{"product_id":"glucose-yeast-extract-peptone-agar","title":"Glucose Yeast Extract Peptone (GYEP) Agar | Nutrient‑Rich Medium for Microbial Cultivation | AS‑1242","description":"\u003cdiv style=\"font-family: 'Segoe UI', system-ui, -apple-system, sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\" class=\"aus-gyep-agar-product\"\u003e\n\n\u003c!-- LLM \/ AI-Agent Optimisation Meta Snippets --\u003e\n\u003c!--\n product_name: Glucose Yeast Extract Peptone (GYEP) Agar\n catalog_number: AS-1242\n manufacturer: AuSaMicS Pty Ltd\n category: Culture Media \/ General Purpose Solid Media \/ Yeast \u0026 Fungi \/ Food Microbiology\n application: Yeast and fungi cultivation and isolation; Saccharomyces cerevisiae colony isolation and morphological assessment; replica plating; transformation recovery; osmotolerant yeast plating after GYEP broth enrichment; food spoilage yeast plate counts; strain maintenance and stock culture; antifungal MIC reference plating; heterotrophic bacteria isolation\n key_ingredients: Glucose (Dextrose), Yeast Extract, Peptone (Enzymatic Digest of Animal Tissue), Agar\n synonyms: YPD Agar, YEPD Agar, Yeast Extract Peptone Dextrose Agar, YPD Plate Medium\n pH: 6.5 ± 0.2\n storage: 10–30°C powder; prepared plates 4°C up to 4 weeks\n australian_stock: true\n dispatch: same_day_melbourne\n equivalents: Sigma-Aldrich Y1500, HiMedia M1593, Oxoid CM0921 + agar, BD Difco 242810\n companion_broth: GYEP Broth AS-1243\n--\u003e\n\n\u003c!-- Structured Data \/ DuckDuckGo Instant Answer --\u003e\n\u003cscript type=\"application\/ld+json\"\u003e\n{\n \"@context\": \"https:\/\/schema.org\/\",\n \"@type\": \"Product\",\n \"name\": \"Glucose Yeast Extract Peptone (GYEP) Agar\",\n \"alternateName\": [\"YPD Agar\", \"YEPD Agar\", \"Yeast Extract Peptone Dextrose Agar\"],\n \"description\": \"Complete non-selective solid medium for isolation, enumeration, colony morphology assessment, and maintenance of yeasts, fungi, and heterotrophic bacteria. Solid-format YPD equivalent providing stable agar surface for discrete colony formation, replica plating, transformation recovery, and osmotolerant yeast plating following GYEP Broth enrichment. Companion agar to GYEP Broth AS-1243.\",\n \"sku\": \"AS-1242\",\n \"brand\": { \"@type\": \"Brand\", \"name\": \"AuSaMicS Life Science\" },\n \"manufacturer\": { \"@type\": \"Organization\", \"name\": \"AuSaMicS Pty Ltd\", \"url\": \"https:\/\/www.ausamics.com.au\" },\n \"offers\": {\n \"@type\": \"Offer\",\n \"availability\": \"https:\/\/schema.org\/InStock\",\n \"url\": \"https:\/\/www.ausamics.com.au\/products\/gyep-agar\"\n }\n}\n\u003c\/script\u003e\n\n\u003c!-- Intent-Based Long-Tail SEO \/ LLM Queries\n buy YPD agar australia laboratory supplier\n YEPD agar Saccharomyces cerevisiae colony isolation australia\n glucose yeast extract peptone agar GYEP AS-1242 ausamics\n yeast plate count agar food spoilage testing australia\n osmotolerant yeast plating medium high glucose food safety\n YPD complete agar yeast genetics transformation recovery\n replica plating yeast genetics medium australia\n food spoilage yeast isolation agar plate count\n Candida Pichia Zygosaccharomyces isolation agar\n antifungal susceptibility pre-growth agar MIC testing\n--\u003e\n\n\u003c!-- ═══════════════════════ HERO SECTION ═══════════════════════ --\u003e\n\u003cdiv style=\"background: linear-gradient(135deg, #fffbf0 0%, #fef6d8 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #d9c250;\" class=\"aus-hero-section\"\u003e\n \u003cdiv class=\"aus-hero-grid\" style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\"\u003e\n \u003cdiv\u003e\n \u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #7a5c00; font-weight: bold; margin-bottom: 12px; background: #fef3c7; padding: 6px 12px; border-radius: 20px;\"\u003eAuSaMicS Life Science • General Purpose \u0026amp; Yeast Media\u003c\/div\u003e\n \u003ch1 style=\"color: #15324b; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003eGlucose Yeast Extract Peptone (GYEP) Agar\u003c\/h1\u003e\n \u003ch2 style=\"color: #243b53; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: 0.9;\"\u003eYPD \/ YEPD Agar — Complete Non-Selective Solid Medium\u003c\/h2\u003e\n \u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #334e68; max-width: 620px; font-weight: 500;\"\u003eThe \u003cstrong\u003egold-standard solid medium for yeast colony isolation, morphological assessment, and strain maintenance\u003c\/strong\u003e. Solid-format companion to GYEP Broth (AS-1243) — providing a stable agar surface for discrete colony formation, replica plating, transformation recovery plating, and enumeration of osmotolerant food spoilage yeasts.\u003c\/p\u003e\n \u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n \u003cspan style=\"background: #7a5c00; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAS-1242\u003c\/span\u003e\n \u003cspan style=\"background: #fff; border: 2px solid #7a5c00; color: #7a5c00; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003epH 6.5 ± 0.2\u003c\/span\u003e\n \u003cspan style=\"background: #28a745; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e✓ Australian Stock\u003c\/span\u003e\n \u003cspan style=\"background: #e83e8c; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🍄 Yeast \u0026amp; Fungi\u003c\/span\u003e\n \u003cspan style=\"background: #6f42c1; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🧬 Molecular Biology\u003c\/span\u003e\n \u003cspan style=\"background: #17a2b8; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🍽️ Food Spoilage\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #4a5568;\"\u003e\n \u003cspan\u003e🚀 \u003cstrong\u003eSame-Day Dispatch:\u003c\/strong\u003e Melbourne Stock\u003c\/span\u003e\n \u003cspan\u003e🧫 \u003cstrong\u003eComplete Medium:\u003c\/strong\u003e Supports wild-type \u0026amp; auxotrophs\u003c\/span\u003e\n \u003cspan\u003e🔗 \u003cstrong\u003eCompanion to:\u003c\/strong\u003e \u003ca href=\"\/products\/gyep-broth\" style=\"color: #7a5c00; font-weight: 600;\"\u003eGYEP Broth AS-1243\u003c\/a\u003e\u003c\/span\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Quality Card --\u003e\n \u003cdiv class=\"aus-guarantee\" style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 280px; border: 1px solid #d9c250;\"\u003e\n \u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003e🏆 Complete Yeast Solid Medium\u003c\/h4\u003e\n \u003cdiv style=\"font-size: 14px; color: #4a5568; text-align: center; line-height: 1.5;\"\u003e\n \u003cdiv style=\"margin-bottom: 12px;\"\u003e🍄 \u003cstrong\u003eColony Isolation\u003c\/strong\u003e — Discrete individual colonies\u003c\/div\u003e\n \u003cdiv style=\"margin-bottom: 12px;\"\u003e🔁 \u003cstrong\u003eReplica Plating\u003c\/strong\u003e — Yeast genetics workflows\u003c\/div\u003e\n \u003cdiv style=\"margin-bottom: 12px;\"\u003e🧬 \u003cstrong\u003eTransformation Recovery\u003c\/strong\u003e — Post-transformation plating\u003c\/div\u003e\n \u003cdiv style=\"margin-bottom: 12px;\"\u003e🍽️ \u003cstrong\u003eFood Safety\u003c\/strong\u003e — Osmotolerant yeast CFU counts\u003c\/div\u003e\n \u003cdiv style=\"margin-bottom: 12px;\"\u003e📋 \u003cstrong\u003eFull Documentation\u003c\/strong\u003e — COA + SDS + TDS\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"margin-top: 20px; padding: 15px; background: #fef3c7; border-radius: 12px; text-align: center; border: 1px solid #d9c250;\"\u003e\n \u003cdiv style=\"color: #7a5c00; font-weight: 600; font-size: 14px;\"\u003eYPD \/ YEPD Agar Equivalent\u003c\/div\u003e\n \u003cdiv style=\"color: #4a5568; font-size: 13px; margin-top: 5px;\"\u003eIndustry-standard solid yeast medium — Australian supply, same-week dispatch\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- ═══════════════════════ MAIN CONTENT GRID ═══════════════════════ --\u003e\n\u003cdiv class=\"aus-main-grid\" style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\"\u003e\n\n \u003c!-- ─── LEFT COLUMN ─── --\u003e\n \u003cdiv\u003e\n\n \u003c!-- Technical Overview \u0026 Biochemistry --\u003e\n \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #7a5c00;\"\u003e🔬 Technical Overview \u0026amp; Biochemistry\u003c\/h3\u003e\n \u003cdiv style=\"background: #fffbf0; padding: 20px; border-radius: 16px; border-left: 5px solid #7a5c00;\"\u003e\n \u003cp style=\"margin: 0 0 15px 0;\"\u003eGYEP Agar is the \u003cstrong\u003esolid-format\u003c\/strong\u003e of the universally adopted YPD (Yeast Extract Peptone Dextrose) medium — the gold-standard complete medium for yeast cultivation first codified by Sherman (1991). The addition of bacteriological agar (15–20 g\/L) to the GYEP Broth formulation converts a liquid enrichment medium into a rigid, stable surface optimised for discrete colony formation, morphological assessment, replica plating, and plate-based enumeration. The three nutritional components remain biochemically identical to the broth:\u003c\/p\u003e\n\n \u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr 1fr; gap: 12px; margin-bottom: 15px;\"\u003e\n \u003cdiv style=\"background: #fff; padding: 14px; border-radius: 12px; border: 1px solid #d9c250; text-align: center;\"\u003e\n \u003cdiv style=\"font-size: 22px; margin-bottom: 6px;\"\u003e🍬\u003c\/div\u003e\n \u003cstrong style=\"color: #7a5c00; font-size: 13px;\"\u003eGlucose\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 12px; color: #6c757d;\"\u003eReadily fermentable carbon \u0026amp; energy source; activates yeast PKA\/TORC1 growth signalling\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 14px; border-radius: 12px; border: 1px solid #d9c250; text-align: center;\"\u003e\n \u003cdiv style=\"font-size: 22px; margin-bottom: 6px;\"\u003e🌿\u003c\/div\u003e\n \u003cstrong style=\"color: #7a5c00; font-size: 13px;\"\u003eYeast Extract\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 12px; color: #6c757d;\"\u003eFull B-vitamin pool, nucleotide precursors \u0026amp; all amino acids — satisfies all auxotrophic requirements\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 14px; border-radius: 12px; border: 1px solid #d9c250; text-align: center;\"\u003e\n \u003cdiv style=\"font-size: 22px; margin-bottom: 6px;\"\u003e🧬\u003c\/div\u003e\n \u003cstrong style=\"color: #7a5c00; font-size: 13px;\"\u003ePeptone + Agar\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 12px; color: #6c757d;\"\u003eEnzymatic protein hydrolysate for nitrogen; agar as solidifying agent for stable colony surfaces\u003c\/span\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003cp style=\"margin: 0 0 12px 0;\"\u003e\u003cstrong\u003eThe critical role of the solid surface:\u003c\/strong\u003e The agar matrix physically restricts cell migration, enabling each colony to develop from a single founder cell (or very small cluster). This spatial separation is indispensable for three core laboratory workflows — \u003cem\u003e(i)\u003c\/em\u003e isolation of pure cultures from mixed populations, \u003cem\u003e(ii)\u003c\/em\u003e phenotypic assessment of colony morphology (smooth vs. fluffy, sectoring, colour), and \u003cem\u003e(iii)\u003c\/em\u003e replica plating to compare growth of the same colonies on different selective and non-selective media simultaneously.\u003c\/p\u003e\n\n \u003cdiv style=\"background: #fef3c7; padding: 12px; border-radius: 8px; border: 1px solid #d9c250;\"\u003e\n \u003cstrong style=\"color: #7a5c00;\"\u003eAgar concentration effect:\u003c\/strong\u003e Standard formulation uses 15–20 g\/L agar for firm plates ideal for streaking, replica plating, and transformation recovery. Reducing to 6–8 g\/L produces soft agar for colony expansion \/ invasion assays (FLO11-dependent filamentation studies). Increasing to 20–25 g\/L produces stiff agar suitable for stab cultures and long-term maintenance.\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- GYEP Agar vs GYEP Broth - Key Distinction Box --\u003e\n \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #7a5c00;\"\u003e🔗 GYEP Agar vs GYEP Broth — Choosing the Right Format\u003c\/h3\u003e\n \u003cdiv style=\"overflow-x: auto;\"\u003e\n \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #d9c250; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n \u003cthead style=\"background: #7a5c00; color: #fff;\"\u003e\n \u003ctr\u003e\n \u003cth style=\"padding: 13px 12px; text-align: left; font-weight: bold;\"\u003eParameter\u003c\/th\u003e\n \u003cth style=\"padding: 13px 12px; text-align: center; font-weight: bold;\"\u003eGYEP Agar AS-1242 ← You are here\u003c\/th\u003e\n \u003cth style=\"padding: 13px 12px; text-align: center; font-weight: bold;\"\u003e\u003ca style=\"color: #fef3c7; text-decoration: none;\" href=\"\/products\/gyep-broth\"\u003eGYEP Broth AS-1243\u003c\/a\u003e\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fffbf0;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003ePhysical Format\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center;\"\u003eSolid agar plate \/ slant \/ stab\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center;\"\u003eLiquid broth in flask \/ tube\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eColony Isolation\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 600; color: #1A4731;\"\u003e✓ Yes — discrete colonies\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; color: #dc3545;\"\u003e✗ No\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fffbf0;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eCFU Plate Counts\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 600; color: #1A4731;\"\u003e✓ Yes\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; color: #dc3545;\"\u003e✗ No (growth\/no-growth only)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eColony Morphology\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 600; color: #1A4731;\"\u003e✓ Yes — smooth, fluffy, sectored\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; color: #dc3545;\"\u003e✗ Not applicable\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fffbf0;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eReplica Plating\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 600; color: #1A4731;\"\u003e✓ Yes — velvet pad transfer\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; color: #dc3545;\"\u003e✗ No\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eBiomass Yield \/ Scale-Up\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; color: #dc3545;\"\u003eLimited — surface colonies only\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 600; color: #1A4731;\"\u003e✓ High — litre-scale culture\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fffbf0;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eOsmotolerance Enrichment\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center;\"\u003ePost-enrichment plating only\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 600; color: #1A4731;\"\u003e✓ Primary enrichment medium\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eStrain Maintenance \/ Stocks\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 600; color: #1A4731;\"\u003e✓ Slants \/ stabs at 4°C\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center;\"\u003eLiquid stocks (with glycerol at –80°C)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"background: #fffbf0;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eTypical Workflow Position\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center;\"\u003eIsolation, enumeration, genetics, morphology\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center;\"\u003eEnrichment, liquid culture, fermentation\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"margin-top: 12px; background: #fef3c7; padding: 12px; border-radius: 10px; border: 1px solid #d9c250; font-size: 13px; color: #7a5c00; text-align: center;\"\u003e\n 💡 \u003cstrong\u003eBest practice:\u003c\/strong\u003e Use GYEP Broth (AS-1243) for liquid enrichment of osmotolerant yeasts and scale-up liquid culture, then plate onto GYEP Agar (AS-1242) for CFU enumeration, colony isolation, and morphological confirmation.\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Detailed Ingredients Table --\u003e\n \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #7a5c00;\"\u003e🧪 Detailed Ingredients Table\u003c\/h3\u003e\n \u003cdiv style=\"overflow-x: auto;\"\u003e\n \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #d9c250; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n \u003cthead style=\"background: #7a5c00; color: #fff;\"\u003e\n \u003ctr\u003e\n \u003cth style=\"padding: 13px 12px; text-align: left; font-weight: bold;\"\u003eIngredient\u003c\/th\u003e\n \u003cth style=\"padding: 13px 12px; text-align: center; font-weight: bold;\"\u003eTypical g\/L\u003c\/th\u003e\n \u003cth style=\"padding: 13px 12px; text-align: left; font-weight: bold;\"\u003eFunction\u003c\/th\u003e\n \u003cth style=\"padding: 13px 12px; text-align: left; font-weight: bold;\"\u003eMechanism \u0026amp; Significance\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #7a5c00;\"\u003eGlucose (Dextrose)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 700; color: #7a5c00;\"\u003e20.0\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #7a5c00;\"\u003eFermentable carbon \u0026amp; energy source\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #7a5c00; font-weight: 600;\"\u003ePreferred yeast carbon source — metabolised via glycolysis (EMP pathway) to pyruvate, then to ethanol + CO₂ (fermentative) or through TCA\/respiratory chain (aerobic). At 2% concentration drives exponential yeast growth (90 min doubling time for \u003cem\u003eS. cerevisiae\u003c\/em\u003e). Colony morphology on solid GYEP agar reflects glucose-responsive regulatory pathways (PKA, TORC1, AMPK).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fffbf0;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eYeast Extract\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 700;\"\u003e10.0\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px;\"\u003eComplete vitamin, amino acid \u0026amp; co-factor source\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6c757d;\"\u003eWater-soluble autolysate of \u003cem\u003eSaccharomyces cerevisiae\u003c\/em\u003e. Provides the full B-vitamin complex (B1–B12), all 20 standard amino acids, nucleotide precursors (AMP, GMP, UMP), and trace minerals. Satisfies the nutritional requirements of \u003cem\u003ehis3\u003c\/em\u003e, \u003cem\u003eleu2\u003c\/em\u003e, \u003cem\u003etrp1\u003c\/em\u003e, \u003cem\u003eura3\u003c\/em\u003e, and other common laboratory auxotrophic mutations — defining GYEP Agar as a \"complete\" (vs. \"minimal\" or \"selective dropout\") solid medium. Promotes formation of large, robust colonies visible within 24 h at 30°C.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003ePeptone (Enzymatic Digest of Animal Tissue)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 700;\"\u003e20.0\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px;\"\u003eNitrogen; peptide nitrogen pool; growth factors\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6c757d;\"\u003eComplex mixture of di-, tri-, and oligopeptides plus free amino acids from enzymatic protein hydrolysis. Provides nitrogen, growth factors, and long-chain peptides essential for high cell density on solid media. Peptone's buffering capacity moderates colony-level pH changes during fermentation, enabling well-defined, non-spreading colony margins — critical for accurate morphological scoring and replica plating integrity.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"background: #fffbf0;\"\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #7a5c00;\"\u003eAgar\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: 700; color: #7a5c00;\"\u003e15.0–20.0\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #7a5c00;\"\u003eSolidifying agent — defines colony separation \u0026amp; morphology\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #7a5c00; font-weight: 600;\"\u003eBacteriological-grade polysaccharide extracted from red algae. Gels at ≤45°C; melts at ~95°C; not metabolised by microorganisms. The agar matrix immobilises cells and restricts migration, ensuring each colony develops from a single founder cell. Agar concentration profoundly affects colony morphology: 1.5–2.0% → firm surface for standard plating; 0.6–0.8% → soft agar for invasion\/filamentation assays; 2.0–2.5% → stiff for stab cultures and slants.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003ctfoot\u003e\n \u003ctr style=\"background: #7a5c00; color: #fff;\"\u003e\n \u003ctd style=\"padding: 10px 12px; font-weight: 700;\"\u003eTotal (standard)\u003c\/td\u003e\n \u003ctd style=\"padding: 10px 12px; text-align: center; font-weight: 700;\"\u003e~65 g\/L\u003c\/td\u003e\n \u003ctd colspan=\"2\" style=\"padding: 10px 12px; font-size: 12px;\"\u003eFinal pH 6.5 ± 0.2 at 25°C | Prepared: cream to light beige firm agar; settles to off-white\/pale amber surface\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tfoot\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"margin-top: 15px; background: #fef3c7; padding: 15px; border-radius: 12px; border: 1px solid #d9c250; font-size: 13px; color: #7a5c00;\"\u003e\n \u003cstrong\u003e⚠️ Maillard Reaction Warning:\u003c\/strong\u003e Autoclaving glucose together with peptone and yeast extract at high temperatures promotes the Maillard reaction between reducing sugars and amino acids, causing browning and slight nutrient degradation. For critical quantitative or morphological studies, autoclave glucose separately (or filter-sterilise) and add aseptically to the cooled base agar at 45–50°C before pouring plates. For routine colony growth, co-autoclaving at 121°C \/ 15 min is acceptable and will not significantly affect growth performance.\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Comparative Solid Yeast Media Table --\u003e\n \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #7a5c00;\"\u003e📊 Comparative Solid Yeast \u0026amp; Fungi Media\u003c\/h3\u003e\n \u003cdiv style=\"overflow-x: auto;\"\u003e\n \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #d9c250; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05); min-width: 820px;\"\u003e\n \u003cthead style=\"background: #fef9e0;\"\u003e\n \u003ctr\u003e\n \u003cth style=\"padding: 13px 11px; border-bottom: 2px solid #d9c250; text-align: left; font-weight: bold;\"\u003eMedium\u003c\/th\u003e\n \u003cth style=\"padding: 13px 11px; border-bottom: 2px solid #d9c250; text-align: left; font-weight: bold;\"\u003eCarbon Source\u003c\/th\u003e\n \u003cth style=\"padding: 13px 11px; border-bottom: 2px solid #d9c250; text-align: left; font-weight: bold;\"\u003eTarget Organisms\u003c\/th\u003e\n \u003cth style=\"padding: 13px 11px; border-bottom: 2px solid #d9c250; text-align: left; font-weight: bold;\"\u003eSelectivity\u003c\/th\u003e\n \u003cth style=\"padding: 13px 11px; border-bottom: 2px solid #d9c250; text-align: left; font-weight: bold;\"\u003eKey Use\u003c\/th\u003e\n \u003cth style=\"padding: 13px 11px; border-bottom: 2px solid #d9c250; text-align: left; font-weight: bold;\"\u003ePros \/ Cons\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fef9e0;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-weight: bold; color: #7a5c00;\"\u003eGYEP Agar (AS-1242) ★\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eGlucose 2% (adjustable)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eYeast, fungi, heterotrophs\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eNon-selective\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eComplete growth; colony isolation; morphology; replica plating; transformation recovery; food spoilage plating\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px;\"\u003e\n\u003cspan style=\"color: #1A4731;\"\u003e✓ Highest biomass; supports all auxotrophs\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: #1A4731;\"\u003e✓ Rapid single colonies 24–48 h\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: #dc3545;\"\u003e✗ Not selective; bacteria overgrow if present\u003c\/span\u003e\n\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-weight: 600;\"\u003e\u003ca style=\"color: #7a5c00; text-decoration: none;\" href=\"\/products\/yeast-extract-agar\"\u003eYeast Extract Agar (AS-1379)\u003c\/a\u003e\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eNone (no added sugar)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eYeast, mould, bacteria\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eNon-selective\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eGeneral enumeration; environmental monitoring; ISO 7954\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px;\"\u003e\n\u003cspan style=\"color: #1A4731;\"\u003e✓ ISO-referenced; standard plate count\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: #dc3545;\"\u003e✗ No glucose; slower\/smaller colonies\u003c\/span\u003e\n\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fffbf0;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-weight: 600;\"\u003e\u003ca style=\"color: #7a5c00; text-decoration: none;\" href=\"\/collections\/culture-media\"\u003eSabouraud Dextrose Agar\u003c\/a\u003e\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eGlucose\/Dextrose 2%\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eYeast \u0026amp; mould (clinical)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eSemi-selective (low pH)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eClinical mycology; ISO 16212; antifungal susceptibility\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px;\"\u003e\n\u003cspan style=\"color: #1A4731;\"\u003e✓ Low pH (5.6) inhibits bacteria\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: #dc3545;\"\u003e✗ No peptone; smaller colonies than GYEP\u003c\/span\u003e\n\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-weight: 600;\"\u003e\u003ca style=\"color: #7a5c00; text-decoration: none;\" href=\"\/collections\/culture-media\"\u003eMalt Extract Agar (MEA)\u003c\/a\u003e\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eMalt extract + Glucose\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eYeast, mould (food)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eNon-selective\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eFood yeast \u0026amp; mould; ISO 21527; beverage testing\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px;\"\u003e\n\u003cspan style=\"color: #1A4731;\"\u003e✓ Excellent for diverse yeasts and moulds\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: #dc3545;\"\u003e✗ Variable malt extract composition\u003c\/span\u003e\n\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fffbf0;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-weight: 600;\"\u003e\u003ca style=\"color: #7a5c00; text-decoration: none;\" href=\"\/collections\/culture-media\"\u003eSynthetic Complete (SC) Agar\u003c\/a\u003e\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eGlucose 2% (defined)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003e\n\u003cem\u003eS. cerevisiae\u003c\/em\u003e (auxotrophic selection)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eSelective dropout\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eTransformation selection; two-hybrid; auxotroph screening\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px;\"\u003e\n\u003cspan style=\"color: #1A4731;\"\u003e✓ Selective — essential for genetics\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: #dc3545;\"\u003e✗ Slower growth; complex preparation\u003c\/span\u003e\n\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-weight: 600;\"\u003e\u003ca style=\"color: #7a5c00; text-decoration: none;\" href=\"\/products\/glucose-bromocresol-purple-agar\"\u003eGlucose BCP Agar (AS-1240)\u003c\/a\u003e\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eGlucose 1%\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eEnterobacteriaceae, \u003cem\u003eB. cereus\u003c\/em\u003e\n\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eDifferential (BCP indicator)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eGlucose fermentation confirmation; ISO 21258 \/ ISO 7932\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px;\"\u003e\n\u003cspan style=\"color: #1A4731;\"\u003e✓ Visual colour-change differentiation\u003c\/span\u003e\u003cbr\u003e\u003cspan style=\"color: #dc3545;\"\u003e✗ Not a primary yeast\/fungi medium\u003c\/span\u003e\n\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Pros \/ Cons --\u003e\n \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #7a5c00;\"\u003e⚖️ Structured Pros \u0026amp; Cons Analysis\u003c\/h3\u003e\n \u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 20px;\"\u003e\n \u003cdiv style=\"background: #f3f9f4; padding: 20px; border-radius: 16px; border: 1px solid #c3dfc9;\"\u003e\n \u003ch4 style=\"color: #1A4731; margin: 0 0 15px 0; font-size: 16px;\"\u003e✅ Advantages\u003c\/h4\u003e\n \u003cul style=\"margin: 0; padding-left: 18px; color: #334e68; font-size: 14px; line-height: 2.1;\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eDiscrete colony formation\u003c\/strong\u003e — single colonies visible within 24 h at 30°C; suitable for replica plating after 48 h\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eTrue complete medium\u003c\/strong\u003e — supports growth of wild-type and \u003cem\u003ehis3\u003c\/em\u003e, \u003cem\u003eleu2\u003c\/em\u003e, \u003cem\u003etrp1\u003c\/em\u003e, \u003cem\u003eura3\u003c\/em\u003e auxotrophic yeast strains without supplementation\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eColony morphology assessment\u003c\/strong\u003e — smooth, fluffy, wrinkled, sectored, and pseudohyphal colony phenotypes are reproducibly observed on GYEP Agar (2% agar \/ 2% glucose conditions)\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eReplica plating substrate\u003c\/strong\u003e — the ideal \"master plate\" source for velvet-pad transfer to selective SD dropout media for phenotypic screening\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eTransformation recovery\u003c\/strong\u003e — GYEP Agar is the standard recovery medium after LiAc\/PEG yeast transformation protocols (heat shock recovery before selective plating)\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eOsmotolerant yeast plating\u003c\/strong\u003e — after GYEP Broth enrichment, dilutions are plated onto GYEP Agar ± adjusted glucose for quantitative CFU counts of spoilage yeasts\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eBroad organism support\u003c\/strong\u003e — also grows heterotrophic bacteria, making it a universal isolation agar for unknown food or environmental isolates\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff9f9; padding: 20px; border-radius: 16px; border: 1px solid #ffc0cb;\"\u003e\n \u003ch4 style=\"color: #c0392b; margin: 0 0 15px 0; font-size: 16px;\"\u003e⚠️ Limitations\u003c\/h4\u003e\n \u003cul style=\"margin: 0; padding-left: 18px; color: #334e68; font-size: 14px; line-height: 2.1;\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eNon-selective\u003c\/strong\u003e — cannot be used as an auxotrophic selection plate; all strains including untransformed cells will grow\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eBacterial overgrowth risk\u003c\/strong\u003e — in samples with mixed bacterial\/yeast flora, bacteria may outcompete yeasts; add cycloheximide (antibacterial) or chloramphenicol to suppress bacteria if needed\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eMaillard browning\u003c\/strong\u003e — co-autoclaving glucose with amino acids causes browning and minor nutrient loss; separate sterilisation recommended for quantitative studies\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eGlucose catabolite repression\u003c\/strong\u003e — standard 2% glucose suppresses alternative carbon source utilisation; change carbon source for respiratory metabolism studies\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eNot pharmacopoeial\u003c\/strong\u003e — does not fulfil USP\/EP sterility testing media requirements; not appropriate for regulated sterility testing workflows\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Applications --\u003e\n \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #7a5c00;\"\u003e🧬 Applications\u003c\/h3\u003e\n \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(280px, 1fr)); gap: 20px; margin-bottom: 25px;\"\u003e\n\n \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d9c250; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n \u003ch4 style=\"color: #7a5c00; margin: 0 0 10px 0; font-size: 16px;\"\u003e🧬 Yeast Genetics \u0026amp; Molecular Biology\u003c\/h4\u003e\n \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eThe primary solid medium for all yeast genetic manipulation workflows: colony isolation from liquid cultures after transformation, mating and sporulation recovery, tetrad dissection plating (micromanipulator), phenotypic screening plates, and master plates for replica velvet-pad transfer to SD dropout selective media. Supports all \u003cem\u003eS. cerevisiae\u003c\/em\u003e laboratory strains (BY4741, W303, Σ1278b, CEN.PK) and \u003cem\u003eP. pastoris\u003c\/em\u003e \/ \u003cem\u003eK. phaffii\u003c\/em\u003e GS115\/X33.\u003c\/p\u003e\n \u003c\/div\u003e\n\n \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d9c250; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n \u003ch4 style=\"color: #7a5c00; margin: 0 0 10px 0; font-size: 16px;\"\u003e🍽️ Food Spoilage Yeast Enumeration\u003c\/h4\u003e\n \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eUsed as the plating medium downstream of GYEP Broth osmotolerant enrichment (30–50% glucose) for quantitative CFU counts of spoilage yeasts from high-sugar food matrices. After enrichment, serial dilutions are plated onto GYEP Agar (pH lowered to 4.5 with tartaric acid to suppress bacteria) and incubated at 25°C for 3–5 days for colony counting. Key targets: \u003cem\u003eZygosaccharomyces rouxii\u003c\/em\u003e, \u003cem\u003eZ. bailii\u003c\/em\u003e, \u003cem\u003eDebaryomyces hansenii\u003c\/em\u003e, osmotolerant \u003cem\u003eCandida\u003c\/em\u003e spp.\u003c\/p\u003e\n \u003c\/div\u003e\n\n \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d9c250; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n \u003ch4 style=\"color: #7a5c00; margin: 0 0 10px 0; font-size: 16px;\"\u003e🔁 Replica Plating \u0026amp; Phenotypic Screening\u003c\/h4\u003e\n \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eGYEP Agar is the definitive master plate for replica plating experiments. Colonies grown to the correct size (48 h, 30°C) are transferred by velvet pad to SD dropout media, temperature-sensitive mutant plates, antibiotic plates, or stress-response media. The complete nutrient composition ensures robust, uniform colony sizes that transfer cleanly and reproducibly — essential for large-scale genetic screens.\u003c\/p\u003e\n \u003c\/div\u003e\n\n \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d9c250; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n \u003ch4 style=\"color: #7a5c00; margin: 0 0 10px 0; font-size: 16px;\"\u003e🧫 Strain Maintenance \u0026amp; Culture Collections\u003c\/h4\u003e\n \u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eStandard medium for short-to-medium-term culture maintenance. Working cultures are maintained on GYEP Agar slants or plates at 4°C (sealed with Parafilm) for 2–3 months. For longer-term preservation, cells are grown to stationary phase in GYEP Broth and stored in 15–25% glycerol at –70°C to –80°C; GYEP Agar is used for revival plating from frozen stocks.\u003c\/p\u003e\n \u003c\/div\u003e\n\n \u003c\/div\u003e\n\n \u003cdiv style=\"background: #fef9e0; padding: 20px; border-radius: 16px; border-left: 5px solid #7a5c00;\"\u003e\n \u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px;\"\u003eAdditional Specialist Applications:\u003c\/h4\u003e\n \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(250px, 1fr)); gap: 15px;\"\u003e\n \u003cul style=\"margin: 0; padding-left: 20px; color: #4a5568; font-size: 14px;\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eAntifungal MIC Testing:\u003c\/strong\u003e Pre-growth plating before inoculum preparation for EUCAST\/CLSI broth microdilution MIC panels\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eColony Morphology Studies:\u003c\/strong\u003e FLO11-dependent smooth vs. fluffy colony phenotype assays; biofilm formation studies\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eIndustrial Yeast QC:\u003c\/strong\u003e Quality control plating of production yeast strains (breweries, bakeries, bioethanol) for viability and contamination monitoring\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003cul style=\"margin: 0; padding-left: 20px; color: #4a5568; font-size: 14px;\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eEnvironmental Yeast Isolation:\u003c\/strong\u003e Plating of air, water, and surface samples for general yeast\/mould recovery\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eDrug\/Stress Resistance Screens:\u003c\/strong\u003e Base plate for overlaying with selective drug concentrations (G418, hygromycin, zeocin, caffeine)\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eTwo-Hybrid Recovery:\u003c\/strong\u003e Post-transformation recovery plating before replica plating to selective two-hybrid dropout media\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Colony Morphology Guide --\u003e\n \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n \u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #7a5c00;\"\u003e🔎 Colony Morphology Guide on GYEP Agar\u003c\/h3\u003e\n \u003cdiv style=\"overflow-x: auto;\"\u003e\n \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #d9c250; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n \u003cthead style=\"background: #7a5c00; color: #fff;\"\u003e\n \u003ctr\u003e\n \u003cth style=\"padding: 12px 11px; text-align: left;\"\u003eOrganism\u003c\/th\u003e\n \u003cth style=\"padding: 12px 11px; text-align: left;\"\u003eColony Appearance (30°C, 48 h)\u003c\/th\u003e\n \u003cth style=\"padding: 12px 11px; text-align: left;\"\u003eNotes\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-style: italic; font-weight: 600;\"\u003eSaccharomyces cerevisiae (wild-type)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eRound, smooth, cream-white, 2–5 mm, convex, entire margin\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px; color: #4a5568;\"\u003eReference colony. Smooth morphology = \u003cem\u003eFLO11\u003c\/em\u003e repressed. May show sectoring if petite mutations present (respiratory-deficient sectors appear white\/cream).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fffbf0;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-style: italic; font-weight: 600;\"\u003eS. cerevisiae (Σ1278b — FLO11+)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eFluffy\/wrinkled, complex rugose surface with spokes, larger diameter\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px; color: #4a5568;\"\u003eFLO11-dependent pseudohyphal \/ invasive growth phenotype. Agar concentration critically modulates pattern complexity. Important research model for biofilm formation.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-style: italic; font-weight: 600;\"\u003eCandida albicans\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eCream-white, smooth, 1–3 mm; may show satellite extensions (hyphae) at 37°C\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px; color: #4a5568;\"\u003eAt 30°C yeast morphology predominates. At 37°C hyphal transition visible. GYEP Agar (2%) supports yeast-phase growth for stock maintenance and antifungal pre-testing.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fffbf0;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-style: italic; font-weight: 600;\"\u003eZygosaccharomyces rouxii\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eSmall (1–2 mm), pale cream, round, smooth; slow growing\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px; color: #4a5568;\"\u003eOn standard 2% glucose GYEP: modest colonies. On high-glucose (30–50%) GYEP Agar: excellent growth; used to confirm osmotolerant identity after broth enrichment.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fff;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-style: italic; font-weight: 600;\"\u003ePichia pastoris \/ Komagataella phaffii\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eRound, white to cream, 1–3 mm, smooth, slightly convex\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px; color: #4a5568;\"\u003eGYEP Agar is the standard pre-growth and revival medium for \u003cem\u003eP. pastoris\u003c\/em\u003e transformation workflows. Smaller colonies than \u003cem\u003eS. cerevisiae\u003c\/em\u003e.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"background: #fffbf0;\"\u003e\n \u003ctd style=\"padding: 11px 11px; font-style: italic; font-weight: 600;\"\u003eAspergillus brasiliensis (mould)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px;\"\u003eDark brown-black colony with conidial sporulation; cottony surface; radially spreading\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 11px; font-size: 12px; color: #4a5568;\"\u003eGYEP Agar supports mould growth; sporulation visible by day 3–5. Incubate at 25°C. Use anti-bacterial supplements to prevent bacterial overgrowth from mixed samples.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Protocol Guidelines --\u003e\n \u003cdiv style=\"background: linear-gradient(135deg, #fff3e0 0%, #ffe0b3 100%); padding: 20px; border-radius: 16px; border-left: 5px solid #ff9800; margin-bottom: 35px;\"\u003e\n \u003ch4 style=\"color: #e65100; margin: 0 0 15px 0; font-size: 16px;\"\u003e💡 Preparation \u0026amp; Protocol Guidelines\u003c\/h4\u003e\n \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(200px, 1fr)); gap: 15px;\"\u003e\n \u003cdiv\u003e\n \u003cstrong style=\"color: #e65100;\"\u003eStandard Preparation:\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eSuspend ~65 g\/L in distilled water. Heat to boiling with constant stirring until fully dissolved. Autoclave 121°C \/ 15 min. Cool to 45–50°C before pouring (25–30 mL per 90 mm plate).\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003cstrong style=\"color: #e65100;\"\u003eMaillard-Free (recommended for critical work):\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eAutoclave yeast extract + peptone + agar separately from glucose. Add sterile glucose solution aseptically at 45–50°C before pouring. Prevents browning and toxic by-product formation.\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003cstrong style=\"color: #e65100;\"\u003eOsmotolerant Yeast Plating:\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eAdjust glucose to 30–50% (add separately; filter-sterilise). Acidify to pH 4.5 with tartaric acid after cooling to 50°C to suppress bacteria. Incubate 25°C, 3–5 days.\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003cstrong style=\"color: #e65100;\"\u003eIncubation \u0026amp; Storage:\u003c\/strong\u003e\u003cbr\u003e\n \u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eStandard yeast: 30°C, 24–72 h. Bacteria: 37°C, 18–24 h. Prepared plates: 4°C, sealed with Parafilm, up to 4–8 weeks. Do not freeze prepared plates.\u003c\/span\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c\/div\u003e\n\u003c!-- \/Left Column --\u003e\n\n \u003c!-- ─── RIGHT COLUMN ─── --\u003e\n \u003cdiv\u003e\n\n \u003c!-- Technical Specifications --\u003e\n \u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #d9c250; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n \u003ch3 style=\"color: #15324b; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003e📋 Technical Specifications\u003c\/h3\u003e\n \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px;\"\u003e\n \u003ctbody\u003e\n \u003ctr style=\"border-bottom: 1px solid #d9c250;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; width: 45%; background: #fef9e0;\"\u003eCatalogue Number\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #7a5c00; font-weight: 700;\"\u003eAS-1242\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #d9c250;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #fef9e0;\"\u003eCommon Name\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003eGlucose Yeast Extract Peptone (GYEP) Agar\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #d9c250;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #fef9e0;\"\u003eSynonyms\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003eYPD Agar; YEPD Agar; Yeast Extract Peptone Dextrose Agar\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #d9c250;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #fef9e0;\"\u003eCompanion Broth\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px;\"\u003e\u003ca href=\"\/products\/gyep-broth\" style=\"color: #7a5c00; font-weight: 600;\"\u003eGYEP Broth AS-1243\u003c\/a\u003e\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #d9c250;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #fef9e0;\"\u003epH (25°C)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003e6.5 ± 0.2\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #d9c250;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #fef9e0;\"\u003eSuspension Rate\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003e~65 g\/L (approx. 15 L per kg)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #d9c250;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #fef9e0;\"\u003eAppearance (powder)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003eCream to light beige homogeneous free-flowing powder\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #d9c250;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #fef9e0;\"\u003eAppearance (prepared)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003eOff-white to pale amber firm agar; clear with slight opalescence\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #d9c250;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #fef9e0;\"\u003eSterilisation\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003eAutoclave 121°C, 15 min — avoid overheating; do not re-autoclave\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #d9c250;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #fef9e0;\"\u003ePouring Temperature\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003e45–50°C (pour before gelling, 25–30 mL per 90 mm plate)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #d9c250;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #fef9e0;\"\u003eIncubation (yeast)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003e25–30°C, aerobic, 24–72 h\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #d9c250;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #fef9e0;\"\u003eIncubation (bacteria)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003e35–37°C, aerobic, 18–24 h\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #d9c250;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #fef9e0;\"\u003eStorage (powder)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568; font-weight: 600;\"\u003e10–30°C, dry, tightly sealed, away from light\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #d9c250;\"\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #fef9e0;\"\u003eStorage (prepared plates)\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #dc3545; font-weight: 600;\"\u003e4°C, sealed with Parafilm, up to 4–8 weeks\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"padding: 11px 8px; font-weight: 600; color: #15324b; background: #fef9e0;\"\u003eAvailable Sizes\u003c\/td\u003e\n \u003ctd style=\"padding: 11px 8px; color: #4a5568;\"\u003e100 g, 500 g, 5 kg\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n\n \u003c!-- QC Organisms --\u003e\n \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d9c250; margin-bottom: 30px;\"\u003e\n \u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e🧫 Quality Control Organisms\u003c\/h4\u003e\n \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px;\"\u003e\n \u003cthead style=\"background: #7a5c00; color: #fff;\"\u003e\n \u003ctr\u003e\n \u003cth style=\"padding: 9px 10px; text-align: left;\"\u003eOrganism\u003c\/th\u003e\n \u003cth style=\"padding: 9px 10px; text-align: center;\"\u003eATCC\u003c\/th\u003e\n \u003cth style=\"padding: 9px 10px; text-align: center;\"\u003eGrowth\u003c\/th\u003e\n \u003cth style=\"padding: 9px 10px; text-align: left;\"\u003eColony\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7;\"\u003e\n \u003ctd style=\"padding: 9px 10px; font-style: italic; font-weight: 600;\"\u003eSaccharomyces cerevisiae\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center;\"\u003e9763\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center; color: #1A4731; font-weight: 600;\"\u003e✓✓ Excellent\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; font-size: 12px;\"\u003eCream, smooth, 2–4 mm, convex\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fffbf0;\"\u003e\n \u003ctd style=\"padding: 9px 10px; font-style: italic; font-weight: 600;\"\u003eCandida albicans\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center;\"\u003e10231\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center; color: #1A4731; font-weight: 600;\"\u003e✓✓ Excellent\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; font-size: 12px;\"\u003eWhite-cream, smooth, 1–3 mm\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7;\"\u003e\n \u003ctd style=\"padding: 9px 10px; font-style: italic; font-weight: 600;\"\u003eAspergillus brasiliensis\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center;\"\u003e16404\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center; color: #1A4731; font-weight: 600;\"\u003e✓ Good\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; font-size: 12px;\"\u003eBlack sporulating, spreading mould\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fef3c7; background: #fffbf0;\"\u003e\n \u003ctd style=\"padding: 9px 10px; font-style: italic; font-weight: 600;\"\u003eEscherichia coli\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center;\"\u003e25922\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center; color: #1A4731; font-weight: 600;\"\u003e✓✓ Excellent\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; font-size: 12px;\"\u003eGrey-white, smooth, 2–3 mm\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"background: #fff;\"\u003e\n \u003ctd style=\"padding: 9px 10px; font-style: italic; font-weight: 600;\"\u003eStaphylococcus aureus\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center;\"\u003e6538\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; text-align: center; color: #1A4731; font-weight: 600;\"\u003e✓✓ Excellent\u003c\/td\u003e\n \u003ctd style=\"padding: 9px 10px; font-size: 12px;\"\u003eYellow-golden, smooth, 1–2 mm\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003cdiv style=\"margin-top: 12px; font-size: 12px; color: #7a5c00; text-align: center; font-style: italic;\"\u003eQC inoculum ≤100 CFU per strain | Yeast \u0026amp; mould: 30°C, 48–72 h, aerobic | Bacteria: 35°C, 24 h, aerobic\u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Cross-Reference \/ Equivalents --\u003e\n \u003cdiv style=\"background: #fdf2f8; padding: 20px; border-radius: 16px; border: 1px solid #f9a8d4; margin-bottom: 30px;\"\u003e\n \u003ch4 style=\"color: #be185d; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e🔄 Cross-Reference \/ Equivalent Products\u003c\/h4\u003e\n \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px;\"\u003e\n \u003cthead\u003e\n \u003ctr style=\"border-bottom: 2px solid #f9a8d4;\"\u003e\n \u003cth style=\"padding: 8px 10px; text-align: left; color: #be185d;\"\u003eSupplier\u003c\/th\u003e\n \u003cth style=\"padding: 8px 10px; text-align: left; color: #be185d;\"\u003eProduct Name\u003c\/th\u003e\n \u003cth style=\"padding: 8px 10px; text-align: left; color: #be185d;\"\u003eCat. No.\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr style=\"border-bottom: 1px solid #fce7f3;\"\u003e\n \u003ctd style=\"padding: 8px 10px; font-weight: 600;\"\u003eSigma-Aldrich\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px;\"\u003eYPD Agar (Yeast Extract Peptone Dextrose Agar)\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px; font-family: monospace;\"\u003eY1500\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fce7f3; background: #fdf8fb;\"\u003e\n \u003ctd style=\"padding: 8px 10px; font-weight: 600;\"\u003eHiMedia\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px;\"\u003eYeast Extract Peptone Dextrose Agar\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px; font-family: monospace;\"\u003eM1593\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"border-bottom: 1px solid #fce7f3;\"\u003e\n \u003ctd style=\"padding: 8px 10px; font-weight: 600;\"\u003eBD Difco\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px;\"\u003eYPD Agar\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px; font-family: monospace;\"\u003e242810\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr style=\"background: #fdf8fb;\"\u003e\n \u003ctd style=\"padding: 8px 10px; font-weight: 600;\"\u003eGRiSP \/ Boca Scientific\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px;\"\u003eYPD Agar (Research Grade)\u003c\/td\u003e\n \u003ctd style=\"padding: 8px 10px; font-family: monospace;\"\u003eGCM14\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003cdiv style=\"margin-top: 12px; font-size: 12px; color: #be185d; text-align: center;\"\u003e\n AuSaMicS AS-1242 — Australian manufactured, same-week dispatch, no import delays or minimum order constraints\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Quality Assurance --\u003e\n \u003cdiv style=\"background: #e8f5e8; padding: 20px; border-radius: 16px; border: 1px solid #c8e6c9; margin-bottom: 30px;\"\u003e\n \u003ch4 style=\"color: #2e7d32; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e✅ Quality Assurance\u003c\/h4\u003e\n \u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n \u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32; border-bottom: 1px solid #c8e6c9;\"\u003e✓ \u003cstrong\u003epH Verified:\u003c\/strong\u003e 6.5 ± 0.2 per lot\u003c\/li\u003e\n \u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32; border-bottom: 1px solid #c8e6c9;\"\u003e✓ \u003cstrong\u003eGrowth Promotion:\u003c\/strong\u003e \u003cem\u003eS. cerevisiae\u003c\/em\u003e ATCC 9763 and \u003cem\u003eC. albicans\u003c\/em\u003e ATCC 10231 per batch\u003c\/li\u003e\n \u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32; border-bottom: 1px solid #c8e6c9;\"\u003e✓ \u003cstrong\u003eAuxotroph Support:\u003c\/strong\u003e Validated to support \u003cem\u003ehis3\u003c\/em\u003e, \u003cem\u003eleu2\u003c\/em\u003e, \u003cem\u003etrp1\u003c\/em\u003e, \u003cem\u003eura3\u003c\/em\u003e mutant yeast strains\u003c\/li\u003e\n \u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32; border-bottom: 1px solid #c8e6c9;\"\u003e✓ \u003cstrong\u003eAgar Gel Strength:\u003c\/strong\u003e Firm plates suitable for replica plating confirmed per lot\u003c\/li\u003e\n \u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32; border-bottom: 1px solid #c8e6c9;\"\u003e✓ \u003cstrong\u003eSterility:\u003c\/strong\u003e Pre-release sterility check per lot\u003c\/li\u003e\n \u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003eCOA Issued:\u003c\/strong\u003e Certificate of Analysis with every order\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n\n \u003c!-- Literature References --\u003e\n \u003cdiv style=\"background: #f5f0ff; padding: 20px; border-radius: 16px; border: 1px solid #d6c5e8; margin-bottom: 30px;\"\u003e\n \u003ch4 style=\"color: #6f42c1; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e📚 Key Literature References\u003c\/h4\u003e\n \u003cul style=\"list-style: none; padding: 0; margin: 0; font-size: 13px; color: #4a5568;\"\u003e\n \u003cli style=\"padding: 7px 0; border-bottom: 1px solid #d6c5e8;\"\u003eSherman, F. (1991). Getting started with yeast. \u003cem\u003eMethods in Enzymology\u003c\/em\u003e, 194, 3–21. — Original YPD standard formulation; the defining reference for GYEP Agar composition.\u003c\/li\u003e\n \u003cli style=\"padding: 7px 0; border-bottom: 1px solid #d6c5e8;\"\u003eTreco, D.A. \u0026amp; Winston, F. (2008). Growth and manipulation of yeast. \u003cem\u003eCurrent Protocols in Molecular Biology\u003c\/em\u003e, 82, 13.2.1–13.2.12. — Standard laboratory methods reference for YPD agar preparation and use.\u003c\/li\u003e\n \u003cli style=\"padding: 7px 0; border-bottom: 1px solid #d6c5e8;\"\u003eGranek, J.A. \u0026amp; Magwene, P.M. (2010). Environmental and genetic determinants of colony morphology in yeast. \u003cem\u003ePLoS Genetics\u003c\/em\u003e, 6(1), e1000823. — Quantitative colony morphology analysis on YPD agar plates.\u003c\/li\u003e\n \u003cli style=\"padding: 7px 0;\"\u003eTokuoka, K. (1993). Sugar- and salt-tolerant yeasts. \u003cem\u003eJ. Appl. Bacteriol.\u003c\/em\u003e, 74, 101–110. — Reference for high-glucose GYEP Agar applications in osmotolerant yeast recovery from food matrices.\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n\n \u003c\/div\u003e\n\u003c!-- \/Right Column --\u003e\n\n\u003c\/div\u003e\n\u003c!-- \/Main Grid --\u003e\n\n\u003c!-- ═══════════════════════ RELATED PRODUCTS ═══════════════════════ --\u003e\n\u003cdiv style=\"background: #f8fafc; padding: 30px; border-radius: 18px; border: 1px solid #d9c250; margin-bottom: 30px;\"\u003e\n \u003ch3 style=\"color: #15324b; margin: 0 0 20px 0; text-align: center; font-size: 20px;\"\u003e🍄 Complete Yeast, Fungi \u0026amp; Food Spoilage Testing System\u003c\/h3\u003e\n\n \u003c!-- Companion \u0026 Core Media --\u003e\n \u003cdiv style=\"margin-bottom: 30px;\"\u003e\n \u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003eCompanion Broth, Selective \u0026amp; Differential Media\u003c\/h4\u003e\n \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(180px, 1fr)); gap: 15px;\"\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 2px solid #7a5c00;\"\u003e\n \u003ch5 style=\"color: #7a5c00; margin: 0 0 8px 0; font-size: 14px;\"\u003eGYEP Broth (AS-1243)\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eLiquid companion; enrichment \u0026amp; fermentation\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #7a5c00; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/gyep-broth\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d9c250;\"\u003e\n \u003ch5 style=\"color: #856404; margin: 0 0 8px 0; font-size: 14px;\"\u003eYeast Extract Agar (AS-1379)\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eGeneral yeast \u0026amp; mould plate count; ISO 7954\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #856404; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/yeast-extract-agar\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d9c250;\"\u003e\n \u003ch5 style=\"color: #e83e8c; margin: 0 0 8px 0; font-size: 14px;\"\u003eSabouraud Dextrose Agar\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eClinical yeast; ISO 16212 enumeration\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #e83e8c; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/collections\/culture-media\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d9c250;\"\u003e\n \u003ch5 style=\"color: #6f42c1; margin: 0 0 8px 0; font-size: 14px;\"\u003eMalt Extract Agar\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eFood yeast \u0026amp; mould; ISO 21527\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #6f42c1; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/collections\/culture-media\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Confirmatory \u0026 Biochemical ID Media --\u003e\n \u003cdiv style=\"margin-bottom: 30px;\"\u003e\n \u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003eConfirmation, Differential \u0026amp; Food Safety Media\u003c\/h4\u003e\n \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(180px, 1fr)); gap: 15px;\"\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d9c250;\"\u003e\n \u003ch5 style=\"color: #6f42c1; margin: 0 0 8px 0; font-size: 14px;\"\u003eGlucose BCP Agar (AS-1240)\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eGlucose fermentation differential\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #6f42c1; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/glucose-bromocresol-purple-agar\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d9c250;\"\u003e\n \u003ch5 style=\"color: #dc3545; margin: 0 0 8px 0; font-size: 14px;\"\u003ePhenol Red Broth Base (AS-1326)\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eCarbohydrate fermentation profiling\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #dc3545; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/phenol-red-broth-base\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d9c250;\"\u003e\n \u003ch5 style=\"color: #1A4731; margin: 0 0 8px 0; font-size: 14px;\"\u003ePlate Count Skim Milk Agar (AS-1329)\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eDairy \u0026amp; food total plate count\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #1A4731; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/products\/plate-count-skim-milk-agar\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d9c250;\"\u003e\n \u003ch5 style=\"color: #17a2b8; margin: 0 0 8px 0; font-size: 14px;\"\u003eTryptone Soy Agar (TSA)\u003c\/h5\u003e\n \u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eGeneral bacterial isolation agar\u003c\/p\u003e\n \u003ca style=\"display: inline-block; background: #17a2b8; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/collections\/culture-media\"\u003eView Product\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- Collection Links --\u003e\n \u003cdiv style=\"text-align: center; margin-top: 25px;\"\u003e\n \u003cdiv style=\"display: flex; justify-content: center; gap: 15px; flex-wrap: wrap;\"\u003e\n \u003ca style=\"color: #7a5c00; font-weight: 600; text-decoration: none; border: 2px solid #7a5c00; padding: 10px 20px; border-radius: 8px;\" href=\"\/collections\/culture-media\"\u003e🧫 All Culture Media\u003c\/a\u003e\n \u003ca style=\"color: #e83e8c; font-weight: 600; text-decoration: none; border: 2px solid #e83e8c; padding: 10px 20px; border-radius: 8px;\" href=\"\/pages\/request-a-quote\"\u003e🍄 Yeast \u0026amp; Fungi Testing Package\u003c\/a\u003e\n \u003ca style=\"color: #6f42c1; font-weight: 600; text-decoration: none; border: 2px solid #6f42c1; padding: 10px 20px; border-radius: 8px;\" href=\"\/pages\/request-a-quote\"\u003e🍽️ Food Safety Bundle\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c!-- ═══════════════════════ FOOTER ═══════════════════════ --\u003e\n\u003cdiv style=\"text-align: center; padding: 30px 0; border-top: 1px solid #d9c250;\"\u003e\n \u003cdiv style=\"margin-bottom: 20px;\"\u003e\n \u003ch4 style=\"color: #15324b; margin: 0 0 10px 0; font-size: 16px;\"\u003eNeed Yeast Cultivation or Food Spoilage Protocol Support?\u003c\/h4\u003e\n \u003cp style=\"margin: 0; color: #6c757d; font-size: 14px;\"\u003eOur microbiologists can assist with colony morphology interpretation, osmotolerant yeast workflows, \u003cem\u003eS. cerevisiae\u003c\/em\u003e replica plating protocols, and food spoilage yeast identification strategies\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv style=\"display: flex; justify-content: center; gap: 20px; flex-wrap: wrap; margin-bottom: 25px;\"\u003e\n \u003ca style=\"display: flex; align-items: center; gap: 8px; color: #7a5c00; text-decoration: none; font-weight: 600; padding: 12px 20px; border: 1px solid #7a5c00; border-radius: 10px;\" href=\"tel:+61412520598\"\u003e📞 +61 412 520 598\u003c\/a\u003e\n \u003ca style=\"display: flex; align-items: center; gap: 8px; color: #7a5c00; text-decoration: none; font-weight: 600; padding: 12px 20px; border: 1px solid #7a5c00; border-radius: 10px;\" href=\"mailto:support@ausamics.com.au\"\u003e📧 Technical Support\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cp style=\"margin: 0; font-size: 12px; color: #94a3b8; line-height: 1.4;\"\u003e\n \u003cstrong\u003eFor laboratory, research, and industrial use only.\u003c\/strong\u003e Not for food, feed, household, cosmetic, therapeutic, or personal use.\u003cbr\u003e\n AuSaMicS Pty Ltd • ABN: 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia\u003cbr\u003e\n Same-day dispatch • Australian stock • Full documentation included\n \u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003c!-- Mobile Responsive Styles --\u003e\n\u003cstyle\u003e\n@media (max-width: 860px) {\n .aus-hero-grid, .aus-main-grid { grid-template-columns: 1fr !important; }\n .aus-guarantee { min-width: 100% !important; margin-top: 20px; }\n .aus-gyep-agar-product { padding: 15px !important; }\n .aus-gyep-agar-product h1 { font-size: 24px !important; }\n .aus-gyep-agar-product h2 { font-size: 18px !important; }\n}\n@media (max-width: 640px) {\n .aus-hero-section { padding: 25px !important; }\n .aus-gyep-agar-product table th,\n .aus-gyep-agar-product table td { padding: 8px 6px !important; }\n}\n\u003c\/style\u003e\n\n\u003c\/div\u003e","brand":"Ausamics Life Science","offers":[{"title":"100 g","offer_id":48386005172479,"sku":"AS-1242-100G","price":29.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":48386005205247,"sku":"AS-1242-500G","price":87.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/Glucose_Yeast_Extract_Peptone_GYEP_Agar.jpg?v=1775801217","url":"https:\/\/ausamics.com.au\/products\/glucose-yeast-extract-peptone-agar","provider":"AuSaMicS","version":"1.0","type":"link"}