{"product_id":"tryptone-water-tw","title":"Tryptone Water (TW)","description":"\u003cp data-start=\"295\" data-end=\"391\"\u003e\u003cstrong data-start=\"295\" data-end=\"318\"\u003eTryptone Water (TW)\u003c\/strong\u003e\u003cbr data-start=\"318\" data-end=\"321\"\u003eIndole Test Broth for Biochemical Identification of Enteric Bacteria\u003c\/p\u003e\n\u003cp data-start=\"393\" data-end=\"422\"\u003e\u003cstrong data-start=\"393\" data-end=\"412\"\u003eCatalog Number:\u003c\/strong\u003e AS-1367\u003c\/p\u003e\n\u003chr data-start=\"424\" data-end=\"427\"\u003e\n\u003cp data-start=\"429\" data-end=\"815\"\u003e\u003cstrong data-start=\"429\" data-end=\"441\"\u003eOverview\u003c\/strong\u003e\u003cbr data-start=\"441\" data-end=\"444\"\u003eTryptone Water (TW) is a simple, nutrient-rich liquid culture medium used primarily for the detection of indole production and for the general cultivation of non-fastidious microorganisms. The medium contains tryptone as a source of amino acids and peptides, supporting microbial growth and enabling organisms that produce tryptophanase to convert tryptophan into indole.\u003c\/p\u003e\n\u003cp data-start=\"817\" data-end=\"990\"\u003eTryptone Water is widely used in clinical diagnostics, microbiological research, and food and water testing, particularly for biochemical identification of enteric bacteria.\u003c\/p\u003e\n\u003chr data-start=\"992\" data-end=\"995\"\u003e\n\u003cp data-start=\"997\" data-end=\"1252\"\u003e\u003cstrong data-start=\"997\" data-end=\"1013\"\u003eApplications\u003c\/strong\u003e\u003cbr data-start=\"1013\" data-end=\"1016\"\u003e• Detection of indole production from bacterial cultures\u003cbr data-start=\"1072\" data-end=\"1075\"\u003e• General cultivation of non-fastidious microorganisms\u003cbr data-start=\"1129\" data-end=\"1132\"\u003e• Biochemical testing and identification of enteric bacteria\u003cbr data-start=\"1192\" data-end=\"1195\"\u003e• Research and educational applications in microbiology\u003c\/p\u003e\n\u003chr data-start=\"1254\" data-end=\"1257\"\u003e\n\u003cp data-start=\"1259\" data-end=\"1604\"\u003e\u003cstrong data-start=\"1259\" data-end=\"1286\"\u003eKey Features \u0026amp; Benefits\u003c\/strong\u003e\u003cbr data-start=\"1286\" data-end=\"1289\"\u003e• Simple, nutrient-rich formulation supporting growth of a wide range of bacteria\u003cbr data-start=\"1370\" data-end=\"1373\"\u003e• Compatible with indole detection using Kovac’s reagent and similar reagents\u003cbr data-start=\"1450\" data-end=\"1453\"\u003e• Non-selective medium suitable for non-fastidious organisms\u003cbr data-start=\"1513\" data-end=\"1516\"\u003e• Versatile use in biochemical assays, routine microbiology, and teaching laboratories\u003c\/p\u003e\n\u003chr data-start=\"1606\" data-end=\"1609\"\u003e\n\u003cp data-start=\"1611\" data-end=\"2021\"\u003e\u003cstrong data-start=\"1611\" data-end=\"1638\"\u003ePrinciple of the Medium\u003c\/strong\u003e\u003cbr data-start=\"1638\" data-end=\"1641\"\u003eTryptone Water provides peptides and amino acids derived from tryptone, allowing bacteria to grow and metabolize tryptophan. Organisms capable of producing tryptophanase convert tryptophan into indole, which can be detected by the addition of Kovac’s reagent, producing a characteristic pink to red colour. Growth in the medium indicates organism viability and metabolic activity.\u003c\/p\u003e\n\u003chr data-start=\"2023\" data-end=\"2026\"\u003e\n\u003cp data-start=\"2028\" data-end=\"2142\"\u003e\u003cstrong data-start=\"2028\" data-end=\"2063\"\u003eTypical Composition (per litre)\u003c\/strong\u003e\u003cbr data-start=\"2063\" data-end=\"2066\"\u003eTryptone – 10.0 g\u003cbr data-start=\"2083\" data-end=\"2086\"\u003eSodium Chloride – 5.0 g\u003cbr data-start=\"2109\" data-end=\"2112\"\u003ePurified Water – q.s. to 1 L\u003c\/p\u003e\n\u003cp data-start=\"2144\" data-end=\"2174\"\u003eFinal pH: 7.3 ± 0.2 at 25 °C\u003c\/p\u003e\n\u003cp data-start=\"2176\" data-end=\"2260\"\u003e(Composition may be adjusted to meet specific institutional or published standards.)\u003c\/p\u003e\n\u003chr data-start=\"2262\" data-end=\"2265\"\u003e\n\u003cp data-start=\"2267\" data-end=\"2548\"\u003e\u003cstrong data-start=\"2267\" data-end=\"2282\"\u003ePreparation\u003c\/strong\u003e\u003cbr data-start=\"2282\" data-end=\"2285\"\u003eSuspend 15 g of dehydrated medium in 1 L of purified water.\u003cbr data-start=\"2344\" data-end=\"2347\"\u003eHeat with gentle agitation until completely dissolved.\u003cbr data-start=\"2401\" data-end=\"2404\"\u003eDispense into tubes, flasks, or bottles as required.\u003cbr data-start=\"2456\" data-end=\"2459\"\u003eSterilize by autoclaving at 121 °C for 15 minutes.\u003cbr data-start=\"2509\" data-end=\"2512\"\u003eCool to room temperature before use.\u003c\/p\u003e\n\u003chr data-start=\"2550\" data-end=\"2553\"\u003e\n\u003cp data-start=\"2555\" data-end=\"2670\"\u003e\u003cstrong data-start=\"2555\" data-end=\"2586\"\u003eIncubation \u0026amp; Interpretation\u003c\/strong\u003e\u003cbr data-start=\"2586\" data-end=\"2589\"\u003eIncubate at 35–37 °C for 18–24 hours (up to 48 hours for slow-growing organisms).\u003c\/p\u003e\n\u003cp data-start=\"2672\" data-end=\"2813\"\u003ePositive indole reaction: Pink or red colour after addition of Kovac’s reagent\u003cbr data-start=\"2750\" data-end=\"2753\"\u003eNegative reaction: No colour change after reagent addition\u003c\/p\u003e\n\u003chr data-start=\"2815\" data-end=\"2818\"\u003e\n\u003cp data-start=\"2820\" data-end=\"3048\"\u003e\u003cstrong data-start=\"2820\" data-end=\"2843\"\u003eStorage \u0026amp; Stability\u003c\/strong\u003e\u003cbr data-start=\"2843\" data-end=\"2846\"\u003eDehydrated medium: Store at 15–30 °C in a dry, tightly sealed container\u003cbr data-start=\"2917\" data-end=\"2920\"\u003ePrepared medium: Store at 2–8 °C, protected from light\u003cbr data-start=\"2974\" data-end=\"2977\"\u003eShelf life: Use within the recommended period for optimal performance\u003c\/p\u003e\n\u003chr data-start=\"3050\" data-end=\"3053\"\u003e\n\u003cp data-start=\"3055\" data-end=\"3186\"\u003e\u003cstrong data-start=\"3055\" data-end=\"3071\"\u003eIntended Use\u003c\/strong\u003e\u003cbr data-start=\"3071\" data-end=\"3074\"\u003eFor laboratory research and diagnostic use only.\u003cbr data-start=\"3122\" data-end=\"3125\"\u003eNot for human, veterinary, food, feed, or pharmaceutical use.\u003c\/p\u003e\n\u003chr data-start=\"3188\" data-end=\"3191\"\u003e\n\u003cp data-start=\"3193\" data-end=\"3446\"\u003e\u003cstrong data-start=\"3193\" data-end=\"3217\"\u003eQuality \u0026amp; Compliance\u003c\/strong\u003e\u003cbr data-start=\"3217\" data-end=\"3220\"\u003eManufactured under controlled conditions to ensure batch-to-batch consistency.\u003cbr data-start=\"3298\" data-end=\"3301\"\u003eSuitable for clinical, food, water, and research microbiology.\u003cbr data-start=\"3363\" data-end=\"3366\"\u003ePerformance comparable to leading international microbiological media suppliers.\u003c\/p\u003e\n\u003chr data-start=\"3448\" data-end=\"3451\"\u003e\n\u003cp data-start=\"3453\" data-end=\"3594\"\u003e\u003cstrong data-start=\"3453\" data-end=\"3484\"\u003eCustoms \u0026amp; Trade Information\u003c\/strong\u003e\u003cbr data-start=\"3484\" data-end=\"3487\"\u003eHS \/ AHECC Code: 3821.00.00\u003cbr data-start=\"3514\" data-end=\"3517\"\u003ePrepared culture media for the development or maintenance of microorganisms\u003c\/p\u003e","brand":"Ausamics Life Science","offers":[{"title":"100 g","offer_id":48147236094207,"sku":"AS-1367-100G","price":29.0,"currency_code":"AUD","in_stock":true}],"url":"https:\/\/ausamics.com.au\/products\/tryptone-water-tw","provider":"AuSaMicS","version":"1.0","type":"link"}