{"product_id":"hanahan-s-broth","title":"Hanahan’s Broth (SOB Medium) | Competent Cell Preparation Medium | AS‑1245","description":"\u003cdiv class=\"aus-sob-product\" style=\"font-family: 'Segoe UI', system-ui, -apple-system, sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\"\u003e\n\u003c!-- LLM \/ AI-Agent Optimisation Meta Snippets --\u003e \u003c!--\n  product_name: Hanahan's Broth: SOB Medium\n  catalog_number: AS-1245\n  manufacturer: AuSaMicS Pty Ltd\n  category: Molecular Biology \/ Cloning Media \/ Competent Cell Media\n  application: Preparation and transformation of competent Escherichia coli; non-selective cultivation of recombinant E. coli for cloning, plasmid propagation, and recombinant protein workflows\n  key_ingredients: Tryptone, Yeast Extract, Sodium Chloride, Potassium Chloride, Magnesium Sulphate\n  differentiating_features: Richer than LB in peptone\/amino acids\/peptides; standard Hanahan SOB formulation for competent cell preparation; low-salt cloning medium; precursor to SOC\n  regulatory_reference: Standard molecular biology laboratory practice; Hanahan competent-cell workflows\n  equivalents: Hanahan's Broth, SOB Medium, Super Optimal Broth\n  pH: 7.0 ± 0.2\n  storage: 10–25°C, dry, away from light\n  australian_stock: true\n  dispatch: same_day_melbourne\n--\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n  \"@context\": \"https:\/\/schema.org\/\",\n  \"@type\": \"Product\",\n  \"name\": \"Hanahan's Broth: SOB Medium\",\n  \"alternateName\": [\"SOB Medium\", \"Hanahan's Broth\", \"Super Optimal Broth\"],\n  \"description\": \"A rich molecular biology broth used for preparation and transformation of competent E. coli and for non-selective cultivation of recombinant strains in cloning and plasmid workflows.\",\n  \"sku\": \"AS-1245\",\n  \"brand\": { \"@type\": \"Brand\", \"name\": \"AuSaMicS Life Science\" },\n  \"manufacturer\": { \"@type\": \"Organization\", \"name\": \"AuSaMicS Pty Ltd\", \"url\": \"https:\/\/www.ausamics.com.au\" },\n  \"offers\": {\n    \"@type\": \"Offer\",\n    \"availability\": \"https:\/\/schema.org\/InStock\",\n    \"url\": \"https:\/\/www.ausamics.com.au\/products\/hanahans-broth-sob-medium\"\n  }\n}\n\u003c\/script\u003e \u003c!-- HERO SECTION --\u003e\n\u003cdiv class=\"aus-hero-section\" style=\"background: linear-gradient(135deg, #f7fafc 0%, #eef4ff 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #c9d8f0;\"\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\" class=\"aus-hero-grid\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #234a84; font-weight: bold; margin-bottom: 12px; background: #dde9fb; padding: 6px 12px; border-radius: 20px;\"\u003eAuSaMicS Life Science • Molecular Biology Media\u003c\/div\u003e\n\u003ch1 style=\"color: #15324b; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003eHanahan’s Broth: SOB Medium\u003c\/h1\u003e\n\u003ch2 style=\"color: #243b53; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: 0.9;\"\u003eRich Broth for Competent \u003cem\u003eE. coli\u003c\/em\u003e, Cloning \u0026amp; Transformation Workflows\u003c\/h2\u003e\n\u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #334e68; max-width: 660px; font-weight: 500;\"\u003eHanahan’s Broth, also known as \u003cstrong\u003eSOB Medium\u003c\/strong\u003e, is a \u003cstrong\u003erich, non-selective molecular biology broth\u003c\/strong\u003e used for the preparation and transformation of \u003cstrong\u003ecompetent \u003cem\u003eEscherichia coli\u003c\/em\u003e\u003c\/strong\u003e. It is widely used in cloning, plasmid propagation, and recombinant expression workflows where a nutritionally rich, low-salt medium is preferred over standard LB. :contentReference[oaicite:1]{index=1}\u003c\/p\u003e\n\u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n\u003cspan style=\"background: #234a84; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAS-1245\u003c\/span\u003e \u003cspan style=\"background: #fff; border: 2px solid #234a84; color: #234a84; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003epH 7.0 ± 0.2\u003c\/span\u003e \u003cspan style=\"background: #28a745; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e✓ Australian Stock\u003c\/span\u003e \u003cspan style=\"background: #6f42c1; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🧬 Competent Cell Media\u003c\/span\u003e \u003cspan style=\"background: #fd7e14; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e⚡ Transformation Workflow\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #4a5568;\"\u003e\n\u003cspan\u003e\u003cstrong\u003eUse:\u003c\/strong\u003e Competent \u003cem\u003eE. coli\u003c\/em\u003e preparation\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eApplication:\u003c\/strong\u003e Cloning \u0026amp; plasmid work\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eDocuments:\u003c\/strong\u003e COA + SDS + TDS\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 290px; border: 1px solid #c9d8f0;\" class=\"aus-guarantee\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003e Why Molecular Labs Use SOB\u003c\/h4\u003e\n\u003cdiv style=\"font-size: 14px; color: #4a5568; text-align: center; line-height: 1.5;\"\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eCompetent cell prep\u003c\/strong\u003e support\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eTransformation-ready\u003c\/strong\u003e rich broth\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eRicher than LB\u003c\/strong\u003e in peptides and amino acids\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eNon-selective growth\u003c\/strong\u003e of recombinant \u003cem\u003eE. coli\u003c\/em\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eBase for SOC workflows\u003c\/strong\u003e with glucose addition\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-top: 20px; padding: 15px; background: #dde9fb; border-radius: 12px; text-align: center; border: 1px solid #c9d8f0;\"\u003e\n\u003cdiv style=\"color: #234a84; font-weight: 600; font-size: 14px;\"\u003eCommon Workflow Logic\u003c\/div\u003e\n\u003cdiv style=\"font-size: 13px; margin-top: 8px; line-height: 1.9;\"\u003ePrepare competent cells\u003cbr\u003eTransform with DNA\u003cbr\u003eRecover in rich broth\u003cbr\u003eProceed to selection \/ cloning\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- IMPORTANT DISTINCTION BOX --\u003e\n\u003cdiv style=\"background: #fff8e1; border: 2px solid #f59e0b; border-radius: 16px; padding: 20px; margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #92400e; margin: 0 0 12px 0; font-size: 17px;\"\u003e⚠️ Important: SOB Medium vs SOC Medium\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 20px; font-size: 14px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border-left: 4px solid #234a84;\"\u003e\n\u003cstrong style=\"color: #234a84;\"\u003eHanahan’s Broth \/ SOB Medium (AS-1245)\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"color: #4a5568;\"\u003eA rich cloning broth used for competent cell preparation and recombinant \u003cem\u003eE. coli\u003c\/em\u003e culture. It is richer than LB and does \u003cstrong\u003enot inherently include glucose\u003c\/strong\u003e. :contentReference[oaicite:2]{index=2}\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border-left: 4px solid #856404;\"\u003e\n\u003cstrong style=\"color: #856404;\"\u003eSOC Medium\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"color: #4a5568;\"\u003eSOC is a \u003cstrong\u003emodified SOB medium with added glucose\u003c\/strong\u003e, commonly used in the post-transformation recovery step to improve transformation efficiency in appropriate workflows. :contentReference[oaicite:3]{index=3}\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- MAIN CONTENT GRID --\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\" class=\"aus-main-grid\"\u003e\n\u003c!-- LEFT COLUMN --\u003e\n\u003cdiv\u003e\n\u003c!-- Technical Overview --\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #234a84;\"\u003e🔬 Technical Overview \u0026amp; Principle\u003c\/h3\u003e\n\u003cdiv style=\"background: #f7fafc; padding: 20px; border-radius: 16px; border-left: 5px solid #234a84;\"\u003e\n\u003cp style=\"margin: 0 0 15px 0;\"\u003eHanahan’s Broth (SOB Medium) is a \u003cstrong\u003estandard rich medium for molecular cloning laboratories\u003c\/strong\u003e. Sigma describes it as being used for the preparation and transformation of competent \u003cem\u003eE. coli\u003c\/em\u003e, and for the non-selective cultivation of recombinant strains used in cloning, plasmid DNA production, and recombinant protein workflows. :contentReference[oaicite:4]{index=4}\u003c\/p\u003e\n\u003cp style=\"margin: 0 0 15px 0;\"\u003eCompared with standard LB, SOB contains \u003cstrong\u003ehigher levels of peptone-derived nutrients, amino acids, and peptides\u003c\/strong\u003e, which is one reason it is widely associated with high-efficiency competent-cell methods. Magnesium is also a key component of the system, and supplier notes describe magnesium sulfate as a necessary component aiding DNA replication. :contentReference[oaicite:5]{index=5}\u003c\/p\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; border: 1px solid #c9d8f0; margin-bottom: 15px;\"\u003e\n\u003cstrong style=\"color: #234a84;\"\u003eWhy this medium performs well:\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003e Tryptone and yeast extract provide abundant nitrogen, peptides, amino acids, and vitamins; NaCl and KCl maintain suitable osmotic conditions; and MgSO\u003csub\u003e4\u003c\/sub\u003e supports transformation-associated cell recovery and recombinant-cell handling. This combination makes SOB a practical step up from routine LB in competent-cell and cloning workflows. :contentReference[oaicite:6]{index=6} \u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #dde9fb; padding: 12px; border-radius: 8px; border: 1px solid #c9d8f0;\"\u003e\n\u003cstrong style=\"color: #234a84;\"\u003eTypical applications:\u003c\/strong\u003e competent \u003cem\u003eE. coli\u003c\/em\u003e preparation, non-selective growth after cloning steps, plasmid propagation, and recombinant protein strain cultivation. Sigma also notes reported use with \u003cem\u003eCupriavidus necator\u003c\/em\u003e H16 competent cells. :contentReference[oaicite:7]{index=7}\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Ingredients Table --\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #234a84;\"\u003e🧪 Standard SOB Composition\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #c9d8f0; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n\u003cthead style=\"background: #234a84; color: #fff;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 13px 12px; text-align: left; font-weight: bold;\"\u003eIngredient\u003c\/th\u003e\n\u003cth style=\"padding: 13px 12px; text-align: center; font-weight: bold;\"\u003eTypical g\/L\u003c\/th\u003e\n\u003cth style=\"padding: 13px 12px; text-align: left; font-weight: bold;\"\u003eFunction\u003c\/th\u003e\n\u003cth style=\"padding: 13px 12px; text-align: left; font-weight: bold;\"\u003ePerformance Contribution\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #dde9fb; background: #fff;\"\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eTryptone\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: bold;\"\u003e20.0\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px;\"\u003ePeptides, amino acids, nitrogen source\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6c757d;\"\u003eSupports rapid growth and recovery of recombinant \u003cem\u003eE. coli\u003c\/em\u003e; the richer peptide load is part of what differentiates SOB from LB. :contentReference[oaicite:8]{index=8}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #dde9fb; background: #f7fafc;\"\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eYeast Extract\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: bold;\"\u003e5.0\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px;\"\u003eVitamins, growth factors, organic nutrients\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6c757d;\"\u003eProvides water-soluble vitamins and additional growth factors for competent and recombinant cell workflows. :contentReference[oaicite:9]{index=9}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #dde9fb; background: #fff;\"\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003eSodium Chloride\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: bold;\"\u003e0.5\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px;\"\u003eIsotonic support\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6c757d;\"\u003eContributes to osmotic balance in low-salt competent-cell culture conditions. Standard SOB formulation value.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #dde9fb; background: #f7fafc;\"\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600;\"\u003ePotassium Chloride\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: bold;\"\u003e0.186\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px;\"\u003eOsmotic and ionic balance\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6c757d;\"\u003eSupplier notes describe KCl together with NaCl as maintaining isotonic conditions. :contentReference[oaicite:10]{index=10}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background: #fff;\"\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #6f42c1;\"\u003eMagnesium Sulphate\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; text-align: center; font-weight: bold; color: #6f42c1;\"\u003e2.4\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-weight: 600; color: #6f42c1;\"\u003eMg²⁺ source\u003c\/td\u003e\n\u003ctd style=\"padding: 11px 12px; font-size: 12px; color: #6f42c1; font-weight: 600;\"\u003eSigma describes magnesium sulfate as a necessary component for DNA replication, making it a defining part of the SOB competent-cell workflow. :contentReference[oaicite:11]{index=11}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003ctfoot\u003e\n\u003ctr style=\"background: #234a84; color: #fff;\"\u003e\n\u003ctd style=\"padding: 10px 12px; font-weight: bold;\"\u003eTotal (approx.)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; text-align: center; font-weight: bold;\"\u003e28 g\/L\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 12px; font-size: 12px;\" colspan=\"2\"\u003eSigma preparation note specifies suspending 28 g powder in 1 L water. Final pH for standard SOB is commonly around 7.0. :contentReference[oaicite:12]{index=12}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tfoot\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Workflow --\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #234a84;\"\u003e🧬 Typical Molecular Biology Workflow\u003c\/h3\u003e\n\u003cdiv style=\"background: #f7fafc; padding: 20px; border-radius: 16px; border-left: 5px solid #234a84;\"\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr; gap: 12px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border: 1px solid #c9d8f0; display: flex; gap: 15px; align-items: flex-start;\"\u003e\n\u003cdiv style=\"background: #234a84; color: #fff; border-radius: 50%; width: 30px; height: 30px; display: flex; align-items: center; justify-content: center; font-weight: bold; font-size: 14px; flex-shrink: 0;\"\u003e1\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #234a84;\"\u003ePrepare the medium\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003ePrepare according to the product instructions and validated lab method. Sigma’s powder preparation note is 28 g\/L followed by autoclaving, with additions made after cooling when required. :contentReference[oaicite:13]{index=13}\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border: 1px solid #c9d8f0; display: flex; gap: 15px; align-items: flex-start;\"\u003e\n\u003cdiv style=\"background: #234a84; color: #fff; border-radius: 50%; width: 30px; height: 30px; display: flex; align-items: center; justify-content: center; font-weight: bold; font-size: 14px; flex-shrink: 0;\"\u003e2\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #234a84;\"\u003eUse for competent-cell preparation or recovery\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003eSOB is widely used in competent \u003cem\u003eE. coli\u003c\/em\u003e workflows because its rich nutrient profile supports gentle cell handling and transformation-related recovery. :contentReference[oaicite:14]{index=14}\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 10px; border: 1px solid #c9d8f0; display: flex; gap: 15px; align-items: flex-start;\"\u003e\n\u003cdiv style=\"background: #234a84; color: #fff; border-radius: 50%; width: 30px; height: 30px; display: flex; align-items: center; justify-content: center; font-weight: bold; font-size: 14px; flex-shrink: 0;\"\u003e3\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #234a84;\"\u003eGrow recombinant strains non-selectively\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003eSupplier documentation also positions SOB for non-selective cultivation of recombinant \u003cem\u003eE. coli\u003c\/em\u003e used in cloning, plasmid DNA production, and recombinant protein workflows. :contentReference[oaicite:15]{index=15}\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #dde9fb; padding: 14px; border-radius: 10px; border: 1px solid #c9d8f0; display: flex; gap: 15px; align-items: flex-start;\"\u003e\n\u003cdiv style=\"background: #234a84; color: #fff; border-radius: 50%; width: 30px; height: 30px; display: flex; align-items: center; justify-content: center; font-weight: bold; font-size: 14px; flex-shrink: 0;\"\u003e4\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #234a84;\"\u003eConvert to SOC when needed\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 13px; color: #4a5568;\"\u003eFor some transformation workflows, glucose is added to create SOC, the catabolite-repressed derivative used in post-transformation recovery. :contentReference[oaicite:16]{index=16}\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Comparative Table --\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #234a84;\"\u003e📊 Comparative Cloning \u0026amp; Growth Media\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #c9d8f0; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05); min-width: 840px;\"\u003e\n\u003cthead style=\"background: #eef4ff;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 13px 11px; text-align: left;\"\u003eMedium\u003c\/th\u003e\n\u003cth style=\"padding: 13px 11px; text-align: left;\"\u003eBest Use\u003c\/th\u003e\n\u003cth style=\"padding: 13px 11px; text-align: left;\"\u003eAdvantages\u003c\/th\u003e\n\u003cth style=\"padding: 13px 11px; text-align: left;\"\u003eConsiderations\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #fff; border-bottom: 1px solid #dde9fb;\"\u003e\n\u003ctd style=\"padding: 11px;\"\u003e\u003cstrong style=\"color: #234a84;\"\u003eSOB Medium\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 11px;\"\u003eCompetent-cell preparation, transformation, rich non-selective cloning culture\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eRicher than LB in peptone\/amino acids\/peptides; standard Hanahan workflow medium. :contentReference[oaicite:17]{index=17}\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eNo glucose by default; often paired with downstream selective plating.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background: #f7fafc; border-bottom: 1px solid #dde9fb;\"\u003e\n\u003ctd style=\"padding: 11px;\"\u003e\u003cstrong\u003eSOC Medium\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 11px;\"\u003ePost-transformation recovery\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eGlucose-containing derivative of SOB used to improve transformation recovery in suitable workflows. :contentReference[oaicite:18]{index=18}\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eDistinct medium, not interchangeable with unmodified SOB in every protocol.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background: #fff; border-bottom: 1px solid #dde9fb;\"\u003e\n\u003ctd style=\"padding: 11px;\"\u003e\u003cstrong\u003eLB Broth\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 11px;\"\u003eRoutine \u003cem\u003eE. coli\u003c\/em\u003e growth\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eSimple, widely used, economical standard medium\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eLess enriched than SOB for competent-cell oriented workflows. Inference supported by Sigma’s comparison noting SOB is richer than LB. :contentReference[oaicite:19]{index=19}\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background: #f7fafc;\"\u003e\n\u003ctd style=\"padding: 11px;\"\u003e\u003cstrong\u003eTerrific Broth\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 11px;\"\u003eHigh-density plasmid or expression culture\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eVery rich, strong biomass generation\u003c\/td\u003e\n\u003ctd style=\"padding: 11px; font-size: 12px;\"\u003eUsually positioned more for dense growth than classic competent-cell preparation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- RIGHT COLUMN --\u003e\n\u003cdiv\u003e\n\u003c!-- Quick Specs --\u003e\n\u003cdiv style=\"background: #fff; padding: 24px; border-radius: 16px; box-shadow: 0 3px 12px rgba(0,0,0,0.06); margin-bottom: 25px; border: 1px solid #c9d8f0;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 18px 0; font-size: 18px;\"\u003e📌 Quick Specifications\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; gap: 12px;\"\u003e\n\u003cdiv style=\"padding-bottom: 10px; border-bottom: 1px solid #e5e7eb;\"\u003e\n\u003cstrong\u003eProduct:\u003c\/strong\u003e Hanahan’s Broth: SOB Medium\u003c\/div\u003e\n\u003cdiv style=\"padding-bottom: 10px; border-bottom: 1px solid #e5e7eb;\"\u003e\n\u003cstrong\u003eCat. No.:\u003c\/strong\u003e AS-1245\u003c\/div\u003e\n\u003cdiv style=\"padding-bottom: 10px; border-bottom: 1px solid #e5e7eb;\"\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Dehydrated culture medium\u003c\/div\u003e\n\u003cdiv style=\"padding-bottom: 10px; border-bottom: 1px solid #e5e7eb;\"\u003e\n\u003cstrong\u003eType:\u003c\/strong\u003e Rich, non-selective molecular biology broth\u003c\/div\u003e\n\u003cdiv style=\"padding-bottom: 10px; border-bottom: 1px solid #e5e7eb;\"\u003e\n\u003cstrong\u003eMain organism:\u003c\/strong\u003e \u003cem\u003eE. coli\u003c\/em\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding-bottom: 10px; border-bottom: 1px solid #e5e7eb;\"\u003e\n\u003cstrong\u003eMain use:\u003c\/strong\u003e Competent-cell preparation and transformation\u003c\/div\u003e\n\u003cdiv style=\"padding-bottom: 10px; border-bottom: 1px solid #e5e7eb;\"\u003e\n\u003cstrong\u003eRelated medium:\u003c\/strong\u003e SOC after glucose addition\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e 10–25°C, dry, tightly closed, protected from moisture and light\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Key Benefits --\u003e\n\u003cdiv style=\"background: linear-gradient(135deg, #f7fafc 0%, #eef4ff 100%); padding: 24px; border-radius: 16px; margin-bottom: 25px; border: 1px solid #c9d8f0;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 18px 0; font-size: 18px;\"\u003e⭐ Key Advantages\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; gap: 12px;\"\u003e\n\u003cdiv style=\"display: flex; gap: 10px;\"\u003e\n\u003cspan style=\"color: #234a84;\"\u003e✓\u003c\/span\u003e\u003cspan\u003eDesigned for competent-cell and transformation workflows\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 10px;\"\u003e\n\u003cspan style=\"color: #234a84;\"\u003e✓\u003c\/span\u003e\u003cspan\u003eRicher than LB for recovery-sensitive cloning steps\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 10px;\"\u003e\n\u003cspan style=\"color: #234a84;\"\u003e✓\u003c\/span\u003e\u003cspan\u003eSupports recombinant \u003cem\u003eE. coli\u003c\/em\u003e growth without selection pressure\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 10px;\"\u003e\n\u003cspan style=\"color: #234a84;\"\u003e✓\u003c\/span\u003e\u003cspan\u003eStandard molecular biology formulation familiar to many labs\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 10px;\"\u003e\n\u003cspan style=\"color: #234a84;\"\u003e✓\u003c\/span\u003e\u003cspan\u003eCan be extended into SOC-based workflows when needed\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Preparation Notes --\u003e\n\u003cdiv style=\"background: #fff8e1; padding: 24px; border-radius: 16px; margin-bottom: 25px; border: 1px solid #f5d27a;\"\u003e\n\u003ch3 style=\"color: #92400e; margin: 0 0 18px 0; font-size: 18px;\"\u003e⚙️ Preparation Notes\u003c\/h3\u003e\n\u003cdiv style=\"font-size: 14px; color: #4a5568; line-height: 1.65;\"\u003e\n\u003cp style=\"margin: 0 0 12px 0;\"\u003ePrepare according to the product instructions and your validated laboratory method.\u003c\/p\u003e\n\u003cp style=\"margin: 0 0 12px 0;\"\u003eSupplier guidance indicates autoclaving the powder suspension, then allowing the medium to cool before post-sterilisation additions where required. :contentReference[oaicite:20]{index=20}\u003c\/p\u003e\n\u003cp style=\"margin: 0;\"\u003eFor transformation workflows, use only with validated competent-cell and recovery protocols appropriate to your strain and vector system.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Related Products --\u003e\n\u003cdiv style=\"background: #f7fafc; padding: 24px; border-radius: 16px; border: 1px solid #c9d8f0;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 18px 0; font-size: 18px;\"\u003e🔗 Related AuSaMicS Products\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; gap: 14px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 12px; border: 1px solid #dde9fb;\"\u003e\n\u003ch4 style=\"margin: 0 0 6px 0; color: #234a84; font-size: 14px;\"\u003eSOC Medium\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 12px; color: #6c757d;\"\u003eGlucose-containing derivative used for post-transformation recovery workflows\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 12px; border: 1px solid #dde9fb;\"\u003e\n\u003ch4 style=\"margin: 0 0 6px 0; color: #856404; font-size: 14px;\"\u003eLB Broth \/ LB Agar\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 12px; color: #6c757d;\"\u003eRoutine cloning and maintenance media for standard \u003cem\u003eE. coli\u003c\/em\u003e work\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 12px; border: 1px solid #dde9fb;\"\u003e\n\u003ch4 style=\"margin: 0 0 6px 0; color: #6f42c1; font-size: 14px;\"\u003eTerrific Broth\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 12px; color: #6c757d;\"\u003eVery rich broth for high-density plasmid and expression cultures\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- FOOTER CTA --\u003e\n\u003cdiv style=\"background: #15324b; color: #fff; border-radius: 18px; padding: 32px; text-align: center; box-shadow: 0 8px 24px rgba(21,50,75,0.18);\"\u003e\n\u003ch3 style=\"margin: 0 0 12px 0; font-size: 24px; color: #fff;\"\u003eReliable Competent-Cell Media for Molecular Biology Workflows\u003c\/h3\u003e\n\u003cp style=\"margin: 0 auto 20px auto; max-width: 760px; font-size: 15px; line-height: 1.7; color: #dbeafe;\"\u003eAuSaMicS supplies high-quality microbiological and molecular biology media designed for dependable laboratory performance. Contact us for technical documentation, bulk supply, or support aligned to competent-cell and cloning workflows.\u003c\/p\u003e\n\u003cdiv style=\"display: flex; justify-content: center; gap: 14px; flex-wrap: wrap; margin-bottom: 18px;\"\u003e\n\u003ca style=\"display: inline-flex; align-items: center; gap: 8px; background: #fff; color: #15324b; text-decoration: none; font-weight: bold; padding: 12px 20px; border-radius: 10px;\" href=\"https:\/\/www.ausamics.com.au\/pages\/contact\"\u003e📩 Request Quote\u003c\/a\u003e \u003ca style=\"display: inline-flex; align-items: center; gap: 8px; color: #fff; text-decoration: none; font-weight: 600; padding: 12px 20px; border: 1px solid #c7d2fe; border-radius: 10px;\" href=\"mailto:support@ausamics.com\"\u003e📧 Technical Support\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cp style=\"margin: 0; font-size: 12px; color: #cbd5e1; line-height: 1.5;\"\u003e\u003cstrong\u003eFor laboratory, research, and industrial use only.\u003c\/strong\u003e Not for food, feed, household, cosmetic, therapeutic, or personal use.\u003cbr\u003eAuSaMicS Pty Ltd • ABN 56 676 640 467 • 31 Longview CT, Thomastown, VIC 3074, Australia\u003cbr\u003ewww.ausamics.com.au • support@ausamics.com • +61 412 520 598\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cstyle\u003e\n@media (max-width: 860px) {\n  .aus-hero-grid, .aus-main-grid { grid-template-columns: 1fr !important; }\n  .aus-guarantee { min-width: 100% !important; margin-top: 20px; }\n  .aus-sob-product { padding: 15px !important; }\n  .aus-sob-product h1 { font-size: 24px !important; }\n  .aus-sob-product h2 { font-size: 18px !important; }\n}\n@media (max-width: 640px) {\n  .aus-hero-section { padding: 25px !important; }\n  .aus-sob-product table th,\n  .aus-sob-product table td { padding: 8px 6px !important; }\n}\n\u003c\/style\u003e","brand":"Ausamics Life Science","offers":[{"title":"100 g","offer_id":48388166713599,"sku":"AS-1245-100G","price":55.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":48388166746367,"sku":"AS-1245-500G","price":145.0,"currency_code":"AUD","in_stock":true},{"title":"2.5 kg","offer_id":48388166779135,"sku":"AS-1245-2.5KG","price":580.0,"currency_code":"AUD","in_stock":true},{"title":"10 kg","offer_id":48388166811903,"sku":"AS-1245-10KG","price":1950.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/Hanahan_s_Broth.jpg?v=1775878704","url":"https:\/\/ausamics.com.au\/products\/hanahan-s-broth","provider":"AuSaMicS","version":"1.0","type":"link"}