{"product_id":"indigo-carmine-indigotine-e-132-asd-639","title":"Indigo Carmine (E132) – Food Grade Colourant, CI 73015, CAS 860-22-0 | ASD-639","description":"\u003c!--\nProduct: Indigo Carmine (Indigotine, E 132), C.I. 73015, CAS 860-22-0\nCatalogue No: ASD-639\nManufacturer: Ausamics Pty Ltd, Melbourne, Australia\nCategory: Biological stains and dyes\nPrimary use: Histological counterstain, redox and alkaline pH indicator, E 132 reference colour\n--\u003e\n\u003cscript type=\"application\/ld+json\"\u003e\n{\"@context\": \"https:\/\/schema.org\", \"@type\": \"Product\", \"name\": \"Indigo Carmine (Indigotine, E 132), C.I. 73015\", \"sku\": \"ASD-639\", \"mpn\": \"ASD-639\", \"category\": \"Biological Stains and Dyes\", \"alternateName\": [\"Indigotine\", \"E 132\", \"Acid Blue 74\", \"FD\u0026C Blue No. 2\", \"CI Food Blue 1\", \"Indigo-5,5'-disulfonic acid disodium salt\"], \"description\": \"Indigo carmine (CAS 860-22-0, C.I. 73015), dark blue water-soluble indigoid dye with minimum 85 % total dye content. Used as a histological counterstain, redox and alkaline pH indicator, and E 132 reference colour.\", \"brand\": {\"@type\": \"Brand\", \"name\": \"Ausamics\"}, \"manufacturer\": {\"@type\": \"Organization\", \"name\": \"Ausamics Pty Ltd\", \"url\": \"https:\/\/www.ausamics.com.au\"}, \"additionalProperty\": [{\"@type\": \"PropertyValue\", \"name\": \"CAS number\", \"value\": \"860-22-0\"}, {\"@type\": \"PropertyValue\", \"name\": \"Colour Index number\", \"value\": \"73015\"}, {\"@type\": \"PropertyValue\", \"name\": \"EC number\", \"value\": \"212-728-8\"}, {\"@type\": \"PropertyValue\", \"name\": \"Molecular formula\", \"value\": \"C16H8N2Na2O8S2\"}, {\"@type\": \"PropertyValue\", \"name\": \"Molecular weight\", \"value\": \"466.35 g\/mol\"}, {\"@type\": \"PropertyValue\", \"name\": \"Dye content\", \"value\": \"Min. 85 %\"}, {\"@type\": \"PropertyValue\", \"name\": \"Absorption maximum\", \"value\": \"approx. 610 nm (water)\"}]}\n\u003c\/script\u003e\n\u003cscript type=\"application\/ld+json\"\u003e\n{\"@context\": \"https:\/\/schema.org\", \"@type\": \"FAQPage\", \"mainEntity\": [{\"@type\": \"Question\", \"name\": \"What is the difference between indigo carmine and indigo?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Indigo is insoluble in water. Indigo carmine is indigo with two sulfonate groups added, sold as the disodium salt, so it dissolves in water and works as an acid dye.\"}}, {\"@type\": \"Question\", \"name\": \"Is this the same as E 132 or FD\u0026C Blue No. 2?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Yes. Indigo carmine, indigotine, E 132 and FD\u0026C Blue No. 2 are names for the same colour. This batch meets the purity limits of the E 132 specification on the manufacturer's certificate.\"}}, {\"@type\": \"Question\", \"name\": \"How do I make a stock solution?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Dissolve 0.1–1.0 g in 100 mL of purified water to make a 0.1–1 % w\/v solution. Solubility is about 10 g\/L at 25 °C, so warm gently for stronger solutions. Store in an amber bottle and prepare fresh when colour intensity matters.\"}}, {\"@type\": \"Question\", \"name\": \"Why did my solution turn yellow?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Indigo carmine turns yellow in two cases: when it is reduced (for example by glucose in alkaline solution) and above about pH 12. Shaking a reduced solution with air restores the blue colour.\"}}, {\"@type\": \"Question\", \"name\": \"What dye content does this product have?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"The specification is at least 85 % total dye content on a dried basis. The current batch tested at 90.72 %. A batch COA is available on request.\"}}]}\n\u003c\/script\u003e\n\u003cstyle\u003e\n.aus-nutrient-product{font-family:\"Segoe UI\",system-ui,-apple-system,Arial,sans-serif;color:#1d2935;max-width:1200px;margin:0 auto;line-height:1.6;font-size:15px}\n.aus-nutrient-product *{box-sizing:border-box}\n.aus-nutrient-product .tab-radio{position:absolute;opacity:0;pointer-events:none}\n.aus-nutrient-product .tab-labels{display:flex;flex-wrap:wrap;gap:6px;border-bottom:2px solid #2d5a3d;margin:0 0 0 0}\n.aus-nutrient-product .tab-labels label{cursor:pointer;padding:11px 20px;background:#f0f4f1;color:#2d5a3d;font-weight:600;border-radius:10px 10px 0 0;border:1px solid #d8e1e8;border-bottom:none;font-size:14px}\n.aus-nutrient-product .tab-panel{display:none;padding:26px 4px 10px}\n#asd639-t1:checked~.tab-labels label[for=asd639-t1],#asd639-t2:checked~.tab-labels label[for=asd639-t2],#asd639-t3:checked~.tab-labels label[for=asd639-t3],#asd639-t4:checked~.tab-labels 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.scale-lbl{display:flex;justify-content:space-between;font-size:12px;color:#52606d}\n.aus-nutrient-product .swatch{display:inline-block;width:14px;height:14px;border-radius:3px;vertical-align:-2px;margin-right:6px;border:1px solid rgba(0,0,0,.15)}\n.aus-nutrient-product .ghs{display:flex;align-items:center;gap:14px;margin:6px 0 10px}\n.aus-nutrient-product .ghs svg{width:64px;height:64px;flex:none}\n\u003c\/style\u003e\n\u003cdiv class=\"aus-nutrient-product\"\u003e\n\u003cinput type=\"radio\" name=\"asd639-tabs\" id=\"asd639-t1\" class=\"tab-radio\" checked\u003e\n\u003cinput type=\"radio\" name=\"asd639-tabs\" id=\"asd639-t2\" class=\"tab-radio\"\u003e\n\u003cinput type=\"radio\" name=\"asd639-tabs\" id=\"asd639-t3\" class=\"tab-radio\"\u003e\n\u003cinput type=\"radio\" name=\"asd639-tabs\" id=\"asd639-t4\" class=\"tab-radio\"\u003e\n\u003cdiv class=\"tab-labels\"\u003e\n\u003clabel for=\"asd639-t1\"\u003eProduct Description\u003c\/label\u003e\u003clabel for=\"asd639-t2\"\u003eTechnical Data\u003c\/label\u003e\u003clabel for=\"asd639-t3\"\u003eSafety Data\u003c\/label\u003e\u003clabel for=\"asd639-t4\"\u003eQuality \u0026amp; COA\u003c\/label\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"tab-panels\"\u003e\n\n\u003cdiv class=\"tab-panel p1\"\u003e\n\u003cdiv class=\"hero\"\u003e\u003cdiv class=\"hero-grid\"\u003e\n\u003cdiv\u003e\n\u003cspan class=\"eyebrow\"\u003eBiological Stains \u0026amp; Dyes · C.I. 73015\u003c\/span\u003e\n\u003ch1\u003eIndigo Carmine (Indigotine, E 132)\u003c\/h1\u003e\n\u003cp class=\"subtitle\"\u003eWater-soluble indigoid dye for histological counterstaining, redox and alkaline pH indication, and E 132 colour reference work.\u003c\/p\u003e\n\u003cdiv class=\"badges\"\u003e\n\u003cspan class=\"badge b-solid\"\u003eCat. No. ASD-639\u003c\/span\u003e\u003cspan class=\"badge b-outline\"\u003eCAS 860-22-0\u003c\/span\u003e\u003cspan class=\"badge b-outline\"\u003eDye content ≥ 85 %\u003c\/span\u003e\u003cspan class=\"badge b-green\"\u003eλmax ≈ 610 nm\u003c\/span\u003e\u003cspan class=\"badge b-gold\"\u003eFood-grade E 132\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"note-green\"\u003e\n\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Indigotine, Acid Blue 74, CI Food Blue 1, FD\u0026amp;C Blue No. 2, indigo-5,5′-disulfonic acid disodium salt.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"spec-card\"\u003e\n\u003ch4\u003eAt a Glance\u003c\/h4\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cspan\u003eFormula\u003c\/span\u003e\u003cstrong\u003eC₁₆H₈N₂Na₂O₈S₂\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cspan\u003eMol. weight\u003c\/span\u003e\u003cstrong\u003e466.35 g\/mol\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cspan\u003eAppearance\u003c\/span\u003e\u003cstrong\u003eDark blue powder\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cspan\u003eSolubility (water)\u003c\/span\u003e\u003cstrong\u003e≈ 10 g\/L, 25 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cspan\u003epH range\u003c\/span\u003e\u003cstrong\u003e11.6 blue → 14.0 yellow\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cspan\u003eBatch dye content\u003c\/span\u003e\u003cstrong\u003e90.72 %\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\n\u003cdiv class=\"main-grid\"\u003e\n\u003cdiv\u003e\n\u003ch3\u003eOverview\u003c\/h3\u003e\n\u003cp\u003eIndigo carmine is the disodium salt of indigo-5,5′-disulfonic acid. Adding two sulfonate groups turns insoluble indigo into a water-soluble anionic dye with an intense blue colour and an absorption maximum near 610 nm.\u003c\/p\u003e\n\u003cp\u003eThe dye is valued in the laboratory for three properties. It binds collagen and cytoplasm as an acid dye. It changes colour reversibly on reduction and oxidation. It turns from blue to yellow in strongly alkaline solution.\u003c\/p\u003e\n\u003cp class=\"muted\"\u003eFor laboratory, research, education and quality-control use. Not intended for human or veterinary therapeutic use, diagnosis, or direct consumption. Food-grade (E 132) material. For food-industry supply, contact Ausamics for suitability and regulatory documentation.\u003c\/p\u003e\n\n\u003ch3\u003eHow It Works\u003c\/h3\u003e\n\u003cp\u003e\u003cspan class=\"swatch\" style=\"background:#1f3fa3\"\u003e\u003c\/span\u003e\u003cstrong\u003eOxidised form\u003c\/strong\u003e is blue. \u003cspan class=\"swatch\" style=\"background:#e3c12b\"\u003e\u003c\/span\u003e\u003cstrong\u003eReduced (leuco) form\u003c\/strong\u003e is yellow. In alkaline glucose solution the dye passes through red intermediates, then re-oxidises to blue when shaken with air.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAlkaline pH indicator\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv class=\"scale\"\u003e\n\u003cspan style=\"background:#1f3fa3\"\u003e\u003c\/span\u003e\u003cspan style=\"background:#2c55b0\"\u003e\u003c\/span\u003e\u003cspan style=\"background:#4c7a8c\"\u003e\u003c\/span\u003e\u003cspan style=\"background:#8fa45a\"\u003e\u003c\/span\u003e\u003cspan style=\"background:#c9b636\"\u003e\u003c\/span\u003e\u003cspan style=\"background:#e3c12b\"\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"scale-lbl\"\u003e\n\u003cspan\u003epH 11.6 · blue\u003c\/span\u003e\u003cspan\u003epH 14.0 · yellow\u003c\/span\u003e\n\u003c\/div\u003e\n\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cdiv class=\"cards\"\u003e\n\u003cdiv class=\"card c1\"\u003e\n\u003cstrong\u003eHistology\u003c\/strong\u003eCounterstain in picro-indigo carmine methods, giving blue-green collagen against red or magenta nuclei.\u003c\/div\u003e\n\u003cdiv class=\"card c2\"\u003e\n\u003cstrong\u003eRedox Chemistry\u003c\/strong\u003eRedox indicator: blue when oxidised, yellow in the reduced leuco form. Used in oxygen-sensitive reactions.\u003c\/div\u003e\n\u003cdiv class=\"card c3\"\u003e\n\u003cstrong\u003eAlkaline pH Indicator\u003c\/strong\u003eColour change from blue to yellow between pH 11.6 and 14.0 in strongly alkaline solutions.\u003c\/div\u003e\n\u003cdiv class=\"card c1\"\u003e\n\u003cstrong\u003eEducation\u003c\/strong\u003eClassic glucose–indigo carmine “traffic light” demonstration of oxidation and reduction.\u003c\/div\u003e\n\u003cdiv class=\"card c2\"\u003e\n\u003cstrong\u003eFood Colour QC\u003c\/strong\u003eReference material for E 132 identification, spectrophotometry and colour-matching work.\u003c\/div\u003e\n\u003cdiv class=\"card c3\"\u003e\n\u003cstrong\u003eMicroscopy \u0026amp; Tracing\u003c\/strong\u003eWater-soluble blue dye for contrast, tracing and visualisation in research models.\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch3\u003eAdvantages \u0026amp; Considerations\u003c\/h3\u003e\n\u003cdiv class=\"two\"\u003e\n\u003cdiv class=\"adv\"\u003e\n\u003cstrong\u003eAdvantages\u003c\/strong\u003e\u003cul\u003e\n\u003cli\u003eHigh dye content: specification ≥ 85 %, current batch 90.72 %\u003c\/li\u003e\n\u003cli\u003eMeets the E 132 purity limits on the manufacturer's batch certificate\u003c\/li\u003e\n\u003cli\u003eWater-soluble and easy to prepare as aqueous stock\u003c\/li\u003e\n\u003cli\u003eReversible colour change for redox teaching and testing\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"con\"\u003e\n\u003cstrong\u003eConsiderations\u003c\/strong\u003e\u003cul\u003e\n\u003cli\u003eLight sensitive: store powder and solutions in the dark\u003c\/li\u003e\n\u003cli\u003eFades in strong oxidants (e.g. hypochlorite) and reducing conditions\u003c\/li\u003e\n\u003cli\u003eLimited solubility (≈ 10 g\/L); warm gently for stronger stocks\u003c\/li\u003e\n\u003cli\u003eHarmful if swallowed (H302); wear gloves when handling\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch3\u003eSpecification Summary\u003c\/h3\u003e\n\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhysical appearance\u003c\/td\u003e\n\u003ctd\u003eBlue powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTotal dye content (dried basis, 105 ± 1 °C, 2 h)\u003c\/td\u003e\n\u003ctd\u003eMin. 85 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLoss on drying at 135 °C, plus chlorides and sulfates (as sodium salts)\u003c\/td\u003e\n\u003ctd\u003eMax. 15 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater-insoluble matter\u003c\/td\u003e\n\u003ctd\u003eMax. 0.2 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCombined ether extracts\u003c\/td\u003e\n\u003ctd\u003eMax. 0.2 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubsidiary dyes\u003c\/td\u003e\n\u003ctd\u003eMax. 1 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIsatin sulfonic acid\u003c\/td\u003e\n\u003ctd\u003eMax. 0.5 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLead (Pb)\u003c\/td\u003e\n\u003ctd\u003eMax. 2 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eArsenic (As)\u003c\/td\u003e\n\u003ctd\u003eMax. 3 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeavy metals\u003c\/td\u003e\n\u003ctd\u003eMax. 40 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003ch3\u003eFrequently Asked Questions\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eWhat is the difference between indigo carmine and indigo?\u003c\/strong\u003e\u003cbr\u003eIndigo is insoluble in water. Indigo carmine is indigo with two sulfonate groups added, sold as the disodium salt, so it dissolves in water and works as an acid dye.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIs this the same as E 132 or FD\u0026amp;C Blue No. 2?\u003c\/strong\u003e\u003cbr\u003eYes. Indigo carmine, indigotine, E 132 and FD\u0026amp;C Blue No. 2 are names for the same colour. This batch meets the purity limits of the E 132 specification on the manufacturer's certificate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHow do I make a stock solution?\u003c\/strong\u003e\u003cbr\u003eDissolve 0.1–1.0 g in 100 mL of purified water to make a 0.1–1 % w\/v solution. Solubility is about 10 g\/L at 25 °C, so warm gently for stronger solutions. Store in an amber bottle and prepare fresh when colour intensity matters.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhy did my solution turn yellow?\u003c\/strong\u003e\u003cbr\u003eIndigo carmine turns yellow in two cases: when it is reduced (for example by glucose in alkaline solution) and above about pH 12. Shaking a reduced solution with air restores the blue colour.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhat dye content does this product have?\u003c\/strong\u003e\u003cbr\u003eThe specification is at least 85 % total dye content on a dried basis. The current batch tested at 90.72 %. A batch COA is available on request.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003cdiv\u003e\n\u003cdiv class=\"side-box side-white\"\u003e\n\u003ch4\u003eTechnical Specifications\u003c\/h4\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalogue No.\u003c\/td\u003e\n\u003ctd\u003eASD-639\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eC.I. number\u003c\/td\u003e\n\u003ctd\u003e73015\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCAS \/ EC\u003c\/td\u003e\n\u003ctd\u003e860-22-0 \/ 212-728-8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE number\u003c\/td\u003e\n\u003ctd\u003eE 132\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormula\u003c\/td\u003e\n\u003ctd\u003eC₁₆H₈N₂Na₂O₈S₂\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMol. weight\u003c\/td\u003e\n\u003ctd\u003e466.35 g\/mol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDye content\u003c\/td\u003e\n\u003ctd\u003eMin. 85 % (dried basis)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eλmax\u003c\/td\u003e\n\u003ctd\u003e≈ 610 nm (water)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e15–25 °C, dry, dark, tightly closed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDocuments\u003c\/td\u003e\n\u003ctd\u003eTDS, SDS; batch COA on request\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"side-box side-green\"\u003e\n\u003ch4\u003eApplications\u003c\/h4\u003e\n\u003cul style=\"margin:0;padding-left:18px;font-size:14px\"\u003e\n\u003cli\u003ePicro-indigo carmine counterstain\u003c\/li\u003e\n\u003cli\u003eRedox indicator\u003c\/li\u003e\n\u003cli\u003eAlkaline pH indicator\u003c\/li\u003e\n\u003cli\u003eChemistry teaching demonstrations\u003c\/li\u003e\n\u003cli\u003eE 132 reference and QC\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"side-box side-gold\"\u003e\n\u003ch4\u003eStock Solution (1 % w\/v)\u003c\/h4\u003e\n\u003col class=\"steps\"\u003e\n\u003cli\u003eWeigh 1.0 g of indigo carmine.\u003c\/li\u003e\n\u003cli\u003eAdd to about 80 mL of purified water.\u003c\/li\u003e\n\u003cli\u003eStir, warming gently to 40–50 °C if needed, until dissolved.\u003c\/li\u003e\n\u003cli\u003eMake up to 100 mL with purified water.\u003c\/li\u003e\n\u003cli\u003eFilter if required. Store in an amber bottle at room temperature.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"side-box side-white\"\u003e\n\u003ch4\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eStore at 15–25 °C in a dry place, tightly closed and protected from light. Use before the expiry date on the label. Prepare working solutions fresh when colour intensity is critical.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"footer-note\"\u003eAusamics Pty Ltd provides this information in good faith. Users are responsible for validating this product for their own methods and regulatory requirements. Ausamics Pty Ltd does not warrant suitability for any specific application unless expressly agreed in writing.\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"tab-panel p2\"\u003e\n\u003cdiv class=\"doc-head\"\u003e\n\u003ch2\u003eTechnical Data Sheet — Indigo Carmine\u003c\/h2\u003e\n\u003cspan\u003eTDS-ASD-639 · Rev 1.0 · November 2026\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"sec\"\u003e1. Identification\u003c\/div\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003eChemical name\u003c\/td\u003e\n\u003ctd\u003eDisodium 3,3′-dioxo-2,2′-bi-indolylidene-5,5′-disulfonate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSynonyms\u003c\/td\u003e\n\u003ctd\u003eIndigotine; Indigo-5,5′-disulfonic acid disodium salt; Acid Blue 74; CI Food Blue 1; FD\u0026amp;C Blue No. 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eC.I. number\u003c\/td\u003e\n\u003ctd\u003e73015\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE number\u003c\/td\u003e\n\u003ctd\u003eE 132\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCAS number\u003c\/td\u003e\n\u003ctd\u003e860-22-0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEC number\u003c\/td\u003e\n\u003ctd\u003e212-728-8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMolecular formula\u003c\/td\u003e\n\u003ctd\u003eC₁₆H₈N₂Na₂O₈S₂\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMolecular weight\u003c\/td\u003e\n\u003ctd\u003e466.35 g\/mol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDye class\u003c\/td\u003e\n\u003ctd\u003eIndigoid, anionic (acid) dye\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"sec\"\u003e2. Intended Use\u003c\/div\u003e\n\u003cp\u003eHistological counterstain, redox indicator, alkaline pH indicator, teaching reagent and E 132 reference colour. For laboratory, research, education and quality-control use. Not intended for human or veterinary therapeutic use, diagnosis, or direct consumption.\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003e3. Mode of Action\u003c\/div\u003e\n\u003cp\u003eThe two sulfonate groups make the dye anionic, so it binds positively charged tissue proteins such as collagen in acidic stains. The central indigoid chromophore is reversibly reduced to a yellow leuco form, and above about pH 12 the dye shifts from blue to yellow.\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003e4. Physical \u0026amp; Chemical Properties\u003c\/div\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance\u003c\/td\u003e\n\u003ctd\u003eDark blue powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColour of aqueous solution\u003c\/td\u003e\n\u003ctd\u003eBlue\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAbsorption maximum (λmax)\u003c\/td\u003e\n\u003ctd\u003eapprox. 610 nm in water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolubility\u003c\/td\u003e\n\u003ctd\u003eWater: approx. 10 g\/L at 25 °C; slightly soluble in ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH indicator range\u003c\/td\u003e\n\u003ctd\u003epH 11.6 (blue) to 14.0 (yellow)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRedox behaviour\u003c\/td\u003e\n\u003ctd\u003eReduced to a yellow leuco form; re-oxidised to blue by air\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLight sensitivity\u003c\/td\u003e\n\u003ctd\u003eLight sensitive; protect solutions and powder from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"sec\"\u003e5. Specifications\u003c\/div\u003e\n\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhysical appearance\u003c\/td\u003e\n\u003ctd\u003eBlue powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTotal dye content (dried basis, 105 ± 1 °C, 2 h)\u003c\/td\u003e\n\u003ctd\u003eMin. 85 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLoss on drying at 135 °C, plus chlorides and sulfates (as sodium salts)\u003c\/td\u003e\n\u003ctd\u003eMax. 15 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater-insoluble matter\u003c\/td\u003e\n\u003ctd\u003eMax. 0.2 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCombined ether extracts\u003c\/td\u003e\n\u003ctd\u003eMax. 0.2 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubsidiary dyes\u003c\/td\u003e\n\u003ctd\u003eMax. 1 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIsatin sulfonic acid\u003c\/td\u003e\n\u003ctd\u003eMax. 0.5 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLead (Pb)\u003c\/td\u003e\n\u003ctd\u003eMax. 2 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eArsenic (As)\u003c\/td\u003e\n\u003ctd\u003eMax. 3 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeavy metals\u003c\/td\u003e\n\u003ctd\u003eMax. 40 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"sec\"\u003e6. Solution Preparation\u003c\/div\u003e\n\u003cp\u003eStock solution (1 % w\/v): dissolve 1.0 g in about 80 mL purified water, warming gently if needed, and make up to 100 mL. Dilute for use according to your method. Store in amber glass.\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003e7. Storage \u0026amp; Shelf Life\u003c\/div\u003e\n\u003cp\u003ePowder: 15–25 °C, dry, tightly closed, protected from light. Use before the expiry date on the label.\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003e8. References\u003c\/div\u003e\n\u003col class=\"muted\"\u003e\n\u003cli\u003eCommission Regulation (EU) No 231\/2012, Annex: E 132 Indigotine, Indigo carmine. Specifications for food additives.\u003c\/li\u003e\n\u003cli\u003eFAO\/WHO JECFA. Indigotine: Combined Compendium of Food Additive Specifications, Monograph 1.\u003c\/li\u003e\n\u003cli\u003eEFSA ANS Panel (2014). Scientific Opinion on the re-evaluation of indigo carmine (E 132) as a food additive. EFSA Journal 12(7):3768.\u003c\/li\u003e\n\u003cli\u003eHorobin R.W., Kiernan J.A. (eds) (2002). Conn's Biological Stains, 10th ed. BIOS Scientific Publishers, Oxford.\u003c\/li\u003e\n\u003cli\u003eSuvarna S.K., Layton C., Bancroft J.D. (eds) (2019). Bancroft's Theory and Practice of Histological Techniques, 8th ed. Elsevier.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"tab-panel p3\"\u003e\n\u003cdiv class=\"doc-head\"\u003e\n\u003ch2\u003eSafety Data Summary — Indigo Carmine\u003c\/h2\u003e\n\u003cspan\u003eSDS-ASD-639 · Rev 1.0 · November 2026 · Full 16-section SDS available on request\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"sec\"\u003eHazard Classification (GHS)\u003c\/div\u003e\n\u003cdiv class=\"ghs\"\u003e\n\u003csvg viewbox=\"0 0 100 100\" role=\"img\" aria-label=\"GHS07 exclamation mark pictogram\"\u003e\u003cpolygon points=\"50,3 97,50 50,97 3,50\" fill=\"#fff\" stroke=\"#e00000\" stroke-width=\"7\"\u003e\u003c\/polygon\u003e\u003crect x=\"45\" y=\"24\" width=\"10\" height=\"36\" rx=\"4\" fill=\"#000\"\u003e\u003c\/rect\u003e\u003ccircle cx=\"50\" cy=\"71\" r=\"6\" fill=\"#000\"\u003e\u003c\/circle\u003e\u003c\/svg\u003e\u003cdiv\u003e\n\u003cstrong\u003eSignal word: Warning\u003c\/strong\u003e\u003cbr\u003eAcute toxicity, oral, Category 4\u003cbr\u003e\u003cstrong\u003eH302\u003c\/strong\u003e Harmful if swallowed.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003ePrecautionary statements:\u003c\/strong\u003e P264 Wash hands thoroughly after handling. P270 Do not eat, drink or smoke when using this product. P301+P312 If swallowed: call a Poisons Information Centre or doctor if you feel unwell. P330 Rinse mouth. P501 Dispose of contents and container in accordance with local regulations.\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003eHandling \u0026amp; Protection\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003eAvoid creating and breathing dust. Use with adequate ventilation.\u003c\/li\u003e\n\u003cli\u003eWear nitrile gloves, laboratory coat and safety glasses.\u003c\/li\u003e\n\u003cli\u003eThe dye stains skin and surfaces; clean spills promptly with water.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv class=\"sec\"\u003eFirst Aid\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eInhalation:\u003c\/strong\u003e Move to fresh air.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSkin:\u003c\/strong\u003e Wash with soap and water.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEyes:\u003c\/strong\u003e Rinse with water for several minutes. Seek medical advice if irritation persists.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIngestion:\u003c\/strong\u003e Rinse mouth. Call a Poisons Information Centre or doctor if you feel unwell.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"muted\"\u003ePoisons Information Centre (Australia): 13 11 26 · Emergency: 000\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003eStorage \u0026amp; Disposal\u003c\/div\u003e\n\u003cp\u003eStore at 15–25 °C, dry, tightly closed and protected from light. Dispose of according to local regulations.\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003eTransport\u003c\/div\u003e\n\u003cp\u003eNot regulated as dangerous goods for road (ADG), sea (IMDG) or air (IATA) transport.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"tab-panel p4\"\u003e\n\u003cdiv class=\"doc-head\"\u003e\n\u003ch2\u003eQuality \u0026amp; Certificate of Analysis\u003c\/h2\u003e\n\u003cspan\u003eASD-639 · Batch-specific COA available on request\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cp\u003eEach batch is supplied with traceable manufacturer test data. A batch-specific Certificate of Analysis is available on request. Please quote the batch number printed on the label.\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003eTypical Batch Results (Batch ZC30U)\u003c\/div\u003e\n\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eTest\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eResult\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePhysical appearance\u003c\/td\u003e\n\u003ctd\u003eBlue powder\u003c\/td\u003e\n\u003ctd\u003eComplies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTotal dye content (dried basis, 105 ± 1 °C, 2 h)\u003c\/td\u003e\n\u003ctd\u003eMin. 85 %\u003c\/td\u003e\n\u003ctd\u003e90.72 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLoss on drying at 135 °C, plus chlorides and sulfates (as sodium salts)\u003c\/td\u003e\n\u003ctd\u003eMax. 15 %\u003c\/td\u003e\n\u003ctd\u003e8.97 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater-insoluble matter\u003c\/td\u003e\n\u003ctd\u003eMax. 0.2 %\u003c\/td\u003e\n\u003ctd\u003e0.109 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCombined ether extracts\u003c\/td\u003e\n\u003ctd\u003eMax. 0.2 %\u003c\/td\u003e\n\u003ctd\u003e0.098 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubsidiary dyes\u003c\/td\u003e\n\u003ctd\u003eMax. 1 %\u003c\/td\u003e\n\u003ctd\u003e\u0026lt; 1 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIsatin sulfonic acid\u003c\/td\u003e\n\u003ctd\u003eMax. 0.5 %\u003c\/td\u003e\n\u003ctd\u003e\u0026lt; 0.5 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLead (Pb)\u003c\/td\u003e\n\u003ctd\u003eMax. 2 ppm\u003c\/td\u003e\n\u003ctd\u003e\u0026lt; 2 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eArsenic (As)\u003c\/td\u003e\n\u003ctd\u003eMax. 3 ppm\u003c\/td\u003e\n\u003ctd\u003e\u0026lt; 3 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeavy metals\u003c\/td\u003e\n\u003ctd\u003eMax. 40 ppm\u003c\/td\u003e\n\u003ctd\u003e\u0026lt; 40 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"muted\"\u003eResults as reported on the manufacturer's certificate for batch ZC30U. Manufactured 30\/03\/2026; expiry 29\/03\/2031.\u003c\/p\u003e\n\u003cdiv class=\"footer-note\"\u003eThe results reported in a Certificate of Analysis apply only to the batch identified on it. A certificate may not be reproduced except in full without prior written approval from Ausamics Pty Ltd. The user is responsible for determining the suitability of this product for their intended application and for ensuring its proper use, handling, and storage. Ausamics Pty Ltd does not warrant the suitability or performance of this product for any specific application unless expressly agreed in writing. To the extent permitted by applicable law, Ausamics Pty Ltd accepts no liability for loss or damage arising from improper use or handling of this product.\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"AuSaMicS","offers":[{"title":"25 g","offer_id":51031903633663,"sku":"ASD-639-25G","price":27.0,"currency_code":"AUD","in_stock":true},{"title":"100 g","offer_id":51031903699199,"sku":"ASD-639-100G","price":45.0,"currency_code":"AUD","in_stock":true},{"title":"250 g","offer_id":51031903731967,"sku":"ASD-639-250G","price":79.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":51031903764735,"sku":"ASD-639-500G","price":117.0,"currency_code":"AUD","in_stock":true},{"title":"1 kg","offer_id":51031903797503,"sku":"ASD-639-1KG","price":189.0,"currency_code":"AUD","in_stock":true},{"title":"5 kg","offer_id":51031903830271,"sku":"ASD-639-5KG","price":749.0,"currency_code":"AUD","in_stock":true}],"url":"https:\/\/ausamics.com.au\/products\/indigo-carmine-indigotine-e-132-asd-639","provider":"AuSaMicS","version":"1.0","type":"link"}