{"product_id":"ogye-agar-base-for-yeast-mould-counts-as-1449-ausamics","title":"OGYE Agar Base for Yeast \u0026 Mould Counts | AS-1449 AuSaMicS","description":"\u003cstyle\u003e\n.aus-nutrient-product .aus-tab-input{position:absolute;opacity:0;pointer-events:none}\n.aus-nutrient-product .aus-tabbar{display:flex;flex-wrap:wrap;gap:8px;border-bottom:3px solid #1d6fb8;margin:0 0 30px 0}\n.aus-nutrient-product .aus-tabbar label{cursor:pointer;padding:12px 20px;border-radius:12px 12px 0 0;background:#f0f4f8;border:1px solid #d8e1e8;border-bottom:none;color:#15324b;font-weight:600;font-size:14px}\n.aus-nutrient-product .aus-panel{display:none}\n#aus-t1:checked ~ .aus-tabbar label[for=\"aus-t1\"],#aus-t2:checked ~ .aus-tabbar label[for=\"aus-t2\"],#aus-t3:checked ~ .aus-tabbar label[for=\"aus-t3\"],#aus-t4:checked ~ .aus-tabbar label[for=\"aus-t4\"]{background:#2d5a3d;color:#fff;border-color:#2d5a3d}\n#aus-t1:checked ~ .aus-panels .aus-p1,#aus-t2:checked ~ .aus-panels .aus-p2,#aus-t3:checked ~ .aus-panels .aus-p3,#aus-t4:checked ~ .aus-panels .aus-p4{display:block}\n.aus-nutrient-product .aus-tab-input:focus-visible + *{outline:none}\n.aus-nutrient-product table{width:100%;border-collapse:collapse;font-size:13px}\n.aus-nutrient-product th{background:#f6f8fb;padding:11px 12px;border-bottom:2px solid #d8e1e8;text-align:left;font-weight:bold;color:#15324b}\n.aus-nutrient-product td{padding:10px 12px;border-bottom:1px solid #e2e8f0;vertical-align:top}\n.aus-nutrient-product .aus-h3{color:#15324b;font-size:22px;margin:0 0 18px 0;padding-bottom:10px;border-bottom:3px solid #1d6fb8}\n.aus-nutrient-product .aus-box{background:#f8fafc;padding:20px;border-radius:16px;border-left:5px solid #1d6fb8;margin-bottom:20px}\n.aus-nutrient-product .aus-card{background:#fff;padding:20px;border-radius:16px;border:1px solid #d8e1e8;margin-bottom:20px}\n.aus-nutrient-product .aus-scroll{overflow-x:auto}\n.aus-nutrient-product details{background:#f8fafc;border:1px solid #d8e1e8;border-left:5px solid #1d6fb8;border-radius:12px;padding:14px 18px;margin-bottom:12px}\n.aus-nutrient-product summary{font-weight:600;color:#15324b;cursor:pointer}\n@media (max-width:860px){.aus-hero-grid,.aus-main-grid{grid-template-columns:1fr !important}.aus-guarantee{min-width:100% !important}.aus-nutrient-product .aus-tabbar label{flex:1 1 45%;text-align:center}}\n\u003c\/style\u003e\n\u003c!--\nProduct: Oxytetracycline Glucose Yeast Extract Agar Base (OGYE Agar Base)\nCatalogue No.: AS-1449\nManufacturer: AuSaMicS Pty Ltd, Melbourne, Australia\nCategory: Dehydrated culture medium — selective agar for yeasts and moulds\nPrimary use: Selective enumeration of yeasts and moulds in foods\n--\u003e\n\u003carticle style=\"font-family: 'Segoe UI',system-ui,-apple-system,sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\" class=\"aus-nutrient-product\" lang=\"en-AU\"\u003e\u003cheader style=\"background: linear-gradient(135deg,#f0f4f8 0%,#d9e2ec 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #d8e1e8; border-left: 8px solid #2d5a3d;\"\u003e\n\u003cdiv class=\"aus-hero-grid\" style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #2d5a3d; font-weight: bold; margin-bottom: 12px; background: #fff; padding: 6px 12px; border-radius: 20px; border: 1px solid #d8e1e8;\"\u003eAuSaMicS Life Science • Yeast \u0026amp; Mould Media\u003c\/div\u003e\n\u003ch2 style=\"color: #15324b; margin: 0 0 8px 0; font-size: 32px; font-weight: bold; line-height: 1.25;\"\u003eOxytetra Glucose Yeast Agar Base (OGYE Agar Base)\u003c\/h2\u003e\n\u003cp style=\"color: #243b53; margin: 0 0 15px 0; font-size: 20px; opacity: .9;\"\u003eSelective enumeration of yeasts and moulds in foods (Mossel et al., 1970)\u003c\/p\u003e\n\u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #334e68; max-width: 650px; font-weight: 500;\"\u003e\u003cstrong\u003eOGYE Agar is a neutral-pH selective medium for counting yeasts and moulds in foods, made selective by adding oxytetracycline (100 mg\/L) instead of acid.\u003c\/strong\u003e Heat-stressed yeasts recover better on this medium than on acidified media, and the antibiotic suppresses bacteria, including lactobacilli.\u003c\/p\u003e\n\u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n\u003cspan style=\"background: #2d5a3d; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAS-1449\u003c\/span\u003e\u003cspan style=\"background: #fff; border: 2px solid #28a745; color: #28a745; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e37.0 g\/L\u003c\/span\u003e\u003cspan style=\"background: #28a745; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003epH 7.0 ± 0.2\u003c\/span\u003e\u003cspan style=\"background: #fff; border: 2px solid #2d5a3d; color: #2d5a3d; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eSelective Agar\u003c\/span\u003e\u003cspan style=\"background: #c9a84c; color: #1a1a1a; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eOxytetracycline 100 mg\/L\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #4a5568;\"\u003e\n\u003cspan\u003e\u003cstrong\u003eIncubation:\u003c\/strong\u003e 22–25 °C, up to 5 days\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eYield:\u003c\/strong\u003e 500 g makes about 13.5 L\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size: 13px; color: #52606d; margin: 15px 0 0 0;\"\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e OGYE Agar, Oxytetracycline-Glucose-Yeast Extract Agar, OGY Agar.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003caside class=\"aus-guarantee\" style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 280px; border: 1px solid #d8e1e8;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003eAt a Glance\u003c\/h3\u003e\n\u003cdiv style=\"font-size: 14px; color: #4a5568; text-align: center; line-height: 1.5;\"\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eSelective agent\u003c\/strong\u003e – Oxytetracycline (added)\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eBase\u003c\/strong\u003e – Yeast extract, glucose, agar\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eSterilisation\u003c\/strong\u003e – Base 121 °C, 15 min\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eNeutral pH\u003c\/strong\u003e – better recovery of stressed yeasts\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eResult type\u003c\/strong\u003e – Colony count (CFU\/g)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-top: 18px; padding: 15px; background: #f0f4f1; border-radius: 12px; text-align: center; border: 1px solid #c8d8cc;\"\u003e\n\u003cdiv style=\"color: #2d5a3d; font-weight: 600; font-size: 14px;\"\u003eLot-specific COA, TDS and SDS\u003c\/div\u003e\n\u003cdiv style=\"color: #4a5568; font-size: 13px; margin-top: 5px;\"\u003eAvailable for every lot\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\n\u003c\/header\u003e\u003cinput type=\"radio\" name=\"aus-tabs\" id=\"aus-t1\" class=\"aus-tab-input\" checked\u003e \u003cinput type=\"radio\" name=\"aus-tabs\" id=\"aus-t2\" class=\"aus-tab-input\"\u003e \u003cinput type=\"radio\" name=\"aus-tabs\" id=\"aus-t3\" class=\"aus-tab-input\"\u003e \u003cinput type=\"radio\" name=\"aus-tabs\" id=\"aus-t4\" class=\"aus-tab-input\"\u003e\u003cnav class=\"aus-tabbar\"\u003e\u003clabel for=\"aus-t1\"\u003eProduct Description\u003c\/label\u003e\u003clabel for=\"aus-t2\"\u003eTechnical Data\u003c\/label\u003e\u003clabel for=\"aus-t3\"\u003eQuality Control\u003c\/label\u003e\u003clabel for=\"aus-t4\"\u003eSafety \u0026amp; Storage\u003c\/label\u003e\u003c\/nav\u003e\n\u003cdiv class=\"aus-panels\"\u003e\n\u003csection class=\"aus-panel aus-p1\" id=\"description\"\u003e\n\u003cdiv class=\"aus-main-grid\" style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px;\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"aus-h3\"\u003ePrinciple of the Medium\u003c\/h3\u003e\n\u003cdiv class=\"aus-box\"\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #334e68;\"\u003eYeast extract supplies vitamins and nitrogen, and glucose is the energy source for fungal growth. Oxytetracycline, a broad-spectrum antibiotic, inhibits most bacteria. Because selectivity comes from the antibiotic rather than low pH, yeasts that are injured by heat or processing are counted more completely than on acidified media.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit,minmax(200px,1fr)); gap: 15px; margin-bottom: 30px;\"\u003e\n\u003cdiv style=\"background: #f0f4f1; padding: 15px; border-radius: 12px; border-left: 4px solid #2d5a3d;\"\u003e\n\u003cstrong style=\"color: #2d5a3d;\"\u003eNeutral pH\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eStressed yeast cells recover better than at low pH\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff9e6; padding: 15px; border-radius: 12px; border-left: 4px solid #c9a84c;\"\u003e\n\u003cstrong style=\"color: #c9a84c;\"\u003eAntibiotic Selectivity\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eOxytetracycline suppresses bacteria, including lactobacilli\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #e8f2ff; padding: 15px; border-radius: 12px; border-left: 4px solid #1565c0;\"\u003e\n\u003cstrong style=\"color: #1565c0;\"\u003eSimple Base\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eThree ingredients; clear medium for easy counting\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eChoosing a Yeast and Mould Medium\u003c\/h3\u003e\n\u003cdiv class=\"aus-scroll\" style=\"margin-bottom: 30px;\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eMedium\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eSelective system\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eBest suited to\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #f0f4f1;\"\u003e\n\u003ctd\u003e\u003cstrong\u003eOGYE Agar (AS-1449)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOxytetracycline, neutral pH\u003c\/td\u003e\n\u003ctd\u003eMost foods, dairy, heat-processed products\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOGGY Agar\u003c\/td\u003e\n\u003ctd\u003eOxytetracycline + gentamicin\u003c\/td\u003e\n\u003ctd\u003eProteinaceous foods with heavy Gram-negative load\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDRBC Agar\u003c\/td\u003e\n\u003ctd\u003eChloramphenicol, dichloran, rose bengal\u003c\/td\u003e\n\u003ctd\u003eFoods with water activity above 0.95; restricts mould spreading\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDG18 Agar\u003c\/td\u003e\n\u003ctd\u003eChloramphenicol, dichloran, glycerol\u003c\/td\u003e\n\u003ctd\u003eDry foods with water activity of 0.95 or below\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAcidified Potato Dextrose Agar\u003c\/td\u003e\n\u003ctd\u003eLow pH (about 3.5)\u003c\/td\u003e\n\u003ctd\u003eTraditional method; may under-count stressed yeasts\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eComparable Products\u003c\/h3\u003e\n\u003cdiv class=\"aus-scroll\" style=\"margin-bottom: 10px;\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eSupplier\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eProduct\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eRelationship\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #f0f4f1;\"\u003e\n\u003ctd\u003e\u003cstrong\u003eAuSaMicS\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eOGYE Agar Base (AS-1449)\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOxoid (Thermo Fisher)\u003c\/td\u003e\n\u003ctd\u003eOGYE Agar Base (CM0545)\u003c\/td\u003e\n\u003ctd\u003eSame published formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMerck (Millipore)\u003c\/td\u003e\n\u003ctd\u003eOGYE Agar (1.05978)\u003c\/td\u003e\n\u003ctd\u003eSame published formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHiMedia\u003c\/td\u003e\n\u003ctd\u003eOxytetra Glucose Yeast Agar Base (M639)\u003c\/td\u003e\n\u003ctd\u003eSame published formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size: 12px; color: #52606d; margin: 0 0 30px 0;\"\u003eBased on published formulations. Trademarks belong to their respective owners.\u003c\/p\u003e\n\u003ch3 class=\"aus-h3\"\u003eAdvantages and Limitations\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit,minmax(300px,1fr)); gap: 20px; margin-bottom: 30px;\"\u003e\n\u003cdiv style=\"background: #e8f5e8; padding: 20px; border-radius: 16px; border: 1px solid #c8e6c9;\"\u003e\n\u003ch4 style=\"color: #2e7d32; margin: 0 0 12px 0; font-size: 16px;\"\u003eAdvantages\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0; font-size: 14px; color: #2e7d32;\"\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e✓ \u003cstrong\u003eHigher yeast counts\u003c\/strong\u003e than acidified media for stressed cells\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e✓ \u003cstrong\u003eInhibits lactobacilli\u003c\/strong\u003e, which grow on acidified media\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e✓ \u003cstrong\u003eWell-established method\u003c\/strong\u003e, in use since 1970\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e✓ \u003cstrong\u003eClear medium\u003c\/strong\u003e for accurate counting\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff3e0; padding: 20px; border-radius: 16px; border: 1px solid #ffcc02;\"\u003e\n\u003ch4 style=\"color: #e65100; margin: 0 0 12px 0; font-size: 16px;\"\u003eLimitations\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0; font-size: 14px; color: #e65100;\"\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e▲ \u003cstrong\u003eProtein-rich foods\u003c\/strong\u003e can inactivate oxytetracycline\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e▲ \u003cstrong\u003eIncubation above 25 °C\u003c\/strong\u003e or beyond 5 days allows bacterial breakthrough\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e▲ \u003cstrong\u003eInoculum limit:\u003c\/strong\u003e no more than 1 mL of a 10⁻¹ dilution per plate\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e▲ \u003cstrong\u003eNo inhibitor of mould spreading\u003c\/strong\u003e; count early if moulds spread\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eFrequently Asked Questions\u003c\/h3\u003e\n\u003cdetails open=\"\"\u003e\n\u003csummary\u003eWhat is OGYE agar used for?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003eOGYE agar is used to count yeasts and moulds in foods. Oxytetracycline suppresses bacteria, while the neutral pH allows heat-stressed yeasts to recover.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy does OGYE use oxytetracycline instead of a low pH?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003eMossel and colleagues found that stressed yeast cells give higher counts on a neutral medium with a broad-spectrum antibiotic than on an acidified medium. Oxytetracycline also inhibits lactobacilli, which can grow on acidified media.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow much oxytetracycline is added?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003e100 mg per litre, added aseptically to the cooled base after autoclaving. Oxytetracycline is heat-sensitive and must not be autoclaved.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat temperature and time should I use?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003eIncubate aerobically at 22–25 °C for up to 5 days, examining after 3 days. Higher temperatures inactivate oxytetracycline faster and allow bacteria to break through.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhen should I use a different medium?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003eFor very proteinaceous foods or samples heavily contaminated with Gram-negative bacteria, add gentamicin (OGGY) or use DRBC agar. For low water activity foods, use DG18 agar.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #d8e1e8; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003ctable style=\"font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"width: 45%;\"\u003eCatalogue No.\u003c\/th\u003e\n\u003ctd\u003eAS-1449\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eUsage\u003c\/th\u003e\n\u003ctd\u003e37.0 g\/L (18.5 g per 500 mL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eFinal pH\u003c\/th\u003e\n\u003ctd\u003e7.0 ± 0.2 at 25 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eSupplement\u003c\/th\u003e\n\u003ctd\u003eOxytetracycline 100 mg\/L (separate)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eSterilisation\u003c\/th\u003e\n\u003ctd\u003e121 °C, 15 min (base only)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eIncubation\u003c\/th\u003e\n\u003ctd\u003e22–25 °C, 3–5 days, aerobic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003ePrepared medium\u003c\/th\u003e\n\u003ctd\u003eLight straw, clear to slightly opalescent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #f0f4f1; padding: 20px; border-radius: 16px; border: 1px solid #c8d8cc; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #2d5a3d; margin: 0 0 12px 0; font-size: 16px; text-align: center;\"\u003eApplications\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0; font-size: 14px; color: #2d5a3d;\"\u003e\n\u003cli style=\"padding: 6px 0;\"\u003eYeast and mould counts in foods and ingredients\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003eMilk and dairy products\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003eBeverages, fruit products and bakery ingredients\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003eQuality control and shelf-life studies\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff9e6; padding: 20px; border-radius: 16px; border: 1px solid #c9a84c; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #c9a84c; margin: 0 0 12px 0; font-size: 16px; text-align: center;\"\u003ePreparation\u003c\/h4\u003e\n\u003col style=\"list-style: none; padding: 0; margin: 0; font-size: 14px; color: #5d4037;\"\u003e\n\u003cli style=\"padding: 6px 0;\"\u003e\n\u003cstrong\u003e1.\u003c\/strong\u003e Suspend 18.5 g in 500 mL of purified water.\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003e\n\u003cstrong\u003e2.\u003c\/strong\u003e Heat to boiling to dissolve completely.\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003e\n\u003cstrong\u003e3.\u003c\/strong\u003e Autoclave at 121 °C for 15 minutes.\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003e\n\u003cstrong\u003e4.\u003c\/strong\u003e Cool to 45–50 °C and add oxytetracycline aseptically to 100 mg\/L (50 mg per 500 mL).\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003e\n\u003cstrong\u003e5.\u003c\/strong\u003e Mix gently and pour plates.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d8e1e8;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 12px 0; font-size: 16px; text-align: center;\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eFor laboratory microbiological testing, quality control and research use only. Not intended for direct use in food, human or veterinary therapeutic applications, diagnosis, or consumption.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"aus-panel aus-p2\" id=\"technical-data\"\u003e\n\u003ch3 class=\"aus-h3\"\u003eTypical Formula (g\/L)\u003c\/h3\u003e\n\u003cdiv class=\"aus-box\" style=\"padding: 0; overflow: hidden;\"\u003e\n\u003cdiv class=\"aus-scroll\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eIngredient\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eg\/L\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eFunction\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eYeast extract\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e5.0\u003c\/td\u003e\n\u003ctd\u003eVitamins, nitrogen and growth factors\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGlucose\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e20.0\u003c\/td\u003e\n\u003ctd\u003eEnergy and carbon source\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAgar\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e12.0\u003c\/td\u003e\n\u003ctd\u003eSolidifying agent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTotal\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e37.0\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFinal pH 7.0 ± 0.2 at 25 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOxytetracycline (added after autoclaving)\u003c\/td\u003e\n\u003ctd style=\"color: #b45309; font-weight: 600;\"\u003e0.1\u003c\/td\u003e\n\u003ctd\u003eSelective agent (100 mg\/L)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size: 12px; color: #52606d; margin: -10px 0 30px 0;\"\u003eFormula may be adjusted to meet performance requirements.\u003c\/p\u003e\n\u003ch3 class=\"aus-h3\"\u003eTest Procedure\u003c\/h3\u003e\n\u003cdiv class=\"aus-card\"\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 14px; color: #334e68;\"\u003e\u003cstrong\u003ePlating:\u003c\/strong\u003e Use pour plates or spread plates. Add no more than 1 mL of a 10⁻¹ food dilution per plate, so the food does not overwhelm the antibiotic.\u003c\/p\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 14px; color: #334e68;\"\u003e\u003cstrong\u003eIncubation:\u003c\/strong\u003e Aerobically at 22–25 °C for up to 5 days. Examine after 3 days, and count earlier if moulds spread.\u003c\/p\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #334e68;\"\u003e\u003cstrong\u003eCounting:\u003c\/strong\u003e Count yeast and mould colonies separately if required, and report as CFU per g or mL.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eRelated Standards (for user reference)\u003c\/h3\u003e\n\u003cdiv class=\"aus-card\"\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #334e68;\"\u003eISO 6611 | IDF 94 (milk and milk products) describes a yeast extract–glucose–oxytetracycline agar. ISO 21527-1 and ISO 21527-2 (foods) specify DRBC and DG18 agar.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eReferences\u003c\/h3\u003e\n\u003col style=\"font-size: 13px; color: #4a5568; padding-left: 20px; margin: 0;\"\u003e\n\u003cli style=\"padding: 4px 0;\"\u003eMossel DAA, Kleynen-Semmeling AMC, Vincentie HM, Beerens H, Catsaras M. Oxytetracycline-glucose-yeast extract agar for selective enumeration of moulds and yeasts in foods and clinical material. \u003cem\u003eJ Appl Bacteriol\u003c\/em\u003e. 1970;33:454–457.\u003c\/li\u003e\n\u003cli style=\"padding: 4px 0;\"\u003eMossel DAA, Visser M, Mengerink WHJ. A comparison of media for the enumeration of moulds and yeasts in foods and beverages. \u003cem\u003eLab Pract\u003c\/em\u003e. 1962;11:109–112.\u003c\/li\u003e\n\u003cli style=\"padding: 4px 0;\"\u003ePitt JI, Hocking AD. \u003cem\u003eFungi and Food Spoilage\u003c\/em\u003e. 3rd ed. Springer; 2009.\u003c\/li\u003e\n\u003cli style=\"padding: 4px 0;\"\u003eSalfinger Y, Tortorello ML, eds. \u003cem\u003eCompendium of Methods for the Microbiological Examination of Foods\u003c\/em\u003e. 5th ed. APHA; 2015.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/section\u003e\n\u003csection class=\"aus-panel aus-p3\" id=\"quality-control\"\u003e\n\u003ch3 class=\"aus-h3\"\u003eQuality Control\u003c\/h3\u003e\n\u003cdiv class=\"aus-scroll aus-card\" style=\"padding: 0; overflow: hidden;\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eParameter\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (powder)\u003c\/td\u003e\n\u003ctd\u003eLight beige, free-flowing, homogeneous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrepared medium\u003c\/td\u003e\n\u003ctd\u003eLight straw, clear to slightly opalescent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGel strength\u003c\/td\u003e\n\u003ctd\u003eFirm, comparable with 1.2% agar gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH at 25 °C\u003c\/td\u003e\n\u003ctd\u003e7.0 ± 0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eRecommended Control Strains (with oxytetracycline, 25 °C, 3–5 days)\u003c\/h3\u003e\n\u003cdiv class=\"aus-scroll aus-card\" style=\"padding: 0; overflow: hidden;\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eOrganism\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eExpected growth\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eColony appearance\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eSaccharomyces cerevisiae\u003c\/em\u003e ATCC 9763 (WDCM 00058)\u003c\/td\u003e\n\u003ctd\u003e50–100\u003c\/td\u003e\n\u003ctd\u003eGood growth\u003c\/td\u003e\n\u003ctd\u003eCream to white colonies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eCandida albicans\u003c\/em\u003e ATCC 10231 (WDCM 00054)\u003c\/td\u003e\n\u003ctd\u003e50–100\u003c\/td\u003e\n\u003ctd\u003eGood growth\u003c\/td\u003e\n\u003ctd\u003eCream colonies\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eAspergillus brasiliensis\u003c\/em\u003e ATCC 16404 (WDCM 00053)\u003c\/td\u003e\n\u003ctd\u003e50–100\u003c\/td\u003e\n\u003ctd\u003eGood growth\u003c\/td\u003e\n\u003ctd\u003eWhite mycelium, black spores\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eEscherichia coli\u003c\/em\u003e ATCC 25922 (WDCM 00013)\u003c\/td\u003e\n\u003ctd\u003e≥ 10³\u003c\/td\u003e\n\u003ctd\u003eInhibited\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eBacillus subtilis\u003c\/em\u003e ATCC 6633\u003c\/td\u003e\n\u003ctd\u003e≥ 10³\u003c\/td\u003e\n\u003ctd\u003eInhibited\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: linear-gradient(135deg,#f0f4f1 0%,#c8d8cc 100%); padding: 20px; border-radius: 16px; border-left: 5px solid #2d5a3d;\"\u003e\n\u003cstrong style=\"color: #2d5a3d;\"\u003eBatch release:\u003c\/strong\u003e Each lot is tested for appearance, pH, gel quality and growth promotion. Results are reported on the lot Certificate of Analysis.\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"aus-panel aus-p4\" id=\"safety-storage\"\u003e\n\u003ch3 class=\"aus-h3\"\u003eSafety\u003c\/h3\u003e\n\u003cdiv class=\"aus-card\"\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #334e68;\"\u003eThe base is not classified as hazardous under the GHS and is not Dangerous Goods for transport. Oxytetracycline is a separate product with its own SDS. Treat inoculated plates as potentially infectious and autoclave before disposal. The full SDS is available for every lot.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eStorage\u003c\/h3\u003e\n\u003cdiv class=\"aus-card\"\u003e\n\u003cp style=\"margin: 0 0 8px 0; font-size: 14px; color: #334e68;\"\u003e\u003cstrong\u003eDehydrated base:\u003c\/strong\u003e Store at 15–25 °C in a dry place with the container tightly closed. The powder is hygroscopic. Use before the expiry date on the label.\u003c\/p\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #334e68;\"\u003e\u003cstrong\u003ePrepared plates (with oxytetracycline):\u003c\/strong\u003e Store at 2–8 °C, protected from light. Validate the use period in your laboratory.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003cfooter style=\"margin-top: 30px; background: #f8fafc; border: 1px solid #d8e1e8; border-radius: 16px; padding: 20px; font-size: 13px; color: #52606d;\"\u003e\u003cstrong\u003eAuSaMicS Life Science:\u003c\/strong\u003e Dehydrated culture media and laboratory reagents manufactured in Melbourne, Australia. For laboratory microbiological testing, quality control and research use only. Not intended for direct use in food, human or veterinary therapeutic applications, diagnosis, or consumption. Technical content last reviewed: September 2026.\u003c\/footer\u003e\u003cscript type=\"application\/ld+json\"\u003e\n{\n \"@context\": \"https:\/\/schema.org\",\n \"@type\": \"FAQPage\",\n \"mainEntity\": [\n  {\n   \"@type\": \"Question\",\n   \"name\": \"What is OGYE agar used for?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"OGYE agar is used to count yeasts and moulds in foods. Oxytetracycline suppresses bacteria, while the neutral pH allows heat-stressed yeasts to recover.\"\n   }\n  },\n  {\n   \"@type\": \"Question\",\n   \"name\": \"Why does OGYE use oxytetracycline instead of a low pH?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"Mossel and colleagues found that stressed yeast cells give higher counts on a neutral medium with a broad-spectrum antibiotic than on an acidified medium. Oxytetracycline also inhibits lactobacilli, which can grow on acidified media.\"\n   }\n  },\n  {\n   \"@type\": \"Question\",\n   \"name\": \"How much oxytetracycline is added?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"100 mg per litre, added aseptically to the cooled base after autoclaving. Oxytetracycline is heat-sensitive and must not be autoclaved.\"\n   }\n  },\n  {\n   \"@type\": \"Question\",\n   \"name\": \"What temperature and time should I use?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"Incubate aerobically at 22–25 °C for up to 5 days, examining after 3 days. Higher temperatures inactivate oxytetracycline faster and allow bacteria to break through.\"\n   }\n  },\n  {\n   \"@type\": \"Question\",\n   \"name\": \"When should I use a different medium?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"For very proteinaceous foods or samples heavily contaminated with Gram-negative bacteria, add gentamicin (OGGY) or use DRBC agar. For low water activity foods, use DG18 agar.\"\n   }\n  }\n ]\n}\n  \u003c\/script\u003e\u003c\/article\u003e","brand":"AuSaMicS","offers":[{"title":"500 g","offer_id":50549408137471,"sku":"AS-1449-500G","price":89.0,"currency_code":"AUD","in_stock":true},{"title":"2 kg","offer_id":50549408170239,"sku":"AS-1449-2KG","price":325.0,"currency_code":"AUD","in_stock":true}],"url":"https:\/\/ausamics.com.au\/products\/ogye-agar-base-for-yeast-mould-counts-as-1449-ausamics","provider":"AuSaMicS","version":"1.0","type":"link"}