{"product_id":"streptococcus-selection-broth-pike-as-1450","title":"Streptococcus Selection Broth (Pike) AS-1450","description":"\u003cstyle\u003e\n.aus-nutrient-product .aus-tab-input{position:absolute;opacity:0;pointer-events:none}\n.aus-nutrient-product .aus-tabbar{display:flex;flex-wrap:wrap;gap:8px;border-bottom:3px solid #1d6fb8;margin:0 0 30px 0}\n.aus-nutrient-product .aus-tabbar label{cursor:pointer;padding:12px 20px;border-radius:12px 12px 0 0;background:#f0f4f8;border:1px solid #d8e1e8;border-bottom:none;color:#15324b;font-weight:600;font-size:14px}\n.aus-nutrient-product .aus-panel{display:none}\n#aus-t1:checked ~ .aus-tabbar label[for=\"aus-t1\"],#aus-t2:checked ~ .aus-tabbar label[for=\"aus-t2\"],#aus-t3:checked ~ .aus-tabbar label[for=\"aus-t3\"],#aus-t4:checked ~ .aus-tabbar label[for=\"aus-t4\"]{background:#2d5a3d;color:#fff;border-color:#2d5a3d}\n#aus-t1:checked ~ .aus-panels .aus-p1,#aus-t2:checked ~ .aus-panels .aus-p2,#aus-t3:checked ~ .aus-panels .aus-p3,#aus-t4:checked ~ .aus-panels .aus-p4{display:block}\n.aus-nutrient-product .aus-tab-input:focus-visible + *{outline:none}\n.aus-nutrient-product table{width:100%;border-collapse:collapse;font-size:13px}\n.aus-nutrient-product th{background:#f6f8fb;padding:11px 12px;border-bottom:2px solid #d8e1e8;text-align:left;font-weight:bold;color:#15324b}\n.aus-nutrient-product td{padding:10px 12px;border-bottom:1px solid #e2e8f0;vertical-align:top}\n.aus-nutrient-product .aus-h3{color:#15324b;font-size:22px;margin:0 0 18px 0;padding-bottom:10px;border-bottom:3px solid #1d6fb8}\n.aus-nutrient-product .aus-box{background:#f8fafc;padding:20px;border-radius:16px;border-left:5px solid #1d6fb8;margin-bottom:20px}\n.aus-nutrient-product .aus-card{background:#fff;padding:20px;border-radius:16px;border:1px solid #d8e1e8;margin-bottom:20px}\n.aus-nutrient-product .aus-scroll{overflow-x:auto}\n.aus-nutrient-product details{background:#f8fafc;border:1px solid #d8e1e8;border-left:5px solid #1d6fb8;border-radius:12px;padding:14px 18px;margin-bottom:12px}\n.aus-nutrient-product summary{font-weight:600;color:#15324b;cursor:pointer}\n@media (max-width:860px){.aus-hero-grid,.aus-main-grid{grid-template-columns:1fr !important}.aus-guarantee{min-width:100% !important}.aus-nutrient-product .aus-tabbar label{flex:1 1 45%;text-align:center}}\n\u003c\/style\u003e\n\u003c!--\nProduct: Streptococcus Selection Broth (Pike)\nCatalogue No.: AS-1450\nManufacturer: AuSaMicS Pty Ltd, Melbourne, Australia\nCategory: Dehydrated culture medium — selective enrichment broth\nPrimary use: Selective enrichment of streptococci and enterococci from samples with heavy mixed flora\n--\u003e\n\u003carticle style=\"font-family: 'Segoe UI',system-ui,-apple-system,sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\" class=\"aus-nutrient-product\" lang=\"en-AU\"\u003e\u003cheader style=\"background: linear-gradient(135deg,#f0f4f8 0%,#d9e2ec 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #d8e1e8; border-left: 8px solid #2d5a3d;\"\u003e\n\u003cdiv class=\"aus-hero-grid\" style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #2d5a3d; font-weight: bold; margin-bottom: 12px; background: #fff; padding: 6px 12px; border-radius: 20px; border: 1px solid #d8e1e8;\"\u003eAuSaMicS Life Science • Selective Enrichment Media\u003c\/div\u003e\n\u003ch2 style=\"color: #15324b; margin: 0 0 8px 0; font-size: 32px; font-weight: bold; line-height: 1.25;\"\u003eStreptococcus Selection Broth (Pike)\u003c\/h2\u003e\n\u003cp style=\"color: #243b53; margin: 0 0 15px 0; font-size: 20px; opacity: .9;\"\u003eAzide–crystal violet broth for selective enrichment of streptococci and enterococci\u003c\/p\u003e\n\u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #334e68; max-width: 650px; font-weight: 500;\"\u003e\u003cstrong\u003eStreptococcus Selection Broth is a selective enrichment medium in which sodium azide, sodium sulfite and crystal violet suppress accompanying flora, so streptococci and enterococci can grow from heavily contaminated samples.\u003c\/strong\u003e It follows the formulation of Pike and is read by turbidity, followed by Gram stain and subculture.\u003c\/p\u003e\n\u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n\u003cspan style=\"background: #2d5a3d; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAS-1450\u003c\/span\u003e\u003cspan style=\"background: #fff; border: 2px solid #28a745; color: #28a745; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e30.6 g\/L\u003c\/span\u003e\u003cspan style=\"background: #28a745; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003epH 7.4 ± 0.2\u003c\/span\u003e\u003cspan style=\"background: #fff; border: 2px solid #2d5a3d; color: #2d5a3d; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eSelective Enrichment Broth\u003c\/span\u003e\u003cspan style=\"background: #c9a84c; color: #1a1a1a; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eAzide + Crystal Violet\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #4a5568;\"\u003e\n\u003cspan\u003e\u003cstrong\u003eReadout:\u003c\/strong\u003e Turbidity, then subculture\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eIncubation:\u003c\/strong\u003e 35–37 °C, 18–24 h\u003c\/span\u003e \u003cspan\u003e\u003cstrong\u003eYield:\u003c\/strong\u003e 500 g makes about 16 L\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size: 13px; color: #52606d; margin: 15px 0 0 0;\"\u003e\u003cstrong\u003eAlso known as:\u003c\/strong\u003e Streptococcus Selective Broth, Pike Broth, Enterococcus Selective Broth (azide–crystal violet formulation).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003caside class=\"aus-guarantee\" style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.1); min-width: 280px; border: 1px solid #d8e1e8;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003eAt a Glance\u003c\/h3\u003e\n\u003cdiv style=\"font-size: 14px; color: #4a5568; text-align: center; line-height: 1.5;\"\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eGram-negative inhibitors\u003c\/strong\u003e – Sodium azide, sodium sulfite\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eStaphylococcus inhibitor\u003c\/strong\u003e – Crystal violet\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eSterilisation\u003c\/strong\u003e – 118 °C, 15 min\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eNo esculin or bile\u003c\/strong\u003e – no colour reaction\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 10px;\"\u003e\n\u003cstrong\u003eResult type\u003c\/strong\u003e – Enrichment\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-top: 18px; padding: 15px; background: #f0f4f1; border-radius: 12px; text-align: center; border: 1px solid #c8d8cc;\"\u003e\n\u003cdiv style=\"color: #2d5a3d; font-weight: 600; font-size: 14px;\"\u003eLot-specific COA, TDS and SDS\u003c\/div\u003e\n\u003cdiv style=\"color: #4a5568; font-size: 13px; margin-top: 5px;\"\u003eAvailable for every lot\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\n\u003c\/header\u003e\u003cinput type=\"radio\" name=\"aus-tabs\" id=\"aus-t1\" class=\"aus-tab-input\" checked\u003e \u003cinput type=\"radio\" name=\"aus-tabs\" id=\"aus-t2\" class=\"aus-tab-input\"\u003e \u003cinput type=\"radio\" name=\"aus-tabs\" id=\"aus-t3\" class=\"aus-tab-input\"\u003e \u003cinput type=\"radio\" name=\"aus-tabs\" id=\"aus-t4\" class=\"aus-tab-input\"\u003e\u003cnav class=\"aus-tabbar\"\u003e\u003clabel for=\"aus-t1\"\u003eProduct Description\u003c\/label\u003e\u003clabel for=\"aus-t2\"\u003eTechnical Data\u003c\/label\u003e\u003clabel for=\"aus-t3\"\u003eQuality Control\u003c\/label\u003e\u003clabel for=\"aus-t4\"\u003eSafety \u0026amp; Storage\u003c\/label\u003e\u003c\/nav\u003e\n\u003cdiv class=\"aus-panels\"\u003e\n\u003csection class=\"aus-panel aus-p1\" id=\"description\"\u003e\n\u003cdiv class=\"aus-main-grid\" style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px;\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"aus-h3\"\u003ePrinciple of the Medium\u003c\/h3\u003e\n\u003cdiv class=\"aus-box\"\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #334e68;\"\u003eCasein and soy peptones, dextrose and L-cystine support the growth of streptococci and enterococci. Sodium azide and sodium sulfite inhibit Gram-negative rods, and a very low concentration of crystal violet suppresses staphylococci and many other Gram-positive bacteria. The broth enriches target organisms, which are then confirmed by Gram stain and subculture.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit,minmax(200px,1fr)); gap: 15px; margin-bottom: 30px;\"\u003e\n\u003cdiv style=\"background: #f0f4f1; padding: 15px; border-radius: 12px; border-left: 4px solid #2d5a3d;\"\u003e\n\u003cstrong style=\"color: #2d5a3d;\"\u003eAzide + Sulfite\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eSuppress Gram-negative rods\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff9e6; padding: 15px; border-radius: 12px; border-left: 4px solid #c9a84c;\"\u003e\n\u003cstrong style=\"color: #c9a84c;\"\u003eCrystal Violet\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eSuppresses staphylococci\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #e8f2ff; padding: 15px; border-radius: 12px; border-left: 4px solid #1565c0;\"\u003e\n\u003cstrong style=\"color: #1565c0;\"\u003eRich Base\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003ePeptones, dextrose and cystine for streptococci\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eHow It Compares\u003c\/h3\u003e\n\u003cdiv class=\"aus-scroll\" style=\"margin-bottom: 30px;\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eMedium\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eSelective system\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eReadout\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eMain use\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #f0f4f1;\"\u003e\n\u003ctd\u003e\u003cstrong\u003eStreptococcus Selection Broth (AS-1450)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eAzide, sulfite, crystal violet\u003c\/td\u003e\n\u003ctd\u003eTurbidity\u003c\/td\u003e\n\u003ctd\u003eEnrichment from mixed flora\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBile Esculin Agar (AS-1140)\u003c\/td\u003e\n\u003ctd\u003eOxgall\u003c\/td\u003e\n\u003ctd\u003eBlack zone (esculin)\u003c\/td\u003e\n\u003ctd\u003ePresumptive ID of enterococci\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBile Esculin Azide Broth, Enterococcosel-type (AS-1222)\u003c\/td\u003e\n\u003ctd\u003eOxgall + azide\u003c\/td\u003e\n\u003ctd\u003eBlackening (esculin)\u003c\/td\u003e\n\u003ctd\u003eRapid ID of suspected enterococci\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAzide Dextrose Broth\u003c\/td\u003e\n\u003ctd\u003eSodium azide\u003c\/td\u003e\n\u003ctd\u003eTurbidity\u003c\/td\u003e\n\u003ctd\u003eEnterococci in water (MPN)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eComparable Products\u003c\/h3\u003e\n\u003cdiv class=\"aus-scroll\" style=\"margin-bottom: 10px;\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eSupplier\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eProduct\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eRelationship\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #f0f4f1;\"\u003e\n\u003ctd\u003e\u003cstrong\u003eAuSaMicS\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eStreptococcus Selection Broth (Pike) (AS-1450)\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHiMedia\u003c\/td\u003e\n\u003ctd\u003eStreptococcus Selection Broth (M303)\u003c\/td\u003e\n\u003ctd\u003eSame published formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCondalab\u003c\/td\u003e\n\u003ctd\u003eEnterococcus Selective Broth (1204)\u003c\/td\u003e\n\u003ctd\u003eSame published formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size: 12px; color: #52606d; margin: 0 0 30px 0;\"\u003eBased on published formulations. Trademarks belong to their respective owners.\u003c\/p\u003e\n\u003ch3 class=\"aus-h3\"\u003eAdvantages and Limitations\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit,minmax(300px,1fr)); gap: 20px; margin-bottom: 30px;\"\u003e\n\u003cdiv style=\"background: #e8f5e8; padding: 20px; border-radius: 16px; border: 1px solid #c8e6c9;\"\u003e\n\u003ch4 style=\"color: #2e7d32; margin: 0 0 12px 0; font-size: 16px;\"\u003eAdvantages\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0; font-size: 14px; color: #2e7d32;\"\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e✓ \u003cstrong\u003eSuppresses heavy mixed flora\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e✓ \u003cstrong\u003eRecovers both streptococci and enterococci\u003c\/strong\u003e\n\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e✓ \u003cstrong\u003eSimple liquid enrichment\u003c\/strong\u003e before plating\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e✓ \u003cstrong\u003eLow autoclave temperature\u003c\/strong\u003e protects recovery\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff3e0; padding: 20px; border-radius: 16px; border: 1px solid #ffcc02;\"\u003e\n\u003ch4 style=\"color: #e65100; margin: 0 0 12px 0; font-size: 16px;\"\u003eLimitations\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0; font-size: 14px; color: #e65100;\"\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e▲ \u003cstrong\u003eNo differential reaction\u003c\/strong\u003e: confirm by subculture\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e▲ \u003cstrong\u003eSome Gram-negatives\u003c\/strong\u003e may grow partially\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e▲ \u003cstrong\u003eOverheating\u003c\/strong\u003e increases inhibition\u003c\/li\u003e\n\u003cli style=\"padding: 5px 0;\"\u003e▲ \u003cstrong\u003eContains sodium azide\u003c\/strong\u003e: careful disposal\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eFrequently Asked Questions\u003c\/h3\u003e\n\u003cdetails open=\"\"\u003e\n\u003csummary\u003eWhat is Streptococcus Selection Broth used for?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003eIt is a selective enrichment broth for streptococci and enterococci from samples with heavy mixed flora. Sodium azide and sodium sulfite suppress Gram-negative rods, and crystal violet suppresses staphylococci.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes Streptococcus Selection Broth show a colour or blackening reaction?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003eNo. It contains no esculin or bile, so growth is read as turbidity. Confirm organisms by Gram stain and subculture onto agar such as blood agar or Bile Esculin Agar.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy is it sterilised at 118 °C instead of 121 °C?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003eOverheating makes the medium more inhibitory and can reduce recovery of streptococci. Autoclave at 118 °C for 15 minutes, or use it the same day without autoclaving.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs this the same as BD Enterococcosel Broth?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003eNo. Enterococcosel Broth is a bile esculin azide broth that turns black with enterococci. This medium follows Pike’s azide–crystal violet formula and has no differential reaction.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat safety precautions apply to the sodium azide?\u003c\/summary\u003e\n\u003cp style=\"margin: 10px 0 0 0; font-size: 14px; color: #334e68;\"\u003eSodium azide can form explosive metal azides in copper or lead plumbing. Autoclave used media before disposal and flush drains with plenty of water; never pour unautoclaved media or concentrated solutions down drains.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/div\u003e\n\u003caside\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #d8e1e8; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #15324b; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003ctable style=\"font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\" style=\"width: 45%;\"\u003eCatalogue No.\u003c\/th\u003e\n\u003ctd\u003eAS-1450\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eUsage\u003c\/th\u003e\n\u003ctd\u003e30.6 g\/L\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eFinal pH\u003c\/th\u003e\n\u003ctd\u003e7.4 ± 0.2 at 25 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eSterilisation\u003c\/th\u003e\n\u003ctd\u003e118 °C, 15 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eIncubation\u003c\/th\u003e\n\u003ctd\u003e35–37 °C, 18–24 h, aerobic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003ePrepared medium\u003c\/th\u003e\n\u003ctd\u003eClear amber with a slight violet tint\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth scope=\"row\"\u003eHS code\u003c\/th\u003e\n\u003ctd\u003e3821.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #f0f4f1; padding: 20px; border-radius: 16px; border: 1px solid #c8d8cc; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #2d5a3d; margin: 0 0 12px 0; font-size: 16px; text-align: center;\"\u003eApplications\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0; font-size: 14px; color: #2d5a3d;\"\u003e\n\u003cli style=\"padding: 6px 0;\"\u003eEnrichment of streptococci from heavily contaminated samples\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003eEnrichment of enterococci before isolation on selective agar\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003eEnvironmental and research microbiology\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003eTeaching selective media principles\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff9e6; padding: 20px; border-radius: 16px; border: 1px solid #c9a84c; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #c9a84c; margin: 0 0 12px 0; font-size: 16px; text-align: center;\"\u003ePreparation\u003c\/h4\u003e\n\u003col style=\"list-style: none; padding: 0; margin: 0; font-size: 14px; color: #5d4037;\"\u003e\n\u003cli style=\"padding: 6px 0;\"\u003e\n\u003cstrong\u003e1.\u003c\/strong\u003e Suspend 30.6 g in 1 L of purified water.\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003e\n\u003cstrong\u003e2.\u003c\/strong\u003e Heat gently to dissolve completely.\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003e\n\u003cstrong\u003e3.\u003c\/strong\u003e Dispense into tubes (e.g. 10 mL).\u003c\/li\u003e\n\u003cli style=\"padding: 6px 0;\"\u003e\n\u003cstrong\u003e4.\u003c\/strong\u003e Autoclave at 118 °C for 15 minutes. Do not overheat.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #d8e1e8;\"\u003e\n\u003ch4 style=\"color: #15324b; margin: 0 0 12px 0; font-size: 16px; text-align: center;\"\u003eIntended Use\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eFor laboratory microbiological testing, quality control and research use only. Not intended for direct use in food, human or veterinary therapeutic applications, diagnosis, or consumption.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/aside\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"aus-panel aus-p2\" id=\"technical-data\"\u003e\n\u003ch3 class=\"aus-h3\"\u003eTypical Formula (g\/L)\u003c\/h3\u003e\n\u003cdiv class=\"aus-box\" style=\"padding: 0; overflow: hidden;\"\u003e\n\u003cdiv class=\"aus-scroll\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eIngredient\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eg\/L\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eFunction\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eCasein peptone\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e15.0\u003c\/td\u003e\n\u003ctd\u003eAmino acids and nitrogen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSoy peptone\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e5.0\u003c\/td\u003e\n\u003ctd\u003ePeptides, vitamins and carbohydrates\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDextrose\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e5.0\u003c\/td\u003e\n\u003ctd\u003eEnergy source\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium chloride\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e4.0\u003c\/td\u003e\n\u003ctd\u003eOsmotic balance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium citrate\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e1.0\u003c\/td\u003e\n\u003ctd\u003eBuffering and carbon source\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eL-Cystine\u003c\/td\u003e\n\u003ctd style=\"color: #2d5a3d; font-weight: 600;\"\u003e0.2\u003c\/td\u003e\n\u003ctd\u003eLowers redox potential; supports growth\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium sulfite\u003c\/td\u003e\n\u003ctd style=\"color: #b45309; font-weight: 600;\"\u003e0.2\u003c\/td\u003e\n\u003ctd\u003eReducing agent; inhibits Gram-negative rods\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSodium azide\u003c\/td\u003e\n\u003ctd style=\"color: #b45309; font-weight: 600;\"\u003e0.2\u003c\/td\u003e\n\u003ctd\u003eSelective agent against Gram-negative bacteria\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCrystal violet\u003c\/td\u003e\n\u003ctd style=\"color: #b45309; font-weight: 600;\"\u003e0.0002\u003c\/td\u003e\n\u003ctd\u003eSelective agent against staphylococci and other Gram-positives\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eTotal\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e30.6\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003eFinal pH 7.4 ± 0.2 at 25 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size: 12px; color: #52606d; margin: -10px 0 30px 0;\"\u003eFormula may be adjusted to meet performance requirements.\u003c\/p\u003e\n\u003ch3 class=\"aus-h3\"\u003eProcedure and Interpretation\u003c\/h3\u003e\n\u003cdiv class=\"aus-card\"\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 14px; color: #334e68;\"\u003e\u003cstrong\u003eInoculate\u003c\/strong\u003e the sample or swab into the broth and incubate aerobically at 35–37 °C for 18–24 hours.\u003c\/p\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 14px; color: #334e68;\"\u003e\u003cstrong\u003ePositive:\u003c\/strong\u003e turbidity. Gram-stain the broth and subculture onto blood agar or Bile Esculin Agar for identification.\u003c\/p\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #334e68;\"\u003e\u003cstrong\u003eNegative:\u003c\/strong\u003e no turbidity after 24 hours.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eReferences\u003c\/h3\u003e\n\u003col style=\"font-size: 13px; color: #4a5568; padding-left: 20px; margin: 0;\"\u003e\n\u003cli style=\"padding: 4px 0;\"\u003ePike RM. An enrichment broth for isolating hemolytic streptococci from throat swabs. \u003cem\u003eProc Soc Exp Biol Med\u003c\/em\u003e. 1944;57:186–187.\u003c\/li\u003e\n\u003cli style=\"padding: 4px 0;\"\u003eHiMedia Laboratories. Technical Data: Streptococcus Selection Broth (M303).\u003c\/li\u003e\n\u003cli style=\"padding: 4px 0;\"\u003eMacFaddin JF. \u003cem\u003eMedia for Isolation–Cultivation–Identification–Maintenance of Medical Bacteria\u003c\/em\u003e, Vol. 1. Williams \u0026amp; Wilkins; 1985.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/section\u003e\n\u003csection class=\"aus-panel aus-p3\" id=\"quality-control\"\u003e\n\u003ch3 class=\"aus-h3\"\u003eQuality Control\u003c\/h3\u003e\n\u003cdiv class=\"aus-scroll aus-card\" style=\"padding: 0; overflow: hidden;\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eParameter\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (powder)\u003c\/td\u003e\n\u003ctd\u003eBeige, free-flowing, homogeneous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePrepared medium\u003c\/td\u003e\n\u003ctd\u003eClear amber with a slight violet tint\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH at 25 °C\u003c\/td\u003e\n\u003ctd\u003e7.4 ± 0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eRecommended Control Strains (35–37 °C, 18–24 h)\u003c\/h3\u003e\n\u003cdiv class=\"aus-scroll aus-card\" style=\"padding: 0; overflow: hidden;\"\u003e\n\u003ctable\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth scope=\"col\"\u003eOrganism\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eExpected result\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eStreptococcus pyogenes\u003c\/em\u003e ATCC 19615\u003c\/td\u003e\n\u003ctd\u003e50–100\u003c\/td\u003e\n\u003ctd\u003eGood growth (turbidity)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eEnterococcus faecalis\u003c\/em\u003e ATCC 29212 (WDCM 00087)\u003c\/td\u003e\n\u003ctd\u003e50–100\u003c\/td\u003e\n\u003ctd\u003eGood growth (turbidity)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eEscherichia coli\u003c\/em\u003e ATCC 25922 (WDCM 00013)\u003c\/td\u003e\n\u003ctd\u003e≥ 10³\u003c\/td\u003e\n\u003ctd\u003eInhibited\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eStaphylococcus aureus\u003c\/em\u003e ATCC 25923 (WDCM 00034)\u003c\/td\u003e\n\u003ctd\u003e≥ 10³\u003c\/td\u003e\n\u003ctd\u003eInhibited to partially inhibited\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: linear-gradient(135deg,#f0f4f1 0%,#c8d8cc 100%); padding: 20px; border-radius: 16px; border-left: 5px solid #2d5a3d;\"\u003e\n\u003cstrong style=\"color: #2d5a3d;\"\u003eBatch release:\u003c\/strong\u003e Each lot is tested for appearance, pH and growth promotion. Results are reported on the lot Certificate of Analysis.\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"aus-panel aus-p4\" id=\"safety-storage\"\u003e\n\u003ch3 class=\"aus-h3\"\u003eSafety\u003c\/h3\u003e\n\u003cdiv class=\"aus-card\"\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #334e68;\"\u003eThe powder contains 0.65% sodium azide and is not classified as hazardous under the GHS at this concentration. Sodium azide can form explosive metal azides in copper or lead plumbing and releases toxic gas with acids. Autoclave used media before disposal and flush drains with plenty of water. Treat inoculated media as potentially infectious. The full SDS is available for every lot.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3 class=\"aus-h3\"\u003eStorage\u003c\/h3\u003e\n\u003cdiv class=\"aus-card\"\u003e\n\u003cp style=\"margin: 0 0 8px 0; font-size: 14px; color: #334e68;\"\u003e\u003cstrong\u003eDehydrated medium:\u003c\/strong\u003e Store at 10–30 °C in a dry place, protected from light, with the container tightly closed.\u003c\/p\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #334e68;\"\u003e\u003cstrong\u003ePrepared medium:\u003c\/strong\u003e Store tubes at 2–8 °C, protected from light. Validate the use period in your laboratory.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003c\/div\u003e\n\u003cfooter style=\"margin-top: 30px; background: #f8fafc; border: 1px solid #d8e1e8; border-radius: 16px; padding: 20px; font-size: 13px; color: #52606d;\"\u003e\u003cstrong\u003eAuSaMicS Life Science:\u003c\/strong\u003e Dehydrated culture media and laboratory reagents manufactured in Melbourne, Australia. For laboratory microbiological testing, quality control and research use only. Not intended for direct use in food, human or veterinary therapeutic applications, diagnosis, or consumption. Technical content last reviewed: October 2026.\u003c\/footer\u003e\u003cscript type=\"application\/ld+json\"\u003e\n{\n \"@context\": \"https:\/\/schema.org\",\n \"@type\": \"FAQPage\",\n \"mainEntity\": [\n  {\n   \"@type\": \"Question\",\n   \"name\": \"What is Streptococcus Selection Broth used for?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"It is a selective enrichment broth for streptococci and enterococci from samples with heavy mixed flora. Sodium azide and sodium sulfite suppress Gram-negative rods, and crystal violet suppresses staphylococci.\"\n   }\n  },\n  {\n   \"@type\": \"Question\",\n   \"name\": \"Does Streptococcus Selection Broth show a colour or blackening reaction?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"No. It contains no esculin or bile, so growth is read as turbidity. Confirm organisms by Gram stain and subculture onto agar such as blood agar or Bile Esculin Agar.\"\n   }\n  },\n  {\n   \"@type\": \"Question\",\n   \"name\": \"Why is it sterilised at 118 °C instead of 121 °C?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"Overheating makes the medium more inhibitory and can reduce recovery of streptococci. Autoclave at 118 °C for 15 minutes, or use it the same day without autoclaving.\"\n   }\n  },\n  {\n   \"@type\": \"Question\",\n   \"name\": \"Is this the same as BD Enterococcosel Broth?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"No. Enterococcosel Broth is a bile esculin azide broth that turns black with enterococci. This medium follows Pike’s azide–crystal violet formula and has no differential reaction.\"\n   }\n  },\n  {\n   \"@type\": \"Question\",\n   \"name\": \"What safety precautions apply to the sodium azide?\",\n   \"acceptedAnswer\": {\n    \"@type\": \"Answer\",\n    \"text\": \"Sodium azide can form explosive metal azides in copper or lead plumbing. Autoclave used media before disposal and flush drains with plenty of water; never pour unautoclaved media or concentrated solutions down drains.\"\n   }\n  }\n ]\n}\n  \u003c\/script\u003e\u003c\/article\u003e","brand":"AuSaMicS","offers":[{"title":"500 g","offer_id":50608698753279,"sku":"AS-1450-500G","price":150.0,"currency_code":"AUD","in_stock":true}],"url":"https:\/\/ausamics.com.au\/products\/streptococcus-selection-broth-pike-as-1450","provider":"AuSaMicS","version":"1.0","type":"link"}