{"product_id":"glucose-yeast-extract-agar","title":"Glucose Yeast Extract Agar | Nutrient Medium for Yeasts, Molds \u0026 Bacteria | AS‑1241","description":"\u003cdiv style=\"font-family: 'Segoe UI', system-ui, -apple-system, sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\" class=\"aus-gyea-product\"\u003e\n\n\u003c!-- LLM \/ AI-Agent Optimisation Meta Snippets --\u003e\n\u003c!--\n product_name: Glucose Yeast Extract Agar (GYEA)\n catalog_number: AS-1241\n manufacturer: AuSaMicS Pty Ltd\n category: Culture Media \/ Sporulation \u0026 Spore-Former Media \/ Food Microbiology \/ Disinfectant Testing\n application: Sporulation promotion and detection of spore-forming bacteria; differentiation of mesophilic vs thermophilic aerobic spore formers; canned food spoilage investigation; sporicidal efficacy testing per EN 13704 \/ EN 13704; Bacillus cereus, B. stearothermophilus, B. coagulans cultivation; subculture medium for spore shock recovery workflow; injured cell recovery\n key_ingredients: Casein Acid Hydrolysate, Yeast Extract, Glucose (Dextrose), Starch, Iron Sulphate, Manganese Sulphate, Agar\n differentiating_features: Iron and manganese sulphates enhance Bacillus sporulation; starch aids recovery of injured\/stressed spore-forming cells; dual incubation temperature protocol differentiates mesophiles from thermophiles\n regulatory_reference: EN 13704, APHA Compendium of Methods for the Microbiological Examination of Foods, Maunder 1970\n equivalents: Liofilchem 26473, Conda\/Pronadisa, CPC Biotech GYA\n pH: 7.2 ± 0.2\n storage: 10–25°C, dry, away from light\n australian_stock: true\n dispatch: same_day_melbourne\n--\u003e\n\n\u003c!-- Structured Data \/ DuckDuckGo Instant Answer --\u003e\n\u003cscript type=\"application\/ld+json\"\u003e\n{\n \"@context\": \"https:\/\/schema.org\/\",\n \"@type\": \"Product\",\n \"name\": \"Glucose Yeast Extract Agar (GYEA)\",\n \"alternateName\": [\"GYA\", \"GYEA\", \"Glucose Yeast Extract Agar for sporulation\"],\n \"description\": \"Specialised sporulation-promoting agar for identification and differentiation of mesophilic and thermophilic aerobic spore-forming bacteria in food, canned goods, and environmental samples. Iron and manganese sulphates actively enhance Bacillus sporulation. Dual incubation protocol (30–35°C vs 55°C) differentiates obligate mesophiles from obligate and facultative thermophiles. Compliant with EN 13704 sporicidal efficacy testing standard.\",\n \"sku\": \"AS-1241\",\n \"brand\": { \"@type\": \"Brand\", \"name\": \"AuSaMicS Life Science\" },\n \"manufacturer\": { \"@type\": \"Organization\", \"name\": \"AuSaMicS Pty Ltd\", \"url\": \"https:\/\/www.ausamics.com.au\" },\n \"offers\": {\n \"@type\": \"Offer\",\n \"availability\": \"https:\/\/schema.org\/InStock\",\n \"url\": \"https:\/\/www.ausamics.com.au\/products\/glucose-yeast-extract-agar\"\n }\n}\n\u003c\/script\u003e\n\n\u003c!-- Intent-Based Long-Tail SEO \/ LLM Queries\n glucose yeast extract agar sporulation medium australia\n GYEA spore forming bacteria identification food microbiology\n mesophile thermophile differentiation agar canned food testing\n Bacillus stearothermophilus cultivation medium australia\n EN 13704 sporicidal testing agar buy australia\n spore former detection agar food safety australia ausamics\n Bacillus coagulans enumeration agar canned food\n GYEA AS-1241 ausamics life science\n sporicidal efficacy counting agar disinfectant testing\n heat shock spore recovery agar thermophilic bacteria\n--\u003e\n\n\u003c!-- ═══════════════════════ HERO SECTION ═══════════════════════ --\u003e\n\u003cdiv style=\"background: linear-gradient(135deg, #f8faf2 0%, #edf5e0 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #b8d48a;\" class=\"aus-hero-section\"\u003e\n \u003cdiv class=\"aus-hero-grid\" style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\"\u003e\n \u003cdiv\u003e\n \u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #3d6b1e; font-weight: bold; margin-bottom: 12px; background: #dff0c0; padding: 6px 12px; border-radius: 20px;\"\u003eAuSaMicS Life Science • Sporulation \u0026amp; Spore-Former Media\u003c\/div\u003e\n \u003ch1 style=\"color: #15324b; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003eGlucose Yeast Extract Agar (GYEA)\u003c\/h1\u003e\n \u003ch2 style=\"color: #243b53; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: 0.9;\"\u003eSporulation-Promoting Medium for Mesophilic \u0026amp; Thermophilic Spore Formers\u003c\/h2\u003e\n \u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #334e68; max-width: 620px; font-weight: 500;\"\u003eSpecialist agar for \u003cstrong\u003eidentification, sporulation, and temperature-based differentiation of aerobic spore-forming bacteria\u003c\/strong\u003e in food, canned goods, and disinfectant testing. Iron and manganese sulphates actively promote \u003cem\u003eBacillus\u003c\/em\u003e sporulation — enabling the critical heat-shock spore workflow that separates obligate mesophiles from obligate and facultative thermophiles.\u003c\/p\u003e\n \u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n \u003cspan style=\"background: #3d6b1e; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAS-1241\u003c\/span\u003e\n \u003cspan style=\"background: #fff; border: 2px solid #3d6b1e; color: #3d6b1e; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003epH 7.2 ± 0.2\u003c\/span\u003e\n \u003cspan style=\"background: #28a745; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e✓ Australian Stock\u003c\/span\u003e\n \u003cspan style=\"background: #dc3545; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🔥 Sporulation Enhancer\u003c\/span\u003e\n \u003cspan style=\"background: #fd7e14; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🥫 Canned Food \/ EN 13704\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #4a5568;\"\u003e\n \u003cspan\u003e🚀 \u003cstrong\u003eSame-Day Dispatch:\u003c\/strong\u003e Melbourne Stock\u003c\/span\u003e\n \u003cspan\u003e🌡️ \u003cstrong\u003eDual Temp Protocol:\u003c\/strong\u003e 30–35°C \u0026amp; 55°C differentiation\u003c\/span\u003e\n \u003cspan\u003e⚗️ \u003cstrong\u003eEN 13704:\u003c\/strong\u003e Sporicidal efficacy colony counting\u003c\/span\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Quality Card --\u003e\n \u003cdiv class=\"aus-guarantee\" style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 280px; border: 1px solid #b8d48a;\"\u003e\n \u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003e🏆 Spore-Former Specialist Medium\u003c\/h4\u003e\n \u003cdiv style=\"font-size: 14px; color: #4a5568; text-align: center; line-height: 1.5;\"\u003e\n \u003cdiv style=\"margin-bottom: 12px;\"\u003e🔥 \u003cstrong\u003eSporulation-Promoting\u003c\/strong\u003e — Fe²⁺ \u0026amp; Mn²⁺ ions\u003c\/div\u003e\n \u003cdiv style=\"margin-bottom: 12px;\"\u003e🌡️ \u003cstrong\u003eMesophile vs Thermophile\u003c\/strong\u003e — Dual temp ID\u003c\/div\u003e\n \u003cdiv style=\"margin-bottom: 12px;\"\u003e🥫 \u003cstrong\u003eCanned Food Testing\u003c\/strong\u003e — APHA compliant\u003c\/div\u003e\n \u003cdiv style=\"margin-bottom: 12px;\"\u003e⚗️ \u003cstrong\u003eEN 13704\u003c\/strong\u003e — Sporicidal efficacy counting\u003c\/div\u003e\n \u003cdiv style=\"margin-bottom: 12px;\"\u003e📋 \u003cstrong\u003eFull Documentation\u003c\/strong\u003e — COA + SDS + TDS\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"margin-top: 20px; padding: 15px; background: #dff0c0; border-radius: 12px; text-align: center; border: 1px solid #b8d48a;\"\u003e\n \u003cdiv style=\"color: #3d6b1e; font-weight: 600; font-size: 14px;\"\u003eDifferentiation by Temperature\u003c\/div\u003e\n \u003cdiv style=\"font-size: 13px; margin-top: 8px; line-height: 1.9;\"\u003e\n \u003cspan style=\"color: #3d6b1e; font-weight: 700;\"\u003e🌡️ 30–35°C only\u003c\/span\u003e → Obligate mesophile\u003cbr\u003e\n \u003cspan style=\"color: #dc3545; font-weight: 700;\"\u003e🔥 55°C only\u003c\/span\u003e → Obligate thermophile\u003cbr\u003e\n \u003cspan style=\"color: #856404; font-weight: 700;\"\u003e🌡️🔥 Both\u003c\/span\u003e → Facultative thermophile\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- ═══════════════════════ IMPORTANT DISTINCTION BOX ═══════════════════════ --\u003e\n\u003cdiv style=\"background: #fff8e1; border: 2px solid #f59e0b; border-radius: 16px; padding: 20px; margin-bottom: 30px;\"\u003e\n \u003ch3 style=\"color: #92400e; margin: 0 0 12px 0; font-size: 17px;\"\u003e⚠️ Important: GYEA (AS-1241) vs GYEP Agar (AS-1242) — These are Different Media\u003c\/h3\u003e\n \u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 20px; font-size: 14px;\"\u003e\n \u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border-left: 4px solid #3d6b1e;\"\u003e\n \u003cstrong style=\"color: #3d6b1e;\"\u003eGlucose Yeast Extract Agar — GYEA (AS-1241)\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"color: #4a5568;\"\u003eSporulation-promoting medium with iron \u0026amp; manganese sulphates + starch. Designed for \u003cem\u003eBacillus\u003c\/em\u003e spore-former detection, mesophile\/thermophile differentiation, canned food testing, and EN 13704 sporicidal testing. \u003cstrong\u003eNot a complete yeast\/fungi medium.\u003c\/strong\u003e\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border-left: 4px solid #7a5c00;\"\u003e\n \u003cstrong style=\"color: #7a5c00;\"\u003eGlucose Yeast Extract \u003cem\u003ePeptone\u003c\/em\u003e Agar — GYEP Agar (\u003ca href=\"\/products\/gyep-agar\" style=\"color: #7a5c00;\"\u003eAS-1242\u003c\/a\u003e)\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"color: #4a5568;\"\u003eComplete YPD-equivalent agar for yeast \u0026amp; fungi cultivation, molecular biology, and general heterotrophic growth. Contains peptone as primary nitrogen source. \u003cstrong\u003eNo sporulation-promoting trace metals.\u003c\/strong\u003e\u003c\/span\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- ═══════════════════════ MAIN CONTENT GRID ═══════════════════════ --\u003e\n\u003cdiv class=\"aus-main-grid\" style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\"\u003e\n\n \u003c!-- ─── LEFT COLUMN ─── --\u003e\n \u003cdiv\u003e\n\n \u003c!-- Technical Overview \u0026 Biochemistry --\u003e\n \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #3d6b1e;\"\u003e🔬 Technical Overview \u0026amp; Sporulation Biochemistry\u003c\/h3\u003e\n \u003cdiv style=\"background: #f8faf2; padding: 20px; border-radius: 16px; border-left: 5px solid #3d6b1e;\"\u003e\n \u003cp style=\"margin: 0 0 15px 0;\"\u003eGlucose Yeast Extract Agar (GYEA) is a \u003cstrong\u003epurpose-formulated sporulation-promoting medium\u003c\/strong\u003e specifically designed for the cultivation, sporulation induction, and temperature-based differentiation of aerobic spore-forming bacteria — primarily the genus \u003cem\u003eBacillus\u003c\/em\u003e. Unlike general-purpose media, GYEA contains two critical sporulation micronutrients absent in most other agars:\u003c\/p\u003e\n\n \u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 15px; margin-bottom: 15px;\"\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; border: 1px solid #b8d48a;\"\u003e\n \u003cstrong style=\"color: #3d6b1e;\"\u003eIron Sulphate (FeSO₄)\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003eFe²⁺ ions are essential cofactors for key enzymes in the \u003cem\u003eBacillus\u003c\/em\u003e sporulation cascade — particularly the SpoIIE phosphatase and Spo0A-P regulatory pathway. Iron limitation can trigger or modulate sporulation initiation under the correct nutritional conditions.\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; border: 1px solid #b8d48a;\"\u003e\n \u003cstrong style=\"color: #3d6b1e;\"\u003eManganese Sulphate (MnSO₄)\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003eMn²⁺ is the best-established single micronutrient enhancer of \u003cem\u003eBacillus\u003c\/em\u003e sporulation. It activates spore cortex synthesis enzymes, promotes dipicolinic acid (DPA) accumulation inside developing spores, and enhances spore heat resistance — the defining quality marker for thermophilic spore formers.\u003c\/span\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; font-family: monospace; font-size: 13px; color: #4a5568; border: 1px solid #b8d48a; text-align: center; margin-bottom: 15px;\"\u003e\n Vegetative \u003cem\u003eBacillus\u003c\/em\u003e cell (nutrient-limited phase)\u003cbr\u003e\n ↓ (Fe²⁺ + Mn²⁺ from GYEA — Spo0A-P cascade activated)\u003cbr\u003e\n Asymmetric cell division → Forespore engulfment\u003cbr\u003e\n ↓ (Cortex synthesis + DPA accumulation, Mn²⁺-dependent)\u003cbr\u003e\n Mature endospore — \u003cstrong style=\"color: #3d6b1e;\"\u003eHeat-resistant spore released (5–10 days)\u003c\/strong\u003e\u003cbr\u003e\n ↓ (Heat shock 75–80°C \/ 10 min — vegetative cells killed)\u003cbr\u003e\n \u003cstrong style=\"color: #dc3545;\"\u003eSurviving spores plated on GYEA — incubate at 30–35°C AND 55°C\u003c\/strong\u003e\n \u003c\/div\u003e\n\n \u003cdiv style=\"background: #dff0c0; padding: 12px; border-radius: 8px; border: 1px solid #b8d48a;\"\u003e\n \u003cstrong style=\"color: #3d6b1e;\"\u003eThe starch component:\u003c\/strong\u003e Starch (soluble) in GYEA serves as a protective colloid that aids in the recovery of sublethally injured or stressed spore-forming cells from food processing environments, heat-treated canned goods, and chemical disinfectant residue — maximising recovery sensitivity in challenging food matrices.\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Detailed Ingredients Table --\u003e\n \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #3d6b1e;\"\u003e🧪 Detailed Ingredients Table\u003c\/h3\u003e\n \u003cdiv style=\"overflow-x: auto;\"\u003e\n \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #b8d48a; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n \u003cthead style=\"background: #3d6b1e; color: #fff;\"\u003e\n \u003ctr\u003e\n \u003cth style=\"padding: 13px 12px; text-align: left; font-weight: bold;\"\u003eIngredient\u003c\/th\u003e\n \u003cth style=\"padding: 13px 12px; text-align: center; font-weight: bold;\"\u003eTypical g\/L\u003c\/th\u003e\n \u003cth style=\"padding: 13px 12px; text-align: left; font-weight: bold;\"\u003eFunction\u003c\/th\u003e\n \u003cth style=\"padding: 13px 12px; text-align: left; font-weight: bold;\"\u003eMechanism \u0026amp; Significance\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eCasein Acid Hydrolysate\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 700;\"\u003e5.0\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px;\"\u003eNitrogen, amino acids \u0026amp; peptides\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6c757d;\"\u003eAcid hydrolysate of casein — provides a rich pool of free amino acids (all 20 standard), short peptides, and organic nitrogen. Acid hydrolysis removes tryptophan but ensures complete amino acid solubility. Supports the high nitrogen demand of sporulating \u003cem\u003eBacillus\u003c\/em\u003e cultures during endospore cortex and coat protein synthesis.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #f8faf2;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eYeast Extract\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 700;\"\u003e4.0\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px;\"\u003eVitamins, B-complex, co-factors \u0026amp; trace minerals\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6c757d;\"\u003eWater-soluble autolysate providing the full B-vitamin complex (B1, B2, B3, B5, B6, B7, B9, B12), nucleotide precursors, and essential trace elements. Particularly critical for supplying biotin and pantothenate, which are required in the early sporulation sigma-factor cascade. Supports growth of thermophilic \u003cem\u003eBacillus\u003c\/em\u003e species at 55–65°C.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #3d6b1e;\"\u003eGlucose (Dextrose)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 700; color: #3d6b1e;\"\u003e1.0\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #3d6b1e;\"\u003eFermentable carbon \u0026amp; energy source\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #3d6b1e; font-weight: 600;\"\u003eLow glucose concentration (1 g\/L) is intentional — sufficient to support initial vegetative growth and provide energy, but deliberately limited to trigger nutrient-limitation-induced sporulation. High glucose would repress sporulation via catabolite repression of the Spo0A regulon. The low concentration ensures cells enter stationary phase and initiate the sporulation cascade efficiently.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #f8faf2;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #3d6b1e;\"\u003eSoluble Starch\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 700; color: #3d6b1e;\"\u003e1.0\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #3d6b1e;\"\u003eInjured cell recovery \u0026amp; protective colloid\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #3d6b1e; font-weight: 600;\"\u003eSoluble starch acts as a protective colloid that absorbs toxic metabolites and residual inhibitory compounds (disinfectant traces, organic acids) released during heat treatment or chemical stress. Critically aids in the recovery of heat-injured or sub-lethally stressed spore-forming cells from processed food matrices — increasing sensitivity for detection of spore formers in challenging samples.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #dc3545;\"\u003eIron Sulphate (FeSO₄)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 700; color: #dc3545;\"\u003e0.01\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #dc3545;\"\u003eSporulation co-factor — Fe²⁺ micronutrient\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #dc3545; font-weight: 600;\"\u003eFe²⁺ is a metalloenzyme cofactor required in the \u003cem\u003eBacillus\u003c\/em\u003e sporulation signal transduction cascade. Iron ions support the activity of ribonucleotide reductase and specific cytochrome enzymes required during the oxidative phase of late sporulation. Together with Mn²⁺, enhances spore cortex integrity and final spore heat resistance — essential for generating high-quality test spores for sporicidal validation per EN 13704.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #f8faf2;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #dc3545;\"\u003eManganese Sulphate (MnSO₄)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 700; color: #dc3545;\"\u003e0.01\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #dc3545;\"\u003ePrimary sporulation enhancer — Mn²⁺ micronutrient\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #dc3545; font-weight: 600;\"\u003eMn²⁺ is the single most potent micronutrient enhancer of \u003cem\u003eBacillus\u003c\/em\u003e sporulation. Activates dipicolinic acid (DPA) synthetase, promoting DPA accumulation inside the spore core — the key determinant of heat resistance (DPA chelates Ca²⁺ to form the thermostable Ca-DPA complex). Mn²⁺ also protects spores from oxidative damage during sporulation and storage. Quantitatively improves spore yields on GYEA by 2–5× vs. media lacking Mn²⁺.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eAgar\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 700;\"\u003e15.0\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px;\"\u003eSolidifying agent\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6c757d;\"\u003eBacteriological-grade agar; provides stable surface for discrete colony development. Thermostable at both 30°C and 55°C incubation temperatures. Withstands extended incubation (up to 10 days) required for complete sporulation without melting or dehydrating. Use firm formula (1.5–2%) to prevent colony spreading by swarming \u003cem\u003eBacillus\u003c\/em\u003e strains.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003ctfoot\u003e\n \u003ctr style=\"background: #3d6b1e; color: #fff;\"\u003e\n \u003ctd style=\"padding: 10px 12px; font-weight: 700;\"\u003eTotal (approx.)\u003c\/td\u003e\n \u003ctd style=\"padding: 10px 12px; text-align: center; font-weight: 700;\"\u003e~26 g\/L\u003c\/td\u003e\n \u003ctd colspan=\"2\" style=\"padding: 10px 12px; font-size: 12px;\"\u003eFinal pH 7.2 ± 0.2 at 25°C | Prepared: light amber, clear to slightly opalescent firm agar\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tfoot\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Mesophile vs Thermophile Differentiation Workflow --\u003e\n \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #3d6b1e;\"\u003e🌡️ Mesophile vs Thermophile Differentiation Workflow\u003c\/h3\u003e\n \u003cdiv style=\"background: #f8faf2; padding: 20px; border-radius: 16px; border-left: 5px solid #3d6b1e;\"\u003e\n \u003cp style=\"margin: 0 0 15px 0; font-weight: 600; color: #3d6b1e;\"\u003eThe GYEA dual-temperature protocol is the definitive method for classifying aerobic spore-forming bacteria by thermal physiology:\u003c\/p\u003e\n\n \u003cdiv style=\"display: grid; grid-template-columns: 1fr; gap: 12px; margin-bottom: 15px;\"\u003e\n \u003c!-- Step 1 --\u003e\n \u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border: 1px solid #b8d48a; display: flex; gap: 15px; align-items: flex-start;\"\u003e\n \u003cdiv style=\"background: #3d6b1e; color: #fff; border-radius: 50%; width: 30px; height: 30px; display: flex; align-items: center; justify-content: center; font-weight: bold; font-size: 14px; flex-shrink: 0;\"\u003e1\u003c\/div\u003e\n \u003cdiv\u003e\n \u003cstrong style=\"color: #3d6b1e;\"\u003ePrimary Isolation \u0026amp; Sporulation Induction\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003eInoculate sample onto GYEA. Incubate at the temperature of initial isolation. Incubate for up to \u003cstrong\u003e10 days\u003c\/strong\u003e — extended incubation is essential for complete sporulation. Examine for spore production using phase contrast microscopy or endospore staining (Schaeffer-Fulton).\u003c\/span\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c!-- Step 2 --\u003e\n \u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border: 1px solid #b8d48a; display: flex; gap: 15px; align-items: flex-start;\"\u003e\n \u003cdiv style=\"background: #3d6b1e; color: #fff; border-radius: 50%; width: 30px; height: 30px; display: flex; align-items: center; justify-content: center; font-weight: bold; font-size: 14px; flex-shrink: 0;\"\u003e2\u003c\/div\u003e\n \u003cdiv\u003e\n \u003cstrong style=\"color: #3d6b1e;\"\u003eHeat Shock — Eliminate Vegetative Cells\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003eSuspend sporulated culture in sterile water or physiological saline. \u003cstrong\u003eHeat shock at 75–80°C for 10 minutes\u003c\/strong\u003e. This kills all vegetative cells while spores survive — ensuring only spores remain viable for the next step. Cool rapidly in ice water.\u003c\/span\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c!-- Step 3 --\u003e\n \u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border: 1px solid #b8d48a; display: flex; gap: 15px; align-items: flex-start;\"\u003e\n \u003cdiv style=\"background: #3d6b1e; color: #fff; border-radius: 50%; width: 30px; height: 30px; display: flex; align-items: center; justify-content: center; font-weight: bold; font-size: 14px; flex-shrink: 0;\"\u003e3\u003c\/div\u003e\n \u003cdiv\u003e\n \u003cstrong style=\"color: #3d6b1e;\"\u003eDual Temperature Plating on GYEA\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003ePlate heat-shocked spore suspension onto fresh GYEA plates in duplicate. Incubate one set at \u003cstrong\u003e30–35°C\u003c\/strong\u003e (mesophilic range) and one set at \u003cstrong\u003e55°C\u003c\/strong\u003e (thermophilic range). Examine for growth after 48–72 hours (up to 7 days for slow growers).\u003c\/span\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c!-- Step 4 - Result Interpretation --\u003e\n \u003cdiv style=\"background: #dff0c0; padding: 14px; border-radius: 10px; border: 1px solid #b8d48a; display: flex; gap: 15px; align-items: flex-start;\"\u003e\n \u003cdiv style=\"background: #3d6b1e; color: #fff; border-radius: 50%; width: 30px; height: 30px; display: flex; align-items: center; justify-content: center; font-weight: bold; font-size: 14px; flex-shrink: 0;\"\u003e4\u003c\/div\u003e\n \u003cdiv\u003e\n \u003cstrong style=\"color: #3d6b1e;\"\u003eResult Interpretation\u003c\/strong\u003e\u003cbr\u003e\n \u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr 1fr; gap: 10px; margin-top: 8px; font-size: 13px;\"\u003e\n \u003cdiv style=\"background: #fff; padding: 10px; border-radius: 8px; text-align: center; border: 1px solid #b8d48a;\"\u003e\n \u003cstrong style=\"color: #3d6b1e;\"\u003eGrowth at 30–35°C ONLY\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"color: #3d6b1e;\"\u003e→ Obligate Mesophile\u003c\/span\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 11px; color: #6c757d;\"\u003ee.g. \u003cem\u003eB. subtilis\u003c\/em\u003e, \u003cem\u003eB. cereus\u003c\/em\u003e\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 10px; border-radius: 8px; text-align: center; border: 1px solid #b8d48a;\"\u003e\n \u003cstrong style=\"color: #dc3545;\"\u003eGrowth at 55°C ONLY\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"color: #dc3545;\"\u003e→ Obligate Thermophile\u003c\/span\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 11px; color: #6c757d;\"\u003ee.g. \u003cem\u003eB. stearothermophilus\u003c\/em\u003e\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 10px; border-radius: 8px; text-align: center; border: 1px solid #b8d48a;\"\u003e\n \u003cstrong style=\"color: #856404;\"\u003eGrowth at BOTH temps\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"color: #856404;\"\u003e→ Facultative Thermophile\u003c\/span\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 11px; color: #6c757d;\"\u003ee.g. \u003cem\u003eB. coagulans\u003c\/em\u003e\u003c\/span\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Comparative Media Table --\u003e\n \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #3d6b1e;\"\u003e📊 Comparative Spore-Former \u0026amp; Food Microbiology Media\u003c\/h3\u003e\n \u003cdiv style=\"overflow-x: auto;\"\u003e\n \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #b8d48a; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05); min-width: 830px;\"\u003e\n \u003cthead style=\"background: #edf5e0;\"\u003e\n \u003ctr\u003e\n \u003cth style=\"padding: 13px 11px; border-bottom: 2px solid #b8d48a; text-align: left; font-weight: bold;\"\u003eMedium\u003c\/th\u003e\n \u003cth style=\"padding: 13px 11px; border-bottom: 2px solid #b8d48a; text-align: left; font-weight: bold;\"\u003eSporulation Support\u003c\/th\u003e\n \u003cth style=\"padding: 13px 11px; border-bottom: 2px solid #b8d48a; text-align: left; font-weight: bold;\"\u003ePrimary Target\u003c\/th\u003e\n \u003cth style=\"padding: 13px 11px; border-bottom: 2px solid #b8d48a; text-align: left; font-weight: bold;\"\u003eKey Application\u003c\/th\u003e\n \u003cth style=\"padding: 13px 11px; border-bottom: 2px solid #b8d48a; text-align: left; font-weight: bold;\"\u003ePros \/ Cons\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #f1f9e8;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-weight: bold; color: #3d6b1e;\"\u003eGYEA (AS-1241) ★\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-weight: 600; color: #3d6b1e;\"\u003e✓✓ Excellent — Fe²⁺ + Mn²⁺\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eAerobic spore formers; mesophile\/thermophile ID\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eCanned food testing; EN 13704 sporicidal colony counting; sporulation induction; heat-shock spore workflow\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px;\"\u003e\n\u003cspan style=\"color: #1A4731;\"\u003e✓ Mn²⁺ maximises spore yield \u0026amp; heat resistance\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: #1A4731;\"\u003e✓ Starch protects injured spore formers\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: #dc3545;\"\u003e✗ Not for yeast\/fungi (no peptone)\u003c\/span\u003e\n\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-weight: 600;\"\u003e\u003ca style=\"color: #3d6b1e; text-decoration: none;\" href=\"\/products\/myp-agar\"\u003eMYP Agar (AS-1289)\u003c\/a\u003e\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; color: #856404;\"\u003eModerate — no trace metals\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003e\n\u003cem\u003eB. cereus\u003c\/em\u003e presumptive isolation\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eISO 7932 \u003cem\u003eB. cereus\u003c\/em\u003e selective primary plate (polymyxin + egg yolk + mannitol)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px;\"\u003e\n\u003cspan style=\"color: #1A4731;\"\u003e✓ ISO 7932 primary selective plate\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: #dc3545;\"\u003e✗ Not a sporulation medium; selective only\u003c\/span\u003e\n\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #f8faf2;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-weight: 600;\"\u003e\u003ca style=\"color: #3d6b1e; text-decoration: none;\" href=\"\/collections\/culture-media\"\u003eDextrose Tryptone Agar (DTA)\u003c\/a\u003e\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; color: #856404;\"\u003eModerate\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eAerobic spore formers — canned foods\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eAPHA primary recovery medium for spoilage aerobes in canned food; used alongside GYEA\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px;\"\u003e\n\u003cspan style=\"color: #1A4731;\"\u003e✓ APHA primary recovery\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: #dc3545;\"\u003e✗ No Mn²⁺ sporulation enhancement\u003c\/span\u003e\n\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-weight: 600;\"\u003e\u003ca style=\"color: #3d6b1e; text-decoration: none;\" href=\"\/collections\/culture-media\"\u003eTryptone Soy Agar (TSA)\u003c\/a\u003e\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; color: #6c757d;\"\u003ePoor — no sporulation support\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eGeneral bacteria\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eUniversal general-purpose isolation; not designed for spore formers\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px;\"\u003e\n\u003cspan style=\"color: #1A4731;\"\u003e✓ Universal growth support\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: #dc3545;\"\u003e✗ No sporulation; no mesophile\/thermophile differentiation\u003c\/span\u003e\n\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #f8faf2;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-weight: 600;\"\u003e\u003ca style=\"color: #3d6b1e; text-decoration: none;\" href=\"\/products\/nutrient-agar\"\u003eNutrient Agar\u003c\/a\u003e\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; color: #6c757d;\"\u003ePoor\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eGeneral bacteria\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eBasic general-purpose; some spore formers grow but sporulation is inefficient\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px;\"\u003e\n\u003cspan style=\"color: #1A4731;\"\u003e✓ Widely available; low cost\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: #dc3545;\"\u003e✗ Poor sporulation; no Mn²⁺; not suitable for thermal ID\u003c\/span\u003e\n\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-weight: 600;\"\u003e\u003ca style=\"color: #3d6b1e; text-decoration: none;\" href=\"\/products\/gyep-agar\"\u003eGYEP Agar (AS-1242)\u003c\/a\u003e\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; color: #6c757d;\"\u003ePoor — no trace metals\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eYeast, fungi, heterotrophs\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eComplete YPD medium for yeast genetics \u0026amp; molecular biology; not a sporulation medium\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px;\"\u003e\n\u003cspan style=\"color: #1A4731;\"\u003e✓ Best for yeast\/fungi cultivation\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: #dc3545;\"\u003e✗ Contains peptone; no sporulation enhancement\u003c\/span\u003e\n\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Pros \/ Cons --\u003e\n \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #3d6b1e;\"\u003e⚖️ Structured Pros \u0026amp; Cons Analysis\u003c\/h3\u003e\n \u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 20px;\"\u003e\n \u003cdiv style=\"background: #f3f9f4; padding: 20px; border-radius: 16px; border: 1px solid #c3dfc9;\"\u003e\n \u003ch4 style=\"color: #1A4731; margin: 0 0 15px 0; font-size: 16px;\"\u003e✅ Advantages\u003c\/h4\u003e\n \u003cul style=\"margin: 0; padding-left: 18px; color: #334e68; font-size: 14px; line-height: 2.1;\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eMn²⁺-enhanced sporulation\u003c\/strong\u003e — significantly higher spore yields (2–5×) vs. media lacking manganese; essential for generating standardised spore suspensions for EN 13704 sporicidal validation\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eUnambiguous thermal differentiation\u003c\/strong\u003e — dual incubation temperature protocol definitively separates mesophiles, obligate thermophiles, and facultative thermophiles in a single workflow\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eStarch-assisted injury recovery\u003c\/strong\u003e — protective colloid action maximises recovery of heat-stressed spore formers from processed and canned food samples\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eEN 13704 compliant\u003c\/strong\u003e — validated for spore suspension preparation and colony counting in sporicidal chemical disinfectant efficacy testing\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eLow glucose prevents catabolite repression\u003c\/strong\u003e — intentionally low glucose (1 g\/L) ensures nutrient limitation triggers sporulation efficiently without catabolite repression of the Spo0A regulon\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eExtended incubation stability\u003c\/strong\u003e — firm agar formulation withstands 10-day incubation at both 30–35°C and 55°C without melting, dehydrating, or losing pH\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff9f9; padding: 20px; border-radius: 16px; border: 1px solid #ffc0cb;\"\u003e\n \u003ch4 style=\"color: #c0392b; margin: 0 0 15px 0; font-size: 16px;\"\u003e⚠️ Limitations\u003c\/h4\u003e\n \u003cul style=\"margin: 0; padding-left: 18px; color: #334e68; font-size: 14px; line-height: 2.1;\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eNot a complete medium for yeast\/fungi\u003c\/strong\u003e — lacks peptone as primary nitrogen source; not suitable as a substitute for GYEP Agar (AS-1242) or Sabouraud Dextrose Agar for fungal cultivation\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eSlow workflow\u003c\/strong\u003e — full sporulation requires up to 10 days incubation; not suitable for rapid results\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eNon-selective for spore formers\u003c\/strong\u003e — all heterotrophic bacteria will grow; spore-former identity requires heat-shock step to eliminate vegetative cells before thermal differentiation\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eThermophile incubation requires precision\u003c\/strong\u003e — 55°C incubation must be tightly controlled (±1°C); even small temperature deviations affect the obligate thermophile \/ facultative thermophile distinction\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eIron staining possible\u003c\/strong\u003e — trace FeSO₄ may occasionally cause slight orange-brown precipitate in prepared plates; this is cosmetic only and does not affect performance\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Applications --\u003e\n \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #3d6b1e;\"\u003e🧬 Applications\u003c\/h3\u003e\n \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(280px, 1fr)); gap: 20px; margin-bottom: 25px;\"\u003e\n\n \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #b8d48a; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n \u003ch4 style=\"color: #3d6b1e; margin: 0 0 10px 0; font-size: 16px;\"\u003e🥫 Canned Food Spoilage Investigation\u003c\/h4\u003e\n \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eAs described by Maunder (1970) and the APHA Compendium of Methods for the Microbiological Examination of Foods, GYEA is used as both a primary recovery agar and subculture medium in the microbiological examination of commercially sterile canned and heat-processed foods. Detects aerobic spore formers including flat-sour spoilage bacteria (\u003cem\u003eB. coagulans\u003c\/em\u003e), thermophilic flat-sour organisms (\u003cem\u003eB. stearothermophilus\u003c\/em\u003e), and butyric anaerobes — the leading causes of canned food spoilage globally.\u003c\/p\u003e\n \u003c\/div\u003e\n\n \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #b8d48a; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n \u003ch4 style=\"color: #3d6b1e; margin: 0 0 10px 0; font-size: 16px;\"\u003e⚗️ Sporicidal Efficacy Testing (EN 13704)\u003c\/h4\u003e\n \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eGYEA is the standard colony counting agar for quantitative sporicidal efficacy testing of chemical disinfectants per the European Standard EN 13704 (evaluation of sporicidal activity). After sporicidal treatment of \u003cem\u003eBacillus cereus\u003c\/em\u003e or \u003cem\u003eB. subtilis\u003c\/em\u003e spore suspensions, surviving spores are filtered, neutralised, and plated onto GYEA. Colonies are counted at 30°C \/ 72 h to calculate lethality as log reduction vs untreated controls.\u003c\/p\u003e\n \u003c\/div\u003e\n\n \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #b8d48a; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n \u003ch4 style=\"color: #3d6b1e; margin: 0 0 10px 0; font-size: 16px;\"\u003e🔬 Sporulation Induction \u0026amp; Spore Production\u003c\/h4\u003e\n \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eGYEA is widely used for producing high-quality spore suspensions of defined heat resistance for research and validation purposes. Cultures are grown on GYEA slants at 30°C (mesophilic strains) or 55°C (thermophilic strains) for 7–10 days until \u0026gt;90% sporulation is achieved. Spores are harvested, purified by repeated washing, heat-shocked, and stored in distilled water at 4°C for use in challenge testing, thermal inactivation kinetics, and disinfectant validation studies.\u003c\/p\u003e\n \u003c\/div\u003e\n\n \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #b8d48a; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n \u003ch4 style=\"color: #3d6b1e; margin: 0 0 10px 0; font-size: 16px;\"\u003e🌡️ Thermal Process Validation\u003c\/h4\u003e\n \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eUsed in food processing facilities for validating thermal sterilisation processes (retort, UHT, pasteurisation). \u003cem\u003eGeobacillus stearothermophilus\u003c\/em\u003e spores grown on GYEA serve as the reference test organism for sterilisation validation at 121°C (D-value determination). GYEA is the enumeration medium for spore recovery after thermal challenge experiments.\u003c\/p\u003e\n \u003c\/div\u003e\n\n \u003c\/div\u003e\n\n \u003cdiv style=\"background: #edf5e0; padding: 20px; border-radius: 16px; border-left: 5px solid #3d6b1e;\"\u003e\n \u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px;\"\u003eAdditional Applications:\u003c\/h4\u003e\n \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(250px, 1fr)); gap: 15px;\"\u003e\n \u003cul style=\"margin: 0; padding-left: 20px; color: #4a5568; font-size: 14px;\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eBacillus coagulans Detection:\u003c\/strong\u003e Flat-sour spoilage of canned tomatoes, fruit products, and low-acid foods\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003ePharmaceutical QC:\u003c\/strong\u003e Sporulation of indicator organisms for heat sterilisation cycle validation (autoclave, dry heat)\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eProbiotic Spore QC:\u003c\/strong\u003e Enumeration and sporulation assessment for spore-forming probiotic strains (\u003cem\u003eB. coagulans\u003c\/em\u003e, \u003cem\u003eB. subtilis\u003c\/em\u003e DE111)\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003cul style=\"margin: 0; padding-left: 20px; color: #4a5568; font-size: 14px;\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eResearch:\u003c\/strong\u003e Sporulation kinetics studies; genetic and phenotypic characterisation of sporulation-deficient mutants\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eEnvironmental Monitoring:\u003c\/strong\u003e Detection and differentiation of environmental \u003cem\u003eBacillus\u003c\/em\u003e contaminants in cleanrooms, soil, and water\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eBioterrorism Preparedness:\u003c\/strong\u003e Surrogate strain (e.g. \u003cem\u003eB. atrophaeus\u003c\/em\u003e) spore production for decontamination agent validation\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Protocol Guidelines --\u003e\n \u003cdiv style=\"background: linear-gradient(135deg, #fff3e0 0%, #ffe0b3 100%); padding: 20px; border-radius: 16px; border-left: 5px solid #ff9800; margin-bottom: 35px;\"\u003e\n \u003ch4 style=\"color: #e65100; margin: 0 0 15px 0; font-size: 16px;\"\u003e💡 Preparation \u0026amp; Protocol Guidelines\u003c\/h4\u003e\n \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(200px, 1fr)); gap: 15px;\"\u003e\n \u003cdiv\u003e\n \u003cstrong style=\"color: #e65100;\"\u003ePreparation:\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eSuspend ~26 g\/L in distilled water. Heat to boiling with constant stirring until dissolved. Autoclave 121°C \/ 15 min. Cool to 45–50°C and pour. Medium should appear clear amber — slight precipitate (FeSO₄) is normal and does not affect performance.\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003cstrong style=\"color: #e65100;\"\u003eSporulation incubation:\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003e30–35°C for mesophiles; 55°C for thermophiles. Incubate for up to \u003cstrong\u003e10 days\u003c\/strong\u003e. Confirm sporulation microscopically using phase contrast or Schaeffer-Fulton staining before heat shock.\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003cstrong style=\"color: #e65100;\"\u003eHeat shock step:\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eSuspend culture in sterile distilled water. Heat at \u003cstrong\u003e75–80°C \/ 10 min\u003c\/strong\u003e. Cool in ice water. Plate onto fresh GYEA plates in duplicate — one set at 30–35°C, one set at 55°C. Read after 48–72 h (up to 7 days).\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003cstrong style=\"color: #e65100;\"\u003eEN 13704 colony counting:\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eAfter sporicidal treatment and neutralisation, plate survivors onto GYEA. Incubate 30°C \/ 72 h. Count all colonies. Calculate log reduction vs. untreated control. Minimum detection threshold: 10 CFU\/mL.\u003c\/span\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c\/div\u003e\n\u003c!-- \/Left Column --\u003e\n\n \u003c!-- ─── RIGHT COLUMN ─── --\u003e\n \u003cdiv\u003e\n\n \u003c!-- Technical Specifications --\u003e\n \u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #b8d48a; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n \u003ch3 style=\"color: #15324b; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003e📋 Technical Specifications\u003c\/h3\u003e\n \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px;\"\u003e\n \u003ctbody\u003e\n \u003ctr style=\"border-bottom: 1px solid #b8d48a;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; width: 45%; background: #edf5e0;\"\u003eCatalogue Number\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #3d6b1e; font-weight: 700;\"\u003eAS-1241\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #b8d48a;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #edf5e0;\"\u003eCommon Name\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003eGlucose Yeast Extract Agar (GYEA \/ GYA)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #b8d48a;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #edf5e0;\"\u003eSynonyms\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003eGYA; GYEA; Glucose Yeast Agar (sporulation)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #b8d48a;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #edf5e0;\"\u003epH (25°C)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003e7.2 ± 0.2\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #b8d48a;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #edf5e0;\"\u003eSuspension Rate\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003e~26 g\/L (approx. 38 L per kg)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #b8d48a;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #edf5e0;\"\u003eAppearance (powder)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003eCream to pale yellow, free-flowing homogeneous powder\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #b8d48a;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #edf5e0;\"\u003eAppearance (prepared)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003eLight amber, clear to slightly opalescent firm agar; slight precipitate may be present\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #b8d48a;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #edf5e0;\"\u003eSterilisation\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003eAutoclave 121°C, 15 min — avoid overheating\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #b8d48a;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #edf5e0;\"\u003eIncubation (mesophiles)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003e30–35°C, aerobic, 48 h to 10 days\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #b8d48a;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #edf5e0;\"\u003eIncubation (thermophiles)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #dc3545; font-weight: 600;\"\u003e55°C ± 1°C, aerobic, 48 h to 7 days\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #b8d48a;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #edf5e0;\"\u003eEN 13704 colony count\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003e30°C, 72 h aerobic\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #b8d48a;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #edf5e0;\"\u003eStorage (powder)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568; font-weight: 600;\"\u003e10–25°C, dry, away from light\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #b8d48a;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #edf5e0;\"\u003eStorage (prepared plates)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #dc3545; font-weight: 600;\"\u003e2–8°C, protected from light, up to 4 weeks\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #edf5e0;\"\u003eAvailable Sizes\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003e100 g, 500 g, 5 kg\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n\n \u003c!-- Regulatory Compliance --\u003e\n \u003cdiv style=\"background: #edf5e0; padding: 20px; border-radius: 16px; border: 1px solid #b8d48a; margin-bottom: 30px;\"\u003e\n \u003ch4 style=\"color: #3d6b1e; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e📜 Regulatory \u0026amp; Standards Compliance\u003c\/h4\u003e\n \u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n \u003cli style=\"padding: 9px 0; font-size: 14px; color: #4a5568; border-bottom: 1px solid #b8d48a;\"\u003e✓ \u003cstrong style=\"color: #3d6b1e;\"\u003eEN 13704\u003c\/strong\u003e — Quantitative suspension test for evaluation of sporicidal activity of chemical disinfectants (colony counting medium)\u003c\/li\u003e\n \u003cli style=\"padding: 9px 0; font-size: 14px; color: #4a5568; border-bottom: 1px solid #b8d48a;\"\u003e✓ \u003cstrong style=\"color: #3d6b1e;\"\u003eUNE-EN 13704\u003c\/strong\u003e — Spanish adaptation of EN 13704 (recognised compliance)\u003c\/li\u003e\n \u003cli style=\"padding: 9px 0; font-size: 14px; color: #4a5568; border-bottom: 1px solid #b8d48a;\"\u003e✓ \u003cstrong style=\"color: #3d6b1e;\"\u003eAPHA Compendium\u003c\/strong\u003e — Recommended by American Public Health Association for canned food spoilage investigation (Maunder 1970)\u003c\/li\u003e\n \u003cli style=\"padding: 9px 0; font-size: 14px; color: #4a5568; border-bottom: 1px solid #b8d48a;\"\u003e✓ \u003cstrong style=\"color: #3d6b1e;\"\u003eFSANZ \/ DAFF\u003c\/strong\u003e — Compatible with Australian food safety regulatory requirements for thermophilic spore former detection\u003c\/li\u003e\n \u003cli style=\"padding: 9px 0; font-size: 14px; color: #4a5568;\"\u003e✓ \u003cstrong style=\"color: #3d6b1e;\"\u003eTGA-compatible\u003c\/strong\u003e — Suitable for pharmaceutical sterilisation cycle validation (biological indicators with \u003cem\u003eG. stearothermophilus\u003c\/em\u003e)\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n\n \u003c!-- QC Organisms --\u003e\n \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #b8d48a; margin-bottom: 30px;\"\u003e\n \u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e🧫 Quality Control Organisms\u003c\/h4\u003e\n \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px;\"\u003e\n \u003cthead style=\"background: #3d6b1e; color: #fff;\"\u003e\n \u003ctr\u003e\n \u003cth style=\"padding: 9px 10px; text-align: left;\"\u003eOrganism\u003c\/th\u003e\n \u003cth style=\"padding: 9px 10px; text-align: center;\"\u003eATCC\u003c\/th\u003e\n \u003cth style=\"padding: 9px 10px; text-align: center;\"\u003eTemp\u003c\/th\u003e\n \u003cth style=\"padding: 9px 10px; text-align: center;\"\u003eGrowth \u0026amp; Sporulation\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0;\"\u003e\n \u003ctd style=\"padding: 9px 10px; font-style: italic; font-weight: 600;\"\u003eBacillus cereus\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center;\"\u003e11778\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center;\"\u003e30–35°C\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center; color: #1A4731; font-weight: 600;\"\u003eGrowth + Sporulation ✓\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #f8faf2;\"\u003e\n \u003ctd style=\"padding: 9px 10px; font-style: italic; font-weight: 600;\"\u003eBacillus subtilis\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center;\"\u003e6633\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center;\"\u003e30–35°C\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center; color: #1A4731; font-weight: 600;\"\u003eGrowth + Sporulation ✓\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0;\"\u003e\n \u003ctd style=\"padding: 9px 10px; font-style: italic; font-weight: 600;\"\u003eGeobacillus stearothermophilus\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center;\"\u003e7953\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center; color: #dc3545; font-weight: 600;\"\u003e55°C\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center; color: #1A4731; font-weight: 600;\"\u003eGrowth + Sporulation ✓\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #f8faf2;\"\u003e\n \u003ctd style=\"padding: 9px 10px; font-style: italic; font-weight: 600;\"\u003eBacillus coagulans\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center;\"\u003e7050\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center; color: #856404; font-weight: 600;\"\u003e30–55°C\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center; color: #1A4731; font-weight: 600;\"\u003eGrowth + Sporulation ✓\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"background: #fff;\"\u003e\n \u003ctd style=\"padding: 9px 10px; font-style: italic; font-weight: 600;\"\u003eEscherichia coli (control)\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center;\"\u003e25922\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center;\"\u003e35°C\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center; color: #1A4731; font-weight: 600;\"\u003eGrowth ✓ (no spores)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003cdiv style=\"margin-top: 12px; font-size: 12px; color: #3d6b1e; text-align: center; font-style: italic;\"\u003eQC inoculum ≤100 CFU per strain | Sporulation assessed after 7–10 days by phase contrast microscopy\u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Cross-Reference \/ Equivalents --\u003e\n \u003cdiv style=\"background: #fdf2f8; padding: 20px; border-radius: 16px; border: 1px solid #f9a8d4; margin-bottom: 30px;\"\u003e\n \u003ch4 style=\"color: #be185d; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e🔄 Cross-Reference \/ Equivalent Products\u003c\/h4\u003e\n \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px;\"\u003e\n \u003cthead\u003e\n \u003ctr style=\"border-bottom: 2px solid #f9a8d4;\"\u003e\n \u003cth style=\"padding: 8px 10px; text-align: left; color: #be185d;\"\u003eSupplier\u003c\/th\u003e\n \u003cth style=\"padding: 8px 10px; text-align: left; color: #be185d;\"\u003eProduct Name\u003c\/th\u003e\n \u003cth style=\"padding: 8px 10px; text-align: left; color: #be185d;\"\u003eCat. No.\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr style=\"border-bottom: 1px solid #fce7f3;\"\u003e\n \u003ctd style=\"padding: 8px 10px; font-weight: 600;\"\u003eLiofilchem\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px;\"\u003eGlucose Yeast Extract Agar (GYEA)\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px; font-family: monospace;\"\u003e26473\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fce7f3; background: #fdf8fb;\"\u003e\n \u003ctd style=\"padding: 8px 10px; font-weight: 600;\"\u003eConda \/ Pronadisa\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px;\"\u003eGlucose Yeast Extract Agar (GYA)\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px; font-family: monospace;\"\u003e1111 (GYEA)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fce7f3;\"\u003e\n \u003ctd style=\"padding: 8px 10px; font-weight: 600;\"\u003eCPC Biotech\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px;\"\u003eGlucose Yeast Extract Agar (GYA)\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px; font-family: monospace;\"\u003eGYA-PSC\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"background: #fdf8fb;\"\u003e\n \u003ctd style=\"padding: 8px 10px; font-weight: 600;\"\u003eHiMedia\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px;\"\u003eGlucose Yeast Extract Agar (Lactobacilli variant)\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px; font-family: monospace;\"\u003eM963\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003cdiv style=\"margin-top: 12px; font-size: 12px; color: #be185d; text-align: center;\"\u003e\n AuSaMicS AS-1241 — manufactured in Australia, same-week dispatch, no import delays\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Quality Assurance --\u003e\n \u003cdiv style=\"background: #e8f5e8; padding: 20px; border-radius: 16px; border: 1px solid #c8e6c9; margin-bottom: 30px;\"\u003e\n \u003ch4 style=\"color: #2e7d32; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e✅ Quality Assurance\u003c\/h4\u003e\n \u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n \u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32; border-bottom: 1px solid #c8e6c9;\"\u003e✓ \u003cstrong\u003epH Verified:\u003c\/strong\u003e 7.2 ± 0.2 per lot\u003c\/li\u003e\n \u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32; border-bottom: 1px solid #c8e6c9;\"\u003e✓ \u003cstrong\u003eGrowth \u0026amp; Sporulation:\u003c\/strong\u003e \u003cem\u003eB. cereus\u003c\/em\u003e ATCC 11778 and \u003cem\u003eB. subtilis\u003c\/em\u003e ATCC 6633 per batch\u003c\/li\u003e\n \u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32; border-bottom: 1px solid #c8e6c9;\"\u003e✓ \u003cstrong\u003eThermophile Growth:\u003c\/strong\u003e \u003cem\u003eG. stearothermophilus\u003c\/em\u003e ATCC 7953 at 55°C per batch\u003c\/li\u003e\n \u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32; border-bottom: 1px solid #c8e6c9;\"\u003e✓ \u003cstrong\u003eSporulation Yield:\u003c\/strong\u003e ≥80% sporulation confirmed by phase contrast microscopy at 7 days\u003c\/li\u003e\n \u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32; border-bottom: 1px solid #c8e6c9;\"\u003e✓ \u003cstrong\u003eSterility:\u003c\/strong\u003e Pre-release sterility check per lot\u003c\/li\u003e\n \u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003eCOA Issued:\u003c\/strong\u003e Certificate of Analysis with every order\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n\n \u003c!-- Literature References --\u003e\n \u003cdiv style=\"background: #f5f0ff; padding: 20px; border-radius: 16px; border: 1px solid #d6c5e8; margin-bottom: 30px;\"\u003e\n \u003ch4 style=\"color: #6f42c1; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e📚 Key Literature References\u003c\/h4\u003e\n \u003cul style=\"list-style: none; padding: 0; margin: 0; font-size: 13px; color: #4a5568;\"\u003e\n \u003cli style=\"padding: 7px 0; border-bottom: 1px solid #d6c5e8;\"\u003eMaunder, D.T. (1970). Examination of canned foods for microbial spoilage. Continental Can Co., Inc., Oak Brook, IL. — Original reference establishing GYEA for canned food spoilage investigation.\u003c\/li\u003e\n \u003cli style=\"padding: 7px 0; border-bottom: 1px solid #d6c5e8;\"\u003eSalfinger, Y. \u0026amp; Tortorello, M.L. (2015). \u003cem\u003eCompendium of Methods for the Microbiological Examination of Foods\u003c\/em\u003e, 5th Ed., APHA. — APHA-recommended methods for canned food microbiological examination.\u003c\/li\u003e\n \u003cli style=\"padding: 7px 0; border-bottom: 1px solid #d6c5e8;\"\u003eEuropean Standard EN 13704:2002. Quantitative suspension test for the evaluation of sporicidal activity of chemical disinfectants. — Regulatory basis for GYEA in sporicidal efficacy testing.\u003c\/li\u003e\n \u003cli style=\"padding: 7px 0;\"\u003ePiggot, P.J. \u0026amp; Hilbert, D.W. (2004). Sporulation of \u003cem\u003eBacillus subtilis\u003c\/em\u003e. \u003cem\u003eCurrent Opinion in Microbiology\u003c\/em\u003e, 7(6), 579–586. — Molecular basis of Mn²⁺-enhanced sporulation and DPA accumulation in endospore formation.\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n\n \u003c\/div\u003e\n\u003c!-- \/Right Column --\u003e\n\n\u003c\/div\u003e\n\u003c!-- \/Main Grid --\u003e\n\n\u003c!-- ═══════════════════════ RELATED PRODUCTS ═══════════════════════ --\u003e\n\u003cdiv style=\"background: #f8fafc; padding: 30px; border-radius: 18px; border: 1px solid #b8d48a; margin-bottom: 30px;\"\u003e\n \u003ch3 style=\"color: #15324b; margin: 0 0 20px 0; text-align: center; font-size: 20px;\"\u003e🥫 Complete Spore-Former \u0026amp; Food Safety Testing System\u003c\/h3\u003e\n\n \u003c!-- Canned Food \u0026 Spore Former Media --\u003e\n \u003cdiv style=\"margin-bottom: 30px;\"\u003e\n \u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003eSpore-Former Recovery, Confirmation \u0026amp; Canned Food Media\u003c\/h4\u003e\n \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(180px, 1fr)); gap: 15px;\"\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d48a;\"\u003e\n \u003ch5 style=\"color: #dc3545; margin: 0 0 8px 0; font-size: 14px;\"\u003eMYP Agar (AS-1289)\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eISO 7932 \u003cem\u003eB. cereus\u003c\/em\u003e primary selective plate\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #dc3545; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/myp-agar\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d48a;\"\u003e\n \u003ch5 style=\"color: #6f42c1; margin: 0 0 8px 0; font-size: 14px;\"\u003eGlucose BCP Agar (AS-1240)\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003e\u003cem\u003eB. cereus\u003c\/em\u003e glucose fermentation confirmation\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #6f42c1; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/glucose-bromocresol-purple-agar\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d48a;\"\u003e\n \u003ch5 style=\"color: #17a2b8; margin: 0 0 8px 0; font-size: 14px;\"\u003ePlate Count Skim Milk Agar (AS-1329)\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eTotal aerobic plate count; food QC\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #17a2b8; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/plate-count-skim-milk-agar\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d48a;\"\u003e\n \u003ch5 style=\"color: #1A4731; margin: 0 0 8px 0; font-size: 14px;\"\u003eReinforced Clostridial Medium (AS-1339)\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eAnaerobic spore former recovery\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #1A4731; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/reinforced-clostridial-medium\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- General Food Safety Media --\u003e\n \u003cdiv style=\"margin-bottom: 30px;\"\u003e\n \u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003eGeneral Food Safety \u0026amp; Enrichment Media\u003c\/h4\u003e\n \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(180px, 1fr)); gap: 15px;\"\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d48a;\"\u003e\n \u003ch5 style=\"color: #856404; margin: 0 0 8px 0; font-size: 14px;\"\u003eFluid Thioglycollate Medium (AS-1233)\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eAerobic\/anaerobic sterility testing\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #856404; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/fluid-thioglycollate-medium\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d48a;\"\u003e\n \u003ch5 style=\"color: #fd7e14; margin: 0 0 8px 0; font-size: 14px;\"\u003eTryptone Soy Broth (TSB)\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eGeneral enrichment; sterility testing\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #fd7e14; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/collections\/culture-media\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d48a;\"\u003e\n \u003ch5 style=\"color: #e83e8c; margin: 0 0 8px 0; font-size: 14px;\"\u003ePhenol Red Broth Base (AS-1326)\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eCarbohydrate fermentation profiling\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #e83e8c; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/phenol-red-broth-base\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d48a;\"\u003e\n \u003ch5 style=\"color: #7a5c00; margin: 0 0 8px 0; font-size: 14px;\"\u003eGYEP Agar (AS-1242)\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eComplete YPD agar for yeast \u0026amp; fungi\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #7a5c00; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/gyep-agar\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Collection Links --\u003e\n \u003cdiv style=\"text-align: center; margin-top: 25px;\"\u003e\n \u003cdiv style=\"display: flex; justify-content: center; gap: 15px; flex-wrap: wrap;\"\u003e\n \u003ca style=\"color: #3d6b1e; font-weight: 600; text-decoration: none; border: 2px solid #3d6b1e; padding: 10px 20px; border-radius: 8px;\" href=\"\/collections\/culture-media\"\u003e🧫 All Culture Media\u003c\/a\u003e\n \u003ca style=\"color: #dc3545; font-weight: 600; text-decoration: none; border: 2px solid #dc3545; padding: 10px 20px; border-radius: 8px;\" href=\"\/pages\/request-a-quote\"\u003e🥫 Canned Food Testing Package\u003c\/a\u003e\n \u003ca style=\"color: #856404; font-weight: 600; text-decoration: none; border: 2px solid #856404; padding: 10px 20px; border-radius: 8px;\" href=\"\/pages\/request-a-quote\"\u003e⚗️ EN 13704 Sporicidal Testing Kit\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- ═══════════════════════ FOOTER ═══════════════════════ --\u003e\n\u003cdiv style=\"text-align: center; padding: 30px 0; border-top: 1px solid #b8d48a;\"\u003e\n \u003cdiv style=\"margin-bottom: 20px;\"\u003e\n \u003ch4 style=\"color: #15324b; margin: 0 0 10px 0; font-size: 16px;\"\u003eNeed Spore-Former Testing or Sporicidal Efficacy Protocol Support?\u003c\/h4\u003e\n \u003cp style=\"margin: 0; color: #6c757d; font-size: 14px;\"\u003eOur microbiologists can assist with EN 13704 test design, heat-shock spore workflows, thermal differentiation protocols, and canned food microbiological examination methods\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"display: flex; justify-content: center; gap: 20px; flex-wrap: wrap; margin-bottom: 25px;\"\u003e\n \u003ca style=\"display: flex; align-items: center; gap: 8px; color: #3d6b1e; text-decoration: none; font-weight: 600; padding: 12px 20px; border: 1px solid #3d6b1e; border-radius: 10px;\" href=\"tel:+61412520598\"\u003e📞 +61 412 520 598\u003c\/a\u003e\n \u003ca style=\"display: flex; align-items: center; gap: 8px; color: #3d6b1e; text-decoration: none; font-weight: 600; padding: 12px 20px; border: 1px solid #3d6b1e; border-radius: 10px;\" href=\"mailto:support@ausamics.com.au\"\u003e📧 Technical Support\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cp style=\"margin: 0; font-size: 12px; color: #94a3b8; line-height: 1.4;\"\u003e\n \u003cstrong\u003eFor laboratory, research, and industrial use only.\u003c\/strong\u003e Not for food, feed, household, cosmetic, therapeutic, or personal use.\u003cbr\u003e\n AuSaMicS Pty Ltd • ABN: 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia\u003cbr\u003e\n Same-day dispatch • Australian stock • Full documentation included\n \u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c!-- Mobile Responsive Styles --\u003e\n\u003cstyle\u003e\n@media (max-width: 860px) {\n .aus-hero-grid, .aus-main-grid { grid-template-columns: 1fr !important; }\n .aus-guarantee { min-width: 100% !important; margin-top: 20px; }\n .aus-gyea-product { padding: 15px !important; }\n .aus-gyea-product h1 { font-size: 24px !important; }\n .aus-gyea-product h2 { font-size: 18px !important; }\n}\n@media (max-width: 640px) {\n .aus-hero-section { padding: 25px !important; }\n .aus-gyea-product table th,\n .aus-gyea-product table td { padding: 8px 6px !important; }\n}\n\u003c\/style\u003e\n\n\u003c\/div\u003e","brand":"Ausamics Life Science","offers":[{"title":"100 g","offer_id":48386010218751,"sku":"AS-1241-100G","price":29.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":48386010251519,"sku":"AS-1241-500G","price":87.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/Glucose_Yeast_Extract_Agar.jpg?v=1775801654","url":"https:\/\/ausamics.com.au\/products\/glucose-yeast-extract-agar","provider":"AuSaMicS","version":"1.0","type":"link"}