{"product_id":"terrific-broth-tb-high-yield-bacterial-media-ausamics","title":"Terrific Broth (TB) | High-Yield Bacterial Media | AuSaMicS","description":"\u003c!-- ============================================================\n     AuSaMicS — Terrific Broth (TB)\n     Catalog: AS-1429 | Shopify Product Page HTML Embed\n     Version: 1.0 | ausamics.com.au\n     SEO + LLMo + DuckDuckGo + JSON-LD Optimised\n     ============================================================ --\u003e\n\u003cdiv class=\"aus-tb\"\u003e\n\u003cdiv class=\"aus-hero\"\u003e\n\u003cdiv class=\"aus-hero-top\"\u003e\n\u003cdiv\u003e\n\u003ch1\u003eTerrific Broth (TB)\u003c\/h1\u003e\n\u003cp\u003eHigh-yield, phosphate-buffered enriched medium for maximum biomass, plasmid DNA yield, and recombinant protein expression in \u003cem\u003eE. coli\u003c\/em\u003e and related hosts. Manufactured in Australia with full batch documentation.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"aus-cat-badge\"\u003eAS-1429\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- TABS --\u003e\n\u003cdiv class=\"aus-tabs\"\u003e\n\u003cbutton class=\"aus-tab active\"\u003eProduct Description\u003c\/button\u003e \u003cbutton class=\"aus-tab\"\u003eTechnical Data Sheet\u003c\/button\u003e \u003cbutton class=\"aus-tab\"\u003eSafety Data Sheet\u003c\/button\u003e \u003cbutton class=\"aus-tab\"\u003eCertificate of Analysis\u003c\/button\u003e\n\u003c\/div\u003e\n\u003c!-- ================================================================ TAB 1: PRODUCT DESCRIPTION --\u003e\n\u003cdiv id=\"tb-pd\" class=\"aus-panel active\"\u003e\n\u003cdiv class=\"quick-specs\"\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eCatalog No.\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003eAS-1429\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eForm\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003eDehydrated powder\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eFinal pH\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003e7.2 ± 0.2 at 25 °C\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eBuffering\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003ePotassium phosphate 0.089 M\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003ePackaging\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003e100 g – 5 kg\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eHS Code\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003e3821.00\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- OVERVIEW --\u003e\n\u003cp class=\"sec-title\"\u003eOverview\u003c\/p\u003e\n\u003cp style=\"font-size: 14px; color: #374151; margin-bottom: 16px; line-height: 1.7;\"\u003eTerrific Broth (TB) is an enriched, phosphate-buffered bacterial growth medium specifically formulated for high-density \u003cem\u003eE. coli\u003c\/em\u003e cultivation. Developed by Tartof \u0026amp; Hobbs (1987), TB contains nearly double the tryptone and four times the yeast extract of standard LB Broth, combined with glycerol as a supplementary carbon source and a potassium phosphate buffer system that prevents the catastrophic pH drops that terminate growth in unbuffered media during high-density fermentation.\u003c\/p\u003e\n\u003cp style=\"font-size: 14px; color: #374151; margin-bottom: 28px; line-height: 1.7;\"\u003eThe result is consistently higher biomass yields, significantly greater plasmid DNA quantities per litre of culture, and more robust recombinant protein expression compared to LB or 2×YT. AuSaMicS AS-1429 is manufactured to microbiological grade standards and dispatched same-week from Melbourne with full batch documentation.\u003c\/p\u003e\n\u003c!-- INGREDIENTS TABLE --\u003e\n\u003cp class=\"sec-title\"\u003eComposition (per litre of prepared medium)\u003c\/p\u003e\n\u003ctable class=\"ing-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredient\u003c\/th\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003cth\u003eFunction\u003c\/th\u003e\n\u003cth\u003eMechanism\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eTryptone (Casein Digest)\u003c\/td\u003e\n\u003ctd\u003e12.0 g\/L\u003c\/td\u003e\n\u003ctd\u003ePrimary nitrogen \u0026amp; amino acid source\u003c\/td\u003e\n\u003ctd\u003ePancreatic digest of casein providing free amino acids and peptides for rapid protein biosynthesis; 2× the concentration of LB Broth — sustains protein synthesis at high cell densities without nitrogen limitation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eYeast Extract\u003c\/td\u003e\n\u003ctd\u003e24.0 g\/L\u003c\/td\u003e\n\u003ctd\u003eVitamins, cofactors \u0026amp; growth factors\u003c\/td\u003e\n\u003ctd\u003eAutolysate providing B-vitamins, nucleotide bases, trace minerals, and growth factors; 4× the concentration of LB — prevents vitamin limitation at OD₆₀₀ \u0026gt;10; critical for sustained plasmid replication and expression machinery function\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGlycerol\u003c\/td\u003e\n\u003ctd\u003e0.4% (v\/v)\u003c\/td\u003e\n\u003ctd\u003eNon-fermentable supplementary carbon source\u003c\/td\u003e\n\u003ctd\u003eSlowly metabolised via gluconeogenesis without acidic fermentation by-products; prolongs exponential growth phase by supplementing glucose-free carbon; does not trigger catabolite repression — maintains cAMP-CRP regulated gene expression\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePotassium dihydrogen phosphate (KH₂PO₄)\u003c\/td\u003e\n\u003ctd\u003e0.089 M component\u003c\/td\u003e\n\u003ctd\u003eBuffer — pH stabilisation (acidic arm)\u003c\/td\u003e\n\u003ctd\u003eConjugate acid of the phosphate buffer pair; maintains medium pH in the 7.0–7.4 range throughout high-density growth by absorbing protons generated during organic acid metabolism and glucose fermentation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDipotassium hydrogen phosphate (K₂HPO₄)\u003c\/td\u003e\n\u003ctd\u003e0.089 M component\u003c\/td\u003e\n\u003ctd\u003eBuffer — pH stabilisation (basic arm) \u0026amp; K⁺ source\u003c\/td\u003e\n\u003ctd\u003eConjugate base of phosphate pair; donates protons to neutralise base accumulation; provides K⁺ ions for membrane potential and active transport; total phosphate 0.089 M — sufficient buffering capacity for cultures reaching OD₆₀₀ \u0026gt;15\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd colspan=\"4\"\u003e\n\u003cstrong\u003eFinal pH: 7.2 ± 0.2 at 25 °C\u003c\/strong\u003e — Slightly alkaline optimum for phosphate-buffered high-density \u003cem\u003eE. coli\u003c\/em\u003e cultivation; phosphate buffer maintains this range throughout the growth cycle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c!-- APPLICATIONS --\u003e\n\u003cp class=\"sec-title\"\u003eApplications\u003c\/p\u003e\n\u003cdiv class=\"apps-grid\"\u003e\n\u003cdiv class=\"app-item\"\u003e\n\u003cdiv class=\"app-icon\"\u003eP\u003c\/div\u003e\n\u003cdiv class=\"app-text\"\u003e\n\u003cstrong\u003ePlasmid DNA production\u003c\/strong\u003e\u003cspan\u003eMaximum yield of high-copy plasmid DNA per litre — miniprep, midiprep, and maxiprep workflows\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"app-item\"\u003e\n\u003cdiv class=\"app-icon\"\u003eR\u003c\/div\u003e\n\u003cdiv class=\"app-text\"\u003e\n\u003cstrong\u003eRecombinant protein expression\u003c\/strong\u003e\u003cspan\u003eHigh-density culture for inducible expression systems (IPTG, arabinose) — greater biomass per litre means more protein per batch\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"app-item\"\u003e\n\u003cdiv class=\"app-icon\"\u003eH\u003c\/div\u003e\n\u003cdiv class=\"app-text\"\u003e\n\u003cstrong\u003eHigh-density fermentation\u003c\/strong\u003e\u003cspan\u003eShake-flask and bioreactor cultures targeting OD₆₀₀ \u0026gt;10 — phosphate buffer prevents pH crash at high cell densities\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"app-item\"\u003e\n\u003cdiv class=\"app-icon\"\u003eC\u003c\/div\u003e\n\u003cdiv class=\"app-text\"\u003e\n\u003cstrong\u003eCompetent cell preparation\u003c\/strong\u003e\u003cspan\u003ePre-transformation high-density culture where maximum viable cell count is needed for electroporation or chemical competency protocols\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"app-item\"\u003e\n\u003cdiv class=\"app-icon\"\u003eE\u003c\/div\u003e\n\u003cdiv class=\"app-text\"\u003e\n\u003cstrong\u003eEnzyme production\u003c\/strong\u003e\u003cspan\u003eHigh-yield cultivation for restriction enzymes, ligases, polymerases, and other recombinant enzyme expression systems\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"app-item\"\u003e\n\u003cdiv class=\"app-icon\"\u003eA\u003c\/div\u003e\n\u003cdiv class=\"app-text\"\u003e\n\u003cstrong\u003eAntibody fragment expression\u003c\/strong\u003e\u003cspan\u003eFab, scFv, and nanobody production in \u003cem\u003eE. coli\u003c\/em\u003e periplasmic and cytoplasmic expression systems\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- PROS \/ CONS --\u003e\n\u003cp class=\"sec-title\"\u003eStrengths \u0026amp; Limitations\u003c\/p\u003e\n\u003cdiv class=\"pros-cons\"\u003e\n\u003cdiv class=\"pc-box pc-pro\"\u003e\n\u003cdiv class=\"pc-title\"\u003eStrengths\u003c\/div\u003e\n\u003cdiv class=\"pc-item\"\u003e\n\u003cspan class=\"pc-dot\"\u003e\u003c\/span\u003e\u003cspan\u003ePhosphate buffering — prevents pH crash in dense cultures, extending exponential growth phase significantly beyond unbuffered media\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pc-item\"\u003e\n\u003cspan class=\"pc-dot\"\u003e\u003c\/span\u003e\u003cspan\u003eConsistently yields 2–5× more plasmid DNA per litre than LB Broth\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pc-item\"\u003e\n\u003cspan class=\"pc-dot\"\u003e\u003c\/span\u003e\u003cspan\u003eGlycerol carbon source avoids acidic fermentation by-products that inhibit growth and protein folding\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pc-item\"\u003e\n\u003cspan class=\"pc-dot\"\u003e\u003c\/span\u003e\u003cspan\u003eHigh yeast extract prevents vitamin limitation at OD₆₀₀ \u0026gt;10\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pc-item\"\u003e\n\u003cspan class=\"pc-dot\"\u003e\u003c\/span\u003e\u003cspan\u003eCompatible with all standard \u003cem\u003eE. coli\u003c\/em\u003e expression strains (BL21, BL21(DE3), Rosetta, HMS174)\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pc-item\"\u003e\n\u003cspan class=\"pc-dot\"\u003e\u003c\/span\u003e\u003cspan\u003eFull COA, TDS, SDS per batch — audit-ready documentation\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pc-box pc-con\"\u003e\n\u003cdiv class=\"pc-title\"\u003eLimitations\u003c\/div\u003e\n\u003cdiv class=\"pc-item\"\u003e\n\u003cspan class=\"pc-dot\"\u003e\u003c\/span\u003e\u003cspan\u003eNot suitable for transformation recovery — use SOC Medium (AS-1428) post-transformation\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pc-item\"\u003e\n\u003cspan class=\"pc-dot\"\u003e\u003c\/span\u003e\u003cspan\u003eHigher cost per litre than LB — not recommended for routine maintenance cultures\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pc-item\"\u003e\n\u003cspan class=\"pc-dot\"\u003e\u003c\/span\u003e\u003cspan\u003eHigh nutrient content may support faster contaminant growth — strict aseptic technique essential\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pc-item\"\u003e\n\u003cspan class=\"pc-dot\"\u003e\u003c\/span\u003e\u003cspan\u003ePhosphate can precipitate with divalent cations (Mg²⁺, Ca²⁺) — do not supplement with MgSO₄ or CaCl₂ directly\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pc-item\"\u003e\n\u003cspan class=\"pc-dot\"\u003e\u003c\/span\u003e\u003cspan\u003eNot suitable for yeast or fungal cultures — use PDB (AS-1331) or MEB instead\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- COMPARATIVE MEDIA TABLE --\u003e\n\u003cp class=\"sec-title\"\u003eComparative Media — High-Density Bacterial Culture \u0026amp; Protein Expression\u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable class=\"cmp-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eMedium\u003c\/th\u003e\n\u003cth\u003eTryptone (g\/L)\u003c\/th\u003e\n\u003cth\u003eYeast Extract (g\/L)\u003c\/th\u003e\n\u003cth\u003eBuffered\u003c\/th\u003e\n\u003cth\u003eCarbon source\u003c\/th\u003e\n\u003cth\u003eMax OD₆₀₀\u003c\/th\u003e\n\u003cth\u003ePrimary use\u003c\/th\u003e\n\u003cth\u003eAuSaMicS Cat.\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr class=\"hl\"\u003e\n\u003ctd\u003eTerrific Broth (TB) — AS-1429\u003c\/td\u003e\n\u003ctd\u003e12.0\u003c\/td\u003e\n\u003ctd\u003e24.0\u003c\/td\u003e\n\u003ctd\u003eYes (phosphate)\u003c\/td\u003e\n\u003ctd\u003eGlycerol 0.4%\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;20\u003c\/td\u003e\n\u003ctd\u003eHigh-density culture, plasmid \u0026amp; protein production\u003c\/td\u003e\n\u003ctd\u003eAS-1429\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLB Broth (Lennox)\u003c\/td\u003e\n\u003ctd\u003e10.0\u003c\/td\u003e\n\u003ctd\u003e5.0\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003ctd\u003eNone\u003c\/td\u003e\n\u003ctd\u003e3–5\u003c\/td\u003e\n\u003ctd\u003eRoutine \u003cem\u003eE. coli\u003c\/em\u003e culture, cloning\u003c\/td\u003e\n\u003ctd\u003eAS-1271\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2×YT Broth\u003c\/td\u003e\n\u003ctd\u003e16.0\u003c\/td\u003e\n\u003ctd\u003e10.0\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003ctd\u003eNone\u003c\/td\u003e\n\u003ctd\u003e8–10\u003c\/td\u003e\n\u003ctd\u003ePhage display, antibody libraries\u003c\/td\u003e\n\u003ctd\u003eAS-1400\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSOC Medium\u003c\/td\u003e\n\u003ctd\u003e20.0 (casein)\u003c\/td\u003e\n\u003ctd\u003e5.0\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003ctd\u003eGlucose (post-autoclave)\u003c\/td\u003e\n\u003ctd\u003e3–5\u003c\/td\u003e\n\u003ctd\u003ePost-transformation recovery only\u003c\/td\u003e\n\u003ctd\u003eAS-1428\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAuto-Induction Medium\u003c\/td\u003e\n\u003ctd\u003e12.0\u003c\/td\u003e\n\u003ctd\u003e24.0\u003c\/td\u003e\n\u003ctd\u003eYes (phosphate)\u003c\/td\u003e\n\u003ctd\u003eGlucose + lactose\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;20\u003c\/td\u003e\n\u003ctd\u003eAuto-induction protein expression (no IPTG needed)\u003c\/td\u003e\n\u003ctd\u003eAS-1430\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eNutrient Broth\u003c\/td\u003e\n\u003ctd\u003e5.0 (peptone)\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003ctd\u003eNo\u003c\/td\u003e\n\u003ctd\u003eNone\u003c\/td\u003e\n\u003ctd\u003e2–3\u003c\/td\u003e\n\u003ctd\u003eGeneral bacteriology, maintenance\u003c\/td\u003e\n\u003ctd\u003eAS-1310\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c!-- CROSS-REFERENCE --\u003e\n\u003cp class=\"sec-title\"\u003eCross-Reference \/ Equivalent Products\u003c\/p\u003e\n\u003cp style=\"font-size: 13px; color: #6b7280; margin-bottom: 12px;\"\u003eAS-1429 is equivalent to the following products from major international suppliers:\u003c\/p\u003e\n\u003ctable class=\"aus-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eSupplier\u003c\/th\u003e\n\u003cth\u003eProduct Name\u003c\/th\u003e\n\u003cth\u003eCatalogue Number\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eOxoid (Thermo Fisher)\u003c\/td\u003e\n\u003ctd\u003eTerrific Broth\u003c\/td\u003e\n\u003ctd\u003eCM0955\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBD Difco\u003c\/td\u003e\n\u003ctd\u003eTerrific Broth\u003c\/td\u003e\n\u003ctd\u003e243820\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMerck (Sigma-Aldrich)\u003c\/td\u003e\n\u003ctd\u003eTerrific Broth\u003c\/td\u003e\n\u003ctd\u003eT9179\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eThermo Fisher Scientific\u003c\/td\u003e\n\u003ctd\u003eTerrific Broth Modified\u003c\/td\u003e\n\u003ctd\u003eA1374401\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\u003cstrong\u003eAuSaMicS\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eTerrific Broth (TB)\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003eAS-1429\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c!-- PREPARATION --\u003e\n\u003cp class=\"sec-title\"\u003ePreparation Instructions\u003c\/p\u003e\n\u003cdiv class=\"prep-box\"\u003e\n\u003cstrong\u003e1. Weigh:\u003c\/strong\u003e Suspend the required amount of AS-1429 dehydrated powder in distilled or deionised water per the label reconstitution rate.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e2. Mix:\u003c\/strong\u003e Stir to dissolve completely. The broth will appear slightly turbid and golden-yellow due to the high yeast extract content.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e3. Autoclave:\u003c\/strong\u003e Sterilise at \u003cstrong\u003e121 °C for 15 minutes\u003c\/strong\u003e (15 psi). Allow to cool before use.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e4. Dispense:\u003c\/strong\u003e Distribute into sterile flasks (fill to maximum 20–25% of flask volume to ensure adequate aeration in shake-flask culture).\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003e5. Inoculate:\u003c\/strong\u003e Inoculate with a single colony or overnight starter culture (1:50–1:100 dilution). Incubate at 37 °C with shaking at 200–250 rpm.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage of prepared TB:\u003c\/strong\u003e Store at 2–8 °C for up to 4 weeks. Discard if turbidity, precipitation, or colour change is observed prior to inoculation.\u003cbr\u003e\u003cbr\u003e\n\u003cdiv class=\"prep-tip\"\u003e💡 \u003cstrong\u003ePro tip — for inducible expression:\u003c\/strong\u003e Grow culture to OD₆₀₀ 0.6–0.8 before adding IPTG (typically 0.1–1.0 mM). The phosphate buffer allows continued growth post-induction, maximising protein accumulation. Lower induction temperatures (16–25 °C) improve soluble protein yield for difficult-to-fold targets.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- FAQ --\u003e\n\u003cp class=\"sec-title\"\u003eFrequently Asked Questions\u003c\/p\u003e\n\u003cdiv style=\"margin-bottom: 28px;\"\u003e\n\u003cdiv class=\"faq-item\"\u003e\n\u003cdiv class=\"faq-q\"\u003eQ1: Why does Terrific Broth produce more plasmid DNA than LB?\u003c\/div\u003e\n\u003cdiv class=\"faq-a\"\u003eTB contains 4× the yeast extract and 2× the tryptone of LB, providing a richer nutrient supply that supports higher cell densities (OD₆₀₀ \u0026gt;20 vs 3–5 in LB). More cells per litre means more plasmid DNA per preparation. The phosphate buffer also prevents the pH drop that would otherwise terminate growth mid-exponential phase in LB cultures at high density, allowing cultures to reach their maximum cell yield.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq-item\"\u003e\n\u003cdiv class=\"faq-q\"\u003eQ2: Why is glycerol used instead of glucose in Terrific Broth?\u003c\/div\u003e\n\u003cdiv class=\"faq-a\"\u003eGlycerol is metabolised slowly via gluconeogenesis without generating the acidic fermentation by-products (acetate, lactate) that accumulate when glucose is present in rich media at high cell densities. These organic acids inhibit growth, destabilise plasmids, and impair protein folding. Glycerol provides a clean carbon source that extends the exponential growth phase while the phosphate buffer handles any residual pH changes.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq-item\"\u003e\n\u003cdiv class=\"faq-q\"\u003eQ3: Can Terrific Broth be used for transformation recovery?\u003c\/div\u003e\n\u003cdiv class=\"faq-a\"\u003eNo — TB is not recommended for post-transformation recovery. The high nutrient content and phosphate buffer are optimised for dense, growing cultures rather than the delicate recovery phase immediately after heat shock or electroporation. Use SOC Medium (AS-1428) for transformation recovery, then transfer cells to TB for expression or plasmid production cultures.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq-item\"\u003e\n\u003cdiv class=\"faq-q\"\u003eQ4: Which E. coli strains are recommended for use with Terrific Broth?\u003c\/div\u003e\n\u003cdiv class=\"faq-a\"\u003eTB is compatible with all standard laboratory and expression strains including BL21(DE3), BL21(DE3)pLysS, Rosetta, HMS174(DE3), C41(DE3), C43(DE3), and DH5α. It is particularly recommended for strains used in IPTG-inducible T7 promoter expression systems where high biomass prior to induction is critical for maximal protein yield.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"faq-item\"\u003e\n\u003cdiv class=\"faq-q\"\u003eQ5: Can I add glucose or MgSO₄ to Terrific Broth?\u003c\/div\u003e\n\u003cdiv class=\"faq-a\"\u003eGlucose addition is not recommended — it triggers catabolite repression and generates acetate at high cell densities, counteracting the benefits of the phosphate buffer. MgSO₄ and CaCl₂ should not be added directly to TB as phosphate ions precipitate with divalent cations, forming insoluble complexes that deplete the buffer capacity and reduce nutrient availability.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"disclaimer\"\u003e\n\u003cstrong\u003eImportant Notice:\u003c\/strong\u003e For laboratory and research use only. Not for human consumption, veterinary, food, or household use. By purchasing, the buyer confirms compliance with all applicable Australian laws, institutional biosafety requirements, and workplace health and safety regulations. AuSaMicS Pty Ltd accepts no liability for misuse outside a controlled laboratory environment.\u003c\/div\u003e\n\u003cdiv class=\"intent-queries\"\u003eTerrific Broth Australia | TB medium molecular biology supplier Melbourne | buy Terrific Broth E. coli high density culture | plasmid DNA production media Australia | AS-1429 AuSaMicS | high yield bacterial growth media | recombinant protein expression broth Australia | phosphate buffered bacterial media | high density E. coli culture media | Terrific Broth vs LB plasmid yield | BL21 expression media Australia | IPTG induction media high yield\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- \/tb-pd --\u003e \u003c!-- ================================================================ TAB 2: TDS --\u003e\n\u003cdiv id=\"tb-tds\" class=\"aus-panel\"\u003e\n\u003cp class=\"sec-title\"\u003eTechnical Data Sheet — AS-1429 Terrific Broth\u003c\/p\u003e\n\u003cdiv class=\"quick-specs\"\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eDocument Type\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003eTechnical Data Sheet\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eProduct\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003eTerrific Broth (TB)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eCatalog No.\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003eAS-1429\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eRevision\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003e1.0\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eIssuer\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003eAuSaMicS Pty Ltd\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp class=\"sec-title\"\u003eTechnical Overview \u0026amp; Biochemistry\u003c\/p\u003e\n\u003cp style=\"font-size: 14px; color: #374151; margin-bottom: 16px; line-height: 1.7;\"\u003eTerrific Broth was developed by Tartof \u0026amp; Hobbs (1987) to overcome the growth limitations of LB Broth in high-density bacterial cultivation. The two principal innovations are: (1) a greatly enriched nutrient matrix — 12 g\/L tryptone and 24 g\/L yeast extract — that eliminates amino acid and vitamin limitation at high cell densities; and (2) a potassium phosphate buffer system that maintains medium pH in the physiological range (7.0–7.4) throughout the growth cycle, preventing the acid accumulation that terminates exponential growth in LB at OD₆₀₀ above 3–4.\u003c\/p\u003e\n\u003cp style=\"font-size: 14px; color: #374151; margin-bottom: 28px; line-height: 1.7;\"\u003eThe inclusion of glycerol (0.4% v\/v) as a supplementary carbon source provides a slowly metabolised, non-acidifying substrate that extends the exponential growth phase without triggering catabolite repression or generating inhibitory organic acids. Together these features enable TB cultures to routinely reach OD₆₀₀ \u0026gt;20 in standard shake flasks — delivering 5–10× more viable cells per litre than LB and proportionally higher yields of plasmid DNA, recombinant protein, and other biotechnological products.\u003c\/p\u003e\n\u003cp class=\"sec-title\"\u003ePhysical \u0026amp; Chemical Properties\u003c\/p\u003e\n\u003ctable class=\"aus-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (dehydrated powder)\u003c\/td\u003e\n\u003ctd\u003eCream to golden-yellow, homogeneous, free-flowing powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (prepared broth)\u003c\/td\u003e\n\u003ctd\u003eGolden-yellow to amber, slightly turbid solution (normal for high yeast extract content)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH (prepared medium at 25 °C)\u003c\/td\u003e\n\u003ctd\u003e7.2 ± 0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer system\u003c\/td\u003e\n\u003ctd\u003ePotassium phosphate (KH₂PO₄ \/ K₂HPO₄) at 0.089 M total concentration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffering capacity (pH range)\u003c\/td\u003e\n\u003ctd\u003eEffective pH 6.8–7.6 throughout high-density growth\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSterilisation\u003c\/td\u003e\n\u003ctd\u003eAutoclave at 121 °C, 15 min (15 psi)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMoisture content (powder)\u003c\/td\u003e\n\u003ctd\u003e≤6%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTypical max OD₆₀₀ (shake flask, 37 °C)\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;20 (vs 3–5 for LB)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHS Tariff Code\u003c\/td\u003e\n\u003ctd\u003e3821.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"sec-title\"\u003eDetailed Composition (per litre)\u003c\/p\u003e\n\u003ctable class=\"ing-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eIngredient\u003c\/th\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003cth\u003eFunction\u003c\/th\u003e\n\u003cth\u003eMechanism\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eTryptone (Casein Digest)\u003c\/td\u003e\n\u003ctd\u003e12.0 g\/L\u003c\/td\u003e\n\u003ctd\u003eAmino acid \u0026amp; nitrogen source\u003c\/td\u003e\n\u003ctd\u003ePancreatic digest providing free amino acids; 2× LB concentration sustains protein synthesis at high cell densities without nitrogen limitation; rich in tryptophan (unlike soy peptone) — important for tryptophan-regulated expression systems\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eYeast Extract\u003c\/td\u003e\n\u003ctd\u003e24.0 g\/L\u003c\/td\u003e\n\u003ctd\u003eVitamins, cofactors \u0026amp; nucleotide precursors\u003c\/td\u003e\n\u003ctd\u003e4× LB concentration; prevents B-vitamin depletion at OD₆₀₀ \u0026gt;10; provides purine\/pyrimidine bases reducing nucleotide synthesis burden; trace minerals support enzyme cofactor requirements at high cell density\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGlycerol\u003c\/td\u003e\n\u003ctd\u003e0.4% (v\/v) ≈ 3.2 g\/L\u003c\/td\u003e\n\u003ctd\u003eSupplementary carbon source\u003c\/td\u003e\n\u003ctd\u003eMetabolised via glycerol-3-phosphate through glycolysis; does not trigger catabolite repression (no cAMP suppression); no acidic fermentation by-products; provides energy for sustained growth after tryptone carbon is exhausted\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eKH₂PO₄\u003c\/td\u003e\n\u003ctd\u003e0.089 M component ≈ 12.1 g\/L\u003c\/td\u003e\n\u003ctd\u003eAcidic phosphate buffer arm\u003c\/td\u003e\n\u003ctd\u003eAccepts protons from organic acid by-products of metabolism; maintains pH above 7.0 during high-density growth; K⁺ supports membrane potential\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eK₂HPO₄\u003c\/td\u003e\n\u003ctd\u003e0.089 M component ≈ 15.5 g\/L\u003c\/td\u003e\n\u003ctd\u003eBasic phosphate buffer arm\u003c\/td\u003e\n\u003ctd\u003eDonates protons when medium becomes alkaline; together with KH₂PO₄ provides ~0.089 M total phosphate — sufficient to buffer cultures to OD₆₀₀ \u0026gt;20 in standard shake flasks\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"sec-title\"\u003eMode of Action — Why TB Outperforms LB for High-Density Culture\u003c\/p\u003e\n\u003cp style=\"font-size: 14px; color: #374151; margin-bottom: 28px; line-height: 1.7;\"\u003eIn LB Broth, growth terminates at OD₆₀₀ 3–5 due to two simultaneous limiting factors: nutrient exhaustion (amino acids and vitamins depleted) and pH drop below 6.5 from accumulated organic acids (acetate, formate). TB addresses both: the enriched nutrient matrix (4× yeast extract, 2× tryptone) delays nutrient limitation to OD₆₀₀ \u0026gt;15, while the 0.089 M potassium phosphate buffer absorbs protons from organic acid metabolism, maintaining pH 7.0–7.4. Glycerol provides additional carbon without acidification. The combined effect extends the exponential growth phase by 6–10 hours in standard shake-flask culture, resulting in 5–10× higher final cell density and proportionally higher yields of all intracellular products.\u003c\/p\u003e\n\u003cp class=\"sec-title\"\u003eQuality Control — Performance Test Organisms\u003c\/p\u003e\n\u003ctable class=\"aus-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eOrganism\u003c\/th\u003e\n\u003cth\u003eATCC \/ Reference\u003c\/th\u003e\n\u003cth\u003eInoculum (CFU)\u003c\/th\u003e\n\u003cth\u003eIncubation\u003c\/th\u003e\n\u003cth\u003eExpected Result\u003c\/th\u003e\n\u003cth\u003eStatus\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eEscherichia coli\u003c\/em\u003e K-12\u003c\/td\u003e\n\u003ctd\u003eATCC 10798\u003c\/td\u003e\n\u003ctd\u003e≤100 CFU\u003c\/td\u003e\n\u003ctd\u003e37 °C, 200 rpm, 18 h\u003c\/td\u003e\n\u003ctd\u003eGood growth; OD₆₀₀ ≥ reference TB lot\u003c\/td\u003e\n\u003ctd class=\"tick\"\u003ePASS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e\n\u003cem\u003eEscherichia coli\u003c\/em\u003e BL21(DE3)\u003c\/td\u003e\n\u003ctd\u003eATCC BAA-1025\u003c\/td\u003e\n\u003ctd\u003e≤100 CFU\u003c\/td\u003e\n\u003ctd\u003e37 °C, 200 rpm, 18 h\u003c\/td\u003e\n\u003ctd\u003eOD₆₀₀ \u0026gt;15 (shake flask, 250 mL \/ 50 mL fill)\u003c\/td\u003e\n\u003ctd class=\"tick\"\u003ePASS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSterility check (uninoculated)\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003ctd\u003e—\u003c\/td\u003e\n\u003ctd\u003e37 °C, 48 h\u003c\/td\u003e\n\u003ctd\u003eNo growth \/ no turbidity increase\u003c\/td\u003e\n\u003ctd class=\"tick\"\u003ePASS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"sec-title\"\u003eLiterature \u0026amp; References\u003c\/p\u003e\n\u003col style=\"font-size: 13px; color: #374151; padding-left: 18px; line-height: 1.9; margin-bottom: 28px;\"\u003e\n\u003cli\u003eTartof, K.D. \u0026amp; Hobbs, C.A. (1987). Improved media for growing plasmid and cosmid clones. \u003cem\u003eBethesda Research Laboratories Focus\u003c\/em\u003e, 9, 12.\u003c\/li\u003e\n\u003cli\u003eSambrook, J. \u0026amp; Russell, D.W. (2001). \u003cem\u003eMolecular Cloning: A Laboratory Manual\u003c\/em\u003e, 3rd edn. Cold Spring Harbor Laboratory Press. Appendix 1.\u003c\/li\u003e\n\u003cli\u003eGreen, M.R. \u0026amp; Sambrook, J. (2012). \u003cem\u003eMolecular Cloning: A Laboratory Manual\u003c\/em\u003e, 4th edn. Cold Spring Harbor Laboratory Press.\u003c\/li\u003e\n\u003cli\u003eStudier, F.W. (2005). Protein production by auto-induction in high-density shaking cultures. \u003cem\u003eProtein Expression and Purification\u003c\/em\u003e, 41(1), 207–234.\u003c\/li\u003e\n\u003cli\u003eRosano, G.L. \u0026amp; Ceccarelli, E.A. (2014). Recombinant protein expression in \u003cem\u003eEscherichia coli\u003c\/em\u003e: advances and challenges. \u003cem\u003eFrontiers in Microbiology\u003c\/em\u003e, 5, 172.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp class=\"sec-title\"\u003eDownload TDS Document\u003c\/p\u003e\n\u003cdiv class=\"doc-grid\"\u003e\u003ca href=\"#\" class=\"doc-btn\"\u003e \u003csvg viewbox=\"0 0 16 16\" fill=\"none\" stroke=\"currentColor\" stroke-width=\"1.5\"\u003e\u003cpath d=\"M4 1h6l4 4v10H4V1z\"\u003e\u003c\/path\u003e\u003cpath d=\"M10 1v4h4\"\u003e\u003c\/path\u003e\u003cpath d=\"M8 7v5M5.5 9.5L8 12l2.5-2.5\"\u003e\u003c\/path\u003e\u003c\/svg\u003e TDS — AS-1429 Terrific Broth (PDF) \u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv class=\"disclaimer\"\u003e\n\u003cstrong\u003eAuSaMicS Pty Ltd Disclaimer:\u003c\/strong\u003e Technical data is based on current literature and internal quality testing. Values may vary by lot. For laboratory use only.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- \/tb-tds --\u003e \u003c!-- ================================================================ TAB 3: SDS --\u003e\n\u003cdiv id=\"tb-sds\" class=\"aus-panel\"\u003e\n\u003cp class=\"sec-title\"\u003eSafety Data Sheet — AS-1429 Terrific Broth\u003c\/p\u003e\n\u003cp style=\"font-size: 14px; color: #374151; margin-bottom: 20px; line-height: 1.7;\"\u003eFull 16-section Safety Data Sheet compliant with GHS and Australian Work Health and Safety (WHS) Regulations. Download the complete SDS before handling this product.\u003c\/p\u003e\n\u003cdiv class=\"quick-specs\"\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eGHS Standard\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003eGHS Rev. 9\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eWHS Compliance\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003eAustralian WHS Reg. 2017\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eSections\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003e16 mandatory\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eSignal Word\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003eNot classified\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp class=\"sec-title\"\u003eSDS Section Summary\u003c\/p\u003e\n\u003ctable class=\"aus-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eSection\u003c\/th\u003e\n\u003cth\u003eHeading\u003c\/th\u003e\n\u003cth\u003eKey Information\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003e1\u003c\/td\u003e\n\u003ctd\u003eIdentification\u003c\/td\u003e\n\u003ctd\u003eTerrific Broth, AS-1429, AuSaMicS Pty Ltd, support@ausamics.com, +61 412 520 598\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2\u003c\/td\u003e\n\u003ctd\u003eHazard identification\u003c\/td\u003e\n\u003ctd\u003eNot classified as hazardous under GHS. No signal word. Dust may cause mild respiratory irritation.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3\u003c\/td\u003e\n\u003ctd\u003eComposition \/ ingredients\u003c\/td\u003e\n\u003ctd\u003eTryptone, Yeast Extract, Glycerol, KH₂PO₄, K₂HPO₄ — all non-hazardous biological\/food-grade materials\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e4\u003c\/td\u003e\n\u003ctd\u003eFirst aid\u003c\/td\u003e\n\u003ctd\u003eInhalation: fresh air. Skin\/eyes: wash with water 15 min. Ingestion: rinse mouth; seek advice if unwell.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5\u003c\/td\u003e\n\u003ctd\u003eFirefighting\u003c\/td\u003e\n\u003ctd\u003eNot flammable. CO₂ or dry powder extinguisher. SCBA in enclosed fire scenarios.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e6\u003c\/td\u003e\n\u003ctd\u003eAccidental release\u003c\/td\u003e\n\u003ctd\u003eSweep up powder; avoid dust generation. Prepared broth: absorb with inert material; dispose per local regulations.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e7\u003c\/td\u003e\n\u003ctd\u003eHandling \u0026amp; storage\u003c\/td\u003e\n\u003ctd\u003eStore powder at 2–25 °C, sealed, dry. Prepared medium at 2–8 °C, use within 4 weeks.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e8\u003c\/td\u003e\n\u003ctd\u003eExposure controls \/ PPE\u003c\/td\u003e\n\u003ctd\u003eLab coat, nitrile gloves, safety glasses. No respiratory protection required for normal use.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e9\u003c\/td\u003e\n\u003ctd\u003ePhysical \u0026amp; chemical properties\u003c\/td\u003e\n\u003ctd\u003eGolden-yellow powder (dry); amber liquid (prepared). Not flammable. pH 7.2 prepared.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10\u003c\/td\u003e\n\u003ctd\u003eStability \u0026amp; reactivity\u003c\/td\u003e\n\u003ctd\u003eStable. Incompatible with strong oxidisers. No hazardous decomposition under normal conditions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e11\u003c\/td\u003e\n\u003ctd\u003eToxicological information\u003c\/td\u003e\n\u003ctd\u003eNot acutely toxic. All components have low oral, dermal, and inhalation toxicity profiles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e12\u003c\/td\u003e\n\u003ctd\u003eEcological information\u003c\/td\u003e\n\u003ctd\u003eBiodegradable. Not environmentally hazardous. Avoid large releases to waterways (high BOD).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e13\u003c\/td\u003e\n\u003ctd\u003eDisposal\u003c\/td\u003e\n\u003ctd\u003eAutoclave inoculated broth before disposal. Uninoculated: dilute and drain. Dry powder: general lab waste.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e14\u003c\/td\u003e\n\u003ctd\u003eTransport\u003c\/td\u003e\n\u003ctd\u003eNot classified as dangerous goods (UN\/ADG). No special transport requirements.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e15\u003c\/td\u003e\n\u003ctd\u003eRegulatory\u003c\/td\u003e\n\u003ctd\u003eNot subject to AICIS notification. Complies with Australian WHS model regulations.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e16\u003c\/td\u003e\n\u003ctd\u003eOther information\u003c\/td\u003e\n\u003ctd\u003eIssued by AuSaMicS Pty Ltd. For laboratory use only.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"sec-title\"\u003eDownload Full SDS (16-Section, GHS-Compliant)\u003c\/p\u003e\n\u003cdiv class=\"doc-grid\"\u003e\u003ca href=\"#\" class=\"doc-btn\"\u003e \u003csvg viewbox=\"0 0 16 16\" fill=\"none\" stroke=\"currentColor\" stroke-width=\"1.5\"\u003e\u003cpath d=\"M4 1h6l4 4v10H4V1z\"\u003e\u003c\/path\u003e\u003cpath d=\"M10 1v4h4\"\u003e\u003c\/path\u003e\u003cpath d=\"M8 7v5M5.5 9.5L8 12l2.5-2.5\"\u003e\u003c\/path\u003e\u003c\/svg\u003e SDS — AS-1429 Terrific Broth (PDF) \u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv class=\"disclaimer\"\u003e\n\u003cstrong\u003eAuSaMicS Pty Ltd Safety Disclaimer:\u003c\/strong\u003e Always consult the full SDS before handling. Use appropriate PPE. Emergency contact: +61 412 520 598.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- \/tb-sds --\u003e \u003c!-- ================================================================ TAB 4: COA --\u003e\n\u003cdiv id=\"tb-coa\" class=\"aus-panel\"\u003e\n\u003cp class=\"sec-title\"\u003eCertificate of Analysis — AS-1429 Terrific Broth\u003c\/p\u003e\n\u003cp style=\"font-size: 14px; color: #374151; margin-bottom: 20px; line-height: 1.7;\"\u003eA batch-specific Certificate of Analysis is issued for every production lot of AS-1429. The COA confirms that the supplied lot meets all specifications below. Retain for laboratory audit and accreditation purposes.\u003c\/p\u003e\n\u003cdiv class=\"quick-specs\"\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eIssued by\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003eAuSaMicS Pty Ltd\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eTest frequency\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003eEvery production lot\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eTraceability\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003eLOT \u0026amp; EXP on label\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"qs-card\"\u003e\n\u003cdiv class=\"qs-label\"\u003eDispatch\u003c\/div\u003e\n\u003cdiv class=\"qs-val\"\u003eIncluded with order\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp class=\"sec-title\"\u003eSpecification Table\u003c\/p\u003e\n\u003ctable class=\"aus-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eTest Parameter\u003c\/th\u003e\n\u003cth\u003eMethod\u003c\/th\u003e\n\u003cth\u003eSpecification\u003c\/th\u003e\n\u003cth\u003eTypical Result\u003c\/th\u003e\n\u003cth\u003eStatus\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (powder)\u003c\/td\u003e\n\u003ctd\u003eVisual\u003c\/td\u003e\n\u003ctd\u003eCream to golden-yellow, homogeneous, free-flowing\u003c\/td\u003e\n\u003ctd\u003eGolden-yellow, homogeneous\u003c\/td\u003e\n\u003ctd class=\"tick\"\u003ePASS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance (prepared broth)\u003c\/td\u003e\n\u003ctd\u003eVisual\u003c\/td\u003e\n\u003ctd\u003eGolden-yellow to amber, slightly turbid\u003c\/td\u003e\n\u003ctd\u003eAmber, slightly turbid\u003c\/td\u003e\n\u003ctd class=\"tick\"\u003ePASS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH (prepared, 25 °C)\u003c\/td\u003e\n\u003ctd\u003ePotentiometry\u003c\/td\u003e\n\u003ctd\u003e7.2 ± 0.2\u003c\/td\u003e\n\u003ctd\u003e7.2\u003c\/td\u003e\n\u003ctd class=\"tick\"\u003ePASS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMoisture content\u003c\/td\u003e\n\u003ctd\u003eLoss on drying\u003c\/td\u003e\n\u003ctd\u003e≤6.0%\u003c\/td\u003e\n\u003ctd\u003e4.1%\u003c\/td\u003e\n\u003ctd class=\"tick\"\u003ePASS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGrowth promotion — \u003cem\u003eE. coli\u003c\/em\u003e K-12 ATCC 10798\u003c\/td\u003e\n\u003ctd\u003eOD₆₀₀ \/ incubation\u003c\/td\u003e\n\u003ctd\u003eOD₆₀₀ ≥ reference TB lot at 18 h\u003c\/td\u003e\n\u003ctd\u003eOD₆₀₀ \u0026gt;15 at 18 h, 37 °C, 200 rpm\u003c\/td\u003e\n\u003ctd class=\"tick\"\u003ePASS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH stability post-growth (high-density)\u003c\/td\u003e\n\u003ctd\u003ePotentiometry post-culture\u003c\/td\u003e\n\u003ctd\u003epH ≥ 6.8 at OD₆₀₀ = 15\u003c\/td\u003e\n\u003ctd\u003epH 7.0 at OD₆₀₀ 15+\u003c\/td\u003e\n\u003ctd class=\"tick\"\u003ePASS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSterility (prepared medium)\u003c\/td\u003e\n\u003ctd\u003eIncubation 37 °C \/ 48 h\u003c\/td\u003e\n\u003ctd\u003eNo growth\u003c\/td\u003e\n\u003ctd\u003eNo growth\u003c\/td\u003e\n\u003ctd class=\"tick\"\u003ePASS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp class=\"sec-title\"\u003eDownload Sample COA\u003c\/p\u003e\n\u003cdiv class=\"doc-grid\"\u003e\u003ca href=\"#\" class=\"doc-btn\"\u003e \u003csvg viewbox=\"0 0 16 16\" fill=\"none\" stroke=\"currentColor\" stroke-width=\"1.5\"\u003e\u003cpath d=\"M4 1h6l4 4v10H4V1z\"\u003e\u003c\/path\u003e\u003cpath d=\"M10 1v4h4\"\u003e\u003c\/path\u003e\u003cpath d=\"M8 7v5M5.5 9.5L8 12l2.5-2.5\"\u003e\u003c\/path\u003e\u003c\/svg\u003e COA — AS-1429 Terrific Broth (Sample PDF) \u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv class=\"disclaimer\"\u003e\n\u003cstrong\u003eAuSaMicS Pty Ltd COA Disclaimer:\u003c\/strong\u003e This Certificate of Analysis is lot-specific. The sample COA is representative only. The actual COA for the supplied lot is included with every order. For lot-specific documentation: support@ausamics.com.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- \/tb-coa --\u003e \u003c!-- FOOTER --\u003e\n\u003cdiv class=\"aus-footer\"\u003e\n\u003cstrong\u003eAuSaMicS Pty Ltd\u003c\/strong\u003e | ABN 56 676 640 467\u003cbr\u003e31 Longview CT, Thomastown VIC 3074, Australia\u003cbr\u003e\u003ca href=\"https:\/\/www.ausamics.com.au\"\u003ewww.ausamics.com.au\u003c\/a\u003e | \u003ca href=\"mailto:support@ausamics.com\"\u003esupport@ausamics.com\u003c\/a\u003e | +61 412 520 598\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- \/aus-tb --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- TAB SCRIPT --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cstyle\u003e\n  .aus-tb *{box-sizing:border-box;margin:0;padding:0;}\n  .aus-tb{font-family:'Segoe UI',system-ui,-apple-system,sans-serif;color:#1a1a1a;line-height:1.65;max-width:900px;}\n\n  .aus-tabs{display:flex;gap:0;border-bottom:2px solid #e5e7eb;margin-bottom:28px;flex-wrap:wrap;}\n  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