{"product_id":"nitrate-broth","title":"Nitrate Broth (AS-1306) | Nitrate Reduction \u0026 Denitrification Assay","description":"\u003cdiv class=\"aus-nutrient-product\" style=\"font-family: 'Segoe UI', system-ui, -apple-system, sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\"\u003e\n\u003cdiv class=\"aus-hero-section\" style=\"background: linear-gradient(135deg, #fef2f2 0%, #fee2e2 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #fecaca; border-left: 8px solid #991b1b;\"\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\" class=\"aus-hero-grid\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #991b1b; font-weight: bold; margin-bottom: 12px; background: #fff; padding: 6px 12px; border-radius: 20px; border: 1px solid #fecaca;\"\u003eAuSaMicS Life Science • Biochemical Identification\u003c\/div\u003e\n\u003ch1 style=\"color: #7f1d1d; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003eNitrate Broth\u003c\/h1\u003e\n\u003ch2 style=\"color: #991b1b; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: 0.9;\"\u003eDefinitive Nitrate Reduction \u0026amp; Denitrification Test\u003c\/h2\u003e\n\u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #450a0a; max-width: 600px; font-weight: 500;\"\u003eThe industry standard for differentiating \u003cstrong\u003eEnterobacteriaceae\u003c\/strong\u003e and identifying \u003cstrong\u003ePseudomonas aeruginosa\u003c\/strong\u003e. Designed for the precise detection of nitrate reduction to nitrite, nitrogen gas, or ammonia.\u003c\/p\u003e\n\u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n\u003cspan style=\"background: #991b1b; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAS-1306\u003c\/span\u003e \u003cspan style=\"background: #fff; border: 2px solid #b91c1c; color: #b91c1c; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e⚗️ Redox Assay\u003c\/span\u003e \u003cspan style=\"background: #b91c1c; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e✓ ISO 21528-2\u003c\/span\u003e \u003cspan style=\"background: #475569; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🧪 Clinical Grade\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #7f1d1d;\"\u003e\n\u003cspan\u003e\u003cstrong\u003eSubstrate:\u003c\/strong\u003e Potassium Nitrate\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eIndicator:\u003c\/strong\u003e Griess Reagents\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eStandard:\u003c\/strong\u003e FDA-BAM \/ API 20E\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 280px; border: 1px solid #fecaca;\" class=\"aus-guarantee\"\u003e\n\u003ch4 style=\"color: #7f1d1d; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003eDiagnostic Utility\u003c\/h4\u003e\n\u003cdiv style=\"font-size: 14px; color: #450a0a; text-align: center; line-height: 1.5;\"\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eDual Detection\u003c\/strong\u003e - Nitrite \u0026amp; Gas (N₂)\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eHigh Sensitivity\u003c\/strong\u003e - Detects weak reducers\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eUnambiguous\u003c\/strong\u003e - Clear red color shift\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eStable\u003c\/strong\u003e - 6-month shelf life (prepared)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-top: 20px; padding: 15px; background: #fef2f2; border-radius: 12px; text-align: center; border: 1px solid #fee2e2;\"\u003e\n\u003cdiv style=\"color: #991b1b; font-weight: 600; font-size: 14px;\"\u003eKey Reaction\u003c\/div\u003e\n\u003cdiv style=\"color: #b91c1c; font-size: 13px; margin-top: 5px;\"\u003eNO₃ → NO₂ → N₂ \/ NH₃\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\" class=\"aus-main-grid\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #7f1d1d; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #991b1b;\"\u003e🔬 Interpretation Protocol\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 20px;\"\u003e\n\u003cdiv style=\"background: #fafafa; padding: 20px; border-radius: 16px; border-left: 5px solid #b91c1c;\"\u003e\n\u003ch4 style=\"color: #991b1b; margin: 0 0 10px 0; font-size: 16px;\"\u003eStep 1: Griess Reagents\u003c\/h4\u003e\n\u003cp style=\"font-size: 13px; margin: 0;\"\u003eAdd Reagents A \u0026amp; B. \u003cstrong\u003eImmediate Red\u003c\/strong\u003e = Positive for Nitrite reduction. If no color, proceed to Step 2.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fafafa; padding: 20px; border-radius: 16px; border-left: 5px solid #475569;\"\u003e\n\u003ch4 style=\"color: #1e293b; margin: 0 0 10px 0; font-size: 16px;\"\u003eStep 2: Zinc Dust Confirmation\u003c\/h4\u003e\n\u003cp style=\"font-size: 13px; margin: 0;\"\u003eAdd Zinc grains. \u003cstrong\u003eNo Color\u003c\/strong\u003e = Positive Denitrification (N₂). \u003cstrong\u003eRed Color\u003c\/strong\u003e = True Negative (Nitrate still present).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #7f1d1d; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #991b1b;\"\u003e🧪 Formulation Details\u003c\/h3\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #e2e8f0;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px;\"\u003e\n\u003cthead style=\"background: #f8fafc;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 12px; text-align: left;\"\u003eIngredient\u003c\/th\u003e\n\u003cth style=\"padding: 12px; text-align: left;\"\u003eConcentration\u003c\/th\u003e\n\u003cth style=\"padding: 12px; text-align: left;\"\u003eFunction\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #eee;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003ePeptone\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #b91c1c; font-weight: 600;\"\u003e5.0 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eNitrogen \u0026amp; Amino Acid Source\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #eee;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eMeat Extract\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #b91c1c; font-weight: 600;\"\u003e3.0 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eVitamins \u0026amp; Growth Factors\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003ePotassium Nitrate (KNO₃)\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #b91c1c; font-weight: 600;\"\u003e1.0 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eMetabolic Substrate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #fecaca; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #7f1d1d; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003e📋 Technical Specifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #eee;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #fff5f5;\"\u003eCat Number\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px;\"\u003eAS-1303\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #eee;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #fff5f5;\"\u003eFinal pH\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px;\"\u003e7.0 ± 0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #eee;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #fff5f5;\"\u003eYield\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px;\"\u003e9.0g per Liter\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #fff5f5;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px;\"\u003e2-8°C (Prepared)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #991b1b; color: #fff; padding: 20px; border-radius: 16px; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #fff; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e⚗️ Preparation Guide\u003c\/h4\u003e\n\u003col style=\"list-style: none; padding: 0; margin: 0; counter-reset: steps;\"\u003e\n\u003cli style=\"padding: 8px 0; font-size: 13px; position: relative; padding-left: 30px;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; top: 8px; background: #fff; color: #991b1b; width: 18px; height: 18px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 10px; font-weight: bold;\"\u003e1\u003c\/span\u003e Dissolve 9.0g in 1L distilled water.\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 13px; position: relative; padding-left: 30px;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; top: 8px; background: #fff; color: #991b1b; width: 18px; height: 18px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 10px; font-weight: bold;\"\u003e2\u003c\/span\u003e Dispense 5-10mL into tubes (add Durham tubes for gas).\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 13px; position: relative; padding-left: 30px;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; top: 8px; background: #fff; color: #991b1b; width: 18px; height: 18px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 10px; font-weight: bold;\"\u003e3\u003c\/span\u003e Autoclave at 121°C for 15 minutes.\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 13px; position: relative; padding-left: 30px;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; top: 8px; background: #fff; color: #991b1b; width: 18px; height: 18px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 10px; font-weight: bold;\"\u003e4\u003c\/span\u003e Incubate at 35-37°C for 18-48 hours.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Ausamics Life Science","offers":[{"title":"500 g","offer_id":48389557682431,"sku":"AS-1306-500G","price":85.0,"currency_code":"AUD","in_stock":true},{"title":"2 kg","offer_id":48389557715199,"sku":"AS-1306-2KG","price":295.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/Nitrate_Broth.jpg?v=1775960990","url":"https:\/\/ausamics.com.au\/products\/nitrate-broth","provider":"AuSaMicS","version":"1.0","type":"link"}