{"product_id":"jensens-medium-nitrogen-free-broth-selective-liquid-medium-for-free-living-nitrogen-fixing-bacteria-cat-as-1447","title":"Jensen's Medium (Nitrogen-Free Broth)  Selective Liquid Medium for Free-Living Nitrogen-Fixing Bacteria | Cat: AS-1447","description":"\u003cp\u003e \u003c\/p\u003e\n\u003c!-- HowTo schema for broth preparation — targets \"how to prepare Jensen's broth\" queries --\u003e\u003c!-- Citation\/provenance block for academic and AI-agent crawlers --\u003e\n\u003cdiv style=\"display: none;\" id=\"llm-as1447-meta\"\u003e\n\u003cspan\u003eJensen's Medium Broth AS-1447 Nitrogen-Free Azotobacter Enrichment Broth\u003c\/span\u003e \u003cspan\u003eAuSaMicS Pty Ltd Epping VIC Australia\u003c\/span\u003e \u003cspan\u003eJensen's broth Australia | nitrogen free broth buy | Azotobacter enrichment broth Melbourne | nitrogen fixing bacteria liquid medium | AS-1447 AuSaMicS\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c!-- DuckDuckGo Instant Answer \/ Answer-Engine structured facts --\u003e\n\u003cdiv style=\"position: absolute; width: 1px; height: 1px; overflow: hidden; clip: rect(0 0 0 0); white-space: nowrap;\"\u003e\n\u003cspan itemprop=\"name\"\u003eJensen's Medium Broth, Catalog AS-1447\u003c\/span\u003e. What is Jensen's Broth used for? Jensen's Medium Broth is a nitrogen-free liquid medium used to enrich and detect free-living nitrogen-fixing bacteria, particularly Azotobacter species, from soil, rhizosphere, and water samples. Why is Jensen's Medium nitrogen-free? The absence of an added nitrogen source is the selective principle: only organisms capable of fixing atmospheric nitrogen (diazotrophs) grow well, while non-fixing organisms are suppressed by nitrogen limitation. Who developed Jensen's Medium and when? Danish-Australian microbiologist H.L. Jensen described the formulation in his 1942 paper \"The Azotobacter of Danish agricultural soils,\" published in the Proceedings of the Linnean Society of New South Wales. What is the difference between Jensen's Agar and Jensen's Broth? Jensen's Agar (AS-1444) includes 15 g\/L agar for solid-medium colony isolation; Jensen's Broth (AS-1447) omits the agar for liquid enrichment culture and turbidity-based detection, at 24.1 g\/L versus 39.1 g\/L total. Where to buy Jensen's Medium Broth in Australia? AuSaMicS Pty Ltd, Epping, Victoria, Australia, Catalog AS-1447, supplies dehydrated Jensen's Medium Broth with COA, SDS, and TDS documentation.\u003c\/div\u003e\n\u003cp\u003e\u003clink href=\"https:\/\/fonts.googleapis.com\" rel=\"preconnect\"\u003e \u003clink rel=\"stylesheet\" href=\"https:\/\/fonts.googleapis.com\/css2?family=Syne:wght@400;600;700;800\u0026amp;family=DM+Sans:ital,wght@0,300;0,400;0,500;0,600;1,400\u0026amp;family=JetBrains+Mono:wght@400;600\u0026amp;display=swap\"\u003e\u003c\/p\u003e\n\u003cstyle\u003e\n:root{--ink:#0f1a13;--ink-2:#2a3d31;--ink-3:#4e6358;--forest:#1A4731;--forest-mid:#2D6A4F;--gold:#c9a84c;--gold-lt:#e8c96a;--gold-bg:#fdf6e3;--cream:#f9f6ef;--cream-2:#f1ece0;--white:#ffffff;--border:#d6cfc0;--border-lt:#e8e2d6;--radius-sm:6px;--radius-md:12px;--radius-lg:20px;--shadow-lg:0 12px 40px rgba(0,0,0,.13);--font-head:'Syne',sans-serif;--font-body:'DM Sans',sans-serif;--font-mono:'JetBrains Mono',monospace}\n.as1447-wrap,.as1447-wrap 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16px;text-decoration:none;margin-right:8px;margin-bottom:8px}\n.as1447-footer-contact a:hover{background:var(--forest);color:var(--white)}\n.as1447-footer-legal{font-size:11.5px;color:var(--ink-3);line-height:1.7;max-width:520px}\n@media(max-width:780px){\n  .as1447-hero{padding:30px 22px}\n  .as1447-hero-card-grid{grid-template-columns:repeat(2,1fr)}\n  .as1447-hero-card-row{border-right:none;border-bottom:1px solid rgba(255,255,255,.1)}\n  .as1447-tab-body{padding:20px 16px}\n  .as1447-kv td:first-child{width:140px}\n  .as1447-footer{flex-direction:column;align-items:flex-start}\n}\n\u003c\/style\u003e\n\u003cdiv class=\"as1447-wrap\" itemscope=\"\" itemtype=\"https:\/\/schema.org\/Product\"\u003e\n\u003cdiv class=\"as1447-hero\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"as1447-eyebrow\"\u003e\n\u003cspan class=\"as1447-eyebrow-dot\"\u003e\u003c\/span\u003eAuSaMicS Life Science™ · Dehydrated Culture Media · Soil \u0026amp; Agricultural Microbiology\u003c\/div\u003e\n\u003ch1 itemprop=\"name\"\u003eJensen's Medium \u003cspan style=\"font-weight: 400; font-size: 0.6em;\"\u003e(Nitrogen-Free Broth)\u003c\/span\u003e\n\u003c\/h1\u003e\n\u003cp class=\"as1447-hero-sub\"\u003eSelective Liquid Medium for Free-Living Nitrogen-Fixing Bacteria | Cat: AS-1447\u003c\/p\u003e\n\u003cp class=\"as1447-hero-desc\"\u003eThe \u003cstrong\u003eliquid (broth) format\u003c\/strong\u003e of Jensen's nitrogen-free medium, for enrichment and detection of free-living nitrogen-fixing bacteria, particularly \u003cstrong\u003eAzotobacter\u003c\/strong\u003e species. As in the agar format, selectivity relies entirely on the absence of an added nitrogen source — only diazotrophic organisms capable of fixing atmospheric nitrogen proliferate. Ideal for enrichment culture, turbidity-based screening, and downstream plating or molecular work. Australian stock held in Epping, Melbourne — same-day dispatch with full COA documentation.\u003c\/p\u003e\n\u003cdiv class=\"as1447-badges\"\u003e\n\u003cspan class=\"as1447-badge as1447-badge-gold\"\u003eAS-1447\u003c\/span\u003e \u003cspan class=\"as1447-badge as1447-badge-white\"\u003eIn Stock Australia\u003c\/span\u003e \u003cspan class=\"as1447-badge as1447-badge-white\"\u003eSame-Day Dispatch\u003c\/span\u003e \u003cspan class=\"as1447-badge as1447-badge-white\"\u003eJensen's Formula (Broth)\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"as1447-hero-card\"\u003e\n\u003cdiv class=\"as1447-hero-card-title\"\u003eQuick Specs\u003c\/div\u003e\n\u003cdiv class=\"as1447-hero-card-grid\"\u003e\n\u003cdiv class=\"as1447-hero-card-row\"\u003e\n\u003cstrong\u003eCatalogue\u003c\/strong\u003eAS-1447\u003c\/div\u003e\n\u003cdiv class=\"as1447-hero-card-row\"\u003e\n\u003cstrong\u003eDissolution\u003c\/strong\u003e24.1 g\/L\u003c\/div\u003e\n\u003cdiv class=\"as1447-hero-card-row\"\u003e\n\u003cstrong\u003eFinal pH\u003c\/strong\u003e7.2 – 7.8\u003c\/div\u003e\n\u003cdiv class=\"as1447-hero-card-row\"\u003e\n\u003cstrong\u003eSterilisation\u003c\/strong\u003e121°C \/ 15 min\u003c\/div\u003e\n\u003cdiv class=\"as1447-hero-card-row\"\u003e\n\u003cstrong\u003eReference\u003c\/strong\u003eJensen, H.L. (1942)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"as1447-tabs\" role=\"region\"\u003e\n\u003cinput type=\"radio\" name=\"as1447-tabs\" id=\"as1447-tab-1\" class=\"as1447-tab-radio\" checked\u003e \u003cinput type=\"radio\" name=\"as1447-tabs\" id=\"as1447-tab-2\" class=\"as1447-tab-radio\"\u003e \u003cinput type=\"radio\" name=\"as1447-tabs\" id=\"as1447-tab-3\" class=\"as1447-tab-radio\"\u003e \u003cinput type=\"radio\" name=\"as1447-tabs\" id=\"as1447-tab-4\" class=\"as1447-tab-radio\"\u003e\n\u003cdiv class=\"as1447-tab-nav\" role=\"tablist\"\u003e\n\u003clabel class=\"as1447-tab-btn\" for=\"as1447-tab-1\"\u003eProduct Description\u003c\/label\u003e \u003clabel class=\"as1447-tab-btn\" for=\"as1447-tab-2\"\u003eTDS\u003c\/label\u003e \u003clabel class=\"as1447-tab-btn\" for=\"as1447-tab-3\"\u003eSDS\u003c\/label\u003e \u003clabel class=\"as1447-tab-btn\" for=\"as1447-tab-4\"\u003eCOA\u003c\/label\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"as1447-tab-body\"\u003e\n\u003cdiv class=\"as1447-tab-panel\" id=\"as1447-t1\"\u003e\n\u003cdiv class=\"as1447-section-title\"\u003eOverview\u003c\/div\u003e\n\u003cp style=\"font-size: 15px; line-height: 1.8; color: var(--ink-2); margin-bottom: 20px;\"\u003e\u003cstrong\u003eJensen's Medium Broth\u003c\/strong\u003e is the liquid form of Jensen's nitrogen-free formulation, used for enrichment, detection, and cultivation of free-living nitrogen-fixing bacteria, particularly \u003cem\u003eAzotobacter\u003c\/em\u003e species. Nitrogen-fixing organisms are free-living bacteria that grow well on a nitrogen-free medium, utilising atmospheric nitrogen gas for cell protein synthesis — cell protein that is subsequently mineralised in soil after cell death, contributing to nitrogen availability for crop plants.\u003c\/p\u003e\n\u003cp style=\"font-size: 14px; line-height: 1.8; color: var(--ink-3); margin-bottom: 28px;\"\u003eAs with the agar format (AS-1444), selectivity comes from what is \u003cem\u003eabsent\u003c\/em\u003e: because no nitrogen source is added, only organisms capable of biological nitrogen fixation grow well, while non-fixing contaminants are suppressed by nitrogen limitation. The broth format is preferred for enrichment culture prior to plating, for turbidity-based screening of multiple samples, and for liquid-phase molecular or biochemical downstream work.\u003c\/p\u003e\n\u003cdiv class=\"as1447-h3\"\u003eHistorical Development\u003c\/div\u003e\n\u003cdiv class=\"as1447-mechanism\"\u003e\n\u003cp\u003eThe formulation originates from Danish-Australian soil microbiologist \u003cstrong\u003eH.L. Jensen's\u003c\/strong\u003e 1942 paper, \u003cem\u003e\"The Azotobacter of Danish agricultural soils,\"\u003c\/em\u003e published in the \u003cem\u003eProceedings of the Linnean Society of New South Wales\u003c\/em\u003e (57, 205\\u2013212) — part of a series of studies by Jensen through the early 1940s on nitrogen-fixing and cellulose-decomposing soil bacteria. The medium remains in active use in current research: a 2023 study used Jensen's nitrogen-free medium to culture reference strain \u003cem\u003eAzotobacter vinelandii\u003c\/em\u003e (ATCC BAA-1303) for label-free imaging of diazotroph activity, underscoring the formulation's continued relevance more than eight decades after its original description.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"as1447-h3\"\u003eMode of Action\u003c\/div\u003e\n\u003cdiv class=\"as1447-mechanism\"\u003e\n\u003cp\u003eSucrose acts as the sole energy source, supporting the high metabolic cost of nitrogen fixation. Sodium molybdate supplies molybdenum, an essential cofactor of the nitrogenase enzyme complex; ferrous sulfate provides iron, also required for nitrogenase function. Dipotassium phosphate buffers the medium; sodium chloride maintains osmotic equilibrium; calcium carbonate stimulates growth and, in the broth format, settles as a fine suspension that should be resuspended by mixing before inoculation and periodically during incubation.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"as1447-h3\"\u003eBroth vs. Agar — Which Format Do I Need?\u003c\/div\u003e\n\u003ctable class=\"as1447-data\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eFeature\u003c\/th\u003e\n\u003cth\u003eAS-1447 (Broth)\u003c\/th\u003e\n\u003cth\u003eAS-1444 (Agar)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormat\u003c\/td\u003e\n\u003ctd\u003eLiquid, no agar\u003c\/td\u003e\n\u003ctd\u003eSolid, 15 g\/L agar\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTotal dissolution\u003c\/td\u003e\n\u003ctd\u003e24.1 g\/L\u003c\/td\u003e\n\u003ctd\u003e39.1 g\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBest for\u003c\/td\u003e\n\u003ctd\u003eEnrichment culture, turbidity screening, liquid-phase downstream work\u003c\/td\u003e\n\u003ctd\u003eColony isolation, morphology observation, single-colony purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTypical workflow\u003c\/td\u003e\n\u003ctd\u003eEnrich in broth first, then streak to agar for isolated colonies\u003c\/td\u003e\n\u003ctd\u003eDirect plating from soil dilutions for colony counts and isolation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"as1447-h3\"\u003eApplications\u003c\/div\u003e\n\u003ctable class=\"as1447-data\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eApplication\u003c\/th\u003e\n\u003cth\u003eNotes\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eEnrichment of Azotobacter spp. from soil\/rhizosphere\u003c\/td\u003e\n\u003ctd\u003eLiquid enrichment increases recovery of low-abundance diazotrophs prior to plating\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgricultural microbiology research\u003c\/td\u003e\n\u003ctd\u003eStudy of biological nitrogen fixation and soil nitrogen cycling\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiofertiliser development\u003c\/td\u003e\n\u003ctd\u003eLiquid-scale screening and propagation of candidate nitrogen-fixing strains\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReference-strain maintenance\u003c\/td\u003e\n\u003ctd\u003eCulturing diazotroph reference strains (e.g. \u003cem\u003eAzotobacter vinelandii\u003c\/em\u003e) for downstream imaging, physiology, or genetic studies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"as1447-callout\"\u003e\n\u003cspan class=\"as1447-callout-icon\"\u003eℹ\u003c\/span\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eIntended use:\u003c\/strong\u003e For laboratory and agricultural research use only. Not intended for therapeutic, diagnostic, or food-safety testing applications.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"as1447-tab-panel\" id=\"as1447-t2\"\u003e\n\u003cdiv class=\"as1447-section-title\"\u003eTechnical Data Sheet Summary\u003c\/div\u003e\n\u003cdiv class=\"as1447-h3\"\u003eFormula — Per Litre\u003c\/div\u003e\n\u003ctable class=\"as1447-data\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003eg\/L\u003c\/th\u003e\n\u003cth\u003eRole\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eSucrose\u003c\/td\u003e\n\u003ctd\u003e20.000\u003c\/td\u003e\n\u003ctd\u003eEnergy source\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCalcium Carbonate\u003c\/td\u003e\n\u003ctd\u003e2.000\u003c\/td\u003e\n\u003ctd\u003eGrowth stimulant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDipotassium Phosphate\u003c\/td\u003e\n\u003ctd\u003e1.000\u003c\/td\u003e\n\u003ctd\u003eBuffering, phosphorus source\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnesium Sulfate\u003c\/td\u003e\n\u003ctd\u003e0.500\u003c\/td\u003e\n\u003ctd\u003eMg²⁺ ion source\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium Chloride\u003c\/td\u003e\n\u003ctd\u003e0.500\u003c\/td\u003e\n\u003ctd\u003eOsmotic equilibrium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFerrous Sulfate\u003c\/td\u003e\n\u003ctd\u003e0.100\u003c\/td\u003e\n\u003ctd\u003eNitrogenase cofactor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium Molybdate\u003c\/td\u003e\n\u003ctd\u003e0.005\u003c\/td\u003e\n\u003ctd\u003eNitrogenase cofactor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"font-size: 12px; color: var(--ink-3); margin-bottom: 20px;\"\u003eTotal: 24.1 g\/L (no agar). Final pH 7.2–7.8 at 25°C. Due to calcium carbonate, the prepared broth forms a characteristic opalescent suspension with fine precipitate — this is expected and should be resuspended by gentle mixing before inoculation.\u003c\/p\u003e\n\u003cdiv class=\"as1447-h3\"\u003ePreparation\u003c\/div\u003e\n\u003ctable class=\"as1447-kv\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eDissolution\u003c\/td\u003e\n\u003ctd\u003eSuspend 24.1 g in 1 litre of distilled\/purified water. Heat just to boiling to dissolve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDispensing\u003c\/td\u003e\n\u003ctd\u003eDispense into flasks or tubes at the required volume before sterilisation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSterilisation\u003c\/td\u003e\n\u003ctd\u003eAutoclave at 121°C for 15 minutes.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBefore Use\u003c\/td\u003e\n\u003ctd\u003eMix well to resuspend the calcium carbonate precipitate before inoculation, and periodically during incubation to keep the suspension homogeneous.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"as1447-h3\"\u003eQuality Control (Growth Promotion Testing)\u003c\/div\u003e\n\u003ctable class=\"as1447-data\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eTest Organism\u003c\/th\u003e\n\u003cth\u003eInoculum\u003c\/th\u003e\n\u003cth\u003eExpected Result (48\\u201372 h, 28\\u201330°C)\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAzotobacter chroococcum (reference strain)\u003c\/td\u003e\n\u003ctd\u003e\\u2264 100 CFU\u003c\/td\u003e\n\u003ctd\u003eGood growth (visible turbidity) without added nitrogen source\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAzotobacter vinelandii ATCC BAA-1303\u003c\/td\u003e\n\u003ctd\u003e\\u2264 100 CFU\u003c\/td\u003e\n\u003ctd\u003eGood growth (visible turbidity); reference strain used in current diazotroph research\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEscherichia coli ATCC 25922\u003c\/td\u003e\n\u003ctd\u003e\\u2264 100 CFU\u003c\/td\u003e\n\u003ctd\u003eNo growth to markedly inhibited growth (selectivity check \\u2014 non-fixing organism)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"as1447-h3\"\u003eStorage\u003c\/div\u003e\n\u003ctable class=\"as1447-kv\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eDehydrated Powder\u003c\/td\u003e\n\u003ctd\u003eStore below 30°C, tightly closed, protected from moisture and light. Hygroscopic.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrepared Broth\u003c\/td\u003e\n\u003ctd\u003e2–8°C. Discard if contaminated or discoloured. Use freshly prepared medium where possible.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"as1447-h3\"\u003eLiterature \/ References\u003c\/div\u003e\n\u003ctable class=\"as1447-data\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003e#\u003c\/th\u003e\n\u003cth\u003eReference\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003eJensen, H.L. (1942). The Azotobacter of Danish agricultural soils. \u003cem\u003eProceedings of the Linnean Society of New South Wales\u003c\/em\u003e, 57, 205\\u2013212.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003eSubba Rao, N.S. (1977). \u003cem\u003eSoil Microorganisms and Plant Growth\u003c\/em\u003e. Oxford and IBH Publishing Co., New Delhi.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003ctd\u003eLabel-free functional analysis of root-associated microbes with dynamic quantitative oblique back-illumination microscopy. \u003cem\u003ePMC10635382\u003c\/em\u003e (2023) \\u2014 Jensen's nitrogen-free medium used with \u003cem\u003eAzotobacter vinelandii\u003c\/em\u003e ATCC BAA-1303.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"as1447-tab-panel\" id=\"as1447-t3\"\u003e\n\u003cdiv class=\"as1447-section-title\"\u003eSafety Data Sheet Summary\u003c\/div\u003e\n\u003cp style=\"font-size: 14px; line-height: 1.8; color: var(--ink-2); margin-bottom: 20px;\"\u003eFull 16-section SDS prepared in accordance with the GHS and Australian WHS Regulations (Safe Work Australia).\u003c\/p\u003e\n\u003ctable class=\"as1447-kv\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGHS Classification\u003c\/td\u003e\n\u003ctd class=\"good\"\u003eNot classified as a hazardous substance or mixture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSignal Word \/ Pictograms\u003c\/td\u003e\n\u003ctd\u003eNone required\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiosafety Note\u003c\/td\u003e\n\u003ctd\u003eMicrobiological growth medium — treat inoculated cultures as potentially containing viable soil microorganisms; handle per AS\/NZS 2243 series\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTransport\u003c\/td\u003e\n\u003ctd\u003eNot classified as Dangerous Goods (ADG, IATA, IMDG); no UN Number applicable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"as1447-callout\"\u003e\n\u003cspan class=\"as1447-callout-icon\"\u003e📋\u003c\/span\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eFull 16-section GHS-compliant SDS\u003c\/strong\u003e is available as a downloadable document — contact \u003ca href=\"mailto:support@ausamics.com\" style=\"color: var(--forest); font-weight: 600;\"\u003esupport@ausamics.com\u003c\/a\u003e quoting catalogue number AS-1447.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"as1447-tab-panel\" id=\"as1447-t4\"\u003e\n\u003cdiv class=\"as1447-section-title\"\u003eCertificate of Analysis\u003c\/div\u003e\n\u003cp style=\"font-size: 14px; line-height: 1.8; color: var(--ink-2); margin-bottom: 20px;\"\u003eEach batch of Jensen's Medium Broth (AS-1447) is released with a batch-specific Certificate of Analysis.\u003c\/p\u003e\n\u003ctable class=\"as1447-data\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eTest Parameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eDehydrated Appearance\u003c\/td\u003e\n\u003ctd\u003eOff-white, fine, free-flowing, hygroscopic powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrepared Appearance\u003c\/td\u003e\n\u003ctd\u003eCream coloured, opalescent suspension with fine precipitate (resuspends on mixing)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH (prepared, 25°C)\u003c\/td\u003e\n\u003ctd\u003e7.2 – 7.8\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProductivity Test\u003c\/td\u003e\n\u003ctd\u003eAzotobacter chroococcum \/ A. vinelandii \\u2014 good growth (visible turbidity) without added nitrogen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"as1447-callout\"\u003e\n\u003cspan class=\"as1447-callout-icon\"\u003e📄\u003c\/span\u003e\n\u003cdiv\u003e\n\u003cstrong\u003eBatch-specific COA\u003c\/strong\u003e is issued with every shipment. Contact \u003ca href=\"mailto:support@ausamics.com\" style=\"color: var(--forest); font-weight: 600;\"\u003esupport@ausamics.com\u003c\/a\u003e quoting catalogue number AS-1447.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"as1447-footer\"\u003e\n\u003cdiv class=\"as1447-footer-contact\"\u003e\n\u003ca href=\"tel:+61412520598\"\u003e📞 +61 412 520 598\u003c\/a\u003e \u003ca href=\"mailto:support@ausamics.com\"\u003e📧 Product Support\u003c\/a\u003e \u003ca href=\"https:\/\/ausamics.com.au\/pages\/request-a-quote\"\u003e💬 Request a Quote\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"as1447-footer-legal\"\u003e\n\u003cstrong\u003eFor laboratory and agricultural research use only.\u003c\/strong\u003e Not intended for therapeutic, diagnostic, or food-safety testing applications.\u003cbr\u003eAuSaMicS Pty Ltd | ABN: 56 676 640 467 | 36\/25 Trafalgar Road, Epping VIC 3076, Australia | \u003ca href=\"https:\/\/ausamics.com.au\" style=\"color: var(--forest-mid);\"\u003eausamics.com.au\u003c\/a\u003e\u003cbr\u003eSame-day dispatch (Mon–Fri) · Full documentation included · Australian domestic stock\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"AuSaMicS","offers":[{"title":"Default Title","offer_id":49111095804159,"sku":"AS-1447-500G","price":139.0,"currency_code":"AUD","in_stock":true}],"url":"https:\/\/ausamics.com.au\/products\/jensens-medium-nitrogen-free-broth-selective-liquid-medium-for-free-living-nitrogen-fixing-bacteria-cat-as-1447","provider":"AuSaMicS","version":"1.0","type":"link"}