{"product_id":"marine-agar-2216","title":"Marine Agar 2216 (Zobell Marine Agar) | AS-1289 | Solid Isolation Media","description":"\u003cstyle\u003e\n.aus-nutrient-product .aus-tab-input{position:absolute;opacity:0;pointer-events:none}\n.aus-nutrient-product .aus-tabbar{display:flex;flex-wrap:wrap;gap:8px;border-bottom:3px solid #1d6fb8;margin:0 0 30px 0}\n.aus-nutrient-product .aus-tabbar label{cursor:pointer;padding:12px 20px;border-radius:12px 12px 0 0;background:#f0f4f8;border:1px solid #d8e1e8;border-bottom:none;color:#15324b;font-weight:600;font-size:14px}\n.aus-nutrient-product .aus-panel{display:none}\n#aus-t1:checked ~ .aus-tabbar label[for=\"aus-t1\"],#aus-t2:checked ~ .aus-tabbar label[for=\"aus-t2\"],#aus-t3:checked ~ .aus-tabbar label[for=\"aus-t3\"],#aus-t4:checked ~ .aus-tabbar label[for=\"aus-t4\"]{background:#2d5a3d;color:#fff;border-color:#2d5a3d}\n#aus-t1:checked ~ .aus-panels .aus-p1,#aus-t2:checked ~ .aus-panels .aus-p2,#aus-t3:checked ~ .aus-panels .aus-p3,#aus-t4:checked ~ .aus-panels .aus-p4{display:block}\n.aus-nutrient-product .aus-tab-input:focus-visible + *{outline:none}\n.aus-nutrient-product table{width:100%;border-collapse:collapse;font-size:13px}\n.aus-nutrient-product th{background:#f6f8fb;padding:11px 12px;border-bottom:2px solid #d8e1e8;text-align:left;font-weight:bold;color:#15324b}\n.aus-nutrient-product td{padding:10px 12px;border-bottom:1px solid #e2e8f0;vertical-align:top}\n.aus-nutrient-product .aus-h3{color:#15324b;font-size:22px;margin:0 0 18px 0;padding-bottom:10px;border-bottom:3px solid #1d6fb8}\n.aus-nutrient-product .aus-box{background:#f8fafc;padding:20px;border-radius:16px;border-left:5px solid #1d6fb8;margin-bottom:20px}\n.aus-nutrient-product .aus-card{background:#fff;padding:20px;border-radius:16px;border:1px solid #d8e1e8;margin-bottom:20px}\n.aus-nutrient-product .aus-scroll{overflow-x:auto}\n.aus-nutrient-product details{background:#f8fafc;border:1px solid #d8e1e8;border-left:5px solid #1d6fb8;border-radius:12px;padding:14px 18px;margin-bottom:12px}\n.aus-nutrient-product summary{font-weight:600;color:#15324b;cursor:pointer}\n@media (max-width:860px){.aus-hero-grid,.aus-main-grid{grid-template-columns:1fr !important}.aus-guarantee{min-width:100% !important}.aus-nutrient-product .aus-tabbar label{flex:1 1 45%;text-align:center}}\n\u003c\/style\u003e\n\u003c!--\nProduct: Marine Agar 2216 (Zobell Marine Agar)\nCatalogue No.: AS-1289\nManufacturer: AuSaMicS Pty Ltd, Melbourne, Australia\nCategory: Dehydrated culture medium — non-selective marine agar\nPrimary use: Isolation, enumeration and maintenance of heterotrophic marine bacteria\n--\u003e\n\u003carticle style=\"font-family: 'Segoe UI',system-ui,-apple-system,sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\" class=\"aus-nutrient-product\" lang=\"en-AU\"\u003e\u003cheader style=\"background: linear-gradient(135deg,#f0f4f8 0%,#d9e2ec 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #d8e1e8; border-left: 8px solid #2d5a3d;\"\u003e\n\u003cdiv class=\"aus-hero-grid\" style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #2d5a3d; font-weight: bold; margin-bottom: 12px; background: #fff; padding: 6px 12px; border-radius: 20px; border: 1px solid #d8e1e8;\"\u003eAuSaMicS Life Science • Marine Microbiology Media\u003c\/div\u003e\n\u003ch2 style=\"color: #15324b; margin: 0 0 8px 0; font-size: 32px; font-weight: bold; line-height: 1.25;\"\u003eMarine Agar 2216 (Zobell Marine Agar)\u003c\/h2\u003e\n\u003cp style=\"color: #243b53; margin: 0 0 15px 0; font-size: 20px; opacity: .9;\"\u003eIsolation, enumeration and maintenance of heterotrophic marine bacteria\u003c\/p\u003e\n\u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #334e68; max-width: 650px; font-weight: 500;\"\u003e\u003cstrong\u003eMarine Agar 2216 is a non-selective medium whose salts reproduce the ionic composition of seawater, allowing marine bacteria that need sea salts to grow in the laboratory.\u003c\/strong\u003e It is based on the 2216 formulation of ZoBell (1941) and is one of the most widely used media in marine microbiology and aquaculture research. Dissolve in purified water; the sea salts are already included.\u003c\/p\u003e\n\u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n\u003cspan style=\"background: #2d5a3d; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAS-1289\u003c\/span\u003e\u003cspan style=\"background: #fff; border: 2px solid #28a745; color: #28a745; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e52.4 g\/L\u003c\/span\u003e\u003cspan style=\"background: #28a745; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003epH 7.6 ± 0.2\u003c\/span\u003e\u003cspan style=\"background: #fff; border: 2px solid #2d5a3d; color: #2d5a3d; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eNon-selective Agar\u003c\/span\u003e\u003cspan style=\"background: #c9a84c; color: #1a1a1a; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eSeawater Salt Profile\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #4a5568;\"\u003e\n\u003cspan\u003e\u003cstrong\u003eIncubation:\u003c\/strong\u003e 20–25 °C, 24–72 h\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eYield:\u003c\/strong\u003e 500 g makes about 9.5 L\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size: 13px; color: #52606d; margin: 15px 0 0 0;\"\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e ZoBell Marine Agar 2216, Marine Agar, ZMA, Marine Medium 2216.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003caside class=\"aus-guarantee\" style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 280px; border: 1px solid #d8e1e8;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003eAt a Glance\u003c\/h3\u003e\n\u003cdiv style=\"font-size: 14px; color: #4a5568; text-align: center; line-height: 1.5;\"\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eBase salts\u003c\/strong\u003e – Marine Broth 2216 composition\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eSolvent\u003c\/strong\u003e – Purified water (not seawater)\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eSterilisation\u003c\/strong\u003e – 121 °C, 15 min\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eFine precipitate\u003c\/strong\u003e – normal\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eBroth version\u003c\/strong\u003e – Marine Broth 2216 (AS-1290)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-top: 18px; padding: 15px; background: #f0f4f1; border-radius: 12px; text-align: center; border: 1px solid #c8d8cc;\"\u003e\n\u003cdiv style=\"color: #2d5a3d; font-weight: 600; font-size: 14px;\"\u003eLot-specific COA, TDS and SDS\u003c\/div\u003e\n\u003cdiv style=\"color: #4a5568; font-size: 13px; margin-top: 5px;\"\u003eAvailable for every lot\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\n\u003c\/header\u003e\u003cinput type=\"radio\" name=\"aus-tabs\" id=\"aus-t1\" class=\"aus-tab-input\" checked\u003e \u003cinput type=\"radio\" name=\"aus-tabs\" id=\"aus-t2\" class=\"aus-tab-input\"\u003e \u003cinput type=\"radio\" name=\"aus-tabs\" id=\"aus-t3\" class=\"aus-tab-input\"\u003e \u003cinput type=\"radio\" name=\"aus-tabs\" id=\"aus-t4\" class=\"aus-tab-input\"\u003e\u003cnav class=\"aus-tabbar\"\u003e\u003clabel for=\"aus-t1\"\u003eProduct Description\u003c\/label\u003e\u003clabel for=\"aus-t2\"\u003eTechnical Data\u003c\/label\u003e\u003clabel for=\"aus-t3\"\u003eQuality Control\u003c\/label\u003e\u003clabel for=\"aus-t4\"\u003eSafety \u0026amp; Storage\u003c\/label\u003e\u003c\/nav\u003e\n\u003cdiv class=\"aus-panels\"\u003e\n\u003csection class=\"aus-panel aus-p1\" id=\"description\"\u003e\n\u003cdiv class=\"aus-main-grid\" style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px;\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"aus-h3\"\u003ePrinciple of the Medium\u003c\/h3\u003e\n\u003cdiv class=\"aus-box\"\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #334e68;\"\u003eMany marine bacteria need sodium, magnesium, calcium and potassium at seawater concentrations and do not grow on ordinary laboratory media. Marine Agar 2216 supplies these major ions plus the trace ions of seawater. Peptone and yeast extract provide nitrogen, vitamins and growth factors, ferric citrate supplies iron, and agar allows colony isolation and counting.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit,minmax(200px,1fr)); gap: 15px; margin-bottom: 30px;\"\u003e\n\u003cdiv style=\"background: #f0f4f1; padding: 15px; border-radius: 12px; border-left: 4px solid #2d5a3d;\"\u003e\n\u003cstrong style=\"color: #2d5a3d;\"\u003eSeawater Ions\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eMajor and trace salts at seawater levels\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff9e6; padding: 15px; border-radius: 12px; border-left: 4px solid #c9a84c;\"\u003e\n\u003cstrong style=\"color: #c9a84c;\"\u003eLow Nutrient Load\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003e6 g\/L organics suit marine heterotrophs\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #e8f2ff; padding: 15px; border-radius: 12px; border-left: 4px solid #1565c0;\"\u003e\n\u003cstrong style=\"color: #1565c0;\"\u003eBroad Recovery\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eNon-selective: supports diverse marine genera\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eTypical Marine Bacteria Recovered\u003c\/h3\u003e\n\u003cdiv class=\"aus-scroll\" style=\"margin-bottom: 30px;\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eGenus\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eGrowth\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eNotes\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cem\u003eVibrio\u003c\/em\u003e\u003c\/td\u003e\n\u003ctd\u003eGood\u003c\/td\u003e\n\u003ctd\u003eIncludes \u003cem\u003eV. harveyi\u003c\/em\u003e, \u003cem\u003eV. alginolyticus\u003c\/em\u003e; potential pathogens also grow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eAliivibrio\u003c\/em\u003e, \u003cem\u003ePhotobacterium\u003c\/em\u003e\n\u003c\/td\u003e\n\u003ctd\u003eGood\u003c\/td\u003e\n\u003ctd\u003eSome strains are bioluminescent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003ePseudoalteromonas\u003c\/em\u003e, \u003cem\u003eAlteromonas\u003c\/em\u003e\n\u003c\/td\u003e\n\u003ctd\u003eGood\u003c\/td\u003e\n\u003ctd\u003ePigmented colonies are common\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eShewanella\u003c\/em\u003e, \u003cem\u003eMarinobacter\u003c\/em\u003e\n\u003c\/td\u003e\n\u003ctd\u003eGood\u003c\/td\u003e\n\u003ctd\u003eSome \u003cem\u003eShewanella\u003c\/em\u003e strains are pink to salmon\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMarine actinomycetes (e.g. \u003cem\u003eSalinispora\u003c\/em\u003e)\u003c\/td\u003e\n\u003ctd\u003eVariable\u003c\/td\u003e\n\u003ctd\u003eSlow-growing; extended incubation needed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eRelated Marine and Aquatic Media\u003c\/h3\u003e\n\u003cdiv class=\"aus-scroll\" style=\"margin-bottom: 10px;\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eMedium\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eType\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eBest use\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #f0f4f1;\"\u003e\n\u003ctd\u003e\u003cstrong\u003eMarine Agar 2216 (AS-1289)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eNon-selective agar\u003c\/td\u003e\n\u003ctd\u003eIsolation, counts and maintenance of marine bacteria\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/ausamics.com.au\/products\/marine-broth-2216-zobell-marine-broth\" style=\"color: #1d6fb8; font-weight: 600; text-decoration: none;\"\u003eMarine Broth 2216 (AS-1290)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eNon-selective broth\u003c\/td\u003e\n\u003ctd\u003eEnrichment and biomass of marine bacteria\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/ausamics.com.au\/collections\/culture-media\" style=\"color: #1d6fb8; font-weight: 600; text-decoration: none;\"\u003eTCBS Agar (AS-1080)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eSelective differential agar\u003c\/td\u003e\n\u003ctd\u003ePathogenic \u003cem\u003eVibrio\u003c\/em\u003e such as \u003cem\u003eV. cholerae\u003c\/em\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/ausamics.com.au\/products\/alkaline-peptone-water-apw\" style=\"color: #1d6fb8; font-weight: 600; text-decoration: none;\"\u003eAlkaline Peptone Water (AS-1005)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eEnrichment broth\u003c\/td\u003e\n\u003ctd\u003e\n\u003cem\u003eVibrio\u003c\/em\u003e enrichment before TCBS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/ausamics.com.au\/products\/r2a-agar-ph-eur-medium-s\" style=\"color: #1d6fb8; font-weight: 600; text-decoration: none;\"\u003eR2A Agar (AS-1341)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eLow-nutrient agar\u003c\/td\u003e\n\u003ctd\u003eOligotrophic freshwater bacteria\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size: 12px; color: #52606d; margin: 0 0 30px 0;\"\u003eFor pathogenic \u003cem\u003eVibrio\u003c\/em\u003e: enrich in Alkaline Peptone Water, then plate on TCBS Agar.\u003c\/p\u003e\n\u003ch3 class=\"aus-h3\"\u003eComparable Products\u003c\/h3\u003e\n\u003cdiv class=\"aus-scroll\" style=\"margin-bottom: 10px;\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eSupplier\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eProduct\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eRelationship\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #f0f4f1;\"\u003e\n\u003ctd\u003e\u003cstrong\u003eAuSaMicS\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eMarine Agar 2216 (AS-1289), 52.4 g\/L\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBD Difco\u003c\/td\u003e\n\u003ctd\u003eMarine Agar 2216 (212185), 55.1 g\/L\u003c\/td\u003e\n\u003ctd\u003eComparable published formulation; uses MgCl₂ 8.8 g and sodium sulfate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHiMedia\u003c\/td\u003e\n\u003ctd\u003eZobell Marine Agar 2216 (M384)\u003c\/td\u003e\n\u003ctd\u003eComparable published formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBD Difco \/ Sigma-Aldrich\u003c\/td\u003e\n\u003ctd\u003eMarine Broth 2216\u003c\/td\u003e\n\u003ctd\u003eSame salt composition, without agar\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size: 12px; color: #52606d; margin: 0 0 30px 0;\"\u003eBased on published formulations. Trademarks belong to their respective owners.\u003c\/p\u003e\n\u003ch3 class=\"aus-h3\"\u003eAdvantages and Limitations\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit,minmax(300px,1fr)); gap: 20px; margin-bottom: 30px;\"\u003e\n\u003cdiv style=\"background: #e8f5e8; padding: 20px; border-radius: 16px; border: 1px solid #c8e6c9;\"\u003e\n\u003ch4 style=\"color: #2e7d32; margin: 0 0 12px 0; font-size: 16px;\"\u003eAdvantages\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0; font-size: 14px; color: #2e7d32;\"\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e✓ \u003cstrong\u003eNo seawater needed\u003c\/strong\u003e: consistent salinity every batch\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e✓ \u003cstrong\u003eStandard reference medium\u003c\/strong\u003e for many marine type strains\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e✓ \u003cstrong\u003eBroad recovery\u003c\/strong\u003e of marine heterotrophs\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e✓ \u003cstrong\u003eMatching broth\u003c\/strong\u003e available (AS-1290)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff3e0; padding: 20px; border-radius: 16px; border: 1px solid #ffcc02;\"\u003e\n\u003ch4 style=\"color: #e65100; margin: 0 0 12px 0; font-size: 16px;\"\u003eLimitations\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0; font-size: 14px; color: #e65100;\"\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e▲ \u003cstrong\u003eNon-selective\u003c\/strong\u003e: dilute mixed samples\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e▲ \u003cstrong\u003eSome marine bacteria\u003c\/strong\u003e need low-nutrient or special media\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e▲ \u003cstrong\u003ePotential pathogens\u003c\/strong\u003e such as \u003cem\u003eV. vulnificus\u003c\/em\u003e can grow\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e▲ \u003cstrong\u003eFine precipitate\u003c\/strong\u003e is normal; swirl before pouring\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eFrequently Asked Questions\u003c\/h3\u003e\n\u003cdetails open=\"\"\u003e\n\u003csummary\u003eWhat is Marine Agar 2216 used for?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003eIt is used to isolate, count, purify and maintain heterotrophic marine bacteria from seawater, sediments, marine organisms and aquaculture systems. Its salts reproduce the ionic composition of seawater.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eShould Marine Agar 2216 be dissolved in seawater?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003eNo. The powder already contains the sea salts. Dissolve 52.4 g in 1 L of purified water. Using seawater would roughly double the salinity and inhibit many marine bacteria.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs a precipitate in Marine Agar 2216 normal?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003eYes. A fine precipitate of calcium and magnesium salts forms during heating and autoclaving. It is normal and does not affect performance. Swirl the medium before pouring.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat temperature should Marine Agar 2216 plates be incubated at?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003eMost marine heterotrophs grow well at 20–25 °C for 24–72 hours. Use 10–15 °C for psychrophilic bacteria, and extend incubation for slow-growing isolates.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between Marine Agar 2216 and TCBS Agar?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003eMarine Agar 2216 is non-selective and supports a broad range of marine bacteria. TCBS Agar is selective and differential, and is used to isolate pathogenic Vibrio species such as V. cholerae and V. parahaemolyticus.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #d8e1e8; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003ctable style=\"font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"width: 45%;\"\u003eCatalogue No.\u003c\/th\u003e\n\u003ctd\u003eAS-1289\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eUsage\u003c\/th\u003e\n\u003ctd\u003e52.4 g\/L in purified water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eFinal pH\u003c\/th\u003e\n\u003ctd\u003e7.6 ± 0.2 at 25 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eSterilisation\u003c\/th\u003e\n\u003ctd\u003e121 °C, 15 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eIncubation\u003c\/th\u003e\n\u003ctd\u003e20–25 °C, 24–72 h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003ePrepared medium\u003c\/th\u003e\n\u003ctd\u003eLight amber, slightly opalescent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eHS code\u003c\/th\u003e\n\u003ctd\u003e3821.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #f0f4f1; padding: 20px; border-radius: 16px; border: 1px solid #c8d8cc; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #2d5a3d; margin: 0 0 12px 0; font-size: 16px; text-align: center;\"\u003eApplications\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0; font-size: 14px; color: #2d5a3d;\"\u003e\n\u003cli style=\"padding: 6px 0;\"\u003eIsolation of bacteria from seawater and sediments\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003eAquaculture and fish microbiology\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003eHeterotrophic plate counts of marine samples\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003eMaintenance of marine culture collections\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff9e6; padding: 20px; border-radius: 16px; border: 1px solid #c9a84c; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #c9a84c; margin: 0 0 12px 0; font-size: 16px; text-align: center;\"\u003ePreparation\u003c\/h4\u003e\n\u003col style=\"list-style: none; padding: 0; margin: 0; font-size: 14px; color: #5d4037;\"\u003e\n\u003cli style=\"padding: 6px 0;\"\u003e\n\u003cstrong\u003e1.\u003c\/strong\u003e Suspend 52.4 g in 1 L of purified water. Do not use seawater.\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003e\n\u003cstrong\u003e2.\u003c\/strong\u003e Heat with frequent agitation and boil for 1 minute to dissolve.\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003e\n\u003cstrong\u003e3.\u003c\/strong\u003e Autoclave at 121 °C for 15 minutes.\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003e\n\u003cstrong\u003e4.\u003c\/strong\u003e Cool to 45–50 °C, swirl to resuspend the precipitate, and pour plates.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d8e1e8;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 12px 0; font-size: 16px; text-align: center;\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eFor laboratory microbiological testing, quality control and research use only. Not intended for direct use in food, human or veterinary therapeutic applications, diagnosis, or consumption.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"aus-panel aus-p2\" id=\"technical-data\"\u003e\n\u003ch3 class=\"aus-h3\"\u003eTypical Formula (g\/L)\u003c\/h3\u003e\n\u003cdiv class=\"aus-box\" style=\"padding: 0; overflow: hidden;\"\u003e\n\u003cdiv class=\"aus-scroll\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eIngredient\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eg\/L\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eFunction\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePeptone\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e5.0\u003c\/td\u003e\n\u003ctd\u003eNitrogen, amino acids, peptides\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eYeast extract\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e1.0\u003c\/td\u003e\n\u003ctd\u003eVitamins and growth factors\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFerric citrate\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e0.1\u003c\/td\u003e\n\u003ctd\u003eIron source\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium chloride\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e19.45\u003c\/td\u003e\n\u003ctd\u003eMain seawater salt\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnesium chloride\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e5.9\u003c\/td\u003e\n\u003ctd\u003eMagnesium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnesium sulfate\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e3.24\u003c\/td\u003e\n\u003ctd\u003eMagnesium and sulfate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCalcium chloride\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e1.8\u003c\/td\u003e\n\u003ctd\u003eCalcium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePotassium chloride\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e0.55\u003c\/td\u003e\n\u003ctd\u003ePotassium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium bicarbonate\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e0.16\u003c\/td\u003e\n\u003ctd\u003eCarbonate buffering\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePotassium bromide\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e0.08\u003c\/td\u003e\n\u003ctd\u003eTrace ion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStrontium chloride\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e0.034\u003c\/td\u003e\n\u003ctd\u003eTrace ion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBoric acid\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e0.022\u003c\/td\u003e\n\u003ctd\u003eTrace ion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDisodium phosphate\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e0.008\u003c\/td\u003e\n\u003ctd\u003ePhosphorus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium silicate\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e0.004\u003c\/td\u003e\n\u003ctd\u003eTrace ion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium fluoride\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e0.0024\u003c\/td\u003e\n\u003ctd\u003eTrace ion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAmmonium nitrate\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e0.0016\u003c\/td\u003e\n\u003ctd\u003eNitrogen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e15.0\u003c\/td\u003e\n\u003ctd\u003eSolidifying agent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTotal\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e52.4\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFinal pH 7.6 ± 0.2 at 25 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size: 12px; color: #52606d; margin: -10px 0 30px 0;\"\u003eSalt composition of Marine Broth 2216 with 15 g\/L agar. Formula may be adjusted to meet performance requirements.\u003c\/p\u003e\n\u003ch3 class=\"aus-h3\"\u003eIncubation Guide\u003c\/h3\u003e\n\u003cdiv class=\"aus-scroll aus-card\" style=\"padding: 0; overflow: hidden;\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eOrganisms\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eTemperature\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eTime\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eGeneral marine heterotrophs\u003c\/td\u003e\n\u003ctd\u003e20–25 °C\u003c\/td\u003e\n\u003ctd\u003e24–72 h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePsychrophilic marine bacteria\u003c\/td\u003e\n\u003ctd\u003e10–15 °C\u003c\/td\u003e\n\u003ctd\u003e3–7 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSlow-growing isolates\u003c\/td\u003e\n\u003ctd\u003e15–25 °C\u003c\/td\u003e\n\u003ctd\u003eUp to 14 days\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eReferences\u003c\/h3\u003e\n\u003col style=\"font-size: 13px; color: #4a5568; padding-left: 20px; margin: 0;\"\u003e\n\u003cli style=\"padding: 4px 0;\"\u003eZoBell CE. Studies on marine bacteria. I. The cultural requirements of heterotrophic aerobes. \u003cem\u003eJ Mar Res\u003c\/em\u003e. 1941;4(1):42–75.\u003c\/li\u003e\n\u003cli style=\"padding: 4px 0;\"\u003eZimbro MJ, Power DA, et al., eds. \u003cem\u003eDifco \u0026amp; BBL Manual: Manual of Microbiological Culture Media\u003c\/em\u003e. 2nd ed. BD Diagnostics; 2009. Marine Agar 2216 and Marine Broth 2216.\u003c\/li\u003e\n\u003cli style=\"padding: 4px 0;\"\u003eATCC. ATCC Medium 2: Marine Agar\/Broth 2216. American Type Culture Collection.\u003c\/li\u003e\n\u003cli style=\"padding: 4px 0;\"\u003eGontang EA, Fenical W, Jensen PR. Phylogenetic diversity of gram-positive bacteria cultured from marine sediments. \u003cem\u003eAppl Environ Microbiol\u003c\/em\u003e. 2007;73(10):3272–3282.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/section\u003e\n\u003csection class=\"aus-panel aus-p3\" id=\"quality-control\"\u003e\n\u003ch3 class=\"aus-h3\"\u003eQuality Control\u003c\/h3\u003e\n\u003cdiv class=\"aus-scroll aus-card\" style=\"padding: 0; overflow: hidden;\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eParameter\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (powder)\u003c\/td\u003e\n\u003ctd\u003eLight beige, free-flowing, homogeneous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrepared medium\u003c\/td\u003e\n\u003ctd\u003eLight amber, slightly opalescent; fine precipitate acceptable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGel strength\u003c\/td\u003e\n\u003ctd\u003eFirm, comparable with 1.5% agar gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH at 25 °C\u003c\/td\u003e\n\u003ctd\u003e7.6 ± 0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eRecommended Control Strains (25 °C, 24–72 h)\u003c\/h3\u003e\n\u003cdiv class=\"aus-scroll aus-card\" style=\"padding: 0; overflow: hidden;\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eOrganism\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eExpected growth\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eVibrio harveyi\u003c\/em\u003e ATCC 14126\u003c\/td\u003e\n\u003ctd\u003e50–100\u003c\/td\u003e\n\u003ctd\u003eGood\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eAliivibrio fischeri\u003c\/em\u003e ATCC 7744\u003c\/td\u003e\n\u003ctd\u003e50–100\u003c\/td\u003e\n\u003ctd\u003eGood\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: linear-gradient(135deg,#f0f4f1 0%,#c8d8cc 100%); padding: 20px; border-radius: 16px; border-left: 5px solid #2d5a3d;\"\u003e\n\u003cstrong style=\"color: #2d5a3d;\"\u003eBatch release:\u003c\/strong\u003e Each lot is tested for appearance, pH, gel quality and growth promotion. Results are reported on the lot Certificate of Analysis.\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"aus-panel aus-p4\" id=\"safety-storage\"\u003e\n\u003ch3 class=\"aus-h3\"\u003eSafety\u003c\/h3\u003e\n\u003cdiv class=\"aus-card\"\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #334e68;\"\u003eNot classified as hazardous under the GHS. Not Dangerous Goods for transport. Avoid breathing dust and wear gloves and eye protection when weighing. This non-selective medium can grow potential pathogens such as \u003cem\u003eVibrio parahaemolyticus\u003c\/em\u003e and \u003cem\u003eV. vulnificus\u003c\/em\u003e, so handle cultures under appropriate biosafety conditions and autoclave inoculated plates before disposal. The full SDS is available for every lot.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eStorage\u003c\/h3\u003e\n\u003cdiv class=\"aus-card\"\u003e\n\u003cp style=\"margin: 0 0 8px 0; font-size: 14px; color: #334e68;\"\u003e\u003cstrong\u003eDehydrated medium:\u003c\/strong\u003e Store at 15–25 °C in a dry place with the container tightly closed. Hygroscopic. Use before the expiry date on the label.\u003c\/p\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #334e68;\"\u003e\u003cstrong\u003ePrepared plates:\u003c\/strong\u003e Store at 2–8 °C in sealed bags, protected from light. Validate the use period in your laboratory.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003cfooter style=\"margin-top: 30px; background: #f8fafc; border: 1px solid #d8e1e8; border-radius: 16px; padding: 20px; font-size: 13px; color: #52606d;\"\u003e\u003cstrong\u003eAuSaMicS Life Science:\u003c\/strong\u003e Dehydrated culture media and laboratory reagents manufactured in Melbourne, Australia. For laboratory microbiological testing, quality control and research use only. Not intended for direct use in food, human or veterinary therapeutic applications, diagnosis, or consumption. Technical content last reviewed: September 2026.\u003c\/footer\u003e\u003cscript type=\"application\/ld+json\"\u003e\n{\n \"@context\": \"https:\/\/schema.org\",\n \"@type\": \"FAQPage\",\n \"mainEntity\": [\n  {\n   \"@type\": \"Question\",\n   \"name\": \"What is Marine Agar 2216 used for?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"It is used to isolate, count, purify and maintain heterotrophic marine bacteria from seawater, sediments, marine organisms and aquaculture systems. Its salts reproduce the ionic composition of seawater.\"\n   }\n  },\n  {\n   \"@type\": \"Question\",\n   \"name\": \"Should Marine Agar 2216 be dissolved in seawater?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"No. The powder already contains the sea salts. Dissolve 52.4 g in 1 L of purified water. Using seawater would roughly double the salinity and inhibit many marine bacteria.\"\n   }\n  },\n  {\n   \"@type\": \"Question\",\n   \"name\": \"Is a precipitate in Marine Agar 2216 normal?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"Yes. A fine precipitate of calcium and magnesium salts forms during heating and autoclaving. It is normal and does not affect performance. Swirl the medium before pouring.\"\n   }\n  },\n  {\n   \"@type\": \"Question\",\n   \"name\": \"What temperature should Marine Agar 2216 plates be incubated at?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"Most marine heterotrophs grow well at 20–25 °C for 24–72 hours. Use 10–15 °C for psychrophilic bacteria, and extend incubation for slow-growing isolates.\"\n   }\n  },\n  {\n   \"@type\": \"Question\",\n   \"name\": \"What is the difference between Marine Agar 2216 and TCBS Agar?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"Marine Agar 2216 is non-selective and supports a broad range of marine bacteria. TCBS Agar is selective and differential, and is used to isolate pathogenic Vibrio species such as V. cholerae and V. parahaemolyticus.\"\n   }\n  }\n ]\n}\n  \u003c\/script\u003e\u003c\/article\u003e","brand":"Ausamics Life Science","offers":[{"title":"500 g","offer_id":48388446748927,"sku":"AS-1289-500G","price":95.0,"currency_code":"AUD","in_stock":true},{"title":"2 kg","offer_id":48388446781695,"sku":"AS-1289-2KG","price":325.0,"currency_code":"AUD","in_stock":true}],"url":"https:\/\/ausamics.com.au\/products\/marine-agar-2216","provider":"AuSaMicS","version":"1.0","type":"link"}