{"product_id":"indole-nitrite-medium-trypticase-nitrate-broth","title":"Indole Nitrite Medium (AS-1250) | Trypticase Nitrate Broth","description":"\u003cdiv style=\"font-family: 'Segoe UI', system-ui, -apple-system, sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\" class=\"aus-nutrient-product\"\u003e\n    \u003cdiv style=\"background: linear-gradient(135deg, #f5f3ff 0%, #ede9fe 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.06); border: 1px solid #ddd6fe; border-left: 8px solid #7c3aed;\" class=\"aus-hero-section\"\u003e\n        \u003cdiv class=\"aus-hero-grid\" style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\"\u003e\n            \u003cdiv\u003e\n                \u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #5b21b6; font-weight: bold; margin-bottom: 12px; background: #fff; padding: 6px 12px; border-radius: 20px; border: 1px solid #ddd6fe;\"\u003eAuSaMicS Life Science • Biochemical Differentiation\u003c\/div\u003e\n                \u003ch1 style=\"color: #2e1065; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003eIndole Nitrite Medium\u003c\/h1\u003e\n                \u003ch2 style=\"color: #4c1d95; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: 0.9;\"\u003eTrypticase Nitrate Broth for ID\u003c\/h2\u003e\n                \u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #2e1065; max-width: 600px; font-weight: 500;\"\u003eA dual-purpose \u003cstrong\u003ediagnostic medium\u003c\/strong\u003e used for the identification of microorganisms based on their ability to produce indole and reduce nitrate to nitrite. Highly effective for the differentiation of non-fermentative bacilli and \u003ci\u003eEnterobacteriaceae\u003c\/i\u003e.\u003c\/p\u003e\n                \u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n                    \u003cspan style=\"background: #7c3aed; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAS-1250\u003c\/span\u003e \n                    \u003cspan style=\"background: #fff; border: 2px solid #7c3aed; color: #7c3aed; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e⚗️ Dual Test Formula\u003c\/span\u003e \n                    \u003cspan style=\"background: #5b21b6; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e✓ AOAC \/ BAM Compliant\u003c\/span\u003e \n                    \u003cspan style=\"background: #f3f4f6; color: #374151; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🔬 High Clarity\u003c\/span\u003e\n                \u003c\/div\u003e\n                \u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #6d28d9;\"\u003e\n                    \u003cspan\u003e\u003cstrong\u003eTests:\u003c\/strong\u003e Indole \u0026amp; Nitrate Reduction\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eIncubation:\u003c\/strong\u003e 35°C \/ 18-48h\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003ePrep:\u003c\/strong\u003e 25.0g\/L Yield\u003c\/span\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"aus-guarantee\" style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 280px; border: 1px solid #ddd6fe;\"\u003e\n                \u003ch4 style=\"color: #2e1065; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003e ID Specialist\u003c\/h4\u003e\n                \u003cdiv style=\"font-size: 14px; color: #4c1d95; text-align: center; line-height: 1.5;\"\u003e\n                    \u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eTryptophan Rich\u003c\/strong\u003e - Optimal for indole production\u003c\/div\u003e\n                    \u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eStandardized Nitrate\u003c\/strong\u003e - Reliable reduction assays\u003c\/div\u003e\n                    \u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eBroad Application\u003c\/strong\u003e - Clinically validated base\u003c\/div\u003e\n                    \u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eClear Interpretation\u003c\/strong\u003e - Rapid color development\u003c\/div\u003e\n                \u003c\/div\u003e\n                \u003cdiv style=\"margin-top: 20px; padding: 15px; background: #f5f3ff; border-radius: 12px; text-align: center; border: 1px solid #ddd6fe;\"\u003e\n                    \u003cdiv style=\"color: #5b21b6; font-weight: 600; font-size: 14px;\"\u003eDifferential Biochemistry\u003c\/div\u003e\n                    \u003cdiv style=\"color: #6d28d9; font-size: 13px; margin-top: 5px;\"\u003ePure Culture Identification\u003c\/div\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv class=\"aus-main-grid\" style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\"\u003e\n        \u003cdiv\u003e\n            \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n                \u003ch3 style=\"color: #2e1065; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #7c3aed;\"\u003e🧪 Biochemical Matrix\u003c\/h3\u003e\n                \u003cdiv style=\"background: #f9fafb; padding: 20px; border-radius: 16px; border-left: 5px solid #7c3aed; margin-bottom: 20px;\"\u003e\n                    \u003ch4 style=\"color: #2e1065; margin: 0 0 15px 0; font-size: 16px;\"\u003e📋 Formulation (per Liter)\u003c\/h4\u003e\n                    \u003cdiv style=\"overflow-x: auto;\"\u003e\n                        \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #e5e7eb;\"\u003e\n                            \u003cthead style=\"background: #f3f4f6;\"\u003e\n                                \u003ctr\u003e\n                                    \u003cth style=\"padding: 12px; text-align: left;\"\u003eComponent\u003c\/th\u003e\n                                    \u003cth style=\"padding: 12px; text-align: left;\"\u003eConcentration\u003c\/th\u003e\n                                    \u003cth style=\"padding: 12px; text-align: left;\"\u003eRole\u003c\/th\u003e\n                                \u003c\/tr\u003e\n                            \u003c\/thead\u003e\n                            \u003ctbody\u003e\n                                \u003ctr style=\"border-bottom: 1px solid #f3f4f6;\"\u003e\n                                    \u003ctd style=\"padding: 10px; font-weight: 600;\"\u003ePancreatic Digest of Casein\u003c\/td\u003e\n                                    \u003ctd style=\"padding: 10px;\"\u003e20.0 g\u003c\/td\u003e\n                                    \u003ctd style=\"padding: 10px;\"\u003eHigh tryptophan source\u003c\/td\u003e\n                                \u003c\/tr\u003e\n                                \u003ctr style=\"border-bottom: 1px solid #f3f4f6;\"\u003e\n                                    \u003ctd style=\"padding: 10px; font-weight: 600;\"\u003ePotassium Nitrate ($KNO_3$)\u003c\/td\u003e\n                                    \u003ctd style=\"padding: 10px;\"\u003e1.0 g\u003c\/td\u003e\n                                    \u003ctd style=\"padding: 10px;\"\u003eSubstrate for nitrate reduction\u003c\/td\u003e\n                                \u003c\/tr\u003e\n                                \u003ctr style=\"border-bottom: 1px solid #f3f4f6;\"\u003e\n                                    \u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eDextrose\u003c\/td\u003e\n                                    \u003ctd style=\"padding: 10px;\"\u003e1.0 g\u003c\/td\u003e\n                                    \u003ctd style=\"padding: 10px;\"\u003eEnergy source for metabolism\u003c\/td\u003e\n                                \u003c\/tr\u003e\n                                \u003ctr style=\"border-bottom: 1px solid #f3f4f6;\"\u003e\n                                    \u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eDisodium Phosphate\u003c\/td\u003e\n                                    \u003ctd style=\"padding: 10px;\"\u003e2.0 g\u003c\/td\u003e\n                                    \u003ctd style=\"padding: 10px;\"\u003eBuffering agent\u003c\/td\u003e\n                                \u003c\/tr\u003e\n                                \u003ctr\u003e\n                                    \u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eAgar (Slight concentration)\u003c\/td\u003e\n                                    \u003ctd style=\"padding: 10px;\"\u003e1.0 g\u003c\/td\u003e\n                                    \u003ctd style=\"padding: 10px;\"\u003eImproves anaerobic conditions\u003c\/td\u003e\n                                \u003c\/tr\u003e\n                            \u003c\/tbody\u003e\n                        \u003c\/table\u003e\n                    \u003c\/div\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n\n            \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n                \u003ch3 style=\"color: #2e1065; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #7c3aed;\"\u003eDiagnostic Interpretation\u003c\/h3\u003e\n                \u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(250px, 1fr)); gap: 15px;\"\u003e\n                    \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #e5e7eb; border-top: 4px solid #7c3aed;\"\u003e\n                        \u003ch4 style=\"margin: 0 0 10px 0; font-size: 16px;\"\u003eIndole Test (+)\u003c\/h4\u003e\n                        \u003cp style=\"font-size: 13px; margin: 0;\"\u003eFormation of a \u003cstrong\u003ePink-Red ring\u003c\/strong\u003e at the surface after adding Kovacs' Reagent indicates tryptophanase activity.\u003c\/p\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #e5e7eb; border-top: 4px solid #7c3aed;\"\u003e\n                        \u003ch4 style=\"margin: 0 0 10px 0; font-size: 16px;\"\u003eNitrate Reduction (+)\u003c\/h4\u003e\n                        \u003cp style=\"font-size: 13px; margin: 0;\"\u003eDevelopment of a \u003cstrong\u003eRed color\u003c\/strong\u003e after adding Sulfanilic acid and Naphthylamine confirms $NO_3$ to $NO_2$ reduction.\u003c\/p\u003e\n                    \u003c\/div\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n\n        \u003cdiv\u003e\n            \u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #ddd6fe; box-shadow: 0 4px 12px rgba(0,0,0,0.05); margin-bottom: 30px;\"\u003e\n                \u003ch3 style=\"color: #2e1065; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003e📋 Technical Profile\u003c\/h3\u003e\n                \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px;\"\u003e\n                    \u003ctbody\u003e\n                        \u003ctr style=\"border-bottom: 1px solid #f3f4f6;\"\u003e\n                            \u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f5f3ff;\"\u003eCatalogue No.\u003c\/td\u003e\n                            \u003ctd style=\"padding: 12px 8px;\"\u003eAS-1250\u003c\/td\u003e\n                        \u003c\/tr\u003e\n                        \u003ctr style=\"border-bottom: 1px solid #f3f4f6;\"\u003e\n                            \u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f5f3ff;\"\u003eFinal pH\u003c\/td\u003e\n                            \u003ctd style=\"padding: 12px 8px;\"\u003e7.2 ± 0.2\u003c\/td\u003e\n                        \u003c\/tr\u003e\n                        \u003ctr style=\"border-bottom: 1px solid #f3f4f6;\"\u003e\n                            \u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f5f3ff;\"\u003eYield\u003c\/td\u003e\n                            \u003ctd style=\"padding: 12px 8px;\"\u003e25.0g \/ Liter\u003c\/td\u003e\n                        \u003c\/tr\u003e\n                        \u003ctr\u003e\n                            \u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f5f3ff;\"\u003eAppearance\u003c\/td\u003e\n                            \u003ctd style=\"padding: 12px 8px;\"\u003eFine beige powder\u003c\/td\u003e\n                        \u003c\/tr\u003e\n                    \u003c\/tbody\u003e\n                \u003c\/table\u003e\n            \u003c\/div\u003e\n\n            \u003cdiv style=\"background: #2e1065; padding: 25px; border-radius: 18px; color: #fff; margin-bottom: 30px;\"\u003e\n                \u003ch4 style=\"color: #c4b5fd; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e⚗️ Laboratory Prep\u003c\/h4\u003e\n                \u003col style=\"list-style: none; padding: 0; margin: 0; counter-reset: steps;\"\u003e\n                    \u003cli style=\"padding: 8px 0; font-size: 14px; counter-increment: steps; position: relative; padding-left: 35px;\"\u003e\n                        \u003cspan style=\"position: absolute; left: 0; top: 8px; background: #7c3aed; color: #fff; width: 22px; height: 22px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 12px; font-weight: bold;\"\u003e1\u003c\/span\u003e Suspend \u003cstrong\u003e25.0g\u003c\/strong\u003e in 1L deionized water.\n                    \u003c\/li\u003e\n                    \u003cli style=\"padding: 8px 0; font-size: 14px; counter-increment: steps; position: relative; padding-left: 35px;\"\u003e\n                        \u003cspan style=\"position: absolute; left: 0; top: 8px; background: #7c3aed; color: #fff; width: 22px; height: 22px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 12px; font-weight: bold;\"\u003e2\u003c\/span\u003e Mix well and dispense into test tubes.\n                    \u003c\/li\u003e\n                    \u003cli style=\"padding: 8px 0; font-size: 14px; counter-increment: steps; position: relative; padding-left: 35px;\"\u003e\n                        \u003cspan style=\"position: absolute; left: 0; top: 8px; background: #7c3aed; color: #fff; width: 22px; height: 22px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 12px; font-weight: bold;\"\u003e3\u003c\/span\u003e Autoclave at \u003cstrong\u003e121°C for 15 min\u003c\/strong\u003e.\n                    \u003c\/li\u003e\n                \u003c\/ol\u003e\n            \u003c\/div\u003e\n\n            \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #ddd6fe;\"\u003e\n                \u003ch4 style=\"color: #2e1065; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e📄 Documentation\u003c\/h4\u003e\n                \u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 10px;\"\u003e\n                    \u003ca style=\"display: block; padding: 12px; background: #f5f3ff; color: #7c3aed; text-decoration: none; border-radius: 10px; text-align: center; font-size: 13px; font-weight: 600; border: 1px solid #7c3aed;\" href=\"#\"\u003e📋 View COA\u003c\/a\u003e \n                    \u003ca style=\"display: block; padding: 12px; background: #fff; color: #7c3aed; text-decoration: none; border-radius: 10px; text-align: center; font-size: 13px; font-weight: 600; border: 1px solid #7c3aed;\" href=\"#\"\u003e⚠️ SDS\u003c\/a\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Ausamics Life Science","offers":[{"title":"Default Title","offer_id":48030971560191,"sku":"AS-1250-500G","price":95.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/Indole_Nitrite_broth.jpg?v=1776044834","url":"https:\/\/ausamics.com.au\/products\/indole-nitrite-medium-trypticase-nitrate-broth","provider":"AuSaMicS","version":"1.0","type":"link"}