{"product_id":"isp-medium-1","title":"ISP Medium 1 (AS-1210) | Tryptone-Yeast Extract Broth for Streptomyces","description":"\u003cp\u003e \u003c\/p\u003e\n\u003cdiv style=\"font-family: 'Segoe UI',system-ui,-apple-system,sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\"\u003e\n\u003cdiv style=\"background: linear-gradient(135deg,#eef6e9 0%,#dfead4 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #d9e6cd; border-left: 8px solid #5a8f2b;\"\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #5a8f2b; font-weight: bold; margin-bottom: 12px; background: #fff; padding: 6px 12px; border-radius: 20px; border: 1px solid #d9e6cd;\"\u003eAuSaMicS Life Science • ISP Actinomycete Media\u003c\/div\u003e\n\u003ch1 style=\"color: #15324b; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003eISP Medium 1\u003c\/h1\u003e\n\u003ch2 style=\"color: #3d5a1a; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: .95;\"\u003eTryptone Yeast Extract Broth for Streptomyces Enrichment \u0026amp; Inoculum Preparation\u003c\/h2\u003e\n\u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #455a64; max-width: 720px; font-weight: 500;\"\u003eA simple but highly useful \u003cstrong\u003ebroth medium\u003c\/strong\u003e from the International Streptomyces Project used for inoculum preparation, enrichment, and physiological support of \u003cem\u003eStreptomyces\u003c\/em\u003e species and related actinomycetes.\u003c\/p\u003e\n\u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n\u003cspan style=\"background: #5a8f2b; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: bold; white-space: nowrap;\"\u003eAS-1251\u003c\/span\u003e \u003cspan style=\"background: #fff; border: 2px solid #5a8f2b; color: #5a8f2b; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🧫 ISP Series\u003c\/span\u003e \u003cspan style=\"background: #2d5a3d; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e✓ Streptomyces Inoculum\u003c\/span\u003e \u003cspan style=\"background: #c9a84c; color: #1a1a1a; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🧪 Taxonomy Support\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #546e3a;\"\u003e\n\u003cspan\u003e\u003cstrong\u003eType:\u003c\/strong\u003e Broth\u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003ePreparation:\u003c\/strong\u003e 8.0 g\/L\u003c\/span\u003e\u003cspan\u003e\u003cstrong\u003epH:\u003c\/strong\u003e 7.0 ± 0.2\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.10); min-width: 290px; border: 1px solid #d9e6cd;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; text-align: center; font-size: 16px; font-weight: bold;\"\u003eInoculum Specialist\u003c\/h4\u003e\n\u003cdiv style=\"font-size: 14px; color: #455a64; text-align: center; line-height: 1.5;\"\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eTryptone\u003c\/strong\u003e — peptides and nitrogen\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eYeast Extract\u003c\/strong\u003e — vitamins and trace nutrients\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eSimple Formula\u003c\/strong\u003e — reproducible inoculum base\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eISP Workflow\u003c\/strong\u003e — links to ISP 2 and ISP 4 plating\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #5a8f2b;\"\u003eOverview\u003c\/h3\u003e\n\u003cdiv style=\"background: #fff; padding: 22px; border-radius: 16px; border: 1px solid #d9e6cd;\"\u003e\n\u003cp style=\"margin: 0 0 14px 0;\"\u003eISP Medium 1, also known as Tryptone Yeast Extract Broth, is a basal broth from the International Streptomyces Project used primarily for preparation of inoculum and enrichment of \u003cem\u003eStreptomyces\u003c\/em\u003e species. Its simple composition promotes reproducible early growth without imposing overly complex nutritional variables. citeturn584042search0turn584042search7\u003c\/p\u003e\n\u003cp style=\"margin: 0 0 14px 0;\"\u003eBecause morphology and pigmentation studies on ISP agar media depend heavily on consistent inoculum quality, ISP 1 functions as the practical starting point in many taxonomic and characterization workflows. ATCC strain handling information also links Streptomyces cultivation to ISP Medium 1 followed by ISP Medium 2 agar. citeturn421530search1turn421530search11\u003c\/p\u003e\n\u003cp style=\"margin: 0;\"\u003eThis makes ISP 1 less of a final differentiation medium and more of a controlled preparatory broth within the wider ISP system.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #5a8f2b;\"\u003eTypical Composition (per Liter)\u003c\/h3\u003e\n\u003cdiv style=\"background: #f7fbf3; padding: 20px; border-radius: 16px; border-left: 5px solid #5a8f2b;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #d9e6cd;\"\u003e\n\u003cthead style=\"background: #eaf3df;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 12px; text-align: left;\"\u003eIngredient\u003c\/th\u003e\n\u003cth style=\"padding: 12px; text-align: left;\"\u003eConcentration\u003c\/th\u003e\n\u003cth style=\"padding: 12px; text-align: left;\"\u003eRole\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #d9e6cd;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eTryptone\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003e5.0 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003ePeptides, amino acids, nitrogen source\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eYeast extract\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003e3.0 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eVitamin B complex, trace factors, growth support\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"margin: 12px 0 0 0; font-size: 13px; color: #4a5568;\"\u003eFinal pH: 7.0 ± 0.2 at 25°C. Typical preparation: 8.0 g\/L. citeturn584042search0turn584042search7\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #5a8f2b;\"\u003eApplications\u003c\/h3\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d9e6cd;\"\u003e\n\u003cul style=\"margin: 0; padding-left: 20px;\"\u003e\n\u003cli\u003ePreparation of Streptomyces inoculum for ISP agar media\u003c\/li\u003e\n\u003cli\u003eGeneral enrichment and cultivation of actinomycetes\u003c\/li\u003e\n\u003cli\u003ePhysiological studies including carbohydrate-use variants when supplemented\u003c\/li\u003e\n\u003cli\u003ePre-culture before morphology and pigmentation evaluation on ISP 2\/3\/4\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #5a8f2b;\"\u003eISP Workflow Comparison\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #d9e6cd;\"\u003e\n\u003cthead style=\"background: #15324b; color: #fff;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 12px; text-align: left;\"\u003eMedium\u003c\/th\u003e\n\u003cth style=\"padding: 12px; text-align: left;\"\u003eRole\u003c\/th\u003e\n\u003cth style=\"padding: 12px; text-align: left;\"\u003eBest Use\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #f7fbf3; border-bottom: 1px solid #d9e6cd;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: bold; color: #5a8f2b;\"\u003eISP 1\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eBroth inoculum medium\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003ePre-growth before plating and characterization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d9e6cd;\"\u003e\n\u003ctd style=\"padding: 10px;\"\u003eISP 2\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eYeast-malt agar\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eMorphology, aerial mycelium, pigment expression\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d9e6cd;\"\u003e\n\u003ctd style=\"padding: 10px;\"\u003eISP 3\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eOatmeal agar\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eSporulation and colony character comparison\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px;\"\u003eISP 4\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eInorganic salts starch agar\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eStarch utilization and characterization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #d9e6cd; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #d9e6cd;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f7fbf3;\"\u003eCat. No.\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px;\"\u003eAS-1251\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d9e6cd;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f7fbf3;\"\u003eMedium Type\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px;\"\u003eBroth\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d9e6cd;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f7fbf3;\"\u003ePreparation\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px;\"\u003e8.0 g\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d9e6cd;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f7fbf3;\"\u003eFinal pH\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px;\"\u003e7.0 ± 0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d9e6cd;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f7fbf3;\"\u003eSterilization\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px;\"\u003e121°C for 15 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f7fbf3;\"\u003eTypical Use\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px;\"\u003eStreptomyces inoculum and enrichment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #5a8f2b; padding: 25px; border-radius: 18px; color: #fff; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #f0ffe3; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003ePreparation Protocol\u003c\/h4\u003e\n\u003col style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 8px 0; position: relative; padding-left: 35px;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; top: 8px; background: #fff; color: #5a8f2b; width: 22px; height: 22px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 12px; font-weight: bold;\"\u003e1\u003c\/span\u003eSuspend 8.0 g in 1 L purified water.\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; position: relative; padding-left: 35px;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; top: 8px; background: #fff; color: #5a8f2b; width: 22px; height: 22px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 12px; font-weight: bold;\"\u003e2\u003c\/span\u003eHeat if necessary until dissolved.\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; position: relative; padding-left: 35px;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; top: 8px; background: #fff; color: #5a8f2b; width: 22px; height: 22px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 12px; font-weight: bold;\"\u003e3\u003c\/span\u003eDispense into tubes or flasks.\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; position: relative; padding-left: 35px;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; top: 8px; background: #fff; color: #5a8f2b; width: 22px; height: 22px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 12px; font-weight: bold;\"\u003e4\u003c\/span\u003eAutoclave at 121°C for 15 minutes. citeturn584042search0turn584042search7\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #d9e6cd; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 16px 0; font-size: 18px; text-align: center;\"\u003ePack Sizes \u0026amp; Suggested Pricing\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #d9e6cd;\"\u003e\n\u003ctd style=\"padding: 10px 8px; font-weight: 600;\"\u003e100 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 8px;\"\u003eAUD 28\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d9e6cd;\"\u003e\n\u003ctd style=\"padding: 10px 8px; font-weight: 600;\"\u003e250 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 8px;\"\u003eAUD 56\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d9e6cd; background: #f7fbf3;\"\u003e\n\u003ctd style=\"padding: 10px 8px; font-weight: bold; color: #5a8f2b;\"\u003e500 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 8px; font-weight: bold; color: #5a8f2b;\"\u003eAUD 92\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d9e6cd;\"\u003e\n\u003ctd style=\"padding: 10px 8px; font-weight: 600;\"\u003e1 kg\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 8px;\"\u003eAUD 155\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px 8px; font-weight: 600;\"\u003e5 kg\u003c\/td\u003e\n\u003ctd style=\"padding: 10px 8px;\"\u003eAUD 590\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #15324b; color: #fff; border-radius: 18px; padding: 28px; margin-top: 20px;\"\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1.4fr 0.8fr; gap: 24px;\"\u003e\n\u003cdiv\u003e\n\u003ch3 style=\"margin: 0 0 12px 0; font-size: 22px;\"\u003eAuSaMicS Compliance \u0026amp; Supply Notes\u003c\/h3\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 15px; color: #d6ecff;\"\u003eSupplied for laboratory and research workflows. Batch-specific documentation may include Certificate of Analysis, Technical Data Sheet, and Safety Data Sheet depending on customer requirements.\u003c\/p\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #d6ecff;\"\u003e\u003cstrong\u003eFor laboratory and research use only.\u003c\/strong\u003e Not for household, therapeutic, or pharmaceutical applications.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: rgba(255,255,255,0.08); border: 1px solid rgba(255,255,255,0.15); border-radius: 14px; padding: 18px;\"\u003e\n\u003cdiv style=\"font-size: 14px; margin-bottom: 8px;\"\u003e\u003cstrong\u003eAuSaMicS Pty Ltd\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv style=\"font-size: 14px; margin-bottom: 4px;\"\u003e31 Longview CT, Thomastown, VIC 3074, Australia\u003c\/div\u003e\n\u003cdiv style=\"font-size: 14px; margin-bottom: 4px;\"\u003eABN 56676640467\u003c\/div\u003e\n\u003cdiv style=\"font-size: 14px; margin-bottom: 4px;\"\u003ewww.ausamics.com.au\u003c\/div\u003e\n\u003cdiv style=\"font-size: 14px; margin-bottom: 4px;\"\u003esupport@ausamics.com\u003c\/div\u003e\n\u003cdiv style=\"font-size: 14px;\"\u003e+61 412 520 598\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Ausamics Life Science","offers":[{"title":"Default Title","offer_id":48030971592959,"sku":"AS-1251-500G","price":115.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/ISP_Medium_1_Tryptone_Yeast_Extract_Broth.jpg?v=1776046278","url":"https:\/\/ausamics.com.au\/products\/isp-medium-1","provider":"AuSaMicS","version":"1.0","type":"link"}