{"product_id":"enterococcosel-broth","title":"Enterococcosel Broth | Selective Medium for Enterococci Detection | AS‑1222","description":"\u003cdiv style=\"font-family: 'Segoe UI', system-ui, -apple-system, sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\" class=\"aus-enterococcoselbroth-product\"\u003e\n\n  \u003c!-- Hero Section --\u003e\n  \u003cdiv style=\"background: linear-gradient(135deg, #f8fbff 0%, #eef5fb 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #d8e1e8;\"\u003e\n    \u003cdiv style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\"\u003e\n\n      \u003cdiv\u003e\n        \u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #1d6fb8; font-weight: bold; margin-bottom: 12px; background: #e8f2ff; padding: 6px 12px; border-radius: 20px;\"\u003e\n          AuSaMicS Life Science • Culture Media\n        \u003c\/div\u003e\n\n        \u003ch1 style=\"color: #15324b; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003e\n          Enterococcosel Broth\n        \u003c\/h1\u003e\n\n        \u003ch2 style=\"color: #243b53; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: 0.9;\"\u003e\n          Selective Differential Broth for Detection of Enterococci via Esculin Hydrolysis\n        \u003c\/h2\u003e\n\n        \u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #334e68; max-width: 680px; font-weight: 500;\"\u003e\n          Enterococcosel Broth is a selective and differential medium designed for the \u003cstrong\u003edetection and presumptive identification of Enterococcus species\u003c\/strong\u003e. It combines \u003cstrong\u003ebile salt selectivity\u003c\/strong\u003e with \u003cstrong\u003eesculin hydrolysis detection\u003c\/strong\u003e, producing a characteristic \u003cstrong\u003eblackening reaction\u003c\/strong\u003e in the presence of esculin-positive organisms.\n        \u003c\/p\u003e\n\n        \u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n          \u003cspan style=\"background: #1d6fb8; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600;\"\u003eAS-1222\u003c\/span\u003e\n          \u003cspan style=\"background: #fff; border: 2px solid #1d6fb8; color: #1d6fb8; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600;\"\u003eEnterococcus Detection\u003c\/span\u003e\n          \u003cspan style=\"background: #28a745; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600;\"\u003e✓ Esculin Positive\u003c\/span\u003e\n          \u003cspan style=\"background: #6f42c1; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600;\"\u003eBile Selective\u003c\/span\u003e\n        \u003c\/div\u003e\n\n        \u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #4a5568;\"\u003e\n          \u003cspan\u003e🧪 Esculin + ferric iron indicator system\u003c\/span\u003e\n          \u003cspan\u003e🧫 Bile inhibition of non-enterococci\u003c\/span\u003e\n          \u003cspan\u003e🏥 Clinical \u0026amp; water microbiology applications\u003c\/span\u003e\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n\n      \u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 280px; border: 1px solid #d8e1e8;\"\u003e\n        \u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003e🏆 Enterococcus Screening Broth\u003c\/h4\u003e\n        \u003cdiv style=\"font-size: 14px; color: #4a5568; text-align: center; line-height: 1.5;\"\u003e\n          \u003cdiv style=\"margin-bottom: 12px;\"\u003e🦠 Selective for Group D streptococci\u003c\/div\u003e\n          \u003cdiv style=\"margin-bottom: 12px;\"\u003e⚗️ Strong blackening reaction\u003c\/div\u003e\n          \u003cdiv style=\"margin-bottom: 12px;\"\u003e📋 COA + SDS available\u003c\/div\u003e\n          \u003cdiv style=\"margin-bottom: 12px;\"\u003e💧 Suitable for environmental testing\u003c\/div\u003e\n          \u003cdiv style=\"margin-bottom: 12px;\"\u003e🔬 Reliable presumptive identification\u003c\/div\u003e\n        \u003c\/div\u003e\n        \u003cdiv style=\"margin-top: 20px; padding: 15px; background: #e3f2fd; border-radius: 12px; text-align: center; border: 1px solid #90caf9;\"\u003e\n          \u003cdiv style=\"color: #1565c0; font-weight: 600; font-size: 14px;\"\u003eKey Reaction\u003c\/div\u003e\n          \u003cdiv style=\"color: #4a5568; font-size: 13px; margin-top: 5px;\"\u003eBlackening of broth indicates esculin hydrolysis\u003c\/div\u003e\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Main Grid --\u003e\n  \u003cdiv style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\"\u003e\n\n    \u003cdiv\u003e\n\n      \u003c!-- Principle --\u003e\n      \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n        \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003e\n          🔬 Principle of the Medium\n        \u003c\/h3\u003e\n        \u003cdiv style=\"background: #f8fafc; padding: 20px; border-radius: 16px; border-left: 5px solid #1d6fb8;\"\u003e\n          \u003cp style=\"margin: 0 0 15px 0;\"\u003e\n            Enterococcosel Broth contains \u003cstrong\u003ebile salts\u003c\/strong\u003e to inhibit most Gram-positive organisms other than enterococci, and \u003cstrong\u003eesculin\u003c\/strong\u003e as a differential substrate. Enterococci hydrolyse esculin to esculetin, which reacts with \u003cstrong\u003eferric ions\u003c\/strong\u003e to produce a \u003cstrong\u003eblack complex\u003c\/strong\u003e.\n          \u003c\/p\u003e\n          \u003cp style=\"margin: 0 0 15px 0;\"\u003e\n            The combination of bile tolerance and esculin hydrolysis provides a reliable presumptive identification system for \u003cstrong\u003eEnterococcus spp. and Group D streptococci\u003c\/strong\u003e.\n          \u003c\/p\u003e\n          \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; font-family: monospace; font-size: 14px; color: #4a5568; border: 1px solid #d8e1e8; text-align: center;\"\u003e\n            Esculin\u003cbr\u003e\n            ↓ hydrolysis\u003cbr\u003e\n            Esculetin + dextrose\u003cbr\u003e\n            ↓ + ferric ions\u003cbr\u003e\n            Black precipitate (positive)\n          \u003c\/div\u003e\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n\n      \u003c!-- Applications --\u003e\n      \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n        \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003e\n          🧬 Applications\n        \u003c\/h3\u003e\n\n        \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(260px, 1fr)); gap: 20px;\"\u003e\n          \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d8e1e8;\"\u003e\n            \u003ch4 style=\"color: #007bff; margin: 0 0 10px 0;\"\u003e🏥 Clinical Microbiology\u003c\/h4\u003e\n            \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003e\n              Used for presumptive identification of enterococci from clinical specimens including urine, wound, and blood cultures.\n            \u003c\/p\u003e\n          \u003c\/div\u003e\n\n          \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d8e1e8;\"\u003e\n            \u003ch4 style=\"color: #007bff; margin: 0 0 10px 0;\"\u003e💧 Water Testing\u003c\/h4\u003e\n            \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003e\n              Suitable for detection and confirmation of enterococci in environmental and recreational water microbiology workflows.\n            \u003c\/p\u003e\n          \u003c\/div\u003e\n\n          \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d8e1e8;\"\u003e\n            \u003ch4 style=\"color: #007bff; margin: 0 0 10px 0;\"\u003e🍽️ Food Microbiology\u003c\/h4\u003e\n            \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003e\n              Used for monitoring enterococcal contamination in food products and production environments.\n            \u003c\/p\u003e\n          \u003c\/div\u003e\n\n          \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d8e1e8;\"\u003e\n            \u003ch4 style=\"color: #007bff; margin: 0 0 10px 0;\"\u003e🔬 QC \u0026amp; Research\u003c\/h4\u003e\n            \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003e\n              Useful in quality control workflows and research applications requiring reliable detection of bile-tolerant esculin-positive organisms.\n            \u003c\/p\u003e\n          \u003c\/div\u003e\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n\n      \u003c!-- Comparative Table --\u003e\n      \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n        \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003e\n          📊 Enterococcus Media Comparison\n        \u003c\/h3\u003e\n        \u003cdiv style=\"overflow-x: auto;\"\u003e\n          \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #d8e1e8;\"\u003e\n            \u003cthead style=\"background: #f6f8fb;\"\u003e\n              \u003ctr\u003e\n                \u003cth style=\"padding: 12px;\"\u003eMedium\u003c\/th\u003e\n                \u003cth style=\"padding: 12px;\"\u003eType\u003c\/th\u003e\n                \u003cth style=\"padding: 12px;\"\u003eRole\u003c\/th\u003e\n                \u003cth style=\"padding: 12px;\"\u003eKey Feature\u003c\/th\u003e\n              \u003c\/tr\u003e\n            \u003c\/thead\u003e\n            \u003ctbody\u003e\n              \u003ctr style=\"background: #e3f2fd;\"\u003e\n                \u003ctd style=\"padding: 12px; font-weight: bold;\"\u003eEnterococcosel Broth\u003c\/td\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eSelective \u0026amp; Differential\u003c\/td\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eDetection \u0026amp; confirmation\u003c\/td\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eBile + esculin blackening\u003c\/td\u003e\n              \u003c\/tr\u003e\n              \u003ctr\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eEnterococcosel Agar\u003c\/td\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eSelective \u0026amp; Differential\u003c\/td\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eIsolation\u003c\/td\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eSolid medium equivalent\u003c\/td\u003e\n              \u003c\/tr\u003e\n              \u003ctr\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eBile Esculin Agar\u003c\/td\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eSelective\u003c\/td\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eGroup D identification\u003c\/td\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eClassic bile-esculin test\u003c\/td\u003e\n              \u003c\/tr\u003e\n              \u003ctr\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eM-Enterococcus Agar\u003c\/td\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eSelective\u003c\/td\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eInitial recovery\u003c\/td\u003e\n                \u003ctd style=\"padding: 12px;\"\u003eUsed before confirmation\u003c\/td\u003e\n              \u003c\/tr\u003e\n            \u003c\/tbody\u003e\n          \u003c\/table\u003e\n        \u003c\/div\u003e\n      \u003c\/div\u003e\n\n      \u003c!-- Use Notes --\u003e\n      \u003cdiv style=\"background: #fff3e0; padding: 20px; border-radius: 16px; border-left: 5px solid #ff9800;\"\u003e\n        \u003ch4 style=\"color: #e65100;\"\u003e💡 Practical Notes\u003c\/h4\u003e\n        \u003cul style=\"margin: 10px 0 0 0; padding-left: 18px;\"\u003e\n          \u003cli\u003eBlackening indicates esculin-positive organisms\u003c\/li\u003e\n          \u003cli\u003eBile ensures selectivity against non-enterococci\u003c\/li\u003e\n          \u003cli\u003eUsed as presumptive (not final confirmatory) test\u003c\/li\u003e\n          \u003cli\u003eBest used alongside selective isolation media\u003c\/li\u003e\n        \u003c\/ul\u003e\n      \u003c\/div\u003e\n\n    \u003c\/div\u003e\n\n    \u003c!-- Right Column --\u003e\n    \u003cdiv\u003e\n\n      \u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #d8e1e8; margin-bottom: 30px;\"\u003e\n        \u003ch3 style=\"text-align:center;\"\u003e📋 Technical Specifications\u003c\/h3\u003e\n        \u003ctable style=\"width:100%; font-size:14px;\"\u003e\n          \u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eProduct\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eEnterococcosel Broth\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eCat No\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAS-1222\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eFormat\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eDehydrated powder\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eType\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSelective differential broth\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eKey Components\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBile salts, esculin, ferric citrate\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eReadout\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eBlackening reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e10–30°C dry\u003c\/td\u003e\n\u003c\/tr\u003e\n          \u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eHS Code\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e3821.00.00\u003c\/td\u003e\n\u003c\/tr\u003e\n        \u003c\/table\u003e\n      \u003c\/div\u003e\n\n      \u003cdiv style=\"background: #e3f2fd; padding: 20px; border-radius: 16px; margin-bottom: 30px;\"\u003e\n        \u003ch4 style=\"text-align:center;\"\u003e🧫 Interpretation\u003c\/h4\u003e\n        \u003cul style=\"list-style:none; padding:0;\"\u003e\n          \u003cli\u003e✓ Positive: Blackened broth\u003c\/li\u003e\n          \u003cli\u003e✓ Negative: No color change\u003c\/li\u003e\n          \u003cli\u003e✓ Indicates bile-tolerant esculin hydrolysis\u003c\/li\u003e\n        \u003c\/ul\u003e\n      \u003c\/div\u003e\n\n      \u003cdiv style=\"background: #e8f5e8; padding: 20px; border-radius: 16px;\"\u003e\n        \u003ch4 style=\"text-align:center;\"\u003e✅ Quality\u003c\/h4\u003e\n        \u003cul style=\"list-style:none; padding:0;\"\u003e\n          \u003cli\u003e✓ Consistent selectivity\u003c\/li\u003e\n          \u003cli\u003e✓ Clear differential reaction\u003c\/li\u003e\n          \u003cli\u003e✓ COA per lot\u003c\/li\u003e\n        \u003c\/ul\u003e\n      \u003c\/div\u003e\n\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Footer --\u003e\n  \u003cdiv style=\"background:#f8fafc; padding:20px; border-radius:12px; text-align:center;\"\u003e\n    \u003cp style=\"font-size:13px;\"\u003e\n      \u003cstrong\u003eDisclaimer:\u003c\/strong\u003e For laboratory and research use only.\n    \u003c\/p\u003e\n  \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"Ausamics Life Science","offers":[{"title":"500 g","offer_id":48391348158719,"sku":"AS-1222-500G","price":150.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/Enterococcosel_Broth.jpg?v=1776056312","url":"https:\/\/ausamics.com.au\/products\/enterococcosel-broth","provider":"AuSaMicS","version":"1.0","type":"link"}