{"product_id":"mueller-kauffman-tetrathionate-novobiocin-broth-base-mkttn-b","title":"MKTTn Broth Base (AS-1301) | ISO 6579 Salmonella Enrichment","description":"\u003cdiv class=\"aus-mkttn-product\" style=\"font-family: 'Segoe UI', system-ui, -apple-system, sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\"\u003e\n\u003c!-- Hero Section --\u003e\n\u003cdiv style=\"background: linear-gradient(135deg, #f8fbff 0%, #eef5fb 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #d8e1e8;\"\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #1d6fb8; font-weight: bold; margin-bottom: 12px; background: #e8f2ff; padding: 6px 12px; border-radius: 20px;\"\u003eAuSaMicS Life Science • Culture Media\u003c\/div\u003e\n\u003ch1 style=\"color: #15324b; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003eMueller Kauffmann Tetrathionate Novobiocin Broth Base\u003c\/h1\u003e\n\u003ch2 style=\"color: #243b53; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: 0.9;\"\u003eMKTTn Broth Base • Selective enrichment medium for \u003cem\u003eSalmonella\u003c\/em\u003e spp.\u003c\/h2\u003e\n\u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #334e68; max-width: 760px; font-weight: 500;\"\u003eMueller Kauffmann Tetrathionate Novobiocin Broth Base (MKTTn) is a \u003cstrong\u003eselective enrichment medium\u003c\/strong\u003e used for the recovery of \u003cstrong\u003e\u003cem\u003eSalmonella\u003c\/em\u003e spp.\u003c\/strong\u003e from foods, animal feeds, and environmental samples. When completed with iodine-iodide solution and novobiocin supplement, the medium offers strong selectivity against competing flora while allowing target \u003cem\u003eSalmonella\u003c\/em\u003e to multiply effectively during the secondary enrichment step.\u003c\/p\u003e\n\u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n\u003cspan style=\"background: #1d6fb8; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600;\"\u003eAS-1301\u003c\/span\u003e \u003cspan style=\"background: #fff; border: 2px solid #1d6fb8; color: #1d6fb8; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600;\"\u003eMKTTn Broth Base\u003c\/span\u003e \u003cspan style=\"background: #28a745; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600;\"\u003e✓ Salmonella Enrichment\u003c\/span\u003e \u003cspan style=\"background: #ef6c00; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600;\"\u003e Food \u0026amp; Environmental Testing\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #4a5568;\"\u003e\n\u003cspan\u003e\u003cstrong\u003eTarget Organism:\u003c\/strong\u003e \u003cem\u003eSalmonella\u003c\/em\u003e spp.\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eIncubation:\u003c\/strong\u003e 41.5 ± 1 °C\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eAvailable:\u003c\/strong\u003e COA + TDS + SDS\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 290px; border: 1px solid #d8e1e8;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003e Selective Enrichment Specialist\u003c\/h4\u003e\n\u003cdiv style=\"font-size: 14px; color: #4a5568; text-align: center; line-height: 1.5;\"\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eTetrathionate System\u003c\/strong\u003e - inhibits coliform competitors\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eNovobiocin\u003c\/strong\u003e - improves selectivity vs. Proteus and others\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eBrilliant Green + Ox Bile\u003c\/strong\u003e - added suppression of competing flora\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eCalcium Carbonate\u003c\/strong\u003e - buffering support\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eMethod Ready\u003c\/strong\u003e - suitable for ISO-style workflows\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-top: 20px; padding: 15px; background: #fff8e1; border-radius: 12px; text-align: center; border: 1px solid #ffe082;\"\u003e\n\u003cdiv style=\"color: #ef6c00; font-weight: 600; font-size: 14px;\"\u003eSecondary Selective Enrichment\u003c\/div\u003e\n\u003cdiv style=\"color: #4a5568; font-size: 13px; margin-top: 5px;\"\u003eTypically used in parallel with RVS-type enrichment workflows\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Main Grid --\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\"\u003e\n\u003c!-- Left Column --\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003e🔬 Product Overview\u003c\/h3\u003e\n\u003cdiv style=\"background: #f8fafc; padding: 20px; border-radius: 16px; border-left: 5px solid #1d6fb8;\"\u003e\n\u003cp style=\"margin: 0 0 15px 0;\"\u003eMKTTn Broth Base is formulated for the \u003cstrong\u003eselective enrichment of \u003cem\u003eSalmonella\u003c\/em\u003e spp.\u003c\/strong\u003e from samples containing mixed microbial populations. It is especially valuable where samples contain \u003cstrong\u003ehigh levels of competing background flora\u003c\/strong\u003e, such as meat, poultry, egg products, milk powder, feed materials, and environmental swabs.\u003c\/p\u003e\n\u003cp style=\"margin: 0 0 15px 0;\"\u003eThe complete medium is prepared by supplementing the dehydrated base with \u003cstrong\u003eiodine-potassium iodide solution\u003c\/strong\u003e to generate tetrathionate in situ, together with \u003cstrong\u003enovobiocin\u003c\/strong\u003e to strengthen selectivity. This combination enhances suppression of organisms such as \u003cem\u003eProteus\u003c\/em\u003e, \u003cem\u003ePseudomonas\u003c\/em\u003e, and \u003cem\u003eCitrobacter\u003c\/em\u003e, while supporting multiplication of target \u003cem\u003eSalmonella\u003c\/em\u003e.\u003c\/p\u003e\n\u003cp style=\"margin: 0;\"\u003eMKTTn is widely used as a \u003cstrong\u003esecond selective enrichment step\u003c\/strong\u003e following pre-enrichment, prior to plating on selective isolation agars such as XLD and other confirmatory media.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003e⚗️ Principle of the Medium\u003c\/h3\u003e\n\u003cdiv style=\"background: #f8fafc; padding: 20px; border-radius: 16px; border-left: 5px solid #1d6fb8;\"\u003e\n\u003cp style=\"margin: 0 0 15px 0;\"\u003eMKTTn uses a multi-component selectivity system to suppress competing flora while maintaining recovery of \u003cem\u003eSalmonella\u003c\/em\u003e:\u003c\/p\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; font-family: monospace; font-size: 14px; color: #4a5568; border: 1px solid #d8e1e8; text-align: center;\"\u003eSodium thiosulfate + iodine\/iodide\u003cbr\u003e↓\u003cbr\u003eTetrathionate generated in situ\u003cbr\u003e↓\u003cbr\u003eInhibition of coliform competitors\u003cbr\u003e\u003cbr\u003eBrilliant green + ox bile\u003cbr\u003e↓\u003cbr\u003eAdditional suppression of Gram-positive and selected Gram-negative flora\u003cbr\u003e\u003cbr\u003eNovobiocin\u003cbr\u003e↓\u003cbr\u003eStronger inhibition of Proteus, Pseudomonas, Citrobacter\u003c\/div\u003e\n\u003cdiv style=\"background: #fff8e1; padding: 12px; border-radius: 8px; margin-top: 15px; border: 1px solid #ffe082;\"\u003e\n\u003cstrong style=\"color: #ef6c00;\"\u003eBuffering effect:\u003c\/strong\u003e calcium carbonate helps neutralize acid produced by competing organisms and supports medium stability during enrichment.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003e🧫 Applications\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(280px, 1fr)); gap: 20px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d8e1e8; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n\u003ch4 style=\"color: #1565c0; margin: 0 0 10px 0; font-size: 16px;\"\u003e Food Testing\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eSelective enrichment of \u003cem\u003eSalmonella\u003c\/em\u003e from meat, poultry, egg products, milk powder, spices, and processed foods.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d8e1e8; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n\u003ch4 style=\"color: #1565c0; margin: 0 0 10px 0; font-size: 16px;\"\u003e Feed Microbiology\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eUseful for recovery of \u003cem\u003eSalmonella\u003c\/em\u003e from animal feed materials and related agricultural matrices.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d8e1e8; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n\u003ch4 style=\"color: #1565c0; margin: 0 0 10px 0; font-size: 16px;\"\u003e Environmental Monitoring\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eSuitable for environmental swabs and process hygiene samples where \u003cem\u003eSalmonella\u003c\/em\u003e screening is required.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d8e1e8; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n\u003ch4 style=\"color: #1565c0; margin: 0 0 10px 0; font-size: 16px;\"\u003e📋 ISO-Style Workflow Support\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eUsed as a selective enrichment step following non-selective pre-enrichment in buffered peptone water.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003e📊 Key Features\u003c\/h3\u003e\n\u003cdiv style=\"background: #e8f2ff; padding: 20px; border-radius: 16px; border-left: 5px solid #1d6fb8;\"\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(240px, 1fr)); gap: 15px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 12px; border: 1px solid #d8e1e8;\"\u003e\n\u003cstrong\u003eTetrathionate Selectivity\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #4a5568;\"\u003eGenerated in situ to suppress coliform competitors.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 12px; border: 1px solid #d8e1e8;\"\u003e\n\u003cstrong\u003eNovobiocin Enhancement\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #4a5568;\"\u003eImproves inhibition of \u003cem\u003eProteus\u003c\/em\u003e, \u003cem\u003ePseudomonas\u003c\/em\u003e, and \u003cem\u003eCitrobacter\u003c\/em\u003e.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 12px; border: 1px solid #d8e1e8;\"\u003e\n\u003cstrong\u003eBuffered Performance\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #4a5568;\"\u003eCalcium carbonate helps counter acidification by competing flora.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 14px; border-radius: 12px; border: 1px solid #d8e1e8;\"\u003e\n\u003cstrong\u003eHigh-Flora Sample Suitability\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #4a5568;\"\u003eWell suited to matrices with heavy microbial background.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003e🧪 Composition (per litre, complete medium basis)\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px; border: 1px solid #d8e1e8; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n\u003cthead style=\"background: #f6f8fb;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 14px 12px; border-bottom: 2px solid #d8e1e8; text-align: left;\"\u003eComponent\u003c\/th\u003e\n\u003cth style=\"padding: 14px 12px; border-bottom: 2px solid #d8e1e8; text-align: left;\"\u003eAmount\u003c\/th\u003e\n\u003cth style=\"padding: 14px 12px; border-bottom: 2px solid #d8e1e8; text-align: left;\"\u003eFunction\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003eMeat Peptone \/ Casein Peptone\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003e7.0 g\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eNutrient support\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003eSodium Chloride\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003e2.3 g\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eOsmotic balance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003eCalcium Carbonate\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003e25.0 g\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eBuffering agent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003eOx Bile (dried)\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003e4.75 g\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eSelective inhibition\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003eBrilliant Green\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003e0.0095 g\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eSelective dye\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003eSodium Thiosulfate·5H₂O\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003e40.7 g\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eTetrathionate precursor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003eIodine-Potassium Iodide Solution\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003e20 mL\/L\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eGenerates tetrathionate in situ\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px;\"\u003eNovobiocin Supplement\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003e40 mg\/L\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eImproves selectivity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-top: 12px; background: #fff8e1; padding: 12px; border-radius: 8px; border: 1px solid #ffe082; font-size: 13px; color: #6d4c41;\"\u003e\n\u003cstrong\u003eFinal pH:\u003c\/strong\u003e 8.2 ± 0.2 at 25 °C\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003e Preparation\u003c\/h3\u003e\n\u003cdiv style=\"background: #f8fafc; padding: 22px; border-radius: 16px; border-left: 5px solid #28a745;\"\u003e\n\u003col style=\"margin: 0; padding-left: 20px; color: #4a5568; font-size: 14px;\"\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eSuspend \u003cstrong\u003e79.7395 g\u003c\/strong\u003e of dehydrated MKTTn base in \u003cstrong\u003e1 litre\u003c\/strong\u003e purified water.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eHeat with agitation and boil for \u003cstrong\u003e5–10 minutes\u003c\/strong\u003e until completely dissolved.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003e\u003cstrong\u003eDo not autoclave.\u003c\/strong\u003e\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eCool to \u003cstrong\u003ebelow 45 °C\u003c\/strong\u003e.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eAseptically add:\n\u003cul style=\"margin-top: 8px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003e20 mL\/L\u003c\/strong\u003e iodine-potassium iodide solution\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003e40 mg\/L\u003c\/strong\u003e novobiocin supplement, filter sterilized\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eMix thoroughly and distribute into sterile tubes or bottles as required.\u003c\/li\u003e\n\u003cli\u003eUse the completed broth on the same day after supplement addition.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: linear-gradient(135deg, #fff3e0 0%, #ffe0b3 100%); padding: 20px; border-radius: 16px; border-left: 5px solid #ff9800;\"\u003e\n\u003ch4 style=\"color: #e65100; margin: 0 0 15px 0; font-size: 16px;\"\u003e💡 Typical Workflow\u003c\/h4\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(220px, 1fr)); gap: 15px;\"\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #e65100;\"\u003e1. Pre-Enrichment\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003ePre-enrich sample in buffered peptone water for 16–20 h.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #e65100;\"\u003e2. Selective Enrichment\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eTransfer 0.1 mL or 1 mL into 10 mL MKTTn.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #e65100;\"\u003e3. Incubate\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eIncubate at 41.5 ± 1 °C for 24 ± 3 h.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #e65100;\"\u003e4. Subculture\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003ePlate onto XLD and a second selective isolation agar.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Right Column --\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #d8e1e8; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003e📋 Technical Specifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb; width: 45%;\"\u003eProduct Name\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eMueller Kauffmann Tetrathionate Novobiocin Broth Base\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eShort Name\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eMKTTn Broth Base\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eCatalogue Number\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eAS-1301\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eMedium Type\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eSelective enrichment broth base\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003ePrimary Use\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eEnrichment of \u003cem\u003eSalmonella\u003c\/em\u003e spp.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eSupplement Requirement\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eIodine-iodide solution + novobiocin\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eFinal pH\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e8.2 ± 0.2 at 25 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eIncubation\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e41.5 ± 1 °C for 24 ± 3 h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003ePre-Enrichment Step\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eTypically after BPW pre-enrichment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eHS Code\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e3821.00.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff8e1; padding: 20px; border-radius: 16px; border: 1px solid #ffe082; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #ef6c00; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e🌡️ Incubation Notes\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #6d4c41;\"\u003e✓ Incubate at \u003cstrong\u003e41.5 ± 1 °C\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #6d4c41;\"\u003e✓ Typical duration: \u003cstrong\u003e24 ± 3 h\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #6d4c41;\"\u003e✓ Use after non-selective pre-enrichment\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #6d4c41;\"\u003e✓ Subculture onto selective agars after enrichment\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #e8f5e8; padding: 20px; border-radius: 16px; border: 1px solid #c8e6c9; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #2e7d32; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e✅ Selectivity Advantages\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ Good performance with high-background samples\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ Enhanced suppression of competing Enterobacteriaceae-like flora\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ Suitable as one branch of dual selective enrichment strategy\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ Supports routine food and environmental microbiology screening\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #e3f2fd; padding: 20px; border-radius: 16px; border: 1px solid #90caf9; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #1565c0; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e❄️ Storage \u0026amp; Handling\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ Base medium without supplements: \u003cstrong\u003e2–8 °C\u003c\/strong\u003e up to 1 month\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ Completed broth with iodine + novobiocin: use the \u003cstrong\u003esame day\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ Do not autoclave the finished medium\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ Prepare supplements aseptically immediately before use\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d8e1e8;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e📄 Available Documentation\u003c\/h4\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr; gap: 10px;\"\u003e\n\u003cdiv style=\"background: #f8fafc; padding: 12px; border-radius: 10px; text-align: center; border: 1px solid #d8e1e8;\"\u003eTechnical Data Sheet (TDS)\u003c\/div\u003e\n\u003cdiv style=\"background: #f8fafc; padding: 12px; border-radius: 10px; text-align: center; border: 1px solid #d8e1e8;\"\u003eCertificate of Analysis (COA)\u003c\/div\u003e\n\u003cdiv style=\"background: #f8fafc; padding: 12px; border-radius: 10px; text-align: center; border: 1px solid #d8e1e8;\"\u003eSafety Data Sheet (SDS)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Related Products --\u003e\n\u003cdiv style=\"background: #f8fafc; padding: 30px; border-radius: 18px; border: 1px solid #d8e1e8; margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 20px 0; text-align: center; font-size: 20px;\"\u003e🧬 Related Salmonella Testing Products\u003c\/h3\u003e\n\u003cdiv style=\"margin-bottom: 25px;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003eComplementary Enrichment \u0026amp; Isolation Media\u003c\/h4\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(180px, 1fr)); gap: 15px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #1d6fb8; margin: 0 0 8px 0; font-size: 14px;\"\u003eBuffered Peptone Water\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eNon-selective pre-enrichment step\u003c\/p\u003e\n\u003ca style=\"display: inline-block; background: #1d6fb8; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/pages\/request-a-quote\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #2e7d32; margin: 0 0 8px 0; font-size: 14px;\"\u003eRVS Broth\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eParallel selective enrichment\u003c\/p\u003e\n\u003ca style=\"display: inline-block; background: #2e7d32; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/pages\/request-a-quote\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #ef6c00; margin: 0 0 8px 0; font-size: 14px;\"\u003eXLD Agar\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eSelective isolation agar\u003c\/p\u003e\n\u003ca style=\"display: inline-block; background: #ef6c00; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/pages\/request-a-quote\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #d8e1e8;\"\u003e\n\u003ch5 style=\"color: #6f42c1; margin: 0 0 8px 0; font-size: 14px;\"\u003eChromogenic Salmonella Agar\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eConfirmatory isolation support\u003c\/p\u003e\n\u003ca style=\"display: inline-block; background: #6f42c1; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\" href=\"\/pages\/request-a-quote\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 18px; border-radius: 14px; border: 1px solid #d8e1e8; text-align: center;\"\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eNeed this converted into your full \u003cstrong\u003eShopify tabs format\u003c\/strong\u003e with Product Description \/ TDS \/ SDS \/ COA sections? This content is ready for that layout.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Footer --\u003e\n\u003cdiv style=\"background: #15324b; color: #fff; padding: 22px; border-radius: 16px; font-size: 13px; line-height: 1.7;\"\u003e\n\u003cdiv style=\"margin-bottom: 8px;\"\u003e\n\u003cstrong\u003eAuSaMicS Pty Ltd\u003c\/strong\u003e • 31 Longview CT, Thomastown, VIC 3074, Australia • ABN 56676640467\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 8px;\"\u003eWebsite: www.ausamics.com.au • Email: support@ausamics.com • Phone: +61 412 520 598\u003c\/div\u003e\n\u003cdiv\u003eFor food and environmental microbiology use only. Product suitability should be verified by the user under validated pre-enrichment, selective enrichment, isolation, and confirmation procedures.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Ausamics Life Science","offers":[{"title":"250 g","offer_id":48388166222079,"sku":"AS-1301-250G","price":65.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":48388166254847,"sku":"AS-1301-500G","price":95.0,"currency_code":"AUD","in_stock":true},{"title":"2.5 kg","offer_id":48388166287615,"sku":"AS-1301-2.5KG","price":445.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/MKTTn_Broth_Base_AS-1301.jpg?v=1775878218","url":"https:\/\/ausamics.com.au\/products\/mueller-kauffman-tetrathionate-novobiocin-broth-base-mkttn-b","provider":"AuSaMicS","version":"1.0","type":"link"}