{"product_id":"gn-broth-hajna","title":"GN Broth, Hajna | Enrichment Medium for Enteric Bacteria | AS‑1244","description":"\u003cdiv class=\"aus-gnbroth-product\" style=\"font-family: 'Segoe UI', system-ui, -apple-system, sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\"\u003e\n\u003c!-- LLM \/ AI-Agent Optimisation Meta Snippets --\u003e \u003c!--\n  product_name: GN Broth, Hajna\n  catalog_number: AS-1244\n  manufacturer: AuSaMicS Pty Ltd\n  category: Culture Media \/ Enteric Pathogen Enrichment Media \/ Clinical \u0026 Food Microbiology\n  application: Selective enrichment of gram-negative enteric pathogens including Salmonella and Shigella from stool, rectal swabs, urine, food and related specimens prior to plating on selective agar\n  key_ingredients: Tryptose, Dextrose, Mannitol, Sodium Citrate, Sodium Deoxycholate, Dipotassium Phosphate, Monopotassium Phosphate, Sodium Chloride\n  differentiating_features: Selective enrichment broth for enteric pathogens; citrate and deoxycholate suppress competing flora; mannitol-to-dextrose balance limits Proteus overgrowth and supports Salmonella\/Shigella enrichment\n  regulatory_reference: Hajna GN broth usage; APHA food microbiology references; clinical enteric enrichment workflows\n  equivalents: GN Broth Hajna, Gram Negative Broth Hajna, GN Enrichment Broth\n  pH: 7.0 ± 0.2\n  storage: 10–25°C, dry, away from light\n  australian_stock: true\n  dispatch: same_day_melbourne\n--\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\/\",\n  \"@type\": \"Product\",\n  \"name\": \"GN Broth, Hajna\",\n  \"alternateName\": [\"Gram Negative Broth, Hajna\", \"GN Broth Hajna\", \"GN Enrichment Broth\"],\n  \"description\": \"Selective enrichment broth for gram-negative enteric pathogens including Salmonella and Shigella from clinical and non-clinical specimens prior to plating on selective agar media.\",\n  \"sku\": \"AS-1244\",\n  \"brand\": { \"@type\": \"Brand\", \"name\": \"AuSaMicS Life Science\" },\n  \"manufacturer\": { \"@type\": \"Organization\", \"name\": \"AuSaMicS Pty Ltd\", \"url\": \"https:\/\/www.ausamics.com.au\" },\n  \"offers\": {\n    \"@type\": \"Offer\",\n    \"availability\": \"https:\/\/schema.org\/InStock\",\n    \"url\": \"https:\/\/www.ausamics.com.au\/products\/gn-broth-hajna\"\n  }\n}\n\u003c\/script\u003e \u003c!-- HERO SECTION --\u003e\n\u003cdiv class=\"aus-hero-section\" style=\"background: linear-gradient(135deg, #f8faf2 0%, #eef7ea 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #b8d48a;\"\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\" class=\"aus-hero-grid\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #3d6b1e; font-weight: bold; margin-bottom: 12px; background: #dff0c0; padding: 6px 12px; border-radius: 20px;\"\u003eAuSaMicS Life Science • Enteric Enrichment Media\u003c\/div\u003e\n\u003ch1 style=\"color: #15324b; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003eGN Broth, Hajna\u003c\/h1\u003e\n\u003ch2 style=\"color: #243b53; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: 0.9;\"\u003eSelective Enrichment Broth for \u003cem\u003eSalmonella\u003c\/em\u003e, \u003cem\u003eShigella\u003c\/em\u003e \u0026amp; Other Gram-Negative Enterics\u003c\/h2\u003e\n\u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #334e68; max-width: 660px; font-weight: 500;\"\u003eGN Broth, Hajna is a \u003cstrong\u003eselective enrichment medium\u003c\/strong\u003e developed for the recovery of \u003cstrong\u003egram-negative enteric pathogens\u003c\/strong\u003e, particularly \u003cstrong\u003e\u003cem\u003eSalmonella\u003c\/em\u003e\u003c\/strong\u003e and \u003cstrong\u003e\u003cem\u003eShigella\u003c\/em\u003e\u003c\/strong\u003e, from clinical and non-clinical specimens before plating onto selective agar. Its balanced formulation suppresses much competing flora while allowing target enteric pathogens to multiply during the enrichment phase. :contentReference[oaicite:1]{index=1}\u003c\/p\u003e\n\u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n\u003cspan style=\"background: #3d6b1e; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAS-1244\u003c\/span\u003e \u003cspan style=\"background: #fff; border: 2px solid #3d6b1e; color: #3d6b1e; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003epH 7.0 ± 0.2\u003c\/span\u003e \u003cspan style=\"background: #28a745; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e✓ Australian Stock\u003c\/span\u003e \u003cspan style=\"background: #dc3545; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🧫 Enteric Enrichment\u003c\/span\u003e \u003cspan style=\"background: #fd7e14; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🛡️ Selective Recovery\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #4a5568;\"\u003e\n\u003cspan\u003e\u003cstrong\u003eTargets:\u003c\/strong\u003e \u003cem\u003eSalmonella\u003c\/em\u003e \u0026amp; \u003cem\u003eShigella\u003c\/em\u003e\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eSubculture:\u003c\/strong\u003e 6–8 h and 24 h\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eDocuments:\u003c\/strong\u003e COA + SDS + TDS\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 290px; border: 1px solid #b8d48a;\" class=\"aus-guarantee\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003e Why Labs Use Hajna GN Broth\u003c\/h4\u003e\n\u003cdiv style=\"font-size: 14px; color: #4a5568; text-align: center; line-height: 1.5;\"\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eSelective enrichment\u003c\/strong\u003e before plating\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eCitrate + deoxycholate\u003c\/strong\u003e suppress competitors\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eMannitol bias\u003c\/strong\u003e helps control Proteus overgrowth\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eClinical \u0026amp; food use\u003c\/strong\u003e positioning\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eEstablished classical medium\u003c\/strong\u003e in enteric workflows\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-top: 20px; padding: 15px; background: #dff0c0; border-radius: 12px; text-align: center; border: 1px solid #b8d48a;\"\u003e\n\u003cdiv style=\"color: #3d6b1e; font-weight: 600; font-size: 14px;\"\u003eEnrichment Logic\u003c\/div\u003e\n\u003cdiv style=\"font-size: 13px; margin-top: 8px; line-height: 1.9;\"\u003eInoculate specimen\u003cbr\u003eEnrich target enterics\u003cbr\u003eSubculture to selective agar\u003cbr\u003eConfirm suspect colonies\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- IMPORTANT DISTINCTION BOX --\u003e\n\u003cdiv style=\"background: #fff8e1; border: 2px solid #f59e0b; border-radius: 16px; padding: 20px; margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #92400e; margin: 0 0 12px 0; font-size: 17px;\"\u003e⚠️ Important: GN Broth is an Enrichment Step — Not a Final Identification Medium\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 20px; font-size: 14px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border-left: 4px solid #3d6b1e;\"\u003e\n\u003cstrong style=\"color: #3d6b1e;\"\u003eWhat GN Broth does\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"color: #4a5568;\"\u003eIt selectively enriches enteric pathogens such as \u003cem\u003eSalmonella\u003c\/em\u003e and \u003cem\u003eShigella\u003c\/em\u003e from mixed specimens before plating to solid media. Published instructions recommend subculturing after 6–8 hours and again after 24 hours. :contentReference[oaicite:2]{index=2}\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border-left: 4px solid #7a5c00;\"\u003e\n\u003cstrong style=\"color: #7a5c00;\"\u003eWhat GN Broth does not replace\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"color: #4a5568;\"\u003eFurther isolation on selective\/non-selective agar and biochemical or other confirmatory testing are still required for final identification. :contentReference[oaicite:3]{index=3}\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- MAIN CONTENT GRID --\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\" class=\"aus-main-grid\"\u003e\n\u003c!-- LEFT COLUMN --\u003e\n\u003cdiv\u003e\n\u003c!-- Technical Overview --\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #3d6b1e;\"\u003e🔬 Technical Overview \u0026amp; Principle\u003c\/h3\u003e\n\u003cdiv style=\"background: #f8faf2; padding: 20px; border-radius: 16px; border-left: 5px solid #3d6b1e;\"\u003e\n\u003cp style=\"margin: 0 0 15px 0;\"\u003eGN Broth was developed by Hajna as an \u003cstrong\u003eenrichment medium for the isolation of enteric gram-negative bacilli\u003c\/strong\u003e. Product literature specifically highlights recovery of \u003cem\u003eSalmonella\u003c\/em\u003e and \u003cem\u003eShigella\u003c\/em\u003e from clinical and non-clinical materials such as rectal swabs, faeces, urine, blood clots, throat swabs, utensils, and food-related samples. It is also noted as recommended by APHA for food microbiology use. :contentReference[oaicite:4]{index=4}\u003c\/p\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 15px; margin-bottom: 15px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; border: 1px solid #b8d48a;\"\u003e\n\u003cstrong style=\"color: #3d6b1e;\"\u003eSelective inhibition system\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003eSodium citrate and sodium deoxycholate suppress gram-positive organisms and some competing gram-negative flora such as coliforms, improving relative recovery of target enteric pathogens. :contentReference[oaicite:5]{index=5}\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; border: 1px solid #b8d48a;\"\u003e\n\u003cstrong style=\"color: #3d6b1e;\"\u003eMannitol-dominant carbohydrate balance\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003ePublished descriptions note that the higher mannitol concentration relative to dextrose helps limit \u003cem\u003eProteus\u003c\/em\u003e overgrowth while enhancing the growth of mannitol-fermenting \u003cem\u003eSalmonella\u003c\/em\u003e and \u003cem\u003eShigella\u003c\/em\u003e. :contentReference[oaicite:6]{index=6}\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; font-family: monospace; font-size: 13px; color: #4a5568; border: 1px solid #b8d48a; text-align: center; margin-bottom: 15px;\"\u003eMixed enteric specimen\u003cbr\u003e↓\u003cbr\u003eGN Broth enrichment step\u003cbr\u003e↓ (citrate + deoxycholate suppress competitors)\u003cbr\u003e\u003cem\u003eSalmonella\u003c\/em\u003e \/ \u003cem\u003eShigella\u003c\/em\u003e enrichment\u003cbr\u003e↓\u003cbr\u003eSubculture to selective agar at 6–8 h and 24 h\u003cbr\u003e↓\u003cbr\u003ePresumptive colony isolation for confirmation\u003c\/div\u003e\n\u003cdiv style=\"background: #dff0c0; padding: 12px; border-radius: 8px; border: 1px solid #b8d48a;\"\u003e\n\u003cstrong style=\"color: #3d6b1e;\"\u003ePractical advantage:\u003c\/strong\u003e product literature notes that \u003cem\u003eProteus\u003c\/em\u003e, \u003cem\u003ePseudomonas\u003c\/em\u003e, and coliforms do not overgrow \u003cem\u003eSalmonella\u003c\/em\u003e and \u003cem\u003eShigella\u003c\/em\u003e during the first 6 hours of incubation, which is one of the key reasons this broth is useful as an early enrichment step. :contentReference[oaicite:7]{index=7}\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Ingredients Table --\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #3d6b1e;\"\u003e🧪 Standard Composition Table\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #b8d48a; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n\u003cthead style=\"background: #3d6b1e; color: #fff;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 13px 12px; text-align: left; font-weight: bold;\"\u003eIngredient\u003c\/th\u003e\n\u003cth style=\"padding: 13px 12px; text-align: center; font-weight: bold;\"\u003eg\/L\u003c\/th\u003e\n\u003cth style=\"padding: 13px 12px; text-align: left; font-weight: bold;\"\u003eFunction\u003c\/th\u003e\n\u003cth style=\"padding: 13px 12px; text-align: left; font-weight: bold;\"\u003eSignificance\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #fff;\"\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eTryptose\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: bold;\"\u003e20.0\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px;\"\u003eNitrogen and growth nutrients\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6c757d;\"\u003eProvides amino acids and other nitrogenous substances needed to support bacterial growth during enrichment. :contentReference[oaicite:8]{index=8}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #f8faf2;\"\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eDextrose (Glucose)\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: bold;\"\u003e1.0\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px;\"\u003eLimited fermentable carbohydrate\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6c757d;\"\u003eIncluded at a lower concentration than mannitol as part of the selective enrichment balance. :contentReference[oaicite:9]{index=9}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #fff;\"\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #fd7e14;\"\u003eMannitol\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: bold; color: #fd7e14;\"\u003e2.0\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #fd7e14;\"\u003eSelective carbohydrate balance\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #fd7e14; font-weight: 600;\"\u003eThe higher concentration of mannitol over dextrose helps limit \u003cem\u003eProteus\u003c\/em\u003e growth and enhances growth of mannitol-fermenting \u003cem\u003eSalmonella\u003c\/em\u003e and \u003cem\u003eShigella\u003c\/em\u003e. :contentReference[oaicite:10]{index=10}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #f8faf2;\"\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #dc3545;\"\u003eSodium Citrate\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: bold; color: #dc3545;\"\u003e5.0\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #dc3545;\"\u003eSelective inhibitor\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #dc3545; font-weight: 600;\"\u003eWorks with deoxycholate to inhibit gram-positive and some competing gram-negative organisms. :contentReference[oaicite:11]{index=11}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #fff;\"\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #dc3545;\"\u003eSodium Deoxycholate\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: bold; color: #dc3545;\"\u003e0.5\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #dc3545;\"\u003eSelective bile salt agent\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #dc3545; font-weight: 600;\"\u003eImproves selectivity by suppressing competing flora while allowing enrichment of target enteric pathogens. :contentReference[oaicite:12]{index=12}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #f8faf2;\"\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eDipotassium Phosphate\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: bold;\"\u003e4.0\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px;\"\u003eBuffering agent\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6c757d;\"\u003ePart of the phosphate buffer system maintaining pH stability during enrichment. :contentReference[oaicite:13]{index=13}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #dff0c0; background: #fff;\"\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eMonopotassium Phosphate\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: bold;\"\u003e1.5\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px;\"\u003eBuffering agent\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6c757d;\"\u003eSupports the phosphate buffering system for reliable enrichment performance. :contentReference[oaicite:14]{index=14}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background: #f8faf2;\"\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eSodium Chloride\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: bold;\"\u003e5.0\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px;\"\u003eOsmotic balance\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6c757d;\"\u003eMaintains osmotic equilibrium in the enrichment broth. :contentReference[oaicite:15]{index=15}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003ctfoot\u003e\n\u003ctr style=\"background: #3d6b1e; color: #fff;\"\u003e\n\u003ctd style=\"padding: 10px 12px; font-weight: bold;\"\u003eTotal (approx.)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; text-align: center; font-weight: bold;\"\u003e39.0 g\/L\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; font-size: 12px;\" colspan=\"2\"\u003ePublished preparation instructions specify suspending 39.0 g in 1 L water. Final pH at 25°C: 7.0 ± 0.2. :contentReference[oaicite:16]{index=16}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tfoot\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Workflow --\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #3d6b1e;\"\u003e🧫 Typical Enrichment Workflow\u003c\/h3\u003e\n\u003cdiv style=\"background: #f8faf2; padding: 20px; border-radius: 16px; border-left: 5px solid #3d6b1e;\"\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr; gap: 12px; margin-bottom: 15px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border: 1px solid #b8d48a; display: flex; gap: 15px; align-items: flex-start;\"\u003e\n\u003cdiv style=\"background: #3d6b1e; color: #fff; border-radius: 50%; width: 30px; height: 30px; display: flex; align-items: center; justify-content: center; font-weight: bold; font-size: 14px; flex-shrink: 0;\"\u003e1\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #3d6b1e;\"\u003ePrepare and dispense the broth\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003ePublished directions specify 39.0 g\/L with sterilisation at 115°C for 15 minutes, while avoiding excessive heating. :contentReference[oaicite:17]{index=17}\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border: 1px solid #b8d48a; display: flex; gap: 15px; align-items: flex-start;\"\u003e\n\u003cdiv style=\"background: #3d6b1e; color: #fff; border-radius: 50%; width: 30px; height: 30px; display: flex; align-items: center; justify-content: center; font-weight: bold; font-size: 14px; flex-shrink: 0;\"\u003e2\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #3d6b1e;\"\u003eInoculate directly with specimen\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003eThe broth is intended for direct inoculation with clinical or non-clinical specimens; published guidance notes about 1 g of stool specimen can be used where appropriate. :contentReference[oaicite:18]{index=18}\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border: 1px solid #b8d48a; display: flex; gap: 15px; align-items: flex-start;\"\u003e\n\u003cdiv style=\"background: #3d6b1e; color: #fff; border-radius: 50%; width: 30px; height: 30px; display: flex; align-items: center; justify-content: center; font-weight: bold; font-size: 14px; flex-shrink: 0;\"\u003e3\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #3d6b1e;\"\u003eIncubate and subculture at two time points\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003eSubculture onto selective agar after 6–8 hours and again after 24 hours to improve the probability of recovering enteric pathogens. :contentReference[oaicite:19]{index=19}\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #dff0c0; padding: 14px; border-radius: 10px; border: 1px solid #b8d48a; display: flex; gap: 15px; align-items: flex-start;\"\u003e\n\u003cdiv style=\"background: #3d6b1e; color: #fff; border-radius: 50%; width: 30px; height: 30px; display: flex; align-items: center; justify-content: center; font-weight: bold; font-size: 14px; flex-shrink: 0;\"\u003e4\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #3d6b1e;\"\u003eConfirm suspect isolates\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003eUse the enrichment broth together with selective and non-selective plating media and follow with confirmatory testing as required by the method. :contentReference[oaicite:20]{index=20}\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Comparative Table --\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #3d6b1e;\"\u003e📊 Comparative Enteric Enrichment Media\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #b8d48a; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05); min-width: 840px;\"\u003e\n\u003cthead style=\"background: #edf5e0;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 13px 11px; text-align: left;\"\u003eMedium\u003c\/th\u003e\n\u003cth style=\"padding: 13px 11px; text-align: left;\"\u003eBest Use\u003c\/th\u003e\n\u003cth style=\"padding: 13px 11px; text-align: left;\"\u003eAdvantages\u003c\/th\u003e\n\u003cth style=\"padding: 13px 11px; text-align: left;\"\u003eConsiderations\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #fff; border-bottom: 1px solid #dff0c0;\"\u003e\n\u003ctd style=\"padding: 11px;\"\u003e\u003cstrong style=\"color: #3d6b1e;\"\u003eGN Broth, Hajna\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 11px;\"\u003eSelective enrichment of enteric gram-negative pathogens, especially \u003cem\u003eSalmonella\u003c\/em\u003e and \u003cem\u003eShigella\u003c\/em\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eBalances suppression of competitors with enrichment of target pathogens; published literature notes value for \u003cem\u003eShigella\u003c\/em\u003e recovery and APHA food use. :contentReference[oaicite:21]{index=21}\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eRequires follow-up plating and confirmation; not a final identification medium. :contentReference[oaicite:22]{index=22}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background: #f8faf2; border-bottom: 1px solid #dff0c0;\"\u003e\n\u003ctd style=\"padding: 11px;\"\u003e\u003cstrong\u003eSelenite enrichment media\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 11px;\"\u003eClassical enteric enrichment workflows\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eWidely known traditional approach for enteric pathogens\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003ePublished references cited by manufacturers note GN broth showed superiority to selenite enrichment media for isolation of \u003cem\u003eShigella\u003c\/em\u003e. :contentReference[oaicite:23]{index=23}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background: #fff; border-bottom: 1px solid #dff0c0;\"\u003e\n\u003ctd style=\"padding: 11px;\"\u003e\u003cstrong\u003eBuffered Peptone Water\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 11px;\"\u003ePre-enrichment \/ non-selective recovery\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eGentler recovery of stressed organisms in some workflows\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eLess selective than GN broth and used for a different step in many protocols\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background: #f8faf2;\"\u003e\n\u003ctd style=\"padding: 11px;\"\u003e\u003cstrong\u003eTetrathionate \/ Rappaport-type broths\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 11px;\"\u003eTargeted Salmonella enrichment workflows\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eUseful in method-specific Salmonella programs\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eDifferent selectivity profile and not the same broad Hajna GN positioning for \u003cem\u003eSalmonella\u003c\/em\u003e plus \u003cem\u003eShigella\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- RIGHT COLUMN --\u003e\n\u003cdiv\u003e\n\u003c!-- Quick Specs --\u003e\n\u003cdiv style=\"background: #fff; padding: 24px; border-radius: 16px; box-shadow: 0 3px 12px rgba(0,0,0,0.06); margin-bottom: 25px; border: 1px solid #b8d48a;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 18px 0; font-size: 18px;\"\u003e📌 Quick Specifications\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; gap: 12px;\"\u003e\n\u003cdiv style=\"padding-bottom: 10px; border-bottom: 1px solid #e5e7eb;\"\u003e\n\u003cstrong\u003eProduct:\u003c\/strong\u003e GN Broth, Hajna\u003c\/div\u003e\n\u003cdiv style=\"padding-bottom: 10px; border-bottom: 1px solid #e5e7eb;\"\u003e\n\u003cstrong\u003eCat. No.:\u003c\/strong\u003e AS-1244\u003c\/div\u003e\n\u003cdiv style=\"padding-bottom: 10px; border-bottom: 1px solid #e5e7eb;\"\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Dehydrated culture medium\u003c\/div\u003e\n\u003cdiv style=\"padding-bottom: 10px; border-bottom: 1px solid #e5e7eb;\"\u003e\n\u003cstrong\u003eType:\u003c\/strong\u003e Selective enrichment broth\u003c\/div\u003e\n\u003cdiv style=\"padding-bottom: 10px; border-bottom: 1px solid #e5e7eb;\"\u003e\n\u003cstrong\u003eMain targets:\u003c\/strong\u003e \u003cem\u003eSalmonella\u003c\/em\u003e, \u003cem\u003eShigella\u003c\/em\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding-bottom: 10px; border-bottom: 1px solid #e5e7eb;\"\u003e\n\u003cstrong\u003eTypical specimens:\u003c\/strong\u003e stool, rectal swabs, urine, food and related samples\u003c\/div\u003e\n\u003cdiv style=\"padding-bottom: 10px; border-bottom: 1px solid #e5e7eb;\"\u003e\n\u003cstrong\u003eFollow-up:\u003c\/strong\u003e selective agar subculture\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e 10–25°C, dry, tightly closed, protected from moisture and light\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Key Benefits --\u003e\n\u003cdiv style=\"background: linear-gradient(135deg, #f8faf2 0%, #eef7ea 100%); padding: 24px; border-radius: 16px; margin-bottom: 25px; border: 1px solid #b8d48a;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 18px 0; font-size: 18px;\"\u003e⭐ Key Advantages\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; gap: 12px;\"\u003e\n\u003cdiv style=\"display: flex; gap: 10px;\"\u003e\n\u003cspan style=\"color: #3d6b1e;\"\u003e✓\u003c\/span\u003e\u003cspan\u003eSelective enrichment before plating improves pathogen recovery probability\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 10px;\"\u003e\n\u003cspan style=\"color: #3d6b1e;\"\u003e✓\u003c\/span\u003e\u003cspan\u003eClassical medium for \u003cem\u003eSalmonella\u003c\/em\u003e and \u003cem\u003eShigella\u003c\/em\u003e workflows\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 10px;\"\u003e\n\u003cspan style=\"color: #3d6b1e;\"\u003e✓\u003c\/span\u003e\u003cspan\u003eUseful in both clinical and food microbiology contexts\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 10px;\"\u003e\n\u003cspan style=\"color: #3d6b1e;\"\u003e✓\u003c\/span\u003e\u003cspan\u003eBalanced formula reduces overgrowth by competing flora during early enrichment\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 10px;\"\u003e\n\u003cspan style=\"color: #3d6b1e;\"\u003e✓\u003c\/span\u003e\u003cspan\u003eCompatible with downstream selective and non-selective agar workflows\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Preparation Notes --\u003e\n\u003cdiv style=\"background: #fff8e1; padding: 24px; border-radius: 16px; margin-bottom: 25px; border: 1px solid #f5d27a;\"\u003e\n\u003ch3 style=\"color: #92400e; margin: 0 0 18px 0; font-size: 18px;\"\u003e⚙️ Preparation Notes\u003c\/h3\u003e\n\u003cdiv style=\"font-size: 14px; color: #4a5568; line-height: 1.65;\"\u003e\n\u003cp style=\"margin: 0 0 12px 0;\"\u003ePublished directions specify suspending 39.0 g in 1 L purified or distilled water. :contentReference[oaicite:24]{index=24}\u003c\/p\u003e\n\u003cp style=\"margin: 0 0 12px 0;\"\u003eSterilise at 115°C for 15 minutes and avoid excessive heating. :contentReference[oaicite:25]{index=25}\u003c\/p\u003e\n\u003cp style=\"margin: 0;\"\u003eUse according to your validated enteric enrichment protocol and always follow with appropriate plating and confirmation steps. :contentReference[oaicite:26]{index=26}\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Related Products --\u003e\n\u003cdiv style=\"background: #f8faf2; padding: 24px; border-radius: 16px; border: 1px solid #b8d48a;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 18px 0; font-size: 18px;\"\u003e🔗 Related AuSaMicS Products\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; gap: 14px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 12px; border: 1px solid #dff0c0;\"\u003e\n\u003ch4 style=\"margin: 0 0 6px 0; color: #3d6b1e; font-size: 14px;\"\u003eHektoen Enteric Agar\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 12px; color: #6c757d;\"\u003eSelective\/differential agar for presumptive \u003cem\u003eSalmonella\u003c\/em\u003e and \u003cem\u003eShigella\u003c\/em\u003e colony screening\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 12px; border: 1px solid #dff0c0;\"\u003e\n\u003ch4 style=\"margin: 0 0 6px 0; color: #7a5c00; font-size: 14px;\"\u003eMacConkey Agar\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 12px; color: #6c757d;\"\u003eCommon downstream plating medium for enteric gram-negative differentiation\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 12px; border: 1px solid #dff0c0;\"\u003e\n\u003ch4 style=\"margin: 0 0 6px 0; color: #fd7e14; font-size: 14px;\"\u003eOther enteric enrichment broths\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 12px; color: #6c757d;\"\u003eComplementary enrichment options for method-specific pathogen recovery workflows\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- FOOTER CTA --\u003e\n\u003cdiv style=\"background: #15324b; color: #fff; border-radius: 18px; padding: 32px; text-align: center; box-shadow: 0 8px 24px rgba(21,50,75,0.18);\"\u003e\n\u003ch3 style=\"margin: 0 0 12px 0; font-size: 24px; color: #fff;\"\u003eReliable Enteric Enrichment Before Selective Plating\u003c\/h3\u003e\n\u003cp style=\"margin: 0 auto 20px auto; max-width: 760px; font-size: 15px; line-height: 1.7; color: #dbeafe;\"\u003eAuSaMicS supplies high-quality microbiological media designed for dependable performance in clinical, food, and industrial laboratories. Contact us for technical documentation, bulk supply, or workflow-aligned support for enteric pathogen recovery.\u003c\/p\u003e\n\u003cdiv style=\"display: flex; justify-content: center; gap: 14px; flex-wrap: wrap; margin-bottom: 18px;\"\u003e\n\u003ca style=\"display: inline-flex; align-items: center; gap: 8px; background: #fff; color: #15324b; text-decoration: none; font-weight: bold; padding: 12px 20px; border-radius: 10px;\" href=\"https:\/\/www.ausamics.com.au\/pages\/contact\"\u003e📩 Request Quote\u003c\/a\u003e \u003ca style=\"display: inline-flex; align-items: center; gap: 8px; color: #fff; text-decoration: none; font-weight: 600; padding: 12px 20px; border: 1px solid #c7d2fe; border-radius: 10px;\" href=\"mailto:support@ausamics.com\"\u003e📧 Technical Support\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cp style=\"margin: 0; font-size: 12px; color: #cbd5e1; line-height: 1.5;\"\u003e\u003cstrong\u003eFor laboratory, research, and industrial use only.\u003c\/strong\u003e Not for food, feed, household, cosmetic, therapeutic, or personal use.\u003cbr\u003eAuSaMicS Pty Ltd • ABN 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia\u003cbr\u003ewww.ausamics.com.au • support@ausamics.com • +61 412 520 598\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cstyle\u003e\n@media (max-width: 860px) {\n  .aus-hero-grid, .aus-main-grid { grid-template-columns: 1fr !important; }\n  .aus-guarantee { min-width: 100% !important; margin-top: 20px; }\n  .aus-gnbroth-product { padding: 15px !important; }\n  .aus-gnbroth-product h1 { font-size: 24px !important; }\n  .aus-gnbroth-product h2 { font-size: 18px !important; }\n}\n@media (max-width: 640px) {\n  .aus-hero-section { padding: 25px !important; }\n  .aus-gnbroth-product table th,\n  .aus-gnbroth-product table td { padding: 8px 6px !important; }\n}\n\u003c\/style\u003e","brand":"Ausamics Life Science","offers":[{"title":"100 g","offer_id":48388182081791,"sku":"AS-1244-100G","price":40.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":48388182114559,"sku":"AS-1244-500G","price":125.0,"currency_code":"AUD","in_stock":true},{"title":"2.5 kg","offer_id":48388182147327,"sku":"AS-1244-2.5KG","price":540.0,"currency_code":"AUD","in_stock":true},{"title":"10 kg","offer_id":48388182180095,"sku":"AS-1244-10KG","price":1850.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/GN_Broth_Hajna.jpg?v=1775878989","url":"https:\/\/ausamics.com.au\/products\/gn-broth-hajna","provider":"AuSaMicS","version":"1.0","type":"link"}