{"product_id":"emb-broth","title":"EMB Broth | Eosin Methylene Blue Medium for Coliform Detection | AS‑1229","description":"\u003c!-- LLM\/AI-Agent Optimization Meta Snippet --\u003e\n\u003c!--\n  product: EMB Broth (Eosin Methylene Blue Broth)\n  catalog_number: AS-1229\n  manufacturer: AuSaMicS Pty Ltd\n  cas: N\/A (culture media formulation)\n  category: Selective \u0026 Differential Culture Media | Gram-Negative Enteric Detection\n  target_organism: Gram-negative enteric bacteria, E. coli, coliforms\n  application: Water testing, food microbiology, clinical microbiology, coliform differentiation\n  standard_method: APHA Standard Methods, ISO 9308, USP \u003c61\u003e\n  equivalents: Oxoid CM0069, BD Difco 221060, Merck 1.10678, Thermo Fisher R455212\n  url: https:\/\/ausamics.com.au\/products\/emb-broth\n  supplier_country: Australia\n  stock_status: In Stock\n--\u003e\n\u003c!-- DuckDuckGo Instant Answer \/ Schema.org structured data --\u003e\n\u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\/\",\n  \"@type\": \"Product\",\n  \"name\": \"EMB Broth (Eosin Methylene Blue Broth)\",\n  \"alternateName\": [\"Eosin Methylene Blue Broth\", \"EMB Liquid Medium\", \"Levine EMB Broth\"],\n  \"description\": \"Selective and differential liquid medium for detection and differentiation of gram-negative enteric bacteria, particularly E. coli and coliforms. Eosin Y and methylene blue dyes inhibit gram-positive organisms and provide differential colour response.\",\n  \"sku\": \"AS-1229\",\n  \"brand\": { \"@type\": \"Brand\", \"name\": \"AuSaMicS\" },\n  \"offers\": {\n    \"@type\": \"Offer\",\n    \"availability\": \"https:\/\/schema.org\/InStock\",\n    \"areaServed\": \"AU\",\n    \"url\": \"https:\/\/ausamics.com.au\/products\/emb-broth\"\n  },\n  \"manufacturer\": {\n    \"@type\": \"Organization\",\n    \"name\": \"AuSaMicS Pty Ltd\",\n    \"address\": { \"@type\": \"PostalAddress\", \"streetAddress\": \"31 Longview CT\", \"addressLocality\": \"Thomastown\", \"addressRegion\": \"VIC\", \"postalCode\": \"3074\", \"addressCountry\": \"AU\" }\n  }\n}\n\u003c\/script\u003e\n\n\u003c!-- Intent-based long-tail search queries this page targets:\n  - EMB broth eosin methylene blue Australia buy\n  - eosin methylene blue broth gram negative detection\n  - EMB broth coliform differentiation water testing\n  - Levine EMB broth dehydrated powder Australia\n  - EMB liquid medium E. coli isolation\n  - selective differential broth gram negative enteric\n  - Oxoid CM0069 equivalent Australia\n  - EMB broth USP pharmaceutical sterility testing\n  - coliform selective broth food microbiology Australia\n--\u003e\n\n\u003cdiv style=\"font-family: 'Segoe UI', system-ui, -apple-system, sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\" class=\"aus-emb-product\"\u003e\n\n\u003c!-- Hero Section --\u003e\n\u003cdiv style=\"background: linear-gradient(135deg, #f5f0fb 0%, #ede0f7 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #cda8e8;\" class=\"aus-emb-hero-section\"\u003e\n  \u003cdiv class=\"aus-emb-hero-grid\" style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\"\u003e\n    \u003cdiv\u003e\n      \u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #6b21a8; font-weight: bold; margin-bottom: 12px; background: #ede0f7; padding: 6px 12px; border-radius: 20px;\"\u003eAuSaMicS Life Science • Selective \u0026amp; Differential Culture Media\u003c\/div\u003e\n      \u003ch1 style=\"color: #3b0764; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003eEMB Broth\u003c\/h1\u003e\n      \u003ch2 style=\"color: #6b21a8; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: 0.9;\"\u003eEosin Methylene Blue Broth\u003c\/h2\u003e\n      \u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #4a1772; max-width: 620px; font-weight: 500;\"\u003eClassic \u003cstrong\u003eselective and differential liquid medium\u003c\/strong\u003e for detection and differentiation of gram-negative enteric bacteria. Eosin Y and methylene blue dyes deliver powerful gram-positive suppression and distinctive colour responses — a cornerstone of water, food, and pharmaceutical microbiology.\u003c\/p\u003e\n      \u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n        \u003cspan style=\"background: #6b21a8; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eCat #: AS-1229\u003c\/span\u003e\n        \u003cspan style=\"background: #fff; border: 2px solid #6b21a8; color: #6b21a8; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAPHA \/ ISO Compliant\u003c\/span\u003e\n        \u003cspan style=\"background: #28a745; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e✓ Australian Stock\u003c\/span\u003e\n        \u003cspan style=\"background: #7c3aed; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🟣 Dual-Dye Selectivity\u003c\/span\u003e\n      \u003c\/div\u003e\n      \u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #4a1772;\"\u003e\n        \u003cspan\u003e🚀 \u003cstrong\u003eSame-Day Dispatch:\u003c\/strong\u003e Australian Stock\u003c\/span\u003e\n        \u003cspan\u003e🧫 \u003cstrong\u003eSelective \u0026amp; Differential:\u003c\/strong\u003e Gram-negative enteric ID\u003c\/span\u003e\n        \u003cspan\u003e🌡️ \u003cstrong\u003e35–37°C Incubation:\u003c\/strong\u003e 24–48 hours\u003c\/span\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003c!-- Guarantee Panel --\u003e\n    \u003cdiv class=\"aus-emb-guarantee\" style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.10); min-width: 280px; border: 1px solid #cda8e8;\"\u003e\n      \u003ch4 style=\"color: #3b0764; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003e🏆 Enteric Detection Specialist\u003c\/h4\u003e\n      \u003cdiv style=\"font-size: 14px; color: #4a5568; text-align: center; line-height: 1.5;\"\u003e\n        \u003cdiv style=\"margin-bottom: 12px;\"\u003e🟣 \u003cstrong\u003eDual-Dye System\u003c\/strong\u003e — Eosin Y + Methylene Blue\u003c\/div\u003e\n        \u003cdiv style=\"margin-bottom: 12px;\"\u003e🔬 \u003cstrong\u003eSelective \u0026amp; Differential\u003c\/strong\u003e — Single medium, dual function\u003c\/div\u003e\n        \u003cdiv style=\"margin-bottom: 12px;\"\u003e📋 \u003cstrong\u003eComplete Package\u003c\/strong\u003e — COA + SDS included\u003c\/div\u003e\n        \u003cdiv style=\"margin-bottom: 12px;\"\u003e⚡ \u003cstrong\u003eFast Supply\u003c\/strong\u003e — Ships within 24h\u003c\/div\u003e\n        \u003cdiv style=\"margin-bottom: 12px;\"\u003e📞 \u003cstrong\u003eExpert Support\u003c\/strong\u003e — Protocol optimization\u003c\/div\u003e\n      \u003c\/div\u003e\n      \u003cdiv style=\"margin-top: 20px; padding: 15px; background: #f5f0fb; border-radius: 12px; text-align: center; border: 1px solid #cda8e8;\"\u003e\n        \u003cdiv style=\"color: #6b21a8; font-weight: 600; font-size: 14px;\"\u003eLevine Formulation\u003c\/div\u003e\n        \u003cdiv style=\"color: #4a5568; font-size: 13px; margin-top: 5px;\"\u003eLactose + sucrose dual-sugar for broader coliform differentiation\u003c\/div\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- Main Content Grid --\u003e\n\u003cdiv class=\"aus-emb-main-grid\" style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\"\u003e\n  \u003cdiv\u003e\n\n    \u003c!-- Technical Overview \u0026 Biochemistry --\u003e\n    \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n      \u003ch3 style=\"color: #3b0764; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #6b21a8;\"\u003e🔬 Technical Overview \u0026amp; Biochemistry\u003c\/h3\u003e\n      \u003cdiv style=\"background: #f5f0fb; padding: 20px; border-radius: 16px; border-left: 5px solid #6b21a8;\"\u003e\n        \u003cp style=\"margin: 0 0 15px 0;\"\u003eEMB Broth is a classic \u003cstrong\u003eselective and differential liquid medium\u003c\/strong\u003e widely used for the detection, isolation, and preliminary differentiation of gram-negative enteric bacteria — particularly coliforms and \u003cem\u003eE. coli\u003c\/em\u003e — in water, food, environmental, and pharmaceutical samples.\u003c\/p\u003e\n        \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; font-size: 14px; color: #4a1772; border: 1px solid #cda8e8; margin-bottom: 15px;\"\u003e\n          \u003cstrong\u003eDual-Dye Selectivity \u0026amp; Differential Mechanism:\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e\n          \u003cstrong\u003eEosin Y\u003c\/strong\u003e — Anionic dye; inhibits gram-positive organisms by interfering with cell wall integrity; forms coloured complexes with acid end-products of fermentation\u003cbr\u003e\u003cbr\u003e\n          \u003cstrong\u003eMethylene Blue\u003c\/strong\u003e — Acts synergistically with Eosin Y for gram-positive inhibition; enhances differential colour contrast between fermenters and non-fermenters\u003cbr\u003e\u003cbr\u003e\n          \u003cstrong\u003eLactose \u0026amp; Sucrose (Levine formulation)\u003c\/strong\u003e — Fermentable carbohydrates; rapid acid producers (e.g., \u003cem\u003eE. coli\u003c\/em\u003e) yield turbidity with purplish-black precipitate; slow fermenters and non-fermenters remain clear or lightly turbid\n        \u003c\/div\u003e\n        \u003cdiv style=\"background: #ede0f7; padding: 12px; border-radius: 8px; border: 1px solid #cda8e8;\"\u003e\n          \u003cstrong style=\"color: #6b21a8;\"\u003eResult Interpretation:\u003c\/strong\u003e Turbid growth with dark purple\/black precipitate = strong acid producer (typical \u003cem\u003eE. coli\u003c\/em\u003e). Turbid without precipitate = coliform \/ gram-negative. Clear = inhibited or no growth (gram-positive suppressed).\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003c!-- Mode of Action --\u003e\n    \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n      \u003ch3 style=\"color: #3b0764; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #6b21a8;\"\u003e⚙️ Mode of Action\u003c\/h3\u003e\n      \u003cdiv style=\"background: #f5f0fb; padding: 20px; border-radius: 16px; border-left: 5px solid #7c3aed;\"\u003e\n        \u003cp style=\"margin: 0; font-size: 15px; color: #4a1772;\"\u003eEosin Y and methylene blue form a complex at low pH that precipitates within colonies or broth producing acid from lactose\/sucrose fermentation. Gram-positive organisms are inhibited because the dyes disrupt their cell membrane at the concentrations used, preventing growth. Gram-negative enteric bacteria tolerate the dye system and grow freely — with rapid acid producers such as \u003cem\u003eE. coli\u003c\/em\u003e generating sufficient pH drop to trigger a visible dark precipitate, while non-fermenting gram-negatives remain undifferentiated by turbidity alone.\u003c\/p\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003c!-- Selective \u0026 Differential Media Comparison --\u003e\n    \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n      \u003ch3 style=\"color: #3b0764; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #6b21a8;\"\u003e📊 Gram-Negative Enteric Media Comparison\u003c\/h3\u003e\n      \u003cdiv style=\"overflow-x: auto;\"\u003e\n        \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #cda8e8; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05); min-width: 800px;\"\u003e\n          \u003cthead style=\"background: #ede0f7;\"\u003e\n            \u003ctr\u003e\n              \u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #cda8e8; text-align: left; font-weight: bold; color: #3b0764;\"\u003eMedium\u003c\/th\u003e\n              \u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #cda8e8; text-align: left; font-weight: bold; color: #3b0764;\"\u003eType\u003c\/th\u003e\n              \u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #cda8e8; text-align: left; font-weight: bold; color: #3b0764;\"\u003eTarget\u003c\/th\u003e\n              \u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #cda8e8; text-align: left; font-weight: bold; color: #3b0764;\"\u003ePositive Signal\u003c\/th\u003e\n              \u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #cda8e8; text-align: left; font-weight: bold; color: #3b0764;\"\u003eKey Use\u003c\/th\u003e\n            \u003c\/tr\u003e\n          \u003c\/thead\u003e\n          \u003ctbody\u003e\n            \u003ctr style=\"background: #f5f0fb; border-bottom: 1px solid #cda8e8;\"\u003e\n              \u003ctd style=\"padding: 12px; font-weight: bold; color: #6b21a8;\"\u003eEMB Broth \u003cspan style=\"background: #6b21a8; color: #fff; font-size: 10px; padding: 2px 6px; border-radius: 10px; margin-left: 5px;\"\u003eTHIS PRODUCT\u003c\/span\u003e\n\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eSelective + Differential\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eGram-negative enterics \/ coliforms\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px; color: #6b21a8; font-weight: bold;\"\u003e🟣 Dark purple-black precipitate\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eWater, food, pharma, environmental\u003c\/td\u003e\n            \u003c\/tr\u003e\n            \u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n              \u003ctd style=\"padding: 12px;\"\u003e\u003ca style=\"color: #6b21a8; text-decoration: none; font-weight: 600;\" href=\"\/products\/macconkey-broth\"\u003eMacConkey Broth\u003c\/a\u003e\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eSelective + Differential\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eTotal coliforms\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px; color: #dc3545; font-weight: 600;\"\u003e🔴 Acid + gas (Durham tube)\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eWater, food, MPN method\u003c\/td\u003e\n            \u003c\/tr\u003e\n            \u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n              \u003ctd style=\"padding: 12px;\"\u003e\u003ca style=\"color: #6b21a8; text-decoration: none; font-weight: 600;\" href=\"\/products\/lauryl-tryptose-broth\"\u003eLauryl Tryptose Broth\u003c\/a\u003e\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eSelective + Presumptive\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eTotal coliforms\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px; color: #856404; font-weight: 600;\"\u003e🟡 Gas production (Durham tube)\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eAPHA water MPN presumptive\u003c\/td\u003e\n            \u003c\/tr\u003e\n            \u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n              \u003ctd style=\"padding: 12px;\"\u003e\u003ca style=\"color: #6b21a8; text-decoration: none; font-weight: 600;\" href=\"\/products\/ec-broth\"\u003eEC Broth\u003c\/a\u003e\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eSelective + Confirmatory\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003e\n\u003cem\u003eE. coli\u003c\/em\u003e (fecal coliforms)\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px; color: #17a2b8; font-weight: 600;\"\u003e💙 Gas + MUG fluorescence\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eMPN confirmatory at 44.5°C\u003c\/td\u003e\n            \u003c\/tr\u003e\n            \u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n              \u003ctd style=\"padding: 12px;\"\u003e\u003ca style=\"color: #6b21a8; text-decoration: none; font-weight: 600;\" href=\"\/products\/m-fc-broth\"\u003em-FC Broth\u003c\/a\u003e\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eSelective\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eFecal coliforms\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px; color: #0d6efd; font-weight: 600;\"\u003e🔵 Blue colonies (MF method)\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eMembrane filtration 44.5°C\u003c\/td\u003e\n            \u003c\/tr\u003e\n            \u003ctr\u003e\n              \u003ctd style=\"padding: 12px;\"\u003e\u003ca style=\"color: #6b21a8; text-decoration: none; font-weight: 600;\" href=\"\/products\/brilliant-green-bile-broth\"\u003eBrilliant Green Bile Broth\u003c\/a\u003e\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eSelective + Confirmatory\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eTotal coliforms (confirmed)\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px; color: #198754; font-weight: 600;\"\u003e🟢 Gas production (Durham tube)\u003c\/td\u003e\n              \u003ctd style=\"padding: 12px;\"\u003eMPN confirmatory step\u003c\/td\u003e\n            \u003c\/tr\u003e\n          \u003c\/tbody\u003e\n        \u003c\/table\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003c!-- Applications --\u003e\n    \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n      \u003ch3 style=\"color: #3b0764; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #6b21a8;\"\u003e🧬 Applications\u003c\/h3\u003e\n      \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(280px, 1fr)); gap: 20px; margin-bottom: 25px;\"\u003e\n        \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #cda8e8; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n          \u003ch4 style=\"color: #6b21a8; margin: 0 0 10px 0; font-size: 16px;\"\u003e💧 Water Quality Testing\u003c\/h4\u003e\n          \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eDetection and presumptive differentiation of coliform bacteria in potable, recreational, and environmental water samples — supports MPN multi-tube methodology.\u003c\/p\u003e\n        \u003c\/div\u003e\n        \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #cda8e8; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n          \u003ch4 style=\"color: #6b21a8; margin: 0 0 10px 0; font-size: 16px;\"\u003e🍽️ Food Microbiology\u003c\/h4\u003e\n          \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eEnrichment and selective detection of gram-negative enteric contaminants in food products, food processing surfaces, and ingredients — dairy, meat, produce, and ready-to-eat foods.\u003c\/p\u003e\n        \u003c\/div\u003e\n        \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #cda8e8; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n          \u003ch4 style=\"color: #6b21a8; margin: 0 0 10px 0; font-size: 16px;\"\u003e💊 Pharmaceutical QC\u003c\/h4\u003e\n          \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eNon-sterile product bioburden testing and \u003cem\u003eE. coli\u003c\/em\u003e absence tests per USP \u0026lt;61\u0026gt;, BP, and TGA requirements — liquid medium format suits enrichment steps.\u003c\/p\u003e\n        \u003c\/div\u003e\n        \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #cda8e8; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n          \u003ch4 style=\"color: #6b21a8; margin: 0 0 10px 0; font-size: 16px;\"\u003e🏭 Industrial Microbiology\u003c\/h4\u003e\n          \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eProcess water, cooling water, and environmental monitoring in manufacturing environments where gram-negative enteric control is critical.\u003c\/p\u003e\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n      \u003cdiv style=\"background: #ede0f7; padding: 20px; border-radius: 16px; border-left: 5px solid #6b21a8;\"\u003e\n        \u003ch4 style=\"color: #3b0764; margin: 0 0 15px 0; font-size: 16px;\"\u003eAdditional Laboratory Applications:\u003c\/h4\u003e\n        \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(250px, 1fr)); gap: 15px;\"\u003e\n          \u003cul style=\"margin: 0; padding-left: 20px; color: #4a5568; font-size: 14px;\"\u003e\n            \u003cli\u003e\n\u003cstrong\u003eClinical Microbiology:\u003c\/strong\u003e Selective enrichment prior to plating on differential agar\u003c\/li\u003e\n            \u003cli\u003e\n\u003cstrong\u003eResearch:\u003c\/strong\u003e Coliform ecology, antibiotic resistance, and E. coli population studies\u003c\/li\u003e\n            \u003cli\u003e\n\u003cstrong\u003eTeaching Labs:\u003c\/strong\u003e Classical gram-negative differentiation demonstrations\u003c\/li\u003e\n          \u003c\/ul\u003e\n          \u003cul style=\"margin: 0; padding-left: 20px; color: #4a5568; font-size: 14px;\"\u003e\n            \u003cli\u003e\n\u003cstrong\u003eVeterinary Diagnostics:\u003c\/strong\u003e Enteric pathogen detection in animal samples\u003c\/li\u003e\n            \u003cli\u003e\n\u003cstrong\u003eEnvironmental Monitoring:\u003c\/strong\u003e Soil leachate and irrigation water assessment\u003c\/li\u003e\n            \u003cli\u003e\n\u003cstrong\u003eNATA Accredited Labs:\u003c\/strong\u003e Routine coliform testing workflows\u003c\/li\u003e\n          \u003c\/ul\u003e\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003c!-- Procedure --\u003e\n    \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n      \u003ch3 style=\"color: #3b0764; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #6b21a8;\"\u003e📋 Recommended Procedure\u003c\/h3\u003e\n      \u003cdiv style=\"background: #f5f0fb; padding: 20px; border-radius: 16px; border-left: 5px solid #6b21a8;\"\u003e\n        \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(200px, 1fr)); gap: 15px; margin-bottom: 15px;\"\u003e\n          \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 10px; border: 1px solid #cda8e8; text-align: center;\"\u003e\n            \u003cdiv style=\"font-size: 24px; margin-bottom: 8px;\"\u003e1️⃣\u003c\/div\u003e\n            \u003cstrong style=\"color: #3b0764; font-size: 14px;\"\u003ePrepare Medium\u003c\/strong\u003e\n            \u003cp style=\"font-size: 13px; color: #4a5568; margin: 6px 0 0 0;\"\u003eDissolve 20.0 g\/L in purified water. Mix well. Dispense into tubes with Durham tubes if gas detection required.\u003c\/p\u003e\n          \u003c\/div\u003e\n          \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 10px; border: 1px solid #cda8e8; text-align: center;\"\u003e\n            \u003cdiv style=\"font-size: 24px; margin-bottom: 8px;\"\u003e2️⃣\u003c\/div\u003e\n            \u003cstrong style=\"color: #3b0764; font-size: 14px;\"\u003eSterilise\u003c\/strong\u003e\n            \u003cp style=\"font-size: 13px; color: #4a5568; margin: 6px 0 0 0;\"\u003eAutoclave at 121°C for 15 min. Allow to cool to room temperature before inoculation.\u003c\/p\u003e\n          \u003c\/div\u003e\n          \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 10px; border: 1px solid #cda8e8; text-align: center;\"\u003e\n            \u003cdiv style=\"font-size: 24px; margin-bottom: 8px;\"\u003e3️⃣\u003c\/div\u003e\n            \u003cstrong style=\"color: #3b0764; font-size: 14px;\"\u003eInoculate\u003c\/strong\u003e\n            \u003cp style=\"font-size: 13px; color: #4a5568; margin: 6px 0 0 0;\"\u003eAdd sample (water, food homogenate, or swab rinse). Use 1:10 ratio for food samples.\u003c\/p\u003e\n          \u003c\/div\u003e\n          \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 10px; border: 1px solid #cda8e8; text-align: center;\"\u003e\n            \u003cdiv style=\"font-size: 24px; margin-bottom: 8px;\"\u003e4️⃣\u003c\/div\u003e\n            \u003cstrong style=\"color: #3b0764; font-size: 14px;\"\u003eIncubate\u003c\/strong\u003e\n            \u003cp style=\"font-size: 13px; color: #4a5568; margin: 6px 0 0 0;\"\u003e35–37°C for 24–48 h. Examine at 24 h and again at 48 h if negative.\u003c\/p\u003e\n          \u003c\/div\u003e\n          \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 10px; border: 1px solid #cda8e8; text-align: center;\"\u003e\n            \u003cdiv style=\"font-size: 24px; margin-bottom: 8px;\"\u003e5️⃣\u003c\/div\u003e\n            \u003cstrong style=\"color: #3b0764; font-size: 14px;\"\u003eInterpret \u0026amp; Subculture\u003c\/strong\u003e\n            \u003cp style=\"font-size: 13px; color: #4a5568; margin: 6px 0 0 0;\"\u003eTurbid with dark precipitate = positive. Subculture to EMB Agar or confirmatory media for full identification.\u003c\/p\u003e\n          \u003c\/div\u003e\n        \u003c\/div\u003e\n        \u003cdiv style=\"background: #e8f5ee; padding: 12px; border-radius: 8px; border: 1px solid #a8d5bb;\"\u003e\n          \u003cstrong style=\"color: #1a6b3a;\"\u003e💡 Tip:\u003c\/strong\u003e \u003cspan style=\"font-size: 14px; color: #1a6b3a;\"\u003eFor MPN water testing, use triple-strength EMB Broth in the first dilution tube (1 mL sample into 5 mL triple-strength broth) to avoid dilution of selectivity. Pair with EMB Agar for definitive colony morphology confirmation.\u003c\/span\u003e\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003c!-- Protocol Optimization --\u003e\n    \u003cdiv style=\"background: linear-gradient(135deg, #fff3e0 0%, #ffe0b3 100%); padding: 20px; border-radius: 16px; border-left: 5px solid #ff9800;\"\u003e\n      \u003ch4 style=\"color: #e65100; margin: 0 0 15px 0; font-size: 16px;\"\u003e💡 Protocol Optimization Guidelines\u003c\/h4\u003e\n      \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(220px, 1fr)); gap: 15px;\"\u003e\n        \u003cdiv\u003e\n          \u003cstrong style=\"color: #e65100;\"\u003ePreparation:\u003c\/strong\u003e\u003cbr\u003e\n          \u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eDissolve 20.0 g\/L in purified water. Autoclave 121°C \/ 15 min. Avoid overheating — excessive heat darkens the medium and reduces selectivity.\u003c\/span\u003e\n        \u003c\/div\u003e\n        \u003cdiv\u003e\n          \u003cstrong style=\"color: #e65100;\"\u003epH (25°C):\u003c\/strong\u003e\u003cbr\u003e\n          \u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003e7.1 ± 0.2 after sterilisation\u003c\/span\u003e\n        \u003c\/div\u003e\n        \u003cdiv\u003e\n          \u003cstrong style=\"color: #e65100;\"\u003eTriple-Strength Variant:\u003c\/strong\u003e\u003cbr\u003e\n          \u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eDissolve 60.0 g\/L for use in first-tube MPN series when inoculating large sample volumes (≥1 mL).\u003c\/span\u003e\n        \u003c\/div\u003e\n        \u003cdiv\u003e\n          \u003cstrong style=\"color: #e65100;\"\u003eStorage (prepared):\u003c\/strong\u003e\u003cbr\u003e\n          \u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003e2–8°C, use within 2 weeks. Protect from light — dyes are photosensitive. Dehydrated powder: below 25°C, dry and dark.\u003c\/span\u003e\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n\n  \u003c\/div\u003e\n\u003c!-- end left column --\u003e\n\n  \u003c!-- Right Column --\u003e\n  \u003cdiv\u003e\n\n    \u003c!-- Technical Specifications --\u003e\n    \u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #cda8e8; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n      \u003ch3 style=\"color: #3b0764; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003e📋 Technical Specifications\u003c\/h3\u003e\n      \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px;\"\u003e\n        \u003ctbody\u003e\n          \u003ctr style=\"border-bottom: 1px solid #cda8e8;\"\u003e\n            \u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #3b0764; width: 45%; background: #f5f0fb;\"\u003eCatalogue Number\u003c\/td\u003e\n            \u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eAS-1229\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #cda8e8;\"\u003e\n            \u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #3b0764; background: #f5f0fb;\"\u003eFull Name\u003c\/td\u003e\n            \u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eEosin Methylene Blue Broth (Levine)\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #cda8e8;\"\u003e\n            \u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #3b0764; background: #f5f0fb;\"\u003eFormat\u003c\/td\u003e\n            \u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eDehydrated powder\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #cda8e8;\"\u003e\n            \u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #3b0764; background: #f5f0fb;\"\u003eReconstitution\u003c\/td\u003e\n            \u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e20.0 g\/L in purified water\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #cda8e8;\"\u003e\n            \u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #3b0764; background: #f5f0fb;\"\u003epH (25°C)\u003c\/td\u003e\n            \u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e7.1 ± 0.2\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #cda8e8;\"\u003e\n            \u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #3b0764; background: #f5f0fb;\"\u003eIncubation Temp\u003c\/td\u003e\n            \u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #6b21a8;\"\u003e35–37°C\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #cda8e8;\"\u003e\n            \u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #3b0764; background: #f5f0fb;\"\u003eIncubation Time\u003c\/td\u003e\n            \u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e24–48 hours\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #cda8e8;\"\u003e\n            \u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #3b0764; background: #f5f0fb;\"\u003ePositive Result\u003c\/td\u003e\n            \u003ctd style=\"padding: 12px 8px; color: #6b21a8; font-weight: 600;\"\u003eTurbid + dark purple-black precipitate\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #cda8e8;\"\u003e\n            \u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #3b0764; background: #f5f0fb;\"\u003eNegative Result\u003c\/td\u003e\n            \u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eClear \/ no turbidity\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #cda8e8;\"\u003e\n            \u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #3b0764; background: #f5f0fb;\"\u003eSelective Agents\u003c\/td\u003e\n            \u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eEosin Y, Methylene Blue\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #cda8e8;\"\u003e\n            \u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #3b0764; background: #f5f0fb;\"\u003eSterilisation\u003c\/td\u003e\n            \u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eAutoclave 121°C \/ 15 min\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #cda8e8;\"\u003e\n            \u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #3b0764; background: #f5f0fb;\"\u003eFormulation Type\u003c\/td\u003e\n            \u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eLevine (lactose + sucrose)\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #3b0764; background: #f5f0fb;\"\u003eHS Code\u003c\/td\u003e\n            \u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e3821.00.00\u003c\/td\u003e\n          \u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n\n    \u003c!-- QC \/ Performance Data --\u003e\n    \u003cdiv style=\"background: #ede0f7; padding: 20px; border-radius: 16px; border: 1px solid #cda8e8; margin-bottom: 30px;\"\u003e\n      \u003ch4 style=\"color: #6b21a8; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e✅ Quality Control Performance\u003c\/h4\u003e\n      \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px;\"\u003e\n        \u003cthead\u003e\n          \u003ctr style=\"background: #d8b4f8;\"\u003e\n            \u003cth style=\"padding: 8px; text-align: left; color: #3b0764; border-bottom: 1px solid #cda8e8;\"\u003eTest Organism\u003c\/th\u003e\n            \u003cth style=\"padding: 8px; text-align: left; color: #3b0764; border-bottom: 1px solid #cda8e8;\"\u003eATCC\u003c\/th\u003e\n            \u003cth style=\"padding: 8px; text-align: left; color: #3b0764; border-bottom: 1px solid #cda8e8;\"\u003eExpected Result\u003c\/th\u003e\n            \u003cth style=\"padding: 8px; text-align: left; color: #3b0764; border-bottom: 1px solid #cda8e8;\"\u003eCFU Spec\u003c\/th\u003e\n          \u003c\/tr\u003e\n        \u003c\/thead\u003e\n        \u003ctbody\u003e\n          \u003ctr style=\"border-bottom: 1px solid #c4b5d4;\"\u003e\n            \u003ctd style=\"padding: 8px; color: #4a1772;\"\u003e\u003cem\u003eEscherichia coli\u003c\/em\u003e\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e25922\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #6b21a8; font-weight: 600;\"\u003eTurbid + dark precipitate\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e≤100 CFU\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #c4b5d4;\"\u003e\n            \u003ctd style=\"padding: 8px; color: #4a1772;\"\u003e\u003cem\u003eKlebsiella pneumoniae\u003c\/em\u003e\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e13883\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #6b21a8; font-weight: 600;\"\u003eTurbid growth\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e≤100 CFU\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #c4b5d4;\"\u003e\n            \u003ctd style=\"padding: 8px; color: #4a1772;\"\u003e\u003cem\u003eEnterococcus faecalis\u003c\/em\u003e\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e19433\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #dc3545; font-weight: 600;\"\u003eInhibited \/ no growth\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e≤100 CFU\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd style=\"padding: 8px; color: #4a1772;\"\u003e\u003cem\u003eStaphylococcus aureus\u003c\/em\u003e\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e25923\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #dc3545; font-weight: 600;\"\u003eInhibited \/ no growth\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e≤100 CFU\u003c\/td\u003e\n          \u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n\n    \u003c!-- Formulation --\u003e\n    \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #cda8e8; margin-bottom: 30px;\"\u003e\n      \u003ch4 style=\"color: #3b0764; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e🧪 Typical Formulation (per litre)\u003c\/h4\u003e\n      \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px;\"\u003e\n        \u003ctbody\u003e\n          \u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n            \u003ctd style=\"padding: 8px; color: #4a1772; font-weight: 600;\"\u003ePeptone\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #4a5568; text-align: right;\"\u003e10.0 g\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n            \u003ctd style=\"padding: 8px; color: #4a1772; font-weight: 600;\"\u003eLactose\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #4a5568; text-align: right;\"\u003e5.0 g\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n            \u003ctd style=\"padding: 8px; color: #4a1772; font-weight: 600;\"\u003eSucrose\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #4a5568; text-align: right;\"\u003e5.0 g\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n            \u003ctd style=\"padding: 8px; color: #4a1772; font-weight: 600;\"\u003eDipotassium Hydrogen Phosphate\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #4a5568; text-align: right;\"\u003e2.0 g\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n            \u003ctd style=\"padding: 8px; color: #4a1772; font-weight: 600;\"\u003eEosin Y\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #4a5568; text-align: right;\"\u003e0.4 g\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd style=\"padding: 8px; color: #4a1772; font-weight: 600;\"\u003eMethylene Blue\u003c\/td\u003e\n            \u003ctd style=\"padding: 8px; color: #4a5568; text-align: right;\"\u003e0.065 g\u003c\/td\u003e\n          \u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n      \u003cdiv style=\"font-size: 12px; color: #6c757d; margin-top: 10px; font-style: italic;\"\u003e* Levine formulation (dual-sugar). Formulation may vary slightly between lot numbers. Refer to the current COA for exact composition.\u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003c!-- Standards Compliance --\u003e\n    \u003cdiv style=\"background: #e3f2fd; padding: 20px; border-radius: 16px; border: 1px solid #90caf9; margin-bottom: 30px;\"\u003e\n      \u003ch4 style=\"color: #1565c0; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e📜 Standards \u0026amp; Regulatory Compliance\u003c\/h4\u003e\n      \u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n        \u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eAPHA Standard Methods\u003c\/strong\u003e — Coliform detection MPN methodology\u003c\/li\u003e\n        \u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eISO 9308-2\u003c\/strong\u003e — Water quality, MPN method for \u003cem\u003eE. coli\u003c\/em\u003e and coliforms\u003c\/li\u003e\n        \u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eUSP \u0026lt;61\u0026gt; \/ \u0026lt;62\u0026gt;\u003c\/strong\u003e — Pharmaceutical microbial enumeration and absence tests\u003c\/li\u003e\n        \u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eTGA \/ BP\u003c\/strong\u003e — British Pharmacopoeia and TGA non-sterile product testing\u003c\/li\u003e\n        \u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eISO 21528\u003c\/strong\u003e — Horizontal methods for detection of Enterobacteriaceae\u003c\/li\u003e\n        \u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eNATA Accreditation\u003c\/strong\u003e — Suitable for NATA-accredited laboratories\u003c\/li\u003e\n      \u003c\/ul\u003e\n    \u003c\/div\u003e\n\n    \u003c!-- Alternative Names --\u003e\n    \u003cdiv style=\"background: #f5f0fb; padding: 20px; border-radius: 16px; border: 1px solid #cda8e8; margin-bottom: 30px;\"\u003e\n      \u003ch4 style=\"color: #6b21a8; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e📝 Alternative Names\u003c\/h4\u003e\n      \u003cdiv style=\"font-size: 14px; color: #6b21a8; text-align: center; line-height: 1.9;\"\u003e\n        \u003cdiv\u003e\u003cstrong\u003eEMB Broth\u003c\/strong\u003e\u003c\/div\u003e\n        \u003cdiv\u003e\u003cstrong\u003eEosin Methylene Blue Broth\u003c\/strong\u003e\u003c\/div\u003e\n        \u003cdiv\u003eLevine EMB Broth\u003c\/div\u003e\n        \u003cdiv\u003eEosin-Methylene Blue Liquid Medium\u003c\/div\u003e\n        \u003cdiv\u003eEMB Liquid Medium (Levine formulation)\u003c\/div\u003e\n        \u003cdiv\u003eGram-Negative Selective Broth\u003c\/div\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003c!-- Documentation --\u003e\n    \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #cda8e8;\"\u003e\n      \u003ch4 style=\"color: #3b0764; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e📁 Documentation\u003c\/h4\u003e\n      \u003cdiv style=\"display: flex; flex-direction: column; gap: 10px;\"\u003e\n        \u003ca href=\"\/pages\/request-a-quote\" style=\"display: flex; align-items: center; gap: 10px; padding: 12px; background: #f5f0fb; border-radius: 10px; text-decoration: none; border: 1px solid #cda8e8; color: #6b21a8; font-weight: 600; font-size: 14px;\"\u003e📄 Download Product Datasheet (TDS)\u003c\/a\u003e\n        \u003ca href=\"\/pages\/request-a-quote\" style=\"display: flex; align-items: center; gap: 10px; padding: 12px; background: #f5f0fb; border-radius: 10px; text-decoration: none; border: 1px solid #cda8e8; color: #6b21a8; font-weight: 600; font-size: 14px;\"\u003e⚠️ Download Safety Data Sheet (SDS)\u003c\/a\u003e\n        \u003ca href=\"\/pages\/request-a-quote\" style=\"display: flex; align-items: center; gap: 10px; padding: 12px; background: #f5f0fb; border-radius: 10px; text-decoration: none; border: 1px solid #cda8e8; color: #6b21a8; font-weight: 600; font-size: 14px;\"\u003e✅ Request Certificate of Analysis (COA)\u003c\/a\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n\n  \u003c\/div\u003e\n\u003c!-- end right column --\u003e\n\u003c\/div\u003e\n\n\u003c!-- Cross-Reference \/ Equivalent Products --\u003e\n\u003cdiv style=\"background: #f5f0fb; padding: 30px; border-radius: 18px; border: 1px solid #cda8e8; margin-bottom: 30px;\"\u003e\n  \u003ch3 style=\"color: #3b0764; margin: 0 0 20px 0; text-align: center; font-size: 20px;\"\u003e🔄 Cross-Reference \/ Equivalent Products\u003c\/h3\u003e\n  \u003cdiv style=\"overflow-x: auto;\"\u003e\n    \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #cda8e8; border-radius: 12px; overflow: hidden;\"\u003e\n      \u003cthead style=\"background: #ede0f7;\"\u003e\n        \u003ctr\u003e\n          \u003cth style=\"padding: 12px; text-align: left; color: #3b0764; font-weight: bold;\"\u003eSupplier\u003c\/th\u003e\n          \u003cth style=\"padding: 12px; text-align: left; color: #3b0764; font-weight: bold;\"\u003eProduct Name\u003c\/th\u003e\n          \u003cth style=\"padding: 12px; text-align: left; color: #3b0764; font-weight: bold;\"\u003eCatalogue Number\u003c\/th\u003e\n          \u003cth style=\"padding: 12px; text-align: left; color: #3b0764; font-weight: bold;\"\u003eNotes\u003c\/th\u003e\n        \u003c\/tr\u003e\n      \u003c\/thead\u003e\n      \u003ctbody\u003e\n        \u003ctr style=\"background: #f5f0fb; border-bottom: 1px solid #cda8e8;\"\u003e\n          \u003ctd style=\"padding: 12px; font-weight: bold; color: #6b21a8;\"\u003eAuSaMicS \u003cspan style=\"background: #6b21a8; color: #fff; font-size: 10px; padding: 2px 6px; border-radius: 10px; margin-left: 4px;\"\u003eAU Stock\u003c\/span\u003e\n\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eEMB Broth\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eAS-1229\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px; font-size: 12px; color: #6b21a8;\"\u003eAustralian sourced, same-day dispatch\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n          \u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eOxoid (Thermo Fisher)\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px;\"\u003eEosin Methylene Blue Broth\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px;\"\u003eCM0069\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px; font-size: 12px; color: #6c757d;\"\u003eEquivalent formulation\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n          \u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eBD Difco\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px;\"\u003eEMB Broth (Levine)\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px;\"\u003e221060\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px; font-size: 12px; color: #6c757d;\"\u003eEquivalent formulation\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n          \u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eMerck \/ Millipore\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px;\"\u003eEosin Methylene Blue Broth\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px;\"\u003e1.10678\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px; font-size: 12px; color: #6c757d;\"\u003eEquivalent formulation\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eThermo Fisher Scientific\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px;\"\u003eEMB Broth\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px;\"\u003eR455212\u003c\/td\u003e\n          \u003ctd style=\"padding: 12px; font-size: 12px; color: #6c757d;\"\u003eEquivalent formulation\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n  \u003cp style=\"font-size: 12px; color: #6c757d; margin-top: 12px; text-align: center; font-style: italic;\"\u003eCross-references are provided for convenience. Catalogue numbers are trademarks of their respective owners. AuSaMicS products are manufactured and quality-tested independently.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c!-- Related Products --\u003e\n\u003cdiv style=\"background: #f5f0fb; padding: 30px; border-radius: 18px; border: 1px solid #cda8e8; margin-bottom: 30px;\"\u003e\n  \u003ch3 style=\"color: #3b0764; margin: 0 0 20px 0; text-align: center; font-size: 20px;\"\u003e🧬 Complete Gram-Negative Enteric Detection System\u003c\/h3\u003e\n\n  \u003c!-- Selective \u0026 Differential Media --\u003e\n  \u003cdiv style=\"margin-bottom: 25px;\"\u003e\n    \u003ch4 style=\"color: #3b0764; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003eSelective \u0026amp; Differential Media\u003c\/h4\u003e\n    \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(175px, 1fr)); gap: 15px;\"\u003e\n      \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #cda8e8;\"\u003e\n        \u003ch5 style=\"color: #6b21a8; margin: 0 0 8px 0; font-size: 14px;\"\u003eEMB Agar\u003c\/h5\u003e\n        \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eMetallic sheen \u003cem\u003eE. coli\u003c\/em\u003e colonies\u003c\/p\u003e\n        \u003ca style=\"display: inline-block; background: #6b21a8; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/eosin-methylene-blue-agar\"\u003eView Product\u003c\/a\u003e\n      \u003c\/div\u003e\n      \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #cda8e8;\"\u003e\n        \u003ch5 style=\"color: #dc3545; margin: 0 0 8px 0; font-size: 14px;\"\u003eMacConkey Broth\u003c\/h5\u003e\n        \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eColiform MPN method\u003c\/p\u003e\n        \u003ca style=\"display: inline-block; background: #dc3545; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/macconkey-broth\"\u003eView Product\u003c\/a\u003e\n      \u003c\/div\u003e\n      \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #cda8e8;\"\u003e\n        \u003ch5 style=\"color: #856404; margin: 0 0 8px 0; font-size: 14px;\"\u003eLauryl Tryptose Broth\u003c\/h5\u003e\n        \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eAPHA presumptive coliform\u003c\/p\u003e\n        \u003ca style=\"display: inline-block; background: #ffc107; color: #212529; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/pages\/request-a-quote\"\u003eView Product\u003c\/a\u003e\n      \u003c\/div\u003e\n      \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #cda8e8;\"\u003e\n        \u003ch5 style=\"color: #198754; margin: 0 0 8px 0; font-size: 14px;\"\u003eBrilliant Green Bile Broth\u003c\/h5\u003e\n        \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eMPN confirmatory step\u003c\/p\u003e\n        \u003ca style=\"display: inline-block; background: #198754; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/brilliant-green-bile-broth-2-bgbb-or-brila-broth\"\u003eView Product\u003c\/a\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Water Testing Range --\u003e\n  \u003cdiv style=\"margin-bottom: 25px;\"\u003e\n    \u003ch4 style=\"color: #3b0764; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003eWater Testing \u0026amp; Membrane Filtration Media\u003c\/h4\u003e\n    \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(175px, 1fr)); gap: 15px;\"\u003e\n      \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #cda8e8;\"\u003e\n        \u003ch5 style=\"color: #0d6efd; margin: 0 0 8px 0; font-size: 14px;\"\u003em-FC Broth\u003c\/h5\u003e\n        \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eFecal coliform MF method\u003c\/p\u003e\n        \u003ca style=\"display: inline-block; background: #0d6efd; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/m-fc-broth\"\u003eView Product\u003c\/a\u003e\n      \u003c\/div\u003e\n      \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #cda8e8;\"\u003e\n        \u003ch5 style=\"color: #c0392b; margin: 0 0 8px 0; font-size: 14px;\"\u003em-Enterococcus Agar\u003c\/h5\u003e\n        \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eEnterococci MF method\u003c\/p\u003e\n        \u003ca style=\"display: inline-block; background: #c0392b; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/m-enterococcus-agar-slanetz-bartley-agar\"\u003eView Product\u003c\/a\u003e\n      \u003c\/div\u003e\n      \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #cda8e8;\"\u003e\n        \u003ch5 style=\"color: #17a2b8; margin: 0 0 8px 0; font-size: 14px;\"\u003eEC Broth\u003c\/h5\u003e\n        \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003e\u003cem\u003eE. coli\u003c\/em\u003e MPN confirmatory\u003c\/p\u003e\n        \u003ca style=\"display: inline-block; background: #17a2b8; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/ec-medium-broth\"\u003eView Product\u003c\/a\u003e\n      \u003c\/div\u003e\n      \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #cda8e8;\"\u003e\n        \u003ch5 style=\"color: #1a6b3a; margin: 0 0 8px 0; font-size: 14px;\"\u003eMUG Substrate\u003c\/h5\u003e\n        \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003e\u003cem\u003eE. coli\u003c\/em\u003e fluorogenic detection\u003c\/p\u003e\n        \u003ca style=\"display: inline-block; background: #1a6b3a; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/4-methylumbelliferyl-b-d-glucopyranosiduronic-acid-mug\"\u003eView Product\u003c\/a\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003cdiv style=\"text-align: center; margin-top: 20px;\"\u003e\n    \u003cdiv style=\"display: flex; justify-content: center; gap: 15px; flex-wrap: wrap;\"\u003e\n      \u003ca style=\"color: #6b21a8; font-weight: 600; text-decoration: none; border: 2px solid #6b21a8; padding: 10px 20px; border-radius: 8px;\" href=\"\/collections\/culture-media\"\u003e🔬 All Culture Media\u003c\/a\u003e\n      \u003ca style=\"color: #0d6efd; font-weight: 600; text-decoration: none; border: 2px solid #0d6efd; padding: 10px 20px; border-radius: 8px;\" href=\"\/pages\/request-a-quote\"\u003e💧 Water Testing Range\u003c\/a\u003e\n      \u003ca style=\"color: #6b21a8; font-weight: 600; text-decoration: none; border: 2px solid #6b21a8; padding: 10px 20px; border-radius: 8px;\" href=\"\/pages\/request-a-quote\"\u003e📋 Request a Quote\u003c\/a\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- Footer \/ Contact --\u003e\n\u003cdiv style=\"text-align: center; padding: 30px 0; border-top: 1px solid #cda8e8;\"\u003e\n  \u003cdiv style=\"margin-bottom: 20px;\"\u003e\n    \u003ch4 style=\"color: #3b0764; margin: 0 0 10px 0; font-size: 16px;\"\u003eNeed Coliform Detection Method Support?\u003c\/h4\u003e\n    \u003cp style=\"margin: 0; color: #6c757d; font-size: 14px;\"\u003eOur microbiologists can assist with MPN workflow design, USP \u0026lt;61\u0026gt; pharmaceutical testing setup, APHA water testing methods, and NATA compliance documentation.\u003c\/p\u003e\n  \u003c\/div\u003e\n  \u003cdiv style=\"display: flex; justify-content: center; gap: 20px; flex-wrap: wrap; margin-bottom: 25px;\"\u003e\n    \u003ca style=\"display: flex; align-items: center; gap: 8px; color: #6b21a8; text-decoration: none; font-weight: 600; padding: 12px 20px; border: 1px solid #6b21a8; border-radius: 10px;\" href=\"tel:+61412520598\"\u003e📞 +61 412 520 598\u003c\/a\u003e\n    \u003ca style=\"display: flex; align-items: center; gap: 8px; color: #6b21a8; text-decoration: none; font-weight: 600; padding: 12px 20px; border: 1px solid #6b21a8; border-radius: 10px;\" href=\"mailto:support@ausamics.com.au\"\u003e📧 Technical Support\u003c\/a\u003e\n  \u003c\/div\u003e\n  \u003cp style=\"margin: 0; font-size: 12px; color: #94a3b8; line-height: 1.6; font-style: italic; border: 1px solid #e2e8f0; padding: 12px 20px; border-radius: 8px; display: inline-block; max-width: 720px;\"\u003e\n    \u003cstrong\u003eFor laboratory, research, and industrial use only.\u003c\/strong\u003e Not for food, feed, household, cosmetic, therapeutic, or personal use. Results should be interpreted by qualified laboratory personnel in accordance with applicable standard methods.\u003cbr\u003e\u003cbr\u003e\n    AuSaMicS Pty Ltd | ABN: 56 676 640 467 | 31 Longview CT, Thomastown VIC 3074, Australia\u003cbr\u003e\n    www.ausamics.com.au | support@ausamics.com.au | +61 412 520 598\u003cbr\u003e\n    Same-day dispatch • Australian stock • Full documentation included\n  \u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\u003c!-- end aus-emb-product --\u003e\n\n\u003c!-- Mobile Responsiveness --\u003e\n\u003cstyle\u003e\n@media (max-width: 860px) {\n  .aus-emb-hero-grid, .aus-emb-main-grid {\n    grid-template-columns: 1fr !important;\n  }\n  .aus-emb-guarantee {\n    min-width: 100% !important;\n    margin-top: 20px;\n  }\n  .aus-emb-product {\n    padding: 15px !important;\n  }\n  .aus-emb-product h1 {\n    font-size: 24px !important;\n  }\n  .aus-emb-product h2 {\n    font-size: 18px !important;\n  }\n}\n@media (max-width: 640px) {\n  .aus-emb-hero-section {\n    padding: 25px !important;\n  }\n  .aus-emb-product table th,\n  .aus-emb-product table td {\n    padding: 8px 6px !important;\n  }\n}\n\u003c\/style\u003e","brand":"Ausamics Life Science","offers":[{"title":"100 g","offer_id":48374505832703,"sku":"AS-1229-100G","price":35.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":48374505865471,"sku":"AS-1229-500G","price":105.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/Eosin_Methylene_Blue_Medium_for_Coliform_Detection.jpg?v=1775541283","url":"https:\/\/ausamics.com.au\/products\/emb-broth","provider":"AuSaMicS","version":"1.0","type":"link"}