{"product_id":"tpgy-broth-base-w-o-trypsin","title":"TPGY Broth Base w\/o Trypsin | Tryptone Peptone Glucose Yeast Extract | AS‑1366","description":"\u003c!-- LLM\/AI-Agent Optimization Meta Snippet --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- product: Tryptone Peptone Glucose Yeast Extract (TPGY) Broth Base, w\/o Trypsin; catalog: AS-1366; manufacturer: AuSaMicS Pty Ltd; standard: FDA BAM Ch17, AOAC 977.26, USDA FSIS MLG 10; equivalents: Oxoid CM0877, BD Difco 299088, Merck 1.05359; target: Clostridium botulinum, anaerobic spore-formers --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- Intent-based long-tail search queries: TPGY Broth Clostridium botulinum food safety testing Australia; Tryptone Peptone Glucose Yeast Extract Broth Base AS-1366 AuSaMicS; FDA BAM Chapter 17 botulism enrichment medium Australia; TPGY without trypsin anaerobic enrichment broth; buy TPGY broth Australia laboratory; Oxoid CM0877 equivalent Australia TPGY; AOAC 977.26 botulism enrichment broth; anaerobic enrichment broth food microbiology Australia; Clostridium botulinum culture medium; TPGY broth base dehydrated powder supplier --\u003e\n\u003cdiv class=\"aus-tpgy-product\" style=\"font-family: 'Segoe UI',system-ui,-apple-system,sans-serif; color: #1d2935; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 20px;\"\u003e\n\u003cdiv class=\"aus-tpgy-hero-section\" style=\"background: linear-gradient(135deg,#f0f4f8 0%,#dce8f0 100%); border-radius: 18px; padding: 40px; margin-bottom: 30px; box-shadow: 0 4px 15px rgba(0,0,0,0.08); border: 1px solid #4a7fa5;\"\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr auto; gap: 30px; align-items: start;\" class=\"aus-tpgy-hero-grid\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"display: inline-block; font-size: 12px; letter-spacing: .08em; text-transform: uppercase; color: #1a3a52; font-weight: bold; margin-bottom: 12px; background: #d0e4f0; padding: 6px 12px; border-radius: 20px;\"\u003eAuSaMicS Life Science • Anaerobic Enrichment Culture Media\u003c\/div\u003e\n\u003ch1 style=\"color: #0d2233; margin: 0 0 8px 0; font-size: 32px; font-weight: bold;\"\u003eTryptone Peptone Glucose Yeast Extract (TPGY) Broth Base\u003c\/h1\u003e\n\u003ch2 style=\"color: #1a3a52; margin: 0 0 15px 0; font-weight: 400; font-size: 20px; opacity: 0.9;\"\u003ew\/o Trypsin — \u003cem\u003eClostridium botulinum\u003c\/em\u003e \u0026amp; Anaerobic Spore-Former Enrichment\u003c\/h2\u003e\n\u003cp style=\"font-size: 17px; margin: 0 0 20px 0; color: #2d3748; max-width: 620px; font-weight: 500;\"\u003eThe gold-standard enrichment broth for \u003cstrong\u003erecovery and cultivation of \u003cem\u003eClostridium botulinum\u003c\/em\u003e and anaerobic spore-forming bacteria\u003c\/strong\u003e from food, environmental, and clinical specimens. Formulated without trypsin — trypsin is added separately as required for neurotoxin activation in botulism testing workflows per \u003cstrong\u003eFDA BAM Chapter 17\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cdiv style=\"display: flex; gap: 10px; flex-wrap: wrap; margin-bottom: 20px;\"\u003e\n\u003cspan style=\"background: #0d2233; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eCat #: AS-1366\u003c\/span\u003e \u003cspan style=\"background: #fff; border: 2px solid #1a3a52; color: #1a3a52; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eFDA BAM Ch. 17 Compliant\u003c\/span\u003e \u003cspan style=\"background: #28a745; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003e✓ Australian Stock\u003c\/span\u003e \u003cspan style=\"background: #4a7fa5; color: #fff; padding: 8px 16px; border-radius: 25px; font-size: 13px; font-weight: 600; white-space: nowrap;\"\u003eWithout Trypsin\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; gap: 20px; flex-wrap: wrap; font-size: 14px; color: #1a3a52;\"\u003e\n\u003cspan\u003e🚀 \u003cstrong\u003eSame-Day Dispatch:\u003c\/strong\u003e Australian Stock\u003c\/span\u003e \u003cspan\u003e🧪 \u003cstrong\u003eBotulism Testing:\u003c\/strong\u003e FDA BAM \/ AOAC 977.26\u003c\/span\u003e \u003cspan\u003e🔬 \u003cstrong\u003eIncubation:\u003c\/strong\u003e 35–37°C \/ 5–7 days anaerobic\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; box-shadow: 0 6px 20px rgba(0,0,0,0.10); min-width: 280px; border: 1px solid #4a7fa5;\" class=\"aus-tpgy-guarantee\"\u003e\n\u003ch4 style=\"color: #0d2233; margin: 0 0 15px 0; text-align: center; font-size: 16px;\"\u003e🏋 Botulism Enrichment Specialist\u003c\/h4\u003e\n\u003cdiv style=\"font-size: 14px; color: #4a5568; text-align: center; line-height: 1.5;\"\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e🛒 \u003cstrong\u003eNo Trypsin Included\u003c\/strong\u003e — Add separately as required\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e🧬 \u003cstrong\u003eHighly Nutritious Formula\u003c\/strong\u003e — Supports fastidious anaerobes\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e📋 \u003cstrong\u003eComplete Package\u003c\/strong\u003e — COA + SDS included\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e⚡ \u003cstrong\u003eFast Supply\u003c\/strong\u003e — Ships within 24h\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 12px;\"\u003e📞 \u003cstrong\u003eExpert Support\u003c\/strong\u003e — Protocol optimization\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-top: 20px; padding: 15px; background: #f0f4f8; border-radius: 12px; text-align: center; border: 1px solid #b8d0e0;\"\u003e\n\u003cdiv style=\"color: #1a3a52; font-weight: 600; font-size: 14px;\"\u003eStatic \u0026amp; Agitated Anaerobic Culture\u003c\/div\u003e\n\u003cdiv style=\"color: #4a5568; font-size: 13px; margin-top: 5px;\"\u003eValidated for both static enrichment and gentle agitation anaerobic protocols\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1.2fr 0.8fr; gap: 40px; margin-bottom: 40px;\" class=\"aus-tpgy-main-grid\"\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #0d2233; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #1a3a52;\"\u003e🔬 Technical Overview \u0026amp; Biochemistry\u003c\/h3\u003e\n\u003cdiv style=\"background: #f0f4f8; padding: 20px; border-radius: 16px; border-left: 5px solid #1a3a52;\"\u003e\n\u003cp style=\"margin: 0 0 15px 0;\"\u003eTPGY Broth Base is a \u003cstrong\u003erich, non-selective enrichment medium\u003c\/strong\u003e formulated for isolation and cultivation of \u003cem\u003eClostridium botulinum\u003c\/em\u003e and other obligate anaerobic spore-forming bacteria from food, environmental, and clinical specimens. Supplied \u003cstrong\u003ewithout trypsin\u003c\/strong\u003e — add trypsin separately for neurotoxin activation per FDA BAM Chapter 17.\u003c\/p\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; font-size: 14px; color: #2d3748; border: 1px solid #b8d0e0; margin-bottom: 15px;\"\u003e\n\u003cstrong\u003eNutritional Components:\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eTryptone\u003c\/strong\u003e — Enzymatic casein digest; rich peptides and amino acids for fastidious anaerobes\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePeptone\u003c\/strong\u003e — Meat-derived protein digest; supplementary nitrogen and micronutrients\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eGlucose (Dextrose)\u003c\/strong\u003e — Fermentable carbohydrate; drives anaerobiosis via CO₂ production\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eYeast Extract\u003c\/strong\u003e — B-vitamins, nucleotide precursors, trace minerals for obligate anaerobes\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSodium Thioglycollate\u003c\/strong\u003e — Reduces Eh; creates the reduced microenvironment required for spore germination\u003c\/div\u003e\n\u003cdiv style=\"background: #dce8f0; padding: 12px; border-radius: 8px; border: 1px solid #b8d0e0;\"\u003e\n\u003cstrong style=\"color: #1a3a52;\"\u003eWhy Without Trypsin?\u003c\/strong\u003e Trypsin activates the progenitor toxin complex into its fully toxic form prior to mouse bioassay or ELISA confirmation. Supplying the base without trypsin gives laboratories full flexibility to add it at the precise workflow stage.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #0d2233; font-size: 22px; margin: 0 0 15px 0; padding-bottom: 10px; border-bottom: 3px solid #1a3a52;\"\u003e⚙️ Mode of Action\u003c\/h3\u003e\n\u003cdiv style=\"background: #f0f4f8; padding: 20px; border-radius: 16px; border-left: 5px solid #4a7fa5;\"\u003e\n\u003cp style=\"margin: 0; font-size: 15px; color: #2d3748;\"\u003eTPGY Broth provides the combined nutritional, reductive, and osmotic conditions for \u003cem\u003eC. botulinum\u003c\/em\u003e spore germination and vegetative growth under anaerobic incubation. Glucose fermentation drives down dissolved O₂ and redox potential, complementing sodium thioglycollate to create a strictly anaerobic microenvironment. Yeast extract supplies growth cofactors that overcome biosynthetic limitations of obligate anaerobes, significantly enhancing recovery from heavily processed food matrices where spore populations may be low-density or sub-lethally stressed.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #0d2233; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #1a3a52;\"\u003e📊 Anaerobic Enrichment Media Comparison\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #b8d0e0; border-radius: 12px; overflow: hidden; box-shadow: 0 2px 8px rgba(0,0,0,0.05); min-width: 820px;\"\u003e\n\u003cthead style=\"background: #dce8f0;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #b8d0e0; text-align: left; color: #0d2233;\"\u003eMedium\u003c\/th\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #b8d0e0; text-align: left; color: #0d2233;\"\u003eType\u003c\/th\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #b8d0e0; text-align: left; color: #0d2233;\"\u003ePrimary Target\u003c\/th\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #b8d0e0; text-align: left; color: #0d2233;\"\u003eKey Feature\u003c\/th\u003e\n\u003cth style=\"padding: 15px 12px; border-bottom: 2px solid #b8d0e0; text-align: left; color: #0d2233;\"\u003ePrimary Use\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #e8f0f7; border-bottom: 1px solid #b8d0e0;\"\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; color: #1a3a52;\"\u003eTPGY Broth Base \u003cspan style=\"background: #1a3a52; color: #fff; font-size: 10px; padding: 2px 6px; border-radius: 10px; margin-left: 5px;\"\u003eTHIS PRODUCT\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eNon-selective Enrichment Broth\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; font-weight: 600;\"\u003e\n\u003cem\u003eC. botulinum\u003c\/em\u003e, anaerobic spore-formers\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #0d2233; font-weight: 600;\"\u003eNo trypsin; add separately for neurotoxin activation\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eBotulism testing — FDA BAM Ch.17, AOAC 977.26\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003e\u003ca href=\"\/products\/thioglycollate-medium-fluid\" style=\"color: #1a3a52; text-decoration: none; font-weight: 600;\"\u003eThioglycollate Broth (Fluid)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eNon-selective Enrichment Broth\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eAerobes, anaerobes, microaerophiles\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #4a5568;\"\u003eBroad-spectrum; sterility testing (USP, Ph.Eur.)\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eSterility testing, blood culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003e\u003ca href=\"\/products\/reinforced-clostridial-medium\" style=\"color: #1a3a52; text-decoration: none; font-weight: 600;\"\u003eReinforced Clostridial Medium (RCM)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eNon-selective Enrichment\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eClostridia (all species)\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #4a5568;\"\u003eGeneral Clostridial recovery; solid and liquid formats\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eNon-selective anaerobe recovery, spore counts\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003e\u003ca href=\"\/products\/differential-reinforced-clostridial-medium\" style=\"color: #1a3a52; text-decoration: none; font-weight: 600;\"\u003eDifferential Reinforced Clostridial Medium (DRCM)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eSelective + Differential Broth\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eSulfite-reducing Clostridia (SRC)\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #4a5568;\"\u003eBlack turbid broth (FeS) — water quality SRC\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eWater quality — APHA 9060C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px;\"\u003e\u003ca href=\"\/products\/cooked-meat-medium\" style=\"color: #1a3a52; text-decoration: none; font-weight: 600;\"\u003eCooked Meat Medium (CMM)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eEnrichment Broth\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eAnaerobes, \u003cem\u003eClostridium\u003c\/em\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #4a5568;\"\u003eMeat particles scavenge O₂; long-term maintenance\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eAnaerobe preservation, sub-culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px;\"\u003e\u003ca href=\"\/products\/sulfite-iron-agar-base\" style=\"color: #1a3a52; text-decoration: none; font-weight: 600;\"\u003eSulfite Iron Agar Base (SIA)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eSelective + Differential Agar\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003e\n\u003cem\u003eC. perfringens\u003c\/em\u003e, SRC\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; color: #4a5568;\"\u003eJet-black colonies (FeS precipitation)\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eWater \/ food — ISO 14189, AS\/NZS 4276.19\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #0d2233; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #1a3a52;\"\u003e🧬 Applications\u003c\/h3\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit,minmax(280px,1fr)); gap: 20px; margin-bottom: 25px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #b8d0e0; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n\u003ch4 style=\"color: #1a3a52; margin: 0 0 10px 0; font-size: 16px;\"\u003e🧪 Food Safety — Botulism Testing\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003ePrimary enrichment broth for \u003cem\u003eC. botulinum\u003c\/em\u003e detection in low-acid canned foods, meat products, fish, honey, and fermented foods per FDA BAM Chapter 17 and AOAC 977.26. Trypsin added post-enrichment for neurotoxin activation prior to mouse bioassay or ELISA confirmation.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #b8d0e0; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n\u003ch4 style=\"color: #1a3a52; margin: 0 0 10px 0; font-size: 16px;\"\u003e🛒 Wound \u0026amp; Infant Botulism\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eAnaerobic enrichment of wound swabs, tissue samples, and stool for \u003cem\u003eC. botulinum\u003c\/em\u003e recovery in wound botulism and infant botulism case investigation. Used in reference public health and clinical microbiology laboratories.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #b8d0e0; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n\u003ch4 style=\"color: #1a3a52; margin: 0 0 10px 0; font-size: 16px;\"\u003e🌍 Environmental \u0026amp; Soil Monitoring\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eRecovery of \u003cem\u003eC. botulinum\u003c\/em\u003e spores from soil, sediment, compost, and water samples. Used in ecological surveys, agricultural land risk assessments, and avian botulism investigations.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #b8d0e0; box-shadow: 0 2px 8px rgba(0,0,0,0.05);\"\u003e\n\u003ch4 style=\"color: #1a3a52; margin: 0 0 10px 0; font-size: 16px;\"\u003e🔬 Anaerobic Research\u003c\/h4\u003e\n\u003cp style=\"margin: 0; font-size: 14px; color: #4a5568;\"\u003eGeneral enrichment and maintenance of obligate anaerobes and \u003cem\u003eClostridium\u003c\/em\u003e spp. in research settings. Supports anaerobe physiology, sporulation studies, toxin gene expression, and industrial anaerobic fermentation.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #dce8f0; padding: 20px; border-radius: 16px; border-left: 5px solid #1a3a52;\"\u003e\n\u003ch4 style=\"color: #0d2233; margin: 0 0 15px 0; font-size: 16px;\"\u003eAdditional Laboratory Applications:\u003c\/h4\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit,minmax(250px,1fr)); gap: 15px;\"\u003e\n\u003cul style=\"margin: 0; padding-left: 20px; color: #4a5568; font-size: 14px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eInfant Botulism:\u003c\/strong\u003e Stool enrichment for \u003cem\u003eC. botulinum\u003c\/em\u003e spore recovery\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNATA Accredited Labs:\u003c\/strong\u003e Botulism reference testing panels\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOutbreak Investigation:\u003c\/strong\u003e Foodborne botulism source tracing\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"margin: 0; padding-left: 20px; color: #4a5568; font-size: 14px;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003ePharmaceutical QC:\u003c\/strong\u003e Supplementary anaerobe sterility testing\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAquaculture:\u003c\/strong\u003e Sediment and fish tissue \u003cem\u003eC. botulinum\u003c\/em\u003e monitoring\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIndustrial Fermentation:\u003c\/strong\u003e Anaerobic process organism cultivation\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 35px;\"\u003e\n\u003ch3 style=\"color: #0d2233; font-size: 22px; margin: 0 0 20px 0; padding-bottom: 10px; border-bottom: 3px solid #1a3a52;\"\u003e📋 Recommended Procedure (FDA BAM Chapter 17 \/ AOAC 977.26)\u003c\/h3\u003e\n\u003cdiv style=\"background: #f0f4f8; padding: 20px; border-radius: 16px; border-left: 5px solid #1a3a52;\"\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 20px; margin-bottom: 20px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 12px; border: 2px solid #1a3a52;\"\u003e\n\u003ch5 style=\"color: #0d2233; margin: 0 0 12px 0; font-size: 15px; text-align: center;\"\u003e🧪 Method A — Food Sample Enrichment\u003c\/h5\u003e\n\u003col style=\"margin: 0; padding-left: 18px; font-size: 13px; color: #4a5568; line-height: 1.8;\"\u003e\n\u003cli\u003eHomogenise 10 g food sample in 90 mL TPGY Broth (1:10 dilution)\u003c\/li\u003e\n\u003cli\u003eTransfer aliquots into TPGY Broth tubes\u003c\/li\u003e\n\u003cli\u003eHeat-shock duplicate aliquots at 80°C \/ 15 min (spore selection)\u003c\/li\u003e\n\u003cli\u003eIncubate heated and unheated at 35°C under strict anaerobic conditions for 5–7 days\u003c\/li\u003e\n\u003cli\u003eFor toxin testing: add trypsin (0.1% final) to turbid cultures; 37°C \/ 30 min before ELISA\/bioassay\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 12px; border: 2px dashed #4a7fa5;\"\u003e\n\u003ch5 style=\"color: #0d2233; margin: 0 0 12px 0; font-size: 15px; text-align: center;\"\u003e🔬 Method B — Environmental Enrichment\u003c\/h5\u003e\n\u003col style=\"margin: 0; padding-left: 18px; font-size: 13px; color: #4a5568; line-height: 1.8;\"\u003e\n\u003cli\u003eSuspend 1–5 g soil\/sediment in 20 mL TPGY Broth\u003c\/li\u003e\n\u003cli\u003eMix; allow large particles to settle (10 min)\u003c\/li\u003e\n\u003cli\u003eTransfer supernatant to fresh TPGY Broth tubes\u003c\/li\u003e\n\u003cli\u003eHeat-shock 80°C \/ 15 min for spore selection\u003c\/li\u003e\n\u003cli\u003eIncubate 35–37°C anaerobic \/ 7–14 days; subculture to isolation agar\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; border: 2px solid #4a7fa5; border-radius: 12px; padding: 15px; margin-bottom: 12px;\"\u003e\n\u003ch5 style=\"color: #0d2233; margin: 0 0 10px 0; font-size: 14px;\"\u003e🪲 Trypsin Activation Protocol (for Toxin Testing)\u003c\/h5\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit,minmax(200px,1fr)); gap: 10px; font-size: 13px; color: #4a5568;\"\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #1a3a52;\"\u003eTrypsin Source:\u003c\/strong\u003e Use trypsin from porcine pancreas (activity: 1:250); add to 0.1–0.5% (w\/v) final concentration in enrichment culture\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #1a3a52;\"\u003eActivation Conditions:\u003c\/strong\u003e 37°C for 30–60 min; gentle mixing. Do NOT overheat — excessive heat inactivates the toxin\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #1a3a52;\"\u003eNeutralisation:\u003c\/strong\u003e After activation, add soybean trypsin inhibitor (SBTI) if required before downstream testing to stop trypsin activity\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #1a3a52;\"\u003eSafety Note:\u003c\/strong\u003e Handle trypsin-activated cultures as Biosafety Level 2 (BSL-2) material; follow institutional botulinum toxin handling procedures\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff3cd; padding: 12px; border-radius: 8px; border: 1px solid #ffc107;\"\u003e\n\u003cstrong style=\"color: #856404;\"\u003e⚠️ Critical:\u003c\/strong\u003e \u003cspan style=\"font-size: 14px; color: #856404;\"\u003eStrict anaerobic conditions are required throughout incubation. Prepare and dispense TPGY Broth freshly before use; pre-reduce medium in anaerobic chamber if available. For spore-selective testing, heat-shock at 80°C \/ 15 min is mandatory to destroy vegetative cells.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: linear-gradient(135deg,#fff3e0 0%,#ffe0b3 100%); padding: 20px; border-radius: 16px; border-left: 5px solid #ff9800; margin-bottom: 35px;\"\u003e\n\u003ch4 style=\"color: #e65100; margin: 0 0 15px 0; font-size: 16px;\"\u003e💡 Protocol Optimization Guidelines\u003c\/h4\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit,minmax(220px,1fr)); gap: 15px;\"\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #e65100;\"\u003ePreparation:\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eDissolve 33.0 g\/L in purified water. Heat gently to dissolve. Autoclave 121°C \/ 15 min. Cool to 48–50°C before dispensing to tubes.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #e65100;\"\u003epH (25°C):\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003e7.2 ± 0.2 after sterilisation\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #e65100;\"\u003ePre-reduction:\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003eFor optimal spore recovery, pre-reduce broth in anaerobic jar or chamber for 24 h before inoculation, especially with dry or aged samples.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cstrong style=\"color: #e65100;\"\u003eStorage (prepared broth):\u003c\/strong\u003e\u003cbr\u003e\u003cspan style=\"font-size: 14px; color: #5d4037;\"\u003e2–8°C, use within 2 weeks. Dehydrated powder: below 25°C, dry and dark. Discard if medium shows turbidity or discolouration before use.\u003c\/span\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- RIGHT COLUMN --\u003e\n\u003cdiv\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #4a7fa5; box-shadow: 0 4px 12px rgba(0,0,0,0.08); margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #0d2233; margin: 0 0 20px 0; font-size: 18px; text-align: center;\"\u003e📋 Technical Specifications\u003c\/h3\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 14px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #b8d0e0;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #0d2233; width: 45%; background: #f0f4f8;\"\u003eCatalogue Number\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eAS-1366\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #b8d0e0;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #0d2233; background: #f0f4f8;\"\u003eCommon Name\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eTPGY Broth \/ TPGY Medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #b8d0e0;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #0d2233; background: #f0f4f8;\"\u003eFormat\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eDehydrated powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #b8d0e0;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #0d2233; background: #f0f4f8;\"\u003eTrypsin\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #c0392b; font-weight: 600;\"\u003eNot included — add separately\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #b8d0e0;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #0d2233; background: #f0f4f8;\"\u003eReconstitution\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e~33.0 g\/L in purified water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #b8d0e0;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #0d2233; background: #f0f4f8;\"\u003epH (25°C)\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e7.2 ± 0.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #b8d0e0;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #0d2233; background: #f0f4f8;\"\u003eIncubation Temp\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #1a3a52;\"\u003e35–37°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #b8d0e0;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #0d2233; background: #f0f4f8;\"\u003eIncubation Time\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e5–7 days (food); 7–14 days (environmental)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #b8d0e0;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #0d2233; background: #f0f4f8;\"\u003eAtmosphere\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #1a3a52; font-weight: 600;\"\u003eStrictly anaerobic\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #b8d0e0;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #0d2233; background: #f0f4f8;\"\u003eGrowth Indicator\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #1a3a52; font-weight: 600;\"\u003eTurbidity \/ gas production\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #b8d0e0;\"\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #0d2233; background: #f0f4f8;\"\u003eSterilisation\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003eAutoclave 121°C \/ 15 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px 8px; font-weight: 600; color: #0d2233; background: #f0f4f8;\"\u003eHS Code\u003c\/td\u003e\n\u003ctd style=\"padding: 12px 8px; color: #4a5568;\"\u003e3821.00.00\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #dce8f0; padding: 20px; border-radius: 16px; border: 1px solid #b8d0e0; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #0d2233; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e✓ Quality Control Performance\u003c\/h4\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background: #c8dcea;\"\u003e\n\u003cth style=\"padding: 8px; text-align: left; color: #0d2233; border-bottom: 1px solid #b8d0e0;\"\u003eTest Organism\u003c\/th\u003e\n\u003cth style=\"padding: 8px; text-align: left; color: #0d2233; border-bottom: 1px solid #b8d0e0;\"\u003eATCC\u003c\/th\u003e\n\u003cth style=\"padding: 8px; text-align: left; color: #0d2233; border-bottom: 1px solid #b8d0e0;\"\u003eExpected Result\u003c\/th\u003e\n\u003cth style=\"padding: 8px; text-align: left; color: #0d2233; border-bottom: 1px solid #b8d0e0;\"\u003eCFU Spec\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #c8dcea;\"\u003e\n\u003ctd style=\"padding: 8px; color: #2d3748;\"\u003e\u003cem\u003eClostridium sporogenes\u003c\/em\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e19404\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #0d2233; font-weight: 600;\"\u003eGood growth, turbidity\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e≤100 CFU\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #c8dcea;\"\u003e\n\u003ctd style=\"padding: 8px; color: #2d3748;\"\u003e\u003cem\u003eClostridium perfringens\u003c\/em\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e13124\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #0d2233; font-weight: 600;\"\u003eGood growth, turbidity\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e≤100 CFU\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #c8dcea;\"\u003e\n\u003ctd style=\"padding: 8px; color: #2d3748;\"\u003e\u003cem\u003eBacteroides fragilis\u003c\/em\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e25285\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #0d2233; font-weight: 600;\"\u003eGood growth\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e≤100 CFU\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; color: #2d3748;\"\u003e\u003cem\u003eEscherichia coli\u003c\/em\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e25922\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #dc3545; font-weight: 600;\"\u003eInhibited under anaerobic conditions\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #4a5568;\"\u003e≤100 CFU\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #b8d0e0; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #0d2233; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e🧪 Typical Formulation (per litre)\u003c\/h4\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 8px; color: #2d3748; font-weight: 600;\"\u003eTryptone\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #4a5568; text-align: right;\"\u003e17.0 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 8px; color: #2d3748; font-weight: 600;\"\u003ePeptone\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #4a5568; text-align: right;\"\u003e5.0 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 8px; color: #2d3748; font-weight: 600;\"\u003eYeast Extract\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #4a5568; text-align: right;\"\u003e5.0 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 8px; color: #2d3748; font-weight: 600;\"\u003eGlucose (Dextrose)\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #4a5568; text-align: right;\"\u003e4.0 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 8px; color: #2d3748; font-weight: 600;\"\u003eSodium Thioglycollate\u003c\/td\u003e\n\u003ctd style=\"padding: 8px; color: #4a5568; text-align: right;\"\u003e1.0 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv style=\"font-size: 12px; color: #6c757d; margin-top: 10px; font-style: italic;\"\u003e* Formulation may vary slightly between lot numbers. Refer to the current COA for exact composition. Trypsin is NOT included.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #e3f2fd; padding: 20px; border-radius: 16px; border: 1px solid #90caf9; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #1565c0; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e📜 Standards \u0026amp; Regulatory Compliance\u003c\/h4\u003e\n\u003cul style=\"list-style: none; padding: 0; margin: 0;\"\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eFDA BAM Chapter 17\u003c\/strong\u003e — Clostridium botulinum enrichment in foods\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eAOAC 977.26\u003c\/strong\u003e — Clostridium botulinum and its toxins in foods\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eUSDA FSIS MLG 10\u003c\/strong\u003e — Detection of C. botulinum in meat and poultry products\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eAFNOR NF V08-062\u003c\/strong\u003e — Anaerobic enrichment of food samples\u003c\/li\u003e\n\u003cli style=\"padding: 8px 0; font-size: 14px; color: #1565c0;\"\u003e✓ \u003cstrong\u003eNATA Accreditation\u003c\/strong\u003e — Suitable for NATA-accredited food and reference laboratories\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #f0f4f8; padding: 20px; border-radius: 16px; border: 1px solid #b8d0e0; margin-bottom: 30px;\"\u003e\n\u003ch4 style=\"color: #1a3a52; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e📝 Alternative Names\u003c\/h4\u003e\n\u003cdiv style=\"font-size: 14px; color: #4a5568; text-align: center; line-height: 1.9;\"\u003e\n\u003cdiv\u003e\u003cstrong\u003eTPGY Broth Base (w\/o Trypsin)\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cstrong\u003eTryptone Peptone Glucose Yeast Extract Broth\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv\u003eTPGY Medium\u003c\/div\u003e\n\u003cdiv\u003eClostridium botulinum Enrichment Broth\u003c\/div\u003e\n\u003cdiv\u003eAnaerobic Spore-Former Enrichment Broth\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 20px; border-radius: 16px; border: 1px solid #b8d0e0;\"\u003e\n\u003ch4 style=\"color: #0d2233; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e📁 Documentation\u003c\/h4\u003e\n\u003cdiv style=\"display: flex; flex-direction: column; gap: 10px;\"\u003e\n\u003ca style=\"display: flex; align-items: center; gap: 10px; padding: 12px; background: #f0f4f8; border-radius: 10px; text-decoration: none; border: 1px solid #b8d0e0; color: #1a3a52; font-weight: 600; font-size: 14px;\" href=\"\/pages\/request-a-quote\"\u003e📄 Download Product Datasheet (TDS)\u003c\/a\u003e \u003ca style=\"display: flex; align-items: center; gap: 10px; padding: 12px; background: #f0f4f8; border-radius: 10px; text-decoration: none; border: 1px solid #b8d0e0; color: #1a3a52; font-weight: 600; font-size: 14px;\" href=\"\/pages\/request-a-quote\"\u003e⚠️ Download Safety Data Sheet (SDS)\u003c\/a\u003e \u003ca style=\"display: flex; align-items: center; gap: 10px; padding: 12px; background: #f0f4f8; border-radius: 10px; text-decoration: none; border: 1px solid #b8d0e0; color: #1a3a52; font-weight: 600; font-size: 14px;\" href=\"\/pages\/request-a-quote\"\u003e✓ Request Certificate of Analysis (COA)\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Cross-Reference --\u003e\n\u003cdiv style=\"background: #f0f4f8; padding: 30px; border-radius: 18px; border: 1px solid #b8d0e0; margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #0d2233; margin: 0 0 20px 0; text-align: center; font-size: 20px;\"\u003e🔄 Cross-Reference \/ Equivalent Products\u003c\/h3\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; font-size: 13px; border: 1px solid #b8d0e0; border-radius: 12px; overflow: hidden;\"\u003e\n\u003cthead style=\"background: #dce8f0;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"padding: 12px; text-align: left; color: #0d2233; font-weight: bold;\"\u003eSupplier\u003c\/th\u003e\n\u003cth style=\"padding: 12px; text-align: left; color: #0d2233; font-weight: bold;\"\u003eProduct Name\u003c\/th\u003e\n\u003cth style=\"padding: 12px; text-align: left; color: #0d2233; font-weight: bold;\"\u003eCatalogue Number\u003c\/th\u003e\n\u003cth style=\"padding: 12px; text-align: left; color: #0d2233; font-weight: bold;\"\u003eNotes\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #e0ecf5; border-bottom: 1px solid #b8d0e0;\"\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; color: #1a3a52;\"\u003eAuSaMicS \u003cspan style=\"background: #1a3a52; color: #fff; font-size: 10px; padding: 2px 6px; border-radius: 10px; margin-left: 4px;\"\u003eAU Stock\u003c\/span\u003e\n\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eTPGY Broth Base, w\/o Trypsin\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eAS-1366\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; font-size: 12px; color: #1a3a52;\"\u003eAustralian sourced, same-day dispatch\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eOxoid (Thermo Fisher)\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eTPGY Broth\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eCM0877\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; font-size: 12px; color: #6c757d;\"\u003eEquivalent formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eBD Difco\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eTryptone Peptone Glucose Yeast Broth\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003e299088\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; font-size: 12px; color: #6c757d;\"\u003eEquivalent formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom: 1px solid #e2e8f0;\"\u003e\n\u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eMerck \/ Millipore\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eTPGY Broth\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003e1.05359\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; font-size: 12px; color: #6c757d;\"\u003eEquivalent formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; font-weight: 600;\"\u003eNeogen\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003eTPGY Broth Base\u003c\/td\u003e\n\u003ctd style=\"padding: 12px;\"\u003e7485\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; font-size: 12px; color: #6c757d;\"\u003eEquivalent formulation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp style=\"font-size: 12px; color: #6c757d; margin-top: 12px; text-align: center; font-style: italic;\"\u003eCross-references are provided for convenience. Catalogue numbers are trademarks of their respective owners. AuSaMicS products are manufactured and quality-tested independently.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c!-- References \/ Literature --\u003e\n\u003cdiv style=\"background: #fff; padding: 25px; border-radius: 18px; border: 1px solid #b8d0e0; margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #0d2233; margin: 0 0 18px 0; font-size: 20px; text-align: center;\"\u003e📚 Literature \u0026amp; References\u003c\/h3\u003e\n\u003col style=\"margin: 0; padding-left: 22px; font-size: 13px; color: #4a5568; line-height: 2.0;\"\u003e\n\u003cli\u003eDezfulian M, Hatheway CL, Volpe JP, Dowell VR Jr. (1984). \u003cem\u003eCombination selective medium for isolation of Clostridium botulinum from human faeces.\u003c\/em\u003e J Clin Microbiol. 20(2):74–75.\u003c\/li\u003e\n\u003cli\u003eHauschild AHW, Dodds KL. (1993). \u003cem\u003eClostridium botulinum: Ecology and Control in Foods.\u003c\/em\u003e Marcel Dekker, New York.\u003c\/li\u003e\n\u003cli\u003eFDA Bacteriological Analytical Manual (BAM) Chapter 17: \u003cem\u003eClostridium botulinum.\u003c\/em\u003e U.S. Food and Drug Administration. Current edition.\u003c\/li\u003e\n\u003cli\u003eAOAC International (1995). \u003cem\u003eAOAC Official Method 977.26: Clostridium botulinum and its toxins in foods.\u003c\/em\u003e AOAC Int., Gaithersburg, MD.\u003c\/li\u003e\n\u003cli\u003eUSDA FSIS Microbiology Laboratory Guidebook (MLG) 10.00: \u003cem\u003eDetection of Clostridium botulinum in meat and poultry products.\u003c\/em\u003e Current edition.\u003c\/li\u003e\n\u003cli\u003eDowell VR Jr, McCroskey LM, Hatheway CL, Lombard GL, Hughes JM, Merson MH. (1977). \u003cem\u003eCoproexamination for botulinal toxin and Clostridium botulinum.\u003c\/em\u003e JAMA. 238(18):1829–1832.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003c!-- Related Products --\u003e\n\u003cdiv style=\"background: #f0f4f8; padding: 30px; border-radius: 18px; border: 1px solid #b8d0e0; margin-bottom: 30px;\"\u003e\n\u003ch3 style=\"color: #0d2233; margin: 0 0 20px 0; text-align: center; font-size: 20px;\"\u003e🧬 Complete Anaerobic \u0026amp; Botulism Testing System\u003c\/h3\u003e\n\u003cdiv style=\"margin-bottom: 25px;\"\u003e\n\u003ch4 style=\"color: #0d2233; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003e\n\u003cem\u003eClostridium\u003c\/em\u003e \u0026amp; Anaerobe Media Range\u003c\/h4\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit,minmax(175px,1fr)); gap: 15px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d0e0;\"\u003e\n\u003ch5 style=\"color: #1a3a52; margin: 0 0 8px 0; font-size: 14px;\"\u003eReinforced Clostridial Medium\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eNon-selective anaerobe recovery\u003c\/p\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"display: inline-block; background: #1a3a52; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d0e0;\"\u003e\n\u003ch5 style=\"color: #4a7fa5; margin: 0 0 8px 0; font-size: 14px;\"\u003eThioglycollate Broth (Fluid)\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eBroad-spectrum enrichment, sterility testing\u003c\/p\u003e\n\u003ca href=\"\/products\/fluid-thioglycollate-medium\" style=\"display: inline-block; background: #4a7fa5; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d0e0;\"\u003e\n\u003ch5 style=\"color: #6c757d; margin: 0 0 8px 0; font-size: 14px;\"\u003eCooked Meat Medium\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eAnaerobe preservation \u0026amp; enrichment\u003c\/p\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"display: inline-block; background: #6c757d; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d0e0;\"\u003e\n\u003ch5 style=\"color: #1a3a52; margin: 0 0 8px 0; font-size: 14px;\"\u003eDifferential Reinforced Clostridial Medium\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eSRC detection — water quality\u003c\/p\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"display: inline-block; background: #1a3a52; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 25px;\"\u003e\n\u003ch4 style=\"color: #0d2233; margin: 0 0 15px 0; font-size: 16px; text-align: center;\"\u003eFood Safety \u0026amp; Water Testing Range\u003c\/h4\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit,minmax(175px,1fr)); gap: 15px;\"\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d0e0;\"\u003e\n\u003ch5 style=\"color: #343a40; margin: 0 0 8px 0; font-size: 14px;\"\u003eSulfite Iron Agar Base\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003e\u003cem\u003eC. perfringens\u003c\/em\u003e — ISO 14189\u003c\/p\u003e\n\u003ca href=\"\/products\/sulfite-iron-agar-base\" style=\"display: inline-block; background: #343a40; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d0e0;\"\u003e\n\u003ch5 style=\"color: #0d6efd; margin: 0 0 8px 0; font-size: 14px;\"\u003eTSC Agar\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003e\u003cem\u003eC. perfringens\u003c\/em\u003e — ISO 7937 food\u003c\/p\u003e\n\u003ca href=\"\/pages\/request-a-quote\" style=\"display: inline-block; background: #0d6efd; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d0e0;\"\u003e\n\u003ch5 style=\"color: #1a6b3a; margin: 0 0 8px 0; font-size: 14px;\"\u003ePlate Count Agar\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eHPC — APHA 9215B\u003c\/p\u003e\n\u003ca href=\"\/products\/plate-count-agar-pca-standard-methods-agar-tryptone-glucose-yeast-agar\" style=\"display: inline-block; background: #1a6b3a; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"background: #fff; padding: 15px; border-radius: 12px; text-align: center; border: 1px solid #b8d0e0;\"\u003e\n\u003ch5 style=\"color: #17a2b8; margin: 0 0 8px 0; font-size: 14px;\"\u003eYeast Extract\u003c\/h5\u003e\n\u003cp style=\"margin: 0 0 10px 0; font-size: 12px; color: #6c757d;\"\u003eMedia supplement, food grade available\u003c\/p\u003e\n\u003ca href=\"\/products\/yeast-extract-powder\" style=\"display: inline-block; background: #17a2b8; color: #fff; text-decoration: none; padding: 6px 12px; border-radius: 6px; font-size: 11px;\"\u003eView Product\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"text-align: center; margin-top: 20px;\"\u003e\n\u003cdiv style=\"display: flex; justify-content: center; gap: 15px; flex-wrap: wrap;\"\u003e\n\u003ca href=\"\/collections\/culture-media\" style=\"color: #1a3a52; font-weight: 600; text-decoration: none; border: 2px solid #1a3a52; padding: 10px 20px; border-radius: 8px;\"\u003e🔬 All Culture Media\u003c\/a\u003e \u003ca href=\"\/collections\/culture-media\" style=\"color: #0d6efd; font-weight: 600; text-decoration: none; border: 2px solid #0d6efd; padding: 10px 20px; border-radius: 8px;\"\u003e🧬 Anaerobic Media Range\u003c\/a\u003e \u003ca href=\"\/pages\/request-a-quote\" style=\"color: #4a7fa5; font-weight: 600; text-decoration: none; border: 2px solid #4a7fa5; padding: 10px 20px; border-radius: 8px;\"\u003e📋 Request a Quote\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Footer \/ Contact --\u003e\n\u003cdiv style=\"text-align: center; padding: 30px 0; border-top: 1px solid #b8d0e0;\"\u003e\n\u003cdiv style=\"margin-bottom: 20px;\"\u003e\n\u003ch4 style=\"color: #0d2233; margin: 0 0 10px 0; font-size: 16px;\"\u003eNeed \u003cem\u003eClostridium botulinum\u003c\/em\u003e Testing Method Support?\u003c\/h4\u003e\n\u003cp style=\"margin: 0; color: #6c757d; font-size: 14px;\"\u003eOur food and anaerobic microbiology specialists can assist with FDA BAM Chapter 17 workflows, trypsin activation protocols, mouse bioassay and ELISA toxin detection, anaerobic incubation system selection, heat-shock spore enrichment, and NATA documentation for botulism reference testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: flex; justify-content: center; gap: 20px; flex-wrap: wrap; margin-bottom: 25px;\"\u003e\n\u003ca href=\"tel:+61412520598\" style=\"display: flex; align-items: center; gap: 8px; color: #1a3a52; text-decoration: none; font-weight: 600; padding: 12px 20px; border: 1px solid #1a3a52; border-radius: 10px;\"\u003e📞 +61 412 520 598\u003c\/a\u003e \u003ca href=\"mailto:support@ausamics.com.au\" style=\"display: flex; align-items: center; gap: 8px; color: #1a3a52; text-decoration: none; font-weight: 600; padding: 12px 20px; border: 1px solid #1a3a52; border-radius: 10px;\"\u003e📧 Technical Support\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cp style=\"margin: 0; font-size: 12px; color: #94a3b8; line-height: 1.6; font-style: italic; border: 1px solid #b8d0e0; padding: 12px 20px; border-radius: 8px; display: inline-block; max-width: 720px;\"\u003e\u003cstrong\u003eFor laboratory, research, and industrial use only.\u003c\/strong\u003e Not for food, feed, household, cosmetic, therapeutic, or personal use. Results should be interpreted by qualified laboratory personnel in accordance with applicable standard methods. Strictly anaerobic incubation conditions are required for valid results. Trypsin is not included and must be sourced separately; follow all applicable biosafety procedures when handling potentially toxigenic cultures.\u003cbr\u003e\u003cbr\u003eAuSaMicS Pty Ltd | ABN: 56 676 640 467 | 31 Longview CT, Thomastown VIC 3074, Australia\u003cbr\u003ewww.ausamics.com.au | support@ausamics.com.au | +61 412 520 598\u003cbr\u003eSame-day dispatch • Australian stock • Full documentation included\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- end aus-tpgy-product --\u003e\n\u003cstyle\u003e\n@media (max-width: 860px) {\n  .aus-tpgy-hero-grid, .aus-tpgy-main-grid { grid-template-columns: 1fr !important; }\n  .aus-tpgy-guarantee { min-width: 100% !important; margin-top: 20px; }\n  .aus-tpgy-product { padding: 15px !important; }\n  .aus-tpgy-product h1 { font-size: 24px !important; }\n  .aus-tpgy-product h2 { font-size: 18px !important; }\n}\n@media (max-width: 640px) {\n  .aus-tpgy-hero-section { padding: 25px !important; }\n  .aus-tpgy-product table th, .aus-tpgy-product table td { padding: 8px 6px !important; }\n}\n\u003c\/style\u003e","brand":"Ausamics Life Science","offers":[{"title":"100 g","offer_id":48379498103039,"sku":"AS-1366-100G","price":37.0,"currency_code":"AUD","in_stock":true},{"title":"500 g","offer_id":48379498135807,"sku":"AS-1366-500G","price":111.0,"currency_code":"AUD","in_stock":true}],"url":"https:\/\/ausamics.com.au\/products\/tpgy-broth-base-w-o-trypsin","provider":"AuSaMicS","version":"1.0","type":"link"}