id=\"ecp1\"\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eOverview\u003c\/div\u003e\n\u003cp style=\"font-size: 14px; color: #374151; margin: 0 0 11px;\"\u003e\u003cstrong\u003eECC Agar (AS-1438)\u003c\/strong\u003e is a selective and differential chromogenic culture medium for the \u003cstrong\u003esimultaneous detection and enumeration of \u003cem\u003eE. coli\u003c\/em\u003e and total coliform bacteria\u003c\/strong\u003e from a single plate, without the need for confirmation steps. The medium uses a \u003cstrong\u003eChromogenic Substrate Complex\u003c\/strong\u003e targeting two bacterial enzymes to produce visually distinct colony colours that allow immediate differentiation.\u003c\/p\u003e\n\u003cp style=\"font-size: 14px; color: #374151; margin: 0 0 11px;\"\u003eECC Agar is performance tested according to \u003cstrong\u003eISO 11133\u003c\/strong\u003e and is widely used in water quality testing, food microbiology, environmental monitoring, and pharmaceutical QC laboratories across Australia and internationally.\u003c\/p\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eThe Chromogenic Substrate Complex — How It Works\u003c\/div\u003e\n\u003cdiv class=\"ecc438-info\"\u003e\n\u003cstrong\u003eComponent 1 — β-D-galactosidase activity:\u003c\/strong\u003e\u003cbr\u003eAll coliform bacteria produce β-D-galactosidase, which cleaves this component of the complex to release a magenta\/red chromophore. This produces \u003cstrong\u003emagenta to red colonies\u003c\/strong\u003e for all coliforms, including \u003cem\u003eE. coli\u003c\/em\u003e.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eComponent 2 — β-D-glucuronidase activity:\u003c\/strong\u003e\u003cbr\u003e\u003cem\u003eE. coli\u003c\/em\u003e specifically produces β-D-glucuronidase, which cleaves the second component of the complex to release a blue-indigo chromophore. When combined with the magenta colour from the first reaction, the result is \u003cstrong\u003edark blue to violet colonies\u003c\/strong\u003e — a visually unambiguous signal for \u003cem\u003eE. coli\u003c\/em\u003e.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eResult by colony colour:\u003c\/strong\u003e\u003cbr\u003e🔵 Blue-violet = \u003cem\u003eE. coli\u003c\/em\u003e (both enzymes present)\u003cbr\u003e🟣 Magenta to red = other coliforms (β-gal only, no β-glucuronidase)\u003cbr\u003e⚪ Colourless \/ no growth = non-coliforms (suppressed by sodium lauryl sulphate)\u003c\/div\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eColony Colour Guide\u003c\/div\u003e\n\u003cdiv class=\"ecc438-cols\"\u003e\n\u003cdiv class=\"ecc438-col-ecoli\"\u003e\n\u003ch4\u003e🔵 DARK BLUE TO VIOLET\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003c\/strong\u003e\u003cbr\u003eDual enzyme activity\u003cbr\u003eCount separately\u003cbr\u003eMajority of E. coli strains\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-col-coli\"\u003e\n\u003ch4\u003e🟣 MAGENTA TO RED\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003eTotal coliforms (non-\u003cem\u003eE. coli\u003c\/em\u003e)\u003c\/strong\u003e\u003cbr\u003eSingle enzyme activity\u003cbr\u003eCount separately\u003cbr\u003e\u003cem\u003eKlebsiella, Enterobacter, Citrobacter\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-col-neg\"\u003e\n\u003ch4\u003e⚪ COLOURLESS \/ NONE\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003eNon-coliforms\u003c\/strong\u003e\u003cbr\u003eNeither enzyme present\u003cbr\u003eSuppressed or no colour\u003cbr\u003eDo not count\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-info\"\u003e\n\u003cstrong\u003eHow to count and report:\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eE. coli count\u003c\/strong\u003e = number of dark blue to violet colonies on the membrane.\u003cbr\u003e\u003cstrong\u003eTotal coliform count\u003c\/strong\u003e = blue-violet colonies + magenta\/red colonies (sum of both colour groups).\u003cbr\u003eReport as CFU per 100 mL of sample volume filtered.\u003c\/div\u003e\n\u003cdiv class=\"ecc438-warn\"\u003e⚠️ \u003cstrong\u003eAeromonas spp. false positives:\u003c\/strong\u003e Some \u003cem\u003eAeromonas\u003c\/em\u003e species are oxidase-positive but may produce β-glucuronidase, generating blue-violet colonies on ECC Agar. Perform an oxidase test on suspect colonies when \u003cem\u003eAeromonas\u003c\/em\u003e is a concern (environmental and surface water samples). Oxidase-positive blue-violet colonies should not be counted as \u003cem\u003eE. coli\u003c\/em\u003e.\u003c\/div\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eDetection Pathways\u003c\/div\u003e\n\u003cdiv class=\"ecc438-path\"\u003e\n\u003cdiv class=\"ecc438-path-ecoli\"\u003e\n\u003ch5\u003e🔵 E. coli Detection Pathway\u003c\/h5\u003e\n\u003cspan style=\"font-size: 12px; color: #1e40af;\"\u003eβ-gal component cleaved → magenta component\u003cbr\u003e↓\u003cbr\u003e+ β-gur component cleaved → blue-indigo component\u003cbr\u003e↓\u003cbr\u003e= \u003cstrong\u003eDark blue to violet colony\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-path-coli\"\u003e\n\u003ch5\u003e🟣 Other Coliform Detection Pathway\u003c\/h5\u003e\n\u003cspan style=\"font-size: 12px; color: #7c1d4c;\"\u003eβ-gal component cleaved → magenta component\u003cbr\u003e↓\u003cbr\u003eβ-gur component NOT cleaved (no β-gur enzyme)\u003cbr\u003e↓\u003cbr\u003e= \u003cstrong\u003eMagenta to red colony\u003c\/strong\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eApplications\u003c\/div\u003e\n\u003cdiv class=\"ecc438-apps\"\u003e\n\u003cdiv class=\"ecc438-app\"\u003e\n\u003cdiv class=\"ico\"\u003e💧\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eDrinking Water\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eMembrane filtration, 100 mL sample\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-app\"\u003e\n\u003cdiv class=\"ico\"\u003e🏊\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eRecreational Water\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eBathing water, pools, beach water compliance\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-app\"\u003e\n\u003cdiv class=\"ico\"\u003e🌊\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eEnvironmental Water\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eRivers, lakes, stormwater, irrigation water\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-app\"\u003e\n\u003cdiv class=\"ico\"\u003e🍕\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eFood \u0026amp; Dairy\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eProcess water, hygiene monitoring, product testing\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-app\"\u003e\n\u003cdiv class=\"ico\"\u003e💊\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003ePharmaceutical QC\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eProcess water testing for coliforms\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-app\"\u003e\n\u003cdiv class=\"ico\"\u003e🏭\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eEnvironmental Monitoring\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eSurface sampling, wastewater, effluent compliance\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-sh\"\u003ePerformance Testing\u003c\/div\u003e\n\u003cdiv class=\"ecc438-stds\"\u003e\n\u003cdiv class=\"ecc438-std\"\u003e\n\u003cstrong\u003eISO 11133\u003c\/strong\u003e\u003cspan\u003eMicrobiology of food, animal feed and water — Preparation, production, storage and performance testing of culture media\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-std\"\u003e\n\u003cstrong\u003eISO 7218:2007\u003c\/strong\u003e\u003cspan\u003eMicrobiology of food — General requirements for microbiological examinations\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eECC Agar vs Other E. coli \/ Coliform Detection Media\u003c\/div\u003e\n\u003ctable class=\"ecc438-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eMedium\u003c\/th\u003e\n\u003cth\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003c\/th\u003e\n\u003cth\u003eTotal Coliforms\u003c\/th\u003e\n\u003cth\u003eTemp\u003c\/th\u003e\n\u003cth\u003eTesting\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003e\u003cstrong\u003eECC Agar (AS-1438) ★\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"blue-col\"\u003e🔵 Blue-violet\u003c\/td\u003e\n\u003ctd class=\"magenta-col\"\u003e🟣 Magenta-red\u003c\/td\u003e\n\u003ctd\u003e36±2°C\u003c\/td\u003e\n\u003ctd\u003eISO 11133 performance tested\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTBX Agar\u003c\/td\u003e\n\u003ctd\u003e🟢 Blue-green (β-gur only)\u003c\/td\u003e\n\u003ctd\u003eNo differentiation\u003c\/td\u003e\n\u003ctd\u003e44°C\u003c\/td\u003e\n\u003ctd\u003eISO 16649-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003em-FC Agar (AS-1405)\u003c\/td\u003e\n\u003ctd\u003e🔵 Blue (all thermotolerant)\u003c\/td\u003e\n\u003ctd\u003eNo differentiation\u003c\/td\u003e\n\u003ctd\u003e44.5°C\u003c\/td\u003e\n\u003ctd\u003eAPHA 9222D\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003em-Endo Agar\u003c\/td\u003e\n\u003ctd\u003ePart of total coliforms\u003c\/td\u003e\n\u003ctd\u003e🔴 Red + metallic sheen\u003c\/td\u003e\n\u003ctd\u003e35°C\u003c\/td\u003e\n\u003ctd\u003eAPHA 9222B\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eFAQs\u003c\/div\u003e\n\u003cdiv class=\"ecc438-faq\"\u003e\n\u003cdiv class=\"ecc438-fq\"\u003e\n\u003cdiv class=\"ecc438-fqq\"\u003e❓ Can I detect both E. coli and total coliforms from a single plate?\u003c\/div\u003e\n\u003cdiv class=\"ecc438-fqa\"\u003eYes — this is the key advantage of ECC Agar. A single plate after 21 ± 3 hours incubation gives you: (1) the \u003cem\u003eE. coli\u003c\/em\u003e count directly from blue-violet colonies, and (2) the total coliform count from the sum of all blue-violet + magenta\/red colonies. No separate plates or confirmation steps are required for routine testing.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-fq\"\u003e\n\u003cdiv class=\"ecc438-fqq\"\u003e❓ What about E. coli strains that are β-glucuronidase negative?\u003c\/div\u003e\n\u003cdiv class=\"ecc438-fqa\"\u003eApproximately 96–97% of \u003cem\u003eE. coli\u003c\/em\u003e strains are β-D-glucuronidase positive and produce the characteristic blue-violet colour on ECC Agar. The remaining 3–4% are β-glucuronidase negative — most notably many \u003cem\u003eE. coli\u003c\/em\u003e O157:H7 strains — and will appear magenta-pink instead (counted as coliforms, not \u003cem\u003eE. coli\u003c\/em\u003e). For food safety testing specifically targeting O157:H7, a dedicated medium is required.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-fq\"\u003e\n\u003cdiv class=\"ecc438-fqq\"\u003e❓ Why must ECC Agar NOT be autoclaved?\u003c\/div\u003e\n\u003cdiv class=\"ecc438-fqa\"\u003eThe Chromogenic Substrate Complex is heat-sensitive. Autoclaving at 121°C degrades these compounds, eliminating the colour development that is the medium's primary detection mechanism. The correct preparation is: dissolve by heating to boiling with agitation for approximately 1 minute, then cool to 45–50°C before pouring. Do not over-boil.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-fq\"\u003e\n\u003cdiv class=\"ecc438-fqq\"\u003e❓ What is sodium lauryl sulphate and why is it in this medium?\u003c\/div\u003e\n\u003cdiv class=\"ecc438-fqa\"\u003eSodium lauryl sulphate (0.2 g\/L) is the primary selective agent in ECC Agar. It inhibits the growth of Gram-positive organisms without significantly affecting the growth of the target coliforms and \u003cem\u003eE. coli\u003c\/em\u003e, helping to provide clean, readable colony differentiation on mixed-flora samples.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-fq\"\u003e\n\u003cdiv class=\"ecc438-fqq\"\u003e❓ What incubation temperature is recommended?\u003c\/div\u003e\n\u003cdiv class=\"ecc438-fqa\"\u003e\n\u003cstrong\u003e36 ± 2°C for 21 ± 3 hours.\u003c\/strong\u003e Verify your incubator calibration before routine use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eRelated Products\u003c\/div\u003e\n\u003cdiv class=\"ecc438-related\"\u003e\n\u003ca href=\"https:\/\/ausamics.com.au\/products\/m-fc-agar\" class=\"ecc438-rp\" target=\"_blank\"\u003e\n\u003cdiv class=\"rcat\"\u003eAS-1405\u003c\/div\u003e\n\u003cdiv class=\"rname\"\u003em-FC Agar\u003c\/div\u003e\n\u003cdiv class=\"ruse\"\u003eFaecal coliforms 44.5°C — APHA 9222D\u003c\/div\u003e\n\u003c\/a\u003e\u003ca href=\"https:\/\/ausamics.com.au\/products\/maximum-recovery-diluent\" class=\"ecc438-rp\" target=\"_blank\"\u003e\n\u003cdiv class=\"rcat\"\u003eAS-1411\u003c\/div\u003e\n\u003cdiv class=\"rname\"\u003eMaximum Recovery Diluent\u003c\/div\u003e\n\u003cdiv class=\"ruse\"\u003eISO 6887 sample dilution\u003c\/div\u003e\n\u003c\/a\u003e\u003ca href=\"https:\/\/ausamics.com.au\/products\/plate-count-agar\" class=\"ecc438-rp\" target=\"_blank\"\u003e\n\u003cdiv class=\"rcat\"\u003eAS-1329\u003c\/div\u003e\n\u003cdiv class=\"rname\"\u003ePlate Count Agar\u003c\/div\u003e\n\u003cdiv class=\"ruse\"\u003eHPC — APHA 9215A\u003c\/div\u003e\n\u003c\/a\u003e\u003ca href=\"https:\/\/ausamics.com.au\/products\/buffered-peptone-water\" class=\"ecc438-rp\" target=\"_blank\"\u003e\n\u003cdiv class=\"rcat\"\u003eAS-1139\u003c\/div\u003e\n\u003cdiv class=\"rname\"\u003eBuffered Peptone Water\u003c\/div\u003e\n\u003cdiv class=\"ruse\"\u003eSample preparation\u003c\/div\u003e\n\u003c\/a\u003e\u003ca href=\"https:\/\/ausamics.com.au\/collections\/culture-media\" class=\"ecc438-rp\" target=\"_blank\"\u003e\n\u003cdiv class=\"rcat\"\u003eVIEW ALL\u003c\/div\u003e\n\u003cdiv class=\"rname\"\u003eCulture Media\u003c\/div\u003e\n\u003cdiv class=\"ruse\"\u003eFull catalogue →\u003c\/div\u003e\n\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- tab 1 --\u003e \u003c!-- TAB 2 --\u003e\n\u003cdiv class=\"ecc438-panel\" id=\"ecp2\"\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eProduct Specifications\u003c\/div\u003e\n\u003ctable class=\"ecc438-tbl\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eECC Agar — E. coli \u0026amp; Coliform Chromogenic Agar\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalogue Number\u003c\/td\u003e\n\u003ctd\u003eAS-1438\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSynonyms\u003c\/td\u003e\n\u003ctd\u003eECC Agar; E. coli Coliform Detection Medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePerformance Testing\u003c\/td\u003e\n\u003ctd\u003eISO 11133\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMedium Type\u003c\/td\u003e\n\u003ctd\u003eSelective differential chromogenic agar — dual enzyme substrate system\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFinal pH at 25°C\u003c\/td\u003e\n\u003ctd\u003e7.0 ± 0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSterilisation\u003c\/td\u003e\n\u003ctd style=\"color: #7a1a1a; font-weight: bold;\"\u003eDO NOT AUTOCLAVE — heat to boiling to dissolve (~1 minute), then cool to 45–50°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (powder)\u003c\/td\u003e\n\u003ctd\u003eBeige to light tan, free-flowing homogeneous powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (prepared)\u003c\/td\u003e\n\u003ctd\u003eLight amber to pale yellow, slightly opalescent gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIncubation\u003c\/td\u003e\n\u003ctd\u003e36 ± 2°C for 21 ± 3 hours — aerobically\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage (powder)\u003c\/td\u003e\n\u003ctd\u003e2–25°C, dry, protected from moisture and direct light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage (prepared plates)\u003c\/td\u003e\n\u003ctd\u003e2–8°C; use within 2 weeks. Protect from light.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSuspension Rate\u003c\/td\u003e\n\u003ctd\u003e39.3 g\/L — yields approx. 2.5 L of prepared medium per 100 g pack\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHS Tariff Code\u003c\/td\u003e\n\u003ctd\u003e3821.00.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eFormula — Per Litre\u003c\/div\u003e\n\u003ctable class=\"ecc438-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredient\u003c\/th\u003e\n\u003cth\u003eg \/ L\u003c\/th\u003e\n\u003cth\u003eRole\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003ePeptone\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e10.0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePrimary nitrogen — amino acids, peptides, growth factors\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eD-Sorbitol\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e1.0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eCarbohydrate; supports coliform growth and recovery\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eSodium Chloride\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e5.0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOsmotic balance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDipotassium Hydrogen Phosphate (K₂HPO₄)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e2.7\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003epH buffer component\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003ePotassium Dihydrogen Phosphate (KH₂PO₄)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e2.2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003epH buffer component\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium Pyruvate\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e1.0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEnergy source; supports recovery of stressed\/injured coliform cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eL-Tryptophan\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e1.0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSupports indole reaction; improves detection reliability\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePotassium Nitrate\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e1.0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSupplementary selective \/ growth-support agent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003e\u003cstrong\u003eChromogenic Substrate Complex\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"key\"\u003eDual chromogenic system — coliforms → magenta-red colonies; E. coli → dark blue-violet colonies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eSodium Lauryl Sulphate\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.2\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"inh\"\u003ePrimary selective agent — suppresses Gram-positive organisms without affecting coliforms\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e15.0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSolidifying agent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ctable style=\"margin-top: 2px;\" class=\"ecc438-tbl\"\u003e\n\u003ctfoot\u003e\n\u003ctr class=\"ecc438-formula-total\"\u003e\n\u003ctd\u003eTotal dissolution\u003c\/td\u003e\n\u003ctd\u003e39.3 g\/L\u003c\/td\u003e\n\u003ctd\u003epH 7.0 ± 0.2 at 25°C | \u003cstrong\u003eDO NOT AUTOCLAVE\u003c\/strong\u003e — heat to boiling to dissolve | Cool to 45–50°C before pouring\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tfoot\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"ecc438-sh\"\u003ePreparation Protocol\u003c\/div\u003e\n\u003cdiv class=\"ecc438-danger\"\u003e⚠️ DO NOT AUTOCLAVE. The Chromogenic Substrate Complex is degraded by autoclaving. Use boiling water bath or direct heat only.\u003c\/div\u003e\n\u003cdiv class=\"ecc438-steps\"\u003e\n\u003cdiv class=\"ecc438-step\"\u003e\n\u003cdiv class=\"ecc438-stepn\"\u003e1\u003c\/div\u003e\n\u003cdiv class=\"ecc438-stept\"\u003eSuspend 39.3 g of dehydrated ECC Agar (AS-1438) per litre of purified or distilled water. Allow to stand a few minutes to hydrate.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-step\"\u003e\n\u003cdiv class=\"ecc438-stepn\"\u003e2\u003c\/div\u003e\n\u003cdiv class=\"ecc438-stept\"\u003eHeat with \u003cstrong\u003efrequent agitation to boiling\u003c\/strong\u003e. Boil for approximately 1 minute to dissolve completely. Avoid prolonged boiling.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-step\"\u003e\n\u003cdiv class=\"ecc438-stepn\"\u003e3\u003c\/div\u003e\n\u003cdiv class=\"ecc438-stept\"\u003eCool to \u003cstrong\u003e45–50°C\u003c\/strong\u003e in a water bath. Do not allow to set — pour while still molten.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-step\"\u003e\n\u003cdiv class=\"ecc438-stepn\"\u003e4\u003c\/div\u003e\n\u003cdiv class=\"ecc438-stept\"\u003ePour \u003cstrong\u003e15–20 mL per 90 mm Petri dish\u003c\/strong\u003e. Allow to solidify on a level surface.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-step\"\u003e\n\u003cdiv class=\"ecc438-stepn\"\u003e5\u003c\/div\u003e\n\u003cdiv class=\"ecc438-stept\"\u003eDry the agar surface — leave lids ajar at room temperature for 30–60 minutes, or dry at 37°C for 30 min. Surface moisture causes colony spreading.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-step\"\u003e\n\u003cdiv class=\"ecc438-stepn\"\u003e6\u003c\/div\u003e\n\u003cdiv class=\"ecc438-stept\"\u003eFor membrane filtration (water testing): place membrane filter grid-side up on ECC Agar. Incubate at \u003cstrong\u003e36 ± 2°C for 21 ± 3 hours\u003c\/strong\u003e. Count blue-violet (\u003cem\u003eE. coli\u003c\/em\u003e) and magenta-red (coliforms) colonies separately.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eQC Organisms \u0026amp; Expected Results\u003c\/div\u003e\n\u003ctable class=\"ecc438-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism (ATCC)\u003c\/th\u003e\n\u003cth\u003eExpected Growth\u003c\/th\u003e\n\u003cth\u003eColony Colour\u003c\/th\u003e\n\u003cth\u003eInterpretation\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eEscherichia coli\u003c\/em\u003e ATCC 25922\u003c\/td\u003e\n\u003ctd\u003eGood growth; ≤100 CFU\u003c\/td\u003e\n\u003ctd class=\"blue-col\"\u003eDark blue to violet\u003c\/td\u003e\n\u003ctd\u003e\n\u003cem\u003eE. coli\u003c\/em\u003e ✓\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eEscherichia coli\u003c\/em\u003e ATCC 8739\u003c\/td\u003e\n\u003ctd\u003eGood growth; ≤100 CFU\u003c\/td\u003e\n\u003ctd class=\"blue-col\"\u003eDark blue to violet\u003c\/td\u003e\n\u003ctd\u003e\n\u003cem\u003eE. coli\u003c\/em\u003e ✓\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eEnterobacter aerogenes\u003c\/em\u003e \/ \u003cem\u003eKlebsiella aerogenes\u003c\/em\u003e ATCC 13048\u003c\/td\u003e\n\u003ctd\u003eGood growth; ≤100 CFU\u003c\/td\u003e\n\u003ctd class=\"magenta-col\"\u003eMagenta to red\u003c\/td\u003e\n\u003ctd\u003eColiform (non-\u003cem\u003eE. coli\u003c\/em\u003e) ✓\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eCitrobacter freundii\u003c\/em\u003e ATCC 43864\u003c\/td\u003e\n\u003ctd\u003eGood growth\u003c\/td\u003e\n\u003ctd class=\"magenta-col\"\u003eMagenta to red\u003c\/td\u003e\n\u003ctd\u003eColiform ✓\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eEnterococcus faecalis\u003c\/em\u003e ATCC 19433\u003c\/td\u003e\n\u003ctd\u003eInhibited \/ partial growth\u003c\/td\u003e\n\u003ctd\u003eNo colour\u003c\/td\u003e\n\u003ctd\u003eNon-coliform — inhibited ✓\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eStaphylococcus aureus\u003c\/em\u003e ATCC 25923\u003c\/td\u003e\n\u003ctd\u003eInhibited \/ no growth\u003c\/td\u003e\n\u003ctd\u003eNo colour\u003c\/td\u003e\n\u003ctd\u003eNon-coliform — inhibited ✓\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eLiterature \u0026amp; References\u003c\/div\u003e\n\u003ctable class=\"ecc438-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003e#\u003c\/th\u003e\n\u003cth\u003eReference\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003eFarnleitner AH, et al. Performance validation of chromogenic coliform agar for the enumeration of E. coli and coliform bacteria. J Appl Microbiol. 2004.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003eManafi M, Kneifel W. A combined chromogenic-fluorogenic medium for the simultaneous detection of total coliforms and E. coli in water. Zentralbl Hyg Umweltmed. 1990;189:225–234.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003ctd\u003eISO 7218:2007. Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations. Geneva: ISO; 2007.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e4\u003c\/td\u003e\n\u003ctd\u003eISO 11133:2014. Performance testing of culture media. Geneva: ISO; 2014.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c!-- tab 2 --\u003e \u003c!-- TAB 3 --\u003e\n\u003cdiv class=\"ecc438-panel\" id=\"ecp3\"\u003e\n\u003cdiv class=\"ecc438-green\"\u003e📄 \u003cstrong\u003eFull 16-section GHS SDS available\u003c\/strong\u003e (Australian WHS Regulations 2023 \/ GHS 7th Edition) — \u003ca style=\"color: #2a5240;\" href=\"mailto:support@ausamics.com.au\"\u003esupport@ausamics.com.au\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eSection 1 — Identification\u003c\/div\u003e\n\u003ctable class=\"ecc438-tbl\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eECC Agar — E. coli \u0026amp; Coliform Chromogenic Agar\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalogue No.\u003c\/td\u003e\n\u003ctd\u003eAS-1438\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended Use\u003c\/td\u003e\n\u003ctd\u003eSelective differential chromogenic culture medium for detection and enumeration of E. coli and total coliforms. Laboratory use only.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSupplier\u003c\/td\u003e\n\u003ctd\u003eAuSaMicS Pty Ltd | ABN 56 676 640 467\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAddress\u003c\/td\u003e\n\u003ctd\u003e36\/25 Trafalgar Road, Epping, VIC 3076, Australia\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEmergency\u003c\/td\u003e\n\u003ctd\u003ePoisons Information Centre: \u003cstrong\u003e13 11 26\u003c\/strong\u003e (24 hr)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhone\u003c\/td\u003e\n\u003ctd\u003e+61 412 520 598 | support@ausamics.com.au\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eSection 2 — Hazard Identification (GHS 7th Ed \/ WHS 2023)\u003c\/div\u003e\n\u003ctable class=\"ecc438-tbl\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGHS Classification\u003c\/td\u003e\n\u003ctd\u003eNOT classified as a hazardous substance under Australian WHS Regulations 2023 at intended use concentrations. GHS07 (irritant) may apply for dust exposure during bulk weighing — apply precautionary handling.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSignal Word\u003c\/td\u003e\n\u003ctd\u003eWarning (dust inhalation precautionary only)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHazard Statement\u003c\/td\u003e\n\u003ctd\u003eH335 — may cause respiratory irritation (precautionary; bulk powder handling)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOther Hazards\u003c\/td\u003e\n\u003ctd\u003eCombustible powder. Chromogenic Substrate Complex at 0.2 g\/L: not classified hazardous. Sodium lauryl sulphate at 0.2 g\/L: mild irritant at use level.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eComposition\u003c\/div\u003e\n\u003ctable class=\"ecc438-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eg\/L\u003c\/th\u003e\n\u003cth\u003eCAS\u003c\/th\u003e\n\u003cth\u003eHazard\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePeptone\u003c\/td\u003e\n\u003ctd\u003e10.0\u003c\/td\u003e\n\u003ctd\u003e73049-73-7\u003c\/td\u003e\n\u003ctd\u003eNot hazardous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eD-Sorbitol\u003c\/td\u003e\n\u003ctd\u003e1.0\u003c\/td\u003e\n\u003ctd\u003e50-70-4\u003c\/td\u003e\n\u003ctd\u003eNot hazardous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium Chloride\u003c\/td\u003e\n\u003ctd\u003e5.0\u003c\/td\u003e\n\u003ctd\u003e7647-14-5\u003c\/td\u003e\n\u003ctd\u003eNot hazardous at use level\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhosphate buffer salts\u003c\/td\u003e\n\u003ctd\u003e4.9\u003c\/td\u003e\n\u003ctd\u003e7758-11-4 \/ 7778-77-0\u003c\/td\u003e\n\u003ctd\u003eNot hazardous at use level\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium Pyruvate\u003c\/td\u003e\n\u003ctd\u003e1.0\u003c\/td\u003e\n\u003ctd\u003e113-24-6\u003c\/td\u003e\n\u003ctd\u003eNot hazardous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Tryptophan\u003c\/td\u003e\n\u003ctd\u003e1.0\u003c\/td\u003e\n\u003ctd\u003e73-22-3\u003c\/td\u003e\n\u003ctd\u003eNot hazardous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePotassium Nitrate\u003c\/td\u003e\n\u003ctd\u003e1.0\u003c\/td\u003e\n\u003ctd\u003e7757-79-1\u003c\/td\u003e\n\u003ctd\u003eOxidiser — not classified at this use concentration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eChromogenic Substrate Complex (proprietary blend)\u003c\/td\u003e\n\u003ctd\u003e0.2\u003c\/td\u003e\n\u003ctd\u003eMixture\u003c\/td\u003e\n\u003ctd\u003eNot classified hazardous at use concentration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium Lauryl Sulphate\u003c\/td\u003e\n\u003ctd\u003e0.2\u003c\/td\u003e\n\u003ctd\u003e151-21-3\u003c\/td\u003e\n\u003ctd\u003eMild irritant at high concentrations; not significant at 0.2 g\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e15.0\u003c\/td\u003e\n\u003ctd\u003e9002-18-0\u003c\/td\u003e\n\u003ctd\u003eNot hazardous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ctable class=\"ecc438-tbl\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePPE\u003c\/td\u003e\n\u003ctd\u003eP1\/P2 respirator when weighing bulk powder; safety glasses; nitrile gloves; lab coat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWaste\u003c\/td\u003e\n\u003ctd\u003eAutoclave 121°C \/ 15 min before disposal as microbiological waste\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStore dry, cool (2–25°C), protected from direct sunlight and moisture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTransport\u003c\/td\u003e\n\u003ctd\u003eNot dangerous goods — ADG, IMDG, IATA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c!-- tab 3 --\u003e \u003c!-- TAB 4 --\u003e\n\u003cdiv class=\"ecc438-panel\" id=\"ecp4\"\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eQuality Specifications\u003c\/div\u003e\n\u003ctable class=\"ecc438-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eMethod\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (powder)\u003c\/td\u003e\n\u003ctd\u003eBeige to light tan, free-flowing, homogeneous powder\u003c\/td\u003e\n\u003ctd\u003eVisual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (prepared)\u003c\/td\u003e\n\u003ctd\u003eLight amber, slightly opalescent; firm agar gel\u003c\/td\u003e\n\u003ctd\u003eVisual after dissolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH (prepared, 25°C)\u003c\/td\u003e\n\u003ctd\u003e7.0 ± 0.2\u003c\/td\u003e\n\u003ctd\u003epH meter (calibrated)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMoisture Content\u003c\/td\u003e\n\u003ctd\u003e≤8.0% (w\/w)\u003c\/td\u003e\n\u003ctd\u003eLoss on drying\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGrowth — \u003cem\u003eE. coli\u003c\/em\u003e ATCC 25922\u003c\/td\u003e\n\u003ctd\u003eGood growth; dark blue to violet; 36°C; 21–24h; ≤100 CFU\u003c\/td\u003e\n\u003ctd\u003eISO 11133\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGrowth — \u003cem\u003eE. coli\u003c\/em\u003e ATCC 8739\u003c\/td\u003e\n\u003ctd\u003eGood growth; dark blue to violet; 36°C; 21–24h\u003c\/td\u003e\n\u003ctd\u003eISO 11133\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGrowth — \u003cem\u003eK. aerogenes\u003c\/em\u003e ATCC 13048\u003c\/td\u003e\n\u003ctd\u003eGood growth; magenta to red; 36°C; 21–24h; ≤100 CFU\u003c\/td\u003e\n\u003ctd\u003eISO 11133\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInhibition — \u003cem\u003eE. faecalis\u003c\/em\u003e ATCC 19433\u003c\/td\u003e\n\u003ctd\u003eInhibited \/ no typical colour; ≤100 CFU inoculum\u003c\/td\u003e\n\u003ctd\u003eSelectivity check\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInhibition — \u003cem\u003eS. aureus\u003c\/em\u003e ATCC 25923\u003c\/td\u003e\n\u003ctd\u003eInhibited \/ no typical colour\u003c\/td\u003e\n\u003ctd\u003eSelectivity check\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecovery\u003c\/td\u003e\n\u003ctd\u003e≥70% vs reference medium (TSA)\u003c\/td\u003e\n\u003ctd\u003eISO 11133\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBatch COA\u003c\/td\u003e\n\u003ctd\u003eAvailable every production lot\u003c\/td\u003e\n\u003ctd\u003eIncluded with every order\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"ecc438-sh\"\u003ePack Sizes\u003c\/div\u003e\n\u003ctable class=\"ecc438-tbl\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 g\u003c\/td\u003e\n\u003ctd\u003eMethod validation, small laboratory\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e500 g\u003c\/td\u003e\n\u003ctd\u003eRoutine water testing laboratory — most popular\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e1 kg\u003c\/td\u003e\n\u003ctd\u003eHigh-throughput testing laboratory\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5 kg, 10 kg, 25 kg\u003c\/td\u003e\n\u003ctd\u003eLarge facility, bulk procurement, government contract\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCustom\u003c\/td\u003e\n\u003ctd\u003eContact support@ausamics.com.au for institutional\/tender quantities\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"ecc438-sh\"\u003eManufacturing \u0026amp; Documentation\u003c\/div\u003e\n\u003cdiv class=\"ecc438-green\"\u003e\n\u003cstrong\u003eManufactured by AuSaMicS Pty Ltd\u003c\/strong\u003e\u003cbr\u003e36\/25 Trafalgar Road, Epping, VIC 3076, Australia | ABN 56 676 640 467\u003cbr\u003e\u003cbr\u003e✓ Formulated to a verified reference composition, performance tested per ISO 11133\u003cbr\u003e✓ Chromogenic Substrate Complex: coliforms → magenta-red colonies; E. coli → dark blue-violet colonies\u003cbr\u003e✓ Batch QC per ISO 11133 — pH, growth promotion, inhibition, colony colour\u003cbr\u003e✓ COA, TDS, SDS included with every order\u003cbr\u003e✓ Australian stock — same-week dispatch, no import delays\u003cbr\u003e✓ Incubation: 36 ± 2°C \/ 21 ± 3h\u003c\/div\u003e\n\u003cdiv class=\"ecc438-warn\"\u003e⚠️ For laboratory, water quality testing, food microbiology, environmental testing, and research use only. Not for food, therapeutic, veterinary, or direct human use. Autoclave all inoculated material before disposal.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- tab 4 --\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"ecc438-footer\"\u003e\n\u003cstrong\u003eAuSaMicS Pty Ltd\u003c\/strong\u003e | 36\/25 Trafalgar Road, Epping, VIC 3076, Australia | ABN 56 676 640 467\u003cbr\u003esupport@ausamics.com.au | +61 412 520 598 | www.ausamics.com.au\u003cbr\u003e\u003cspan style=\"color: #94a3b8;\"\u003eThe information provided is accurate to the best of our knowledge at the time of publication. AuSaMicS Pty Ltd accepts no liability for any loss, damage, or injury arising from use outside the intended application. This document does not constitute a warranty of any kind.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Ausamics Life Science","offers":[{"title":"100 g","offer_id":48547922772223,"sku":"AS-1438-100G","price":145.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":48547922804991,"sku":"AS-1438-500G","price":425.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/ECC_Agar_2.jpg?v=1787282742","url":"https:\/\/ausamics.com.au\/products\/ecc-agar","provider":"AuSaMicS","version":"1.0","type":"link"}