{"product_id":"sim-medium-sulfide-indole-motility-medium","title":"SIM Medium | AS-1347 | Sulfide Indole Motility Agar | AuSaMicS","description":"\u003cbody\u003e\n\n\n    \u003cmeta charset=\"UTF-8\"\u003e\n    \u003cmeta name=\"viewport\" content=\"width=device-width, initial-scale=1.0\"\u003e\n    \u003ctitle\u003eSIM Medium | AS-1347 | Sulfide Indole Motility | AuSaMicS\u003c\/title\u003e\n    \u003cstyle\u003e\n        :root { --ink:#15324b; --muted:#5d6c7a; --line:#d8e1e8; --soft:#f6f8fb; --brand:#123d6b; --accent:#1d6fb8; --ok:#e8f2ff; --sulfide:#212121; --indole:#d32f2f; --motile:#4caf50; }\n        * { box-sizing: border-box; }\n        body { margin: 0; font-family: 'Segoe UI', Arial, sans-serif; color: #1d2935; background: #ffffff; line-height: 1.6; }\n        .aus-wrap { max-width: 1040px; margin: 0 auto; padding: 32px 20px 56px; }\n        \n        \/* Hero Section *\/\n        .aus-hero { background: linear-gradient(135deg, #eceff1 0%, #cfd8dc 100%); border: 1px solid var(--line); border-radius: 18px; padding: 35px; margin-bottom: 24px; border-left: 8px solid var(--brand); }\n        .aus-eyebrow { display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: var(--brand); font-weight: 700; margin-bottom: 10px; background: #fff; padding: 4px 12px; border-radius: 20px; border: 1px solid var(--line); }\n        h1 { font-size: 32px; color: var(--ink); margin: 0 0 10px; }\n        .aus-badges { display: flex; flex-wrap: wrap; gap: 10px; margin-top: 15px; }\n        .aus-badge { background: #fff; border: 1px solid var(--line); padding: 6px 14px; border-radius: 999px; font-size: 13px; font-weight: 600; color: var(--ink); }\n\n        \/* Grid Layout *\/\n        .aus-grid { display: grid; grid-template-columns: 1.2fr .8fr; gap: 20px; margin-top: 20px; }\n        @media (max-width: 860px) { .aus-grid { grid-template-columns: 1fr; } }\n        .aus-card { background: #fff; border: 1px solid var(--line); border-radius: 16px; padding: 25px; height: 100%; }\n        \n        \/* Tables *\/\n        .aus-table-wrap { margin: 20px 0; border: 1px solid var(--line); border-radius: 12px; overflow: hidden; }\n        table { width: 100%; border-collapse: collapse; font-size: 14px; }\n        th, td { padding: 12px; border-bottom: 1px solid var(--line); text-align: left; vertical-align: top; }\n        th { background: var(--soft); color: var(--ink); font-weight: 700; }\n        .dark-th th { background: var(--brand); color: #fff; }\n        \n        \/* Typography *\/\n        h2 { font-size: 22px; color: var(--brand); margin: 30px 0 15px; border-bottom: 2px solid var(--ok); padding-bottom: 5px; }\n        h3 { font-size: 18px; color: var(--ink); margin-top: 0; }\n        .aus-list { padding-left: 20px; }\n        .aus-list li { margin-bottom: 8px; }\n        \n        \/* Reaction Indicators *\/\n        .reaction-grid { display: grid; grid-template-columns: repeat(3, 1fr); gap: 10px; margin: 15px 0; }\n        .reaction-chip { padding: 10px; border-radius: 8px; text-align: center; font-size: 12px; font-weight: bold; color: white; }\n        .sulfide { background: var(--sulfide); }\n        .indole { background: var(--indole); }\n        .motile { background: var(--motile); }\n\n        .footer { margin-top: 40px; padding-top: 20px; border-top: 1px solid var(--line); font-size: 13px; color: var(--muted); text-align: center; }\n    \u003c\/style\u003e\n\n\n\n\u003cdiv class=\"aus-wrap\"\u003e\n    \u003cheader class=\"aus-hero\"\u003e\n        \u003cdiv class=\"aus-eyebrow\"\u003eAuSaMicS Life Science • Multiparametric Media\u003c\/div\u003e\n        \u003ch1\u003eSIM Medium\u003c\/h1\u003e\n        \u003cp\u003e\u003cstrong\u003eA comprehensive 3-in-1 differential medium for the simultaneous determination of Hydrogen Sulfide (H₂S) production, Indole formation, and bacterial Motility.\u003c\/strong\u003e\u003c\/p\u003e\n        \u003cdiv class=\"aus-badges\"\u003e\n            \u003cspan class=\"aus-badge\"\u003eCat No: AS-1347\u003c\/span\u003e\n            \u003cspan class=\"aus-badge\"\u003eISO 6579 Compliant\u003c\/span\u003e\n            \u003cspan class=\"aus-badge\"\u003eFDA-BAM Validated\u003c\/span\u003e\n            \u003cspan class=\"aus-badge\"\u003e3-in-1 Diagnostic\u003c\/span\u003e\n        \u003c\/div\u003e\n    \u003c\/header\u003e\n\n    \u003cdiv class=\"aus-grid\"\u003e\n        \u003cdiv class=\"aus-card\"\u003e\n            \u003ch3\u003eOverview \u0026amp; Diagnostic Utility\u003c\/h3\u003e\n            \u003cp\u003eSIM Medium (Sulfide Indole Motility) is a semi-solid diagnostic tool vital for identifying members of the \u003cem\u003eEnterobacteriaceae\u003c\/em\u003e family. By combining three tests into a single tube, it streamlines workflows for \u003cem\u003eSalmonella\u003c\/em\u003e and \u003cem\u003eShigella\u003c\/em\u003e identification, saving time and resources in clinical and food safety laboratories.\u003c\/p\u003e\n            \n            \u003cdiv class=\"reaction-grid\"\u003e\n                \u003cdiv class=\"reaction-chip sulfide\"\u003eH₂S PRODUCTION\u003c\/div\u003e\n                \u003cdiv class=\"reaction-chip indole\"\u003eINDOLE FORMATION\u003c\/div\u003e\n                \u003cdiv class=\"reaction-chip motile\"\u003eMOTILITY\u003c\/div\u003e\n            \u003c\/div\u003e\n\n            \u003ch3\u003eKey Features\u003c\/h3\u003e\n            \u003cul class=\"aus-list\"\u003e\n                \u003cli\u003e\n\u003cstrong\u003eMotility Detection:\u003c\/strong\u003e Reduced agar concentration (0.35%) allows motile organisms to move through the medium, creating visible turbidity.\u003c\/li\u003e\n                \u003cli\u003e\n\u003cstrong\u003eH₂S Sensitivity:\u003c\/strong\u003e Ferrous ammonium sulfate provides a highly sensitive indicator for black precipitate formation.\u003c\/li\u003e\n                \u003cli\u003e\n\u003cstrong\u003eTryptophan Rich:\u003c\/strong\u003e Optimized peptone content ensures reliable indole testing with Kovac’s reagent.\u003c\/li\u003e\n            \u003c\/ul\u003e\n        \u003c\/div\u003e\n\n        \u003cdiv class=\"aus-card\"\u003e\n            \u003ch3\u003eTechnical Specifications\u003c\/h3\u003e\n            \u003cdiv class=\"aus-table-wrap\"\u003e\n                \u003ctable\u003e\n                    \u003ctr\u003e\n\u003cth\u003eFinal pH\u003c\/th\u003e\n\u003ctd\u003e7.3 ± 0.2 (at 25°C)\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSemi-solid deeps\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003cth\u003eInoculation\u003c\/th\u003e\n\u003ctd\u003eStraight Stab\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003cth\u003eIncubation\u003c\/th\u003e\n\u003ctd\u003e35-37°C (18-48h)\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003cth\u003eStorage (Powder)\u003c\/th\u003e\n\u003ctd\u003e15–30°C (Dry)\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003cth\u003eStorage (Prepared)\u003c\/th\u003e\n\u003ctd\u003e2–8°C (Up to 6 mos)\u003c\/td\u003e\n\u003c\/tr\u003e\n                \u003c\/table\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003csection\u003e\n        \u003ch2\u003eIdentification Matrix: SIM Results\u003c\/h2\u003e\n        \u003cdiv class=\"aus-table-wrap\"\u003e\n            \u003ctable\u003e\n                \u003cthead\u003e\n                    \u003ctr class=\"dark-th\"\u003e\n                        \u003cth\u003eOrganism\u003c\/th\u003e\n                        \u003cth\u003eSulfide (H₂S)\u003c\/th\u003e\n                        \u003cth\u003eIndole\u003c\/th\u003e\n                        \u003cth\u003eMotility\u003c\/th\u003e\n                    \u003c\/tr\u003e\n                \u003c\/thead\u003e\n                \u003ctbody\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003e\u003cem\u003eEscherichia coli\u003c\/em\u003e\u003c\/td\u003e\n                        \u003ctd\u003eNegative (-)\u003c\/td\u003e\n                        \u003ctd style=\"color:var(--indole); font-weight:bold;\"\u003ePositive (+)\u003c\/td\u003e\n                        \u003ctd style=\"color:var(--motile); font-weight:bold;\"\u003ePositive (+)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003e\u003cem\u003eSalmonella Typhi\u003c\/em\u003e\u003c\/td\u003e\n                        \u003ctd style=\"color:var(--sulfide); font-weight:bold;\"\u003ePositive (+)\u003c\/td\u003e\n                        \u003ctd\u003eNegative (-)\u003c\/td\u003e\n                        \u003ctd style=\"color:var(--motile); font-weight:bold;\"\u003ePositive (+)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003e\u003cem\u003eShigella dysenteriae\u003c\/em\u003e\u003c\/td\u003e\n                        \u003ctd\u003eNegative (-)\u003c\/td\u003e\n                        \u003ctd\u003eVaries (+\/-)\u003c\/td\u003e\n                        \u003ctd\u003eNegative (-)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003e\u003cem\u003eProteus mirabilis\u003c\/em\u003e\u003c\/td\u003e\n                        \u003ctd style=\"color:var(--sulfide); font-weight:bold;\"\u003ePositive (+)\u003c\/td\u003e\n                        \u003ctd\u003eNegative (-)\u003c\/td\u003e\n                        \u003ctd style=\"color:var(--motile); font-weight:bold;\"\u003ePositive (+)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                \u003c\/tbody\u003e\n            \u003c\/table\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \u003csection\u003e\n        \u003ch2\u003ePractical Evaluation\u003c\/h2\u003e\n        \u003cdiv class=\"aus-grid\"\u003e\n            \u003cdiv class=\"aus-card\"\u003e\n                \u003ch3\u003ePros\u003c\/h3\u003e\n                \u003cul class=\"aus-list\"\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eResource Efficient:\u003c\/strong\u003e Replaces three separate tubes with one, reducing incubator space and plastic waste.\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eClear Motility:\u003c\/strong\u003e Much easier to interpret than standard broth or full-agar slants.\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eStandardized:\u003c\/strong\u003e Compatible with API 20E systems and international standards (ISO\/USP).\u003c\/li\u003e\n                \u003c\/ul\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"aus-card\"\u003e\n                \u003ch3\u003eCons\u003c\/h3\u003e\n                \u003cul class=\"aus-list\"\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eTechnique Sensitive:\u003c\/strong\u003e Requires a perfect vertical stab; sideways movement can mimic false motility.\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eReagent Required:\u003c\/strong\u003e Indole results are not visible until Kovac's or Ehrlich's reagent is added post-incubation.\u003c\/li\u003e\n                \u003c\/ul\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \u003csection\u003e\n        \u003ch2\u003ePreparation \u0026amp; Composition\u003c\/h2\u003e\n        \u003cdiv class=\"aus-grid\"\u003e\n            \u003cdiv class=\"aus-table-wrap\"\u003e\n                \u003ctable\u003e\n                    \u003ctr class=\"dark-th\"\u003e\n\u003cth\u003eIngredient\u003c\/th\u003e\n\u003cth\u003eGrams\/Litre\u003c\/th\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd\u003ePeptone Mixture\u003c\/td\u003e\n\u003ctd\u003e30.0 g\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd\u003eBeef Extract\u003c\/td\u003e\n\u003ctd\u003e3.0 g\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd\u003eFerrous Ammonium Sulfate\u003c\/td\u003e\n\u003ctd\u003e0.2 g\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd\u003eSodium Thiosulfate\u003c\/td\u003e\n\u003ctd\u003e0.025 g\u003c\/td\u003e\n\u003c\/tr\u003e\n                    \u003ctr\u003e\n\u003ctd\u003eAgar (Semi-solid)\u003c\/td\u003e\n\u003ctd\u003e3.5 g\u003c\/td\u003e\n\u003c\/tr\u003e\n                \u003c\/table\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"aus-card\"\u003e\n                \u003ch3\u003eProtocol\u003c\/h3\u003e\n                \u003col class=\"aus-list\"\u003e\n                    \u003cli\u003eSuspend \u003cstrong\u003e36.725g\u003c\/strong\u003e of AS-1347 in 1L of purified water.\u003c\/li\u003e\n                    \u003cli\u003eHeat with agitation until completely dissolved.\u003c\/li\u003e\n                    \u003cli\u003eDispense into tubes as \u003cstrong\u003edeep butts\u003c\/strong\u003e (no slants).\u003c\/li\u003e\n                    \u003cli\u003eAutoclave at \u003cstrong\u003e121°C for 15 minutes\u003c\/strong\u003e.\u003c\/li\u003e\n                    \u003cli\u003eCool in an \u003cstrong\u003eupright position\u003c\/strong\u003e to ensure a uniform column.\u003c\/li\u003e\n                \u003c\/ol\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \u003cfooter class=\"footer\"\u003e\n        AuSaMicS Pty Ltd • 31 Longview CT, Thomastown, VIC 3074, Australia • ABN 56676640467\u003cbr\u003e\n        www.ausamics.com.au • support@ausamics.com • Reference: ISO 6579 \/ FDA-BAM\n    \u003c\/footer\u003e\n\u003c\/div\u003e\n\n\n\u003c\/body\u003e","brand":"Ausamics Life Science","offers":[{"title":"100 g","offer_id":48383931875583,"sku":"AS-1347-100G","price":45.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":48383931908351,"sku":"AS-1347-500G","price":135.0,"currency_code":"AUD","in_stock":true},{"title":"2.5 kg","offer_id":48383931941119,"sku":"AS-1347-2.5KG","price":545.0,"currency_code":"AUD","in_stock":true},{"title":"10 kg","offer_id":48383931973887,"sku":"AS-1347-10KG","price":1995.0,"currency_code":"AUD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1087\/0271\/files\/SIM_Medium_AS-1347_Sulfide_Indole_Motility_Agar.jpg?v=1775729534","url":"https:\/\/ausamics.com.au\/products\/sim-medium-sulfide-indole-motility-medium","provider":"AuSaMicS","version":"1.0","type":"link"}