{"product_id":"of-basal-medium","title":"OF Basal Medium (AS-1310) | Carbohydrate-Free Hugh-Leifson Base","description":"\u003cdiv style=\"font-family: 'Segoe UI', system-ui, -apple-system, sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\" class=\"aus-nutrient-product\"\u003e\n    \u003cdiv style=\"background: linear-gradient(135deg, #f0f9ff 0%, #e0f2fe 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #bae6fd; border-left: 8px solid #0369a1;\" class=\"aus-hero-section\"\u003e\n        \u003cdiv class=\"aus-hero-grid\" style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\"\u003e\n            \u003cdiv\u003e\n                \u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #0369a1; font-weight: bold; margin-bottom: 12px; background: #fff; padding: 6px 12px; border-radius: 20px; border: 1px solid #bae6fd;\"\u003eAuSaMicS Life Science • Versatile Base Media\u003c\/div\u003e\n                \u003ch1 style=\"color: #0c4a6e; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003eOF Basal Medium\u003c\/h1\u003e\n                \u003ch2 style=\"color: #0369a1; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: 0.9;\"\u003eCarbohydrate-Free Hugh \u0026amp; Leifson Base\u003c\/h2\u003e\n                \u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #075985; max-width: 600px; font-weight: 500;\"\u003eA customizable semi-solid foundation for \u003cstrong\u003emetabolic profiling\u003c\/strong\u003e. Supplement with specific carbohydrates to differentiate Gram-negative bacilli based on their ability to oxidize or ferment diverse sugars.\u003c\/p\u003e\n                \n                \u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n                    \u003cspan style=\"background: #0369a1; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAS-1310\u003c\/span\u003e \n                    \u003cspan style=\"background: #fff; border: 2px solid #0ea5e9; color: #0ea5e9; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🧪 Modular Formula\u003c\/span\u003e \n                    \u003cspan style=\"background: #0ea5e9; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e✓ Custom Carbohydrates\u003c\/span\u003e \n                    \u003cspan style=\"background: #334155; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e⚗️ High Precision\u003c\/span\u003e\n                \u003c\/div\u003e\n                \n                \u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #0c4a6e;\"\u003e\n                    \u003cspan\u003e\u003cstrong\u003eBase:\u003c\/strong\u003e Carbohydrate-Free\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003epH Indicator:\u003c\/strong\u003e Bromothymol Blue\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eForm:\u003c\/strong\u003e Semi-Solid\u003c\/span\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n            \n            \u003cdiv class=\"aus-guarantee\" style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 280px; border: 1px solid #bae6fd;\"\u003e\n                \u003ch4 style=\"color: #0c4a6e; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003eSystem Utility\u003c\/h4\u003e\n                \u003cdiv style=\"font-size: 14px; color: #075985; text-align: center; line-height: 1.5;\"\u003e\n                    \u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eUniversal Compatibility\u003c\/strong\u003e - Supports all 1% sugar additions\u003c\/div\u003e\n                    \u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eSensitive Detection\u003c\/strong\u003e - Low peptone prevents alkaline masking\u003c\/div\u003e\n                    \u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eMotility Observation\u003c\/strong\u003e - Semi-solid agar at 0.2-0.3%\u003c\/div\u003e\n                    \u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eLong Shelf Life\u003c\/strong\u003e - 3 years dehydrated\u003c\/div\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv class=\"aus-main-grid\" style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\"\u003e\n        \u003cdiv\u003e\n            \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n                \u003ch3 style=\"color: #0c4a6e; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #0369a1;\"\u003e🔬 Research \u0026amp; Diagnostic Use\u003c\/h3\u003e\n                \u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 15px;\"\u003e\n                    \u003cdiv style=\"background: #f8fafc; padding: 20px; border-radius: 16px; border-top: 4px solid #0369a1;\"\u003e\n                        \u003ch4 style=\"color: #0c4a6e; margin: 0 0 10px 0; font-size: 16px;\"\u003eCustom Sugar Panels\u003c\/h4\u003e\n                        \u003cp style=\"font-size: 14px; margin: 0; color: #334155;\"\u003eIdeal for creating custom diagnostic panels for \u003ci\u003eVibrio\u003c\/i\u003e, \u003ci\u003eAeromonas\u003c\/i\u003e, and non-fermenting bacilli.\u003c\/p\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv style=\"background: #f8fafc; padding: 20px; border-radius: 16px; border-top: 4px solid #0369a1;\"\u003e\n                        \u003ch4 style=\"color: #0c4a6e; margin: 0 0 10px 0; font-size: 16px;\"\u003eReference Labs\u003c\/h4\u003e\n                        \u003cp style=\"font-size: 14px; margin: 0; color: #334155;\"\u003eAllows standardized basal comparison across different carbohydrate metabolic pathways.\u003c\/p\u003e\n                    \u003c\/div\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n\n            \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n                \u003ch3 style=\"color: #0c4a6e; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #0369a1;\"\u003e🧪 Nutrient Formulation (per Liter)\u003c\/h3\u003e\n                \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #e2e8f0;\"\u003e\n                    \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px;\"\u003e\n                        \u003cthead style=\"background: #f0f9ff;\"\u003e\n                            \u003ctr\u003e\n                                \u003cth style=\"padding: 12px; text-align: left;\"\u003eIngredient\u003c\/th\u003e\n                                \u003cth style=\"padding: 12px; text-align: left;\"\u003eConcentration\u003c\/th\u003e\n                                \u003cth style=\"padding: 12px; text-align: left;\"\u003eFunction\u003c\/th\u003e\n                            \u003c\/tr\u003e\n                        \u003c\/thead\u003e\n                        \u003ctbody\u003e\n                            \u003ctr style=\"border-bottom: 1px solid #f1f5f9;\"\u003e\n                                \u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eCasein Peptone\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px; color: #0369a1; font-weight: 600;\"\u003e2.0 g\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px;\"\u003eNitrogen Source (Minimized)\u003c\/td\u003e\n                            \u003c\/tr\u003e\n                            \u003ctr style=\"border-bottom: 1px solid #f1f5f9;\"\u003e\n                                \u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eSodium Chloride\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px; color: #0369a1; font-weight: 600;\"\u003e5.0 g\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px;\"\u003eOsmotic Balance\u003c\/td\u003e\n                            \u003c\/tr\u003e\n                            \u003ctr style=\"border-bottom: 1px solid #f1f5f9;\"\u003e\n                                \u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eDipotassium Phosphate\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px; color: #0369a1; font-weight: 600;\"\u003e0.3 g\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px;\"\u003epH Buffering Agent\u003c\/td\u003e\n                            \u003c\/tr\u003e\n                            \u003ctr style=\"border-bottom: 1px solid #f1f5f9;\"\u003e\n                                \u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eBromothymol Blue\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px; color: #0369a1; font-weight: 600;\"\u003e0.08 g\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px;\"\u003eVisual pH Indicator\u003c\/td\u003e\n                            \u003c\/tr\u003e\n                            \u003ctr\u003e\n                                \u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eAgar\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px; color: #0369a1; font-weight: 600;\"\u003e2.0 g\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px;\"\u003eSemi-solid Matrix\u003c\/td\u003e\n                            \u003c\/tr\u003e\n                        \u003c\/tbody\u003e\n                    \u003c\/table\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n\n        \u003cdiv\u003e\n            \u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #bae6fd; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n                \u003ch3 style=\"color: #0c4a6e; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003e📋 Technical Specs\u003c\/h3\u003e\n                \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px;\"\u003e\n                    \u003ctbody\u003e\n                        \u003ctr style=\"border-bottom: 1px solid #f1f5f9;\"\u003e\n                            \u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f0f9ff;\"\u003eCat Number\u003c\/td\u003e\n                            \u003ctd style=\"padding: 12px 8px;\"\u003eAS-1310\u003c\/td\u003e\n                        \u003c\/tr\u003e\n                        \u003ctr style=\"border-bottom: 1px solid #f1f5f9;\"\u003e\n                            \u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f0f9ff;\"\u003eFinal pH\u003c\/td\u003e\n                            \u003ctd style=\"padding: 12px 8px;\"\u003e6.8 ± 0.2\u003c\/td\u003e\n                        \u003c\/tr\u003e\n                        \u003ctr style=\"border-bottom: 1px solid #f1f5f9;\"\u003e\n                            \u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f0f9ff;\"\u003ePrepared Color\u003c\/td\u003e\n                            \u003ctd style=\"padding: 12px 8px;\"\u003eGreen\u003c\/td\u003e\n                        \u003c\/tr\u003e\n                        \u003ctr\u003e\n                            \u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f0f9ff;\"\u003eYield\u003c\/td\u003e\n                            \u003ctd style=\"padding: 12px 8px;\"\u003e9.4 g \/ L\u003c\/td\u003e\n                        \u003c\/tr\u003e\n                    \u003c\/tbody\u003e\n                \u003c\/table\u003e\n            \u003c\/div\u003e\n\n            \u003cdiv style=\"background: #0c4a6e; color: #fff; padding: 20px; border-radius: 16px; margin-bottom: 30px;\"\u003e\n                \u003ch4 style=\"color: #e0f2fe; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e⚗️ Laboratory Prep\u003c\/h4\u003e\n                \u003col style=\"list-style: none; padding: 0; margin: 0; counter-reset: steps;\"\u003e\n                    \u003cli style=\"padding: 8px 0; font-size: 13px; position: relative; padding-left: 30px;\"\u003e\n                        \u003cspan style=\"position: absolute; left: 0; top: 8px; background: #0ea5e9; color: #fff; width: 18px; height: 18px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 10px; font-weight: bold;\"\u003e1\u003c\/span\u003e Suspend 9.4g in 1L purified water.\u003c\/li\u003e\n                    \u003cli style=\"padding: 8px 0; font-size: 13px; position: relative; padding-left: 30px;\"\u003e\n                        \u003cspan style=\"position: absolute; left: 0; top: 8px; background: #0ea5e9; color: #fff; width: 18px; height: 18px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 10px; font-weight: bold;\"\u003e2\u003c\/span\u003e Heat to boiling to dissolve. Autoclave at 121°C for 15 min.\u003c\/li\u003e\n                    \u003cli style=\"padding: 8px 0; font-size: 13px; position: relative; padding-left: 30px;\"\u003e\n                        \u003cspan style=\"position: absolute; left: 0; top: 8px; background: #0ea5e9; color: #fff; width: 18px; height: 18px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 10px; font-weight: bold;\"\u003e3\u003c\/span\u003e Aseptically add 1% sterile carbohydrate solution (post-autoclave).\u003c\/li\u003e\n                    \u003cli style=\"padding: 8px 0; font-size: 13px; position: relative; padding-left: 30px;\"\u003e\n                        \u003cspan style=\"position: absolute; left: 0; top: 8px; background: #0ea5e9; color: #fff; width: 18px; height: 18px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 10px; font-weight: bold;\"\u003e4\u003c\/span\u003e Dispense into tubes; allow to solidify vertically.\u003c\/li\u003e\n                \u003c\/ol\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Ausamics Life Science","offers":[{"title":"100 g","offer_id":48389735710975,"sku":"AS-1310-100G","price":35.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":48389735743743,"sku":"AS-1310-500G","price":75.0,"currency_code":"AUD","in_stock":true},{"title":"2 kg","offer_id":48389735776511,"sku":"AS-1310-2KG","price":255.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/OF_Basal_Medium_AS-1310.jpg?v=1775976158","url":"https:\/\/ausamics.com.au\/products\/of-basal-medium","provider":"AuSaMicS","version":"1.0","type":"link"}