{"product_id":"ponceau-4r-e124-food-grade-red-colour-powder","title":"Ponceau 4R (E124) – Food Grade Red Colour Powder | CI 16255, CAS 2611-82-7 | ASD-641","description":"\u003c!--\nProduct: Ponceau 4R (E 124), C.I. 16255, CAS 2611-82-7\nCatalogue No: ASD-641\nManufacturer: Ausamics Pty Ltd, Melbourne, Australia\nCategory: Biological stains and dyes\nPrimary use: E 124 reference colour, spectrophotometric standard, model azo dye for analytical research\n--\u003e\n\u003cscript type=\"application\/ld+json\"\u003e\n{\"@context\": \"https:\/\/schema.org\", \"@type\": \"Product\", \"name\": \"Ponceau 4R (E 124), C.I. 16255\", \"sku\": \"ASD-641\", \"mpn\": \"ASD-641\", \"category\": \"Biological Stains and Dyes\", \"alternateName\": [\"Ponceau 4R\", \"E 124\", \"Acid Red 18\", \"Cochineal Red A\", \"New Coccine\", \"CI Food Red 7\", \"C.I. 16255\"], \"description\": \"Ponceau 4R (CAS 2611-82-7, C.I. 16255), water-soluble red azo dye with minimum 80 % pure dye content. Used as an E 124 reference colour, spectrophotometric standard and model azo dye in analytical and environmental research.\", \"brand\": {\"@type\": \"Brand\", \"name\": \"Ausamics\"}, \"manufacturer\": {\"@type\": \"Organization\", \"name\": \"Ausamics Pty Ltd\", \"url\": \"https:\/\/www.ausamics.com.au\"}, \"additionalProperty\": [{\"@type\": \"PropertyValue\", \"name\": \"CAS number\", \"value\": \"2611-82-7\"}, {\"@type\": \"PropertyValue\", \"name\": \"Colour Index number\", \"value\": \"16255\"}, {\"@type\": \"PropertyValue\", \"name\": \"EC number\", \"value\": \"220-036-2\"}, {\"@type\": \"PropertyValue\", \"name\": \"Molecular formula\", \"value\": \"C20H11N2Na3O10S3\"}, {\"@type\": \"PropertyValue\", \"name\": \"Molecular weight\", \"value\": \"604.47 g\/mol\"}, {\"@type\": \"PropertyValue\", \"name\": \"Dye content\", \"value\": \"Min. 80 %\"}, {\"@type\": \"PropertyValue\", \"name\": \"Absorption maximum\", \"value\": \"approx. 505 nm (water)\"}]}\n\u003c\/script\u003e\n\u003cscript type=\"application\/ld+json\"\u003e\n{\"@context\": \"https:\/\/schema.org\", \"@type\": \"FAQPage\", \"mainEntity\": [{\"@type\": \"Question\", \"name\": \"Is Ponceau 4R the same as Ponceau S?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"No. Ponceau S (C.I. 27195) is the red dye used to stain proteins on western blot membranes. Ponceau 4R (C.I. 16255) is a different azo dye, mainly used as the food colour E 124 and as an analytical reference. Do not substitute one for the other.\"}}, {\"@type\": \"Question\", \"name\": \"Is Ponceau 4R the same as Ponceau SX or FD\u0026C Red No. 4?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"No. Ponceau SX is C.I. 14700 and is sold in the US as FD\u0026C Red No. 4. Ponceau 4R is C.I. 16255. The two dyes have different structures and regulatory status.\"}}, {\"@type\": \"Question\", \"name\": \"Is Ponceau 4R permitted as a food colour?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"It is permitted in the EU as E 124 and is listed in the Australia New Zealand Food Standards Code as colour 124. It is not permitted as a food colour in the United States.\"}}, {\"@type\": \"Question\", \"name\": \"How do I make a stock solution?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Dissolve 1.0 g in about 80 mL of purified water, stir until clear and make up to 100 mL to give a 1 % w\/v solution. Store away from light.\"}}, {\"@type\": \"Question\", \"name\": \"What wavelength should I use for absorbance measurements?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Ponceau 4R absorbs most strongly at about 505 nm in water. Use this wavelength for calibration curves and dye-content checks.\"}}]}\n\u003c\/script\u003e\n\u003cstyle\u003e\n.aus-nutrient-product{font-family:\"Segoe UI\",system-ui,-apple-system,Arial,sans-serif;color:#1d2935;max-width:1200px;margin:0 auto;line-height:1.6;font-size:15px}\n.aus-nutrient-product *{box-sizing:border-box}\n.aus-nutrient-product .tab-radio{position:absolute;opacity:0;pointer-events:none}\n.aus-nutrient-product .tab-labels{display:flex;flex-wrap:wrap;gap:6px;border-bottom:2px solid #2d5a3d;margin:0 0 0 0}\n.aus-nutrient-product .tab-labels label{cursor:pointer;padding:11px 20px;background:#f0f4f1;color:#2d5a3d;font-weight:600;border-radius:10px 10px 0 0;border:1px solid #d8e1e8;border-bottom:none;font-size:14px}\n.aus-nutrient-product .tab-panel{display:none;padding:26px 4px 10px}\n#asd641-t1:checked~.tab-labels label[for=asd641-t1],#asd641-t2:checked~.tab-labels label[for=asd641-t2],#asd641-t3:checked~.tab-labels label[for=asd641-t3],#asd641-t4:checked~.tab-labels 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.scale-lbl{display:flex;justify-content:space-between;font-size:12px;color:#52606d}\n.aus-nutrient-product .swatch{display:inline-block;width:14px;height:14px;border-radius:3px;vertical-align:-2px;margin-right:6px;border:1px solid rgba(0,0,0,.15)}\n\u003c\/style\u003e\n\u003cdiv class=\"aus-nutrient-product\"\u003e\n\u003cinput type=\"radio\" name=\"asd641-tabs\" id=\"asd641-t1\" class=\"tab-radio\" checked\u003e\n\u003cinput type=\"radio\" name=\"asd641-tabs\" id=\"asd641-t2\" class=\"tab-radio\"\u003e\n\u003cinput type=\"radio\" name=\"asd641-tabs\" id=\"asd641-t3\" class=\"tab-radio\"\u003e\n\u003cinput type=\"radio\" name=\"asd641-tabs\" id=\"asd641-t4\" class=\"tab-radio\"\u003e\n\u003cdiv class=\"tab-labels\"\u003e\n\u003clabel for=\"asd641-t1\"\u003eProduct Description\u003c\/label\u003e\u003clabel for=\"asd641-t2\"\u003eTechnical Data\u003c\/label\u003e\u003clabel for=\"asd641-t3\"\u003eSafety Data\u003c\/label\u003e\u003clabel for=\"asd641-t4\"\u003eQuality \u0026amp; COA\u003c\/label\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"tab-panels\"\u003e\n\n\u003cdiv class=\"tab-panel p1\"\u003e\n\u003cdiv class=\"hero\"\u003e\u003cdiv class=\"hero-grid\"\u003e\n\u003cdiv\u003e\n\u003cspan class=\"eyebrow\"\u003eBiological Stains \u0026amp; Dyes · C.I. 16255\u003c\/span\u003e\n\u003ch1\u003ePonceau 4R (E 124)\u003c\/h1\u003e\n\u003cp class=\"subtitle\"\u003eWater-soluble red azo dye for E 124 colour reference work, spectrophotometry, analytical method development and teaching.\u003c\/p\u003e\n\u003cdiv class=\"badges\"\u003e\n\u003cspan class=\"badge b-solid\"\u003eCat. No. ASD-641\u003c\/span\u003e\u003cspan class=\"badge b-outline\"\u003eCAS 2611-82-7\u003c\/span\u003e\u003cspan class=\"badge b-outline\"\u003eDye content ≥ 80 %\u003c\/span\u003e\u003cspan class=\"badge b-green\"\u003eλmax ≈ 505 nm\u003c\/span\u003e\u003cspan class=\"badge b-gold\"\u003eFood-grade E 124\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"note-green\"\u003e\n\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Acid Red 18, Cochineal Red A, New Coccine, CI Food Red 7. Not the same dye as Ponceau S (protein stain) or Ponceau SX.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"spec-card\"\u003e\n\u003ch4\u003eAt a Glance\u003c\/h4\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cspan\u003eFormula\u003c\/span\u003e\u003cstrong\u003eC₂₀H₁₁N₂Na₃O₁₀S₃\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cspan\u003eMol. weight\u003c\/span\u003e\u003cstrong\u003e604.47 g\/mol\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cspan\u003eAppearance\u003c\/span\u003e\u003cstrong\u003eBright red powder\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cspan\u003e1 % solution\u003c\/span\u003e\u003cstrong\u003eStrawberry red\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cspan\u003eSolubility (water)\u003c\/span\u003e\u003cstrong\u003eSoluble\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cspan\u003eBatch dye content\u003c\/span\u003e\u003cstrong\u003e85.03 %\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\u003c\/div\u003e\n\n\u003cdiv class=\"main-grid\"\u003e\n\u003cdiv\u003e\n\u003ch3\u003eOverview\u003c\/h3\u003e\n\u003cp\u003ePonceau 4R is the trisodium salt of a sulfonated naphthylazo-naphthol dye. Its three sulfonate groups make it water-soluble and strongly anionic. The powder is bright red, and dilute solutions are strawberry red with an absorption maximum near 505 nm.\u003c\/p\u003e\n\u003cp\u003eIn the laboratory, Ponceau 4R is used as the reference colour for E 124 analysis, as an absorbance standard, and as a model azo dye in chromatography, sensing and decolourisation research.\u003c\/p\u003e\n\u003cp class=\"muted\"\u003eFor laboratory, research, education and quality-control use. Not intended for human or veterinary therapeutic use, diagnosis, or direct consumption. Food-grade (E 124) material. For food-industry supply, contact Ausamics for suitability and regulatory documentation.\u003c\/p\u003e\n\n\u003ch3\u003eColour at a Glance\u003c\/h3\u003e\n\u003cp\u003e\u003cspan class=\"swatch\" style=\"background:#d1202f\"\u003e\u003c\/span\u003e\u003cstrong\u003ePowder\u003c\/strong\u003e is bright red. \u003cspan class=\"swatch\" style=\"background:#f0546b\"\u003e\u003c\/span\u003e\u003cstrong\u003eSolution\u003c\/strong\u003e is strawberry red. Absorbance rises in direct proportion to concentration at 505 nm.\u003c\/p\u003e\n\u003cdiv class=\"scale\"\u003e\n\u003cspan style=\"background:#fde7ea\"\u003e\u003c\/span\u003e\u003cspan style=\"background:#fbbcc5\"\u003e\u003c\/span\u003e\u003cspan style=\"background:#f78a9a\"\u003e\u003c\/span\u003e\u003cspan style=\"background:#f0546b\"\u003e\u003c\/span\u003e\u003cspan style=\"background:#e0303f\"\u003e\u003c\/span\u003e\u003cspan style=\"background:#b3141f\"\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"scale-lbl\"\u003e\n\u003cspan\u003edilute solution\u003c\/span\u003e\u003cspan\u003epowder\u003c\/span\u003e\n\u003c\/div\u003e\n\n\u003ch3\u003eKey Applications\u003c\/h3\u003e\n\u003cdiv class=\"cards\"\u003e\n\u003cdiv class=\"card c1\"\u003e\n\u003cstrong\u003eFood Colour QC\u003c\/strong\u003eReference material for E 124 identification, HPLC and spectrophotometric assay of red food colours.\u003c\/div\u003e\n\u003cdiv class=\"card c2\"\u003e\n\u003cstrong\u003eSpectrophotometry\u003c\/strong\u003eStable red dye (λmax ≈ 505 nm) for absorbance calibration and Beer–Lambert teaching.\u003c\/div\u003e\n\u003cdiv class=\"card c3\"\u003e\n\u003cstrong\u003eAnalytical Method Development\u003c\/strong\u003eModel azo dye for chromatography, electrochemical sensing and dye-removal studies.\u003c\/div\u003e\n\u003cdiv class=\"card c1\"\u003e\n\u003cstrong\u003eEnvironmental Research\u003c\/strong\u003eTest compound in adsorption, photocatalysis and wastewater decolourisation experiments.\u003c\/div\u003e\n\u003cdiv class=\"card c2\"\u003e\n\u003cstrong\u003eEducation\u003c\/strong\u003eIntense, water-soluble red for chromatography, colour-mixing and diffusion practicals.\u003c\/div\u003e\n\u003cdiv class=\"card c3\"\u003e\n\u003cstrong\u003eFormulation Development\u003c\/strong\u003eRed colourant for prototype buffers, gels and visual tracer solutions in research.\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch3\u003eAdvantages \u0026amp; Considerations\u003c\/h3\u003e\n\u003cdiv class=\"two\"\u003e\n\u003cdiv class=\"adv\"\u003e\n\u003cstrong\u003eAdvantages\u003c\/strong\u003e\u003cul\u003e\n\u003cli\u003ePure dye content ≥ 80 % (current batch 85.03 %)\u003c\/li\u003e\n\u003cli\u003eMeets the E 124 purity limits on the batch certificate\u003c\/li\u003e\n\u003cli\u003eVery low heavy metals: lead and arsenic below 1 ppm\u003c\/li\u003e\n\u003cli\u003eReadily water-soluble; no heating needed\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"con\"\u003e\n\u003cstrong\u003eConsiderations\u003c\/strong\u003e\u003cul\u003e\n\u003cli\u003eNot a protein stain: use Ponceau S for western blot membranes\u003c\/li\u003e\n\u003cli\u003eNot permitted as a food colour in the United States\u003c\/li\u003e\n\u003cli\u003eFades under strong oxidants, reducing agents and strong light\u003c\/li\u003e\n\u003cli\u003eAvoid creating dust when weighing\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003ch3\u003eSpecification Summary\u003c\/h3\u003e\n\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePure dye content (E 1 %, 1 cm at 505 nm)\u003c\/td\u003e\n\u003ctd\u003eMin. 80.0 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance\u003c\/td\u003e\n\u003ctd\u003eBright red powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHue of 1 % solution\u003c\/td\u003e\n\u003ctd\u003eStrawberry red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLoss on drying at 135 °C (as sodium salts)\u003c\/td\u003e\n\u003ctd\u003eMax. 20.0 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMoisture at 110 °C\u003c\/td\u003e\n\u003ctd\u003eMax. 2.0 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater-insoluble matter\u003c\/td\u003e\n\u003ctd\u003eMax. 0.20 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubsidiary colouring matters\u003c\/td\u003e\n\u003ctd\u003eMax. 1.0 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOrganic compounds other than colouring matters\u003c\/td\u003e\n\u003ctd\u003eMax. 0.5 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnsulfonated primary aromatic amines (as aniline)\u003c\/td\u003e\n\u003ctd\u003eMax. 0.01 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEther-extractable matter\u003c\/td\u003e\n\u003ctd\u003eMax. 0.2 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eArsenic (As)\u003c\/td\u003e\n\u003ctd\u003eMax. 1 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLead (Pb)\u003c\/td\u003e\n\u003ctd\u003eMax. 1 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMercury (Hg)\u003c\/td\u003e\n\u003ctd\u003eMax. 0.05 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCadmium (Cd)\u003c\/td\u003e\n\u003ctd\u003eMax. 0.1 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eChromium (Cr)\u003c\/td\u003e\n\u003ctd\u003eMax. 2 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCopper (Cu)\u003c\/td\u003e\n\u003ctd\u003eMax. 30 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eZinc (Zn)\u003c\/td\u003e\n\u003ctd\u003eMax. 50 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeavy metals (as Pb)\u003c\/td\u003e\n\u003ctd\u003eMax. 5 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\n\u003ch3\u003eFrequently Asked Questions\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eIs Ponceau 4R the same as Ponceau S?\u003c\/strong\u003e\u003cbr\u003eNo. Ponceau S (C.I. 27195) is the red dye used to stain proteins on western blot membranes. Ponceau 4R (C.I. 16255) is a different azo dye, mainly used as the food colour E 124 and as an analytical reference. Do not substitute one for the other.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIs Ponceau 4R the same as Ponceau SX or FD\u0026amp;C Red No. 4?\u003c\/strong\u003e\u003cbr\u003eNo. Ponceau SX is C.I. 14700 and is sold in the US as FD\u0026amp;C Red No. 4. Ponceau 4R is C.I. 16255. The two dyes have different structures and regulatory status.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIs Ponceau 4R permitted as a food colour?\u003c\/strong\u003e\u003cbr\u003eIt is permitted in the EU as E 124 and is listed in the Australia New Zealand Food Standards Code as colour 124. It is not permitted as a food colour in the United States.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHow do I make a stock solution?\u003c\/strong\u003e\u003cbr\u003eDissolve 1.0 g in about 80 mL of purified water, stir until clear and make up to 100 mL to give a 1 % w\/v solution. Store away from light.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhat wavelength should I use for absorbance measurements?\u003c\/strong\u003e\u003cbr\u003ePonceau 4R absorbs most strongly at about 505 nm in water. Use this wavelength for calibration curves and dye-content checks.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003cdiv\u003e\n\u003cdiv class=\"side-box side-white\"\u003e\n\u003ch4\u003eTechnical Specifications\u003c\/h4\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalogue No.\u003c\/td\u003e\n\u003ctd\u003eASD-641\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eC.I. number\u003c\/td\u003e\n\u003ctd\u003e16255\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCAS \/ EC\u003c\/td\u003e\n\u003ctd\u003e2611-82-7 \/ 220-036-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE number\u003c\/td\u003e\n\u003ctd\u003eE 124\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eFormula\u003c\/td\u003e\n\u003ctd\u003eC₂₀H₁₁N₂Na₃O₁₀S₃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMol. weight\u003c\/td\u003e\n\u003ctd\u003e604.47 g\/mol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDye content\u003c\/td\u003e\n\u003ctd\u003eMin. 80 % (E 1 %, 505 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eλmax\u003c\/td\u003e\n\u003ctd\u003e≈ 505 nm (water)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage\u003c\/td\u003e\n\u003ctd\u003e15–25 °C, dry, dark, tightly closed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShelf life\u003c\/td\u003e\n\u003ctd\u003e36 months from manufacture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDocuments\u003c\/td\u003e\n\u003ctd\u003eTDS, SDS; batch COA on request\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"side-box side-green\"\u003e\n\u003ch4\u003eApplications\u003c\/h4\u003e\n\u003cul style=\"margin:0;padding-left:18px;font-size:14px\"\u003e\n\u003cli\u003eE 124 reference and QC\u003c\/li\u003e\n\u003cli\u003eSpectrophotometry standard\u003c\/li\u003e\n\u003cli\u003eHPLC method development\u003c\/li\u003e\n\u003cli\u003eDye-removal research\u003c\/li\u003e\n\u003cli\u003eTeaching laboratories\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"side-box side-gold\"\u003e\n\u003ch4\u003eStock Solution (1 % w\/v)\u003c\/h4\u003e\n\u003col class=\"steps\"\u003e\n\u003cli\u003eWeigh 1.0 g of Ponceau 4R.\u003c\/li\u003e\n\u003cli\u003eAdd to about 80 mL of purified water.\u003c\/li\u003e\n\u003cli\u003eStir until fully dissolved; no heating is needed.\u003c\/li\u003e\n\u003cli\u003eMake up to 100 mL with purified water.\u003c\/li\u003e\n\u003cli\u003eStore in a closed bottle at room temperature, away from light.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"side-box side-white\"\u003e\n\u003ch4\u003eStorage \u0026amp; Stability\u003c\/h4\u003e\n\u003cp style=\"margin:0;font-size:14px\"\u003eStore at 15–25 °C in a dry place, tightly closed and protected from sunlight, heat and moisture. Shelf life is 36 months from the date of manufacture under these conditions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"footer-note\"\u003eAusamics Pty Ltd provides this information in good faith. Users are responsible for validating this product for their own methods and regulatory requirements. Ausamics Pty Ltd does not warrant suitability for any specific application unless expressly agreed in writing.\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"tab-panel p2\"\u003e\n\u003cdiv class=\"doc-head\"\u003e\n\u003ch2\u003eTechnical Data Sheet — Ponceau 4R\u003c\/h2\u003e\n\u003cspan\u003eTDS-ASD-641 · Rev 1.0 · November 2026\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"sec\"\u003e1. Identification\u003c\/div\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003eChemical name\u003c\/td\u003e\n\u003ctd\u003eTrisodium (8Z)-7-oxo-8-[(4-sulfonatonaphthalen-1-yl)hydrazinylidene]naphthalene-1,3-disulfonate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSynonyms\u003c\/td\u003e\n\u003ctd\u003ePonceau 4R; Acid Red 18; Cochineal Red A; New Coccine; CI Food Red 7; Brilliant Scarlet 3R\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eC.I. number\u003c\/td\u003e\n\u003ctd\u003e16255\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eE number\u003c\/td\u003e\n\u003ctd\u003eE 124\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCAS number\u003c\/td\u003e\n\u003ctd\u003e2611-82-7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEC number\u003c\/td\u003e\n\u003ctd\u003e220-036-2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMolecular formula\u003c\/td\u003e\n\u003ctd\u003eC₂₀H₁₁N₂Na₃O₁₀S₃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMolecular weight\u003c\/td\u003e\n\u003ctd\u003e604.47 g\/mol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDye class\u003c\/td\u003e\n\u003ctd\u003eMonoazo, anionic (acid) dye\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"sec\"\u003e2. Intended Use\u003c\/div\u003e\n\u003cp\u003eE 124 reference colour, spectrophotometric standard, model azo dye for analytical and environmental research, and teaching reagent. For laboratory, research, education and quality-control use. Not intended for human or veterinary therapeutic use, diagnosis, or direct consumption.\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003e3. Mode of Action\u003c\/div\u003e\n\u003cp\u003eThe three sulfonate groups make Ponceau 4R strongly anionic and water-soluble. Its naphthylazo-naphthol chromophore absorbs blue-green light at about 505 nm, which gives the red colour.\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003e4. Physical \u0026amp; Chemical Properties\u003c\/div\u003e\n\u003ctable\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance\u003c\/td\u003e\n\u003ctd\u003eBright red powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eColour of 1 % aqueous solution\u003c\/td\u003e\n\u003ctd\u003eStrawberry red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAbsorption maximum (λmax)\u003c\/td\u003e\n\u003ctd\u003eapprox. 505 nm in water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolubility\u003c\/td\u003e\n\u003ctd\u003eSoluble in water; sparingly soluble in ethanol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMoisture at 110 °C\u003c\/td\u003e\n\u003ctd\u003eMax. 2.0 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStability\u003c\/td\u003e\n\u003ctd\u003eGood stability in normal laboratory conditions; protect from sunlight, heat and moisture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"sec\"\u003e5. Specifications\u003c\/div\u003e\n\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePure dye content (E 1 %, 1 cm at 505 nm)\u003c\/td\u003e\n\u003ctd\u003eMin. 80.0 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance\u003c\/td\u003e\n\u003ctd\u003eBright red powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHue of 1 % solution\u003c\/td\u003e\n\u003ctd\u003eStrawberry red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLoss on drying at 135 °C (as sodium salts)\u003c\/td\u003e\n\u003ctd\u003eMax. 20.0 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMoisture at 110 °C\u003c\/td\u003e\n\u003ctd\u003eMax. 2.0 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater-insoluble matter\u003c\/td\u003e\n\u003ctd\u003eMax. 0.20 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubsidiary colouring matters\u003c\/td\u003e\n\u003ctd\u003eMax. 1.0 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOrganic compounds other than colouring matters\u003c\/td\u003e\n\u003ctd\u003eMax. 0.5 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnsulfonated primary aromatic amines (as aniline)\u003c\/td\u003e\n\u003ctd\u003eMax. 0.01 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEther-extractable matter\u003c\/td\u003e\n\u003ctd\u003eMax. 0.2 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eArsenic (As)\u003c\/td\u003e\n\u003ctd\u003eMax. 1 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLead (Pb)\u003c\/td\u003e\n\u003ctd\u003eMax. 1 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMercury (Hg)\u003c\/td\u003e\n\u003ctd\u003eMax. 0.05 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCadmium (Cd)\u003c\/td\u003e\n\u003ctd\u003eMax. 0.1 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eChromium (Cr)\u003c\/td\u003e\n\u003ctd\u003eMax. 2 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCopper (Cu)\u003c\/td\u003e\n\u003ctd\u003eMax. 30 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eZinc (Zn)\u003c\/td\u003e\n\u003ctd\u003eMax. 50 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeavy metals (as Pb)\u003c\/td\u003e\n\u003ctd\u003eMax. 5 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv class=\"sec\"\u003e6. Solution Preparation\u003c\/div\u003e\n\u003cp\u003eStock solution (1 % w\/v): dissolve 1.0 g in about 80 mL purified water and make up to 100 mL. Dilute for use according to your method.\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003e7. Storage \u0026amp; Shelf Life\u003c\/div\u003e\n\u003cp\u003e15–25 °C, dry, tightly closed, protected from sunlight, heat and moisture. Shelf life 36 months from manufacture.\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003e8. References\u003c\/div\u003e\n\u003col class=\"muted\"\u003e\n\u003cli\u003eCommission Regulation (EU) No 231\/2012, Annex: E 124 Ponceau 4R, Cochineal Red A. Specifications for food additives.\u003c\/li\u003e\n\u003cli\u003eFAO\/WHO JECFA (2011). Ponceau 4R: specifications and toxicological evaluation (74th meeting). ADI 0–4 mg\/kg body weight.\u003c\/li\u003e\n\u003cli\u003eEFSA ANS Panel (2009). Scientific Opinion on the re-evaluation of Ponceau 4R (E 124) as a food additive. EFSA Journal 7(11):1328.\u003c\/li\u003e\n\u003cli\u003eRegulation (EC) No 1333\/2008 on food additives, Annex V: additional labelling for foods containing E 102, E 104, E 110, E 122, E 124 and E 129.\u003c\/li\u003e\n\u003cli\u003eHorobin R.W., Kiernan J.A. (eds) (2002). Conn's Biological Stains, 10th ed. BIOS Scientific Publishers, Oxford.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"tab-panel p3\"\u003e\n\u003cdiv class=\"doc-head\"\u003e\n\u003ch2\u003eSafety Data Summary — Ponceau 4R\u003c\/h2\u003e\n\u003cspan\u003eSDS-ASD-641 · Rev 1.0 · November 2026 · Full 16-section SDS available on request\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"sec\"\u003eHazard Classification\u003c\/div\u003e\n\u003cp\u003eNot classified as hazardous according to the criteria of the GHS (Rev. 7) as adopted under the Australian Work Health and Safety Regulations. No hazard pictogram or signal word is required.\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003eHandling \u0026amp; Protection\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003eAvoid creating and breathing dust. Use with adequate ventilation.\u003c\/li\u003e\n\u003cli\u003eWear nitrile gloves, laboratory coat and safety glasses.\u003c\/li\u003e\n\u003cli\u003eThe dye stains skin and surfaces; clean spills promptly with water.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv class=\"sec\"\u003eFirst Aid\u003c\/div\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eInhalation:\u003c\/strong\u003e Move to fresh air.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSkin:\u003c\/strong\u003e Wash with soap and water.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eEyes:\u003c\/strong\u003e Rinse with water for several minutes. Seek medical advice if irritation persists.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIngestion:\u003c\/strong\u003e Rinse mouth. Seek medical advice if you feel unwell.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"muted\"\u003ePoisons Information Centre (Australia): 13 11 26 · Emergency: 000\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003eStorage \u0026amp; Disposal\u003c\/div\u003e\n\u003cp\u003eStore at 15–25 °C, dry, tightly closed and protected from light. Dispose of according to local regulations.\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003eTransport\u003c\/div\u003e\n\u003cp\u003eNot regulated as dangerous goods for road (ADG), sea (IMDG) or air (IATA) transport.\u003c\/p\u003e\n\u003c\/div\u003e\n\n\u003cdiv class=\"tab-panel p4\"\u003e\n\u003cdiv class=\"doc-head\"\u003e\n\u003ch2\u003eQuality \u0026amp; Certificate of Analysis\u003c\/h2\u003e\n\u003cspan\u003eASD-641 · Batch-specific COA available on request\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cp\u003eEach batch is supplied with traceable manufacturer test data. A batch-specific Certificate of Analysis is available on request. Please quote the batch number printed on the label.\u003c\/p\u003e\n\u003cdiv class=\"sec\"\u003eTypical Batch Results\u003c\/div\u003e\n\u003ctable\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth\u003eTest\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eResult\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003ePure dye content (E 1 %, 1 cm at 505 nm)\u003c\/td\u003e\n\u003ctd\u003eMin. 80.0 %\u003c\/td\u003e\n\u003ctd\u003e85.03 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance\u003c\/td\u003e\n\u003ctd\u003eBright red powder\u003c\/td\u003e\n\u003ctd\u003eBright red powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHue of 1 % solution\u003c\/td\u003e\n\u003ctd\u003eStrawberry red\u003c\/td\u003e\n\u003ctd\u003eStrawberry red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLoss on drying at 135 °C (as sodium salts)\u003c\/td\u003e\n\u003ctd\u003eMax. 20.0 %\u003c\/td\u003e\n\u003ctd\u003e12.99 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMoisture at 110 °C\u003c\/td\u003e\n\u003ctd\u003eMax. 2.0 %\u003c\/td\u003e\n\u003ctd\u003e0.88 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWater-insoluble matter\u003c\/td\u003e\n\u003ctd\u003eMax. 0.20 %\u003c\/td\u003e\n\u003ctd\u003e0.08 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubsidiary colouring matters\u003c\/td\u003e\n\u003ctd\u003eMax. 1.0 %\u003c\/td\u003e\n\u003ctd\u003e0.45 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOrganic compounds other than colouring matters\u003c\/td\u003e\n\u003ctd\u003eMax. 0.5 %\u003c\/td\u003e\n\u003ctd\u003e0.35 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eUnsulfonated primary aromatic amines (as aniline)\u003c\/td\u003e\n\u003ctd\u003eMax. 0.01 %\u003c\/td\u003e\n\u003ctd\u003e\u0026lt; 0.01 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEther-extractable matter\u003c\/td\u003e\n\u003ctd\u003eMax. 0.2 %\u003c\/td\u003e\n\u003ctd\u003e0.08 %\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eArsenic (As)\u003c\/td\u003e\n\u003ctd\u003eMax. 1 ppm\u003c\/td\u003e\n\u003ctd\u003e\u0026lt; 1 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLead (Pb)\u003c\/td\u003e\n\u003ctd\u003eMax. 1 ppm\u003c\/td\u003e\n\u003ctd\u003e\u0026lt; 1 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMercury (Hg)\u003c\/td\u003e\n\u003ctd\u003eMax. 0.05 ppm\u003c\/td\u003e\n\u003ctd\u003e0.005 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCadmium (Cd)\u003c\/td\u003e\n\u003ctd\u003eMax. 0.1 ppm\u003c\/td\u003e\n\u003ctd\u003e0.04 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eChromium (Cr)\u003c\/td\u003e\n\u003ctd\u003eMax. 2 ppm\u003c\/td\u003e\n\u003ctd\u003e\u0026lt; 1 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCopper (Cu)\u003c\/td\u003e\n\u003ctd\u003eMax. 30 ppm\u003c\/td\u003e\n\u003ctd\u003e20 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eZinc (Zn)\u003c\/td\u003e\n\u003ctd\u003eMax. 50 ppm\u003c\/td\u003e\n\u003ctd\u003e30 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeavy metals (as Pb)\u003c\/td\u003e\n\u003ctd\u003eMax. 5 ppm\u003c\/td\u003e\n\u003ctd\u003e\u0026lt; 5 ppm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"muted\"\u003eResults as reported on the manufacturer's certificate of analysis dated 19\/10\/2026.\u003c\/p\u003e\n\u003cdiv class=\"footer-note\"\u003eThe results reported in a Certificate of Analysis apply only to the batch identified on it. A certificate may not be reproduced except in full without prior written approval from Ausamics Pty Ltd. The user is responsible for determining the suitability of this product for their intended application and for ensuring its proper use, handling, and storage. Ausamics Pty Ltd does not warrant the suitability or performance of this product for any specific application unless expressly agreed in writing. To the extent permitted by applicable law, Ausamics Pty Ltd accepts no liability for loss or damage arising from improper use or handling of this product.\u003c\/div\u003e\n\u003c\/div\u003e\n\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"AuSaMicS","offers":[{"title":"25 g","offer_id":51038439670015,"sku":"ASD-641-25G","price":25.0,"currency_code":"AUD","in_stock":true},{"title":"100 g","offer_id":51038439702783,"sku":"ASD-641-100G","price":39.0,"currency_code":"AUD","in_stock":true},{"title":"250 g","offer_id":51038439735551,"sku":"ASD-641-250G","price":67.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":51038439768319,"sku":"ASD-641-500G","price":97.0,"currency_code":"AUD","in_stock":true},{"title":"1 kg","offer_id":51038439801087,"sku":"ASD-641-1KG","price":169.0,"currency_code":"AUD","in_stock":true},{"title":"5 kg","offer_id":51038439964927,"sku":"ASD-641-5KG","price":699.0,"currency_code":"AUD","in_stock":true}],"url":"https:\/\/ausamics.com.au\/products\/ponceau-4r-e124-food-grade-red-colour-powder","provider":"AuSaMicS","version":"1.0","type":"link"}