{"product_id":"of-glucose-medium","title":"OF Glucose Medium (Hugh \u0026 Leifson’s Oxidative-Fermentative Test)","description":"\u003cdiv style=\"font-family: 'Segoe UI', system-ui, -apple-system, sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\" class=\"aus-nutrient-product\"\u003e\n    \u003cdiv style=\"background: linear-gradient(135deg, #f0fdfa 0%, #ccfbf1 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #99f6e4; border-left: 8px solid #0d9488;\" class=\"aus-hero-section\"\u003e\n        \u003cdiv class=\"aus-hero-grid\" style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\"\u003e\n            \u003cdiv\u003e\n                \u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #0d9488; font-weight: bold; margin-bottom: 12px; background: #fff; padding: 6px 12px; border-radius: 20px; border: 1px solid #99f6e4;\"\u003eAuSaMicS Life Science • Metabolic Differentiation\u003c\/div\u003e\n                \u003ch1 style=\"color: #134e4a; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003eOF Glucose Medium\u003c\/h1\u003e\n                \u003ch2 style=\"color: #0d9488; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: 0.9;\"\u003eHugh \u0026amp; Leifson’s Oxidative-Fermentative Test\u003c\/h2\u003e\n                \u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #115e59; max-width: 600px; font-weight: 500;\"\u003eA specialized semi-solid medium used to determine the \u003cstrong\u003emetabolic pathway\u003c\/strong\u003e of glucose utilization. Essential for identifying \u003ci\u003ePseudomonas\u003c\/i\u003e, \u003ci\u003eAcinetobacter\u003c\/i\u003e, and \u003ci\u003eEnterobacteriaceae\u003c\/i\u003e.\u003c\/p\u003e\n                \n                \u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n                    \u003cspan style=\"background: #0d9488; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAS-1311\u003c\/span\u003e \n                    \u003cspan style=\"background: #fff; border: 2px solid #14b8a6; color: #14b8a6; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🧪 Dual-Tube Method\u003c\/span\u003e \n                    \u003cspan style=\"background: #14b8a6; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e✓ Clinical Microbiology\u003c\/span\u003e \n                    \u003cspan style=\"background: #0f766e; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🧬 Semi-Solid\u003c\/span\u003e\n                \u003c\/div\u003e\n                \n                \u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #134e4a;\"\u003e\n                    \u003cspan\u003e\u003cstrong\u003eIndicator:\u003c\/strong\u003e Bromothymol Blue\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eCarbohydrate:\u003c\/strong\u003e Glucose (1.0%)\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003epH:\u003c\/strong\u003e 6.8 ± 0.2\u003c\/span\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n            \n            \u003cdiv class=\"aus-guarantee\" style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 280px; border: 1px solid #99f6e4;\"\u003e\n                \u003ch4 style=\"color: #134e4a; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003eDiagnostic Indicators\u003c\/h4\u003e\n                \u003cdiv style=\"font-size: 14px; color: #115e59; text-align: center; line-height: 1.5;\"\u003e\n                    \u003cdiv style=\"margin-bottom: 12px; border-bottom: 1px solid #f0fdfa; padding-bottom: 8px;\"\u003e\n\u003cstrong\u003eYellow (Both Tubes)\u003c\/strong\u003e\u003cbr\u003eFermentative Utilization\u003c\/div\u003e\n                    \u003cdiv style=\"margin-bottom: 12px; border-bottom: 1px solid #f0fdfa; padding-bottom: 8px;\"\u003e\n\u003cstrong\u003eYellow (Open Tube Only)\u003c\/strong\u003e\u003cbr\u003eOxidative Utilization\u003c\/div\u003e\n                    \u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eGreen\/Blue\u003c\/strong\u003e\u003cbr\u003eAsaccharolytic (No utilization)\u003c\/div\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003cdiv class=\"aus-main-grid\" style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\"\u003e\n        \u003cdiv\u003e\n            \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n                \u003ch3 style=\"color: #134e4a; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #0d9488;\"\u003e🔬 Application Highlights\u003c\/h3\u003e\n                \u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 15px;\"\u003e\n                    \u003cdiv style=\"background: #f8fafc; padding: 20px; border-radius: 16px; border-left: 5px solid #0d9488;\"\u003e\n                        \u003ch4 style=\"color: #0f766e; margin: 0 0 10px 0; font-size: 16px;\"\u003eSystematic Identification\u003c\/h4\u003e\n                        \u003cp style=\"font-size: 14px; margin: 0;\"\u003eKey for separating oxidative \u003ci\u003ePseudomonas\u003c\/i\u003e from fermentative \u003ci\u003eEnterobacteriaceae\u003c\/i\u003e.\u003c\/p\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv style=\"background: #f8fafc; padding: 20px; border-radius: 16px; border-left: 5px solid #0d9488;\"\u003e\n                        \u003ch4 style=\"color: #0f766e; margin: 0 0 10px 0; font-size: 16px;\"\u003eLow-Acid Detection\u003c\/h4\u003e\n                        \u003cp style=\"font-size: 14px; margin: 0;\"\u003eHigh carbohydrate-to-peptone ratio ensures even weak acid production is detected.\u003c\/p\u003e\n                    \u003c\/div\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n\n            \u003cdiv style=\"margin-bottom: 35px;\"\u003e\n                \u003ch3 style=\"color: #134e4a; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #0d9488;\"\u003e🧪 Formula Composition\u003c\/h3\u003e\n                \u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #e2e8f0;\"\u003e\n                    \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px;\"\u003e\n                        \u003cthead style=\"background: #f0fdfa;\"\u003e\n                            \u003ctr\u003e\n                                \u003cth style=\"padding: 12px; text-align: left;\"\u003eIngredient\u003c\/th\u003e\n                                \u003cth style=\"padding: 12px; text-align: left;\"\u003eConcentration\u003c\/th\u003e\n                                \u003cth style=\"padding: 12px; text-align: left;\"\u003eFunction\u003c\/th\u003e\n                            \u003c\/tr\u003e\n                        \u003c\/thead\u003e\n                        \u003ctbody\u003e\n                            \u003ctr style=\"border-bottom: 1px solid #eee;\"\u003e\n                                \u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eCasein Peptone\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px; color: #0d9488; font-weight: 600;\"\u003e2.0 g\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px;\"\u003eNitrogen \u0026amp; Vitamins\u003c\/td\u003e\n                            \u003c\/tr\u003e\n                            \u003ctr style=\"border-bottom: 1px solid #eee;\"\u003e\n                                \u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eD-Glucose\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px; color: #0d9488; font-weight: 600;\"\u003e10.0 g\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px;\"\u003eMetabolic Substrate\u003c\/td\u003e\n                            \u003c\/tr\u003e\n                            \u003ctr style=\"border-bottom: 1px solid #eee;\"\u003e\n                                \u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eBromothymol Blue\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px; color: #0d9488; font-weight: 600;\"\u003e0.08 g\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px;\"\u003epH Indicator (Green to Yellow)\u003c\/td\u003e\n                            \u003c\/tr\u003e\n                            \u003ctr\u003e\n                                \u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eAgar\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px; color: #0d9488; font-weight: 600;\"\u003e2.0 - 3.0 g\u003c\/td\u003e\n                                \u003ctd style=\"padding: 10px;\"\u003eSemi-solidifying Agent\u003c\/td\u003e\n                            \u003c\/tr\u003e\n                        \u003c\/tbody\u003e\n                    \u003c\/table\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n\n        \u003cdiv\u003e\n            \u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #99f6e4; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n                \u003ch3 style=\"color: #134e4a; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003e📋 Technical Specs\u003c\/h3\u003e\n                \u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px;\"\u003e\n                    \u003ctbody\u003e\n                        \u003ctr style=\"border-bottom: 1px solid #f0fdfa;\"\u003e\n                            \u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f0fdfa;\"\u003eCat Number\u003c\/td\u003e\n                            \u003ctd style=\"padding: 12px 8px;\"\u003eAS-1311\u003c\/td\u003e\n                        \u003c\/tr\u003e\n                        \u003ctr style=\"border-bottom: 1px solid #f0fdfa;\"\u003e\n                            \u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f0fdfa;\"\u003eYield\u003c\/td\u003e\n                            \u003ctd style=\"padding: 12px 8px;\"\u003e20.1 g \/ L\u003c\/td\u003e\n                        \u003c\/tr\u003e\n                        \u003ctr style=\"border-bottom: 1px solid #f0fdfa;\"\u003e\n                            \u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f0fdfa;\"\u003eStorage\u003c\/td\u003e\n                            \u003ctd style=\"padding: 12px 8px;\"\u003e2 - 25°C\u003c\/td\u003e\n                        \u003c\/tr\u003e\n                        \u003ctr\u003e\n                            \u003ctd style=\"padding: 12px 8px; font-weight: 600; background: #f0fdfa;\"\u003eVisibility\u003c\/td\u003e\n                            \u003ctd style=\"padding: 12px 8px;\"\u003eForest Green (Neutral)\u003c\/td\u003e\n                        \u003c\/tr\u003e\n                    \u003c\/tbody\u003e\n                \u003c\/table\u003e\n            \u003c\/div\u003e\n\n            \u003cdiv style=\"background: #134e4a; color: #fff; padding: 20px; border-radius: 16px; margin-bottom: 30px;\"\u003e\n                \u003ch4 style=\"color: #ccfbf1; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e⚗️ Laboratory Prep\u003c\/h4\u003e\n                \u003col style=\"list-style: none; padding: 0; margin: 0; counter-reset: steps;\"\u003e\n                    \u003cli style=\"padding: 8px 0; font-size: 13px; position: relative; padding-left: 30px;\"\u003e\n                        \u003cspan style=\"position: absolute; left: 0; top: 8px; background: #14b8a6; color: #fff; width: 18px; height: 18px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 10px; font-weight: bold;\"\u003e1\u003c\/span\u003e Suspend 20.1g in 1L purified water. Mix until dissolved.\u003c\/li\u003e\n                    \u003cli style=\"padding: 8px 0; font-size: 13px; position: relative; padding-left: 30px;\"\u003e\n                        \u003cspan style=\"position: absolute; left: 0; top: 8px; background: #14b8a6; color: #fff; width: 18px; height: 18px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 10px; font-weight: bold;\"\u003e2\u003c\/span\u003e Heat to boiling to melt the agar.\u003c\/li\u003e\n                    \u003cli style=\"padding: 8px 0; font-size: 13px; position: relative; padding-left: 30px;\"\u003e\n                        \u003cspan style=\"position: absolute; left: 0; top: 8px; background: #14b8a6; color: #fff; width: 18px; height: 18px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 10px; font-weight: bold;\"\u003e3\u003c\/span\u003e Autoclave at 121°C for 15 minutes.\u003c\/li\u003e\n                    \u003cli style=\"padding: 8px 0; font-size: 13px; position: relative; padding-left: 30px;\"\u003e\n                        \u003cspan style=\"position: absolute; left: 0; top: 8px; background: #14b8a6; color: #fff; width: 18px; height: 18px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 10px; font-weight: bold;\"\u003e4\u003c\/span\u003e For testing, inoculate two tubes; overlay one with sterile paraffin oil (anaerobic).\u003c\/li\u003e\n                \u003c\/ol\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\u003c\/div\u003e","brand":"Ausamics Life Science","offers":[{"title":"500 g","offer_id":48389724537087,"sku":"AS-1311-500G","price":79.0,"currency_code":"AUD","in_stock":true},{"title":"2 kg","offer_id":48389724569855,"sku":"AS-1311-2KG","price":275.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/OF_Glucose.jpg?v=1775975627","url":"https:\/\/ausamics.com.au\/products\/of-glucose-medium","provider":"AuSaMicS","version":"1.0","type":"link"}