Agarose Powder | Molecular Biology Grade Gel Electrophoresis Agarose

Agarose Powder — High-Purity Molecular Biology Grade

For DNA & RNA Gel Electrophoresis

Catalog Number: AS-1015
CAS Number: 9012-36-6

Overview

Agarose Powder is a high-purity polysaccharide derived from refined seaweed, specifically designed for molecular biology applications. It is the standard solid matrix used for DNA and RNA gel electrophoresis, providing excellent resolution, reproducibility, and ease of use.

Ausamics Agarose Powder offers high gel strength and low electroendosmosis (EEO), ensuring sharp band separation and consistent migration of nucleic acids. It is suitable for routine and advanced workflows including PCR analysis, restriction enzyme digestion, cloning, and nucleic acid quality control.

Key Specifications

• Gel Strength (1.0%): ≥ 1200 g/cm²

• Moisture Content: ≤ 10.0 % (w/w)

• EEO (–Mr): ≤ 0.15

• Gelling Point (1.5%): 35–37 °C

• Melting Point (1.5%): 87–89 °C

Key Features & Benefits

• High-purity molecular biology grade

• Low EEO for clear, well-resolved DNA and RNA bands

• Consistent gel strength and pore size

• Compatible with TAE and TBE buffers

• Suitable for routine, teaching, and research laboratories

Typical Applications

Molecular Biology & Genetics

• DNA and RNA gel electrophoresis

• Analysis of PCR products

• Restriction enzyme digestion analysis

• Plasmid and genomic DNA quality assessment

Microbiology & Mycology Research

• Supporting molecular analysis in environmental, food, and water studies

• Complementary use with microbiological media and reagents

Education & Training

• University teaching laboratories

• Practical demonstrations of electrophoresis principles

• Student and training workflows

Principle of Use

Agarose consists of repeating galactose-based polysaccharide units that form a thermoreversible gel when heated in aqueous buffer and cooled. The resulting porous gel matrix allows nucleic acids to migrate under an electric field according to fragment size.

Pore size can be adjusted by concentration:

• Higher concentrations (e.g. 2.0%) → smaller pores for small DNA fragments

• Lower concentrations (e.g. 0.8%) → larger pores for high-molecular-weight DNA

Instructions for Use

1. Weigh the required amount of agarose powder

2. Suspend in appropriate electrophoresis buffer (TAE or TBE)

3. Heat until fully dissolved

4. Cool slightly and pour into a gel casting tray

5. Allow to solidify before loading samples

Storage & Stability

• Store in a cool, dry place

• Protect from moisture and direct sunlight

• Keep container tightly closed when not in use

Available Pack Sizes

• 5 g – 100 g: Suitable for small laboratories and teaching use

• 500 g: Ideal for high-throughput research and institutional laboratories

Bulk quantities available upon request.

Intended Use

For laboratory and research use only.
Not for food, pharmaceutical, diagnostic, or veterinary applications.

Quality & Documentation

• Supplied with Certificate of Analysis (COA)

• Safety Data Sheet (SDS) available

• Manufactured and quality-controlled for consistent batch performance

Customs & Trade Information

HS / AHECC Code: 1302.39
Agar and vegetable mucilages