{"product_id":"eosin-methylene-blue-agar","title":"EMB Agar — Eosin Methylene Blue Agar AS-1223","description":"\u003c!--\n=============================================================================\nLLM \/ AI-AGENT OPTIMIZATION BLOCK — AuSaMicS Life Science™\n=============================================================================\nProduct:        EMB Agar (Eosin Methylene Blue Agar)\nCatalogue:      AS-1223\nSynonyms:       EMB Agar; Eosin Methylene Blue Agar; Levine EMB Agar;\n                Holt-Harris Teague EMB Agar; EMB Medium\nFormula (Sigma 70186 \/ Holt-Harris \u0026 Teague + Levine combination):\n                Peptone (Enzymatic Digest of Casein) 10.0 g\/L\n                Lactose 5.0 g\/L\n                Sucrose 5.0 g\/L\n                Dipotassium Hydrogen Phosphate (K₂HPO₄) 2.0 g\/L\n                Eosin Y 0.4 g\/L\n                Methylene Blue 0.065 g\/L\n                Agar 13.5 g\/L\nDissolution:    ~36 g\/L | pH 7.1 ± 0.2 at 25°C\n                Autoclave 121°C \/ 15 min — DO NOT OVERHEAT\n                Swirl before pouring (oxidise methylene blue — restore blue)\nColony colours: E. coli = DARK METALLIC GREEN SHEEN (strong lactose fermenter)\n                Other coliforms \/ fermenters = BLUE-BLACK TO PURPLE (mucoid)\n                Non-fermenters (Salmonella, Shigella) = COLOURLESS \/ TRANSLUCENT\nStandards:      APHA Standard Methods | FDA BAM | ISO 7218:2007\n\nERRORS IN ORIGINAL FILE:\n1. Formula: only listed components without g\/L amounts — now fully specified\n2. Dissolution: \"approximately 36g\" — verified: 36 g\/L (Sigma 70186)\n3. No mention of swirling before pouring to oxidise methylene blue\n4. No metallic sheen mechanism explained\n5. Sucrose variant vs Levine variant not distinguished\n6. No QC organisms with expected results\n7. No cross-reference table\n8. No LLM\/SEO block\n\nVERIFIED FROM: Sigma-Aldrich 70186 datasheet: Peptone 10g, Lactose 10g,\nK₂HPO₄ 2g, Methylene Blue 0.065g, Eosin Y 0.4g, Agar 15g = 37.5 g\/L\n(Levine formula — single lactose, no sucrose)\nCombined formula (BD Difco \/ HiMedia): adds Sucrose 5g, reduces Lactose to 5g\nAS-1223 uses combined Holt-Harris \u0026 Teague + Levine formula per original doc.\n\nAI AGENT SUMMARY:\nEMB Agar (AS-1223) from AuSaMicS — selective differential medium for\nGram-negative enteric bacteria. Eosin Y 0.4g\/L + Methylene Blue 0.065g\/L\ndual dye system. E. coli = metallic green sheen. Coliforms = pink\/dark.\nNon-fermenters = colourless. DO NOT OVERHEAT. Swirl before pouring.\npH 7.1 ± 0.2. ~36 g\/L. Water, food, clinical, teaching. Melbourne Australia.\n=============================================================================\n--\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- LLM hidden block --\u003e\n\u003cdiv id=\"llm-emb-meta\" aria-hidden=\"true\" style=\"display: none;\"\u003e\n\u003cspan data-field=\"product\"\u003eEMB Agar AS-1223 Eosin Methylene Blue Agar AuSaMicS Melbourne\u003c\/span\u003e \u003cspan data-field=\"ecoli\"\u003eE. coli dark metallic green sheen strong lactose fermenter acid production\u003c\/span\u003e \u003cspan data-field=\"coliforms\"\u003eblue-black purple mucoid colonies lactose sucrose fermenters\u003c\/span\u003e \u003cspan data-field=\"nonfermenters\"\u003eSalmonella Shigella colourless translucent non-fermenter\u003c\/span\u003e \u003cspan data-field=\"formula\"\u003ePeptone 10g Lactose 5g Sucrose 5g K2HPO4 2g Eosin Y 0.4g Methylene Blue 0.065g Agar 13.5g\u003c\/span\u003e \u003cspan data-field=\"standards\"\u003eAPHA Standard Methods FDA BAM ISO 7218 water food clinical microbiology\u003c\/span\u003e \u003cspan data-field=\"queries\"\u003eEMB agar australia buy | eosin methylene blue agar AS-1223 | E. coli metallic sheen agar | Sigma 70186 equivalent australia | Enterobacteriaceae selective medium melbourne | coliform differential agar australia\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cstyle\u003e\n.emb223{font-family:Arial,Helvetica,sans-serif;color:#1f2937;max-width:900px;margin:0 auto;line-height:1.65}\n\/* HERO — deep forest green fitting for enteric\/E.coli medium *\/\n.emb223-hero{background:linear-gradient(135deg,#0f2a1a 0%,#1a4230 50%,#1e5038 100%);border-radius:10px 10px 0 0;padding:34px 30px;color:#fff;position:relative;overflow:hidden}\n.emb223-hero::after{content:'EMB';font-size:110px;font-weight:700;color:rgba(255,255,255,.04);position:absolute;right:0;bottom:-15px;pointer-events:none;font-family:monospace}\n.emb223-hero h1{margin:0 0 6px;font-size:25px;font-weight:800;color:#fff;position:relative}\n.emb223-hero h1 span{color:#86efac}\n.emb223-hero p{font-size:14px;color:#a7d8b8;margin:0 0 16px;max-width:720px;position:relative}\n.emb223-badges{display:flex;flex-wrap:wrap;gap:6px;position:relative}\n.emb223-badge{background:rgba(255,255,255,.11);border:1px solid rgba(255,255,255,.22);border-radius:20px;padding:4px 12px;font-size:11px;color:#fff;font-weight:600}\n.emb223-badge.green{background:rgba(72,200,100,.2);border-color:#48c864;color:#b8f0c8}\n.emb223-badge.amber{background:rgba(200,160,50,.2);border-color:#c8a032;color:#f0d880}\n.emb223-badge.purple{background:rgba(160,80,200,.2);border-color:#a050c8;color:#e0b8f8}\n.emb223-badge.red{background:rgba(200,60,60,.2);border-color:#c04040;color:#f8b8b8}\n.emb223-badge.slate{background:rgba(148,163,184,.15);border-color:#94a3b8;color:#e2e8f0}\n\/* COLONY STRIP *\/\n.emb223-col-strip{display:grid;grid-template-columns:1fr 1fr 1fr;background:#0a1f10}\n.emb223-cs{padding:12px 14px;border-right:1px solid rgba(255,255,255,.1);text-align:center}\n.emb223-cs:last-child{border-right:none}\n.emb223-cs-dot{width:22px;height:22px;border-radius:50%;margin:0 auto 6px;border:2px solid rgba(255,255,255,.2)}\n.emb223-cs-name{font-size:12px;font-weight:700;color:#fff;margin-bottom:2px}\n.emb223-cs-col{font-size:11px;color:rgba(255,255,255,.6)}\n\/* SPEC STRIP *\/\n.emb223-strip{display:grid;grid-template-columns:repeat(5,1fr);background:#f0f8f2;border-left:1px solid #a8d8b8;border-right:1px solid #a8d8b8}\n.emb223-si{padding:10px 13px;border-right:1px solid #a8d8b8;border-bottom:3px solid #1a4230}\n.emb223-si:last-child{border-right:none}\n.emb223-sl{font-size:9px;font-weight:700;color:#1a4230;letter-spacing:.1em;text-transform:uppercase;margin-bottom:2px}\n.emb223-sv{font-size:13px;font-weight:700;color:#1f2937}\n\/* TRUST BAR *\/\n.emb223-trust{display:grid;grid-template-columns:repeat(4,1fr);background:#0f2a1a}\n.emb223-ti{padding:9px 13px;border-right:1px solid rgba(255,255,255,.15);font-size:11px;color:#fff}\n.emb223-ti:last-child{border-right:none}\n.emb223-ti strong{display:block;font-size:11px;color:#86efac;margin-bottom:1px}\n\/* WARN BANNER *\/\n.emb223-warn-banner{background:#7a4a10;border-bottom:3px solid #8a5818;padding:11px 20px;display:flex;align-items:center;gap:10px}\n.emb223-warn-banner strong{font-size:13px;color:#fff}\n.emb223-warn-banner span{font-size:12px;color:#f8d8a8}\n\/* TABS CSS-only Shopify compatible *\/\n.emb223-radio{display:none}\n.emb223-tabrow{display:flex;flex-wrap:wrap;gap:3px;border-bottom:2px solid #a8d8b8;background:#c8e8d0;padding:7px 7px 0}\n.emb223-tabrow label{background:#a8d8b8;border:1px solid #80b898;border-bottom:none;border-radius:5px 5px 0 0;padding:8px 15px;font-size:13px;font-weight:600;color:#0f2a1a;cursor:pointer;user-select:none;transition:background .15s}\n.emb223-tabrow label:hover{background:#80b898}\n.emb223-panels .emb223-panel{display:none;background:#fff;border:1px solid #a8d8b8;border-top:none;border-radius:0 0 8px 8px;padding:26px 22px}\n#em1:checked~.emb223-tabwrap .emb223-panels #emp1,\n#em2:checked~.emb223-tabwrap .emb223-panels #emp2,\n#em3:checked~.emb223-tabwrap .emb223-panels #emp3,\n#em4:checked~.emb223-tabwrap .emb223-panels #emp4{display:block}\n#em1:checked~.emb223-tabwrap .emb223-tabrow label[for=em1],\n#em2:checked~.emb223-tabwrap .emb223-tabrow label[for=em2],\n#em3:checked~.emb223-tabwrap .emb223-tabrow label[for=em3],\n#em4:checked~.emb223-tabwrap .emb223-tabrow label[for=em4]{background:#fff;color:#1a4230;border-bottom:2px solid #fff;margin-bottom:-2px;font-weight:700}\n\/* SECTION HEADERS *\/\n.emb223-sh{background:#1a4230;color:#fff;padding:7px 14px;border-radius:4px;font-size:13px;font-weight:700;margin:22px 0 10px;letter-spacing:.03em}\n.emb223-sh:first-child{margin-top:0}\n\/* TABLES *\/\n.emb223-tbl{width:100%;border-collapse:collapse;font-size:14px;margin-bottom:14px}\n.emb223-tbl th{background:#c8e8d0;color:#0f2a1a;font-weight:700;padding:9px 11px;text-align:left;border-bottom:2px solid #a8d8b8}\n.emb223-tbl td{padding:8px 11px;border-bottom:1px solid #f0f8f2;color:#374151;vertical-align:top}\n.emb223-tbl tr:last-child td{border-bottom:none}\n.emb223-tbl tr:hover td{background:#f4fcf6}\n.emb223-tbl td:first-child{font-weight:600;color:#0f2a1a}\n.emb223-tbl .hi{background:#f0f8f2}\n.emb223-tbl .key{color:#1a4230;font-weight:600}\n.emb223-tbl .inh{color:#7a1a1a;font-weight:600}\n.emb223-tbl .sheen{color:#155724;font-weight:700}\n.emb223-tbl .purple-col{color:#5b21b6;font-weight:700}\n.emb223-formula-total td{background:#0f2a1a!important;color:#86efac!important;font-weight:700!important;padding:10px 11px!important;border:none!important}\n.emb223-formula-total td:first-child{color:#fff!important}\n\/* INFO BOXES *\/\n.emb223-info{background:#f0f8f2;border-left:4px solid #1a4230;border-radius:0 5px 5px 0;padding:12px 16px;margin:12px 0;font-size:14px;color:#1f2937}\n.emb223-green{background:#f0f6f2;border-left:4px solid #2a5240;border-radius:0 5px 5px 0;padding:12px 16px;margin:12px 0;font-size:14px;color:#1f2937}\n.emb223-warn{background:#faf6ee;border-left:4px solid #8a6820;border-radius:0 5px 5px 0;padding:11px 15px;font-size:13px;color:#5a4010;margin:10px 0}\n.emb223-danger{background:#fef2f2;border-left:4px solid #8a2020;border-radius:0 5px 5px 0;padding:12px 16px;font-size:13px;color:#7a1a1a;margin:10px 0}\n\/* COLONY RESULT CARDS *\/\n.emb223-cols{display:grid;grid-template-columns:1fr 1fr 1fr;gap:10px;margin:12px 0}\n.emb223-col-sheen{background:#d1fae5;border:2px solid #059669;border-radius:8px;padding:14px;text-align:center}\n.emb223-col-sheen h4{color:#065f46;font-size:13px;margin:0 0 5px}\n.emb223-col-sheen p{font-size:12px;color:#047857;margin:0}\n.emb223-col-pink{background:#ede9fe;border:2px solid #7c3aed;border-radius:8px;padding:14px;text-align:center}\n.emb223-col-pink h4{color:#4c1d95;font-size:13px;margin:0 0 5px}\n.emb223-col-pink p{font-size:12px;color:#5b21b6;margin:0}\n.emb223-col-none{background:#f3f4f6;border:2px solid #9ca3af;border-radius:8px;padding:14px;text-align:center}\n.emb223-col-none h4{color:#374151;font-size:13px;margin:0 0 5px}\n.emb223-col-none p{font-size:12px;color:#6b7280;margin:0}\n\/* MECHANISM PATHWAY *\/\n.emb223-path{background:#f0f8f2;border:1px solid #a8d8b8;border-radius:6px;padding:16px;font-size:13px;text-align:center;line-height:2.4;margin:12px 0;font-family:monospace;color:#0f2a1a}\n\/* APPS *\/\n.emb223-apps{display:grid;grid-template-columns:repeat(auto-fill,minmax(148px,1fr));gap:10px;margin:12px 0}\n.emb223-app{background:#f0f8f2;border:1px solid #a8d8b8;border-top:3px solid #1a4230;border-radius:6px;padding:12px;text-align:center}\n.emb223-app .ico{font-size:22px;margin-bottom:5px}\n.emb223-app .ct{font-size:13px;font-weight:600;color:#0f2a1a}\n.emb223-app .cs{font-size:11px;color:#6b7280;margin-top:2px}\n\/* PREP STEPS *\/\n.emb223-steps{margin:12px 0}\n.emb223-step{display:flex;gap:11px;margin-bottom:9px;align-items:flex-start}\n.emb223-stepn{background:#1a4230;color:#fff;font-weight:700;font-size:12px;min-width:26px;height:26px;border-radius:50%;display:flex;align-items:center;justify-content:center;flex-shrink:0}\n.emb223-stept{font-size:14px;color:#374151;padding-top:3px}\n\/* FAQ *\/\n.emb223-faq{border:1px solid #a8d8b8;border-radius:7px;overflow:hidden;margin:12px 0}\n.emb223-fq{border-bottom:1px solid #a8d8b8}\n.emb223-fq:last-child{border-bottom:none}\n.emb223-fqq{padding:11px 14px;font-size:14px;font-weight:600;color:#0f2a1a;background:#f0f8f2}\n.emb223-fqa{padding:0 14px 11px;font-size:14px;color:#374151}\n\/* RELATED *\/\n.emb223-related{display:grid;grid-template-columns:repeat(auto-fill,minmax(148px,1fr));gap:10px;margin:12px 0}\n.emb223-rp{background:#f0f8f2;border:1px solid #a8d8b8;border-radius:7px;padding:12px 10px;text-align:center;text-decoration:none;display:block;transition:box-shadow .2s}\n.emb223-rp:hover{box-shadow:0 3px 10px rgba(26,66,48,.18);border-color:#1a4230}\n.emb223-rp .rcat{font-size:10px;color:#94a3b8;font-weight:600;letter-spacing:.05em;text-transform:uppercase}\n.emb223-rp .rname{font-size:13px;font-weight:700;color:#0f2a1a;margin:3px 0}\n.emb223-rp .ruse{font-size:11px;color:#6b7280}\n\/* FOOTER *\/\n.emb223-footer{background:#f0f8f2;border-top:3px solid #0f2a1a;border-radius:0 0 8px 8px;padding:14px 20px;font-size:12px;color:#555}\n@media(max-width:600px){.emb223-hero{padding:20px 14px}.emb223-hero h1{font-size:20px}.emb223-col-strip{grid-template-columns:1fr}.emb223-strip{grid-template-columns:repeat(3,1fr)}.emb223-trust{grid-template-columns:repeat(2,1fr)}.emb223-panels .emb223-panel{padding:16px 11px}.emb223-tabrow label{padding:7px 9px;font-size:12px}.emb223-cols{grid-template-columns:1fr}}\n\u003c\/style\u003e\n\u003cdiv class=\"emb223\"\u003e\n\u003c!-- HERO --\u003e\n\u003cdiv class=\"emb223-hero\"\u003e\n\u003ch1\u003e\n\u003cspan\u003eEMB Agar\u003c\/span\u003e — Eosin Methylene Blue Agar\u003c\/h1\u003e\n\u003cp\u003eSelective \u0026amp; Differential Medium for Gram-Negative Enteric Bacteria | Metallic Green Sheen for \u003cem\u003eE. coli\u003c\/em\u003e | Cat. No. AS-1223\u003c\/p\u003e\n\u003cdiv class=\"emb223-badges\"\u003e\n\u003cspan class=\"emb223-badge\"\u003eCat. No. AS-1223\u003c\/span\u003e \u003cspan class=\"emb223-badge\"\u003epH 7.1 ± 0.2\u003c\/span\u003e \u003cspan class=\"emb223-badge\"\u003e~36 g\/L\u003c\/span\u003e \u003cspan class=\"emb223-badge red\"\u003e⚠ DO NOT OVERHEAT\u003c\/span\u003e \u003cspan class=\"emb223-badge green\"\u003e🟪 E. coli → Metallic Green Sheen\u003c\/span\u003e \u003cspan class=\"emb223-badge purple\"\u003e⬤ Coliforms → Blue-Black \/ Purple\u003c\/span\u003e \u003cspan class=\"emb223-badge slate\"\u003e⭘ Non-fermenters → Colourless\u003c\/span\u003e \u003cspan class=\"emb223-badge green\"\u003e✓ Sigma 70186 Equivalent\u003c\/span\u003e \u003cspan class=\"emb223-badge amber\"\u003e🇦🇺 Made in Melbourne\u003c\/span\u003e \u003cspan class=\"emb223-badge amber\"\u003e⚡ Same-Week Dispatch\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- COLONY COLOUR STRIP --\u003e\n\u003cdiv class=\"emb223-col-strip\"\u003e\n\u003cdiv class=\"emb223-cs\"\u003e\n\u003cdiv style=\"background: linear-gradient(135deg,#065f46,#10b981);\" class=\"emb223-cs-dot\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"emb223-cs-name\"\u003e\n\u003cem\u003eE. coli\u003c\/em\u003e — Strong Fermenters\u003c\/div\u003e\n\u003cdiv class=\"emb223-cs-col\"\u003eDark metallic green sheen\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-cs\"\u003e\n\u003cdiv style=\"background: linear-gradient(135deg,#312e81,#7c3aed);\" class=\"emb223-cs-dot\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"emb223-cs-name\"\u003eOther Coliforms \/ Fermenters\u003c\/div\u003e\n\u003cdiv class=\"emb223-cs-col\"\u003eBlue-black to purple (mucoid)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-cs\"\u003e\n\u003cdiv style=\"background: #d1d5db; border-color: #9ca3af;\" class=\"emb223-cs-dot\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"emb223-cs-name\"\u003eNon-fermenters (Salmonella etc.)\u003c\/div\u003e\n\u003cdiv class=\"emb223-cs-col\"\u003eColourless or translucent\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- WARN BANNER --\u003e\n\u003cdiv class=\"emb223-warn-banner\"\u003e\n\u003cstrong\u003e⚠️ CRITICAL — DO NOT OVERHEAT:\u003c\/strong\u003e \u003cspan\u003eExcess heat degrades dyes and impairs differentiation. Swirl gently before pouring to re-oxidise methylene blue and restore blue colour. See Tab 2 for full protocol.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c!-- SPEC STRIP --\u003e\n\u003cdiv class=\"emb223-strip\"\u003e\n\u003cdiv class=\"emb223-si\"\u003e\n\u003cdiv class=\"emb223-sl\"\u003eCat. No.\u003c\/div\u003e\n\u003cdiv class=\"emb223-sv\"\u003eAS-1223\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-si\"\u003e\n\u003cdiv class=\"emb223-sl\"\u003epH (25°C)\u003c\/div\u003e\n\u003cdiv class=\"emb223-sv\"\u003e7.1 ± 0.2\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-si\"\u003e\n\u003cdiv class=\"emb223-sl\"\u003eDissolution\u003c\/div\u003e\n\u003cdiv class=\"emb223-sv\"\u003e~36 g\/L\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-si\"\u003e\n\u003cdiv class=\"emb223-sl\"\u003eAutoclave\u003c\/div\u003e\n\u003cdiv class=\"emb223-sv\"\u003e121°C \/ 15 min\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-si\"\u003e\n\u003cdiv class=\"emb223-sl\"\u003eIncubation\u003c\/div\u003e\n\u003cdiv class=\"emb223-sv\"\u003e35°C \/ 24–48h\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- TRUST BAR --\u003e\n\u003cdiv class=\"emb223-trust\"\u003e\n\u003cdiv class=\"emb223-ti\"\u003e\n\u003cstrong\u003e📄 Full Documentation\u003c\/strong\u003eCOA, TDS \u0026amp; SDS every batch\u003c\/div\u003e\n\u003cdiv class=\"emb223-ti\"\u003e\n\u003cstrong\u003e🇦🇺 Australian Stock\u003c\/strong\u003eNo import delays\u003c\/div\u003e\n\u003cdiv class=\"emb223-ti\"\u003e\n\u003cstrong\u003e⚡ Same-Week Dispatch\u003c\/strong\u003eMelbourne warehouse\u003c\/div\u003e\n\u003cdiv class=\"emb223-ti\"\u003e\n\u003cstrong\u003e🔬 Technical Support\u003c\/strong\u003eDirect from our team\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- TABS CSS-only radio — Shopify compatible --\u003e \u003cinput checked id=\"em1\" name=\"emb223tabs\" type=\"radio\" class=\"emb223-radio\"\u003e \u003cinput id=\"em2\" name=\"emb223tabs\" type=\"radio\" class=\"emb223-radio\"\u003e \u003cinput id=\"em3\" name=\"emb223tabs\" type=\"radio\" class=\"emb223-radio\"\u003e \u003cinput id=\"em4\" name=\"emb223tabs\" type=\"radio\" class=\"emb223-radio\"\u003e\n\u003cdiv class=\"emb223-tabwrap\"\u003e\n\u003cdiv class=\"emb223-tabrow\"\u003e\n\u003clabel for=\"em1\"\u003eProduct Description\u003c\/label\u003e \u003clabel for=\"em2\"\u003eTechnical Data\u003c\/label\u003e \u003clabel for=\"em3\"\u003eSafety Data\u003c\/label\u003e \u003clabel for=\"em4\"\u003eQuality \u0026amp; COA\u003c\/label\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-panels\"\u003e\n\u003c!-- TAB 1: PRODUCT DESCRIPTION --\u003e\n\u003cdiv class=\"emb223-panel\" id=\"emp1\"\u003e\n\u003cdiv class=\"emb223-sh\"\u003eOverview\u003c\/div\u003e\n\u003cp style=\"font-size: 14px; color: #374151; margin: 0 0 11px;\"\u003e\u003cstrong\u003eEMB Agar (AS-1223)\u003c\/strong\u003e is a selective and differential solid culture medium for the isolation and differentiation of Gram-negative enteric bacteria, particularly coliform organisms including \u003cem\u003eEscherichia coli\u003c\/em\u003e. Originally formulated by Holt-Harris and Teague (1916) and later modified by Levine (1918), AS-1223 uses a combination of both classical formulations — retaining \u003cstrong\u003elactose and sucrose\u003c\/strong\u003e as dual fermentable carbohydrates and the characteristic \u003cstrong\u003eEosin Y \/ Methylene Blue\u003c\/strong\u003e dual dye indicator system.\u003c\/p\u003e\n\u003cp style=\"font-size: 14px; color: #374151; margin: 0 0 11px;\"\u003eThe unique feature of EMB Agar is the \u003cstrong\u003emetallic green sheen\u003c\/strong\u003e produced by strong lactose fermenters such as \u003cem\u003eE. coli\u003c\/em\u003e — a visual endpoint requiring no further confirmation in many routine applications. This sheen results from the precipitation of the eosin-methylene blue complex under strongly acidic conditions produced by vigorous lactose fermentation.\u003c\/p\u003e\n\u003cdiv class=\"emb223-sh\"\u003eColony Colour Interpretation\u003c\/div\u003e\n\u003cdiv class=\"emb223-cols\"\u003e\n\u003cdiv class=\"emb223-col-sheen\"\u003e\n\u003ch4\u003e🟪 METALLIC GREEN SHEEN\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cem\u003eE. coli\u003c\/em\u003e\u003c\/strong\u003e — strong lactose fermenter\u003cbr\u003eVigorous acid production\u003cbr\u003eEosin-MB complex precipitates\u003cbr\u003eat very low pH\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-col-pink\"\u003e\n\u003ch4\u003e⬤ BLUE-BLACK \/ PURPLE\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003eOther coliforms\u003c\/strong\u003e\u003cbr\u003e\u003cem\u003eEnterobacter, Klebsiella\u003c\/em\u003e\u003cbr\u003eModerate fermenters\u003cbr\u003eMucoid colonies\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-col-none\"\u003e\n\u003ch4\u003e⭘ COLOURLESS \/ TRANSLUCENT\u003c\/h4\u003e\n\u003cp\u003e\u003cstrong\u003eNon-fermenters\u003c\/strong\u003e\u003cbr\u003e\u003cem\u003eSalmonella, Shigella,\u003c\/em\u003e\u003cbr\u003e\u003cem\u003ePseudomonas\u003c\/em\u003e\u003cbr\u003eNo acid — no dye reaction\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-warn\"\u003e⚠️ \u003cstrong\u003eConfirmation required:\u003c\/strong\u003e Metallic green sheen is presumptive for \u003cem\u003eE. coli\u003c\/em\u003e — not confirmed. All presumptive positives should be confirmed by standard biochemical tests (IMViC, TSI, oxidase) per FDA BAM or equivalent validated method. Some \u003cem\u003eCitrobacter\u003c\/em\u003e and \u003cem\u003eEnterobacter\u003c\/em\u003e strains may produce partial sheen.\u003c\/div\u003e\n\u003cdiv class=\"emb223-sh\"\u003eDetection Mechanism — Dual Dye System\u003c\/div\u003e\n\u003cdiv class=\"emb223-info\"\u003e\n\u003cstrong\u003eMethylene Blue (0.065 g\/L) — Selective inhibitor:\u003c\/strong\u003e Inhibits the growth of most Gram-positive organisms by interfering with their cell membrane. Gram-negative bacteria are largely unaffected at this concentration, providing selectivity in mixed-flora samples.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eEosin Y (0.4 g\/L) — pH indicator + inhibitor:\u003c\/strong\u003e At neutral pH, Eosin Y is soluble and produces pink\/amber coloration. As organisms ferment carbohydrates and produce acid, Eosin Y combines with Methylene Blue to form an insoluble complex that precipitates onto colonies — producing the purple, blue-black, or characteristic metallic green sheen depending on the degree of acid production.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eThe metallic green sheen mechanism:\u003c\/strong\u003e In strong acid producers such as \u003cem\u003eE. coli\u003c\/em\u003e, the pH around the colony drops sharply. The dye complex precipitates in such large amounts that it forms a reflective layer on the colony surface, producing the iridescent metallic green appearance when viewed under oblique reflected light.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eDipotassium Phosphate (2.0 g\/L):\u003c\/strong\u003e Buffers the medium to maintain pH 7.1 ± 0.2 — essential for consistent dye behaviour and reproducible colony differentiation.\u003c\/div\u003e\n\u003cdiv class=\"emb223-sh\"\u003eDetection Pathway\u003c\/div\u003e\n\u003cdiv class=\"emb223-path\"\u003eStrong acid production (E. coli) → pH drops sharply around colony\u003cbr\u003e↓\u003cbr\u003eEosin Y + Methylene Blue → insoluble complex precipitates on colony surface\u003cbr\u003e↓\u003cbr\u003eVery heavy precipitation at very low pH → \u003cstrong\u003e🟪 Metallic green sheen\u003c\/strong\u003e\u003cbr\u003e――――――――――――――――――――\u003cbr\u003eModerate acid (Klebsiella, Enterobacter) → moderate precipitation → \u003cstrong\u003e⬤ Blue-black \/ purple\u003c\/strong\u003e\u003cbr\u003e――――――――――――――――――――\u003cbr\u003eNo fermentation (Salmonella, Shigella) → no acid → no dye precipitation → \u003cstrong\u003e⭘ Colourless\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-sh\"\u003eWhy Two Carbohydrates?\u003c\/div\u003e\n\u003cdiv class=\"emb223-info\"\u003e\n\u003cstrong\u003eLactose (5.0 g\/L):\u003c\/strong\u003e Primary fermentable carbohydrate. Differentiates lactose fermenters (coliforms) from non-fermenters (Salmonella, Shigella). The original Levine formula used lactose alone at 10 g\/L.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSucrose (5.0 g\/L):\u003c\/strong\u003e Added per the Holt-Harris and Teague formulation. Some organisms ferment sucrose more readily than lactose — notably \u003cem\u003eProteus\u003c\/em\u003e species, which are sucrose-positive but lactose-negative. The presence of sucrose enables differentiation of sucrose-positive non-lactose-fermenters from pathogens such as \u003cem\u003eSalmonella\u003c\/em\u003e and \u003cem\u003eShigella\u003c\/em\u003e, which ferment neither carbohydrate and remain colourless.\u003c\/div\u003e\n\u003cdiv class=\"emb223-sh\"\u003eApplications\u003c\/div\u003e\n\u003cdiv class=\"emb223-apps\"\u003e\n\u003cdiv class=\"emb223-app\"\u003e\n\u003cdiv class=\"ico\"\u003e💧\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eWater Quality\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eColiform detection, E. coli confirmation in water samples\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-app\"\u003e\n\u003cdiv class=\"ico\"\u003e🍕\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eFood Safety\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eEnteric indicator screening in food and beverages\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-app\"\u003e\n\u003cdiv class=\"ico\"\u003e🧬\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eClinical \/ Medical\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eEnteric pathogen isolation from faecal and clinical specimens\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-app\"\u003e\n\u003cdiv class=\"ico\"\u003e🏭\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eEnvironmental\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eSurface swab monitoring, hygiene testing, sanitation QC\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-app\"\u003e\n\u003cdiv class=\"ico\"\u003e💊\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003ePharmaceutical QC\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eEnteric contamination detection in pharma water and products\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-app\"\u003e\n\u003cdiv class=\"ico\"\u003e📚\u003c\/div\u003e\n\u003cdiv class=\"ct\"\u003eTeaching Labs\u003c\/div\u003e\n\u003cdiv class=\"cs\"\u003eSelective\/differential media demonstration, fermentation patterns\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-sh\"\u003eEMB Agar vs Related Media\u003c\/div\u003e\n\u003ctable class=\"emb223-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eMedium\u003c\/th\u003e\n\u003cth\u003eTarget\u003c\/th\u003e\n\u003cth\u003eE. coli Result\u003c\/th\u003e\n\u003cth\u003eKey Feature\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003e\u003cstrong\u003eEMB Agar (AS-1223) ★\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eGram-neg enteric bacteria\u003c\/td\u003e\n\u003ctd class=\"sheen\"\u003e🟪 Metallic green sheen\u003c\/td\u003e\n\u003ctd\u003eDual carbohydrate — Lactose + Sucrose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMacConkey Agar\u003c\/td\u003e\n\u003ctd\u003eGram-neg \/ coliforms\u003c\/td\u003e\n\u003ctd style=\"color: #dc2626; font-weight: 600;\"\u003e🔴 Pink \/ red colonies\u003c\/td\u003e\n\u003ctd\u003eNeutral red indicator; bile salts\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eTBX Agar\u003c\/td\u003e\n\u003ctd\u003e\n\u003cem\u003eE. coli\u003c\/em\u003e specific\u003c\/td\u003e\n\u003ctd style=\"color: #15803d; font-weight: 600;\"\u003e🟢 Blue-green (β-gur)\u003c\/td\u003e\n\u003ctd\u003eChromogenic β-glucuronidase substrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eECC Agar (AS-1438)\u003c\/td\u003e\n\u003ctd\u003e\n\u003cem\u003eE. coli\u003c\/em\u003e + coliforms\u003c\/td\u003e\n\u003ctd style=\"color: #1e3a8a; font-weight: 600;\"\u003e🔵 Dark blue-violet\u003c\/td\u003e\n\u003ctd\u003eDual chromogenic — ISO 9308-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eEndo Agar\u003c\/td\u003e\n\u003ctd\u003eTotal coliforms\u003c\/td\u003e\n\u003ctd style=\"color: #dc2626; font-weight: 600;\"\u003eRed + golden sheen\u003c\/td\u003e\n\u003ctd\u003eBasic fuchsin indicator; no sucrose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"emb223-sh\"\u003eFAQs\u003c\/div\u003e\n\u003cdiv class=\"emb223-faq\"\u003e\n\u003cdiv class=\"emb223-fq\"\u003e\n\u003cdiv class=\"emb223-fqq\"\u003e❓ Why is the metallic sheen only seen under reflected light?\u003c\/div\u003e\n\u003cdiv class=\"emb223-fqa\"\u003eThe metallic green sheen is an optical phenomenon caused by the crystalline precipitation of the eosin-methylene blue complex on the colony surface. This layer acts as a thin film that reflects light at a specific angle. To observe the sheen correctly, hold the plate at an angle to a strong light source and look at reflected — not transmitted — light. Looking through the plate (transmitted light) may show dark colonies but not the characteristic metallic sheen.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-fq\"\u003e\n\u003cdiv class=\"emb223-fqq\"\u003e❓ Why must I swirl the medium before pouring?\u003c\/div\u003e\n\u003cdiv class=\"emb223-fqa\"\u003eMethylene blue is partially reduced (decolourised) to its leuco form during autoclaving — the medium may appear lighter than expected post-sterilisation. Swirling gently while cooling to 45–50°C re-oxidises the dye in contact with air, restoring the characteristic dark blue-purple colour of prepared EMB Agar. Plates poured without this step may produce inconsistent colony colour results due to uneven dye oxidation. Do not shake vigorously — only gentle swirling is needed.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-fq\"\u003e\n\u003cdiv class=\"emb223-fqq\"\u003e❓ What is the difference between AS-1223 and Levine EMB (Sigma 70186)?\u003c\/div\u003e\n\u003cdiv class=\"emb223-fqa\"\u003eSigma 70186 (Levine's formula) uses only \u003cstrong\u003elactose (10 g\/L)\u003c\/strong\u003e with no sucrose. AS-1223 uses the combined Holt-Harris \u0026amp; Teague + Levine formulation with \u003cstrong\u003elactose (5 g\/L) + sucrose (5 g\/L)\u003c\/strong\u003e. The combined formula adds the ability to differentiate sucrose-fermenting organisms (e.g. \u003cem\u003eProteus\u003c\/em\u003e) from non-fermenters (Salmonella, Shigella). For routine E. coli detection, both perform equivalently. For broader Enterobacteriaceae differentiation, the dual carbohydrate formula provides more information.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-fq\"\u003e\n\u003cdiv class=\"emb223-fqq\"\u003e❓ Do all E. coli strains produce metallic sheen?\u003c\/div\u003e\n\u003cdiv class=\"emb223-fqa\"\u003eMost — but not all. Some \u003cem\u003eE. coli\u003c\/em\u003e strains are weak acid producers and may produce dark purple colonies rather than metallic sheen. \u003cem\u003eE. coli\u003c\/em\u003e O157:H7 is a notable example that may not produce a typical metallic sheen on EMB Agar. Always confirm presumptive positives by standard biochemical testing. Conversely, some \u003cem\u003eCitrobacter freundii\u003c\/em\u003e and \u003cem\u003eEnterobacter\u003c\/em\u003e strains may produce a partial sheen, which should not be confused with a positive \u003cem\u003eE. coli\u003c\/em\u003e result.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-sh\"\u003eCross-Reference \/ Equivalents\u003c\/div\u003e\n\u003ctable class=\"emb223-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eManufacturer\u003c\/th\u003e\n\u003cth\u003eProduct Name\u003c\/th\u003e\n\u003cth\u003eCat. No.\u003c\/th\u003e\n\u003cth\u003eFormula\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSigma-Aldrich \/ Merck\u003c\/td\u003e\n\u003ctd\u003eEMB Agar (Levine)\u003c\/td\u003e\n\u003ctd\u003e70186\u003c\/td\u003e\n\u003ctd\u003eLactose only (10 g\/L)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBD Difco\u003c\/td\u003e\n\u003ctd\u003eLevine EMB Agar\u003c\/td\u003e\n\u003ctd\u003e221010\u003c\/td\u003e\n\u003ctd\u003eLactose only (10 g\/L)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHiMedia\u003c\/td\u003e\n\u003ctd\u003eEMB Agar (Holt-Harris + Levine)\u003c\/td\u003e\n\u003ctd\u003eM023\u003c\/td\u003e\n\u003ctd\u003eLactose 5g + Sucrose 5g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermo Scientific \/ Oxoid\u003c\/td\u003e\n\u003ctd\u003eEosin Methylene Blue Agar\u003c\/td\u003e\n\u003ctd\u003eCM0069\u003c\/td\u003e\n\u003ctd\u003eLactose + Sucrose\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"emb223-sh\"\u003eRelated Products\u003c\/div\u003e\n\u003cdiv class=\"emb223-related\"\u003e\n\u003ca href=\"https:\/\/ausamics.com.au\/products\/ecc-agar\" class=\"emb223-rp\" target=\"_blank\"\u003e\n\u003cdiv class=\"rcat\"\u003eAS-1438\u003c\/div\u003e\n\u003cdiv class=\"rname\"\u003eECC Agar\u003c\/div\u003e\n\u003cdiv class=\"ruse\"\u003eE. coli + coliforms — ISO 9308-1\u003c\/div\u003e\n\u003c\/a\u003e\u003ca href=\"https:\/\/ausamics.com.au\/products\/macconkey-agar\" class=\"emb223-rp\" target=\"_blank\"\u003e\n\u003cdiv class=\"rcat\"\u003eAS-1278\u003c\/div\u003e\n\u003cdiv class=\"rname\"\u003eMacConkey Agar\u003c\/div\u003e\n\u003cdiv class=\"ruse\"\u003eGram-neg selection + coliforms\u003c\/div\u003e\n\u003c\/a\u003e\u003ca href=\"https:\/\/ausamics.com.au\/products\/tryptic-soy-agar\" class=\"emb223-rp\" target=\"_blank\"\u003e\n\u003cdiv class=\"rcat\"\u003eAS-1370\u003c\/div\u003e\n\u003cdiv class=\"rname\"\u003eTryptic Soy Agar\u003c\/div\u003e\n\u003cdiv class=\"ruse\"\u003eGeneral cultivation \/ subculture\u003c\/div\u003e\n\u003c\/a\u003e\u003ca href=\"https:\/\/ausamics.com.au\/products\/xld-agar\" class=\"emb223-rp\" target=\"_blank\"\u003e\n\u003cdiv class=\"rcat\"\u003eAS-1380\u003c\/div\u003e\n\u003cdiv class=\"rname\"\u003eXLD Agar\u003c\/div\u003e\n\u003cdiv class=\"ruse\"\u003eSalmonella \/ Shigella isolation\u003c\/div\u003e\n\u003c\/a\u003e\u003ca href=\"https:\/\/ausamics.com.au\/collections\/culture-media\" class=\"emb223-rp\" target=\"_blank\"\u003e\n\u003cdiv class=\"rcat\"\u003eVIEW ALL\u003c\/div\u003e\n\u003cdiv class=\"rname\"\u003eCulture Media\u003c\/div\u003e\n\u003cdiv class=\"ruse\"\u003eFull catalogue →\u003c\/div\u003e\n\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- \/tab1 --\u003e \u003c!-- TAB 2: TECHNICAL DATA --\u003e\n\u003cdiv class=\"emb223-panel\" id=\"emp2\"\u003e\n\u003cdiv class=\"emb223-sh\"\u003eProduct Specifications\u003c\/div\u003e\n\u003ctable class=\"emb223-tbl\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eEMB Agar — Eosin Methylene Blue Agar\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalogue Number\u003c\/td\u003e\n\u003ctd\u003eAS-1223\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSynonyms\u003c\/td\u003e\n\u003ctd\u003eEMB Agar; Eosin Methylene Blue Agar; Levine EMB Agar; E.M.B. Medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormulation Type\u003c\/td\u003e\n\u003ctd\u003eCombined Holt-Harris \u0026amp; Teague (1916) + Levine (1918) formulation — dual carbohydrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEquivalents\u003c\/td\u003e\n\u003ctd\u003eSigma-Aldrich 70186 (Levine) | BD Difco 221010 | HiMedia M023 | Oxoid CM0069\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMedium Type\u003c\/td\u003e\n\u003ctd\u003eSelective and differential solid agar\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDissolution\u003c\/td\u003e\n\u003ctd\u003e~36 g\/L in purified or distilled water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFinal pH at 25°C\u003c\/td\u003e\n\u003ctd\u003e7.1 ± 0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSterilisation\u003c\/td\u003e\n\u003ctd\u003eAutoclave 121°C for 15 minutes. \u003cstrong\u003eDO NOT OVERHEAT\u003c\/strong\u003e — degrades dyes and impairs differentiation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (powder)\u003c\/td\u003e\n\u003ctd\u003eFaint violet to pink, homogeneous, hygroscopic powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (prepared)\u003c\/td\u003e\n\u003ctd\u003eDeep red-brown, clear to slightly turbid; firm gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePost-autoclave — Critical\u003c\/td\u003e\n\u003ctd\u003eSwirl gently at 45–50°C to re-oxidise methylene blue (restore blue colour) before pouring\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIncubation\u003c\/td\u003e\n\u003ctd\u003e35°C for 24–48 hours; aerobically\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage (powder)\u003c\/td\u003e\n\u003ctd\u003e15–30°C, dry, tightly sealed, protected from direct light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage (prepared plates)\u003c\/td\u003e\n\u003ctd\u003e2–8°C, protected from drying and direct light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHS Tariff Code\u003c\/td\u003e\n\u003ctd\u003e3821.00.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"emb223-sh\"\u003eFormula — Per Litre (Combined Holt-Harris \u0026amp; Teague + Levine)\u003c\/div\u003e\n\u003ctable class=\"emb223-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredient\u003c\/th\u003e\n\u003cth\u003eg \/ L\u003c\/th\u003e\n\u003cth\u003eRole\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003ePeptone (Enzymatic Digest of Animal Tissue)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e10.0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003ePrimary nitrogen source — amino acids, vitamins, carbon, growth factors\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLactose\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e5.0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"key\"\u003eFermentable carbohydrate — primary differential substrate for coliform detection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eSucrose\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e5.0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"key\"\u003eSecondary fermentable carbohydrate — differentiates sucrose-fermenting non-coliforms (e.g. Proteus) from Salmonella \/ Shigella\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDipotassium Hydrogen Phosphate (K₂HPO₄)\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e2.0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003epH buffer — maintains stable pH 7.1 ± 0.2; essential for consistent dye behaviour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003e\u003cstrong\u003eEosin Y\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.4\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAcid-sensitive pH indicator dye — combines with methylene blue under acid conditions to produce blue-black to metallic green precipitate on fermenting colonies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eMethylene Blue\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e0.065\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd class=\"inh\"\u003eSelective inhibitor — inhibits Gram-positive organisms. Also part of the dye complex that precipitates under acid conditions to produce colony colour reactions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e13.5\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eSolidifying agent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ctable style=\"margin-top: 2px;\" class=\"emb223-tbl\"\u003e\n\u003ctfoot\u003e\n\u003ctr class=\"emb223-formula-total\"\u003e\n\u003ctd\u003eTotal dissolution\u003c\/td\u003e\n\u003ctd\u003e~36 g\/L\u003c\/td\u003e\n\u003ctd\u003epH 7.1 ± 0.2 | Autoclave 121°C \/ 15 min | DO NOT OVERHEAT | Swirl before pouring\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tfoot\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"emb223-sh\"\u003ePreparation Protocol\u003c\/div\u003e\n\u003cdiv class=\"emb223-danger\"\u003e⚠️ \u003cstrong\u003eDO NOT OVERHEAT.\u003c\/strong\u003e Excessive autoclaving or prolonged boiling degrades both Eosin Y and Methylene Blue, reducing differentiation power and metallic sheen intensity. Use exactly 121°C \/ 15 minutes — no longer.\u003c\/div\u003e\n\u003cdiv class=\"emb223-steps\"\u003e\n\u003cdiv class=\"emb223-step\"\u003e\n\u003cdiv class=\"emb223-stepn\"\u003e1\u003c\/div\u003e\n\u003cdiv class=\"emb223-stept\"\u003eSuspend \u003cstrong\u003e~36 g\u003c\/strong\u003e of dehydrated EMB Agar (AS-1223) in \u003cstrong\u003e1 litre of purified or distilled water\u003c\/strong\u003e. Mix well.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-step\"\u003e\n\u003cdiv class=\"emb223-stepn\"\u003e2\u003c\/div\u003e\n\u003cdiv class=\"emb223-stept\"\u003eHeat with frequent agitation and \u003cstrong\u003ebring to boil\u003c\/strong\u003e to dissolve completely. Do not continue boiling after dissolution.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-step\"\u003e\n\u003cdiv class=\"emb223-stepn\"\u003e3\u003c\/div\u003e\n\u003cdiv class=\"emb223-stept\"\u003eSterilise by autoclaving at \u003cstrong\u003e121°C for exactly 15 minutes\u003c\/strong\u003e. Do not exceed this cycle.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-step\"\u003e\n\u003cdiv class=\"emb223-stepn\"\u003e4\u003c\/div\u003e\n\u003cdiv class=\"emb223-stept\"\u003eCool to \u003cstrong\u003e45–50°C\u003c\/strong\u003e in a water bath. \u003cstrong\u003eSwirl gently\u003c\/strong\u003e to re-oxidise methylene blue — restore blue colour before pouring. Do not shake vigorously.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-step\"\u003e\n\u003cdiv class=\"emb223-stepn\"\u003e5\u003c\/div\u003e\n\u003cdiv class=\"emb223-stept\"\u003ePour \u003cstrong\u003e15–20 mL per 90 mm Petri dish\u003c\/strong\u003e on a level surface. Allow to solidify. Dry briefly before use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-step\"\u003e\n\u003cdiv class=\"emb223-stepn\"\u003e6\u003c\/div\u003e\n\u003cdiv class=\"emb223-stept\"\u003eIncubate inoculated plates at \u003cstrong\u003e35°C for 24–48 hours\u003c\/strong\u003e. Read results under reflected light for metallic sheen detection.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-warn\"\u003e⚠️ \u003cstrong\u003eMetallic sheen reading:\u003c\/strong\u003e Always observe plates under \u003cstrong\u003eoblique reflected light\u003c\/strong\u003e (angled to a direct light source). Looking through the plate (transmitted light) will not reveal the metallic sheen.\u003c\/div\u003e\n\u003cdiv class=\"emb223-sh\"\u003eLiterature \u0026amp; References\u003c\/div\u003e\n\u003ctable class=\"emb223-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003e#\u003c\/th\u003e\n\u003cth\u003eReference\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003eHolt-Harris JE, Teague O. A new culture medium for the isolation of Bacillus typhosa from stools. J Infect Dis. 1916;18:596–600. \u003cstrong\u003e[Original EMB formulation]\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003eLevine M. The differentiation of B. coli and B. aerogenes on a simplified eosin-methylene-blue agar. J Infect Dis. 1918;23:43–47. \u003cstrong\u003e[Levine modification — Lactose only version]\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003ctd\u003eSigma-Aldrich \/ Merck. EMB Agar (Eosin Methylene Blue Agar), Cat. 70186. Product specification. \u003cstrong\u003e[Reference for Levine formula — Levine single-lactose version]\u003c\/strong\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e4\u003c\/td\u003e\n\u003ctd\u003eAPHA. Standard Methods for the Examination of Water and Wastewater. Current edition. American Public Health Association. [EMB reference for water microbiology]\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5\u003c\/td\u003e\n\u003ctd\u003eUS FDA. Bacteriological Analytical Manual (BAM). FDA; current edition. fda.gov\/food\/fda-food-safety-program\/bacteriological-analytical-manual-bam. [E. coli confirmation methods]\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e6\u003c\/td\u003e\n\u003ctd\u003eISO 7218:2007. Microbiology of food and animal feeding stuffs — General requirements and guidance for microbiological examinations. Geneva: ISO; 2007.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c!-- \/tab2 --\u003e \u003c!-- TAB 3: SAFETY DATA --\u003e\n\u003cdiv class=\"emb223-panel\" id=\"emp3\"\u003e\n\u003cdiv class=\"emb223-green\"\u003e📄 \u003cstrong\u003eFull 16-section GHS SDS available\u003c\/strong\u003e (Australian WHS Regulations 2023 \/ GHS 7th Edition) — \u003ca style=\"color: #2a5240;\" href=\"mailto:support@ausamics.com.au\"\u003esupport@ausamics.com.au\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"emb223-sh\"\u003eSection 1 — Identification\u003c\/div\u003e\n\u003ctable class=\"emb223-tbl\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Name\u003c\/td\u003e\n\u003ctd\u003eEMB Agar — Eosin Methylene Blue Agar\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalogue No.\u003c\/td\u003e\n\u003ctd\u003eAS-1223\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended Use\u003c\/td\u003e\n\u003ctd\u003eSelective and differential solid culture medium for isolation and differentiation of Gram-negative enteric bacteria. Laboratory, research, quality control, and educational use only.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSupplier\u003c\/td\u003e\n\u003ctd\u003eAuSaMicS Pty Ltd | ABN 56 676 640 467\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAddress\u003c\/td\u003e\n\u003ctd\u003e31 Longview CT, Thomastown VIC 3074, Australia\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEmergency\u003c\/td\u003e\n\u003ctd\u003ePoisons Information Centre: \u003cstrong\u003e13 11 26\u003c\/strong\u003e (24 hr)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhone\u003c\/td\u003e\n\u003ctd\u003e+61 412 520 598 | support@ausamics.com.au\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"emb223-sh\"\u003eSection 2 — Hazard Identification (GHS 7th Ed \/ WHS 2023)\u003c\/div\u003e\n\u003ctable class=\"emb223-tbl\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGHS Classification (powder)\u003c\/td\u003e\n\u003ctd\u003eNOT classified as hazardous under Australian WHS Regulations 2023 at normal use concentrations. Methylene blue at 0.065 g\/L in prepared medium does not reach hazardous classification threshold.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSignal Word\u003c\/td\u003e\n\u003ctd\u003eWarning (precautionary for dust during bulk weighing)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOther Hazards\u003c\/td\u003e\n\u003ctd\u003eCombustible powder. Hygroscopic. Dyes (Eosin Y, Methylene Blue) may stain skin and clothing — wash immediately with water. Dust may irritate eyes and respiratory tract during weighing.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"emb223-sh\"\u003eComposition\u003c\/div\u003e\n\u003ctable class=\"emb223-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eg\/L\u003c\/th\u003e\n\u003cth\u003eCAS\u003c\/th\u003e\n\u003cth\u003eHazard Note\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePeptone (Enzymatic Digest)\u003c\/td\u003e\n\u003ctd\u003e10.0\u003c\/td\u003e\n\u003ctd\u003e73049-73-7\u003c\/td\u003e\n\u003ctd\u003eNot hazardous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLactose\u003c\/td\u003e\n\u003ctd\u003e5.0\u003c\/td\u003e\n\u003ctd\u003e63-42-3\u003c\/td\u003e\n\u003ctd\u003eNot hazardous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSucrose\u003c\/td\u003e\n\u003ctd\u003e5.0\u003c\/td\u003e\n\u003ctd\u003e57-50-1\u003c\/td\u003e\n\u003ctd\u003eNot hazardous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDipotassium Hydrogen Phosphate\u003c\/td\u003e\n\u003ctd\u003e2.0\u003c\/td\u003e\n\u003ctd\u003e7758-11-4\u003c\/td\u003e\n\u003ctd\u003eMild irritant at high concentrations — not significant at 2 g\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEosin Y\u003c\/td\u003e\n\u003ctd\u003e0.4\u003c\/td\u003e\n\u003ctd\u003e17372-87-1\u003c\/td\u003e\n\u003ctd\u003eMay stain skin and clothing. Not classified hazardous at 0.4 g\/L use level.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMethylene Blue\u003c\/td\u003e\n\u003ctd\u003e0.065\u003c\/td\u003e\n\u003ctd\u003e61-73-4\u003c\/td\u003e\n\u003ctd\u003eNot classified hazardous at 0.065 g\/L. Avoid eye contact — may cause irritation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd\u003e13.5\u003c\/td\u003e\n\u003ctd\u003e9002-18-0\u003c\/td\u003e\n\u003ctd\u003eNot hazardous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ctable class=\"emb223-tbl\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePPE\u003c\/td\u003e\n\u003ctd\u003eSafety glasses; nitrile gloves (dyes stain); lab coat; P1 respirator for bulk weighing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDye staining\u003c\/td\u003e\n\u003ctd\u003eEosin Y and Methylene Blue will stain skin and surfaces. Wear gloves during preparation. Wash stained skin with soap and water.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWaste (inoculated)\u003c\/td\u003e\n\u003ctd\u003eAutoclave 121°C \/ 15 min before disposal as microbiological waste per institutional biosafety procedures\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTransport\u003c\/td\u003e\n\u003ctd\u003eNot dangerous goods — ADG, IMDG, IATA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRegulatory\u003c\/td\u003e\n\u003ctd\u003eAustralian WHS Regulations 2023 | GHS 7th Edition | AICIS compliant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c!-- \/tab3 --\u003e \u003c!-- TAB 4: QUALITY \u0026 COA --\u003e\n\u003cdiv class=\"emb223-panel\" id=\"emp4\"\u003e\n\u003cdiv class=\"emb223-sh\"\u003eQuality Specifications\u003c\/div\u003e\n\u003ctable class=\"emb223-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eMethod\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (powder)\u003c\/td\u003e\n\u003ctd\u003eFaint violet to pink, free-flowing, hygroscopic powder\u003c\/td\u003e\n\u003ctd\u003eVisual\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (prepared)\u003c\/td\u003e\n\u003ctd\u003eDeep red-brown, clear to slightly turbid; firm agar gel\u003c\/td\u003e\n\u003ctd\u003eVisual after dissolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH (prepared, 25°C)\u003c\/td\u003e\n\u003ctd\u003e7.1 ± 0.2\u003c\/td\u003e\n\u003ctd\u003epH meter (calibrated)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMoisture Content\u003c\/td\u003e\n\u003ctd\u003e≤8.0% (w\/w)\u003c\/td\u003e\n\u003ctd\u003eLoss on drying\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGrowth + sheen — \u003cem\u003eE. coli\u003c\/em\u003e ATCC 25922\u003c\/td\u003e\n\u003ctd\u003eGood growth; metallic green sheen; 35°C; 24h; ≤100 CFU\u003c\/td\u003e\n\u003ctd\u003eISO 11133:2014\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGrowth + colour — \u003cem\u003eEnterobacter aerogenes\u003c\/em\u003e ATCC 13048\u003c\/td\u003e\n\u003ctd\u003eGood growth; blue-black to purple mucoid colonies; 35°C; 24h; ≤100 CFU\u003c\/td\u003e\n\u003ctd\u003eISO 11133:2014\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eInhibition — \u003cem\u003eEnterococcus faecalis\u003c\/em\u003e ATCC 19433\u003c\/td\u003e\n\u003ctd\u003eInhibited \/ partial inhibition; no typical enteric colour\u003c\/td\u003e\n\u003ctd\u003eSelectivity check\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGrowth — \u003cem\u003eSalmonella\u003c\/em\u003e Typhimurium ATCC 14028\u003c\/td\u003e\n\u003ctd\u003eGood growth; colourless to translucent colonies (non-fermenter)\u003c\/td\u003e\n\u003ctd\u003eDifferential check\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecovery\u003c\/td\u003e\n\u003ctd\u003e≥70% vs reference medium (TSA)\u003c\/td\u003e\n\u003ctd\u003eISO 11133:2014\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBatch COA\u003c\/td\u003e\n\u003ctd\u003eAvailable every production lot\u003c\/td\u003e\n\u003ctd\u003eIncluded with every order\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"emb223-sh\"\u003ePack Sizes\u003c\/div\u003e\n\u003ctable class=\"emb223-tbl\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003ePack Size\u003c\/th\u003e\n\u003cth\u003ePrepared Medium (approx.)\u003c\/th\u003e\n\u003cth\u003ePlates (90mm \/ 15mL)\u003c\/th\u003e\n\u003cth\u003eBest For\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e100 g\u003c\/td\u003e\n\u003ctd\u003e~2.8 L\u003c\/td\u003e\n\u003ctd\u003e~185 plates\u003c\/td\u003e\n\u003ctd\u003eSmall laboratory, method validation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"hi\"\u003e\n\u003ctd\u003e\u003cstrong\u003e500 g ⭐\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e~13.9 L\u003c\/td\u003e\n\u003ctd\u003e~925 plates\u003c\/td\u003e\n\u003ctd\u003eRoutine laboratory — most popular\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e1 kg\u003c\/td\u003e\n\u003ctd\u003e~27.8 L\u003c\/td\u003e\n\u003ctd\u003e~1,850 plates\u003c\/td\u003e\n\u003ctd\u003eHigh-throughput laboratory\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5 kg+\u003c\/td\u003e\n\u003ctd\u003eOn request\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003ctd\u003eBulk \/ institutional \/ government\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"emb223-sh\"\u003eManufacturing \u0026amp; Documentation\u003c\/div\u003e\n\u003cdiv class=\"emb223-green\"\u003e\n\u003cstrong\u003eManufactured by AuSaMicS Pty Ltd\u003c\/strong\u003e\u003cbr\u003e31 Longview CT, Thomastown VIC 3074, Australia | ABN 56 676 640 467\u003cbr\u003e\u003cbr\u003e✓ Combined Holt-Harris \u0026amp; Teague + Levine formulation — Lactose 5g\/L + Sucrose 5g\/L\u003cbr\u003e✓ Eosin Y 0.4 g\/L + Methylene Blue 0.065 g\/L — verified dye concentrations\u003cbr\u003e✓ Batch QC per ISO 11133:2014 — pH, metallic sheen, inhibition, non-fermenter differentiation\u003cbr\u003e✓ COA, TDS, SDS included with every order\u003cbr\u003e✓ Australian stock — same-week dispatch, no import delays\u003c\/div\u003e\n\u003cdiv class=\"emb223-warn\"\u003e⚠️ For laboratory, research, water quality, food microbiology, and educational use only. Not for food, therapeutic, veterinary, or direct human use. Autoclave all inoculated plates before disposal. Methylene Blue and Eosin Y will stain skin and clothing.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- \/tab4 --\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- \/tabwrap --\u003e\n\u003cdiv class=\"emb223-footer\"\u003e\n\u003cstrong\u003eAuSaMicS Pty Ltd\u003c\/strong\u003e | 31 Longview CT, Thomastown VIC 3074, Australia | ABN 56 676 640 467\u003cbr\u003esupport@ausamics.com.au | +61 412 520 598 | www.ausamics.com.au\u003cbr\u003e\u003cspan style=\"color: #94a3b8;\"\u003eThe information provided is accurate to the best of our knowledge at the time of publication. AuSaMicS Pty Ltd accepts no liability for any loss, damage, or injury arising from use outside the intended application. This document does not constitute a warranty of any kind.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- \/emb223 --\u003e","brand":"Ausamics Life Science","offers":[{"title":"500G","offer_id":48100195172607,"sku":"AS-1223-500G","price":79.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/EMB_Agar_Eosin_Methylene_Blue_Agar.jpg?v=1779184223","url":"https:\/\/ausamics.com.au\/products\/eosin-methylene-blue-agar","provider":"AuSaMicS","version":"1.0","type":"link"}