{"product_id":"pyruvate-broth","title":"Pyruvate Broth","description":"\u003cdiv style=\"font-family: 'Segoe UI', system-ui, -apple-system, sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\" class=\"aus-nutrient-product\"\u003e\n\u003cdiv style=\"background: linear-gradient(135deg, #f0f4f8 0%, #d9e2ec 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #d8e1e8; border-left: 8px solid #2d5a3d;\" class=\"aus-hero-section\"\u003e\n\u003cdiv class=\"aus-hero-grid\" style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #2d5a3d; font-weight: bold; margin-bottom: 12px; background: #fff; padding: 6px 12px; border-radius: 20px; border: 1px solid #d8e1e8;\"\u003eAuSaMicS Life Science • Differential Media\u003c\/div\u003e\n\u003ch1 style=\"color: #15324b; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003ePyruvate Broth\u003c\/h1\u003e\n\u003ch2 style=\"color: #243b53; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: 0.9;\"\u003eConfirmatory Test for Enterococcus spp.\u003c\/h2\u003e\n\u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #334e68; max-width: 600px; font-weight: 500;\"\u003eSpecific liquid medium designed to detect \u003cstrong\u003epyruvate utilization\u003c\/strong\u003e. Essential for differentiating Enterococcus species from group D streptococci through clear-cut acid production and color change.\u003c\/p\u003e\n\u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n\u003cspan style=\"background: #2d5a3d; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAS-1335\u003c\/span\u003e \u003cspan style=\"background: #fff; border: 2px solid #28a745; color: #28a745; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🧪 Differential\u003c\/span\u003e \u003cspan style=\"background: #28a745; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e✓ FDA-BAM Compliant\u003c\/span\u003e \u003cspan style=\"background: #c9a84c; color: #1a1a1a; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e🥼 Enterococci Control\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #4a5568;\"\u003e\n\u003cspan\u003e\u003cstrong\u003eTarget:\u003c\/strong\u003e Enterococcus spp.\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eIndicator:\u003c\/strong\u003e Bromocresol Purple\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eStandard:\u003c\/strong\u003e FDA-BAM Chapter 5\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"aus-guarantee\" style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 280px; border: 1px solid #d8e1e8;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003e Differentiation Specialist\u003c\/h4\u003e\n\u003cdiv style=\"font-size: 14px; color: #4a5568; text-align: center; line-height: 1.5;\"\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eReliable Confirmation\u003c\/strong\u003e - Single-tube test\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eClinical Grade\u003c\/strong\u003e - Identification algorithms\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eClear Interpretation\u003c\/strong\u003e - Purple to Yellow\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eFast Supply\u003c\/strong\u003e - Ships within 24h\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e\n\u003cstrong\u003eValidated\u003c\/strong\u003e - FDA-BAM \u0026amp; National standards\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-top: 20px; padding: 15px; background: #f0f4f1; border-radius: 12px; text-align: center; border: 1px solid #c8d8cc;\"\u003e\n\u003cdiv style=\"color: #2d5a3d; font-weight: 600; font-size: 14px;\"\u003eHigh Specificity\u003c\/div\u003e\n\u003cdiv style=\"color: #4a5568; font-size: 13px; margin-top: 5px;\"\u003eSodium Pyruvate Base\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"aus-main-grid\" style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003e🧪 Complete Formulation \u0026amp; Nutrient Profile\u003c\/h3\u003e\n\u003cdiv style=\"background: #f8fafc; padding: 20px; border-radius: 16px; border-left: 5px solid #1d6fb8; margin-bottom: 20px;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px;\"\u003e📋 Ingredient Composition (per Liter)\u003c\/h4\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #d8e1e8; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n\u003cthead style=\"background: #f6f8fb;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eIngredient\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eConcentration\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003ePrimary Function\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eProteose Peptone \/ Tryptone\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #2d5a3d; font-weight: 600;\"\u003e10.0 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eNitrogen \u0026amp; Amino Acid source\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eYeast Extract\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #c9a84c; font-weight: 600;\"\u003e5.0 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eB-complex vitamins \u0026amp; growth factors\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eSodium Pyruvate\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; color: #1d6fb8; font-weight: 600;\"\u003e10.0 - 15.0 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eSole fermentable carbon source\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eSodium Chloride\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003e5.0 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003eOsmotic equilibrium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; font-weight: 600;\"\u003eBromocresol Purple\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003e0.02 g\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003epH Indicator (Purple to Yellow)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d8e1e8;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 15px 0; font-size: 16px;\"\u003e⚗️ Specialized Detection System\u003c\/h4\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(200px, 1fr)); gap: 15px;\"\u003e\n\u003cdiv style=\"background: #f0f4f1; padding: 15px; border-radius: 12px; border-left: 4px solid #2d5a3d;\"\u003e\n\u003cstrong style=\"color: #2d5a3d;\"\u003eCarbon Specificity\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003ePyruvate is the restricted energy source for acid production\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff9e6; padding: 15px; border-radius: 12px; border-left: 4px solid #c9a84c;\"\u003e\n\u003cstrong style=\"color: #c9a84c;\"\u003eIndicator Precision\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eBromocresol purple offers clear visual distinction at pH shift\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #e8f2ff; padding: 15px; border-radius: 12px; border-left: 4px solid #1565c0;\"\u003e\n\u003cstrong style=\"color: #1565c0;\"\u003eNutrient Balance\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eYeast extract ensures growth of fastidious streptococci\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #f0f4f1; padding: 15px; border-radius: 10px; margin-top: 15px; border: 1px solid #c8d8cc;\"\u003e\n\u003cstrong style=\"color: #2d5a3d;\"\u003eReliable Differentiation:\u003c\/strong\u003e Effectively separates \u003cem\u003eEnterococcus\u003c\/em\u003e (Positive) from \u003cem\u003eS. bovis\/S. equinus\u003c\/em\u003e (Negative), critical for clinical diagnostics.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003eEnterococci Confirmatory Media Portfolio\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #d8e1e8; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05); min-width: 1000px;\"\u003e\n\u003cthead style=\"background: #f6f8fb;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eMedium\u003c\/th\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eTarget Reaction\u003c\/th\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eSpecificity\u003c\/th\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eForm\u003c\/th\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #d8e1e8; text-align: left; font-weight: bold;\"\u003eBest Application\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #f0f4f1; border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; color: #2d5a3d;\"\u003e\u003ca style=\"color: #2d5a3d; text-decoration: none;\" href=\"#\"\u003ePyruvate Broth\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #28a745; font-weight: 600;\"\u003ePyruvate Utilization\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #28a745; font-weight: 600;\"\u003eHigh (Enterococcus)\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eBroth\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eSpeciation of Group D Strep\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003e\u003ca style=\"color: #1976d2; text-decoration: none; font-weight: 600;\" href=\"#\"\u003eBile Esculin Azide\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eEsculin Hydrolysis\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #17a2b8;\"\u003eBroad (Group D)\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eAgar\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003ePrimary isolation\/screening\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003e\u003ca style=\"color: #e91e63; text-decoration: none; font-weight: 600;\" href=\"#\"\u003e6.5% NaCl Broth\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eSalt Tolerance\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #28a745; font-weight: 600;\"\u003eHigh (Enterococcus)\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eBroth\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eDifferential confirmatory test\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #15324b; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #1d6fb8;\"\u003eStrategic Selection for Enterococci Confirmation\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(300px, 1fr)); gap: 20px; margin-bottom: 25px;\"\u003e\n\u003cdiv style=\"background: #e8f5e8; padding: 20px; border-radius: 16px; border: 1px solid #c8e6c9;\"\u003e\n\u003ch4 style=\"color: #2e7d32; margin: 0 0 15px 0; font-size: 16px;\"\u003e✅ Pyruvate Broth Advantages\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003eHighly Specific:\u003c\/strong\u003e Reliable speciation\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003eClear-cut Results:\u003c\/strong\u003e Sharp color change\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003eStandardized:\u003c\/strong\u003e Follows FDA-BAM protocols\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #2e7d32;\"\u003e✓ \u003cstrong\u003eVersatile:\u003c\/strong\u003e Clinical and food microbiology\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff3e0; padding: 20px; border-radius: 16px; border: 1px solid #ffcc02;\"\u003e\n\u003ch4 style=\"color: #e65100; margin: 0 0 15px 0; font-size: 16px;\"\u003e⚠️ Method Considerations\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #e65100;\"\u003e▲ \u003cstrong\u003eRare Isolates:\u003c\/strong\u003e Leuconostoc may show weak positive\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #e65100;\"\u003e▲ \u003cstrong\u003eIncubation:\u003c\/strong\u003e Requires up to 48 hours for confirmation\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0; font-size: 14px; color: #e65100;\"\u003e▲ \u003cstrong\u003eLight Sensitivity:\u003c\/strong\u003e Indicator must be protected\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #d8e1e8; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003e📋 Technical Specifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; width: 45%; background: #f6f8fb;\"\u003eCatalogue Number\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eAS-1335\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eFinal pH\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e6.9 ± 0.1 (at 25°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eControl Indicator\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eBromocresol Purple\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eSterilization\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #d9534f; font-weight: 600;\"\u003e121°C, 15 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #d8e1e8;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #15324b; background: #f6f8fb;\"\u003eShelf Life (Prep)\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #28a745; font-weight: 600;\"\u003e3 months (2-8°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #f0f4f1; padding: 20px; border-radius: 16px; border: 1px solid #c8d8cc; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #2d5a3d; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e🔍 Interpretation Guide\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #2d5a3d;\"\u003e🟡 \u003cstrong\u003ePositive:\u003c\/strong\u003e Yellow color (Acid produced) - \u003cem\u003eEnterococcus spp.\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #5d4037;\"\u003e🟣 \u003cstrong\u003eNegative:\u003c\/strong\u003e Remains Purple - \u003cem\u003eS. bovis, S. equinus\u003c\/em\u003e\n\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 13px; color: #6c757d; font-style: italic;\"\u003e*Read at 24h and 48h for final confirmation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff9e6; padding: 20px; border-radius: 16px; border: 1px solid #c9a84c; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #c9a84c; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e⚗️ Preparation Protocol\u003c\/h4\u003e\n\u003col style=\"list-style: none; padding: 0; margin: 0; counter-reset: steps;\"\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #5d4037; counter-increment: steps; position: relative; padding-left: 30px;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; top: 8px; background: #c9a84c; color: #fff; width: 20px; height: 20px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 12px; font-weight: bold;\"\u003e1\u003c\/span\u003e Suspend \u003cstrong\u003e30.0 - 35.0g\u003c\/strong\u003e in 1L purified water\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #5d4037; counter-increment: steps; position: relative; padding-left: 30px;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; top: 8px; background: #c9a84c; color: #fff; width: 20px; height: 20px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 12px; font-weight: bold;\"\u003e2\u003c\/span\u003e Dispense 4-5 mL into small tubes\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #5d4037; counter-increment: steps; position: relative; padding-left: 30px;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; top: 8px; background: #c9a84c; color: #fff; width: 20px; height: 20px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 12px; font-weight: bold;\"\u003e3\u003c\/span\u003e Autoclave at \u003cstrong\u003e121°C for 15 min\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #5d4037; counter-increment: steps; position: relative; padding-left: 30px;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; top: 8px; background: #c9a84c; color: #fff; width: 20px; height: 20px; border-radius: 50%; display: flex; align-items: center; justify-content: center; font-size: 12px; font-weight: bold;\"\u003e4\u003c\/span\u003e Incubate at \u003cstrong\u003e35-37°C\u003c\/strong\u003e for 18-48h\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Benario","offers":[{"title":"Default Title","offer_id":48030974935295,"sku":"AS-1335-DH-LS-100G","price":45.0,"currency_code":"AUD","in_stock":true}],"url":"https:\/\/ausamics.com.au\/products\/pyruvate-broth","provider":"AuSaMicS","version":"1.0","type":"link"}